Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005871

Physical Location & Seq

Genomic Coordinates Reverse (-)

14387194 .. 14390339

Gene Structure 3146 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G34070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1158 bp

ATGGGTTTCAAACTCAACTCTCTTTTCCTTCTTCTTTCGCTTCTCATACTCACAATCTTGTCCGGAGCCGACCAAGCCTTGGCTTACGTCAAGAAACCACATGTGAGCCAACGCAACAAAACGGCGTTAGCAGCCGTCGCCGGAAGAGGAGGAGGAAAAGAGATGTTGAAAGGGAGGAAGCAGACGAGTGGTTGTAACTTGTTTCAAGGGAGATGGGTTTTCGATGCTTCTTACCCTTTCTACGATTCATCCACGTGCCCTTTCATCGACGGTGAGTTCGACTGTCTCAAATTCGGCCGACCAGACAAACAGTTCCTTAAGTACTCTTGGCAGCCTGATTCATGCACCGTCCCAAGGTTTGATGGGGAAGCGTTTTTGAAGAAATGGAGAGGGAAACGAGTGATGTTCGTGGGTGACTCACTGAGTCTAAACATGTGGGAATCGTTGGCATGTATGATACATTCGTCGGTTCCAAACACTAAGACCACTTTTCTCAAGCGAACCCCACTCTCCTCTCTCACTTTCCAGGAATATGACGTCACACTATTCCTATACCGAACACCATACCTAGTGGACATCTCCAAAGAAAGTGTCGGGCGTGTGCTTAACCTTGGAGCCATTGAAGATGGAGCTGATGCTTGGAAAAATATGGACCTCCTCGTCTTCAATTCTTGGCACTGGTGGACTCACACAGGAGTACAGTCTCAAGGGTGGGATTTTATAAGAGATGGGTCTTCACTGATGAGAGACATGGACCGCCTTGATGCTTTCAACAAAGGACTCACCACTTGGGGTCAATGGGTTGATCAAAATGTTAATGTTTCGCAAACCCGAGTCTTCTTCCAAGGCATTTCTCCCACTCACTACATGGGAAGGGAATGGAACGAGCCGAGGAAAACTTGCAACGGGCAGATGCAACCGTTGACCGGATCAACATACCCAGGTGGTTCACTTCCTGCAGCAAGCATTGTGTCTCGAGTATTAAGCACGATGAGAACGCCCGTTTACTTACTCGACATCACAACTCTATCTCAACTGAGAAAAGATGCTCATCCATCTACATATGGAGGCGATGGCGGAACGGATTGCAGTCACTGGTGCCTTCCTGGCTTGCCGGATACTTGGAACCAGCTTCTCTATGCAGCTCTTTCGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005911 GO:0008150 GO:0008374 GO:0009506 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

385

Amino Acids

43.35

Weight (kDa)

8.8

Isoelectric Point (pI)

37.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	64 - 115	1.8e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	117 - 381	2.5e-94	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29050 AT2G34070 AT2G34070
fragaria_vesca	FvH4_3g07990 FvH4_3g07990 FvH4_3g17800 FvH4_3g17800
malus_domestica	MD05G1303400.v1.1 MD10G1282500.v1.1
prunus_persica	Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1
pyrus_communis	pycom05g28040 pycom10g23560
rosa_chinensis	RchiOBHm_Chr5g0010331
rosa_laevigata	RLG00000031738
rosa_multiflora	Rmu_co8488479.1_g000001 Rmu_sc0007816.1_g000002
rosa_roxburghii	Rroxscaffold_1G00065920
rosa_rugosa	Rorug04G0450100
rosa_samantha	Rh5AG081400 Rh5BG076300 Rh5BG206000 Rh5CG089600 Rh5DG077000
rosa_wichuraiana	Rw5G007540 Rw5G018870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	722
AasI	GACNNNNNNGTC	1 cut(s)	659
AatII	GACGTC	1 cut(s)	540
AccB1I	GGYRCC	1 cut(s)	1098
AccB7I	CCANNNNNTGG	1 cut(s)	79
AccIII	TCCGGA	1 cut(s)	62
AciI	CCGC	2 cut(s)	757, 1077
AclWI	GGATC	1 cut(s)	937
AcoI	YGGCCR	1 cut(s)	295
AcsI	RAATTY	1 cut(s)	290
AcvI	CACGTG	1 cut(s)	255
AcyI	GRCGYC	1 cut(s)	537
AfaI	GTAC	2 cut(s)	323, 699
AfiI	CCNNNNNNNGG	3 cut(s)	79, 354, 926
AflII	CTTAAG	1 cut(s)	317
AflIII	ACRYGT	2 cut(s)	100, 432
AgsI	TTSAA	7 cut(s)	10, 169, 206, 379, 623, 667, 772
AjnI	CCWGG	3 cut(s)	525, 940, 1105
AluBI	AGCT	3 cut(s)	632, 1132, 1145
AluI	AGCT	3 cut(s)	632, 1132, 1145
Alw26I	GTCTC	4 cut(s)	290, 708, 741, 978
AlwI	GGATC	1 cut(s)	937
AlwNI	CAGNNNCTG	1 cut(s)	1095
Ama87I	CYCGRG	2 cut(s)	831, 975
Aor13HI	TCCGGA	1 cut(s)	62
AoxI	GGCC	1 cut(s)	295
ApeKI	GCWGC	4 cut(s)	131, 331, 959, 1142
ApoI	RAATTY	1 cut(s)	290
ArsI	GACNNNNNNTTYG	4 cut(s)	260, 292, 576, 608
AspS9I	GGNCC	2 cut(s)	652, 754
AsuHPI	GGTGA	3 cut(s)	284, 425, 775
AvaI	CYCGRG	2 cut(s)	831, 975
AvaII	GGWCC	2 cut(s)	652, 754
BaeGI	GKGCMC	1 cut(s)	260
BaeI	ACNNNNGTAYC	2 cut(s)	449, 482
BanI	GGYRCC	1 cut(s)	1098
BbrPI	CACGTG	1 cut(s)	255
BbsI	GAAGAC	3 cut(s)	655, 726, 829
BbvI	GCAGC	4 cut(s)	143, 343, 971, 1154
BccI	CCATC	6 cut(s)	207, 356, 620, 722, 1063, 1067
BceAI	ACGGC	2 cut(s)	119, 138
BcgI	CGANNNNNNTGC	1 cut(s)	1131
BciT130I	CCWGG	3 cut(s)	527, 942, 1107
BciVI	GTATCC	1 cut(s)	1111
BclI	TGATCA	1 cut(s)	805
BcoDI	GTCTC	4 cut(s)	290, 708, 741, 978
BfaI	CTAG	1 cut(s)	569
BfmI	CTRYAG	1 cut(s)	957
BfrI	CTTAAG	1 cut(s)	317
BfuI	GTATCC	1 cut(s)	1111
BglI	GCCNNNNNGGC	1 cut(s)	1107
BisI	GCNGC	4 cut(s)	132, 332, 960, 1143
BlsI	GCNGC	4 cut(s)	133, 333, 961, 1144
BmcAI	AGTACT	1 cut(s)	323
Bme1390I	CCNGG	3 cut(s)	527, 942, 1107
Bme18I	GGWCC	2 cut(s)	652, 754
BmeT110I	CYCGRG	2 cut(s)	831, 975
BmgT120I	GGNCC	2 cut(s)	652, 754
BmiI	GGNNCC	5 cut(s)	67, 471, 616, 1100, 1127
BmrFI	CCNGG	3 cut(s)	527, 942, 1107
BmsI	GCATC	5 cut(s)	214, 625, 754, 903, 1036
BpiI	GAAGAC	3 cut(s)	655, 726, 829
BpuEI	CTTGAG	2 cut(s)	479, 690
BsaAI	YACGTR	1 cut(s)	255
BsaBI	GATNNNNATC	1 cut(s)	1050
BsaHI	GRCGYC	1 cut(s)	537
BsaJI	CCNNGG	6 cut(s)	78, 353, 610, 844, 890, 940
BsaWI	WCCGGW	2 cut(s)	62, 926
Bsc4I	CCNNNNNNNGG	3 cut(s)	79, 354, 926
Bse1I	ACTGG	2 cut(s)	683, 1100
Bse8I	GATNNNNATC	1 cut(s)	1050
BseAI	TCCGGA	1 cut(s)	62
BseBI	CCWGG	3 cut(s)	527, 942, 1107
BseDI	CCNNGG	6 cut(s)	78, 353, 610, 844, 890, 940
BseGI	GGATG	2 cut(s)	248, 1051
BseJI	GATNNNNATC	1 cut(s)	1050
BseLI	CCNNNNNNNGG	3 cut(s)	79, 354, 926
BseMII	CTCAG	2 cut(s)	413, 1028
BseNI	ACTGG	2 cut(s)	683, 1100
BseRI	GAGGAG	4 cut(s)	162, 165, 502, 647
BseSI	GKGCMC	1 cut(s)	260
BseX3I	CGGCCG	1 cut(s)	295
BseXI	GCAGC	4 cut(s)	143, 343, 971, 1154
Bsh1285I	CGRYCG	1 cut(s)	298
BshFI	GGCC	1 cut(s)	297
BshNI	GGYRCC	1 cut(s)	1098
BsiEI	CGRYCG	1 cut(s)	298
BsiHKCI	CYCGRG	2 cut(s)	831, 975
BsiSI	CCGG	4 cut(s)	63, 141, 927, 1115
BslFI	GGGAC	1 cut(s)	335
BslI	CCNNNNNNNGG	3 cut(s)	79, 354, 926
BsmAI	GTCTC	4 cut(s)	290, 708, 741, 978
BsmFI	GGGAC	1 cut(s)	335
BsnI	GGCC	1 cut(s)	297
BsoBI	CYCGRG	2 cut(s)	831, 975
Bsp1286I	GDGCHC	1 cut(s)	260
Bsp13I	TCCGGA	1 cut(s)	62
Bsp143I	GATC	2 cut(s)	805, 929
BspACI	CCGC	2 cut(s)	757, 1077
BspANI	GGCC	1 cut(s)	297
BspCNI	CTCAG	2 cut(s)	414, 1029
BspEI	TCCGGA	1 cut(s)	62
BspLI	GGNNCC	5 cut(s)	67, 471, 616, 1100, 1127
BspMAI	CTGCAG	1 cut(s)	961
BspPI	GGATC	1 cut(s)	937
BspT107I	GGYRCC	1 cut(s)	1098
BspTI	CTTAAG	1 cut(s)	317
BsrI	ACTGG	2 cut(s)	683, 1100
BssECI	CCNNGG	6 cut(s)	78, 353, 610, 844, 890, 940
BssMI	GATC	2 cut(s)	805, 929
BssNI	GRCGYC	1 cut(s)	537
BssT1I	CCWWGG	4 cut(s)	78, 353, 610, 844
Bst2UI	CCWGG	3 cut(s)	527, 942, 1107
Bst4CI	ACNGT	6 cut(s)	272, 284, 312, 349, 702, 921
Bst6I	CTCTTC	1 cut(s)	139
BstACI	GRCGYC	1 cut(s)	537
BstAFI	CTTAAG	1 cut(s)	317
BstBAI	YACGTR	1 cut(s)	255
BstC8I	GCNNGC	2 cut(s)	964, 1112
BstDEI	CTNAG	3 cut(s)	422, 480, 1037
BstF5I	GGATG	2 cut(s)	248, 1051
BstKTI	GATC	2 cut(s)	808, 932
BstMAI	GTCTC	4 cut(s)	290, 708, 741, 978
BstMBI	GATC	2 cut(s)	805, 929
BstMCI	CGRYCG	1 cut(s)	298
BstMWI	GCNNNNNNNGC	4 cut(s)	74, 131, 137, 1107
BstNI	CCWGG	3 cut(s)	527, 942, 1107
BstNSI	RCATGY	3 cut(s)	104, 436, 453
BstSCI	CCNGG	3 cut(s)	525, 940, 1105
BstSFI	CTRYAG	1 cut(s)	957
BstSLI	GKGCMC	1 cut(s)	260
BstV1I	GCAGC	4 cut(s)	143, 343, 971, 1154
BstV2I	GAAGAC	3 cut(s)	655, 726, 829
BstZI	CGGCCG	1 cut(s)	295
BsuI	GTATCC	1 cut(s)	1111
BsuRI	GGCC	1 cut(s)	297
BtgZI	GCGATG	1 cut(s)	1086
BtsCI	GGATG	2 cut(s)	248, 1051
BtsIMutI	CAGTG	4 cut(s)	419, 676, 737, 1093
Cac8I	GCNNGC	2 cut(s)	964, 1112
CaiI	CAGNNNCTG	1 cut(s)	1095
Cfr13I	GGNCC	2 cut(s)	652, 754
Csp6I	GTAC	2 cut(s)	322, 698
CviAII	CATG	6 cut(s)	101, 342, 433, 450, 751, 868
CviQI	GTAC	2 cut(s)	322, 698
DdeI	CTNAG	3 cut(s)	422, 480, 1037
DpnI	GATC	2 cut(s)	807, 931
DpnII	GATC	2 cut(s)	805, 929
DrdI	GACNNNNNNGTC	1 cut(s)	659
DseDI	GACNNNNNNGTC	1 cut(s)	659
EaeI	YGGCCR	1 cut(s)	295
EagI	CGGCCG	1 cut(s)	295
Eam1104I	CTCTTC	1 cut(s)	139
EarI	CTCTTC	1 cut(s)	139
EciI	GGCGGA	1 cut(s)	1092
EclXI	CGGCCG	1 cut(s)	295
Eco130I	CCWWGG	4 cut(s)	78, 353, 610, 844
Eco47I	GGWCC	2 cut(s)	652, 754
Eco52I	CGGCCG	1 cut(s)	295
Eco72I	CACGTG	1 cut(s)	255
Eco88I	CYCGRG	2 cut(s)	831, 975
EcoRII	CCWGG	3 cut(s)	525, 940, 1105
EcoT14I	CCWWGG	4 cut(s)	78, 353, 610, 844
ErhI	CCWWGG	4 cut(s)	78, 353, 610, 844
FaeI	CATG	6 cut(s)	104, 345, 436, 453, 754, 871
FaqI	GGGAC	1 cut(s)	335
FatI	CATG	6 cut(s)	100, 341, 432, 449, 750, 867
FauNDI	CATATG	1 cut(s)	1063
FbaI	TGATCA	1 cut(s)	805
Fnu4HI	GCNGC	4 cut(s)	132, 332, 960, 1143
FokI	GGATG	2 cut(s)	235, 1038
Fsp4HI	GCNGC	4 cut(s)	132, 332, 960, 1143
FspBI	CTAG	1 cut(s)	569
GluI	GCNGC	4 cut(s)	132, 332, 960, 1143
HaeIII	GGCC	1 cut(s)	297
HapII	CCGG	4 cut(s)	63, 141, 927, 1115
Hin1I	GRCGYC	1 cut(s)	537
Hin1II	CATG	6 cut(s)	104, 345, 436, 453, 754, 871
HincII	GTYRAC	1 cut(s)	924
HindII	GTYRAC	1 cut(s)	924
HinfI	GANTC	8 cut(s)	245, 338, 416, 424, 440, 685, 780, 834
HpaII	CCGG	4 cut(s)	63, 141, 927, 1115
HphI	GGTGA	3 cut(s)	284, 425, 775
Hpy166II	GTNNAC	5 cut(s)	574, 684, 924, 950, 1006
Hpy188III	TCNNGA	3 cut(s)	63, 91, 975
Hpy8I	GTNNAC	5 cut(s)	574, 684, 924, 950, 1006
Hpy99I	CGWCG	3 cut(s)	140, 272, 469
HpyAV	CCTTC	3 cut(s)	38, 867, 1112
HpyCH4III	ACNGT	6 cut(s)	272, 284, 312, 349, 702, 921
HpyCH4IV	ACGT	3 cut(s)	87, 254, 537
HpyCH4V	TGCA	6 cut(s)	345, 903, 916, 959, 1089, 1142
HpyF10VI	GCNNNNNNNGC	4 cut(s)	74, 131, 137, 1107
HpyF3I	CTNAG	3 cut(s)	422, 480, 1037
HpySE526I	ACGT	3 cut(s)	87, 254, 537
Hsp92I	GRCGYC	1 cut(s)	537
Hsp92II	CATG	6 cut(s)	104, 345, 436, 453, 754, 871
Kpn2I	TCCGGA	1 cut(s)	62
Ksp22I	TGATCA	1 cut(s)	805
Kzo9I	GATC	2 cut(s)	805, 929
LmnI	GCTCC	3 cut(s)	65, 614, 629
Lsp1109I	GCAGC	4 cut(s)	143, 343, 971, 1154
LweI	GCATC	5 cut(s)	214, 625, 754, 903, 1036
MaeI	CTAG	1 cut(s)	569
MaeII	ACGT	3 cut(s)	87, 254, 537
MaeIII	GTNAC	4 cut(s)	194, 413, 538, 1091
MalI	GATC	2 cut(s)	807, 931
MboI	GATC	2 cut(s)	805, 929
MboII	GAAGA	8 cut(s)	23, 156, 391, 635, 655, 726, 829, 832
MhlI	GDGCHC	1 cut(s)	260
MluCI	AATT	2 cut(s)	290, 667
MlyI	GAGTC	5 cut(s)	410, 433, 679, 774, 843
MroI	TCCGGA	1 cut(s)	62
MseI	TTAA	4 cut(s)	318, 606, 816, 983
MslI	CAYNNNNRTG	1 cut(s)	253
MspCI	CTTAAG	1 cut(s)	317
MspI	CCGG	4 cut(s)	63, 141, 927, 1115
MspR9I	CCNGG	3 cut(s)	527, 942, 1107
MvaI	CCWGG	3 cut(s)	527, 942, 1107
MwoI	GCNNNNNNNGC	4 cut(s)	74, 131, 137, 1107
NdeI	CATATG	1 cut(s)	1063
NdeII	GATC	2 cut(s)	805, 929
NlaIII	CATG	6 cut(s)	104, 345, 436, 453, 754, 871
NlaIV	GGNNCC	5 cut(s)	67, 471, 616, 1100, 1127
NmeAIII	GCCGAG	1 cut(s)	915
NmuCI	GTSAC	3 cut(s)	413, 538, 1091
NspI	RCATGY	3 cut(s)	104, 436, 453
PaeR7I	CTCGAG	1 cut(s)	975
PciI	ACATGT	2 cut(s)	100, 432
PcsI	WCGNNNNNNNCGW	1 cut(s)	276
PfeI	GAWTC	3 cut(s)	245, 338, 440
PflMI	CCANNNNNTGG	1 cut(s)	79
PfoI	TCCNGGA	1 cut(s)	525
PkrI	GCNGC	4 cut(s)	133, 333, 961, 1144
PleI	GAGTC	5 cut(s)	410, 432, 679, 774, 842
PmaCI	CACGTG	1 cut(s)	255
PmlI	CACGTG	1 cut(s)	255
PpsI	GAGTC	5 cut(s)	410, 432, 679, 774, 842
Ppu21I	YACGTR	1 cut(s)	255
PscI	ACATGT	2 cut(s)	100, 432
PsiI	TTATAA	1 cut(s)	722
Psp6I	CCWGG	3 cut(s)	525, 940, 1105
PspCI	CACGTG	1 cut(s)	255
PspGI	CCWGG	3 cut(s)	525, 940, 1105
PspN4I	GGNNCC	5 cut(s)	67, 471, 616, 1100, 1127
PspPI	GGNCC	2 cut(s)	652, 754
PstI	CTGCAG	1 cut(s)	961
PstNI	CAGNNNCTG	1 cut(s)	1095
RsaI	GTAC	2 cut(s)	323, 699
RsaNI	GTAC	2 cut(s)	322, 698
RseI	CAYNNNNRTG	1 cut(s)	253
SaqAI	TTAA	4 cut(s)	318, 606, 816, 983
SatI	GCNGC	4 cut(s)	132, 332, 960, 1143
Sau3AI	GATC	2 cut(s)	805, 929
Sau96I	GGNCC	2 cut(s)	652, 754
ScaI	AGTACT	1 cut(s)	323
SchI	GAGTC	5 cut(s)	410, 433, 679, 774, 843
ScrFI	CCNGG	3 cut(s)	527, 942, 1107
SduI	GDGCHC	1 cut(s)	260
SfaNI	GCATC	5 cut(s)	214, 625, 754, 903, 1036
SfcI	CTRYAG	1 cut(s)	957
Sfr274I	CTCGAG	1 cut(s)	975
SinI	GGWCC	2 cut(s)	652, 754
SlaI	CTCGAG	1 cut(s)	975
SmiMI	CAYNNNNRTG	1 cut(s)	253
SmlI	CTYRAG	4 cut(s)	317, 494, 705, 975
SmoI	CTYRAG	4 cut(s)	317, 494, 705, 975
Sse9I	AATT	2 cut(s)	290, 667
SsiI	CCGC	2 cut(s)	757, 1077
SspMI	CTAG	1 cut(s)	569
StyD4I	CCNGG	3 cut(s)	525, 940, 1105
StyI	CCWWGG	4 cut(s)	78, 353, 610, 844
TaaI	ACNGT	6 cut(s)	272, 284, 312, 349, 702, 921
TaiI	ACGT	3 cut(s)	90, 257, 540
TaqI	TCGA	6 cut(s)	222, 267, 279, 976, 1014, 1151
TasI	AATT	2 cut(s)	290, 667
TatI	WGTACW	2 cut(s)	321, 697
TfiI	GAWTC	3 cut(s)	245, 338, 440
Tru1I	TTAA	4 cut(s)	318, 606, 816, 983
Tru9I	TTAA	4 cut(s)	318, 606, 816, 983
TscAI	CASTG	4 cut(s)	426, 683, 744, 1100
TseFI	GTSAC	3 cut(s)	413, 538, 1091
TseI	GCWGC	4 cut(s)	131, 331, 959, 1142
Tsp45I	GTSAC	3 cut(s)	413, 538, 1091
TspDTI	ATGAA	3 cut(s)	237, 253, 330
TspGWI	ACGGA	1 cut(s)	1097
TspRI	CASTG	4 cut(s)	426, 683, 744, 1100
Van91I	CCANNNNNTGG	1 cut(s)	79
Vha464I	CTTAAG	1 cut(s)	317
VpaK11BI	GGWCC	2 cut(s)	652, 754
XapI	RAATTY	1 cut(s)	290
XceI	RCATGY	3 cut(s)	104, 436, 453
XcmI	CCANNNNNNNNNTGG	1 cut(s)	865
XhoI	CTCGAG	1 cut(s)	975
XspI	CTAG	1 cut(s)	569
ZraI	GACGTC	1 cut(s)	538
ZrmI	AGTACT	1 cut(s)	323

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.