MD06G1032700.v1.1

Late embryogenesis abundant (LEA) group 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011487
Physical Location & Seq
Forward (+)
3915723 .. 3916791
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1032700.v1.1.491

Sequence Viewer

Length: 396 bp
ATGCAGTCTGCAAAGGAGAAGCTAAGCAATATGGCCAGTGCTGCCAAGGAGCACGTAGACATATACAAAGCTAAAGCTCAAGAGAAGGTGGTGAAGGCGACAGCAAAAACGGACGAAGAGAGGGAAATAGCGAGCCAGATAAGAAGAGCGAAAGAAGCAAAAGCAAAGATGGAGTTTCATGAGGCCAAAACCAAGCACTCTGCTGAAAAATCGACTGCTAGTGCAGAGAATAAATCCCATCTCCTCCATGGACAGGATGATCAGCCTGTGGCTAGGGTTGGTGATGGCCATGGTAATGATCACCAACCTCACGATGCGGGTCCTCTGCCTAAAACCACAGTTCCACAGTTCCGGCATACCCACTTGGAGGTTATCGCACCCACAAGTTTCCCTTGA

Protein Analysis

132

Amino Acids

14.43

Weight (kDa)

8.81

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 1.1e-21 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 57
AciI CCGC 1 cut(s) 317
AcoI YGGCCR 2 cut(s) 33, 286
AfiI CCNNNNNNNGG 2 cut(s) 253, 367
AluBI AGCT 3 cut(s) 22, 71, 77
AluI AGCT 3 cut(s) 22, 71, 77
Alw21I GWGCWC 1 cut(s) 54
AoxI GGCC 3 cut(s) 33, 183, 286
ApeKI GCWGC 1 cut(s) 41
AspS9I GGNCC 1 cut(s) 320
AsuHPI GGTGA 3 cut(s) 103, 293, 293
AvaII GGWCC 1 cut(s) 320
BalI TGGCCA 2 cut(s) 35, 288
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 28
BccI CCATC 3 cut(s) 163, 246, 278
BclI TGATCA 2 cut(s) 259, 298
BfaI CTAG 2 cut(s) 219, 273
BisI GCNGC 1 cut(s) 42
BlpI GCTNAGC 1 cut(s) 23
BlsI GCNGC 1 cut(s) 43
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 321
BmsI GCATC 1 cut(s) 304
Bpu1102I GCTNAGC 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 3 cut(s) 45, 247, 289
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 367
Bse1I ACTGG 1 cut(s) 36
BseDI CCNNGG 3 cut(s) 45, 247, 289
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 2 cut(s) 253, 367
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 1 cut(s) 233
BseXI GCAGC 1 cut(s) 28
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 3 cut(s) 35, 185, 288
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 1 cut(s) 352
BslI CCNNNNNNNGG 2 cut(s) 253, 367
BsnI GGCC 3 cut(s) 35, 185, 288
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 2 cut(s) 259, 298
Bsp1720I GCTNAGC 1 cut(s) 23
Bsp19I CCATGG 2 cut(s) 247, 289
BspACI CCGC 1 cut(s) 317
BspANI GGCC 3 cut(s) 35, 185, 288
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 321
BspQI GCTCTTC 1 cut(s) 139
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 3 cut(s) 45, 247, 289
BssMI GATC 2 cut(s) 259, 298
BssT1I CCWWGG 3 cut(s) 45, 247, 289
Bst4CI ACNGT 2 cut(s) 340, 348
Bst6I CTCTTC 2 cut(s) 111, 139
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 133
BstDEI CTNAG 1 cut(s) 23
BstDSI CCRYGG 2 cut(s) 247, 289
BstF5I GGATG 1 cut(s) 262
BstKTI GATC 2 cut(s) 262, 301
BstMBI GATC 2 cut(s) 259, 298
BstMWI GCNNNNNNNGC 2 cut(s) 41, 155
BstV1I GCAGC 1 cut(s) 28
BsuRI GGCC 3 cut(s) 35, 185, 288
BtgI CCRYGG 2 cut(s) 247, 289
BtsCI GGATG 1 cut(s) 262
BtsIMutI CAGTG 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 133
CciI TCATGA 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 320
CviAII CATG 3 cut(s) 179, 248, 290
CviJI RGCY 9 cut(s) 22, 35, 71, 77, 135, 185, 265, 272, 288
CviKI_1 RGCY 9 cut(s) 22, 35, 71, 77, 135, 185, 265, 272, 288
DdeI CTNAG 1 cut(s) 23
DpnI GATC 2 cut(s) 261, 300
DpnII GATC 2 cut(s) 259, 298
EaeI YGGCCR 2 cut(s) 33, 286
Eam1104I CTCTTC 2 cut(s) 111, 139
EarI CTCTTC 2 cut(s) 111, 139
Eco130I CCWWGG 3 cut(s) 45, 247, 289
Eco47I GGWCC 1 cut(s) 320
EcoO109I RGGNCCY 1 cut(s) 320
EcoT14I CCWWGG 3 cut(s) 45, 247, 289
ErhI CCWWGG 3 cut(s) 45, 247, 289
FaeI CATG 3 cut(s) 182, 251, 293
FaiI YATR 7 cut(s) 32, 62, 64, 180, 249, 291, 357
FalI AAGNNNNNCTT 1 cut(s) 376
FatI CATG 3 cut(s) 178, 247, 289
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 2 cut(s) 259, 298
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 1 cut(s) 269
Fsp4HI GCNGC 1 cut(s) 42
FspBI CTAG 2 cut(s) 219, 273
GluI GCNGC 1 cut(s) 42
HaeIII GGCC 3 cut(s) 35, 185, 288
HapII CCGG 1 cut(s) 352
Hin1II CATG 3 cut(s) 182, 251, 293
HpaII CCGG 1 cut(s) 352
HphI GGTGA 3 cut(s) 103, 293, 293
Hpy166II GTNNAC 1 cut(s) 58
Hpy188III TCNNGA 3 cut(s) 80, 179, 311
Hpy8I GTNNAC 1 cut(s) 58
HpyAV CCTTC 2 cut(s) 79, 88
HpyCH4III ACNGT 2 cut(s) 340, 348
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 3 cut(s) 4, 11, 224
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 155
HpyF3I CTNAG 1 cut(s) 23
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 3 cut(s) 182, 251, 293
Ksp22I TGATCA 2 cut(s) 259, 298
Kzo9I GATC 2 cut(s) 259, 298
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 5 cut(s) 49, 149, 239, 279, 365
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 1 cut(s) 304
MaeI CTAG 2 cut(s) 219, 273
MaeII ACGT 1 cut(s) 54
MalI GATC 2 cut(s) 261, 300
MboI GATC 2 cut(s) 259, 298
MboII GAAGA 2 cut(s) 128, 156
MhlI GDGCHC 1 cut(s) 54
MlsI TGGCCA 2 cut(s) 35, 288
MluNI TGGCCA 2 cut(s) 35, 288
MnlI CCTC 6 cut(s) 114, 175, 254, 318, 333, 361
Mox20I TGGCCA 2 cut(s) 35, 288
MscI TGGCCA 2 cut(s) 35, 288
MslI CAYNNNNRTG 1 cut(s) 294
Msp20I TGGCCA 2 cut(s) 35, 288
MspI CCGG 1 cut(s) 352
MwoI GCNNNNNNNGC 2 cut(s) 41, 155
NcoI CCATGG 2 cut(s) 247, 289
NdeII GATC 2 cut(s) 259, 298
NlaIII CATG 3 cut(s) 182, 251, 293
NlaIV GGNNCC 1 cut(s) 321
PagI TCATGA 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 139
PkrI GCNGC 1 cut(s) 43
Ppu21I YACGTR 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 1 cut(s) 320
PspPPI RGGWCCY 1 cut(s) 320
RseI CAYNNNNRTG 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 139
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 2 cut(s) 259, 298
Sau96I GGNCC 1 cut(s) 320
SduI GDGCHC 1 cut(s) 54
SetI ASST 7 cut(s) 24, 57, 73, 79, 90, 310, 372
SfaNI GCATC 1 cut(s) 304
SinI GGWCC 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SsiI CCGC 1 cut(s) 317
SspMI CTAG 2 cut(s) 219, 273
StyI CCWWGG 3 cut(s) 45, 247, 289
TaaI ACNGT 2 cut(s) 340, 348
TaiI ACGT 1 cut(s) 57
TaqI TCGA 1 cut(s) 212
TscAI CASTG 1 cut(s) 43
TseI GCWGC 1 cut(s) 41
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 320
XcmI CCANNNNNNNNNTGG 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 57
XspI CTAG 2 cut(s) 219, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.