Rroxscaffold_3G00249510

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011487
Physical Location & Seq
Reverse (-)
43278383 .. 43279297
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00249510.1

Sequence Viewer

Length: 312 bp
ATGCAGTCTGCAAAGGAGAAGATCAGTAACTTGGCCAGTGCTGCCAAGGAGGAAGTAGAGACTCACAAAGCCAGAACGGAAGAGAAGGTTGAGAAGATGACAGCGAGGACAGAGGAAGAGAAGAGAATAGCGGAGGAGAAGAGAAAAGCTAAAGAGGCAGAGGCGAAGATGAAGCTGCATGAGTCCAAAGCCGAGCATGAAGCCGATAAACTGACTACTGGTGCTCCTGCAACTGATGCCGGTGGTCCAATGGGTAGGACCGCAGCTCCATCGTATCCCCCGGGAGGTTACAGCACCCGAAAACTTCCCTAG

Protein Analysis

103

Amino Acids

11.25

Weight (kDa)

8.8

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 8.9e-23 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 131, 261
AcoI YGGCCR 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 284
AluBI AGCT 3 cut(s) 149, 175, 266
AluI AGCT 3 cut(s) 149, 175, 266
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 3 cut(s) 41, 175, 263
AspS9I GGNCC 2 cut(s) 245, 258
AsuC2I CCSGG 2 cut(s) 281, 282
AvaI CYCGRG 1 cut(s) 280
AvaII GGWCC 2 cut(s) 245, 258
BalI TGGCCA 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 3 cut(s) 28, 162, 275
BccI CCATC 1 cut(s) 277
BcgI CGANNNNNNTGC 2 cut(s) 252, 286
BciVI GTATCC 1 cut(s) 285
BcnI CCSGG 2 cut(s) 281, 282
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 1 cut(s) 310
BfuI GTATCC 1 cut(s) 285
BisI GCNGC 3 cut(s) 42, 176, 264
BlsI GCNGC 3 cut(s) 43, 177, 265
Bme1390I CCNGG 2 cut(s) 281, 282
Bme18I GGWCC 2 cut(s) 245, 258
BmeT110I CYCGRG 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 245, 258
BmrFI CCNGG 2 cut(s) 281, 282
BmsI GCATC 1 cut(s) 226
BpuMI CCSGG 2 cut(s) 281, 282
BsaJI CCNNGG 3 cut(s) 45, 279, 280
BsaXI ACNNNNNCTCC 4 cut(s) 208, 238, 250, 280
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 2 cut(s) 36, 223
BseDI CCNNGG 3 cut(s) 45, 279, 280
BseLI CCNNNNNNNGG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 36, 223
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 3 cut(s) 28, 162, 275
BshFI GGCC 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 2 cut(s) 240, 281
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 53
BsnI GGCC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 226
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 2 cut(s) 131, 261
BspANI GGCC 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 2 cut(s) 36, 223
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 3 cut(s) 45, 279, 280
BssMI GATC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 45
Bst6I CTCTTC 4 cut(s) 75, 111, 116, 134
BstAPI GCANNNNNTGC 1 cut(s) 236
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 3 cut(s) 41, 155, 236
BstSCI CCNGG 2 cut(s) 279, 280
BstV1I GCAGC 3 cut(s) 28, 162, 275
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 2 cut(s) 245, 258
Cfr9I CCCGGG 1 cut(s) 280
CviAII CATG 2 cut(s) 179, 197
CviJI RGCY 7 cut(s) 35, 71, 149, 175, 191, 203, 266
CviKI_1 RGCY 7 cut(s) 35, 71, 149, 175, 191, 203, 266
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 4 cut(s) 75, 111, 116, 134
EarI CTCTTC 4 cut(s) 75, 111, 116, 134
Eco130I CCWWGG 1 cut(s) 45
Eco47I GGWCC 2 cut(s) 245, 258
Eco88I CYCGRG 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 182, 200
FaiI YATR 2 cut(s) 180, 198
FatI CATG 2 cut(s) 178, 196
Fnu4HI GCNGC 3 cut(s) 42, 176, 264
Fsp4HI GCNGC 3 cut(s) 42, 176, 264
FspBI CTAG 1 cut(s) 310
GluI GCNGC 3 cut(s) 42, 176, 264
HaeIII GGCC 1 cut(s) 35
HapII CCGG 2 cut(s) 240, 281
Hin1II CATG 2 cut(s) 182, 200
HinfI GANTC 2 cut(s) 61, 182
HpaII CCGG 2 cut(s) 240, 281
HpyAV CCTTC 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 4, 11, 178, 230
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 155, 236
Hsp92II CATG 2 cut(s) 182, 200
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 229, 271
LpnPI CCDG 6 cut(s) 49, 85, 204, 240, 253, 294
Lsp1109I GCAGC 3 cut(s) 28, 162, 275
LweI GCATC 1 cut(s) 226
MaeI CTAG 1 cut(s) 310
MaeIII GTNAC 2 cut(s) 26, 287
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 7 cut(s) 31, 92, 106, 128, 133, 151, 178
MhlI GDGCHC 1 cut(s) 226
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 2 cut(s) 55, 191
MnlI CCTC 7 cut(s) 43, 99, 106, 127, 148, 154, 278
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 35
MspI CCGG 2 cut(s) 240, 281
MspR9I CCNGG 2 cut(s) 281, 282
MwoI GCNNNNNNNGC 3 cut(s) 41, 155, 236
NciI CCSGG 2 cut(s) 281, 282
NdeII GATC 1 cut(s) 21
NlaIII CATG 2 cut(s) 182, 200
NmeAIII GCCGAG 1 cut(s) 217
PkrI GCNGC 3 cut(s) 43, 177, 265
PleI GAGTC 2 cut(s) 55, 190
PpsI GAGTC 2 cut(s) 55, 190
PspPI GGNCC 2 cut(s) 245, 258
SatI GCNGC 3 cut(s) 42, 176, 264
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 2 cut(s) 245, 258
SchI GAGTC 2 cut(s) 55, 191
ScrFI CCNGG 2 cut(s) 281, 282
SduI GDGCHC 1 cut(s) 226
SetI ASST 5 cut(s) 90, 151, 177, 268, 289
SfaNI GCATC 1 cut(s) 226
SinI GGWCC 2 cut(s) 245, 258
SmaI CCCGGG 1 cut(s) 282
SsiI CCGC 2 cut(s) 131, 261
SspMI CTAG 1 cut(s) 310
StyD4I CCNGG 2 cut(s) 279, 280
StyI CCWWGG 1 cut(s) 45
TscAI CASTG 1 cut(s) 43
TseI GCWGC 3 cut(s) 41, 175, 263
TspDTI ATGAA 2 cut(s) 185, 213
TspGWI ACGGA 1 cut(s) 92
TspMI CCCGGG 1 cut(s) 280
TspRI CASTG 1 cut(s) 43
VpaK11BI GGWCC 2 cut(s) 245, 258
XmaI CCCGGG 1 cut(s) 280
XspI CTAG 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.