Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005871

Physical Location & Seq

Genomic Coordinates Forward (+)

43593091 .. 43597070

Gene Structure 3980 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1303400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGGTTTCAAGCTCCAAGCTCTGCTGTGCCTTGTAGCTCTTCAAGTTCTGGTCTTCTTCTCAGAGGAAGCAGAAGCAGCAGAACACAGCCATGGCATCAGCTTGAGAGTCCACAGGAAGCAAGTGAGCGGCTGCAATTTGTTCCAAGGCAATTGGGTTTTTGATGCTTCTTACCCGCTCTATGATTCCGCCAGCTGTCCCTTCATAGATCCTGAGTTTGATTGCATCAAGTATGGCAGACCTGACAAGCAGTTCCTCAAGTATGCCTGGAAGCCTGACTCCTGTAACTTGCCCAGGTTTGATGGGGTGGATTTTCTGAGGAGGTGGAGGGGAAAAAAGATAATGTTCGTGGGCGACTCACTGAGTCTAAACATGTGGGAATCATTGTCTTGTATGATACATGCGTCGGTGCCAAATGCCAAGACCACCTTCAAAAAAGCCTATTCTGTGAGCTTTGAGGACTATGGAGTGACTTTGTTTATGTTCCGGACACCATACCTTGTAGATATAGTCAGAGAAAAGGTTGGCCGTGTGCTAAACCTAAACTCCATCAACGCCGGAAATGCATGGAAAGGGATGGACGTCCTGATCTTCAACTCTTGGCATTGGTGGACCCACACCGGCAAGTCTCAGCCATTTGATTATTTTCGTGATGGGACAAAACTGTACAAAGACATGGATCGTTTGACAGCATATTATAAGGGGCTTTCCACATGGGCTAGATGGGTTGACTCAAATGTTGATACTTCAAGAACCAAAGTCTTCTTCCAGGGCATTTCTCCAACACATTATCAGGGAAAAGAATGGAATTCGCCAAAGAAGACTTGCTCAGGAGAACTTGGACCGTTGTCGGGATCAACCTACCCAGCAGGGGCACCTCCATCTGCTGCTGTTGTGTATAAGGTGCTAAGCACGATTAAGAACCCGGTTTACTTGCTCGACATTACAACGCTCTCGCAGTTGAGAAAGGATGCTCATCCCTCAACTTATAGTGGCGATCATTCGGGCAATGACTGCAGCCACTGGTGCCTGCCCGGGTTGCCTGATACCTGGAACCAACTCCTATATGCAGCTCTCATCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

362

Amino Acids

41.03

Weight (kDa)

8.71

Isoelectric Point (pI)

31.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	44 - 96	4.4e-23	PMR5 N terminal Domain
PC-Esterase	PF13839	97 - 357	5.4e-93	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29050 AT2G34070 AT2G34070
fragaria_vesca	FvH4_3g07990 FvH4_3g07990 FvH4_3g17800 FvH4_3g17800
malus_domestica	MD05G1303400.v1.1 MD10G1282500.v1.1
prunus_persica	Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1 Prupe.4G059400_v2.0.a1
pyrus_communis	pycom05g28040 pycom10g23560
rosa_chinensis	RchiOBHm_Chr5g0010331
rosa_laevigata	RLG00000031738
rosa_multiflora	Rmu_co8488479.1_g000001 Rmu_sc0007816.1_g000002
rosa_roxburghii	Rroxscaffold_1G00065920
rosa_rugosa	Rorug04G0450100
rosa_samantha	Rh5AG081400 Rh5BG076300 Rh5BG206000 Rh5CG089600 Rh5DG077000
rosa_wichuraiana	Rw5G007540 Rw5G018870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	699
AatII	GACGTC	1 cut(s)	585
AccB1I	GGYRCC	3 cut(s)	409, 874, 1026
AccBSI	CCGCTC	2 cut(s)	129, 178
AccIII	TCCGGA	1 cut(s)	486
AciI	CCGC	3 cut(s)	129, 176, 189
AclWI	GGATC	3 cut(s)	203, 687, 862
AcoI	YGGCCR	1 cut(s)	526
AcsI	RAATTY	1 cut(s)	808
AcyI	GRCGYC	1 cut(s)	582
AfaI	GTAC	1 cut(s)	668
AfiI	CCNNNNNNNGG	2 cut(s)	851, 871
AflIII	ACRYGT	1 cut(s)	372
AgsI	TTSAA	5 cut(s)	10, 44, 433, 595, 750
AjnI	CCWGG	4 cut(s)	266, 293, 768, 1049
AjuI	GAANNNNNNNTTGG	4 cut(s)	413, 445, 749, 781
AluBI	AGCT	7 cut(s)	13, 20, 38, 102, 195, 453, 1073
AluI	AGCT	7 cut(s)	13, 20, 38, 102, 195, 453, 1073
Alw26I	GTCTC	1 cut(s)	633
AlwI	GGATC	3 cut(s)	203, 687, 862
AlwNI	CAGNNNCTG	1 cut(s)	1023
Ama87I	CYCGRG	1 cut(s)	1034
Aor13HI	TCCGGA	1 cut(s)	486
AoxI	GGCC	1 cut(s)	526
ApeKI	GCWGC	5 cut(s)	77, 132, 887, 1017, 1070
ApoI	RAATTY	1 cut(s)	808
AspS9I	GGNCC	2 cut(s)	612, 842
AsuC2I	CCSGG	3 cut(s)	926, 1035, 1036
AvaI	CYCGRG	1 cut(s)	1034
AvaII	GGWCC	2 cut(s)	612, 842
BaeGI	GKGCMC	1 cut(s)	877
BaeI	ACNNNNGTAYC	2 cut(s)	389, 422
BanI	GGYRCC	3 cut(s)	409, 874, 1026
BbsI	GAAGAC	3 cut(s)	46, 754, 827
BbvI	GCAGC	5 cut(s)	89, 119, 874, 1029, 1082
BccI	CCATC	6 cut(s)	296, 557, 571, 647, 717, 889
BceAI	ACGGC	1 cut(s)	513
BciT130I	CCWGG	4 cut(s)	268, 295, 770, 1051
BcnI	CCSGG	3 cut(s)	926, 1035, 1036
BcoDI	GTCTC	1 cut(s)	633
BfaI	CTAG	1 cut(s)	720
BfmI	CTRYAG	1 cut(s)	1015
BisI	GCNGC	6 cut(s)	78, 130, 133, 888, 1018, 1071
BlpI	GCTNAGC	1 cut(s)	908
BlsI	GCNGC	6 cut(s)	79, 131, 134, 889, 1019, 1072
Bme1390I	CCNGG	7 cut(s)	268, 295, 770, 926, 1035, 1036, 1051
Bme18I	GGWCC	2 cut(s)	612, 842
BmeT110I	CYCGRG	1 cut(s)	1034
BmgT120I	GGNCC	2 cut(s)	612, 842
BmiI	GGNNCC	5 cut(s)	411, 614, 876, 1028, 1055
BmrFI	CCNGG	7 cut(s)	268, 295, 770, 926, 1035, 1036, 1051
BmsI	GCATC	4 cut(s)	105, 154, 234, 961
BoxI	GACNNNNGTC	1 cut(s)	847
BpiI	GAAGAC	3 cut(s)	46, 754, 827
Bpu10I	CCTNAGC	1 cut(s)	829
Bpu1102I	GCTNAGC	1 cut(s)	908
BpuEI	CTTGAG	2 cut(s)	124, 242
BpuMI	CCSGG	3 cut(s)	926, 1035, 1036
BsaBI	GATNNNNATC	1 cut(s)	975
BsaHI	GRCGYC	1 cut(s)	582
BsaJI	CCNNGG	5 cut(s)	91, 145, 293, 769, 1034
BsaWI	WCCGGW	1 cut(s)	486
BsaXI	ACNNNNNCTCC	2 cut(s)	530, 560
Bsc4I	CCNNNNNNNGG	2 cut(s)	851, 871
Bse118I	RCCGGY	1 cut(s)	620
Bse1I	ACTGG	1 cut(s)	1028
Bse3DI	GCAATG	1 cut(s)	1015
Bse8I	GATNNNNATC	1 cut(s)	975
BseAI	TCCGGA	1 cut(s)	486
BseBI	CCWGG	4 cut(s)	268, 295, 770, 1051
BseDI	CCNNGG	5 cut(s)	91, 145, 293, 769, 1034
BseGI	GGATG	3 cut(s)	582, 976, 976
BseJI	GATNNNNATC	1 cut(s)	975
BseLI	CCNNNNNNNGG	2 cut(s)	851, 871
BseMI	GCAATG	1 cut(s)	1015
BseMII	CTCAG	6 cut(s)	75, 204, 308, 353, 644, 843
BseNI	ACTGG	1 cut(s)	1028
BseRI	GAGGAG	1 cut(s)	334
BseSI	GKGCMC	1 cut(s)	877
BseXI	GCAGC	5 cut(s)	89, 119, 874, 1029, 1082
BseYI	CCCAGC	1 cut(s)	865
BshFI	GGCC	1 cut(s)	528
BshNI	GGYRCC	3 cut(s)	409, 874, 1026
BsiHKCI	CYCGRG	1 cut(s)	1034
BsiSI	CCGG	5 cut(s)	487, 558, 621, 926, 1035
BslFI	GGGAC	2 cut(s)	183, 670
BslI	CCNNNNNNNGG	2 cut(s)	851, 871
BsmAI	GTCTC	1 cut(s)	633
BsmFI	GGGAC	2 cut(s)	183, 670
BsnI	GGCC	1 cut(s)	528
BsoBI	CYCGRG	1 cut(s)	1034
Bsp1286I	GDGCHC	1 cut(s)	877
Bsp13I	TCCGGA	1 cut(s)	486
Bsp1407I	TGTACA	1 cut(s)	666
Bsp143I	GATC	5 cut(s)	208, 588, 679, 854, 997
Bsp1720I	GCTNAGC	1 cut(s)	908
Bsp19I	CCATGG	1 cut(s)	91
BspACI	CCGC	3 cut(s)	129, 176, 189
BspANI	GGCC	1 cut(s)	528
BspCNI	CTCAG	6 cut(s)	74, 205, 309, 354, 643, 842
BspEI	TCCGGA	1 cut(s)	486
BspLI	GGNNCC	5 cut(s)	411, 614, 876, 1028, 1055
BspMAI	CTGCAG	1 cut(s)	1019
BspPI	GGATC	3 cut(s)	203, 687, 862
BspQI	GCTCTTC	1 cut(s)	45
BspT107I	GGYRCC	3 cut(s)	409, 874, 1026
BsrBI	CCGCTC	2 cut(s)	129, 178
BsrDI	GCAATG	1 cut(s)	1015
BsrFI	RCCGGY	1 cut(s)	620
BsrGI	TGTACA	1 cut(s)	666
BsrI	ACTGG	1 cut(s)	1028
BssAI	RCCGGY	1 cut(s)	620
BssECI	CCNNGG	5 cut(s)	91, 145, 293, 769, 1034
BssMI	GATC	5 cut(s)	208, 588, 679, 854, 997
BssNI	GRCGYC	1 cut(s)	582
BssT1I	CCWWGG	2 cut(s)	91, 145
Bst2UI	CCWGG	4 cut(s)	268, 295, 770, 1051
Bst4CI	ACNGT	2 cut(s)	666, 846
Bst6I	CTCTTC	1 cut(s)	45
BstACI	GRCGYC	1 cut(s)	582
BstAPI	GCANNNNNTGC	1 cut(s)	1014
BstAUI	TGTACA	1 cut(s)	666
BstC8I	GCNNGC	2 cut(s)	193, 1031
BstDEI	CTNAG	7 cut(s)	61, 213, 317, 362, 630, 829, 908
BstDSI	CCRYGG	1 cut(s)	91
BstF5I	GGATG	3 cut(s)	582, 976, 976
BstKTI	GATC	5 cut(s)	211, 591, 682, 857, 1000
BstMAI	GTCTC	1 cut(s)	633
BstMBI	GATC	5 cut(s)	208, 588, 679, 854, 997
BstMWI	GCNNNNNNNGC	5 cut(s)	77, 563, 1014, 1026, 1039
BstNI	CCWGG	4 cut(s)	268, 295, 770, 1051
BstNSI	RCATGY	2 cut(s)	376, 404
BstPAI	GACNNNNGTC	1 cut(s)	847
BstSCI	CCNGG	7 cut(s)	266, 293, 768, 924, 1033, 1034, 1049
BstSFI	CTRYAG	1 cut(s)	1015
BstSLI	GKGCMC	1 cut(s)	877
BstV1I	GCAGC	5 cut(s)	89, 119, 874, 1029, 1082
BstV2I	GAAGAC	3 cut(s)	46, 754, 827
BstX2I	RGATCY	1 cut(s)	208
BstYI	RGATCY	1 cut(s)	208
BsuRI	GGCC	1 cut(s)	528
BtgI	CCRYGG	1 cut(s)	91
BtsCI	GGATG	3 cut(s)	582, 976, 976
BtsIMutI	CAGTG	2 cut(s)	359, 1021
Cac8I	GCNNGC	2 cut(s)	193, 1031
CaiI	CAGNNNCTG	1 cut(s)	1023
Cfr10I	RCCGGY	1 cut(s)	620
Cfr13I	GGNCC	2 cut(s)	612, 842
Cfr9I	CCCGGG	1 cut(s)	1034
CseI	GACGC	1 cut(s)	393
Csp6I	GTAC	1 cut(s)	667
CviAII	CATG	7 cut(s)	92, 373, 401, 567, 676, 714, 1081
CviQI	GTAC	1 cut(s)	667
DdeI	CTNAG	7 cut(s)	61, 213, 317, 362, 630, 829, 908
DpnI	GATC	5 cut(s)	210, 590, 681, 856, 999
DpnII	GATC	5 cut(s)	208, 588, 679, 854, 997
EaeI	YGGCCR	1 cut(s)	526
Eam1104I	CTCTTC	1 cut(s)	45
EarI	CTCTTC	1 cut(s)	45
EciI	GGCGGA	1 cut(s)	178
Eco130I	CCWWGG	2 cut(s)	91, 145
Eco47I	GGWCC	2 cut(s)	612, 842
Eco88I	CYCGRG	1 cut(s)	1034
EcoRI	GAATTC	1 cut(s)	808
EcoRII	CCWGG	4 cut(s)	266, 293, 768, 1049
EcoT14I	CCWWGG	2 cut(s)	91, 145
EcoT22I	ATGCAT	1 cut(s)	568
ErhI	CCWWGG	2 cut(s)	91, 145
FaeI	CATG	7 cut(s)	95, 376, 404, 570, 679, 717, 1084
FalI	AAGNNNNNCTT	2 cut(s)	413, 445
FaqI	GGGAC	2 cut(s)	183, 670
FatI	CATG	7 cut(s)	91, 372, 400, 566, 675, 713, 1080
FauI	CCCGC	1 cut(s)	183
Fnu4HI	GCNGC	6 cut(s)	78, 130, 133, 888, 1018, 1071
FokI	GGATG	3 cut(s)	589, 963, 983
Fsp4HI	GCNGC	6 cut(s)	78, 130, 133, 888, 1018, 1071
FspBI	CTAG	1 cut(s)	720
GluI	GCNGC	6 cut(s)	78, 130, 133, 888, 1018, 1071
GsaI	CCCAGC	1 cut(s)	869
HaeIII	GGCC	1 cut(s)	528
HapII	CCGG	5 cut(s)	487, 558, 621, 926, 1035
HgaI	GACGC	1 cut(s)	393
Hin1I	GRCGYC	1 cut(s)	582
Hin1II	CATG	7 cut(s)	95, 376, 404, 570, 679, 717, 1084
HincII	GTYRAC	1 cut(s)	730
HindII	GTYRAC	1 cut(s)	730
HinfI	GANTC	7 cut(s)	108, 185, 278, 356, 364, 380, 731
HpaII	CCGG	5 cut(s)	487, 558, 621, 926, 1035
Hpy166II	GTNNAC	4 cut(s)	112, 612, 730, 931
Hpy188I	TCNGA	3 cut(s)	64, 318, 515
Hpy188III	TCNNGA	7 cut(s)	212, 487, 586, 650, 750, 831, 852
Hpy8I	GTNNAC	4 cut(s)	112, 612, 730, 931
Hpy99I	CGWCG	1 cut(s)	409
HpyAV	CCTTC	2 cut(s)	211, 439
HpyCH4III	ACNGT	2 cut(s)	666, 846
HpyCH4IV	ACGT	1 cut(s)	582
HpyCH4V	TGCA	5 cut(s)	135, 225, 566, 1017, 1070
HpyF10VI	GCNNNNNNNGC	5 cut(s)	77, 563, 1014, 1026, 1039
HpyF3I	CTNAG	7 cut(s)	61, 213, 317, 362, 630, 829, 908
HpySE526I	ACGT	1 cut(s)	582
Hsp92I	GRCGYC	1 cut(s)	582
Hsp92II	CATG	7 cut(s)	95, 376, 404, 570, 679, 717, 1084
Kpn2I	TCCGGA	1 cut(s)	486
Kzo9I	GATC	5 cut(s)	208, 588, 679, 854, 997
LguI	GCTCTTC	1 cut(s)	45
LmnI	GCTCC	1 cut(s)	18
Lsp1109I	GCAGC	5 cut(s)	89, 119, 874, 1029, 1082
LweI	GCATC	4 cut(s)	105, 154, 234, 961
MaeI	CTAG	1 cut(s)	720
MaeII	ACGT	1 cut(s)	582
MaeIII	GTNAC	2 cut(s)	284, 469
MalI	GATC	5 cut(s)	210, 590, 681, 856, 999
MbiI	CCGCTC	2 cut(s)	129, 178
MboI	GATC	5 cut(s)	208, 588, 679, 854, 997
MboII	GAAGA	7 cut(s)	32, 46, 49, 583, 754, 757, 832
MfeI	CAATTG	1 cut(s)	151
MflI	RGATCY	1 cut(s)	208
MhlI	GDGCHC	1 cut(s)	877
MluCI	AATT	3 cut(s)	136, 151, 808
MlyI	GAGTC	5 cut(s)	117, 272, 350, 373, 725
MmeI	TCCRAC	1 cut(s)	806
MnlI	CCTC	8 cut(s)	58, 266, 312, 315, 321, 451, 888, 991
Mph1103I	ATGCAT	1 cut(s)	568
MroI	TCCGGA	1 cut(s)	486
MseI	TTAA	1 cut(s)	918
MslI	CAYNNNNRTG	1 cut(s)	90
MspA1I	CMGCKG	1 cut(s)	195
MspI	CCGG	5 cut(s)	487, 558, 621, 926, 1035
MspR9I	CCNGG	7 cut(s)	268, 295, 770, 926, 1035, 1036, 1051
MunI	CAATTG	1 cut(s)	151
MvaI	CCWGG	4 cut(s)	268, 295, 770, 1051
MwoI	GCNNNNNNNGC	5 cut(s)	77, 563, 1014, 1026, 1039
NciI	CCSGG	3 cut(s)	926, 1035, 1036
NcoI	CCATGG	1 cut(s)	91
NdeII	GATC	5 cut(s)	208, 588, 679, 854, 997
NlaIII	CATG	7 cut(s)	95, 376, 404, 570, 679, 717, 1084
NlaIV	GGNNCC	5 cut(s)	411, 614, 876, 1028, 1055
NmuCI	GTSAC	1 cut(s)	469
NsiI	ATGCAT	1 cut(s)	568
NspI	RCATGY	2 cut(s)	376, 404
PciI	ACATGT	1 cut(s)	372
PciSI	GCTCTTC	1 cut(s)	45
PfeI	GAWTC	2 cut(s)	185, 380
PkrI	GCNGC	6 cut(s)	79, 131, 134, 889, 1019, 1072
PleI	GAGTC	5 cut(s)	116, 272, 350, 372, 725
PpsI	GAGTC	5 cut(s)	116, 272, 350, 372, 725
PscI	ACATGT	1 cut(s)	372
PshAI	GACNNNNGTC	1 cut(s)	847
PsiI	TTATAA	1 cut(s)	699
Psp6I	CCWGG	4 cut(s)	266, 293, 768, 1049
PspFI	CCCAGC	1 cut(s)	865
PspGI	CCWGG	4 cut(s)	266, 293, 768, 1049
PspN4I	GGNNCC	5 cut(s)	411, 614, 876, 1028, 1055
PspPI	GGNCC	2 cut(s)	612, 842
PsrI	GAACNNNNNNTAC	2 cut(s)	914, 946
PstI	CTGCAG	1 cut(s)	1019
PstNI	CAGNNNCTG	1 cut(s)	1023
PsuI	RGATCY	1 cut(s)	208
PvuII	CAGCTG	1 cut(s)	195
RsaI	GTAC	1 cut(s)	668
RsaNI	GTAC	1 cut(s)	667
RseI	CAYNNNNRTG	1 cut(s)	90
SapI	GCTCTTC	1 cut(s)	45
SaqAI	TTAA	1 cut(s)	918
SatI	GCNGC	6 cut(s)	78, 130, 133, 888, 1018, 1071
Sau3AI	GATC	5 cut(s)	208, 588, 679, 854, 997
Sau96I	GGNCC	2 cut(s)	612, 842
SchI	GAGTC	5 cut(s)	117, 272, 350, 373, 725
ScrFI	CCNGG	7 cut(s)	268, 295, 770, 926, 1035, 1036, 1051
SduI	GDGCHC	1 cut(s)	877
SfaNI	GCATC	4 cut(s)	105, 154, 234, 961
SfcI	CTRYAG	1 cut(s)	1015
SinI	GGWCC	2 cut(s)	612, 842
SmaI	CCCGGG	1 cut(s)	1036
SmiMI	CAYNNNNRTG	1 cut(s)	90
SmlI	CTYRAG	2 cut(s)	103, 257
SmoI	CTYRAG	2 cut(s)	103, 257
Sse9I	AATT	3 cut(s)	136, 151, 808
SsiI	CCGC	3 cut(s)	129, 176, 189
SspMI	CTAG	1 cut(s)	720
StyD4I	CCNGG	7 cut(s)	266, 293, 768, 924, 1033, 1034, 1049
StyI	CCWWGG	2 cut(s)	91, 145
TaaI	ACNGT	2 cut(s)	666, 846
TaiI	ACGT	1 cut(s)	585
TaqI	TCGA	1 cut(s)	939
TasI	AATT	3 cut(s)	136, 151, 808
TatI	WGTACW	1 cut(s)	666
TauI	GCSGC	1 cut(s)	132
TfiI	GAWTC	2 cut(s)	185, 380
Tru1I	TTAA	1 cut(s)	918
Tru9I	TTAA	1 cut(s)	918
TscAI	CASTG	2 cut(s)	366, 1028
TseFI	GTSAC	1 cut(s)	469
TseI	GCWGC	5 cut(s)	77, 132, 887, 1017, 1070
Tsp45I	GTSAC	1 cut(s)	469
TspDTI	ATGAA	1 cut(s)	193
TspMI	CCCGGG	1 cut(s)	1034
TspRI	CASTG	2 cut(s)	366, 1028
VpaK11BI	GGWCC	2 cut(s)	612, 842
XapI	RAATTY	1 cut(s)	808
XceI	RCATGY	2 cut(s)	376, 404
XmaI	CCCGGG	1 cut(s)	1034
XspI	CTAG	1 cut(s)	720
ZraI	GACGTC	1 cut(s)	583
Zsp2I	ATGCAT	1 cut(s)	568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.