Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0002410

Physical Location & Seq

Genomic Coordinates Forward (+)

22483377 .. 22487562

Gene Structure 4186 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG236600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1914 bp

ATGGGAAACAACTGTGTTGGAAATTCCAAGGACGGGCTTTTCCATTCGGTCTCAAATTCACTCTGGTGGACTCGATCAACCGGGGGTTTGGTCACTTCTACAACTAGAGAAATTAAAAGTAGTGATGCACCACCATCATTGAACAATCAGTCAGAAACTAATCCTCATGATGTTCAAAGCAATCCCCCAGAAGCAATCAAGATGACTACGGAGGGCTCTAAACCAGTGCAGCCCCCAATTCAACCGACGAAGGTTCATCCCGAAAAGCAAAAGGAAGAGGAGACTGAACCAGCAGCACAAGCAGCAATTCCGAAGGGAGAGACTAAACCTCAACAACCGGTAAATCATAGGGATCAAGAGACCAGACCAGCGCAATCCATAACACAAAATGGAGGGACTAATCAGCCAGCAGTGCCTGCGAGACCGAAGAAGCCCCACCATGTCAAGAGGGTATCGAGTGCAGGGCTTCAGGCAGAGTCTGTCTTGCAAACCAAGACAGGTCATTTGAAGGAGTACTACAATTTGGGCCAGAAGCTTGGACATGGACAATTTGGGACAACTTACCTTTGTGTGGAGAAAGCAACCGGAAAGGAATACGCATGCAAATCCATTGCCAAAAGGAAATTGTTGGCAACAGAAGATGTGGAGGATGTGAGGAGGGAGATTCAAATAATGCATCACTTGGCAGGGAATCCCAATGTCATATCGATTAAGGCGGCTTATGAGGATGCTGTTGCAGTTCATGTTGTGATGGAATTATGTGCAGGGGGTGAGCTCTTTGATAGGATTATCAAGAGGGGGCATTACTCTGAAAGAAAGGCAGCTCGGCTAGCAAGGACTATAGTTGGAGTTATAGAAGCTTGCCATTCTTTGGGTGTAATGCATCGCGATCTTAAACCCGAGAACTTTCTTTTTGTTGATGAGGAGGAGGATTCAGCACTCAAAGCTATAGATTTTGGGCTGTCAATATTTTTCAAGCCAGGTGAAACTTTCAATGATGTGGTTGGAAGCCCATATTATGTTGCACCTGAAGTTCTGCGCAAGCGGTATGGTCCAGAAGCAGATGTTTGGAGTGTTGGTGTTATCATTTACATTCTTTTGAGTGGGGTGCCTCCATTCTGGGGTGAAACTGAGCAAGAGATCTTTGAAGAGGTTTTGCATGGTGATCTTGACTTCTCAACAGATCCCTGGCCTAATATTTCAGAAAGTGCGAAAGATTTAGTCAAGAAGATGCTTGTCAGAAACCCCAAAAAGCGGCTAACTGCTCATCAAGTTCTATGTCACCCTTGGGTTCAGGTTGATGGAGTGGCTCCAGACAAGCCACTTGATTCTGCAGTCTTAAGTCGCTTGAAGCAGTTCTCTGCAATGAACAAAATTAAGAAAATGGCTCTTAGAGTCATTGCTGAAAACCTCTCTGAAGAAGAAATTGCCGGTTTAAAAGAAATGTTCAAGATGATAGATACTGACAATAGTGGTCAAATTAGTTTCGAAGAACTGAAAGATGGACTGAAAAGATTTGGAGCTACTCTAAATGAGTCTGAAATATACGACCTGATGCAAGCTGCTGATGTTGACAACAGTGGCACAATTGATTATGGGGAGTTCATAGCAGCAACATTGCATTTAAACAAAGTAGAGAGGGAAGATCATTTGTTTGCAGCTTTCTCATATTTTGACAAAGATGGCAGTGGCTATATAACTCAAGATGAACTTCAACAAGCGTGTGAGGAGTTTGGCATTCAGGATGTCCACTTGGAAGAGTTGATCCGAGAAGTCGATCAGGACAATGATGGTCGAATAGATTACAATGAGTTCGTTGCCATGATGCAGAAAGGCAATCCAGAGTTTGATAAGAAGGGTATTCAAACCAGTGCTCTTGGCATTGGATTTAGGGAGGCACTATCTGTTTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031984 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0098827 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

637

Amino Acids

70.85

Weight (kDa)

5.41

Isoelectric Point (pI)

44.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	173 - 431	1.8e-77	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	175 - 425	2e-24	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	479 - 505	1.1e-08	EF hand domain
EF-hand_7	PF13499	479 - 538	8.4e-13	EF-hand domain pair
EF-hand_6	PF13405	479 - 507	4.3e-07	EF-hand domain
EF-hand_5	PF13202	479 - 501	2.7e-06	EF hand
EF-hand_8	PF13833	493 - 539	6e-07	EF-hand domain pair
EF-hand_7	PF13499	548 - 610	2.2e-16	EF-hand domain pair
EF-hand_1	PF00036	552 - 577	3.8e-06	EF hand domain
EF-hand_6	PF13405	552 - 579	6.1e-07	EF-hand domain
EF-hand_1	PF00036	585 - 611	4.5e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10660 AT3G10660 AT5G04870
fragaria_vesca	FvH4_6g20840 FvH4_6g20840 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490
malus_domestica	MD02G1257300.v1.1 MD07G1063300.v1.1 MD12G1044400.v1.1 MD14G1043300.v1.1
prunus_persica	Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.7G090800_v2.0.a1 Prupe.7G090800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0332341 RchiOBHm_Chr3g0464211 RchiOBHm_Chr3g0477181
rosa_laevigata	RLG00000023739 RLG00000029678
rosa_multiflora	Rmu_sc0000932.1_g000001 Rmu_sc0003422.1_g000006
rosa_roxburghii	Rroxscaffold_4G00318280 Rroxscaffold_6G00404350 Rroxscaffold_6G00404850
rosa_rugosa	Rorug01G0100000 Rorug03G0158600
rosa_samantha	Rh1CG118000 Rh1DG129100 Rh3AG208900 Rh3CG236600 Rh3DG235400
rosa_wichuraiana	Rw1G010080 Rw3G019050 Rw3G019440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1040
AccB1I	GGYRCC	1 cut(s)	1108
AccB7I	CCANNNNNTGG	1 cut(s)	871
AccII	CGCG	1 cut(s)	888
AciI	CCGC	3 cut(s)	716, 1045, 1255
AclWI	GGATC	3 cut(s)	360, 1178, 1759
AcsI	RAATTY	2 cut(s)	22, 55
AcuI	CTGAAG	3 cut(s)	452, 1050, 1437
AfaI	GTAC	1 cut(s)	515
AfiI	CCNNNNNNNGG	5 cut(s)	33, 571, 871, 1121, 1254
AflII	CTTAAG	1 cut(s)	1339
AgeI	ACCGGT	1 cut(s)	337
AjnI	CCWGG	2 cut(s)	979, 1187
AleI	CACNNNNGTG	1 cut(s)	64
AluBI	AGCT	8 cut(s)	535, 775, 824, 860, 947, 1523, 1562, 1661
AluI	AGCT	8 cut(s)	535, 775, 824, 860, 947, 1523, 1562, 1661
Alw21I	GWGCWC	2 cut(s)	777, 1876
Alw26I	GTCTC	5 cut(s)	55, 275, 314, 353, 415
AlwI	GGATC	3 cut(s)	360, 1178, 1759
AlwNI	CAGNNNCTG	2 cut(s)	416, 479
Ama87I	CYCGRG	1 cut(s)	899
AoxI	GGCC	2 cut(s)	526, 1190
ApeKI	GCWGC	7 cut(s)	229, 293, 302, 821, 1562, 1610, 1658
ApoI	RAATTY	2 cut(s)	22, 55
ArsI	GACNNNNNNTTYG	2 cut(s)	487, 519
AsiGI	ACCGGT	1 cut(s)	337
Asp700I	GAANNNNTTC	1 cut(s)	1355
AspLEI	GCGC	2 cut(s)	373, 1041
AspS9I	GGNCC	2 cut(s)	526, 1052
AsuC2I	CCSGG	1 cut(s)	82
AsuHPI	GGTGA	5 cut(s)	782, 995, 1136, 1175, 1274
AsuII	TTCGAA	1 cut(s)	1488
AsuNHI	GCTAGC	1 cut(s)	829
AvaI	CYCGRG	1 cut(s)	899
AvaII	GGWCC	1 cut(s)	1052
BanI	GGYRCC	1 cut(s)	1108
BanII	GRGCYC	2 cut(s)	218, 777
BarI	GAAGNNNNNNTAC	2 cut(s)	500, 532
Bbv12I	GWGCWC	2 cut(s)	777, 1876
BbvI	GCAGC	7 cut(s)	241, 305, 314, 833, 1549, 1622, 1670
BccI	CCATC	6 cut(s)	142, 745, 1295, 1496, 1676, 1784
BciT130I	CCWGG	2 cut(s)	981, 1189
BcnI	CCSGG	1 cut(s)	82
BcoDI	GTCTC	5 cut(s)	55, 275, 314, 353, 415
BfaI	CTAG	2 cut(s)	105, 830
BfmI	CTRYAG	3 cut(s)	840, 948, 1332
BfrI	CTTAAG	1 cut(s)	1339
BglII	AGATCT	1 cut(s)	1140
BisI	GCNGC	9 cut(s)	230, 294, 303, 717, 822, 1256, 1563, 1611, 1659
BlsI	GCNGC	9 cut(s)	231, 295, 304, 718, 823, 1257, 1564, 1612, 1660
BmcAI	AGTACT	1 cut(s)	515
Bme1390I	CCNGG	3 cut(s)	82, 981, 1189
Bme18I	GGWCC	1 cut(s)	1052
BmeT110I	CYCGRG	1 cut(s)	899
BmgT120I	GGNCC	2 cut(s)	526, 1052
BmiI	GGNNCC	2 cut(s)	1110, 1311
BmrFI	CCNGG	3 cut(s)	82, 981, 1189
BmsI	GCATC	7 cut(s)	115, 685, 718, 892, 1221, 1545, 1815
BmtI	GCTAGC	1 cut(s)	833
BpmI	CTGGAG	1 cut(s)	1296
Bpu14I	TTCGAA	1 cut(s)	1488
BpuEI	CTTGAG	1 cut(s)	1686
BpuMI	CCSGG	1 cut(s)	82
Bsa29I	ATCGAT	1 cut(s)	707
BsaI	GGTCTC	3 cut(s)	55, 353, 415
BsaJI	CCNNGG	4 cut(s)	27, 81, 1187, 1286
BsaWI	WCCGGW	2 cut(s)	337, 584
BsaXI	ACNNNNNCTCC	2 cut(s)	1063, 1093
Bsc4I	CCNNNNNNNGG	5 cut(s)	33, 571, 871, 1121, 1254
Bse118I	RCCGGY	2 cut(s)	337, 1430
Bse1I	ACTGG	2 cut(s)	224, 1869
Bse3DI	GCAATG	4 cut(s)	609, 1371, 1398, 1616
BseBI	CCWGG	2 cut(s)	981, 1189
BseCI	ATCGAT	1 cut(s)	707
BseDI	CCNNGG	4 cut(s)	27, 81, 1187, 1286
BseGI	GGATG	4 cut(s)	256, 655, 733, 1750
BseLI	CCNNNNNNNGG	5 cut(s)	33, 571, 871, 1121, 1254
BseMI	GCAATG	4 cut(s)	609, 1371, 1398, 1616
BseMII	CTCAG	1 cut(s)	1122
BseNI	ACTGG	2 cut(s)	224, 1869
BseRI	GAGGAG	5 cut(s)	293, 670, 938, 941, 1742
BseXI	GCAGC	7 cut(s)	241, 305, 314, 833, 1549, 1622, 1670
BsgI	GTGCAG	3 cut(s)	248, 480, 783
Bsh1236I	CGCG	1 cut(s)	888
BshFI	GGCC	2 cut(s)	528, 1192
BshNI	GGYRCC	1 cut(s)	1108
BshTI	ACCGGT	1 cut(s)	337
BshVI	ATCGAT	1 cut(s)	707
BsiHKAI	GWGCWC	2 cut(s)	777, 1876
BsiHKCI	CYCGRG	1 cut(s)	899
BsiSI	CCGG	4 cut(s)	81, 338, 585, 1431
BslFI	GGGAC	2 cut(s)	409, 568
BslI	CCNNNNNNNGG	5 cut(s)	33, 571, 871, 1121, 1254
BsmAI	GTCTC	5 cut(s)	55, 275, 314, 353, 415
BsmFI	GGGAC	2 cut(s)	409, 568
BsmI	GAATGC	1 cut(s)	1737
BsnI	GGCC	2 cut(s)	528, 1192
Bso31I	GGTCTC	3 cut(s)	55, 353, 415
BsoBI	CYCGRG	1 cut(s)	899
Bsp119I	TTCGAA	1 cut(s)	1488
Bsp1286I	GDGCHC	3 cut(s)	218, 777, 1876
Bsp143I	GATC	9 cut(s)	74, 352, 889, 1140, 1165, 1183, 1645, 1764, 1777
Bsp68I	TCGCGA	1 cut(s)	888
BspACI	CCGC	3 cut(s)	716, 1045, 1255
BspANI	GGCC	2 cut(s)	528, 1192
BspCNI	CTCAG	1 cut(s)	1123
BspDI	ATCGAT	1 cut(s)	707
BspFNI	CGCG	1 cut(s)	888
BspHI	TCATGA	1 cut(s)	166
BspLI	GGNNCC	2 cut(s)	1110, 1311
BspMAI	CTGCAG	1 cut(s)	1336
BspOI	GCTAGC	1 cut(s)	833
BspPI	GGATC	3 cut(s)	360, 1178, 1759
BspT104I	TTCGAA	1 cut(s)	1488
BspT107I	GGYRCC	1 cut(s)	1108
BspTI	CTTAAG	1 cut(s)	1339
BspTNI	GGTCTC	3 cut(s)	55, 353, 415
BsrDI	GCAATG	4 cut(s)	609, 1371, 1398, 1616
BsrFI	RCCGGY	2 cut(s)	337, 1430
BsrI	ACTGG	2 cut(s)	224, 1869
BssAI	RCCGGY	2 cut(s)	337, 1430
BssECI	CCNNGG	4 cut(s)	27, 81, 1187, 1286
BssMI	GATC	9 cut(s)	74, 352, 889, 1140, 1165, 1183, 1645, 1764, 1777
BssT1I	CCWWGG	2 cut(s)	27, 1286
Bst2UI	CCWGG	2 cut(s)	981, 1189
Bst4CI	ACNGT	2 cut(s)	14, 1580
Bst6I	CTCTTC	3 cut(s)	270, 1143, 1752
BstAFI	CTTAAG	1 cut(s)	1339
BstAPI	GCANNNNNTGC	1 cut(s)	416
BstBI	TTCGAA	1 cut(s)	1488
BstC8I	GCNNGC	7 cut(s)	408, 417, 601, 831, 862, 1043, 1560
BstDEI	CTNAG	2 cut(s)	1131, 1391
BstF5I	GGATG	4 cut(s)	256, 655, 733, 1750
BstFNI	CGCG	1 cut(s)	888
BstHHI	GCGC	2 cut(s)	373, 1041
BstKTI	GATC	9 cut(s)	77, 355, 892, 1143, 1168, 1186, 1648, 1767, 1780
BstMAI	GTCTC	5 cut(s)	55, 275, 314, 353, 415
BstMBI	GATC	9 cut(s)	74, 352, 889, 1140, 1165, 1183, 1645, 1764, 1777
BstMWI	GCNNNNNNNGC	6 cut(s)	299, 302, 412, 416, 830, 944
BstNI	CCWGG	2 cut(s)	981, 1189
BstNSI	RCATGY	1 cut(s)	603
BstSCI	CCNGG	3 cut(s)	80, 979, 1187
BstSFI	CTRYAG	3 cut(s)	840, 948, 1332
BstUI	CGCG	1 cut(s)	888
BstV1I	GCAGC	7 cut(s)	241, 305, 314, 833, 1549, 1622, 1670
BstX2I	RGATCY	2 cut(s)	1140, 1183
BstXI	CCANNNNNNTGG	1 cut(s)	536
BstYI	RGATCY	2 cut(s)	1140, 1183
Bsu15I	ATCGAT	1 cut(s)	707
BsuRI	GGCC	2 cut(s)	528, 1192
BsuTUI	ATCGAT	1 cut(s)	707
BtgZI	GCGATG	1 cut(s)	869
BtsCI	GGATG	4 cut(s)	256, 655, 733, 1750
BtsI	GCAGTG	2 cut(s)	417, 1693
BtsIMutI	CAGTG	5 cut(s)	231, 417, 1585, 1693, 1876
BtuMI	TCGCGA	1 cut(s)	888
Cac8I	GCNNGC	7 cut(s)	408, 417, 601, 831, 862, 1043, 1560
CaiI	CAGNNNCTG	2 cut(s)	416, 479
CciI	TCATGA	1 cut(s)	166
CfoI	GCGC	2 cut(s)	373, 1041
Cfr10I	RCCGGY	2 cut(s)	337, 1430
Cfr13I	GGNCC	2 cut(s)	526, 1052
ClaI	ATCGAT	1 cut(s)	707
Csp6I	GTAC	1 cut(s)	514
CspAI	ACCGGT	1 cut(s)	337
CspCI	CAANNNNNGTGG	2 cut(s)	120, 155
CviAII	CATG	7 cut(s)	167, 440, 542, 600, 743, 1160, 1822
CviQI	GTAC	1 cut(s)	514
DdeI	CTNAG	2 cut(s)	1131, 1391
DpnI	GATC	9 cut(s)	76, 354, 891, 1142, 1167, 1185, 1647, 1766, 1779
DpnII	GATC	9 cut(s)	74, 352, 889, 1140, 1165, 1183, 1645, 1764, 1777
DraI	TTTAAA	2 cut(s)	1437, 1626
Eam1104I	CTCTTC	3 cut(s)	270, 1143, 1752
EarI	CTCTTC	3 cut(s)	270, 1143, 1752
Ecl136II	GAGCTC	1 cut(s)	775
Eco130I	CCWWGG	2 cut(s)	27, 1286
Eco24I	GRGCYC	2 cut(s)	218, 777
Eco31I	GGTCTC	3 cut(s)	55, 353, 415
Eco47I	GGWCC	1 cut(s)	1052
Eco53kI	GAGCTC	1 cut(s)	775
Eco57I	CTGAAG	3 cut(s)	452, 1050, 1437
Eco88I	CYCGRG	1 cut(s)	899
EcoICRI	GAGCTC	1 cut(s)	775
EcoRII	CCWGG	2 cut(s)	979, 1187
EcoT14I	CCWWGG	2 cut(s)	27, 1286
EcoT22I	ATGCAT	2 cut(s)	678, 885
EcoT38I	GRGCYC	2 cut(s)	218, 777
ErhI	CCWWGG	2 cut(s)	27, 1286
FaeI	CATG	7 cut(s)	170, 443, 545, 603, 746, 1163, 1825
FalI	AAGNNNNNCTT	2 cut(s)	1695, 1727
FaqI	GGGAC	2 cut(s)	409, 568
FatI	CATG	7 cut(s)	166, 439, 541, 599, 742, 1159, 1821
Fnu4HI	GCNGC	9 cut(s)	230, 294, 303, 717, 822, 1256, 1563, 1611, 1659
FokI	GGATG	4 cut(s)	243, 662, 740, 1757
FriOI	GRGCYC	2 cut(s)	218, 777
Fsp4HI	GCNGC	9 cut(s)	230, 294, 303, 717, 822, 1256, 1563, 1611, 1659
FspBI	CTAG	2 cut(s)	105, 830
FspI	TGCGCA	1 cut(s)	1040
GlaI	GCGC	2 cut(s)	372, 1040
GluI	GCNGC	9 cut(s)	230, 294, 303, 717, 822, 1256, 1563, 1611, 1659
GsuI	CTGGAG	1 cut(s)	1296
HaeIII	GGCC	2 cut(s)	528, 1192
HapII	CCGG	4 cut(s)	81, 338, 585, 1431
HhaI	GCGC	2 cut(s)	373, 1041
Hin1II	CATG	7 cut(s)	170, 443, 545, 603, 746, 1163, 1825
Hin6I	GCGC	2 cut(s)	371, 1039
HinP1I	GCGC	2 cut(s)	371, 1039
HincII	GTYRAC	1 cut(s)	1573
HindII	GTYRAC	1 cut(s)	1573
HindIII	AAGCTT	2 cut(s)	533, 858
HinfI	GANTC	8 cut(s)	70, 476, 664, 691, 932, 1328, 1395, 1535
HpaII	CCGG	4 cut(s)	81, 338, 585, 1431
HphI	GGTGA	5 cut(s)	782, 995, 1136, 1175, 1274
Hpy166II	GTNNAC	3 cut(s)	69, 1573, 1750
Hpy188I	TCNGA	8 cut(s)	154, 312, 811, 1204, 1241, 1417, 1540, 1769
Hpy8I	GTNNAC	3 cut(s)	69, 1573, 1750
Hpy99I	CGWCG	1 cut(s)	250
HpyAV	CCTTC	4 cut(s)	244, 307, 502, 1849
HpyCH4III	ACNGT	2 cut(s)	14, 1580
HpyF10VI	GCNNNNNNNGC	6 cut(s)	299, 302, 412, 416, 830, 944
HpyF3I	CTNAG	2 cut(s)	1131, 1391
Hsp92II	CATG	7 cut(s)	170, 443, 545, 603, 746, 1163, 1825
HspAI	GCGC	2 cut(s)	371, 1039
Kzo9I	GATC	9 cut(s)	74, 352, 889, 1140, 1165, 1183, 1645, 1764, 1777
LmnI	GCTCC	2 cut(s)	1315, 1520
Lsp1109I	GCAGC	7 cut(s)	241, 305, 314, 833, 1549, 1622, 1670
LweI	GCATC	7 cut(s)	115, 685, 718, 892, 1221, 1545, 1815
MaeI	CTAG	2 cut(s)	105, 830
MaeIII	GTNAC	2 cut(s)	91, 1280
MalI	GATC	9 cut(s)	76, 354, 891, 1142, 1167, 1185, 1647, 1766, 1779
MboI	GATC	9 cut(s)	74, 352, 889, 1140, 1165, 1183, 1645, 1764, 1777
MfeI	CAATTG	1 cut(s)	1587
MflI	RGATCY	2 cut(s)	1140, 1183
MhlI	GDGCHC	3 cut(s)	218, 777, 1876
MlyI	GAGTC	4 cut(s)	64, 485, 1404, 1544
MmeI	TCCRAC	2 cut(s)	826, 985
Mph1103I	ATGCAT	2 cut(s)	678, 885
MroXI	GAANNNNTTC	1 cut(s)	1355
MseI	TTAA	8 cut(s)	114, 711, 894, 1340, 1377, 1436, 1625, 1912
MslI	CAYNNNNRTG	1 cut(s)	64
MspCI	CTTAAG	1 cut(s)	1339
MspI	CCGG	4 cut(s)	81, 338, 585, 1431
MspR9I	CCNGG	3 cut(s)	82, 981, 1189
MunI	CAATTG	1 cut(s)	1587
Mva1269I	GAATGC	1 cut(s)	1737
MvaI	CCWGG	2 cut(s)	981, 1189
MvnI	CGCG	1 cut(s)	888
MwoI	GCNNNNNNNGC	6 cut(s)	299, 302, 412, 416, 830, 944
NciI	CCSGG	1 cut(s)	82
NdeII	GATC	9 cut(s)	74, 352, 889, 1140, 1165, 1183, 1645, 1764, 1777
NheI	GCTAGC	1 cut(s)	829
NlaIII	CATG	7 cut(s)	170, 443, 545, 603, 746, 1163, 1825
NlaIV	GGNNCC	2 cut(s)	1110, 1311
NmeAIII	GCCGAG	1 cut(s)	805
NmuCI	GTSAC	2 cut(s)	91, 1280
NruI	TCGCGA	1 cut(s)	888
NsbI	TGCGCA	1 cut(s)	1040
NsiI	ATGCAT	2 cut(s)	678, 885
NspI	RCATGY	1 cut(s)	603
NspV	TTCGAA	1 cut(s)	1488
OliI	CACNNNNGTG	1 cut(s)	64
PaeI	GCATGC	1 cut(s)	603
PagI	TCATGA	1 cut(s)	166
PctI	GAATGC	1 cut(s)	1737
PdmI	GAANNNNTTC	1 cut(s)	1355
PfeI	GAWTC	4 cut(s)	664, 691, 932, 1328
PflMI	CCANNNNNTGG	1 cut(s)	871
PinAI	ACCGGT	1 cut(s)	337
PkrI	GCNGC	9 cut(s)	231, 295, 304, 718, 823, 1257, 1564, 1612, 1660
PleI	GAGTC	4 cut(s)	64, 484, 1403, 1543
PpsI	GAGTC	4 cut(s)	64, 484, 1403, 1543
Psp124BI	GAGCTC	1 cut(s)	777
Psp6I	CCWGG	2 cut(s)	979, 1187
PspGI	CCWGG	2 cut(s)	979, 1187
PspN4I	GGNNCC	2 cut(s)	1110, 1311
PspPI	GGNCC	2 cut(s)	526, 1052
PstI	CTGCAG	1 cut(s)	1336
PstNI	CAGNNNCTG	2 cut(s)	416, 479
PsuI	RGATCY	2 cut(s)	1140, 1183
RruI	TCGCGA	1 cut(s)	888
RsaI	GTAC	1 cut(s)	515
RsaNI	GTAC	1 cut(s)	514
RseI	CAYNNNNRTG	1 cut(s)	64
SacI	GAGCTC	1 cut(s)	777
SaqAI	TTAA	8 cut(s)	114, 711, 894, 1340, 1377, 1436, 1625, 1912
SatI	GCNGC	9 cut(s)	230, 294, 303, 717, 822, 1256, 1563, 1611, 1659
Sau3AI	GATC	9 cut(s)	74, 352, 889, 1140, 1165, 1183, 1645, 1764, 1777
Sau96I	GGNCC	2 cut(s)	526, 1052
ScaI	AGTACT	1 cut(s)	515
SchI	GAGTC	4 cut(s)	64, 485, 1404, 1544
ScrFI	CCNGG	3 cut(s)	82, 981, 1189
SduI	GDGCHC	3 cut(s)	218, 777, 1876
SfaNI	GCATC	7 cut(s)	115, 685, 718, 892, 1221, 1545, 1815
SfcI	CTRYAG	3 cut(s)	840, 948, 1332
SfuI	TTCGAA	1 cut(s)	1488
SinI	GGWCC	1 cut(s)	1052
SmiMI	CAYNNNNRTG	1 cut(s)	64
SmlI	CTYRAG	2 cut(s)	1339, 1701
SmoI	CTYRAG	2 cut(s)	1339, 1701
SphI	GCATGC	1 cut(s)	603
SsiI	CCGC	3 cut(s)	716, 1045, 1255
SspI	AATATT	2 cut(s)	969, 1198
SspMI	CTAG	2 cut(s)	105, 830
SstI	GAGCTC	1 cut(s)	777
StyD4I	CCNGG	3 cut(s)	80, 979, 1187
StyI	CCWWGG	2 cut(s)	27, 1286
TaaI	ACNGT	2 cut(s)	14, 1580
TaqI	TCGA	6 cut(s)	73, 455, 707, 1488, 1776, 1795
TaqII	GACCGA	2 cut(s)	37, 439
TatI	WGTACW	1 cut(s)	513
TauI	GCSGC	2 cut(s)	719, 1258
TfiI	GAWTC	4 cut(s)	664, 691, 932, 1328
Tru1I	TTAA	8 cut(s)	114, 711, 894, 1340, 1377, 1436, 1625, 1912
Tru9I	TTAA	8 cut(s)	114, 711, 894, 1340, 1377, 1436, 1625, 1912
TscAI	CASTG	5 cut(s)	231, 417, 1585, 1693, 1876
TseFI	GTSAC	2 cut(s)	91, 1280
TseI	GCWGC	7 cut(s)	229, 293, 302, 821, 1562, 1610, 1658
Tsp45I	GTSAC	2 cut(s)	91, 1280
TspDTI	ATGAA	5 cut(s)	245, 731, 1382, 1594, 1722
TspGWI	ACGGA	1 cut(s)	224
TspRI	CASTG	5 cut(s)	231, 417, 1585, 1693, 1876
Van91I	CCANNNNNTGG	1 cut(s)	871
Vha464I	CTTAAG	1 cut(s)	1339
VpaK11BI	GGWCC	1 cut(s)	1052
XapI	RAATTY	2 cut(s)	22, 55
XceI	RCATGY	1 cut(s)	603
XmnI	GAANNNNTTC	1 cut(s)	1355
XspI	CTAG	2 cut(s)	105, 830
ZrmI	AGTACT	1 cut(s)	515
Zsp2I	ATGCAT	2 cut(s)	678, 885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.