Rroxscaffold_6G00404840

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006819
Physical Location & Seq
Reverse (-)
27337561 .. 27341555
3995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404840.1

Sequence Viewer

Length: 1470 bp
ATGGAGTCTGAAAATGGGATTGCTTTAGGGGATGAGAATTGTGCTGTTGTGGAAAAACATGTAGAAGAATCAGTACCGGATTTGAACATTGAAGGAAAGAATGTTGATGCTAATATAGAAGTTCCTACCATGAATGGAGTATCTGAACCTGTAAGCAAAGATGAAGGCATTAACCCTTCAGGAGGTGCAGTTGAAGTTGCTGCAAGTGTTCCGCTTGGTAAAAATTCAAAAACAAAAAAGGATCCTCGTGCTCCAAACAATGGTGTTTCAAAGGGAAAGTTGGCCAAGGATAAACCCAGTGTGAAAGGTGCAACTCCAGTTTCTCGTACACAAAGGGCAATACTTTCTCAGAGTCTTTCGTTCCCAGCCAGGGGAGCTCGTGTTGATGCTATGAAGAAGAGCATTGATGTTTATCCAGTGAAAACAGAAGTCAAACACACTCGAGGGAATGCAACCAAAGCTGAGGCCCCTTTTCCATCTTCTCGTTTGAATCATCAAAATAGGCGTGCATCCACAGGAATACACTCAAATGATGAGAACAAAGCTGGTGGAGCTTCAGTTAGGAGGACTACTTTAGCATCAATACCTAGCATGAGATCCTCTGCATCAATGAAGTCTGGCTCTGTGAGTAAACCTGCTAATAGTTCATATGATGTGACCCGATCAGTTGGGCAGATCTTAAGTGCTGTAAAAACAACCCTGCCAATCAAAGAGGATGATGATGCTCATTCCATTGCTTCAAGCACTACACCTGGTGGGCGGAGGAGCAGTGGTTCTGCATTTTCCTTCAGGTTGGATGAACAAGAGAAGATACAGGCTAAGGAAGCAGAAAAAAATAATTCGCAAGCAAAATCAAAGGAAAACCAGGAAGCAGAGATCAAGCAACTGAGGAAGAGCCTGACATTTAGGGCAGCTCCCATGCCAACTTTCTATAAAGAGCCTCCTCCAAAAATTGAAATAAAGAAGATACCGACCACACGTCCCATATCTCCAAAGCTTGGAAGGCACAAGAACTCCATTTCTTCATTGAACAACCCCTCAGAAGGTGGTGGGACGTGTCTTAGCCCGCGTCTGAATCAAGAACAGAACAATTCAAACAAAGGGTTAAAGGCAACAGGTGAAAAAGATGTCACAGAATCAAAGAAGCCTATCAGGAAGTCCCAACATCGGCTTCATTCTCAGGAAAATGTGGCTGCTAAAGCTGAAGGAAAGCCCTGTAAGTCACAACTGAAAGTGGCCAGCAAAGAAGAAAAAGAAAGCCCGAAGGCATGCACTGGAGAGACAGAAGAAAGCTTCCAAGACCAATCTGAACATCTTTCTGAATGCAAACATAAGATAGAGCCTGAAGTGAGTGTTGACCAGACCAAAGAACCAGTTGTCAGTGCAGCTCCTGCTCCTGAGATCATGCCTCAGGAAGTTACCATTGCAGCTCCTGAGATCATGCCTCGAGGAGTTGCCATTGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

489

Amino Acids

52.4

Weight (kDa)

9.33

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 268 - 318 7.4e-15 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1446
Acc36I ACCTGC 1 cut(s) 643
AccB7I CCANNNNNTGG 2 cut(s) 260, 998
AccII CGCG 1 cut(s) 1069
AciI CCGC 3 cut(s) 212, 760, 1067
AclWI GGATC 3 cut(s) 236, 249, 591
AcoI YGGCCR 2 cut(s) 282, 1236
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 5 cut(s) 162, 540, 772, 1224, 1365
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 2 cut(s) 75, 328
AfiI CCNNNNNNNGG 7 cut(s) 182, 260, 370, 371, 998, 1043, 1167
AflII CTTAAG 1 cut(s) 679
AflIII ACRYGT 3 cut(s) 58, 977, 1055
AhdI GACNNNNNGTC 1 cut(s) 978
AjiI CACGTC 2 cut(s) 980, 1056
AjnI CCWGG 3 cut(s) 368, 751, 864
AjuI GAANNNNNNNTTGG 2 cut(s) 1155, 1187
AloI GAACNNNNNNTCC 2 cut(s) 757, 789
Alw21I GWGCWC 2 cut(s) 253, 379
Alw26I GTCTC 1 cut(s) 1274
AlwI GGATC 3 cut(s) 236, 249, 591
AlwNI CAGNNNCTG 2 cut(s) 1391, 1433
Ama87I CYCGRG 2 cut(s) 441, 1446
AoxI GGCC 3 cut(s) 282, 465, 1236
ApeKI GCWGC 5 cut(s) 200, 911, 1193, 1385, 1427
ApoI RAATTY 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 1130
AvaI CYCGRG 2 cut(s) 441, 1446
AxyI CCTNAGG 1 cut(s) 1410
BalI TGGCCA 2 cut(s) 284, 1238
BamHI GGATCC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 379
BarI GAAGNNNNNNTAC 2 cut(s) 57, 89
BauI CACGAG 2 cut(s) 246, 378
Bbv12I GWGCWC 2 cut(s) 253, 379
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 5 cut(s) 187, 923, 1180, 1397, 1439
BccI CCATC 1 cut(s) 484
BciT130I CCWGG 3 cut(s) 370, 753, 866
BcoDI GTCTC 1 cut(s) 1274
BfaI CTAG 1 cut(s) 588
BfrI CTTAAG 1 cut(s) 679
BfuAI ACCTGC 1 cut(s) 643
BglII AGATCT 1 cut(s) 675
BisI GCNGC 5 cut(s) 201, 912, 1194, 1386, 1428
BlsI GCNGC 5 cut(s) 202, 913, 1195, 1387, 1429
Bme1390I CCNGG 3 cut(s) 370, 753, 866
BmeRI GACNNNNNGTC 1 cut(s) 978
BmeT110I CYCGRG 2 cut(s) 441, 1446
BmgBI CACGTC 2 cut(s) 980, 1056
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 2 cut(s) 243, 468
BmrFI CCNGG 3 cut(s) 370, 753, 866
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
BmuI ACTGGG 1 cut(s) 291
BpmI CTGGAG 2 cut(s) 300, 1296
Bpu10I CCTNAGC 2 cut(s) 462, 819
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 2 cut(s) 285, 369
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 7 cut(s) 182, 260, 370, 371, 998, 1043, 1167
Bse1I ACTGG 5 cut(s) 297, 317, 416, 1279, 1373
Bse21I CCTNAGG 1 cut(s) 1410
Bse3DI GCAATG 2 cut(s) 732, 1422
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 3 cut(s) 370, 753, 866
BseDI CCNNGG 2 cut(s) 285, 369
BseGI GGATG 4 cut(s) 37, 509, 721, 802
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 7 cut(s) 182, 260, 370, 371, 998, 1043, 1167
BseMI GCAATG 2 cut(s) 732, 1422
BseMII CTCAG 8 cut(s) 362, 453, 878, 1053, 1193, 1389, 1424, 1425
BseNI ACTGG 5 cut(s) 297, 317, 416, 1279, 1373
BseRI GAGGAG 3 cut(s) 778, 933, 1464
BseXI GCAGC 5 cut(s) 187, 923, 1180, 1397, 1439
BseYI CCCAGC 1 cut(s) 364
BsgI GTGCAG 2 cut(s) 207, 1404
Bsh1236I CGCG 1 cut(s) 1069
BshFI GGCC 3 cut(s) 284, 467, 1238
BsiHKAI GWGCWC 2 cut(s) 253, 379
BsiHKCI CYCGRG 2 cut(s) 441, 1446
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 3 cut(s) 966, 1066, 1144
BslI CCNNNNNNNGG 7 cut(s) 182, 260, 370, 371, 998, 1043, 1167
BsmAI GTCTC 1 cut(s) 1274
BsmFI GGGAC 3 cut(s) 966, 1066, 1144
BsmI GAATGC 2 cut(s) 454, 1328
BsnI GGCC 3 cut(s) 284, 467, 1238
BsoBI CYCGRG 2 cut(s) 441, 1446
Bsp1286I GDGCHC 2 cut(s) 253, 379
Bsp143I GATC 7 cut(s) 241, 596, 662, 675, 876, 1401, 1437
BspACI CCGC 3 cut(s) 212, 760, 1067
BspANI GGCC 3 cut(s) 284, 467, 1238
BspCNI CTCAG 8 cut(s) 361, 454, 879, 1052, 1192, 1390, 1423, 1426
BspFNI CGCG 1 cut(s) 1069
BspLI GGNNCC 2 cut(s) 243, 468
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 3 cut(s) 236, 249, 591
BspQI GCTCTTC 2 cut(s) 392, 887
BspTI CTTAAG 1 cut(s) 679
BsrDI GCAATG 2 cut(s) 732, 1422
BsrI ACTGG 5 cut(s) 297, 317, 416, 1279, 1373
BssECI CCNNGG 2 cut(s) 285, 369
BssMI GATC 7 cut(s) 241, 596, 662, 675, 876, 1401, 1437
BssSI CACGAG 2 cut(s) 246, 378
BssT1I CCWWGG 1 cut(s) 285
Bst2BI CACGAG 2 cut(s) 246, 378
Bst2UI CCWGG 3 cut(s) 370, 753, 866
Bst6I CTCTTC 2 cut(s) 392, 887
BstAFI CTTAAG 1 cut(s) 679
BstAPI GCANNNNNTGC 1 cut(s) 1391
BstC8I GCNNGC 5 cut(s) 507, 846, 1067, 1240, 1270
BstENI CCTNNNNNAGG 1 cut(s) 180
BstF5I GGATG 4 cut(s) 37, 509, 721, 802
BstFNI CGCG 1 cut(s) 1069
BstKTI GATC 7 cut(s) 244, 599, 665, 678, 879, 1404, 1440
BstMAI GTCTC 1 cut(s) 1274
BstMBI GATC 7 cut(s) 241, 596, 662, 675, 876, 1401, 1437
BstMWI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 824, 1003, 1199, 1391
BstNI CCWGG 3 cut(s) 370, 753, 866
BstNSI RCATGY 2 cut(s) 62, 1272
BstSCI CCNGG 3 cut(s) 368, 751, 864
BstUI CGCG 1 cut(s) 1069
BstV1I GCAGC 5 cut(s) 187, 923, 1180, 1397, 1439
BstX2I RGATCY 3 cut(s) 241, 596, 675
BstYI RGATCY 3 cut(s) 241, 596, 675
Bsu36I CCTNAGG 1 cut(s) 1410
BsuRI GGCC 3 cut(s) 284, 467, 1238
BtrI CACGTC 2 cut(s) 980, 1056
BtsCI GGATG 4 cut(s) 37, 509, 721, 802
BtsI GCAGTG 1 cut(s) 775
BtsIMutI CAGTG 5 cut(s) 304, 423, 775, 1272, 1387
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 5 cut(s) 507, 846, 1067, 1240, 1270
CaiI CAGNNNCTG 2 cut(s) 1391, 1433
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 1 cut(s) 1058
CsiI ACCWGGT 1 cut(s) 751
Csp6I GTAC 2 cut(s) 74, 327
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviAII CATG 7 cut(s) 59, 130, 592, 919, 1269, 1405, 1441
CviQI GTAC 2 cut(s) 74, 327
DpnI GATC 7 cut(s) 243, 598, 664, 677, 878, 1403, 1439
DpnII GATC 7 cut(s) 241, 596, 662, 675, 876, 1401, 1437
DraIII CACNNNGTG 1 cut(s) 755
DriI GACNNNNNGTC 1 cut(s) 978
EaeI YGGCCR 2 cut(s) 282, 1236
Eam1104I CTCTTC 2 cut(s) 392, 887
Eam1105I GACNNNNNGTC 1 cut(s) 978
EarI CTCTTC 2 cut(s) 392, 887
EciI GGCGGA 1 cut(s) 775
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 377
Eco57I CTGAAG 5 cut(s) 162, 540, 772, 1224, 1365
Eco81I CCTNAGG 1 cut(s) 1410
Eco88I CYCGRG 2 cut(s) 441, 1446
EcoICRI GAGCTC 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 3 cut(s) 368, 751, 864
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 7 cut(s) 62, 133, 595, 922, 1272, 1408, 1444
FaqI GGGAC 3 cut(s) 966, 1066, 1144
FatI CATG 7 cut(s) 58, 129, 591, 918, 1268, 1404, 1440
FauI CCCGC 1 cut(s) 1074
FauNDI CATATG 1 cut(s) 649
Fnu4HI GCNGC 5 cut(s) 201, 912, 1194, 1386, 1428
FokI GGATG 4 cut(s) 44, 496, 728, 809
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 5 cut(s) 201, 912, 1194, 1386, 1428
FspBI CTAG 1 cut(s) 588
GluI GCNGC 5 cut(s) 201, 912, 1194, 1386, 1428
GsaI CCCAGC 1 cut(s) 368
GsuI CTGGAG 2 cut(s) 300, 1296
HaeIII GGCC 3 cut(s) 284, 467, 1238
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 1058
Hin1II CATG 7 cut(s) 62, 133, 595, 922, 1272, 1408, 1444
HincII GTYRAC 1 cut(s) 1357
HindII GTYRAC 1 cut(s) 1357
HindIII AAGCTT 2 cut(s) 995, 1291
HinfI GANTC 6 cut(s) 5, 68, 352, 490, 1075, 1136
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 1130
Hpy166II GTNNAC 3 cut(s) 329, 632, 1357
Hpy188I TCNGA 7 cut(s) 10, 145, 351, 1042, 1074, 1309, 1321
Hpy188III TCNNGA 7 cut(s) 180, 1079, 1153, 1181, 1397, 1412, 1433
Hpy8I GTNNAC 3 cut(s) 329, 632, 1357
HpyAV CCTTC 8 cut(s) 86, 158, 186, 796, 996, 1037, 1199, 1258
HpyCH4IV ACGT 2 cut(s) 979, 1055
HpyF10VI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 824, 1003, 1199, 1391
HpySE526I ACGT 2 cut(s) 979, 1055
Hsp92II CATG 7 cut(s) 62, 133, 595, 922, 1272, 1408, 1444
Kzo9I GATC 7 cut(s) 241, 596, 662, 675, 876, 1401, 1437
LguI GCTCTTC 2 cut(s) 392, 887
LmnI GCTCC 8 cut(s) 256, 374, 551, 765, 919, 1393, 1399, 1435
Lsp1109I GCAGC 5 cut(s) 187, 923, 1180, 1397, 1439
LweI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
MabI ACCWGGT 1 cut(s) 751
MaeI CTAG 1 cut(s) 588
MaeII ACGT 2 cut(s) 979, 1055
MaeIII GTNAC 4 cut(s) 655, 1129, 1221, 1417
MalI GATC 7 cut(s) 243, 598, 664, 677, 878, 1403, 1439
MboI GATC 7 cut(s) 241, 596, 662, 675, 876, 1401, 1437
MflI RGATCY 3 cut(s) 241, 596, 675
MhlI GDGCHC 2 cut(s) 253, 379
MlsI TGGCCA 2 cut(s) 284, 1238
MluCI AATT 5 cut(s) 37, 223, 838, 951, 1090
MluNI TGGCCA 2 cut(s) 284, 1238
MlyI GAGTC 2 cut(s) 14, 361
MmeI TCCRAC 1 cut(s) 774
Mox20I TGGCCA 2 cut(s) 284, 1238
MscI TGGCCA 2 cut(s) 284, 1238
MseI TTAA 4 cut(s) 171, 680, 1106, 1468
MslI CAYNNNNRTG 1 cut(s) 528
Msp20I TGGCCA 2 cut(s) 284, 1238
MspCI CTTAAG 1 cut(s) 679
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 3 cut(s) 370, 753, 866
Mva1269I GAATGC 2 cut(s) 454, 1328
MvaI CCWGG 3 cut(s) 370, 753, 866
MvnI CGCG 1 cut(s) 1069
MwoI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 824, 1003, 1199, 1391
NdeI CATATG 1 cut(s) 649
NdeII GATC 7 cut(s) 241, 596, 662, 675, 876, 1401, 1437
NlaIII CATG 7 cut(s) 62, 133, 595, 922, 1272, 1408, 1444
NlaIV GGNNCC 2 cut(s) 243, 468
NmuCI GTSAC 3 cut(s) 655, 1129, 1221
NspI RCATGY 2 cut(s) 62, 1272
PaeI GCATGC 1 cut(s) 1272
PaeR7I CTCGAG 2 cut(s) 441, 1446
PciI ACATGT 1 cut(s) 58
PciSI GCTCTTC 2 cut(s) 392, 887
PctI GAATGC 2 cut(s) 454, 1328
PfeI GAWTC 4 cut(s) 68, 490, 1075, 1136
PflMI CCANNNNNTGG 2 cut(s) 260, 998
PkrI GCNGC 5 cut(s) 202, 913, 1195, 1387, 1429
PleI GAGTC 2 cut(s) 13, 360
PpsI GAGTC 2 cut(s) 13, 360
PscI ACATGT 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 368, 751, 864
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 3 cut(s) 368, 751, 864
PspN4I GGNNCC 2 cut(s) 243, 468
PspPI GGNCC 1 cut(s) 466
PspXI VCTCGAGB 2 cut(s) 441, 1446
PstNI CAGNNNCTG 2 cut(s) 1391, 1433
PsuI RGATCY 3 cut(s) 241, 596, 675
RsaI GTAC 2 cut(s) 75, 328
RsaNI GTAC 2 cut(s) 74, 327
RseI CAYNNNNRTG 1 cut(s) 528
SacI GAGCTC 1 cut(s) 379
SapI GCTCTTC 2 cut(s) 392, 887
SaqAI TTAA 4 cut(s) 171, 680, 1106, 1468
SatI GCNGC 5 cut(s) 201, 912, 1194, 1386, 1428
Sau3AI GATC 7 cut(s) 241, 596, 662, 675, 876, 1401, 1437
Sau96I GGNCC 1 cut(s) 466
SchI GAGTC 2 cut(s) 14, 361
ScrFI CCNGG 3 cut(s) 370, 753, 866
SduI GDGCHC 2 cut(s) 253, 379
SexAI ACCWGGT 1 cut(s) 751
SfaNI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
Sfr274I CTCGAG 2 cut(s) 441, 1446
SlaI CTCGAG 2 cut(s) 441, 1446
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 3 cut(s) 441, 679, 1446
SmoI CTYRAG 3 cut(s) 441, 679, 1446
SphI GCATGC 1 cut(s) 1272
Sse9I AATT 5 cut(s) 37, 223, 838, 951, 1090
SsiI CCGC 3 cut(s) 212, 760, 1067
SspMI CTAG 1 cut(s) 588
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 3 cut(s) 368, 751, 864
StyI CCWWGG 1 cut(s) 285
TaiI ACGT 2 cut(s) 982, 1058
TaqI TCGA 2 cut(s) 442, 1447
TasI AATT 5 cut(s) 37, 223, 838, 951, 1090
TfiI GAWTC 4 cut(s) 68, 490, 1075, 1136
Tru1I TTAA 4 cut(s) 171, 680, 1106, 1468
Tru9I TTAA 4 cut(s) 171, 680, 1106, 1468
TscAI CASTG 5 cut(s) 304, 423, 775, 1279, 1387
TseFI GTSAC 3 cut(s) 655, 1129, 1221
TseI GCWGC 5 cut(s) 200, 911, 1193, 1385, 1427
Tsp45I GTSAC 3 cut(s) 655, 1129, 1221
TspDTI ATGAA 8 cut(s) 146, 177, 407, 626, 636, 813, 1014, 1163
TspRI CASTG 5 cut(s) 304, 423, 775, 1279, 1387
Van91I CCANNNNNTGG 2 cut(s) 260, 998
Vha464I CTTAAG 1 cut(s) 679
XagI CCTNNNNNAGG 1 cut(s) 180
XapI RAATTY 1 cut(s) 223
XceI RCATGY 2 cut(s) 62, 1272
XhoI CTCGAG 2 cut(s) 441, 1446
XspI CTAG 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.