Rw7G020800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011487
Physical Location & Seq
Forward (+)
23815273 .. 23815969
697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020800.1

Sequence Viewer

Length: 312 bp
ATGCAGTCTGCAGAGGAGAAGATCAGTAACTTGGCCAGTGCTGCCAAGGAGAAAGTAGAGACTCACAAAGCCAAAACGGAAGAGAAGGTTGAGAAGATGACAGCAAGGACAGAGGAAGAGAAGAGAATAGCGGAGGAGAAGAGAAGAGCTAAAGAGGCAGAAGCGAAGATGAAGCTGCATGAGTCCAAAGCCAAGCATGAAGCCGATAAACTGACTACTGGTGCTCCTGCAACTGATGCCGGTGCTCCGATGGGTAGGACCGCAGTTCCATCGTATCCCCTGGGAGGTTACAGCACCCGAAAACTGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

11.31

Weight (kDa)

9.3

Isoelectric Point (pI)

58.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_1 PF03760 1 - 70 8.9e-22 Late embryogenesis abundant (LEA) group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 131, 261
AcoI YGGCCR 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 284
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 2 cut(s) 149, 175
AluI AGCT 2 cut(s) 149, 175
Alw21I GWGCWC 2 cut(s) 226, 247
Alw26I GTCTC 1 cut(s) 53
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 2 cut(s) 41, 175
AspS9I GGNCC 1 cut(s) 258
AvaII GGWCC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 35
Bbv12I GWGCWC 2 cut(s) 226, 247
BbvI GCAGC 2 cut(s) 28, 162
BccI CCATC 2 cut(s) 244, 277
BcgI CGANNNNNNTGC 2 cut(s) 252, 286
BciT130I CCWGG 1 cut(s) 281
BciVI GTATCC 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 1 cut(s) 310
BfmI CTRYAG 1 cut(s) 9
BfuI GTATCC 1 cut(s) 285
BisI GCNGC 2 cut(s) 42, 176
BlsI GCNGC 2 cut(s) 43, 177
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 1 cut(s) 226
BsaJI CCNNGG 3 cut(s) 45, 279, 280
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 2 cut(s) 36, 223
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 3 cut(s) 45, 279, 280
BseLI CCNNNNNNNGG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 36, 223
BseRI GAGGAG 2 cut(s) 29, 149
BseXI GCAGC 2 cut(s) 28, 162
BshFI GGCC 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 226, 247
BsiSI CCGG 1 cut(s) 240
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 53
BsnI GGCC 1 cut(s) 35
Bsp1286I GDGCHC 2 cut(s) 226, 247
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 2 cut(s) 131, 261
BspANI GGCC 1 cut(s) 35
BspMAI CTGCAG 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 2 cut(s) 36, 223
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 3 cut(s) 45, 279, 280
BssMI GATC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 281
Bst6I CTCTTC 5 cut(s) 75, 111, 116, 134, 139
BstAPI GCANNNNNTGC 1 cut(s) 236
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 3 cut(s) 41, 155, 236
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 28, 162
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 1 cut(s) 258
CviAII CATG 2 cut(s) 179, 197
CviJI RGCY 6 cut(s) 35, 71, 149, 175, 191, 203
CviKI_1 RGCY 6 cut(s) 35, 71, 149, 175, 191, 203
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 5 cut(s) 75, 111, 116, 134, 139
EarI CTCTTC 5 cut(s) 75, 111, 116, 134, 139
Eco130I CCWWGG 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 182, 200
FaiI YATR 2 cut(s) 180, 198
FatI CATG 2 cut(s) 178, 196
Fnu4HI GCNGC 2 cut(s) 42, 176
Fsp4HI GCNGC 2 cut(s) 42, 176
FspBI CTAG 1 cut(s) 310
GluI GCNGC 2 cut(s) 42, 176
HaeIII GGCC 1 cut(s) 35
HapII CCGG 1 cut(s) 240
Hin1II CATG 2 cut(s) 182, 200
HinfI GANTC 2 cut(s) 61, 182
HpaII CCGG 1 cut(s) 240
Hpy188I TCNGA 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 4, 11, 178, 230
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 155, 236
Hsp92II CATG 2 cut(s) 182, 200
Kzo9I GATC 1 cut(s) 21
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 2 cut(s) 229, 250
LpnPI CCDG 6 cut(s) 49, 204, 240, 253, 266, 293
Lsp1109I GCAGC 2 cut(s) 28, 162
LweI GCATC 1 cut(s) 226
MaeI CTAG 1 cut(s) 310
MaeIII GTNAC 2 cut(s) 26, 287
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 8 cut(s) 31, 92, 106, 128, 133, 151, 156, 178
MhlI GDGCHC 2 cut(s) 226, 247
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 2 cut(s) 55, 191
MnlI CCTC 5 cut(s) 7, 106, 127, 148, 278
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 35
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 41, 155, 236
NdeII GATC 1 cut(s) 21
NlaIII CATG 2 cut(s) 182, 200
PasI CCCWGGG 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 139
PkrI GCNGC 2 cut(s) 43, 177
PleI GAGTC 2 cut(s) 55, 190
PpsI GAGTC 2 cut(s) 55, 190
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 1 cut(s) 258
PstI CTGCAG 1 cut(s) 13
SapI GCTCTTC 1 cut(s) 139
SatI GCNGC 2 cut(s) 42, 176
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 1 cut(s) 258
SchI GAGTC 2 cut(s) 55, 191
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 2 cut(s) 226, 247
SetI ASST 4 cut(s) 90, 151, 177, 289
SfaNI GCATC 1 cut(s) 226
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 258
SsiI CCGC 2 cut(s) 131, 261
SspMI CTAG 1 cut(s) 310
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 45
TscAI CASTG 1 cut(s) 43
TseI GCWGC 2 cut(s) 41, 175
TspDTI ATGAA 2 cut(s) 185, 213
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 258
XspI CTAG 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.