Rroxscaffold_6G00404340

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006819
Physical Location & Seq
Reverse (-)
26877306 .. 26881294
3989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404340.1

Sequence Viewer

Length: 1506 bp
ATGGAGTCTGAAAATGGGATTGCTTTAGGGGATGAGAATTGTGCTGTTGTGGAAAAACATGTAGAAGAATCAGTACCGGATTTGAACATTGAAGGAAAGAATGTTGATGCTAATATAGAAGTTCCTACCATGAATGGAGTATCTGAACCTGTAAGCAAAGATGAAGGCATTAACCCTTCAGGAGGTGCAGTTGAAGTTGCTGCAAGTGTTCCGCTTGGTAAAAATTCAAAAACAAAAAAGGATCCTCGTGCTCCAAACAATGGTGTTTCAAAGGGAAAGTTGGCCAAGGATAAACCCAGTGTGAAAGGTGCAACTCCAGTTTCTCGTACACAAAGGGCAATACTTTCTCAGAGTCTTTCGTTCCCAGCCAGGGGAGCTCGTGTTGATGCTATGAAGAAGAGCATTGATGTTTATCCAGTGAAAACAGAAGTCAAACACACTCGAGGGAATGCAACCAAAGCTGAGGCCCCTTTTCCATCTTCTCGTTTGAATCATCAAAATAGGCGTGCATCCACAGGAATACACTCAAATGATGAGAACAAAGCTGGTGGAGCTTCAGTTAGGAGGACTACTTTAGCATCAATACCTAGCATGAGATCCTCTGCATCAATGAAGTCTGGCTCTGTGAGTAAACCTGCTAATAGTTCATATGATGTGACCCGATCAGTTGGGCAGATCTTAAGTGCTGTAAAAACAACCCTGCCAATCAAAGAGGATGATGATGCTCATTCCATTGCTTCAAGCACTACACCTGGTGGGCGGAGGAGCAGTGGTTCTGCATTTTCCTTCAGGTTGGATGAACGTGCTGAAAAAAGGAAGGAGTTCTTTACAAAGCTAGAAGAGAAGATACAGGCTAAGGAAGCAGAAAAAAATAATTCGCAAGCAAAATCAAAGGAAAACCAGGAAGCAGAGATCAAGCAACTGAGGAAGAGCCTGACATTTAGGGCAGCTCCCATGCCAACTTTCTATAAAGAGCCTCCTCCAAAAATTGAAATAAAGAAGATACCGACCACACGTCCCATATCTCCAAAGCTTGGAAGGCACAAGAACTCCATTTCTTCATTGAACAACCCCTCAGAAGGTGGTGGGACGTGTCTTAGCCCGCGTCTGAATCAAGAACAGAACAATTCAAACAAAGGGTTAAAGGCAACAGGTGAAAAAGATGTCACAGAATCAAAGAAGCCTATCAGGAAGTCCCAACATCGGCTTCATTCTCAGGAAAATGTGGCTGCTAAAGCTGAAGGAAAGCCCTGTAAGTCACAACTGAAAGTGGCCAGCAAAGAAGAAAAAGAAAGCCCGAAGGCATGCACTGGAGAGACAGAAGAAAGCTTCCAAGACCAATCTGAACATCTTTCTGAATGCAAACATAAGATAGAGCCTGAAGTGAGTGTTGACCAGACCAAAGAACCAGTTGTCAGTGCAGCTCCTGCTCCTGAGATCATGCCTCAGGAAGTTACCATTGCAGCTCCTGAGATCATGCCTCGAGGAGTTGCCATTGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

501

Amino Acids

53.94

Weight (kDa)

9.4

Isoelectric Point (pI)

57.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 261 - 329 4.5e-25 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1482
Acc36I ACCTGC 1 cut(s) 643
AccB7I CCANNNNNTGG 2 cut(s) 260, 1034
AccII CGCG 1 cut(s) 1105
AciI CCGC 3 cut(s) 212, 760, 1103
AclWI GGATC 3 cut(s) 236, 249, 591
AcoI YGGCCR 2 cut(s) 282, 1272
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 5 cut(s) 162, 540, 772, 1260, 1401
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 2 cut(s) 75, 328
AfiI CCNNNNNNNGG 7 cut(s) 182, 260, 370, 371, 1034, 1079, 1203
AflII CTTAAG 1 cut(s) 679
AflIII ACRYGT 3 cut(s) 58, 1013, 1091
AhdI GACNNNNNGTC 1 cut(s) 1014
AjiI CACGTC 2 cut(s) 1016, 1092
AjnI CCWGG 3 cut(s) 368, 751, 900
AjuI GAANNNNNNNTTGG 2 cut(s) 1191, 1223
AloI GAACNNNNNNTCC 2 cut(s) 757, 789
Alw21I GWGCWC 2 cut(s) 253, 379
Alw26I GTCTC 1 cut(s) 1310
AlwI GGATC 3 cut(s) 236, 249, 591
AlwNI CAGNNNCTG 2 cut(s) 1427, 1469
Ama87I CYCGRG 2 cut(s) 441, 1482
AoxI GGCC 3 cut(s) 282, 465, 1272
ApeKI GCWGC 5 cut(s) 200, 947, 1229, 1421, 1463
ApoI RAATTY 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 821
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 1166
AvaI CYCGRG 2 cut(s) 441, 1482
AxyI CCTNAGG 1 cut(s) 1446
BalI TGGCCA 2 cut(s) 284, 1274
BamHI GGATCC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 379
BarI GAAGNNNNNNTAC 4 cut(s) 57, 89, 831, 863
BauI CACGAG 2 cut(s) 246, 378
Bbv12I GWGCWC 2 cut(s) 253, 379
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 5 cut(s) 187, 959, 1216, 1433, 1475
BccI CCATC 1 cut(s) 484
BciT130I CCWGG 3 cut(s) 370, 753, 902
BcoDI GTCTC 1 cut(s) 1310
BfaI CTAG 2 cut(s) 588, 836
BfrI CTTAAG 1 cut(s) 679
BfuAI ACCTGC 1 cut(s) 643
BglII AGATCT 1 cut(s) 675
BisI GCNGC 5 cut(s) 201, 948, 1230, 1422, 1464
BlsI GCNGC 5 cut(s) 202, 949, 1231, 1423, 1465
Bme1390I CCNGG 3 cut(s) 370, 753, 902
BmeRI GACNNNNNGTC 1 cut(s) 1014
BmeT110I CYCGRG 2 cut(s) 441, 1482
BmgBI CACGTC 2 cut(s) 1016, 1092
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 2 cut(s) 243, 468
BmrFI CCNGG 3 cut(s) 370, 753, 902
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
BmuI ACTGGG 1 cut(s) 291
BpmI CTGGAG 2 cut(s) 300, 1332
Bpu10I CCTNAGC 2 cut(s) 462, 855
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 2 cut(s) 285, 369
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 7 cut(s) 182, 260, 370, 371, 1034, 1079, 1203
Bse1I ACTGG 5 cut(s) 297, 317, 416, 1315, 1409
Bse21I CCTNAGG 1 cut(s) 1446
Bse3DI GCAATG 2 cut(s) 732, 1458
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 3 cut(s) 370, 753, 902
BseDI CCNNGG 2 cut(s) 285, 369
BseGI GGATG 4 cut(s) 37, 509, 721, 802
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 7 cut(s) 182, 260, 370, 371, 1034, 1079, 1203
BseMI GCAATG 2 cut(s) 732, 1458
BseMII CTCAG 8 cut(s) 362, 453, 914, 1089, 1229, 1425, 1460, 1461
BseNI ACTGG 5 cut(s) 297, 317, 416, 1315, 1409
BseRI GAGGAG 3 cut(s) 778, 969, 1500
BseXI GCAGC 5 cut(s) 187, 959, 1216, 1433, 1475
BseYI CCCAGC 1 cut(s) 364
BsgI GTGCAG 2 cut(s) 207, 1440
Bsh1236I CGCG 1 cut(s) 1105
BshFI GGCC 3 cut(s) 284, 467, 1274
BsiHKAI GWGCWC 2 cut(s) 253, 379
BsiHKCI CYCGRG 2 cut(s) 441, 1482
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 3 cut(s) 1002, 1102, 1180
BslI CCNNNNNNNGG 7 cut(s) 182, 260, 370, 371, 1034, 1079, 1203
BsmAI GTCTC 1 cut(s) 1310
BsmFI GGGAC 3 cut(s) 1002, 1102, 1180
BsmI GAATGC 2 cut(s) 454, 1364
BsnI GGCC 3 cut(s) 284, 467, 1274
BsoBI CYCGRG 2 cut(s) 441, 1482
Bsp1286I GDGCHC 2 cut(s) 253, 379
Bsp143I GATC 7 cut(s) 241, 596, 662, 675, 912, 1437, 1473
BspACI CCGC 3 cut(s) 212, 760, 1103
BspANI GGCC 3 cut(s) 284, 467, 1274
BspCNI CTCAG 8 cut(s) 361, 454, 915, 1088, 1228, 1426, 1459, 1462
BspFNI CGCG 1 cut(s) 1105
BspLI GGNNCC 2 cut(s) 243, 468
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 3 cut(s) 236, 249, 591
BspQI GCTCTTC 2 cut(s) 392, 923
BspTI CTTAAG 1 cut(s) 679
BsrDI GCAATG 2 cut(s) 732, 1458
BsrI ACTGG 5 cut(s) 297, 317, 416, 1315, 1409
BssECI CCNNGG 2 cut(s) 285, 369
BssMI GATC 7 cut(s) 241, 596, 662, 675, 912, 1437, 1473
BssSI CACGAG 2 cut(s) 246, 378
BssT1I CCWWGG 1 cut(s) 285
Bst2BI CACGAG 2 cut(s) 246, 378
Bst2UI CCWGG 3 cut(s) 370, 753, 902
Bst6I CTCTTC 3 cut(s) 392, 834, 923
BstAFI CTTAAG 1 cut(s) 679
BstAPI GCANNNNNTGC 1 cut(s) 1427
BstC8I GCNNGC 5 cut(s) 507, 882, 1103, 1276, 1306
BstENI CCTNNNNNAGG 1 cut(s) 180
BstF5I GGATG 4 cut(s) 37, 509, 721, 802
BstFNI CGCG 1 cut(s) 1105
BstKTI GATC 7 cut(s) 244, 599, 665, 678, 915, 1440, 1476
BstMAI GTCTC 1 cut(s) 1310
BstMBI GATC 7 cut(s) 241, 596, 662, 675, 912, 1437, 1473
BstMWI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 860, 1039, 1235, 1427
BstNI CCWGG 3 cut(s) 370, 753, 902
BstNSI RCATGY 2 cut(s) 62, 1308
BstSCI CCNGG 3 cut(s) 368, 751, 900
BstUI CGCG 1 cut(s) 1105
BstV1I GCAGC 5 cut(s) 187, 959, 1216, 1433, 1475
BstX2I RGATCY 3 cut(s) 241, 596, 675
BstYI RGATCY 3 cut(s) 241, 596, 675
Bsu36I CCTNAGG 1 cut(s) 1446
BsuRI GGCC 3 cut(s) 284, 467, 1274
BtrI CACGTC 2 cut(s) 1016, 1092
BtsCI GGATG 4 cut(s) 37, 509, 721, 802
BtsI GCAGTG 1 cut(s) 775
BtsIMutI CAGTG 5 cut(s) 304, 423, 775, 1308, 1423
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 5 cut(s) 507, 882, 1103, 1276, 1306
CaiI CAGNNNCTG 2 cut(s) 1427, 1469
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 1 cut(s) 1094
CsiI ACCWGGT 1 cut(s) 751
Csp6I GTAC 2 cut(s) 74, 327
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviAII CATG 7 cut(s) 59, 130, 592, 955, 1305, 1441, 1477
CviQI GTAC 2 cut(s) 74, 327
DpnI GATC 7 cut(s) 243, 598, 664, 677, 914, 1439, 1475
DpnII GATC 7 cut(s) 241, 596, 662, 675, 912, 1437, 1473
DraIII CACNNNGTG 1 cut(s) 755
DriI GACNNNNNGTC 1 cut(s) 1014
EaeI YGGCCR 2 cut(s) 282, 1272
Eam1104I CTCTTC 3 cut(s) 392, 834, 923
Eam1105I GACNNNNNGTC 1 cut(s) 1014
EarI CTCTTC 3 cut(s) 392, 834, 923
EciI GGCGGA 1 cut(s) 775
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 377
Eco57I CTGAAG 5 cut(s) 162, 540, 772, 1260, 1401
Eco81I CCTNAGG 1 cut(s) 1446
Eco88I CYCGRG 2 cut(s) 441, 1482
EcoICRI GAGCTC 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 3 cut(s) 368, 751, 900
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 7 cut(s) 62, 133, 595, 958, 1308, 1444, 1480
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 3 cut(s) 1002, 1102, 1180
FatI CATG 7 cut(s) 58, 129, 591, 954, 1304, 1440, 1476
FauI CCCGC 1 cut(s) 1110
FauNDI CATATG 1 cut(s) 649
Fnu4HI GCNGC 5 cut(s) 201, 948, 1230, 1422, 1464
FokI GGATG 4 cut(s) 44, 496, 728, 809
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 5 cut(s) 201, 948, 1230, 1422, 1464
FspBI CTAG 2 cut(s) 588, 836
GluI GCNGC 5 cut(s) 201, 948, 1230, 1422, 1464
GsaI CCCAGC 1 cut(s) 368
GsuI CTGGAG 2 cut(s) 300, 1332
HaeIII GGCC 3 cut(s) 284, 467, 1274
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 1094
Hin1II CATG 7 cut(s) 62, 133, 595, 958, 1308, 1444, 1480
HincII GTYRAC 1 cut(s) 1393
HindII GTYRAC 1 cut(s) 1393
HindIII AAGCTT 2 cut(s) 1031, 1327
HinfI GANTC 6 cut(s) 5, 68, 352, 490, 1111, 1172
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 1166
Hpy166II GTNNAC 3 cut(s) 329, 632, 1393
Hpy188I TCNGA 7 cut(s) 10, 145, 351, 1078, 1110, 1345, 1357
Hpy188III TCNNGA 7 cut(s) 180, 1115, 1189, 1217, 1433, 1448, 1469
Hpy8I GTNNAC 3 cut(s) 329, 632, 1393
HpyAV CCTTC 9 cut(s) 86, 158, 186, 796, 811, 1032, 1073, 1235, 1294
HpyCH4IV ACGT 3 cut(s) 802, 1015, 1091
HpyF10VI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 860, 1039, 1235, 1427
HpySE526I ACGT 3 cut(s) 802, 1015, 1091
Hsp92II CATG 7 cut(s) 62, 133, 595, 958, 1308, 1444, 1480
Kzo9I GATC 7 cut(s) 241, 596, 662, 675, 912, 1437, 1473
LguI GCTCTTC 2 cut(s) 392, 923
LmnI GCTCC 8 cut(s) 256, 374, 551, 765, 955, 1429, 1435, 1471
Lsp1109I GCAGC 5 cut(s) 187, 959, 1216, 1433, 1475
LweI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
MabI ACCWGGT 1 cut(s) 751
MaeI CTAG 2 cut(s) 588, 836
MaeII ACGT 3 cut(s) 802, 1015, 1091
MaeIII GTNAC 4 cut(s) 655, 1165, 1257, 1453
MalI GATC 7 cut(s) 243, 598, 664, 677, 914, 1439, 1475
MboI GATC 7 cut(s) 241, 596, 662, 675, 912, 1437, 1473
MflI RGATCY 3 cut(s) 241, 596, 675
MhlI GDGCHC 2 cut(s) 253, 379
MlsI TGGCCA 2 cut(s) 284, 1274
MluCI AATT 5 cut(s) 37, 223, 874, 987, 1126
MluNI TGGCCA 2 cut(s) 284, 1274
MlyI GAGTC 2 cut(s) 14, 361
MmeI TCCRAC 1 cut(s) 774
Mox20I TGGCCA 2 cut(s) 284, 1274
MroXI GAANNNNTTC 1 cut(s) 821
MscI TGGCCA 2 cut(s) 284, 1274
MseI TTAA 4 cut(s) 171, 680, 1142, 1504
MslI CAYNNNNRTG 1 cut(s) 528
Msp20I TGGCCA 2 cut(s) 284, 1274
MspCI CTTAAG 1 cut(s) 679
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 3 cut(s) 370, 753, 902
Mva1269I GAATGC 2 cut(s) 454, 1364
MvaI CCWGG 3 cut(s) 370, 753, 902
MvnI CGCG 1 cut(s) 1105
MwoI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 860, 1039, 1235, 1427
NdeI CATATG 1 cut(s) 649
NdeII GATC 7 cut(s) 241, 596, 662, 675, 912, 1437, 1473
NlaIII CATG 7 cut(s) 62, 133, 595, 958, 1308, 1444, 1480
NlaIV GGNNCC 2 cut(s) 243, 468
NmuCI GTSAC 3 cut(s) 655, 1165, 1257
NspI RCATGY 2 cut(s) 62, 1308
PaeI GCATGC 1 cut(s) 1308
PaeR7I CTCGAG 2 cut(s) 441, 1482
PciI ACATGT 1 cut(s) 58
PciSI GCTCTTC 2 cut(s) 392, 923
PctI GAATGC 2 cut(s) 454, 1364
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 4 cut(s) 68, 490, 1111, 1172
PflMI CCANNNNNTGG 2 cut(s) 260, 1034
PkrI GCNGC 5 cut(s) 202, 949, 1231, 1423, 1465
PleI GAGTC 2 cut(s) 13, 360
PpsI GAGTC 2 cut(s) 13, 360
PscI ACATGT 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 368, 751, 900
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 3 cut(s) 368, 751, 900
PspN4I GGNNCC 2 cut(s) 243, 468
PspPI GGNCC 1 cut(s) 466
PspXI VCTCGAGB 2 cut(s) 441, 1482
PstNI CAGNNNCTG 2 cut(s) 1427, 1469
PsuI RGATCY 3 cut(s) 241, 596, 675
RsaI GTAC 2 cut(s) 75, 328
RsaNI GTAC 2 cut(s) 74, 327
RseI CAYNNNNRTG 1 cut(s) 528
SacI GAGCTC 1 cut(s) 379
SapI GCTCTTC 2 cut(s) 392, 923
SaqAI TTAA 4 cut(s) 171, 680, 1142, 1504
SatI GCNGC 5 cut(s) 201, 948, 1230, 1422, 1464
Sau3AI GATC 7 cut(s) 241, 596, 662, 675, 912, 1437, 1473
Sau96I GGNCC 1 cut(s) 466
SchI GAGTC 2 cut(s) 14, 361
ScrFI CCNGG 3 cut(s) 370, 753, 902
SduI GDGCHC 2 cut(s) 253, 379
SexAI ACCWGGT 1 cut(s) 751
SfaNI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
Sfr274I CTCGAG 2 cut(s) 441, 1482
SlaI CTCGAG 2 cut(s) 441, 1482
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 3 cut(s) 441, 679, 1482
SmoI CTYRAG 3 cut(s) 441, 679, 1482
SphI GCATGC 1 cut(s) 1308
Sse9I AATT 5 cut(s) 37, 223, 874, 987, 1126
SsiI CCGC 3 cut(s) 212, 760, 1103
SspMI CTAG 2 cut(s) 588, 836
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 3 cut(s) 368, 751, 900
StyI CCWWGG 1 cut(s) 285
TaiI ACGT 3 cut(s) 805, 1018, 1094
TaqI TCGA 2 cut(s) 442, 1483
TasI AATT 5 cut(s) 37, 223, 874, 987, 1126
TfiI GAWTC 4 cut(s) 68, 490, 1111, 1172
Tru1I TTAA 4 cut(s) 171, 680, 1142, 1504
Tru9I TTAA 4 cut(s) 171, 680, 1142, 1504
TscAI CASTG 5 cut(s) 304, 423, 775, 1315, 1423
TseFI GTSAC 3 cut(s) 655, 1165, 1257
TseI GCWGC 5 cut(s) 200, 947, 1229, 1421, 1463
Tsp45I GTSAC 3 cut(s) 655, 1165, 1257
TspDTI ATGAA 8 cut(s) 146, 177, 407, 626, 636, 813, 1050, 1199
TspRI CASTG 5 cut(s) 304, 423, 775, 1315, 1423
Van91I CCANNNNNTGG 2 cut(s) 260, 1034
Vha464I CTTAAG 1 cut(s) 679
XagI CCTNNNNNAGG 1 cut(s) 180
XapI RAATTY 1 cut(s) 223
XceI RCATGY 2 cut(s) 62, 1308
XhoI CTCGAG 2 cut(s) 441, 1482
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 2 cut(s) 588, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.