Rh7DG252300

Late embryogenesis abundant (LEA) group 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011487
Physical Location & Seq
Forward (+)
26175533 .. 26176008
476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG252300.1

Sequence Viewer

Length: 360 bp
ATGCAGGCCGCAAAGGAAAAGCTTAGCAATCTGGCCAGCTCGGCCAAGGAGCACGTCAATATCTACAAAGCCAAAGTAGAAGAAAAGTTGGAGAGAGCAAAAGCTAGGAATAAGGAAGAGAAGAAGATGGCGAGAGAGCGTCGGAAAGTGAAGGAGGCCAGTGCAAAGATGGAGCTGCATAAAGCTAAAGCCAGGCATGCAGAGAAGAAATTAGGTGCCAAACTATCTCATGGGCATGGGATATTCAACCATCACCACCCACCACCAGCACCACTTCATAATCATCAACATCAACATCAACATGGGCACCAGCATCTGGGAACAACTAATATGATCCCAGCTTCAAATCCGCGTGTGTAG

Protein Analysis

119

Amino Acids

13.54

Weight (kDa)

10.32

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 1e-22 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 215, 306
AccB7I CCANNNNNTGG 1 cut(s) 316
AccII CGCG 1 cut(s) 352
AciI CCGC 2 cut(s) 9, 350
AclWI GGATC 1 cut(s) 328
AcoI YGGCCR 2 cut(s) 33, 42
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 2 cut(s) 247, 345
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 1 cut(s) 191
AluBI AGCT 6 cut(s) 22, 39, 104, 175, 185, 341
AluI AGCT 6 cut(s) 22, 39, 104, 175, 185, 341
Alw21I GWGCWC 1 cut(s) 54
AlwI GGATC 1 cut(s) 328
AlwNI CAGNNNCTG 1 cut(s) 316
AoxI GGCC 4 cut(s) 6, 33, 42, 156
ApeKI GCWGC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 309
BalI TGGCCA 1 cut(s) 35
BanI GGYRCC 2 cut(s) 215, 306
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 162
BccI CCATC 3 cut(s) 121, 163, 258
BciT130I CCWGG 1 cut(s) 193
BfaI CTAG 1 cut(s) 105
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 2 cut(s) 9, 176
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 2 cut(s) 10, 177
Bme1390I CCNGG 1 cut(s) 193
BmgBI CACGTC 1 cut(s) 55
BmiI GGNNCC 2 cut(s) 217, 308
BmrFI CCNGG 1 cut(s) 193
BmsI GCATC 1 cut(s) 322
Bpu1102I GCTNAGC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 159
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 45
BseLI CCNNNNNNNGG 1 cut(s) 316
BseNI ACTGG 1 cut(s) 159
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 1 cut(s) 162
BseYI CCCAGC 1 cut(s) 337
Bsh1236I CGCG 1 cut(s) 352
BshFI GGCC 4 cut(s) 8, 35, 44, 158
BshNI GGYRCC 2 cut(s) 215, 306
BsiHKAI GWGCWC 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 316
BsnI GGCC 4 cut(s) 8, 35, 44, 158
Bsp1286I GDGCHC 2 cut(s) 54, 309
Bsp143I GATC 1 cut(s) 333
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 2 cut(s) 9, 350
BspANI GGCC 4 cut(s) 8, 35, 44, 158
BspFNI CGCG 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 217, 308
BspPI GGATC 1 cut(s) 328
BspT107I GGYRCC 2 cut(s) 215, 306
BsrI ACTGG 1 cut(s) 159
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 193
Bst6I CTCTTC 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 6, 37, 198
BstDEI CTNAG 1 cut(s) 23
BstFNI CGCG 1 cut(s) 352
BstKTI GATC 1 cut(s) 336
BstMBI GATC 1 cut(s) 333
BstMWI GCNNNNNNNGC 2 cut(s) 41, 197
BstNI CCWGG 1 cut(s) 193
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 191
BstSLI GKGCMC 1 cut(s) 309
BstUI CGCG 1 cut(s) 352
BstV1I GCAGC 1 cut(s) 162
BsuRI GGCC 4 cut(s) 8, 35, 44, 158
BtrI CACGTC 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 3 cut(s) 6, 37, 198
CaiI CAGNNNCTG 1 cut(s) 316
CseI GACGC 1 cut(s) 128
CviAII CATG 4 cut(s) 197, 230, 236, 302
DdeI CTNAG 1 cut(s) 23
DpnI GATC 1 cut(s) 335
DpnII GATC 1 cut(s) 333
EaeI YGGCCR 2 cut(s) 33, 42
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 4 cut(s) 200, 233, 239, 305
FaiI YATR 7 cut(s) 180, 198, 231, 237, 279, 303, 332
FatI CATG 4 cut(s) 196, 229, 235, 301
Fnu4HI GCNGC 2 cut(s) 9, 176
Fsp4HI GCNGC 2 cut(s) 9, 176
FspBI CTAG 1 cut(s) 105
GluI GCNGC 2 cut(s) 9, 176
GsaI CCCAGC 1 cut(s) 341
HaeIII GGCC 4 cut(s) 8, 35, 44, 158
HgaI GACGC 1 cut(s) 128
Hin1II CATG 4 cut(s) 200, 233, 239, 305
HindIII AAGCTT 1 cut(s) 20
HphI GGTGA 1 cut(s) 245
Hpy188I TCNGA 1 cut(s) 144
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 4 cut(s) 4, 164, 178, 200
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 197
HpyF3I CTNAG 1 cut(s) 23
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 4 cut(s) 200, 233, 239, 305
Kzo9I GATC 1 cut(s) 333
LmnI GCTCC 2 cut(s) 49, 172
LpnPI CCDG 9 cut(s) 17, 49, 172, 178, 205, 279, 302, 323, 351
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 105
MaeII ACGT 1 cut(s) 54
MalI GATC 1 cut(s) 335
MboI GATC 1 cut(s) 333
MboII GAAGA 5 cut(s) 92, 128, 133, 136, 217
MhlI GDGCHC 2 cut(s) 54, 309
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 35
MmeI TCCRAC 2 cut(s) 69, 122
MnlI CCTC 1 cut(s) 148
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MslI CAYNNNNRTG 2 cut(s) 234, 300
Msp20I TGGCCA 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 193
MvaI CCWGG 1 cut(s) 193
MvnI CGCG 1 cut(s) 352
MwoI GCNNNNNNNGC 2 cut(s) 41, 197
NdeII GATC 1 cut(s) 333
NlaIII CATG 4 cut(s) 200, 233, 239, 305
NlaIV GGNNCC 2 cut(s) 217, 308
NmeAIII GCCGAG 1 cut(s) 20
NspI RCATGY 1 cut(s) 200
PaeI GCATGC 1 cut(s) 200
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 2 cut(s) 10, 177
Psp6I CCWGG 1 cut(s) 191
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 2 cut(s) 217, 308
PstNI CAGNNNCTG 1 cut(s) 316
RseI CAYNNNNRTG 2 cut(s) 234, 300
SatI GCNGC 2 cut(s) 9, 176
Sau3AI GATC 1 cut(s) 333
ScrFI CCNGG 1 cut(s) 193
SduI GDGCHC 2 cut(s) 54, 309
SetI ASST 8 cut(s) 24, 41, 57, 106, 177, 187, 217, 343
SfaNI GCATC 1 cut(s) 322
SfiI GGCCNNNNNGGCC 1 cut(s) 41
SmiMI CAYNNNNRTG 2 cut(s) 234, 300
SphI GCATGC 1 cut(s) 200
Sse9I AATT 1 cut(s) 209
SsiI CCGC 2 cut(s) 9, 350
SspMI CTAG 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 191
StyI CCWWGG 1 cut(s) 45
TaiI ACGT 1 cut(s) 57
TasI AATT 1 cut(s) 209
TauI GCSGC 1 cut(s) 11
TscAI CASTG 1 cut(s) 166
TseI GCWGC 1 cut(s) 175
TspDTI ATGAA 1 cut(s) 266
TspRI CASTG 1 cut(s) 166
Van91I CCANNNNNTGG 1 cut(s) 316
XceI RCATGY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XspI CTAG 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.