Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005871

Physical Location & Seq

Genomic Coordinates Forward (+)

37316382 .. 37320678

Gene Structure 4297 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1282500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGGTTTCAAGCTCCAAGCTCCGCTGTGCCTCTTAGCTCTTCAAGTTCTGGTCTTCTTCTCAGAAGAAGCAGAGTCAGCAGGGCACAGCCATGGCAGCAGCTTGAGAGTCCACACGAAGCAAGTGAGCCGCTGCAATTTGTTCGAAGGCAATTGGGTTTTTGATGCTTCTTACCCTCTTTATGATTCTGCCACCTGTCCCTTCATAGATCCTGAGTTTGATTGCATCAAGTATGGCAGACCTGATACGCAGTTCCTCAAGTATGCCTGGAAGCCCGACTCCTGTGACTTGCCCAGGTTTGATGGGGTGGATTTTCTGAGGAGGTGGAGAGGAAAGAAGATAATGTTTGTGGGCGACTCACTGAGTCTGAACATGTGGGAATCGTTGTCTTGTATGATACATGCGTCGGTGCCAGATGCCAAGACTACCTTCAGAAATGGCCATTCTGTGAACTTTGAGGACTATGGAGTGACTTTGTTTATGTTCCGGACACCGTACCTTGTAGATATAGTCAGAGAAGAAGTTGGCCGTGTTCTAAACCTAAACTCCATCAACGCCGGAGATGCATGGAAAGGGATGGACGTCCTGATCTTCAACTCTTGGCATTGGTGGACCCACACCGGCGATTCTCAACCATTTGATTATTTTCGGGATGGGACAGAACTGTACAAAGATATGGATCGTTTGACAGCATTTTATAAGGGGCTTTCCACATGGGCTAAATGGGTTGACTCAAATGTTGATACTTCAAGAACCAAAGTCTTCTTCCAGGGCATTTCTCCAACACATTACCAGGGACAAGAATGGAATTCGCCGAAGAAGACTTGCTTAGGAGAACTTGGACCGCTATCGGGATCAACCTACCCAGCAGGGGCACCTCCATCTGCTGCTGTTGTCTATAAGGTGCTGAGCGCGATTAAGAGCCCGGTTTACTTGCTCGACATTACAATGCTCTCGCAGTTGAGGAAGGATGCTCATCCCTCGACTTACAGCGGCGATCATTCGGGCAACGACTGCAGCCATTGGTGCTTGCCAGGGTTGCCCGATACCTGGAACCAACTCCTATACGCAGCTCTCATCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

362

Amino Acids

40.92

Weight (kDa)

5.99

Isoelectric Point (pI)

36.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	44 - 96	3e-23	PMR5 N terminal Domain
PC-Esterase	PF13839	97 - 357	4.1e-91	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29050 AT2G34070 AT2G34070
fragaria_vesca	FvH4_3g07990 FvH4_3g07990 FvH4_3g17800 FvH4_3g17800
malus_domestica	MD05G1303400.v1.1 MD10G1282500.v1.1
prunus_persica	Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1
pyrus_communis	pycom05g28040 pycom10g23560
rosa_chinensis	RchiOBHm_Chr5g0010331
rosa_laevigata	RLG00000031738
rosa_multiflora	Rmu_co8488479.1_g000001 Rmu_sc0007816.1_g000002
rosa_roxburghii	Rroxscaffold_1G00065920
rosa_rugosa	Rorug04G0450100
rosa_samantha	Rh5AG081400 Rh5BG076300 Rh5BG206000 Rh5CG089600 Rh5DG077000
rosa_wichuraiana	Rw5G007540 Rw5G018870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	699
AatII	GACGTC	1 cut(s)	585
AccB1I	GGYRCC	2 cut(s)	409, 874
AccII	CGCG	1 cut(s)	914
AccIII	TCCGGA	1 cut(s)	486
AciI	CCGC	4 cut(s)	23, 130, 845, 993
AclWI	GGATC	3 cut(s)	203, 687, 862
AcoI	YGGCCR	2 cut(s)	439, 526
AcsI	RAATTY	1 cut(s)	808
AcuI	CTGAAG	1 cut(s)	415
AcyI	GRCGYC	1 cut(s)	582
AfaI	GTAC	2 cut(s)	497, 668
AfiI	CCNNNNNNNGG	3 cut(s)	851, 871, 1050
AflIII	ACRYGT	1 cut(s)	372
AgsI	TTSAA	4 cut(s)	10, 44, 595, 750
AjnI	CCWGG	6 cut(s)	266, 293, 768, 792, 1033, 1049
AjuI	GAANNNNNNNTTGG	4 cut(s)	413, 445, 749, 781
AluBI	AGCT	5 cut(s)	13, 20, 38, 102, 1073
AluI	AGCT	5 cut(s)	13, 20, 38, 102, 1073
AlwI	GGATC	3 cut(s)	203, 687, 862
Aor13HI	TCCGGA	1 cut(s)	486
AoxI	GGCC	2 cut(s)	439, 526
ApeKI	GCWGC	6 cut(s)	96, 99, 132, 887, 1017, 1070
ApoI	RAATTY	1 cut(s)	808
AspLEI	GCGC	1 cut(s)	914
AspS9I	GGNCC	2 cut(s)	612, 842
AsuC2I	CCSGG	1 cut(s)	926
AsuII	TTCGAA	1 cut(s)	144
AvaII	GGWCC	2 cut(s)	612, 842
BaeGI	GKGCMC	2 cut(s)	87, 877
BaeI	ACNNNNGTAYC	2 cut(s)	389, 422
BalI	TGGCCA	1 cut(s)	441
BanI	GGYRCC	2 cut(s)	409, 874
BanII	GRGCYC	1 cut(s)	926
BbsI	GAAGAC	3 cut(s)	46, 754, 827
BbvI	GCAGC	6 cut(s)	108, 111, 119, 874, 1029, 1082
BccI	CCATC	5 cut(s)	296, 557, 571, 647, 889
BceAI	ACGGC	1 cut(s)	513
BcgI	CGANNNNNNTGC	2 cut(s)	124, 158
BciT130I	CCWGG	6 cut(s)	268, 295, 770, 794, 1035, 1051
BcnI	CCSGG	1 cut(s)	926
BfmI	CTRYAG	1 cut(s)	1015
BisI	GCNGC	8 cut(s)	97, 100, 130, 133, 888, 994, 1018, 1071
BlpI	GCTNAGC	1 cut(s)	908
BlsI	GCNGC	8 cut(s)	98, 101, 131, 134, 889, 995, 1019, 1072
Bme1390I	CCNGG	7 cut(s)	268, 295, 770, 794, 926, 1035, 1051
Bme18I	GGWCC	2 cut(s)	612, 842
BmgT120I	GGNCC	2 cut(s)	612, 842
BmiI	GGNNCC	4 cut(s)	411, 614, 876, 1055
BmrFI	CCNGG	7 cut(s)	268, 295, 770, 794, 926, 1035, 1051
BmsI	GCATC	5 cut(s)	154, 234, 406, 553, 961
BpiI	GAAGAC	3 cut(s)	46, 754, 827
Bpu10I	CCTNAGC	1 cut(s)	829
Bpu1102I	GCTNAGC	1 cut(s)	908
Bpu14I	TTCGAA	1 cut(s)	144
BpuEI	CTTGAG	2 cut(s)	124, 242
BpuMI	CCSGG	1 cut(s)	926
BsaBI	GATNNNNATC	2 cut(s)	678, 975
BsaHI	GRCGYC	1 cut(s)	582
BsaJI	CCNNGG	5 cut(s)	91, 293, 769, 793, 1034
BsaWI	WCCGGW	1 cut(s)	486
BsaXI	ACNNNNNCTCC	2 cut(s)	530, 560
Bsc4I	CCNNNNNNNGG	3 cut(s)	851, 871, 1050
Bse118I	RCCGGY	1 cut(s)	620
Bse8I	GATNNNNATC	2 cut(s)	678, 975
BseAI	TCCGGA	1 cut(s)	486
BseBI	CCWGG	6 cut(s)	268, 295, 770, 794, 1035, 1051
BseDI	CCNNGG	5 cut(s)	91, 293, 769, 793, 1034
BseGI	GGATG	4 cut(s)	582, 658, 976, 976
BseJI	GATNNNNATC	2 cut(s)	678, 975
BseLI	CCNNNNNNNGG	3 cut(s)	851, 871, 1050
BseMII	CTCAG	5 cut(s)	75, 204, 308, 353, 899
BseRI	GAGGAG	1 cut(s)	334
BseSI	GKGCMC	2 cut(s)	87, 877
BseXI	GCAGC	6 cut(s)	108, 111, 119, 874, 1029, 1082
BseYI	CCCAGC	1 cut(s)	865
Bsh1236I	CGCG	1 cut(s)	914
BshFI	GGCC	2 cut(s)	441, 528
BshNI	GGYRCC	2 cut(s)	409, 874
BsiSI	CCGG	4 cut(s)	487, 558, 621, 926
BslFI	GGGAC	3 cut(s)	183, 670, 810
BslI	CCNNNNNNNGG	3 cut(s)	851, 871, 1050
BsmFI	GGGAC	3 cut(s)	183, 670, 810
BsnI	GGCC	2 cut(s)	441, 528
Bsp119I	TTCGAA	1 cut(s)	144
Bsp1286I	GDGCHC	3 cut(s)	87, 877, 926
Bsp13I	TCCGGA	1 cut(s)	486
Bsp1407I	TGTACA	1 cut(s)	666
Bsp143I	GATC	5 cut(s)	208, 588, 679, 854, 997
Bsp1720I	GCTNAGC	1 cut(s)	908
Bsp19I	CCATGG	1 cut(s)	91
BspACI	CCGC	4 cut(s)	23, 130, 845, 993
BspANI	GGCC	2 cut(s)	441, 528
BspCNI	CTCAG	5 cut(s)	74, 205, 309, 354, 900
BspEI	TCCGGA	1 cut(s)	486
BspFNI	CGCG	1 cut(s)	914
BspLI	GGNNCC	4 cut(s)	411, 614, 876, 1055
BspMAI	CTGCAG	1 cut(s)	1019
BspPI	GGATC	3 cut(s)	203, 687, 862
BspQI	GCTCTTC	1 cut(s)	45
BspT104I	TTCGAA	1 cut(s)	144
BspT107I	GGYRCC	2 cut(s)	409, 874
BsrFI	RCCGGY	1 cut(s)	620
BsrGI	TGTACA	1 cut(s)	666
BssAI	RCCGGY	1 cut(s)	620
BssECI	CCNNGG	5 cut(s)	91, 293, 769, 793, 1034
BssMI	GATC	5 cut(s)	208, 588, 679, 854, 997
BssNI	GRCGYC	1 cut(s)	582
BssT1I	CCWWGG	1 cut(s)	91
Bst2UI	CCWGG	6 cut(s)	268, 295, 770, 794, 1035, 1051
Bst4CI	ACNGT	2 cut(s)	495, 666
Bst6I	CTCTTC	1 cut(s)	45
BstACI	GRCGYC	1 cut(s)	582
BstAPI	GCANNNNNTGC	1 cut(s)	1014
BstAUI	TGTACA	1 cut(s)	666
BstBI	TTCGAA	1 cut(s)	144
BstC8I	GCNNGC	1 cut(s)	1031
BstDEI	CTNAG	7 cut(s)	34, 61, 213, 317, 362, 829, 908
BstDSI	CCRYGG	1 cut(s)	91
BstF5I	GGATG	4 cut(s)	582, 658, 976, 976
BstFNI	CGCG	1 cut(s)	914
BstHHI	GCGC	1 cut(s)	914
BstKTI	GATC	5 cut(s)	211, 591, 682, 857, 1000
BstMBI	GATC	5 cut(s)	208, 588, 679, 854, 997
BstMWI	GCNNNNNNNGC	6 cut(s)	77, 96, 563, 1014, 1026, 1039
BstNI	CCWGG	6 cut(s)	268, 295, 770, 794, 1035, 1051
BstNSI	RCATGY	2 cut(s)	376, 404
BstSCI	CCNGG	7 cut(s)	266, 293, 768, 792, 924, 1033, 1049
BstSFI	CTRYAG	1 cut(s)	1015
BstSLI	GKGCMC	2 cut(s)	87, 877
BstUI	CGCG	1 cut(s)	914
BstV1I	GCAGC	6 cut(s)	108, 111, 119, 874, 1029, 1082
BstV2I	GAAGAC	3 cut(s)	46, 754, 827
BstX2I	RGATCY	1 cut(s)	208
BstYI	RGATCY	1 cut(s)	208
BsuRI	GGCC	2 cut(s)	441, 528
BtgI	CCRYGG	1 cut(s)	91
BtsCI	GGATG	4 cut(s)	582, 658, 976, 976
BtsIMutI	CAGTG	1 cut(s)	359
Cac8I	GCNNGC	1 cut(s)	1031
CfoI	GCGC	1 cut(s)	914
Cfr10I	RCCGGY	1 cut(s)	620
Cfr13I	GGNCC	2 cut(s)	612, 842
CseI	GACGC	1 cut(s)	393
Csp6I	GTAC	2 cut(s)	496, 667
CviAII	CATG	6 cut(s)	92, 373, 401, 567, 714, 1081
CviQI	GTAC	2 cut(s)	496, 667
DdeI	CTNAG	7 cut(s)	34, 61, 213, 317, 362, 829, 908
DpnI	GATC	5 cut(s)	210, 590, 681, 856, 999
DpnII	GATC	5 cut(s)	208, 588, 679, 854, 997
EaeI	YGGCCR	2 cut(s)	439, 526
Eam1104I	CTCTTC	1 cut(s)	45
EarI	CTCTTC	1 cut(s)	45
Eco130I	CCWWGG	1 cut(s)	91
Eco24I	GRGCYC	1 cut(s)	926
Eco47I	GGWCC	2 cut(s)	612, 842
Eco57I	CTGAAG	1 cut(s)	415
EcoRI	GAATTC	1 cut(s)	808
EcoRII	CCWGG	6 cut(s)	266, 293, 768, 792, 1033, 1049
EcoT14I	CCWWGG	1 cut(s)	91
EcoT22I	ATGCAT	1 cut(s)	568
EcoT38I	GRGCYC	1 cut(s)	926
ErhI	CCWWGG	1 cut(s)	91
FaeI	CATG	6 cut(s)	95, 376, 404, 570, 717, 1084
FalI	AAGNNNNNCTT	4 cut(s)	413, 445, 812, 844
FaqI	GGGAC	3 cut(s)	183, 670, 810
FatI	CATG	6 cut(s)	91, 372, 400, 566, 713, 1080
Fnu4HI	GCNGC	8 cut(s)	97, 100, 130, 133, 888, 994, 1018, 1071
FokI	GGATG	4 cut(s)	589, 665, 963, 983
FriOI	GRGCYC	1 cut(s)	926
Fsp4HI	GCNGC	8 cut(s)	97, 100, 130, 133, 888, 994, 1018, 1071
GlaI	GCGC	1 cut(s)	913
GluI	GCNGC	8 cut(s)	97, 100, 130, 133, 888, 994, 1018, 1071
GsaI	CCCAGC	1 cut(s)	869
HaeIII	GGCC	2 cut(s)	441, 528
HapII	CCGG	4 cut(s)	487, 558, 621, 926
HgaI	GACGC	1 cut(s)	393
HhaI	GCGC	1 cut(s)	914
Hin1I	GRCGYC	1 cut(s)	582
Hin1II	CATG	6 cut(s)	95, 376, 404, 570, 717, 1084
Hin6I	GCGC	1 cut(s)	912
HinP1I	GCGC	1 cut(s)	912
HincII	GTYRAC	1 cut(s)	730
HindII	GTYRAC	1 cut(s)	730
HinfI	GANTC	9 cut(s)	74, 108, 185, 278, 356, 364, 380, 626, 731
HpaII	CCGG	4 cut(s)	487, 558, 621, 926
Hpy166II	GTNNAC	5 cut(s)	112, 451, 612, 730, 931
Hpy188I	TCNGA	5 cut(s)	64, 318, 369, 434, 515
Hpy188III	TCNNGA	6 cut(s)	212, 487, 586, 650, 750, 852
Hpy8I	GTNNAC	5 cut(s)	112, 451, 612, 730, 931
Hpy99I	CGWCG	1 cut(s)	409
HpyAV	CCTTC	4 cut(s)	140, 211, 439, 961
HpyCH4III	ACNGT	2 cut(s)	495, 666
HpyCH4IV	ACGT	1 cut(s)	582
HpyCH4V	TGCA	4 cut(s)	135, 225, 566, 1017
HpyF10VI	GCNNNNNNNGC	6 cut(s)	77, 96, 563, 1014, 1026, 1039
HpyF3I	CTNAG	7 cut(s)	34, 61, 213, 317, 362, 829, 908
HpySE526I	ACGT	1 cut(s)	582
Hsp92I	GRCGYC	1 cut(s)	582
Hsp92II	CATG	6 cut(s)	95, 376, 404, 570, 717, 1084
HspAI	GCGC	1 cut(s)	912
Kpn2I	TCCGGA	1 cut(s)	486
Kzo9I	GATC	5 cut(s)	208, 588, 679, 854, 997
LguI	GCTCTTC	1 cut(s)	45
LmnI	GCTCC	2 cut(s)	18, 25
Lsp1109I	GCAGC	6 cut(s)	108, 111, 119, 874, 1029, 1082
LweI	GCATC	5 cut(s)	154, 234, 406, 553, 961
MaeII	ACGT	1 cut(s)	582
MaeIII	GTNAC	2 cut(s)	284, 469
MalI	GATC	5 cut(s)	210, 590, 681, 856, 999
MboI	GATC	5 cut(s)	208, 588, 679, 854, 997
MfeI	CAATTG	1 cut(s)	151
MflI	RGATCY	1 cut(s)	208
MhlI	GDGCHC	3 cut(s)	87, 877, 926
MlsI	TGGCCA	1 cut(s)	441
MluCI	AATT	3 cut(s)	136, 151, 808
MluNI	TGGCCA	1 cut(s)	441
MlyI	GAGTC	6 cut(s)	83, 117, 272, 350, 373, 725
MmeI	TCCRAC	1 cut(s)	806
Mox20I	TGGCCA	1 cut(s)	441
Mph1103I	ATGCAT	1 cut(s)	568
MroI	TCCGGA	1 cut(s)	486
MscI	TGGCCA	1 cut(s)	441
MseI	TTAA	1 cut(s)	918
MslI	CAYNNNNRTG	2 cut(s)	90, 947
Msp20I	TGGCCA	1 cut(s)	441
MspA1I	CMGCKG	3 cut(s)	25, 132, 993
MspI	CCGG	4 cut(s)	487, 558, 621, 926
MspR9I	CCNGG	7 cut(s)	268, 295, 770, 794, 926, 1035, 1051
MunI	CAATTG	1 cut(s)	151
MvaI	CCWGG	6 cut(s)	268, 295, 770, 794, 1035, 1051
MvnI	CGCG	1 cut(s)	914
MwoI	GCNNNNNNNGC	6 cut(s)	77, 96, 563, 1014, 1026, 1039
NciI	CCSGG	1 cut(s)	926
NcoI	CCATGG	1 cut(s)	91
NdeII	GATC	5 cut(s)	208, 588, 679, 854, 997
NlaIII	CATG	6 cut(s)	95, 376, 404, 570, 717, 1084
NlaIV	GGNNCC	4 cut(s)	411, 614, 876, 1055
NmuCI	GTSAC	2 cut(s)	284, 469
NsiI	ATGCAT	1 cut(s)	568
NspI	RCATGY	2 cut(s)	376, 404
NspV	TTCGAA	1 cut(s)	144
PciI	ACATGT	1 cut(s)	372
PciSI	GCTCTTC	1 cut(s)	45
PfeI	GAWTC	3 cut(s)	185, 380, 626
PkrI	GCNGC	8 cut(s)	98, 101, 131, 134, 889, 995, 1019, 1072
PleI	GAGTC	6 cut(s)	82, 116, 272, 350, 372, 725
PpsI	GAGTC	6 cut(s)	82, 116, 272, 350, 372, 725
PscI	ACATGT	1 cut(s)	372
PsiI	TTATAA	1 cut(s)	699
Psp6I	CCWGG	6 cut(s)	266, 293, 768, 792, 1033, 1049
PspFI	CCCAGC	1 cut(s)	865
PspGI	CCWGG	6 cut(s)	266, 293, 768, 792, 1033, 1049
PspN4I	GGNNCC	4 cut(s)	411, 614, 876, 1055
PspPI	GGNCC	2 cut(s)	612, 842
PstI	CTGCAG	1 cut(s)	1019
PsuI	RGATCY	1 cut(s)	208
RsaI	GTAC	2 cut(s)	497, 668
RsaNI	GTAC	2 cut(s)	496, 667
RseI	CAYNNNNRTG	2 cut(s)	90, 947
SapI	GCTCTTC	1 cut(s)	45
SaqAI	TTAA	1 cut(s)	918
SatI	GCNGC	8 cut(s)	97, 100, 130, 133, 888, 994, 1018, 1071
Sau3AI	GATC	5 cut(s)	208, 588, 679, 854, 997
Sau96I	GGNCC	2 cut(s)	612, 842
SchI	GAGTC	6 cut(s)	83, 117, 272, 350, 373, 725
ScrFI	CCNGG	7 cut(s)	268, 295, 770, 794, 926, 1035, 1051
SduI	GDGCHC	3 cut(s)	87, 877, 926
SfaNI	GCATC	5 cut(s)	154, 234, 406, 553, 961
SfcI	CTRYAG	1 cut(s)	1015
SfuI	TTCGAA	1 cut(s)	144
SgrAI	CRCCGGYG	1 cut(s)	620
SinI	GGWCC	2 cut(s)	612, 842
SmiMI	CAYNNNNRTG	2 cut(s)	90, 947
SmlI	CTYRAG	2 cut(s)	103, 257
SmoI	CTYRAG	2 cut(s)	103, 257
Sse9I	AATT	3 cut(s)	136, 151, 808
SsiI	CCGC	4 cut(s)	23, 130, 845, 993
StyD4I	CCNGG	7 cut(s)	266, 293, 768, 792, 924, 1033, 1049
StyI	CCWWGG	1 cut(s)	91
TaaI	ACNGT	2 cut(s)	495, 666
TaiI	ACGT	1 cut(s)	585
TaqI	TCGA	3 cut(s)	144, 939, 983
TasI	AATT	3 cut(s)	136, 151, 808
TatI	WGTACW	1 cut(s)	666
TauI	GCSGC	2 cut(s)	132, 996
TfiI	GAWTC	3 cut(s)	185, 380, 626
Tru1I	TTAA	1 cut(s)	918
Tru9I	TTAA	1 cut(s)	918
TscAI	CASTG	1 cut(s)	366
TseFI	GTSAC	2 cut(s)	284, 469
TseI	GCWGC	6 cut(s)	96, 99, 132, 887, 1017, 1070
Tsp45I	GTSAC	2 cut(s)	284, 469
TspDTI	ATGAA	1 cut(s)	193
TspRI	CASTG	1 cut(s)	366
VpaK11BI	GGWCC	2 cut(s)	612, 842
XapI	RAATTY	1 cut(s)	808
XceI	RCATGY	2 cut(s)	376, 404
ZraI	GACGTC	1 cut(s)	583
Zsp2I	ATGCAT	1 cut(s)	568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.