Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0002410

Physical Location & Seq

Genomic Coordinates Reverse (-)

27682484 .. 27685920

Gene Structure 3437 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023739

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1947 bp

ATGGTTCTTGAGGAATTTCTGTGCCTTGGGAAGATGGGAAACAACTGTGTTGGAAATTCCAAGGACGGGCTTTTCCATTCGGTCTCAAATTCACTATGGTGGACTCGATCAACCGGGGGTTTGGTTACTTCTACAACTAGAGAAATTAAAAGTAGTGATGCACCACCATCATTGAACAATCAGTCAGAAACTAATCCTCATGATGTTCAAAGCAATCCCCCAGAAGCAATCAAGATGACTACGGAGGGCTCTAAACCAGTGCAGCCCCCAATTCAACCGACGAAGGTTCATCCTGAAAAGCAAAAGGAAGAGGAGACTAAACCAGCAGCACAAGTAGCAATTCCGAAGGAAGAGACTAAACCTCAACAACCGGTAAATCATAGGGATCAAGAGACCAGACCGGTGCAACCCATAACACAAAATGGAGGGACTAATCAGCCAGCAGTGCCTGCGAGACCGAAGAAGCCCCATAATGTCAAGAGGGTATCGAGTGCAGGGCTTCAGGCAGAGTCTGTCTTGCAGACCAAGACAGGTCATTTGAAGGAGTACTACAATTTGGGCCAGAAGCTTGGACATGGACAATTTGGGACTACTTTCCTTTGTGTGGAGAAAGCAACCGGAAAGGAATACGCATGCAAATCCATTGCCAAAAGGAAATTGTTGGCAATAGAAGATGTGGAGGATGTGAGGAGGGAGATTCAAATAATGCATCACTTGGCAGGGAATCCCAATGTCATATCAATTAAGGCGGCTTATGAGGATGCTGTTGCAGTTCATGTTGTTATGGAATTATGTGCAGGGGGTGAGCTGTTTGATAGGATTATCAAGAGGGGGCATTACTCTGAAAGAAAGGCAGCTCGGCTAGCAAGGACTATAGTTGGAGTTATACAAGCTTGCCATTCTTTGGGTGTTATGCATCGCGATCTTAAACCTGAGAACTTTCTTTTTGTTGATGAGGAGGAGGATTCAGCACTCAAAGCAATAGATTTTGGGCTGTCAATATTTTTCAAGCCAGGTGAAACTTTCAATGATGTGGTTGGAAGCCCATATTATGTTGCACCTGAAGTTCTGCGCAAGCGGTATGGTCCAGAAGCAGATGTTTGGAGTGTTGGTGTTATCATTTACATTCTCTTGAGTGGGGTGCCTCCATTCTGGGGTGAAACTGAGCAAGAGATCTTTGAAGAGGTTTTGCATGGTGATCTTGACTTCTCAACAGATCCCTGGCCTAATATTTCAGAAAGTGCAAAAGATTTAGTCAAGAAGATGCTTGTCAGAAACCCCAAAAAGCGGCTAACTGCTCATCAAGTTCTATGTCACCCTTGGGTTCAGGTTGATGGAGTGGCTCCAGACAAGCCTCTTGATTCTGCAGTCTTAAGTCGCTTGAAGCAGTTTTCTGCAATGAACAAAATTAAGAAAATGGCTCTTAGAGTCATTGCTGAAAACCTCTCTGAAGAAGAAATTGCCGGATTAAAAGAAATGTTCAAGATGATTGATACTGACAATAGTGGTCAAATTAGTTTCGAAGAACTGAAAGATGGACTGAAGAGATTTGGAGCTACTCTAAATGAGTCTGAAATATACGACCTGATGCAAGCCGCTGATGTTGACAACAGTGGCACAATTGATTATGGGGAGTTCATAGCAGCAACATTGCATTTAAACAAAGTAGAGAGGGAAGATCATTTGTTTGCAGCTTTCTCATATTTTGACAAAGATGGCAGTGGCTATATAACTCAAGATGAACTTCAACAAGCGTGTGAGGAGTTTGGCATTCAGGATGTCCACTTGGAAGAGTTGATCAGAGAAGTCGATCAGGACAATGATGGTCGAATAGATTACAATGAGTTCGTTGCCATGATGCAGAAAGGCAATCCAGAGTTTGATAAGAAGGGTATTCAAACCAGTGCTCTTGGCATTGGATTTAGGGAGGCACTATCTGTTTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031984 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0098827 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

649

Amino Acids

72.23

Weight (kDa)

5.42

Isoelectric Point (pI)

44.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	184 - 442	1.3e-76	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	186 - 436	1.2e-23	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	490 - 516	1.1e-08	EF hand domain
EF-hand_7	PF13499	490 - 549	8.6e-13	EF-hand domain pair
EF-hand_6	PF13405	490 - 518	4.4e-07	EF-hand domain
EF-hand_5	PF13202	490 - 512	2.8e-06	EF hand
EF-hand_8	PF13833	504 - 550	6.1e-07	EF-hand domain pair
EF-hand_7	PF13499	559 - 621	2.3e-16	EF-hand domain pair
EF-hand_1	PF00036	563 - 588	3.9e-06	EF hand domain
EF-hand_6	PF13405	563 - 590	6.2e-07	EF-hand domain
EF-hand_1	PF00036	596 - 622	4.6e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10660 AT3G10660 AT5G04870
fragaria_vesca	FvH4_6g20840 FvH4_6g20840 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490
malus_domestica	MD02G1257300.v1.1 MD07G1063300.v1.1 MD12G1044400.v1.1 MD14G1043300.v1.1
prunus_persica	Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.7G090800_v2.0.a1 Prupe.7G090800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0332341 RchiOBHm_Chr3g0464211 RchiOBHm_Chr3g0477181
rosa_laevigata	RLG00000023739 RLG00000029678
rosa_multiflora	Rmu_sc0000932.1_g000001 Rmu_sc0003422.1_g000006
rosa_roxburghii	Rroxscaffold_4G00318280 Rroxscaffold_6G00404350 Rroxscaffold_6G00404850
rosa_rugosa	Rorug01G0100000 Rorug03G0158600
rosa_samantha	Rh1CG118000 Rh1DG129100 Rh3AG208900 Rh3CG236600 Rh3DG235400
rosa_wichuraiana	Rw1G010080 Rw3G019050 Rw3G019440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1073
AccB1I	GGYRCC	1 cut(s)	1141
AccB7I	CCANNNNNTGG	1 cut(s)	904
AccII	CGCG	1 cut(s)	921
AciI	CCGC	4 cut(s)	749, 1078, 1288, 1596
AclWI	GGATC	2 cut(s)	393, 1211
AcsI	RAATTY	3 cut(s)	14, 55, 88
AcuI	CTGAAG	4 cut(s)	485, 1083, 1470, 1562
AfaI	GTAC	1 cut(s)	548
AfiI	CCNNNNNNNGG	5 cut(s)	66, 604, 904, 1154, 1287
AflII	CTTAAG	1 cut(s)	1372
AgeI	ACCGGT	2 cut(s)	370, 400
AjnI	CCWGG	2 cut(s)	1012, 1220
AleI	CACNNNNGTG	1 cut(s)	97
AluBI	AGCT	6 cut(s)	568, 808, 857, 893, 1556, 1694
AluI	AGCT	6 cut(s)	568, 808, 857, 893, 1556, 1694
Alw21I	GWGCWC	1 cut(s)	1909
Alw26I	GTCTC	5 cut(s)	88, 308, 347, 386, 448
AlwI	GGATC	2 cut(s)	393, 1211
AlwNI	CAGNNNCTG	2 cut(s)	449, 512
AoxI	GGCC	2 cut(s)	559, 1223
ApeKI	GCWGC	5 cut(s)	262, 326, 854, 1643, 1691
ApoI	RAATTY	3 cut(s)	14, 55, 88
ArsI	GACNNNNNNTTYG	2 cut(s)	520, 552
AsiGI	ACCGGT	2 cut(s)	370, 400
AspLEI	GCGC	1 cut(s)	1074
AspS9I	GGNCC	2 cut(s)	559, 1085
AsuC2I	CCSGG	1 cut(s)	115
AsuHPI	GGTGA	5 cut(s)	815, 1028, 1169, 1208, 1307
AsuII	TTCGAA	1 cut(s)	1521
AsuNHI	GCTAGC	1 cut(s)	862
AvaII	GGWCC	1 cut(s)	1085
BanI	GGYRCC	1 cut(s)	1141
BanII	GRGCYC	1 cut(s)	251
BarI	GAAGNNNNNNTAC	2 cut(s)	533, 565
Bbv12I	GWGCWC	1 cut(s)	1909
BbvI	GCAGC	5 cut(s)	274, 338, 866, 1655, 1703
BccI	CCATC	6 cut(s)	28, 175, 1328, 1529, 1709, 1817
BciT130I	CCWGG	2 cut(s)	1014, 1222
BclI	TGATCA	1 cut(s)	1797
BcnI	CCSGG	1 cut(s)	115
BcoDI	GTCTC	5 cut(s)	88, 308, 347, 386, 448
BfaI	CTAG	2 cut(s)	138, 863
BfmI	CTRYAG	2 cut(s)	873, 1365
BfrI	CTTAAG	1 cut(s)	1372
BglII	AGATCT	1 cut(s)	1173
BisI	GCNGC	8 cut(s)	263, 327, 750, 855, 1289, 1596, 1644, 1692
BlsI	GCNGC	8 cut(s)	264, 328, 751, 856, 1290, 1597, 1645, 1693
BmcAI	AGTACT	1 cut(s)	548
Bme1390I	CCNGG	3 cut(s)	115, 1014, 1222
Bme18I	GGWCC	1 cut(s)	1085
BmgT120I	GGNCC	2 cut(s)	559, 1085
BmiI	GGNNCC	2 cut(s)	1143, 1344
BmrFI	CCNGG	3 cut(s)	115, 1014, 1222
BmsI	GCATC	7 cut(s)	148, 718, 751, 925, 1254, 1578, 1848
BmtI	GCTAGC	1 cut(s)	866
BpmI	CTGGAG	1 cut(s)	1329
Bpu14I	TTCGAA	1 cut(s)	1521
BpuEI	CTTGAG	3 cut(s)	29, 1153, 1719
BpuMI	CCSGG	1 cut(s)	115
BsaI	GGTCTC	3 cut(s)	88, 386, 448
BsaJI	CCNNGG	5 cut(s)	25, 60, 114, 1220, 1319
BsaWI	WCCGGW	3 cut(s)	370, 400, 617
BsaXI	ACNNNNNCTCC	2 cut(s)	1096, 1126
Bsc4I	CCNNNNNNNGG	5 cut(s)	66, 604, 904, 1154, 1287
Bse118I	RCCGGY	2 cut(s)	370, 400
Bse1I	ACTGG	2 cut(s)	257, 1902
Bse3DI	GCAATG	4 cut(s)	642, 1404, 1431, 1649
BseBI	CCWGG	2 cut(s)	1014, 1222
BseDI	CCNNGG	5 cut(s)	25, 60, 114, 1220, 1319
BseGI	GGATG	4 cut(s)	289, 688, 766, 1783
BseLI	CCNNNNNNNGG	5 cut(s)	66, 604, 904, 1154, 1287
BseMI	GCAATG	4 cut(s)	642, 1404, 1431, 1649
BseMII	CTCAG	2 cut(s)	924, 1155
BseNI	ACTGG	2 cut(s)	257, 1902
BseRI	GAGGAG	5 cut(s)	326, 703, 971, 974, 1775
BseXI	GCAGC	5 cut(s)	274, 338, 866, 1655, 1703
BsgI	GTGCAG	3 cut(s)	281, 513, 816
Bsh1236I	CGCG	1 cut(s)	921
BshFI	GGCC	2 cut(s)	561, 1225
BshNI	GGYRCC	1 cut(s)	1141
BshTI	ACCGGT	2 cut(s)	370, 400
BsiHKAI	GWGCWC	1 cut(s)	1909
BsiSI	CCGG	5 cut(s)	114, 371, 401, 618, 1464
BslFI	GGGAC	2 cut(s)	442, 601
BslI	CCNNNNNNNGG	5 cut(s)	66, 604, 904, 1154, 1287
BsmAI	GTCTC	5 cut(s)	88, 308, 347, 386, 448
BsmFI	GGGAC	2 cut(s)	442, 601
BsmI	GAATGC	1 cut(s)	1770
BsnI	GGCC	2 cut(s)	561, 1225
Bso31I	GGTCTC	3 cut(s)	88, 386, 448
Bsp119I	TTCGAA	1 cut(s)	1521
Bsp1286I	GDGCHC	2 cut(s)	251, 1909
Bsp143I	GATC	9 cut(s)	107, 385, 922, 1173, 1198, 1216, 1678, 1797, 1810
Bsp68I	TCGCGA	1 cut(s)	921
BspACI	CCGC	4 cut(s)	749, 1078, 1288, 1596
BspANI	GGCC	2 cut(s)	561, 1225
BspCNI	CTCAG	2 cut(s)	925, 1156
BspFNI	CGCG	1 cut(s)	921
BspHI	TCATGA	1 cut(s)	199
BspLI	GGNNCC	2 cut(s)	1143, 1344
BspMAI	CTGCAG	1 cut(s)	1369
BspOI	GCTAGC	1 cut(s)	866
BspPI	GGATC	2 cut(s)	393, 1211
BspT104I	TTCGAA	1 cut(s)	1521
BspT107I	GGYRCC	1 cut(s)	1141
BspTI	CTTAAG	1 cut(s)	1372
BspTNI	GGTCTC	3 cut(s)	88, 386, 448
BsrDI	GCAATG	4 cut(s)	642, 1404, 1431, 1649
BsrFI	RCCGGY	2 cut(s)	370, 400
BsrI	ACTGG	2 cut(s)	257, 1902
BssAI	RCCGGY	2 cut(s)	370, 400
BssECI	CCNNGG	5 cut(s)	25, 60, 114, 1220, 1319
BssMI	GATC	9 cut(s)	107, 385, 922, 1173, 1198, 1216, 1678, 1797, 1810
BssT1I	CCWWGG	3 cut(s)	25, 60, 1319
Bst2UI	CCWGG	2 cut(s)	1014, 1222
Bst4CI	ACNGT	2 cut(s)	47, 1613
Bst6I	CTCTTC	5 cut(s)	303, 345, 1176, 1538, 1785
BstAFI	CTTAAG	1 cut(s)	1372
BstAPI	GCANNNNNTGC	1 cut(s)	449
BstBI	TTCGAA	1 cut(s)	1521
BstC8I	GCNNGC	7 cut(s)	441, 450, 634, 864, 895, 1076, 1593
BstDEI	CTNAG	3 cut(s)	933, 1164, 1424
BstF5I	GGATG	4 cut(s)	289, 688, 766, 1783
BstFNI	CGCG	1 cut(s)	921
BstHHI	GCGC	1 cut(s)	1074
BstKTI	GATC	9 cut(s)	110, 388, 925, 1176, 1201, 1219, 1681, 1800, 1813
BstMAI	GTCTC	5 cut(s)	88, 308, 347, 386, 448
BstMBI	GATC	9 cut(s)	107, 385, 922, 1173, 1198, 1216, 1678, 1797, 1810
BstMWI	GCNNNNNNNGC	5 cut(s)	335, 445, 449, 863, 977
BstNI	CCWGG	2 cut(s)	1014, 1222
BstNSI	RCATGY	1 cut(s)	636
BstSCI	CCNGG	3 cut(s)	113, 1012, 1220
BstSFI	CTRYAG	2 cut(s)	873, 1365
BstUI	CGCG	1 cut(s)	921
BstV1I	GCAGC	5 cut(s)	274, 338, 866, 1655, 1703
BstX2I	RGATCY	2 cut(s)	1173, 1216
BstXI	CCANNNNNNTGG	1 cut(s)	569
BstYI	RGATCY	2 cut(s)	1173, 1216
BsuRI	GGCC	2 cut(s)	561, 1225
BtgZI	GCGATG	1 cut(s)	902
BtsCI	GGATG	4 cut(s)	289, 688, 766, 1783
BtsI	GCAGTG	2 cut(s)	450, 1726
BtsIMutI	CAGTG	5 cut(s)	264, 450, 1618, 1726, 1909
BtuMI	TCGCGA	1 cut(s)	921
Cac8I	GCNNGC	7 cut(s)	441, 450, 634, 864, 895, 1076, 1593
CaiI	CAGNNNCTG	2 cut(s)	449, 512
CciI	TCATGA	1 cut(s)	199
CfoI	GCGC	1 cut(s)	1074
Cfr10I	RCCGGY	2 cut(s)	370, 400
Cfr13I	GGNCC	2 cut(s)	559, 1085
Csp6I	GTAC	1 cut(s)	547
CspAI	ACCGGT	2 cut(s)	370, 400
CspCI	CAANNNNNGTGG	2 cut(s)	153, 188
CviAII	CATG	6 cut(s)	200, 575, 633, 776, 1193, 1855
CviQI	GTAC	1 cut(s)	547
DdeI	CTNAG	3 cut(s)	933, 1164, 1424
DpnI	GATC	9 cut(s)	109, 387, 924, 1175, 1200, 1218, 1680, 1799, 1812
DpnII	GATC	9 cut(s)	107, 385, 922, 1173, 1198, 1216, 1678, 1797, 1810
DraI	TTTAAA	1 cut(s)	1659
Eam1104I	CTCTTC	5 cut(s)	303, 345, 1176, 1538, 1785
EarI	CTCTTC	5 cut(s)	303, 345, 1176, 1538, 1785
Eco130I	CCWWGG	3 cut(s)	25, 60, 1319
Eco24I	GRGCYC	1 cut(s)	251
Eco31I	GGTCTC	3 cut(s)	88, 386, 448
Eco47I	GGWCC	1 cut(s)	1085
Eco57I	CTGAAG	4 cut(s)	485, 1083, 1470, 1562
EcoRII	CCWGG	2 cut(s)	1012, 1220
EcoT14I	CCWWGG	3 cut(s)	25, 60, 1319
EcoT22I	ATGCAT	2 cut(s)	711, 918
EcoT38I	GRGCYC	1 cut(s)	251
ErhI	CCWWGG	3 cut(s)	25, 60, 1319
FaeI	CATG	6 cut(s)	203, 578, 636, 779, 1196, 1858
FalI	AAGNNNNNCTT	2 cut(s)	1728, 1760
FaqI	GGGAC	2 cut(s)	442, 601
FatI	CATG	6 cut(s)	199, 574, 632, 775, 1192, 1854
FbaI	TGATCA	1 cut(s)	1797
Fnu4HI	GCNGC	8 cut(s)	263, 327, 750, 855, 1289, 1596, 1644, 1692
FokI	GGATG	4 cut(s)	276, 695, 773, 1790
FriOI	GRGCYC	1 cut(s)	251
Fsp4HI	GCNGC	8 cut(s)	263, 327, 750, 855, 1289, 1596, 1644, 1692
FspBI	CTAG	2 cut(s)	138, 863
FspI	TGCGCA	1 cut(s)	1073
GlaI	GCGC	1 cut(s)	1073
GluI	GCNGC	8 cut(s)	263, 327, 750, 855, 1289, 1596, 1644, 1692
GsuI	CTGGAG	1 cut(s)	1329
HaeIII	GGCC	2 cut(s)	561, 1225
HapII	CCGG	5 cut(s)	114, 371, 401, 618, 1464
HhaI	GCGC	1 cut(s)	1074
Hin1II	CATG	6 cut(s)	203, 578, 636, 779, 1196, 1858
Hin6I	GCGC	1 cut(s)	1072
HinP1I	GCGC	1 cut(s)	1072
HincII	GTYRAC	1 cut(s)	1606
HindII	GTYRAC	1 cut(s)	1606
HindIII	AAGCTT	2 cut(s)	566, 891
HinfI	GANTC	8 cut(s)	103, 509, 697, 724, 965, 1361, 1428, 1568
HpaII	CCGG	5 cut(s)	114, 371, 401, 618, 1464
HphI	GGTGA	5 cut(s)	815, 1028, 1169, 1208, 1307
Hpy166II	GTNNAC	3 cut(s)	102, 1606, 1783
Hpy188I	TCNGA	8 cut(s)	187, 345, 844, 1237, 1274, 1450, 1573, 1802
Hpy8I	GTNNAC	3 cut(s)	102, 1606, 1783
Hpy99I	CGWCG	1 cut(s)	283
HpyAV	CCTTC	4 cut(s)	277, 340, 535, 1882
HpyCH4III	ACNGT	2 cut(s)	47, 1613
HpyF10VI	GCNNNNNNNGC	5 cut(s)	335, 445, 449, 863, 977
HpyF3I	CTNAG	3 cut(s)	933, 1164, 1424
Hsp92II	CATG	6 cut(s)	203, 578, 636, 779, 1196, 1858
HspAI	GCGC	1 cut(s)	1072
Ksp22I	TGATCA	1 cut(s)	1797
Kzo9I	GATC	9 cut(s)	107, 385, 922, 1173, 1198, 1216, 1678, 1797, 1810
LmnI	GCTCC	2 cut(s)	1348, 1553
Lsp1109I	GCAGC	5 cut(s)	274, 338, 866, 1655, 1703
LweI	GCATC	7 cut(s)	148, 718, 751, 925, 1254, 1578, 1848
MaeI	CTAG	2 cut(s)	138, 863
MaeIII	GTNAC	2 cut(s)	124, 1313
MalI	GATC	9 cut(s)	109, 387, 924, 1175, 1200, 1218, 1680, 1799, 1812
MboI	GATC	9 cut(s)	107, 385, 922, 1173, 1198, 1216, 1678, 1797, 1810
MfeI	CAATTG	1 cut(s)	1620
MflI	RGATCY	2 cut(s)	1173, 1216
MhlI	GDGCHC	2 cut(s)	251, 1909
MlyI	GAGTC	4 cut(s)	97, 518, 1437, 1577
MmeI	TCCRAC	3 cut(s)	31, 859, 1018
Mph1103I	ATGCAT	2 cut(s)	711, 918
MseI	TTAA	8 cut(s)	147, 744, 927, 1373, 1410, 1469, 1658, 1945
MslI	CAYNNNNRTG	1 cut(s)	97
MspA1I	CMGCKG	1 cut(s)	1598
MspCI	CTTAAG	1 cut(s)	1372
MspI	CCGG	5 cut(s)	114, 371, 401, 618, 1464
MspR9I	CCNGG	3 cut(s)	115, 1014, 1222
MunI	CAATTG	1 cut(s)	1620
Mva1269I	GAATGC	1 cut(s)	1770
MvaI	CCWGG	2 cut(s)	1014, 1222
MvnI	CGCG	1 cut(s)	921
MwoI	GCNNNNNNNGC	5 cut(s)	335, 445, 449, 863, 977
NciI	CCSGG	1 cut(s)	115
NdeII	GATC	9 cut(s)	107, 385, 922, 1173, 1198, 1216, 1678, 1797, 1810
NheI	GCTAGC	1 cut(s)	862
NlaIII	CATG	6 cut(s)	203, 578, 636, 779, 1196, 1858
NlaIV	GGNNCC	2 cut(s)	1143, 1344
NmeAIII	GCCGAG	1 cut(s)	838
NmuCI	GTSAC	1 cut(s)	1313
NruI	TCGCGA	1 cut(s)	921
NsbI	TGCGCA	1 cut(s)	1073
NsiI	ATGCAT	2 cut(s)	711, 918
NspI	RCATGY	1 cut(s)	636
NspV	TTCGAA	1 cut(s)	1521
OliI	CACNNNNGTG	1 cut(s)	97
PaeI	GCATGC	1 cut(s)	636
PagI	TCATGA	1 cut(s)	199
PctI	GAATGC	1 cut(s)	1770
PfeI	GAWTC	4 cut(s)	697, 724, 965, 1361
PflMI	CCANNNNNTGG	1 cut(s)	904
PinAI	ACCGGT	2 cut(s)	370, 400
PkrI	GCNGC	8 cut(s)	264, 328, 751, 856, 1290, 1597, 1645, 1693
PleI	GAGTC	4 cut(s)	97, 517, 1436, 1576
PpsI	GAGTC	4 cut(s)	97, 517, 1436, 1576
Psp6I	CCWGG	2 cut(s)	1012, 1220
PspGI	CCWGG	2 cut(s)	1012, 1220
PspN4I	GGNNCC	2 cut(s)	1143, 1344
PspPI	GGNCC	2 cut(s)	559, 1085
PstI	CTGCAG	1 cut(s)	1369
PstNI	CAGNNNCTG	2 cut(s)	449, 512
PsuI	RGATCY	2 cut(s)	1173, 1216
RruI	TCGCGA	1 cut(s)	921
RsaI	GTAC	1 cut(s)	548
RsaNI	GTAC	1 cut(s)	547
RseI	CAYNNNNRTG	1 cut(s)	97
SaqAI	TTAA	8 cut(s)	147, 744, 927, 1373, 1410, 1469, 1658, 1945
SatI	GCNGC	8 cut(s)	263, 327, 750, 855, 1289, 1596, 1644, 1692
Sau3AI	GATC	9 cut(s)	107, 385, 922, 1173, 1198, 1216, 1678, 1797, 1810
Sau96I	GGNCC	2 cut(s)	559, 1085
ScaI	AGTACT	1 cut(s)	548
SchI	GAGTC	4 cut(s)	97, 518, 1437, 1577
ScrFI	CCNGG	3 cut(s)	115, 1014, 1222
SduI	GDGCHC	2 cut(s)	251, 1909
SfaNI	GCATC	7 cut(s)	148, 718, 751, 925, 1254, 1578, 1848
SfcI	CTRYAG	2 cut(s)	873, 1365
SfuI	TTCGAA	1 cut(s)	1521
SinI	GGWCC	1 cut(s)	1085
SmiMI	CAYNNNNRTG	1 cut(s)	97
SmlI	CTYRAG	4 cut(s)	8, 1132, 1372, 1734
SmoI	CTYRAG	4 cut(s)	8, 1132, 1372, 1734
SphI	GCATGC	1 cut(s)	636
SsiI	CCGC	4 cut(s)	749, 1078, 1288, 1596
SspI	AATATT	2 cut(s)	1002, 1231
SspMI	CTAG	2 cut(s)	138, 863
StyD4I	CCNGG	3 cut(s)	113, 1012, 1220
StyI	CCWWGG	3 cut(s)	25, 60, 1319
TaaI	ACNGT	2 cut(s)	47, 1613
TaqI	TCGA	5 cut(s)	106, 488, 1521, 1809, 1828
TaqII	GACCGA	2 cut(s)	70, 472
TatI	WGTACW	1 cut(s)	546
TauI	GCSGC	3 cut(s)	752, 1291, 1598
TfiI	GAWTC	4 cut(s)	697, 724, 965, 1361
Tru1I	TTAA	8 cut(s)	147, 744, 927, 1373, 1410, 1469, 1658, 1945
Tru9I	TTAA	8 cut(s)	147, 744, 927, 1373, 1410, 1469, 1658, 1945
TscAI	CASTG	5 cut(s)	264, 450, 1618, 1726, 1909
TseFI	GTSAC	1 cut(s)	1313
TseI	GCWGC	5 cut(s)	262, 326, 854, 1643, 1691
Tsp45I	GTSAC	1 cut(s)	1313
TspDTI	ATGAA	5 cut(s)	278, 764, 1415, 1627, 1755
TspGWI	ACGGA	1 cut(s)	257
TspRI	CASTG	5 cut(s)	264, 450, 1618, 1726, 1909
Van91I	CCANNNNNTGG	1 cut(s)	904
Vha464I	CTTAAG	1 cut(s)	1372
VpaK11BI	GGWCC	1 cut(s)	1085
XapI	RAATTY	3 cut(s)	14, 55, 88
XceI	RCATGY	1 cut(s)	636
XspI	CTAG	2 cut(s)	138, 863
ZrmI	AGTACT	1 cut(s)	548
Zsp2I	ATGCAT	2 cut(s)	711, 918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.