MD12G1044500.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006819
Physical Location & Seq
Forward (+)
4970142 .. 4974732
4591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1044500.v1.1.491

Sequence Viewer

Length: 1476 bp
ATGAATATTTCGAGGTATTTCACAATGGAGTCTGAAAATGGGGTTACATTCGACGATGAGAGTTGTGTTATCGAGGAAAAACATGTAGATGTATCAGTACCGAGTTTGAGCAAAGAGGGAAAGAATGCTGATGGTAATGCTGAAGTTCCTGCCGTGAATGGAATATCTGAACCTGTGACAAAAGATGAAGGCATTAACTCTTCTGAAGTTGCAGTTGCAGCCTCTGCGACTGTTCCGCCTGGTAAAAATTCAAAAGCTGCAAAGAATCCTCATGCTCCGAACAATGGTTTTTCAAAGAGCAAACCGGCCAAAGAAAAACCTACTGTGAAGGGCGCAACTCCATTCCCTCGTAAGGAGAGGGCATTGCTTTCTCAGAGTCTTTCTTTCCCGGCAAGAGGATCTCATGCAGATCCTATGAAGAAGAGCATTGATGTGTACCCAGTGAAAACAGAAATCAAACATGCTCGAGGAGCAACCAAAGCTGAGGCTCCCATTTCAGTTTCTCGTCTGAATAATCCAACTAGGCGGGCATCCACAGGAGTTCATTCAAATGATGGGAACACAACTGGAGATTCCATTAAGAGGACTTCTTTGGCATCAATACCCAGCATTAGATCCTCTTCATCCGGCAAGTCTGGTTCTGTGGATACATCCGCCAATAACCCATCTAATGGGATCCGATCAGTTGATCAAAGTTTAAATGCTGTAAAAACAACTCTGCCAATCAAAGAGGATGATGATGCTCACTCCGTTGCTTCGACTACACCTAGCGGGCGGAGGATCAGTGCTTCTGGATTTTCCTTTAGGTTGGAAGAACGTGCTGAAAAACGGAAGGAGTTTTTTACGAAGCTGGAAGAGAAGATACAGGCTAAGGAAGAAGAAAAAAATAACTCGCAAGCAAAATCAAAGGAAAGCCAGGAAGCTGAGATCAAGCGACTAAGGAAGAGCCTGACGTTTAAGGCGGCGCCCATGCCAAGTTTCTATAAAGAGCCTCCTCCGAAAGTTGAACTCAAGAAGATTCCAACCACACGGCCAAAATCTCCAAAACTTGGACGGAACAAGAGCTCCATTTCTTCGCTAAACAGTTCTTCAGAAGGTGCTGGGGCATCTCTTAGCCCACGTCTGAATCAAGAACTGAATATTTCAAAGAAAGGATTAAAGACTAAAAGCGAAAAGGACGTCATAGATCCAAAGAAGCCTATTAGAAAGTCTCAAACTAGGCTTCCTTCTCAGGAAAATGCTGCTACTAAAACTGAAGCAAAGCCTGCGAAGTCACCACCAAAGGGTACCAAAGAAGAAACACAAGACCAGAAAGCGCACACCGGAAAAACTGAAGAAGCTCAGGACCAGTCAGGGCATCTTTCCGAATTCCAAGATGAGATAGAGCCTGAAGTGAGTGTTGGCCAGATCAAAGAACCAGTCCTTGGTGCACCTATCCCGGAGATCATGCCTCATGAAGTTTCGGTTGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

492

Amino Acids

52.84

Weight (kDa)

9.46

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 266 - 340 1.1e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1182
Acc65I GGTACC 1 cut(s) 1286
AccB1I GGYRCC 2 cut(s) 964, 1286
AccB7I CCANNNNNTGG 3 cut(s) 671, 1049, 1424
AciI CCGC 6 cut(s) 236, 526, 654, 771, 775, 962
AclWI GGATC 7 cut(s) 404, 406, 609, 670, 683, 788, 1181
AcoI YGGCCR 3 cut(s) 306, 1031, 1402
AcsI RAATTY 2 cut(s) 247, 1367
AcuI CTGAAG 6 cut(s) 162, 225, 1074, 1275, 1353, 1410
AcyI GRCGYC 2 cut(s) 965, 1179
AfaI GTAC 3 cut(s) 99, 437, 1288
AfiI CCNNNNNNNGG 8 cut(s) 284, 352, 395, 582, 671, 1049, 1283, 1424
AflIII ACRYGT 1 cut(s) 82
AgsI TTSAA 5 cut(s) 252, 294, 549, 1007, 1146
AjiI CACGTC 1 cut(s) 1121
AjnI CCWGG 2 cut(s) 238, 915
AjuI GAANNNNNNNTTGG 5 cut(s) 1383, 1407, 1415, 1439, 1449
AloI GAACNNNNNNTCC 2 cut(s) 1049, 1081
AluBI AGCT 6 cut(s) 257, 482, 850, 923, 1065, 1340
AluI AGCT 6 cut(s) 257, 482, 850, 923, 1065, 1340
Alw21I GWGCWC 2 cut(s) 1067, 1432
Alw26I GTCTC 1 cut(s) 1215
Alw44I GTGCAC 1 cut(s) 1428
AlwI GGATC 7 cut(s) 404, 406, 609, 670, 683, 788, 1181
AlwNI CAGNNNCTG 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 465
AoxI GGCC 3 cut(s) 306, 1031, 1402
ApaLI GTGCAC 1 cut(s) 1428
ApeKI GCWGC 3 cut(s) 218, 257, 1241
ApoI RAATTY 2 cut(s) 247, 1367
ArsI GACNNNNNNTTYG 4 cut(s) 1164, 1196, 1404, 1436
Asp718I GGTACC 1 cut(s) 1286
AspLEI GCGC 3 cut(s) 335, 967, 1318
AspS9I GGNCC 1 cut(s) 1345
AsuC2I CCSGG 2 cut(s) 389, 1439
AsuHPI GGTGA 1 cut(s) 1266
AvaI CYCGRG 1 cut(s) 465
AvaII GGWCC 1 cut(s) 1345
BaeGI GKGCMC 1 cut(s) 1432
BalI TGGCCA 1 cut(s) 1404
BamHI GGATCC 1 cut(s) 675
BanI GGYRCC 2 cut(s) 964, 1286
BanII GRGCYC 1 cut(s) 1067
BarI GAAGNNNNNNTAC 2 cut(s) 846, 878
Bbv12I GWGCWC 2 cut(s) 1067, 1432
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 3 cut(s) 230, 244, 1228
BccI CCATC 3 cut(s) 125, 548, 673
BceAI ACGGC 2 cut(s) 137, 1046
BciT130I CCWGG 2 cut(s) 240, 917
BciVI GTATCC 1 cut(s) 640
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 2 cut(s) 389, 1439
BcoDI GTCTC 1 cut(s) 1215
BfaI CTAG 3 cut(s) 522, 768, 1218
BfoI RGCGCY 1 cut(s) 968
BfuI GTATCC 1 cut(s) 640
BisI GCNGC 4 cut(s) 219, 258, 963, 1242
BlsI GCNGC 4 cut(s) 220, 259, 964, 1243
Bme1390I CCNGG 4 cut(s) 240, 389, 917, 1439
Bme18I GGWCC 1 cut(s) 1345
BmeT110I CYCGRG 1 cut(s) 465
BmgBI CACGTC 1 cut(s) 1121
BmgT120I GGNCC 1 cut(s) 1345
BmiI GGNNCC 4 cut(s) 489, 677, 966, 1288
BmrFI CCNGG 4 cut(s) 240, 389, 917, 1439
BmrI ACTGGG 1 cut(s) 434
BmsI GCATC 5 cut(s) 539, 605, 730, 1115, 1366
BmuI ACTGGG 1 cut(s) 434
BpmI CTGGAG 1 cut(s) 588
Bpu10I CCTNAGC 3 cut(s) 483, 870, 1341
BpuEI CTTGAG 1 cut(s) 995
BpuMI CCSGG 2 cut(s) 389, 1439
BsaHI GRCGYC 2 cut(s) 965, 1179
BsaJI CCNNGG 1 cut(s) 1423
BsaWI WCCGGW 1 cut(s) 1322
BsaXI ACNNNNNCTCC 4 cut(s) 769, 799, 1049, 1079
Bsc4I CCNNNNNNNGG 8 cut(s) 284, 352, 395, 582, 671, 1049, 1283, 1424
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 4 cut(s) 440, 571, 1348, 1418
Bse3DI GCAATG 1 cut(s) 362
BseBI CCWGG 2 cut(s) 240, 917
BseDI CCNNGG 1 cut(s) 1423
BseGI GGATG 4 cut(s) 530, 623, 650, 739
BseLI CCNNNNNNNGG 8 cut(s) 284, 352, 395, 582, 671, 1049, 1283, 1424
BseMI GCAATG 1 cut(s) 362
BseMII CTCAG 5 cut(s) 386, 474, 915, 1244, 1355
BseNI ACTGG 4 cut(s) 440, 571, 1348, 1418
BseRI GAGGAG 2 cut(s) 483, 984
BseSI GKGCMC 1 cut(s) 1432
BseXI GCAGC 3 cut(s) 230, 244, 1228
BseYI CCCAGC 2 cut(s) 605, 1100
BshFI GGCC 3 cut(s) 308, 1033, 1404
BshNI GGYRCC 2 cut(s) 964, 1286
BsiHKAI GWGCWC 2 cut(s) 1067, 1432
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 5 cut(s) 305, 389, 627, 1323, 1439
BslI CCNNNNNNNGG 8 cut(s) 284, 352, 395, 582, 671, 1049, 1283, 1424
BsmAI GTCTC 1 cut(s) 1215
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 3 cut(s) 308, 1033, 1404
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 2 cut(s) 1067, 1432
BspACI CCGC 6 cut(s) 236, 526, 654, 771, 775, 962
BspANI GGCC 3 cut(s) 308, 1033, 1404
BspCNI CTCAG 5 cut(s) 385, 475, 916, 1243, 1354
BspHI TCATGA 1 cut(s) 1453
BspLI GGNNCC 4 cut(s) 489, 677, 966, 1288
BspPI GGATC 7 cut(s) 404, 406, 609, 670, 683, 788, 1181
BspQI GCTCTTC 2 cut(s) 416, 938
BspT107I GGYRCC 2 cut(s) 964, 1286
BsrDI GCAATG 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 4 cut(s) 440, 571, 1348, 1418
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 1423
BssNI GRCGYC 2 cut(s) 965, 1179
BssT1I CCWWGG 1 cut(s) 1423
Bst2UI CCWGG 2 cut(s) 240, 917
Bst4CI ACNGT 3 cut(s) 232, 325, 1085
Bst6I CTCTTC 5 cut(s) 205, 416, 625, 849, 938
BstACI GRCGYC 2 cut(s) 965, 1179
BstAPI GCANNNNNTGC 2 cut(s) 224, 1265
BstC8I GCNNGC 4 cut(s) 528, 773, 897, 1266
BstDEI CTNAG 8 cut(s) 372, 483, 870, 924, 938, 1112, 1230, 1341
BstF5I GGATG 4 cut(s) 530, 623, 650, 739
BstH2I RGCGCY 1 cut(s) 968
BstHHI GCGC 3 cut(s) 335, 967, 1318
BstMAI GTCTC 1 cut(s) 1215
BstMWI GCNNNNNNNGC 5 cut(s) 218, 224, 470, 479, 1265
BstNI CCWGG 2 cut(s) 240, 917
BstNSI RCATGY 2 cut(s) 86, 464
BstSCI CCNGG 4 cut(s) 238, 387, 915, 1437
BstSLI GKGCMC 1 cut(s) 1432
BstV1I GCAGC 3 cut(s) 230, 244, 1228
BstX2I RGATCY 5 cut(s) 398, 409, 614, 675, 1186
BstYI RGATCY 5 cut(s) 398, 409, 614, 675, 1186
BsuI GTATCC 1 cut(s) 640
BsuRI GGCC 3 cut(s) 308, 1033, 1404
BtrI CACGTC 1 cut(s) 1121
BtsCI GGATG 4 cut(s) 530, 623, 650, 739
BtsIMutI CAGTG 2 cut(s) 447, 790
Cac8I GCNNGC 4 cut(s) 528, 773, 897, 1266
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 1 cut(s) 1453
CfoI GCGC 3 cut(s) 335, 967, 1318
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 1345
Csp6I GTAC 3 cut(s) 98, 436, 1287
CviAII CATG 7 cut(s) 83, 272, 404, 461, 970, 1447, 1454
CviQI GTAC 3 cut(s) 98, 436, 1287
DdeI CTNAG 8 cut(s) 372, 483, 870, 924, 938, 1112, 1230, 1341
DinI GGCGCC 1 cut(s) 966
DraI TTTAAA 1 cut(s) 699
EaeI YGGCCR 3 cut(s) 306, 1031, 1402
Eam1104I CTCTTC 5 cut(s) 205, 416, 625, 849, 938
EarI CTCTTC 5 cut(s) 205, 416, 625, 849, 938
EciI GGCGGA 3 cut(s) 225, 643, 790
Ecl136II GAGCTC 1 cut(s) 1065
Eco130I CCWWGG 1 cut(s) 1423
Eco24I GRGCYC 1 cut(s) 1067
Eco47I GGWCC 1 cut(s) 1345
Eco53kI GAGCTC 1 cut(s) 1065
Eco57I CTGAAG 6 cut(s) 162, 225, 1074, 1275, 1353, 1410
Eco88I CYCGRG 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 1065
EcoRI GAATTC 1 cut(s) 1367
EcoRII CCWGG 2 cut(s) 238, 915
EcoT14I CCWWGG 1 cut(s) 1423
EcoT38I GRGCYC 1 cut(s) 1067
EgeI GGCGCC 1 cut(s) 966
EheI GGCGCC 1 cut(s) 966
ErhI CCWWGG 1 cut(s) 1423
FaeI CATG 7 cut(s) 86, 275, 407, 464, 973, 1450, 1457
FatI CATG 7 cut(s) 82, 271, 403, 460, 969, 1446, 1453
FauI CCCGC 2 cut(s) 519, 764
FbaI TGATCA 1 cut(s) 688
Fnu4HI GCNGC 4 cut(s) 219, 258, 963, 1242
FokI GGATG 4 cut(s) 517, 610, 637, 746
FriOI GRGCYC 1 cut(s) 1067
Fsp4HI GCNGC 4 cut(s) 219, 258, 963, 1242
FspBI CTAG 3 cut(s) 522, 768, 1218
GlaI GCGC 3 cut(s) 334, 966, 1317
GluI GCNGC 4 cut(s) 219, 258, 963, 1242
GsaI CCCAGC 2 cut(s) 609, 1104
GsuI CTGGAG 1 cut(s) 588
HaeII RGCGCY 1 cut(s) 968
HaeIII GGCC 3 cut(s) 308, 1033, 1404
HapII CCGG 5 cut(s) 305, 389, 627, 1323, 1439
HhaI GCGC 3 cut(s) 335, 967, 1318
Hin1I GRCGYC 2 cut(s) 965, 1179
Hin1II CATG 7 cut(s) 86, 275, 407, 464, 973, 1450, 1457
Hin6I GCGC 3 cut(s) 333, 965, 1316
HinP1I GCGC 3 cut(s) 333, 965, 1316
HinfI GANTC 6 cut(s) 29, 265, 376, 572, 1018, 1126
HpaII CCGG 5 cut(s) 305, 389, 627, 1323, 1439
HphI GGTGA 1 cut(s) 1266
Hpy166II GTNNAC 2 cut(s) 436, 1430
Hpy188III TCNNGA 6 cut(s) 792, 1012, 1130, 1232, 1343, 1454
Hpy8I GTNNAC 2 cut(s) 436, 1430
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 5 cut(s) 182, 322, 826, 1088, 1236
HpyCH4III ACNGT 3 cut(s) 232, 325, 1085
HpyCH4IV ACGT 4 cut(s) 817, 953, 1120, 1179
HpyCH4V TGCA 5 cut(s) 212, 218, 260, 407, 1430
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 224, 470, 479, 1265
HpyF3I CTNAG 8 cut(s) 372, 483, 870, 924, 938, 1112, 1230, 1341
HpySE526I ACGT 4 cut(s) 817, 953, 1120, 1179
Hsp92I GRCGYC 2 cut(s) 965, 1179
Hsp92II CATG 7 cut(s) 86, 275, 407, 464, 973, 1450, 1457
HspAI GCGC 3 cut(s) 333, 965, 1316
KasI GGCGCC 1 cut(s) 964
KpnI GGTACC 1 cut(s) 1290
Ksp22I TGATCA 1 cut(s) 688
LguI GCTCTTC 2 cut(s) 416, 938
LmnI GCTCC 4 cut(s) 280, 470, 493, 1070
Lsp1109I GCAGC 3 cut(s) 230, 244, 1228
LweI GCATC 5 cut(s) 539, 605, 730, 1115, 1366
MaeI CTAG 3 cut(s) 522, 768, 1218
MaeII ACGT 4 cut(s) 817, 953, 1120, 1179
MaeIII GTNAC 3 cut(s) 43, 175, 1272
MflI RGATCY 5 cut(s) 398, 409, 614, 675, 1186
MhlI GDGCHC 2 cut(s) 1067, 1432
MlsI TGGCCA 1 cut(s) 1404
MluCI AATT 2 cut(s) 247, 1367
MluNI TGGCCA 1 cut(s) 1404
Mly113I GGCGCC 1 cut(s) 965
MlyI GAGTC 2 cut(s) 38, 385
MmeI TCCRAC 4 cut(s) 542, 789, 1046, 1447
Mox20I TGGCCA 1 cut(s) 1404
MscI TGGCCA 1 cut(s) 1404
MseI TTAA 6 cut(s) 195, 579, 698, 957, 1157, 1474
MslI CAYNNNNRTG 3 cut(s) 87, 431, 549
Msp20I TGGCCA 1 cut(s) 1404
MspI CCGG 5 cut(s) 305, 389, 627, 1323, 1439
MspR9I CCNGG 4 cut(s) 240, 389, 917, 1439
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 2 cut(s) 240, 917
MwoI GCNNNNNNNGC 5 cut(s) 218, 224, 470, 479, 1265
NarI GGCGCC 1 cut(s) 965
NciI CCSGG 2 cut(s) 389, 1439
NlaIII CATG 7 cut(s) 86, 275, 407, 464, 973, 1450, 1457
NlaIV GGNNCC 4 cut(s) 489, 677, 966, 1288
NmuCI GTSAC 2 cut(s) 175, 1272
NspI RCATGY 2 cut(s) 86, 464
PaeR7I CTCGAG 1 cut(s) 465
PagI TCATGA 1 cut(s) 1453
PciI ACATGT 1 cut(s) 82
PciSI GCTCTTC 2 cut(s) 416, 938
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 4 cut(s) 265, 572, 1018, 1126
PflMI CCANNNNNTGG 3 cut(s) 671, 1049, 1424
PfoI TCCNGGA 1 cut(s) 1437
PkrI GCNGC 4 cut(s) 220, 259, 964, 1243
PleI GAGTC 2 cut(s) 37, 384
PluTI GGCGCC 1 cut(s) 968
PpsI GAGTC 2 cut(s) 37, 384
PscI ACATGT 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 1067
Psp6I CCWGG 2 cut(s) 238, 915
PspFI CCCAGC 2 cut(s) 605, 1100
PspGI CCWGG 2 cut(s) 238, 915
PspN4I GGNNCC 4 cut(s) 489, 677, 966, 1288
PspPI GGNCC 1 cut(s) 1345
PspXI VCTCGAGB 1 cut(s) 465
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 5 cut(s) 398, 409, 614, 675, 1186
RsaI GTAC 3 cut(s) 99, 437, 1288
RsaNI GTAC 3 cut(s) 98, 436, 1287
RseI CAYNNNNRTG 3 cut(s) 87, 431, 549
SacI GAGCTC 1 cut(s) 1067
SapI GCTCTTC 2 cut(s) 416, 938
SaqAI TTAA 6 cut(s) 195, 579, 698, 957, 1157, 1474
SatI GCNGC 4 cut(s) 219, 258, 963, 1242
Sau96I GGNCC 1 cut(s) 1345
SchI GAGTC 2 cut(s) 38, 385
ScrFI CCNGG 4 cut(s) 240, 389, 917, 1439
SduI GDGCHC 2 cut(s) 1067, 1432
SfaNI GCATC 5 cut(s) 539, 605, 730, 1115, 1366
SfoI GGCGCC 1 cut(s) 966
Sfr274I CTCGAG 1 cut(s) 465
SinI GGWCC 1 cut(s) 1345
SlaI CTCGAG 1 cut(s) 465
SmiMI CAYNNNNRTG 3 cut(s) 87, 431, 549
SmlI CTYRAG 2 cut(s) 465, 1010
SmoI CTYRAG 2 cut(s) 465, 1010
Sse9I AATT 2 cut(s) 247, 1367
SsiI CCGC 6 cut(s) 236, 526, 654, 771, 775, 962
SspDI GGCGCC 1 cut(s) 964
SspI AATATT 2 cut(s) 7, 1141
SspMI CTAG 3 cut(s) 522, 768, 1218
SstI GAGCTC 1 cut(s) 1067
StyD4I CCNGG 4 cut(s) 238, 387, 915, 1437
StyI CCWWGG 1 cut(s) 1423
TaaI ACNGT 3 cut(s) 232, 325, 1085
TaiI ACGT 4 cut(s) 820, 956, 1123, 1182
TaqI TCGA 5 cut(s) 11, 51, 72, 466, 758
TasI AATT 2 cut(s) 247, 1367
TauI GCSGC 1 cut(s) 965
TfiI GAWTC 4 cut(s) 265, 572, 1018, 1126
Tru1I TTAA 6 cut(s) 195, 579, 698, 957, 1157, 1474
Tru9I TTAA 6 cut(s) 195, 579, 698, 957, 1157, 1474
TscAI CASTG 2 cut(s) 447, 790
TseFI GTSAC 2 cut(s) 175, 1272
TseI GCWGC 3 cut(s) 218, 257, 1241
Tsp45I GTSAC 2 cut(s) 175, 1272
TspDTI ATGAA 6 cut(s) 17, 201, 431, 533, 612, 1470
TspGWI ACGGA 3 cut(s) 739, 844, 1069
TspRI CASTG 2 cut(s) 447, 790
Van91I CCANNNNNTGG 3 cut(s) 671, 1049, 1424
VneI GTGCAC 1 cut(s) 1428
VpaK11BI GGWCC 1 cut(s) 1345
XapI RAATTY 2 cut(s) 247, 1367
XceI RCATGY 2 cut(s) 86, 464
XhoI CTCGAG 1 cut(s) 465
XspI CTAG 3 cut(s) 522, 768, 1218
ZraI GACGTC 1 cut(s) 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.