Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002410

Physical Location & Seq

Genomic Coordinates Forward (+)

4960581 .. 4964287

Gene Structure 3707 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1044400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGGGGAACAACTGCGTCGGATCAACGAAGAACACACTCATCCATTCAGTCTCGAATTCACTCTGGTGGTCTCGATCAACGGGCGGTTTTTCCAGTAGGGGAATCAATAGTACTGATGAAATGCCGCAATTGAGTAAGAATCCGTCAGAAGCTACTCTTTATGTTCAAAACAAGGCCCCAGAAGCAGTTAAGATAGCGGAGGAAGAGTATAAATCCGTGCAGGCTACAAGTCGGAAGGAGAAGGTTCATCCAGACAAGCCTAAGGAACCGGCACTGCCTCGGAAACACAAGGAGGAGACGTTACTTGTGAGGCAAGTAACACTTCCCAAGGTAGAGGTTAAACTAGCACCACCAACAATTCACATGGGAGAGGCTAGACCGGTCCAAGTAGTAGCGAAAAAGGAAGAGACTAACATGGGAGAGGCTAGACCGGTACAAGTAGTAGCGAAAAAGGAAAAGACTAAGCCAGCAGTGCCAGCGAAACCCAAGAAGCCCTATAATGTCAAGAGGGTAGCAAGTGCAGGGCTTCAGGCCGAGTCTGTCTTGCAAACAAAAACAGGGCACTTGAAGGAGTACTACAATTTGGGCCAAAAGCTCGGACACGGGCAGTTTGGAACGACTTTTCTGTGTGTGGAGAAAGCGACTGGAAAGGAGTATGCTTGTAAATCCATCGCTAAAAGGAAACTCCTGACTACAGAAGATGTGGAGGATGTGAGGAGAGAAATTCAGATTATGCATCACTTGGCGGGGAATCAAAATGTAATCACAATTAAAGCAACATATGAGGATGCTGTTGCAGTTCATGTTGTCATGGAATTATGTGCAGGGGGTGAGCTTTTCGATAGGATTATCCAGCGAGGGCATTACACCGAAAGAAAGGCAGCTCAACTGACGAGGACTATAGTTGGAGTTGTAGAAGCTTGCCATTCTTTGGGTGTCATGCATCGTGATCTTAAGCCGGAGAACTTTCTTTTTGTCAACGAGCAGGAGGATTCACCGCTTAAGGCTATCGATTTTGGACTATCAATATTCTTTAAGCCAGGGGAGATATTCGGTGATGTGGTTGGAAGCCCCTATTATGTTGCCCCAGAAGTCCTGCGCAAGCACTATGGTCCCGAAGCAGATGTTTGGAGTGCCGGTGTTATCATTTACATCCTCTTAAGTGGGGTGCCTCCGTTTTGGGGTGAAACTGAGCAAGAGATATTTGAAGAGGTGCTGCATGGCGATCTTGACTTCTCATCGGATCCCTGGCCTAGAATTTCTGAAAGTGCCAAAGATCTAGTTAAGAGAATGCTCGTAAGAAACCCAAAAAAGCGGCTAACTGCTCATCAAGTTCTCTGCCACCCATGGGTTCAGGTTGATGGAGTGGCTCCAGACAAGGCTCTTGATTCCGCTGTTTTAAGTCGCTTGAAGCAGTTTTCTGCAATGAACAAAATTAAGAAAATGGCTCTTAGAGTCATTGCAGAGAACCTCTCGGAAGAAGAAATTGCTGGTTTAACAGAAATGTTCAAGATGATAGATACTGACAATAGTGGTCAAATTACTTTTGATGAACTCAGAGATGGACTCAAAAGATTTGGAGCTAATCTAAATGAGTCCGAAATATATGATCTTATGCAAGCTGCAGATATTGATAATAGTGGCACAATTGATTACGGAGAGTTTATAGCAGCAACATTGCATCTAAACAAGATAGAGAGAGAAGACCACTTATTTGCAGCTTTCTCATATTTTGACAAGGACGGCAGTGGCTACATCACTCAAGACGAACTTCAACAAGCCTGCGAGGAGTTTGGCATTGTCGATATTCACTTGGAAGAATTGATCCGAGAAGTAGATCAGGACAATGACGGGCAAATAGACTACAACGAGTTCGTTGCCATGATGAAGAAAGGCAATGCAGATTTTGATAAGAAGGGGGTTCACACCAATGCCCTCAGCGTTGGATTTAGGGAGGCACTCTCTGTTTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031984 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0098827 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

658

Amino Acids

73.43

Weight (kDa)

5.86

Isoelectric Point (pI)

40.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	193 - 451	1.8e-75	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	195 - 445	4.3e-22	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	499 - 558	5e-12	EF-hand domain pair
EF-hand_1	PF00036	500 - 525	7.9e-08	EF hand domain
EF-hand_6	PF13405	500 - 527	1.5e-06	EF-hand domain
EF-hand_8	PF13833	511 - 559	2e-09	EF-hand domain pair
EF-hand_7	PF13499	568 - 630	2.2e-15	EF-hand domain pair
EF-hand_1	PF00036	572 - 597	4e-06	EF hand domain
EF-hand_6	PF13405	572 - 599	6.4e-07	EF-hand domain
EF-hand_1	PF00036	605 - 631	1.4e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10660 AT3G10660 AT5G04870
fragaria_vesca	FvH4_6g20840 FvH4_6g20840 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490
malus_domestica	MD02G1257300.v1.1 MD07G1063300.v1.1 MD12G1044400.v1.1 MD14G1043300.v1.1
prunus_persica	Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.7G090800_v2.0.a1 Prupe.7G090800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0332341 RchiOBHm_Chr3g0464211 RchiOBHm_Chr3g0477181
rosa_laevigata	RLG00000023739 RLG00000029678
rosa_multiflora	Rmu_sc0000932.1_g000001 Rmu_sc0003422.1_g000006
rosa_roxburghii	Rroxscaffold_4G00318280 Rroxscaffold_6G00404350 Rroxscaffold_6G00404850
rosa_rugosa	Rorug01G0100000 Rorug03G0158600
rosa_samantha	Rh1CG118000 Rh1DG129100 Rh3AG208900 Rh3CG236600 Rh3DG235400
rosa_wichuraiana	Rw1G010080 Rw3G019050 Rw3G019440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1100
AccB1I	GGYRCC	1 cut(s)	1168
AccB7I	CCANNNNNTGG	1 cut(s)	931
AciI	CCGC	7 cut(s)	84, 125, 197, 746, 998, 1315, 1392
AclWI	GGATC	4 cut(s)	28, 1238, 1251, 1819
AcsI	RAATTY	3 cut(s)	55, 723, 1257
AcuI	CTGAAG	1 cut(s)	512
AfaI	GTAC	3 cut(s)	112, 435, 575
AfiI	CCNNNNNNNGG	3 cut(s)	931, 1181, 1348
AflII	CTTAAG	3 cut(s)	953, 1001, 1159
AgeI	ACCGGT	2 cut(s)	379, 430
AgsI	TTSAA	6 cut(s)	167, 568, 1208, 1411, 1510, 1775
AjnI	CCWGG	2 cut(s)	1039, 1247
AleI	CACNNNNGTG	1 cut(s)	64
AluBI	AGCT	8 cut(s)	152, 595, 835, 884, 920, 1583, 1622, 1721
AluI	AGCT	8 cut(s)	152, 595, 835, 884, 920, 1583, 1622, 1721
Alw26I	GTCTC	4 cut(s)	55, 75, 290, 401
AlwI	GGATC	4 cut(s)	28, 1238, 1251, 1819
AoxI	GGCC	4 cut(s)	174, 531, 586, 1250
ApeKI	GCWGC	5 cut(s)	881, 1216, 1622, 1670, 1718
ApoI	RAATTY	3 cut(s)	55, 723, 1257
ArsI	GACNNNNNNTTYG	2 cut(s)	1697, 1729
AsiGI	ACCGGT	2 cut(s)	379, 430
AspLEI	GCGC	1 cut(s)	1101
AspS9I	GGNCC	4 cut(s)	175, 382, 586, 1112
AsuHPI	GGTGA	4 cut(s)	842, 987, 1067, 1196
AvaII	GGWCC	2 cut(s)	382, 1112
AxyI	CCTNAGG	1 cut(s)	261
BaeGI	GKGCMC	1 cut(s)	564
BamHI	GGATCC	1 cut(s)	1243
BanI	GGYRCC	1 cut(s)	1168
BarI	GAAGNNNNNNTAC	2 cut(s)	560, 592
BbsI	GAAGAC	1 cut(s)	1710
BbvCI	CCTCAGC	1 cut(s)	1937
BbvI	GCAGC	5 cut(s)	893, 1203, 1609, 1682, 1730
BccI	CCATC	3 cut(s)	677, 1355, 1556
BceAI	ACGGC	1 cut(s)	1759
BcgI	CGANNNNNNTGC	2 cut(s)	1859, 1893
BciT130I	CCWGG	2 cut(s)	1041, 1249
BcoDI	GTCTC	4 cut(s)	55, 75, 290, 401
BfaI	CTAG	5 cut(s)	344, 375, 426, 1254, 1280
BfmI	CTRYAG	3 cut(s)	693, 900, 1623
BfrI	CTTAAG	3 cut(s)	953, 1001, 1159
BglII	AGATCT	1 cut(s)	1276
BisI	GCNGC	7 cut(s)	125, 882, 1217, 1316, 1623, 1671, 1719
BlsI	GCNGC	7 cut(s)	126, 883, 1218, 1317, 1624, 1672, 1720
BmcAI	AGTACT	2 cut(s)	112, 575
Bme1390I	CCNGG	2 cut(s)	1041, 1249
Bme18I	GGWCC	2 cut(s)	382, 1112
BmgT120I	GGNCC	4 cut(s)	175, 382, 586, 1112
BmiI	GGNNCC	6 cut(s)	177, 267, 1114, 1170, 1245, 1371
BmrFI	CCNGG	2 cut(s)	1041, 1249
BmsI	GCATC	4 cut(s)	745, 778, 952, 1690
BpiI	GAAGAC	1 cut(s)	1710
BplI	GAGNNNNNCTC	5 cut(s)	1457, 1489, 1551, 1583, 1946
BpmI	CTGGAG	1 cut(s)	1356
Bpu10I	CCTNAGC	1 cut(s)	1937
BpuEI	CTTGAG	1 cut(s)	1746
Bsa29I	ATCGAT	1 cut(s)	1011
BsaI	GGTCTC	1 cut(s)	75
BsaJI	CCNNGG	5 cut(s)	278, 327, 1040, 1247, 1346
BsaWI	WCCGGW	2 cut(s)	379, 430
BsaXI	ACNNNNNCTCC	6 cut(s)	191, 221, 284, 314, 1123, 1153
Bsc4I	CCNNNNNNNGG	3 cut(s)	931, 1181, 1348
Bse118I	RCCGGY	4 cut(s)	268, 379, 430, 1136
Bse1I	ACTGG	2 cut(s)	93, 649
Bse21I	CCTNAGG	1 cut(s)	261
Bse3DI	GCAATG	4 cut(s)	1431, 1458, 1676, 1903
BseBI	CCWGG	2 cut(s)	1041, 1249
BseCI	ATCGAT	1 cut(s)	1011
BseDI	CCNNGG	5 cut(s)	278, 327, 1040, 1247, 1346
BseGI	GGATG	5 cut(s)	39, 247, 715, 793, 1152
BseLI	CCNNNNNNNGG	3 cut(s)	931, 1181, 1348
BseMI	GCAATG	4 cut(s)	1431, 1458, 1676, 1903
BseMII	CTCAG	3 cut(s)	1182, 1570, 1951
BseNI	ACTGG	2 cut(s)	93, 649
BseRI	GAGGAG	3 cut(s)	308, 730, 1802
BseSI	GKGCMC	1 cut(s)	564
BseXI	GCAGC	5 cut(s)	893, 1203, 1609, 1682, 1730
BsgI	GTGCAG	3 cut(s)	239, 540, 843
BshFI	GGCC	4 cut(s)	176, 533, 588, 1252
BshNI	GGYRCC	1 cut(s)	1168
BshTI	ACCGGT	2 cut(s)	379, 430
BshVI	ATCGAT	1 cut(s)	1011
BsiSI	CCGG	5 cut(s)	269, 380, 431, 959, 1137
BslFI	GGGAC	1 cut(s)	1098
BslI	CCNNNNNNNGG	3 cut(s)	931, 1181, 1348
BsmAI	GTCTC	4 cut(s)	55, 75, 290, 401
BsmBI	CGTCTC	1 cut(s)	290
BsmFI	GGGAC	1 cut(s)	1098
BsmI	GAATGC	1 cut(s)	1296
BsnI	GGCC	4 cut(s)	176, 533, 588, 1252
Bso31I	GGTCTC	1 cut(s)	75
Bsp1286I	GDGCHC	1 cut(s)	564
Bsp143I	GATC	9 cut(s)	20, 74, 949, 1225, 1243, 1276, 1609, 1824, 1837
Bsp19I	CCATGG	1 cut(s)	1346
BspACI	CCGC	7 cut(s)	84, 125, 197, 746, 998, 1315, 1392
BspANI	GGCC	4 cut(s)	176, 533, 588, 1252
BspCNI	CTCAG	3 cut(s)	1183, 1569, 1950
BspDI	ATCGAT	1 cut(s)	1011
BspLI	GGNNCC	6 cut(s)	177, 267, 1114, 1170, 1245, 1371
BspMAI	CTGCAG	1 cut(s)	1627
BspPI	GGATC	4 cut(s)	28, 1238, 1251, 1819
BspT107I	GGYRCC	1 cut(s)	1168
BspTI	CTTAAG	3 cut(s)	953, 1001, 1159
BspTNI	GGTCTC	1 cut(s)	75
BsrDI	GCAATG	4 cut(s)	1431, 1458, 1676, 1903
BsrFI	RCCGGY	4 cut(s)	268, 379, 430, 1136
BsrI	ACTGG	2 cut(s)	93, 649
BssAI	RCCGGY	4 cut(s)	268, 379, 430, 1136
BssECI	CCNNGG	5 cut(s)	278, 327, 1040, 1247, 1346
BssMI	GATC	9 cut(s)	20, 74, 949, 1225, 1243, 1276, 1609, 1824, 1837
BssT1I	CCWWGG	2 cut(s)	327, 1346
Bst2UI	CCWGG	2 cut(s)	1041, 1249
Bst6I	CTCTTC	3 cut(s)	198, 399, 1203
BstAFI	CTTAAG	3 cut(s)	953, 1001, 1159
BstC8I	GCNNGC	7 cut(s)	222, 468, 477, 922, 1103, 1620, 1783
BstDEI	CTNAG	6 cut(s)	261, 462, 1191, 1451, 1556, 1937
BstDSI	CCRYGG	1 cut(s)	1346
BstF5I	GGATG	5 cut(s)	39, 247, 715, 793, 1152
BstHHI	GCGC	1 cut(s)	1101
BstKTI	GATC	9 cut(s)	23, 77, 952, 1228, 1246, 1279, 1612, 1827, 1840
BstMAI	GTCTC	4 cut(s)	55, 75, 290, 401
BstMBI	GATC	9 cut(s)	20, 74, 949, 1225, 1243, 1276, 1609, 1824, 1837
BstMWI	GCNNNNNNNGC	3 cut(s)	182, 472, 476
BstNI	CCWGG	2 cut(s)	1041, 1249
BstSCI	CCNGG	2 cut(s)	1039, 1247
BstSFI	CTRYAG	3 cut(s)	693, 900, 1623
BstSLI	GKGCMC	1 cut(s)	564
BstV1I	GCAGC	5 cut(s)	893, 1203, 1609, 1682, 1730
BstV2I	GAAGAC	1 cut(s)	1710
BstX2I	RGATCY	2 cut(s)	1243, 1276
BstYI	RGATCY	2 cut(s)	1243, 1276
Bsu15I	ATCGAT	1 cut(s)	1011
Bsu36I	CCTNAGG	1 cut(s)	261
BsuRI	GGCC	4 cut(s)	176, 533, 588, 1252
BsuTUI	ATCGAT	1 cut(s)	1011
BtgI	CCRYGG	1 cut(s)	1346
BtgZI	GCGATG	1 cut(s)	655
BtsCI	GGATG	5 cut(s)	39, 247, 715, 793, 1152
BtsI	GCAGTG	3 cut(s)	272, 477, 1753
BtsIMutI	CAGTG	3 cut(s)	272, 477, 1753
Cac8I	GCNNGC	7 cut(s)	222, 468, 477, 922, 1103, 1620, 1783
CfoI	GCGC	1 cut(s)	1101
Cfr10I	RCCGGY	4 cut(s)	268, 379, 430, 1136
Cfr13I	GGNCC	4 cut(s)	175, 382, 586, 1112
ClaI	ATCGAT	1 cut(s)	1011
CseI	GACGC	1 cut(s)	4
Csp6I	GTAC	3 cut(s)	111, 434, 574
CspAI	ACCGGT	2 cut(s)	379, 430
CviAII	CATG	8 cut(s)	364, 415, 803, 811, 940, 1220, 1347, 1882
CviQI	GTAC	3 cut(s)	111, 434, 574
DdeI	CTNAG	6 cut(s)	261, 462, 1191, 1451, 1556, 1937
DpnI	GATC	9 cut(s)	22, 76, 951, 1227, 1245, 1278, 1611, 1826, 1839
DpnII	GATC	9 cut(s)	20, 74, 949, 1225, 1243, 1276, 1609, 1824, 1837
Eam1104I	CTCTTC	3 cut(s)	198, 399, 1203
EarI	CTCTTC	3 cut(s)	198, 399, 1203
Eco130I	CCWWGG	2 cut(s)	327, 1346
Eco31I	GGTCTC	1 cut(s)	75
Eco47I	GGWCC	2 cut(s)	382, 1112
Eco57I	CTGAAG	1 cut(s)	512
Eco81I	CCTNAGG	1 cut(s)	261
EcoO109I	RGGNCCY	1 cut(s)	175
EcoRI	GAATTC	1 cut(s)	55
EcoRII	CCWGG	2 cut(s)	1039, 1247
EcoT14I	CCWWGG	2 cut(s)	327, 1346
EcoT22I	ATGCAT	2 cut(s)	738, 945
ErhI	CCWWGG	2 cut(s)	327, 1346
Esp3I	CGTCTC	1 cut(s)	290
FaeI	CATG	8 cut(s)	367, 418, 806, 814, 943, 1223, 1350, 1885
FalI	AAGNNNNNCTT	6 cut(s)	141, 173, 306, 338, 1755, 1787
FaqI	GGGAC	1 cut(s)	1098
FatI	CATG	8 cut(s)	363, 414, 802, 810, 939, 1219, 1346, 1881
FauI	CCCGC	1 cut(s)	739
FauNDI	CATATG	1 cut(s)	781
Fnu4HI	GCNGC	7 cut(s)	125, 882, 1217, 1316, 1623, 1671, 1719
FokI	GGATG	5 cut(s)	26, 234, 722, 800, 1139
Fsp4HI	GCNGC	7 cut(s)	125, 882, 1217, 1316, 1623, 1671, 1719
FspBI	CTAG	5 cut(s)	344, 375, 426, 1254, 1280
FspI	TGCGCA	1 cut(s)	1100
GlaI	GCGC	1 cut(s)	1100
GluI	GCNGC	7 cut(s)	125, 882, 1217, 1316, 1623, 1671, 1719
GsuI	CTGGAG	1 cut(s)	1356
HaeIII	GGCC	4 cut(s)	176, 533, 588, 1252
HapII	CCGG	5 cut(s)	269, 380, 431, 959, 1137
HgaI	GACGC	1 cut(s)	4
HhaI	GCGC	1 cut(s)	1101
Hin1II	CATG	8 cut(s)	367, 418, 806, 814, 943, 1223, 1350, 1885
Hin6I	GCGC	1 cut(s)	1099
HinP1I	GCGC	1 cut(s)	1099
HincII	GTYRAC	1 cut(s)	979
HindII	GTYRAC	1 cut(s)	979
HindIII	AAGCTT	1 cut(s)	918
HinfI	GANTC	9 cut(s)	102, 139, 536, 751, 992, 1388, 1455, 1566, 1595
HpaII	CCGG	5 cut(s)	269, 380, 431, 959, 1137
HphI	GGTGA	4 cut(s)	842, 987, 1067, 1196
Hpy166II	GTNNAC	2 cut(s)	979, 1924
Hpy8I	GTNNAC	2 cut(s)	979, 1924
Hpy99I	CGWCG	1 cut(s)	20
HpyAV	CCTTC	4 cut(s)	229, 235, 562, 1909
HpyCH4IV	ACGT	1 cut(s)	299
HpyF10VI	GCNNNNNNNGC	3 cut(s)	182, 472, 476
HpyF3I	CTNAG	6 cut(s)	261, 462, 1191, 1451, 1556, 1937
HpySE526I	ACGT	1 cut(s)	299
Hsp92II	CATG	8 cut(s)	367, 418, 806, 814, 943, 1223, 1350, 1885
HspAI	GCGC	1 cut(s)	1099
Kzo9I	GATC	9 cut(s)	20, 74, 949, 1225, 1243, 1276, 1609, 1824, 1837
LmnI	GCTCC	2 cut(s)	1375, 1580
Lsp1109I	GCAGC	5 cut(s)	893, 1203, 1609, 1682, 1730
LweI	GCATC	4 cut(s)	745, 778, 952, 1690
MaeI	CTAG	5 cut(s)	344, 375, 426, 1254, 1280
MaeII	ACGT	1 cut(s)	299
MaeIII	GTNAC	2 cut(s)	300, 316
MalI	GATC	9 cut(s)	22, 76, 951, 1227, 1245, 1278, 1611, 1826, 1839
MboI	GATC	9 cut(s)	20, 74, 949, 1225, 1243, 1276, 1609, 1824, 1837
MfeI	CAATTG	2 cut(s)	128, 1647
MflI	RGATCY	2 cut(s)	1243, 1276
MhlI	GDGCHC	1 cut(s)	564
MlyI	GAGTC	4 cut(s)	545, 1464, 1560, 1604
MmeI	TCCRAC	4 cut(s)	212, 886, 1045, 1924
Mph1103I	ATGCAT	2 cut(s)	738, 945
MslI	CAYNNNNRTG	2 cut(s)	64, 1929
MspA1I	CMGCKG	1 cut(s)	1394
MspCI	CTTAAG	3 cut(s)	953, 1001, 1159
MspI	CCGG	5 cut(s)	269, 380, 431, 959, 1137
MspR9I	CCNGG	2 cut(s)	1041, 1249
MunI	CAATTG	2 cut(s)	128, 1647
Mva1269I	GAATGC	1 cut(s)	1296
MvaI	CCWGG	2 cut(s)	1041, 1249
MwoI	GCNNNNNNNGC	3 cut(s)	182, 472, 476
NcoI	CCATGG	1 cut(s)	1346
NdeI	CATATG	1 cut(s)	781
NdeII	GATC	9 cut(s)	20, 74, 949, 1225, 1243, 1276, 1609, 1824, 1837
NlaIII	CATG	8 cut(s)	367, 418, 806, 814, 943, 1223, 1350, 1885
NlaIV	GGNNCC	6 cut(s)	177, 267, 1114, 1170, 1245, 1371
NmeAIII	GCCGAG	1 cut(s)	559
NsbI	TGCGCA	1 cut(s)	1100
NsiI	ATGCAT	2 cut(s)	738, 945
OliI	CACNNNNGTG	1 cut(s)	64
PctI	GAATGC	1 cut(s)	1296
PfeI	GAWTC	5 cut(s)	102, 139, 751, 992, 1388
PflMI	CCANNNNNTGG	1 cut(s)	931
PinAI	ACCGGT	2 cut(s)	379, 430
PkrI	GCNGC	7 cut(s)	126, 883, 1218, 1317, 1624, 1672, 1720
PleI	GAGTC	4 cut(s)	544, 1463, 1560, 1603
PpsI	GAGTC	4 cut(s)	544, 1463, 1560, 1603
Psp6I	CCWGG	2 cut(s)	1039, 1247
PspGI	CCWGG	2 cut(s)	1039, 1247
PspN4I	GGNNCC	6 cut(s)	177, 267, 1114, 1170, 1245, 1371
PspPI	GGNCC	4 cut(s)	175, 382, 586, 1112
PstI	CTGCAG	1 cut(s)	1627
PsuI	RGATCY	2 cut(s)	1243, 1276
RsaI	GTAC	3 cut(s)	112, 435, 575
RsaNI	GTAC	3 cut(s)	111, 434, 574
RseI	CAYNNNNRTG	2 cut(s)	64, 1929
SatI	GCNGC	7 cut(s)	125, 882, 1217, 1316, 1623, 1671, 1719
Sau3AI	GATC	9 cut(s)	20, 74, 949, 1225, 1243, 1276, 1609, 1824, 1837
Sau96I	GGNCC	4 cut(s)	175, 382, 586, 1112
ScaI	AGTACT	2 cut(s)	112, 575
SchI	GAGTC	4 cut(s)	545, 1464, 1560, 1604
ScrFI	CCNGG	2 cut(s)	1041, 1249
SduI	GDGCHC	1 cut(s)	564
SfaNI	GCATC	4 cut(s)	745, 778, 952, 1690
SfcI	CTRYAG	3 cut(s)	693, 900, 1623
SinI	GGWCC	2 cut(s)	382, 1112
SmiMI	CAYNNNNRTG	2 cut(s)	64, 1929
SmlI	CTYRAG	4 cut(s)	953, 1001, 1159, 1761
SmoI	CTYRAG	4 cut(s)	953, 1001, 1159, 1761
SsiI	CCGC	7 cut(s)	84, 125, 197, 746, 998, 1315, 1392
SspI	AATATT	1 cut(s)	1029
SspMI	CTAG	5 cut(s)	344, 375, 426, 1254, 1280
StyD4I	CCNGG	2 cut(s)	1039, 1247
StyI	CCWWGG	2 cut(s)	327, 1346
TaiI	ACGT	1 cut(s)	302
TaqI	TCGA	5 cut(s)	53, 73, 840, 1011, 1803
TatI	WGTACW	2 cut(s)	110, 573
TauI	GCSGC	2 cut(s)	127, 1318
TfiI	GAWTC	5 cut(s)	102, 139, 751, 992, 1388
TscAI	CASTG	3 cut(s)	279, 477, 1753
TseI	GCWGC	5 cut(s)	881, 1216, 1622, 1670, 1718
TspDTI	ATGAA	6 cut(s)	132, 236, 791, 1442, 1566, 1901
TspGWI	ACGGA	4 cut(s)	132, 205, 1164, 1671
TspRI	CASTG	3 cut(s)	279, 477, 1753
Van91I	CCANNNNNTGG	1 cut(s)	931
Vha464I	CTTAAG	3 cut(s)	953, 1001, 1159
VpaK11BI	GGWCC	2 cut(s)	382, 1112
XapI	RAATTY	3 cut(s)	55, 723, 1257
XspI	CTAG	5 cut(s)	344, 375, 426, 1254, 1280
ZrmI	AGTACT	2 cut(s)	112, 575
Zsp2I	ATGCAT	2 cut(s)	738, 945

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.