AT2G35300

late embryogenesis abundant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011487
Physical Location & Seq
Reverse (-)
14862779 .. 14863412
634 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35300.1

Sequence Viewer

Length: 294 bp
ATGCAGTCGGCGAAGGAAAAGATCAGTGACATGGCCAGTACGGCCAAGGAGAAACTCAACATCGGTGGCGCAAAGGCACAAGGTCATGCGGAGAAGACGATGGCAAGGACCAAAAAAGAGAAGAAGTTGGCCCAAGAGCGAGAGAAGTCTAAGGAGGCGCAGGCCAAAGCTGACCTCCATCAATCCAAGGCTGAGCATGCTGCGGACGCTCAGGTTCACGGCCACCATCTTCCCGGTCACTCCACCTACCCTACCCGAGCCACCGGAGCTAATTACCCGCCGGGACAGATCTAA

Protein Analysis

97

Amino Acids

10.48

Weight (kDa)

9.66

Isoelectric Point (pI)

25.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 71 1.7e-23 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 89, 203, 278
AcoI YGGCCR 3 cut(s) 33, 42, 220
AfaI GTAC 1 cut(s) 40
AluBI AGCT 2 cut(s) 170, 269
AluI AGCT 2 cut(s) 170, 269
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 5 cut(s) 33, 42, 129, 162, 220
ApeKI GCWGC 1 cut(s) 200
AspLEI GCGC 2 cut(s) 71, 160
AspS9I GGNCC 2 cut(s) 108, 130
AsuC2I CCSGG 2 cut(s) 234, 282
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 1 cut(s) 108
BalI TGGCCA 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 187
BccI CCATC 3 cut(s) 94, 186, 234
BceAI ACGGC 2 cut(s) 57, 235
BcnI CCSGG 2 cut(s) 234, 282
BglI GCCNNNNNGGC 1 cut(s) 41
BglII AGATCT 1 cut(s) 288
BisI GCNGC 1 cut(s) 201
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 234, 282
Bme18I GGWCC 1 cut(s) 108
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 108, 130
BmrFI CCNGG 2 cut(s) 234, 282
BpiI GAAGAC 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 210
Bpu1102I GCTNAGC 1 cut(s) 192
BpuMI CCSGG 2 cut(s) 234, 282
BsaJI CCNNGG 2 cut(s) 45, 186
BsaWI WCCGGW 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 45, 186
BseMII CTCAG 2 cut(s) 183, 224
BseNI ACTGG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 187
BshFI GGCC 5 cut(s) 35, 44, 131, 164, 222
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 3 cut(s) 234, 264, 281
BsnI GGCC 5 cut(s) 35, 44, 131, 164, 222
BsoBI CYCGRG 1 cut(s) 255
Bsp143I GATC 2 cut(s) 21, 288
Bsp1720I GCTNAGC 1 cut(s) 192
BspACI CCGC 3 cut(s) 89, 203, 278
BspANI GGCC 5 cut(s) 35, 44, 131, 164, 222
BspCNI CTCAG 2 cut(s) 184, 223
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 45, 186
BssMI GATC 2 cut(s) 21, 288
BssT1I CCWWGG 2 cut(s) 45, 186
BstC8I GCNNGC 2 cut(s) 162, 198
BstDEI CTNAG 3 cut(s) 150, 192, 210
BstHHI GCGC 2 cut(s) 71, 160
BstKTI GATC 2 cut(s) 24, 291
BstMBI GATC 2 cut(s) 21, 288
BstMWI GCNNNNNNNGC 4 cut(s) 41, 197, 206, 266
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 232, 280
BstV1I GCAGC 1 cut(s) 187
BstV2I GAAGAC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BsuRI GGCC 5 cut(s) 35, 44, 131, 164, 222
BtsIMutI CAGTG 1 cut(s) 31
Cac8I GCNNGC 2 cut(s) 162, 198
CfoI GCGC 2 cut(s) 71, 160
Cfr13I GGNCC 2 cut(s) 108, 130
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 39
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 3 cut(s) 31, 86, 197
CviJI RGCY 9 cut(s) 35, 44, 131, 164, 170, 191, 222, 260, 269
CviKI_1 RGCY 9 cut(s) 35, 44, 131, 164, 170, 191, 222, 260, 269
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 3 cut(s) 150, 192, 210
DpnI GATC 2 cut(s) 23, 290
DpnII GATC 2 cut(s) 21, 288
EaeI YGGCCR 3 cut(s) 33, 42, 220
Eco130I CCWWGG 2 cut(s) 45, 186
Eco47I GGWCC 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 255
EcoT14I CCWWGG 2 cut(s) 45, 186
ErhI CCWWGG 2 cut(s) 45, 186
FaeI CATG 3 cut(s) 34, 89, 200
FaiI YATR 3 cut(s) 32, 87, 198
FatI CATG 3 cut(s) 30, 85, 196
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 201
GlaI GCGC 2 cut(s) 70, 159
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 5 cut(s) 35, 44, 131, 164, 222
HapII CCGG 3 cut(s) 234, 264, 281
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 71, 160
Hin1II CATG 3 cut(s) 34, 89, 200
Hin6I GCGC 2 cut(s) 69, 158
HinP1I GCGC 2 cut(s) 69, 158
HpaII CCGG 3 cut(s) 234, 264, 281
Hpy166II GTNNAC 1 cut(s) 217
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 1 cut(s) 7
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 197, 206, 266
HpyF3I CTNAG 3 cut(s) 150, 192, 210
Hsp92II CATG 3 cut(s) 34, 89, 200
HspAI GCGC 2 cut(s) 69, 158
Kzo9I GATC 2 cut(s) 21, 288
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 5 cut(s) 49, 146, 197, 247, 277
Lsp1109I GCAGC 1 cut(s) 187
MaeIII GTNAC 2 cut(s) 26, 236
MalI GATC 2 cut(s) 23, 290
MboI GATC 2 cut(s) 21, 288
MboII GAAGA 3 cut(s) 106, 133, 221
MflI RGATCY 1 cut(s) 288
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 1 cut(s) 271
MluNI TGGCCA 1 cut(s) 35
MnlI CCTC 2 cut(s) 148, 185
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 35
MspI CCGG 3 cut(s) 234, 264, 281
MspR9I CCNGG 2 cut(s) 234, 282
MwoI GCNNNNNNNGC 4 cut(s) 41, 197, 206, 266
NciI CCSGG 2 cut(s) 234, 282
NdeII GATC 2 cut(s) 21, 288
NlaIII CATG 3 cut(s) 34, 89, 200
NmuCI GTSAC 2 cut(s) 26, 236
NspI RCATGY 1 cut(s) 200
PaeI GCATGC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 202
PspPI GGNCC 2 cut(s) 108, 130
PsuI RGATCY 1 cut(s) 288
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 2 cut(s) 21, 288
Sau96I GGNCC 2 cut(s) 108, 130
ScrFI CCNGG 2 cut(s) 234, 282
SetI ASST 6 cut(s) 85, 172, 177, 216, 248, 271
SfiI GGCCNNNNNGGCC 1 cut(s) 41
SinI GGWCC 1 cut(s) 108
SphI GCATGC 1 cut(s) 200
Sse9I AATT 1 cut(s) 271
SsiI CCGC 3 cut(s) 89, 203, 278
StyD4I CCNGG 2 cut(s) 232, 280
StyI CCWWGG 2 cut(s) 45, 186
TasI AATT 1 cut(s) 271
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 2 cut(s) 26, 236
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 2 cut(s) 26, 236
TspRI CASTG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 108
XceI RCATGY 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.