FvH4_5g21820

Late embryogenesis abundant (LEA) group 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011487
Physical Location & Seq
Forward (+)
13195053 .. 13195832
780 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21820.t1

Sequence Viewer

Length: 303 bp
ATGCAGTCTGGAAAGGAGAAGATGAGCAACATGGCCAGTGCTGCCAAGGAAAAAGCAGAGGTTTACAAATCCAAAGCGGAAGAGAAGGTTGAGAAGATGACAACGAGTACAGAGGAAGAAAAAAGAATAGCGGAGGAGAAGAGAGGAGCCAAAGAAGCAGAAGCAAAAATGAAGCTGCATGAATCGAAAGCAGAGCATGCAGCCGGTAAATTGGCTACTGCTGGCCCTGCAACTGGTGCTCCGATAGGGACTGGAGTTCCGGCATATCCCCTGGGAGGTTACAGCACCCGGAAACTGCCCTAG

Protein Analysis

101

Amino Acids

10.64

Weight (kDa)

9.27

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 3.9e-22 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 77, 131
AcoI YGGCCR 1 cut(s) 33
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 2 cut(s) 233, 275
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
Alw21I GWGCWC 1 cut(s) 241
AoxI GGCC 2 cut(s) 33, 223
ApeKI GCWGC 3 cut(s) 41, 175, 200
AspS9I GGNCC 1 cut(s) 224
AsuC2I CCSGG 1 cut(s) 289
BalI TGGCCA 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 3 cut(s) 28, 162, 212
BciT130I CCWGG 1 cut(s) 272
BcnI CCSGG 1 cut(s) 289
BfaI CTAG 1 cut(s) 301
BisI GCNGC 3 cut(s) 42, 176, 201
BlsI GCNGC 3 cut(s) 43, 177, 202
Bme1390I CCNGG 2 cut(s) 272, 289
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 2 cut(s) 272, 289
BpmI CTGGAG 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 289
BsaJI CCNNGG 3 cut(s) 45, 270, 271
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 275
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 3 cut(s) 36, 238, 256
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 3 cut(s) 45, 270, 271
BseLI CCNNNNNNNGG 2 cut(s) 233, 275
BseNI ACTGG 3 cut(s) 36, 238, 256
BseRI GAGGAG 2 cut(s) 149, 159
BseXI GCAGC 3 cut(s) 28, 162, 212
BshFI GGCC 2 cut(s) 35, 225
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 3 cut(s) 204, 260, 289
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 2 cut(s) 233, 275
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 2 cut(s) 35, 225
Bsp1286I GDGCHC 1 cut(s) 241
BspACI CCGC 2 cut(s) 77, 131
BspANI GGCC 2 cut(s) 35, 225
BspLI GGNNCC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 3 cut(s) 36, 238, 256
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 3 cut(s) 45, 270, 271
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 272
Bst6I CTCTTC 2 cut(s) 75, 134
BstAPI GCANNNNNTGC 2 cut(s) 197, 236
BstC8I GCNNGC 2 cut(s) 198, 223
BstMWI GCNNNNNNNGC 5 cut(s) 41, 155, 197, 227, 236
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 270, 287
BstV1I GCAGC 3 cut(s) 28, 162, 212
BsuRI GGCC 2 cut(s) 35, 225
BtsIMutI CAGTG 1 cut(s) 43
Cac8I GCNNGC 2 cut(s) 198, 223
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 108
CviAII CATG 3 cut(s) 31, 179, 197
CviJI RGCY 6 cut(s) 35, 149, 175, 203, 215, 225
CviKI_1 RGCY 6 cut(s) 35, 149, 175, 203, 215, 225
CviQI GTAC 1 cut(s) 108
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 2 cut(s) 75, 134
EarI CTCTTC 2 cut(s) 75, 134
Eco130I CCWWGG 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 3 cut(s) 34, 182, 200
FaiI YATR 4 cut(s) 32, 180, 198, 265
FaqI GGGAC 1 cut(s) 262
FatI CATG 3 cut(s) 30, 178, 196
Fnu4HI GCNGC 3 cut(s) 42, 176, 201
Fsp4HI GCNGC 3 cut(s) 42, 176, 201
FspBI CTAG 1 cut(s) 301
GluI GCNGC 3 cut(s) 42, 176, 201
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 2 cut(s) 35, 225
HapII CCGG 3 cut(s) 204, 260, 289
Hin1II CATG 3 cut(s) 34, 182, 200
HinfI GANTC 1 cut(s) 182
HpaII CCGG 3 cut(s) 204, 260, 289
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 1 cut(s) 9
Hpy8I GTNNAC 1 cut(s) 64
HpyAV CCTTC 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 4, 178, 200, 230
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 155, 197, 227, 236
Hsp92II CATG 3 cut(s) 34, 182, 200
LmnI GCTCC 2 cut(s) 146, 244
LpnPI CCDG 9 cut(s) 49, 207, 217, 219, 237, 240, 257, 273, 284
Lsp1109I GCAGC 3 cut(s) 28, 162, 212
MaeI CTAG 1 cut(s) 301
MaeIII GTNAC 1 cut(s) 278
MboII GAAGA 5 cut(s) 31, 92, 106, 128, 151
MhlI GDGCHC 1 cut(s) 241
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 35
MnlI CCTC 5 cut(s) 52, 106, 127, 137, 269
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 35
MspI CCGG 3 cut(s) 204, 260, 289
MspR9I CCNGG 2 cut(s) 272, 289
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 5 cut(s) 41, 155, 197, 227, 236
NciI CCSGG 1 cut(s) 289
NlaIII CATG 3 cut(s) 34, 182, 200
NlaIV GGNNCC 1 cut(s) 148
NspI RCATGY 1 cut(s) 200
PaeI GCATGC 1 cut(s) 200
PasI CCCWGGG 1 cut(s) 271
PfeI GAWTC 1 cut(s) 182
PkrI GCNGC 3 cut(s) 43, 177, 202
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 224
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SatI GCNGC 3 cut(s) 42, 176, 201
Sau96I GGNCC 1 cut(s) 224
ScrFI CCNGG 2 cut(s) 272, 289
SduI GDGCHC 1 cut(s) 241
SetI ASST 4 cut(s) 63, 90, 177, 280
SphI GCATGC 1 cut(s) 200
Sse9I AATT 1 cut(s) 209
SsiI CCGC 2 cut(s) 77, 131
SspMI CTAG 1 cut(s) 301
StyD4I CCNGG 2 cut(s) 270, 287
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 1 cut(s) 185
TasI AATT 1 cut(s) 209
TatI WGTACW 1 cut(s) 107
TfiI GAWTC 1 cut(s) 182
TscAI CASTG 1 cut(s) 43
TseI GCWGC 3 cut(s) 41, 175, 200
TspDTI ATGAA 2 cut(s) 185, 195
TspRI CASTG 1 cut(s) 43
XceI RCATGY 1 cut(s) 200
XspI CTAG 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.