Rorug03G0158600

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002410
Physical Location & Seq
Reverse (-)
13076864 .. 13080394
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0158600.1

Sequence Viewer

Length: 996 bp
ATGGCTTCCTATAAATATAAGCAGCACTCTTTGGTTTTAAGCTTCGTGATCACGTTATTTCTGTTGCTCTTGCAATTTCAAGTTGGTATAAGTGAGCTGCAACAGAACTATTATTCCCAGAGTTGCCCTAAAGCTGAAGAAATCATCAAGCAAGAGGTCACTAACCTTTACGAGGAACATGGAAATACCGCCGTCTCCTGGATCAGAAATCTCTTCCACGATTGCATTGTTGAGGGATGTGATGCGTCTCTGTTGTTAGAAGCGGCAGACGGCATGGAGTCAGAGATAGCGTCGGATCGGAGCTTTGGGATGAGAAATTTTAAGTACGTGAACACCATCAAACAGGCCCTAGAGAAGGAATGTCCTCTTACAGTTTCTTGCGCTGATGTTATAACTCTTTCTGCAAGAGATGGCATTGTCATGTTAGGAGGGCCTCGGATTGAAATGAAAACGGGAAGGAGAGACAGCAAGGAGAGTCATGCAAGAGTGGTGGATGAGTCTATACCTAATCACAATGATTCCATCTCCTTAGTGCTCTCTCGATTTCAATCGATCGGCATCGATGTTGAAGGCATGGTTGCCCTTTTAGGAGCTCACTCCGTGGGTCGAGTTCACTGCCTGAATCTTGTGCACAGACTCTACCCTACTGTTGATCCAACTTTGGATCCCGACCATGCTGCGTACCTCAAAGTTCGCTGCCCGACTCCAATTCCTGACCCGAAAGCAGTGCAGTATTCAAGATTCGACCGCGAAACACCCATGATTCTGGATAACATGTACTACAAGAACGTCTTGAACCACAAGGGTTTGCTCATAATCGATCAACAACTGAGTTCTGACCCAGAGACATTGCCTTTTGTGGAGAAGATGGCTTCCGATAATGGATACTTCTACGAGCAGTTTTCGAGAGCTGCAATTCTTTTGTCGGAGAACAATCCACTTACTGGAGACCAAGGAGAGATCAGAAAGGATTGCCGCTATGTGAACGGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

331

Amino Acids

37.24

Weight (kDa)

5.4

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 49 - 293 2.4e-70 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AccB7I CCANNNNNTGG 1 cut(s) 944
AccII CGCG 1 cut(s) 750
AciI CCGC 4 cut(s) 189, 263, 748, 976
AclWI GGATC 5 cut(s) 209, 303, 647, 659, 672
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 1 cut(s) 156
AdeI CACNNNGTG 1 cut(s) 601
AfaI GTAC 3 cut(s) 326, 683, 779
AfiI CCNNNNNNNGG 4 cut(s) 172, 198, 355, 944
AflIII ACRYGT 1 cut(s) 774
AgsI TTSAA 6 cut(s) 80, 443, 548, 569, 738, 796
AjnI CCWGG 1 cut(s) 197
AloI GAACNNNNNNTCC 2 cut(s) 98, 130
AluBI AGCT 6 cut(s) 42, 97, 134, 303, 593, 911
AluI AGCT 6 cut(s) 42, 97, 134, 303, 593, 911
Alw21I GWGCWC 3 cut(s) 537, 595, 633
Alw26I GTCTC 5 cut(s) 199, 252, 456, 839, 942
Alw44I GTGCAC 1 cut(s) 629
AlwI GGATC 5 cut(s) 209, 303, 647, 659, 672
AoxI GGCC 2 cut(s) 345, 431
ApaLI GTGCAC 1 cut(s) 629
ApeKI GCWGC 5 cut(s) 22, 97, 677, 696, 911
ApoI RAATTY 1 cut(s) 316
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 346, 431
BaeGI GKGCMC 1 cut(s) 633
BamHI GGATCC 1 cut(s) 664
BanII GRGCYC 1 cut(s) 595
Bbv12I GWGCWC 3 cut(s) 537, 595, 633
BbvI GCAGC 5 cut(s) 34, 84, 664, 683, 898
BccI CCATC 4 cut(s) 344, 404, 530, 862
BceAI ACGGC 2 cut(s) 176, 286
BcgI CGANNNNNNTGC 6 cut(s) 597, 631, 659, 693, 709, 743
BciT130I CCWGG 1 cut(s) 199
BciVI GTATCC 1 cut(s) 878
BclI TGATCA 1 cut(s) 48
BcoDI GTCTC 5 cut(s) 199, 252, 456, 839, 942
BfaI CTAG 1 cut(s) 350
BfuI GTATCC 1 cut(s) 878
BisI GCNGC 7 cut(s) 23, 98, 264, 678, 697, 912, 976
BlsI GCNGC 7 cut(s) 24, 99, 265, 679, 698, 913, 977
Bme1390I CCNGG 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 346, 431
BmiI GGNNCC 1 cut(s) 666
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 2 cut(s) 232, 567
BpmI CTGGAG 1 cut(s) 966
Bsa29I ATCGAT 3 cut(s) 551, 561, 819
BsaAI YACGTR 1 cut(s) 328
BsaBI GATNNNNATC 2 cut(s) 547, 557
BsaI GGTCTC 1 cut(s) 942
BsaJI CCNNGG 3 cut(s) 434, 600, 952
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 198, 355, 944
Bse1I ACTGG 1 cut(s) 949
Bse3DI GCAATG 1 cut(s) 848
Bse8I GATNNNNATC 2 cut(s) 547, 557
BseBI CCWGG 1 cut(s) 199
BseCI ATCGAT 3 cut(s) 551, 561, 819
BseDI CCNNGG 3 cut(s) 434, 600, 952
BseGI GGATG 3 cut(s) 242, 315, 499
BseJI GATNNNNATC 2 cut(s) 547, 557
BseLI CCNNNNNNNGG 4 cut(s) 172, 198, 355, 944
BseMI GCAATG 1 cut(s) 848
BseMII CTCAG 1 cut(s) 821
BseNI ACTGG 1 cut(s) 949
BseSI GKGCMC 1 cut(s) 633
BseXI GCAGC 5 cut(s) 34, 84, 664, 683, 898
BsgI GTGCAG 1 cut(s) 749
Bsh1236I CGCG 1 cut(s) 750
Bsh1285I CGRYCG 2 cut(s) 555, 748
BshFI GGCC 2 cut(s) 347, 433
BshVI ATCGAT 3 cut(s) 551, 561, 819
BsiEI CGRYCG 2 cut(s) 555, 748
BsiHKAI GWGCWC 3 cut(s) 537, 595, 633
BslI CCNNNNNNNGG 4 cut(s) 172, 198, 355, 944
BsmAI GTCTC 5 cut(s) 199, 252, 456, 839, 942
BsmBI CGTCTC 2 cut(s) 199, 252
BsnI GGCC 2 cut(s) 347, 433
Bso31I GGTCTC 1 cut(s) 942
Bsp1286I GDGCHC 3 cut(s) 537, 595, 633
Bsp143I GATC 8 cut(s) 48, 201, 295, 552, 652, 664, 820, 960
BspACI CCGC 4 cut(s) 189, 263, 748, 976
BspANI GGCC 2 cut(s) 347, 433
BspCNI CTCAG 1 cut(s) 822
BspDI ATCGAT 3 cut(s) 551, 561, 819
BspFNI CGCG 1 cut(s) 750
BspLI GGNNCC 1 cut(s) 666
BspPI GGATC 5 cut(s) 209, 303, 647, 659, 672
BspTNI GGTCTC 1 cut(s) 942
BsrDI GCAATG 1 cut(s) 848
BsrI ACTGG 1 cut(s) 949
BssECI CCNNGG 3 cut(s) 434, 600, 952
BssMI GATC 8 cut(s) 48, 201, 295, 552, 652, 664, 820, 960
BssT1I CCWWGG 1 cut(s) 952
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 2 cut(s) 373, 649
Bst6I CTCTTC 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 328
BstDEI CTNAG 2 cut(s) 529, 830
BstDSI CCRYGG 1 cut(s) 600
BstENI CCTNNNNNAGG 2 cut(s) 170, 353
BstF5I GGATG 3 cut(s) 242, 315, 499
BstFNI CGCG 1 cut(s) 750
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 8 cut(s) 51, 204, 298, 555, 655, 667, 823, 963
BstMAI GTCTC 5 cut(s) 199, 252, 456, 839, 942
BstMBI GATC 8 cut(s) 48, 201, 295, 552, 652, 664, 820, 960
BstMCI CGRYCG 2 cut(s) 555, 748
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 1 cut(s) 778
BstSCI CCNGG 1 cut(s) 197
BstSLI GKGCMC 1 cut(s) 633
BstUI CGCG 1 cut(s) 750
BstV1I GCAGC 5 cut(s) 34, 84, 664, 683, 898
BstX2I RGATCY 1 cut(s) 664
BstXI CCANNNNNNTGG 1 cut(s) 766
BstYI RGATCY 1 cut(s) 664
Bsu15I ATCGAT 3 cut(s) 551, 561, 819
BsuI GTATCC 1 cut(s) 878
BsuRI GGCC 2 cut(s) 347, 433
BsuTUI ATCGAT 3 cut(s) 551, 561, 819
BtgI CCRYGG 1 cut(s) 600
BtsCI GGATG 3 cut(s) 242, 315, 499
BtsI GCAGTG 2 cut(s) 613, 732
BtsIMutI CAGTG 2 cut(s) 613, 732
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 346, 431
ClaI ATCGAT 3 cut(s) 551, 561, 819
CseI GACGC 2 cut(s) 234, 279
Csp6I GTAC 3 cut(s) 325, 682, 778
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CviAII CATG 8 cut(s) 179, 274, 421, 479, 574, 674, 760, 775
CviQI GTAC 3 cut(s) 325, 682, 778
DdeI CTNAG 2 cut(s) 529, 830
DpnI GATC 8 cut(s) 50, 203, 297, 554, 654, 666, 822, 962
DpnII GATC 8 cut(s) 48, 201, 295, 552, 652, 664, 820, 960
DraIII CACNNNGTG 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Ecl136II GAGCTC 1 cut(s) 593
Eco130I CCWWGG 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 595
Eco31I GGTCTC 1 cut(s) 942
Eco53kI GAGCTC 1 cut(s) 593
Eco57I CTGAAG 1 cut(s) 156
EcoICRI GAGCTC 1 cut(s) 593
EcoNI CCTNNNNNAGG 2 cut(s) 170, 353
EcoO109I RGGNCCY 2 cut(s) 346, 431
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 1 cut(s) 952
EcoT38I GRGCYC 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 952
Esp3I CGTCTC 2 cut(s) 199, 252
FaeI CATG 8 cut(s) 182, 277, 424, 482, 577, 677, 763, 778
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FatI CATG 8 cut(s) 178, 273, 420, 478, 573, 673, 759, 774
FbaI TGATCA 1 cut(s) 48
Fnu4HI GCNGC 7 cut(s) 23, 98, 264, 678, 697, 912, 976
FokI GGATG 3 cut(s) 249, 322, 506
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 7 cut(s) 23, 98, 264, 678, 697, 912, 976
FspBI CTAG 1 cut(s) 350
GlaI GCGC 1 cut(s) 382
GluI GCNGC 7 cut(s) 23, 98, 264, 678, 697, 912, 976
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 2 cut(s) 347, 433
HgaI GACGC 2 cut(s) 234, 279
HhaI GCGC 1 cut(s) 383
Hin1II CATG 8 cut(s) 182, 277, 424, 482, 577, 677, 763, 778
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 9 cut(s) 278, 475, 497, 518, 622, 636, 703, 741, 763
Hpy166II GTNNAC 4 cut(s) 331, 613, 631, 985
Hpy188I TCNGA 9 cut(s) 206, 283, 295, 300, 438, 838, 877, 928, 965
Hpy188III TCNNGA 8 cut(s) 46, 540, 668, 713, 738, 767, 793, 906
Hpy8I GTNNAC 4 cut(s) 331, 613, 631, 985
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 3 cut(s) 349, 450, 563
HpyCH4III ACNGT 2 cut(s) 373, 649
HpyCH4IV ACGT 3 cut(s) 53, 327, 789
HpyCH4V TGCA 8 cut(s) 73, 100, 225, 404, 482, 631, 730, 914
HpyF3I CTNAG 2 cut(s) 529, 830
HpySE526I ACGT 3 cut(s) 53, 327, 789
Hsp92II CATG 8 cut(s) 182, 277, 424, 482, 577, 677, 763, 778
HspAI GCGC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 8 cut(s) 48, 201, 295, 552, 652, 664, 820, 960
LmnI GCTCC 2 cut(s) 300, 590
LpnPI CCDG 9 cut(s) 131, 184, 211, 329, 632, 726, 752, 855, 930
Lsp1109I GCAGC 5 cut(s) 34, 84, 664, 683, 898
LweI GCATC 2 cut(s) 232, 567
MaeI CTAG 1 cut(s) 350
MaeII ACGT 3 cut(s) 53, 327, 789
MaeIII GTNAC 1 cut(s) 157
MalI GATC 8 cut(s) 50, 203, 297, 554, 654, 666, 822, 962
MboI GATC 8 cut(s) 48, 201, 295, 552, 652, 664, 820, 960
MboII GAAGA 3 cut(s) 149, 205, 877
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 3 cut(s) 537, 595, 633
MluCI AATT 4 cut(s) 74, 316, 708, 915
MlyI GAGTC 5 cut(s) 287, 484, 506, 630, 697
MmeI TCCRAC 3 cut(s) 273, 680, 906
MnlI CCTC 7 cut(s) 148, 166, 226, 375, 422, 444, 695
MseI TTAA 2 cut(s) 38, 321
MslI CAYNNNNRTG 1 cut(s) 419
MspR9I CCNGG 1 cut(s) 199
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 1 cut(s) 750
NdeII GATC 8 cut(s) 48, 201, 295, 552, 652, 664, 820, 960
NlaIII CATG 8 cut(s) 182, 277, 424, 482, 577, 677, 763, 778
NlaIV GGNNCC 1 cut(s) 666
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 778
PciI ACATGT 1 cut(s) 774
PfeI GAWTC 4 cut(s) 518, 622, 741, 763
PflMI CCANNNNNTGG 1 cut(s) 944
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 7 cut(s) 24, 99, 265, 679, 698, 913, 977
Ple19I CGATCG 1 cut(s) 555
PleI GAGTC 5 cut(s) 286, 483, 505, 630, 697
PpsI GAGTC 5 cut(s) 286, 483, 505, 630, 697
Ppu21I YACGTR 1 cut(s) 328
PscI ACATGT 1 cut(s) 774
PsiI TTATAA 1 cut(s) 392
Psp124BI GAGCTC 1 cut(s) 595
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 666
PspPI GGNCC 2 cut(s) 346, 431
PsuI RGATCY 1 cut(s) 664
PvuI CGATCG 1 cut(s) 555
RsaI GTAC 3 cut(s) 326, 683, 779
RsaNI GTAC 3 cut(s) 325, 682, 778
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 595
SaqAI TTAA 2 cut(s) 38, 321
SatI GCNGC 7 cut(s) 23, 98, 264, 678, 697, 912, 976
Sau3AI GATC 8 cut(s) 48, 201, 295, 552, 652, 664, 820, 960
Sau96I GGNCC 2 cut(s) 346, 431
SchI GAGTC 5 cut(s) 287, 484, 506, 630, 697
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 3 cut(s) 537, 595, 633
SfaNI GCATC 2 cut(s) 232, 567
SmiMI CAYNNNNRTG 1 cut(s) 419
Sse9I AATT 4 cut(s) 74, 316, 708, 915
SsiI CCGC 4 cut(s) 189, 263, 748, 976
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 1 cut(s) 595
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 1 cut(s) 952
TaaI ACNGT 2 cut(s) 373, 649
TaiI ACGT 3 cut(s) 56, 330, 792
TaqI TCGA 7 cut(s) 541, 551, 561, 607, 744, 819, 905
TasI AATT 4 cut(s) 74, 316, 708, 915
TatI WGTACW 1 cut(s) 777
TauI GCSGC 2 cut(s) 266, 978
TfiI GAWTC 4 cut(s) 518, 622, 741, 763
Tru1I TTAA 2 cut(s) 38, 321
Tru9I TTAA 2 cut(s) 38, 321
TscAI CASTG 2 cut(s) 620, 732
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 5 cut(s) 22, 97, 677, 696, 911
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 461
TspGWI ACGGA 1 cut(s) 589
TspRI CASTG 2 cut(s) 620, 732
Van91I CCANNNNNTGG 1 cut(s) 944
VneI GTGCAC 1 cut(s) 629
XagI CCTNNNNNAGG 2 cut(s) 170, 353
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 778
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.