AT2G35880

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006819
Physical Location & Seq
Reverse (-)
15062856 .. 15066056
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35880.2

Sequence Viewer

Length: 1299 bp
ATGGCGTCCGAGGATTTGAATATTGTTGCGGAATCAAAGAAAGGGGAAGAGAATGTTATTGTTGATAATTCCAAGGACATGAATAGACCTGAGAATCTTGACTTATCTACTGAGAAAACTGATACTGCCAATGAGAATGGTCCAAAAGATGAAGCTTCTAAGCTGGTAAAGGAAGCAGACCTCCCTGAGTCTGGTACTTCAGTGAAGAGTAAAACAGCTAAGGATAATAAACCTGTTAAGAGAAAGTCAGGGACGTTTTCTCGGAGCCCGAGATTCATGTCTCAGAGCTCTTCATTTCCAACTAAAGGAGCTTATACTGATATTACTAGAAAGAGCATTGATGCCACAACATCAAAGACTAGTCTTAAACCCGTCGTTGCTGGTGGATCCAAACCAAAAGCCACTCCATCTTCAAGCAGCGGTGTTTCCGCAAAGCGCACCAGTTTAGTATCTGCGCCTCTCAAAAAGCAAACTATGCCTGTGAAAACCATTTCTAAGGATGCAGCTTCAGGCCCTACTTCCAAATTAGGAGATGAAGGTTCTAAATCCATCAAAGAAGAAACTGCGGGGAAGGATGTCGAGGAGGCTGGATCAACCACCGCTGTGGTGGCAGATAAAGTATCTAACCCGTTGAAAGCTGAAATGGCAAGCAAGGACGACGAAGACACTCGTTCAACTACCACTTCAACTTCTACACCTCGTGGGCGGAGAAGCAGTGTTGGATCGGCATCTGGATTTTCGTTCAGGTTGGAGGAACGGGCTGAAAAACGGAAGGAGTTTTATATGAAACTAGAAGAGAAGATCCATGCAAAGGAAGTAGAGAAGACCAATTTGCAGGCGAAATCAAAGGAGAGCCAGGAAGAAGAGATCAAAAGGTTGAGGAAGAGCTTGACGTTCAAGGCTGGTCCCATGCCCAGTTTCTACAAAGAACCCCCACCTAAGGTTGAATTGAAGAAGATACCAACTACTCGTCCTAAGTCTCCAAAGCTAGGACGCAGAAAGAGTAGCAGTGATGCAACAGGAGGTGAAGCCGCTCCTCGCGTGACAAAACCAAAAGACTCATCATCATCTACAGTGAAGAAACCAATAACAAAGTCTCAGCCAAAGCTTGAAACTCAAGAGAAATCAGTGAAGGCCAAAGAAAAGAAAAAGAAAGTGAAGAAAGAAGAAGCAGAGAAGAGAGGAGAAGAAGAGAAGGCTACTGCCGTGGCTGCGAAACCTGAAGAGCAGAAACCAAACTCCAATAACATCCAAGTGAAGGCTGAGATAATGGCGAGTGAGGTTGTAGTGGGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

432

Amino Acids

46.7

Weight (kDa)

9.7

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 246 - 320 1.8e-25 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1034
AccII CGCG 1 cut(s) 1041
AciI CCGC 7 cut(s) 29, 420, 429, 566, 600, 706, 1032
AclWI GGATC 5 cut(s) 381, 394, 598, 730, 796
AcuI CTGAAG 3 cut(s) 183, 492, 1242
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 701
AfaI GTAC 1 cut(s) 196
AfiI CCNNNNNNNGG 6 cut(s) 191, 305, 811, 940, 989, 1258
AgsI TTSAA 9 cut(s) 19, 414, 634, 675, 687, 898, 947, 952, 1112
AhlI ACTAGT 1 cut(s) 359
AjnI CCWGG 1 cut(s) 855
AleI CACNNNNGTG 1 cut(s) 602
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 3 cut(s) 285, 984, 1101
AlwI GGATC 5 cut(s) 381, 394, 598, 730, 796
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 2 cut(s) 511, 1134
ApeKI GCWGC 3 cut(s) 417, 503, 1211
AspLEI GCGC 2 cut(s) 438, 457
AspS9I GGNCC 3 cut(s) 140, 512, 905
AsuHPI GGTGA 1 cut(s) 1037
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 2 cut(s) 140, 905
AxyI CCTNAGG 1 cut(s) 939
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
BamHI GGATCC 1 cut(s) 386
BanII GRGCYC 2 cut(s) 269, 290
BauI CACGAG 1 cut(s) 699
BbsI GAAGAC 2 cut(s) 669, 830
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 429, 515, 1198
BccI CCATC 2 cut(s) 415, 557
BceAI ACGGC 1 cut(s) 1190
BciT130I CCWGG 1 cut(s) 857
BcoDI GTCTC 3 cut(s) 285, 984, 1101
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 4 cut(s) 327, 360, 791, 989
BfmI CTRYAG 1 cut(s) 1071
BisI GCNGC 4 cut(s) 418, 504, 1032, 1212
BlsI GCNGC 4 cut(s) 419, 505, 1033, 1213
Bme1390I CCNGG 1 cut(s) 857
Bme18I GGWCC 2 cut(s) 140, 905
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 3 cut(s) 140, 512, 905
BmiI GGNNCC 3 cut(s) 266, 388, 907
BmrFI CCNGG 1 cut(s) 857
BmrI ACTGGG 1 cut(s) 909
BmsI GCATC 4 cut(s) 331, 490, 737, 1003
BmuI ACTGGG 1 cut(s) 909
BpiI GAAGAC 2 cut(s) 669, 830
Bpu10I CCTNAGC 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 1101
BsaBI GATNNNNATC 1 cut(s) 727
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 9, 72, 1206
BsaXI ACNNNNNCTCC 2 cut(s) 700, 730
Bsc4I CCNNNNNNNGG 6 cut(s) 191, 305, 811, 940, 989, 1258
Bse1I ACTGG 2 cut(s) 441, 915
Bse21I CCTNAGG 1 cut(s) 939
Bse8I GATNNNNATC 1 cut(s) 727
BseBI CCWGG 1 cut(s) 857
BseDI CCNNGG 3 cut(s) 9, 72, 1206
BseGI GGATG 3 cut(s) 505, 580, 1248
BseJI GATNNNNATC 1 cut(s) 727
BseLI CCNNNNNNNGG 6 cut(s) 191, 305, 811, 940, 989, 1258
BseMII CTCAG 6 cut(s) 81, 102, 177, 296, 1112, 1254
BseNI ACTGG 2 cut(s) 441, 915
BseRI GAGGAG 3 cut(s) 596, 1026, 1197
BseXI GCAGC 3 cut(s) 429, 515, 1198
Bsh1236I CGCG 1 cut(s) 1041
BshFI GGCC 2 cut(s) 513, 1136
BsiHKAI GWGCWC 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 268
BslFI GGGAC 2 cut(s) 265, 891
BslI CCNNNNNNNGG 6 cut(s) 191, 305, 811, 940, 989, 1258
BsmAI GTCTC 3 cut(s) 285, 984, 1101
BsmFI GGGAC 2 cut(s) 265, 891
BsnI GGCC 2 cut(s) 513, 1136
BsoBI CYCGRG 1 cut(s) 268
Bsp1286I GDGCHC 2 cut(s) 269, 290
Bsp143I GATC 5 cut(s) 386, 590, 722, 801, 867
BspACI CCGC 7 cut(s) 29, 420, 429, 566, 600, 706, 1032
BspANI GGCC 2 cut(s) 513, 1136
BspCNI CTCAG 6 cut(s) 82, 103, 178, 295, 1111, 1255
BspFNI CGCG 1 cut(s) 1041
BspLI GGNNCC 3 cut(s) 266, 388, 907
BspPI GGATC 5 cut(s) 381, 394, 598, 730, 796
BspQI GCTCTTC 3 cut(s) 295, 878, 1218
BsrBI CCGCTC 1 cut(s) 1034
BsrI ACTGG 2 cut(s) 441, 915
BssECI CCNNGG 3 cut(s) 9, 72, 1206
BssMI GATC 5 cut(s) 386, 590, 722, 801, 867
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 699
BssT1I CCWWGG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 699
Bst2UI CCWGG 1 cut(s) 857
Bst4CI ACNGT 1 cut(s) 1075
Bst6I CTCTTC 9 cut(s) 42, 200, 295, 789, 858, 878, 1172, 1185, 1218
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 475
BstC8I GCNNGC 2 cut(s) 649, 837
BstDSI CCRYGG 1 cut(s) 1206
BstF5I GGATG 3 cut(s) 505, 580, 1248
BstFNI CGCG 1 cut(s) 1041
BstHHI GCGC 2 cut(s) 438, 457
BstKTI GATC 5 cut(s) 389, 593, 725, 804, 870
BstMAI GTCTC 3 cut(s) 285, 984, 1101
BstMBI GATC 5 cut(s) 386, 590, 722, 801, 867
BstMWI GCNNNNNNNGC 4 cut(s) 475, 608, 644, 1211
BstNI CCWGG 1 cut(s) 857
BstSCI CCNGG 1 cut(s) 855
BstSFI CTRYAG 1 cut(s) 1071
BstUI CGCG 1 cut(s) 1041
BstV1I GCAGC 3 cut(s) 429, 515, 1198
BstV2I GAAGAC 2 cut(s) 669, 830
BstX2I RGATCY 2 cut(s) 386, 801
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 2 cut(s) 386, 801
Bsu36I CCTNAGG 1 cut(s) 939
BsuRI GGCC 2 cut(s) 513, 1136
BtgI CCRYGG 1 cut(s) 1206
BtsCI GGATG 3 cut(s) 505, 580, 1248
BtsI GCAGTG 2 cut(s) 721, 1015
BtsIMutI CAGTG 5 cut(s) 207, 721, 1015, 1080, 1134
Cac8I GCNNGC 2 cut(s) 649, 837
CfoI GCGC 2 cut(s) 438, 457
Cfr13I GGNCC 3 cut(s) 140, 512, 905
CseI GACGC 1 cut(s) 1002
Csp6I GTAC 1 cut(s) 195
CviAII CATG 4 cut(s) 79, 277, 806, 910
CviQI GTAC 1 cut(s) 195
DpnI GATC 5 cut(s) 388, 592, 724, 803, 869
DpnII GATC 5 cut(s) 386, 590, 722, 801, 867
DraIII CACNNNGTG 1 cut(s) 701
Eam1104I CTCTTC 9 cut(s) 42, 200, 295, 789, 858, 878, 1172, 1185, 1218
EarI CTCTTC 9 cut(s) 42, 200, 295, 789, 858, 878, 1172, 1185, 1218
EciI GGCGGA 1 cut(s) 721
Ecl136II GAGCTC 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 2 cut(s) 269, 290
Eco47I GGWCC 2 cut(s) 140, 905
Eco53kI GAGCTC 1 cut(s) 288
Eco57I CTGAAG 3 cut(s) 183, 492, 1242
Eco81I CCTNAGG 1 cut(s) 939
Eco88I CYCGRG 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 288
EcoO109I RGGNCCY 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 855
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 2 cut(s) 269, 290
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 4 cut(s) 82, 280, 809, 913
FaiI YATR 8 cut(s) 80, 278, 315, 476, 783, 785, 807, 911
FaqI GGGAC 2 cut(s) 265, 891
FatI CATG 4 cut(s) 78, 276, 805, 909
FauI CCCGC 1 cut(s) 559
Fnu4HI GCNGC 4 cut(s) 418, 504, 1032, 1212
FokI GGATG 3 cut(s) 512, 587, 1235
FriOI GRGCYC 2 cut(s) 269, 290
Fsp4HI GCNGC 4 cut(s) 418, 504, 1032, 1212
FspBI CTAG 4 cut(s) 327, 360, 791, 989
GlaI GCGC 2 cut(s) 437, 456
GluI GCNGC 4 cut(s) 418, 504, 1032, 1212
HaeIII GGCC 2 cut(s) 513, 1136
HgaI GACGC 1 cut(s) 1002
HhaI GCGC 2 cut(s) 438, 457
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 82, 280, 809, 913
Hin6I GCGC 2 cut(s) 436, 455
HinP1I GCGC 2 cut(s) 436, 455
HindIII AAGCTT 2 cut(s) 153, 1106
HinfI GANTC 5 cut(s) 32, 94, 188, 273, 1058
HphI GGTGA 1 cut(s) 1037
Hpy188I TCNGA 3 cut(s) 10, 264, 285
Hpy188III TCNNGA 3 cut(s) 98, 732, 1118
Hpy99I CGWCG 2 cut(s) 377, 662
HpyAV CCTTC 6 cut(s) 530, 565, 766, 1126, 1189, 1252
HpyCH4III ACNGT 1 cut(s) 1075
HpyCH4IV ACGT 2 cut(s) 254, 893
HpyCH4V TGCA 4 cut(s) 503, 809, 835, 1016
HpyF10VI GCNNNNNNNGC 4 cut(s) 475, 608, 644, 1211
HpySE526I ACGT 2 cut(s) 254, 893
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 82, 280, 809, 913
HspAI GCGC 2 cut(s) 436, 455
Kzo9I GATC 5 cut(s) 386, 590, 722, 801, 867
LguI GCTCTTC 3 cut(s) 295, 878, 1218
LmnI GCTCC 3 cut(s) 264, 308, 1039
Lsp1109I GCAGC 3 cut(s) 429, 515, 1198
LweI GCATC 4 cut(s) 331, 490, 737, 1003
MaeI CTAG 4 cut(s) 327, 360, 791, 989
MaeII ACGT 2 cut(s) 254, 893
MaeIII GTNAC 1 cut(s) 1042
MalI GATC 5 cut(s) 388, 592, 724, 803, 869
MbiI CCGCTC 1 cut(s) 1034
MboI GATC 5 cut(s) 386, 590, 722, 801, 867
MflI RGATCY 2 cut(s) 386, 801
MhlI GDGCHC 2 cut(s) 269, 290
MluCI AATT 4 cut(s) 67, 524, 829, 947
MlyI GAGTC 2 cut(s) 197, 1052
MmeI TCCRAC 3 cut(s) 323, 700, 729
MseI TTAA 2 cut(s) 237, 366
MslI CAYNNNNRTG 2 cut(s) 602, 1253
MspA1I CMGCKG 2 cut(s) 420, 602
MspR9I CCNGG 1 cut(s) 857
MvaI CCWGG 1 cut(s) 857
MvnI CGCG 1 cut(s) 1041
MwoI GCNNNNNNNGC 4 cut(s) 475, 608, 644, 1211
NdeII GATC 5 cut(s) 386, 590, 722, 801, 867
NlaIII CATG 4 cut(s) 82, 280, 809, 913
NlaIV GGNNCC 3 cut(s) 266, 388, 907
NmuCI GTSAC 1 cut(s) 1042
OliI CACNNNNGTG 1 cut(s) 602
PciSI GCTCTTC 3 cut(s) 295, 878, 1218
PfeI GAWTC 3 cut(s) 32, 94, 273
PkrI GCNGC 4 cut(s) 419, 505, 1033, 1213
PleI GAGTC 2 cut(s) 196, 1052
PpsI GAGTC 2 cut(s) 196, 1052
Psp124BI GAGCTC 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 855
PspGI CCWGG 1 cut(s) 855
PspN4I GGNNCC 3 cut(s) 266, 388, 907
PspPI GGNCC 3 cut(s) 140, 512, 905
PsuI RGATCY 2 cut(s) 386, 801
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
RseI CAYNNNNRTG 2 cut(s) 602, 1253
SacI GAGCTC 1 cut(s) 290
SapI GCTCTTC 3 cut(s) 295, 878, 1218
SaqAI TTAA 2 cut(s) 237, 366
SatI GCNGC 4 cut(s) 418, 504, 1032, 1212
Sau3AI GATC 5 cut(s) 386, 590, 722, 801, 867
Sau96I GGNCC 3 cut(s) 140, 512, 905
SchI GAGTC 2 cut(s) 197, 1052
ScrFI CCNGG 1 cut(s) 857
SduI GDGCHC 2 cut(s) 269, 290
SfaNI GCATC 4 cut(s) 331, 490, 737, 1003
SfcI CTRYAG 1 cut(s) 1071
SinI GGWCC 2 cut(s) 140, 905
SmiMI CAYNNNNRTG 2 cut(s) 602, 1253
SmlI CTYRAG 1 cut(s) 1116
SmoI CTYRAG 1 cut(s) 1116
SpeI ACTAGT 1 cut(s) 359
Sse9I AATT 4 cut(s) 67, 524, 829, 947
SsiI CCGC 7 cut(s) 29, 420, 429, 566, 600, 706, 1032
SspI AATATT 1 cut(s) 22
SspMI CTAG 4 cut(s) 327, 360, 791, 989
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 855
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 1075
TaiI ACGT 2 cut(s) 257, 896
TaqI TCGA 1 cut(s) 579
TasI AATT 4 cut(s) 67, 524, 829, 947
TauI GCSGC 1 cut(s) 1034
TfiI GAWTC 3 cut(s) 32, 94, 273
Tru1I TTAA 2 cut(s) 237, 366
Tru9I TTAA 2 cut(s) 237, 366
TscAI CASTG 5 cut(s) 207, 721, 1015, 1080, 1134
TseFI GTSAC 1 cut(s) 1042
TseI GCWGC 3 cut(s) 417, 503, 1211
Tsp45I GTSAC 1 cut(s) 1042
TspDTI ATGAA 6 cut(s) 95, 165, 265, 282, 549, 800
TspGWI ACGGA 1 cut(s) 784
TspRI CASTG 5 cut(s) 207, 721, 1015, 1080, 1134
VpaK11BI GGWCC 2 cut(s) 140, 905
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XspI CTAG 4 cut(s) 327, 360, 791, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.