Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002410

Physical Location & Seq

Genomic Coordinates Forward (+)

4081580 .. 4084846

Gene Structure 3267 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1043300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGGGGAACAACTGTGTTGGAAACAACTGTGTCGGATCAAGGAAGGATACACTCATCCATTCAGTCTCGAGATCACTCTGGTGGTCGCGATCAACGGATGGTGTTGCCAGTAGAGGAATCAATAGTACTGATGCAACGCCGCATTTGAGTAAGAATCCGTCAGAAGGTCCTCTATACGTTCAAAACAAGGCCCCGGAAGCAGTTAAGATAGCTAAGGGAGACTATAAATCAGTGCAGGCTACAAATCAAAAGGTGAAGGTTCATCCAGACAAGCCTAATGAACCGGCAAAGCCTCAGAAACACAAGGAGGAGACGTTACTTGCGAGGCAAGTAACACTTCAGAAGGCAGAGAGTAAACTAGCACCACCAACAATTCACATGGGAGAGGCTAGACCGGTACAAGTAGTAGCGAAAAAGGAAGAGACTAAGCCAGCAGCGGCGGCAAATGTGAAACCCAAGAAGCCCCCTATTGTCAAGAGGGTGGCAAGCGCAGGGCTTCAGGTCGAGTCTGTCTTGCAAACAAAAACAGGGCATTTGAAGGAGTACTACAATTTGGGCCAAAAGCTCGGACATGGGCAGTTTGGGACAACGTTTCTGTGTGTAGAGAAAGCAACCGGAAAGGAGTATGCTTGTAAATCCATTGCAAAAAGGAAACTCCAGACTACAGAAGATGTTGAAGATGTGAGGAGAGAAATTCAGATTATGCATCACTTAGCAGGGAATCAAAATGTAATTGCGATTAAATCAGCGTTTGAGGATGCTGTTGCGGTTCATGTTGTCATGGAATTATGTGCAGGGGGTGAGCTTTTCGATAGGATTATCAAGCGAGGGCATTACACCGAAAGGAAGGCAGCTCAACTGACAAGGACTATAGTTGGAGTTATAGAAGCCTGCCATTCTTTGGGTGTCATGCATCGTGATCTTAAGCCGGAGAACTTTCTTTTTGTCAACGAGGAGGAGGATTCATCACTTAAGGCTATCGATTTTGGACTATCAATATTCTTCAAGCCAGGGGATATATTCGGTGATGTGGTTGGAAGCCCCTATTATGTTGCACCAGAAGTCCTGCGCAAGCGTTATGGTCCAGAAGCAGATGTTTGGAGTGCTGGTGTTATCATTTACATTCTCTTAAGTGGAGTGCCTCCATTTTGGGGTGAAACTGAGCAAGAGATATTTGAAGAGGTGCTGCATGGCGATCTTGATTTCTCATCGGATCCCTGGCCTAGTATTTCTGAAAGTGCCAAAGATCTCGTTCAGAGAATGCTCGTTAGAAACCCAAAAAAGCGGCTAACTGCTCATCAAGTTCTGTGCCACCCATGGGTTCAGGTTGATGGAGTGGCTCCAGACAAGCCTCTTGATTCCGCTGTTTTAAGTCGCTTGAAGCAGTTTTCTGCAATGAACAAAATTAAGAAAATGGCTCTAAGAGTCATTGCAGAGAACCTCTCGGAAGAAGAAATTGCTGGTTTGAAAGAAATGTTCAAGATGATAGATACCGACAATAGCGGTCAAATTAGTTTTGATGAACTCAGAGATGGACTCCAAAGATTTGGAGCTAATCTAAATGAGTCTGAAATATATGATCTTATGAAAGCTGCAGATGTTGACAATAGTGGCACAATTGACTACGGAGAGTTTATAGCTGCAACATTGCATCTAAACAAAATAGAGAAAGAAGACCATTTATTTGCAGCTTTCTCATATTTTGACAAGGACGGCAGTGGCTACATCACACAAGATGAACTTGAACAAGCCTGCGAGGAGTTTGGCATTGTCGATGTTCACTTGGAAGAACTGATCCGAGAAGTCGATCAGGACAATGACGGGCGAATAGACTACAACGAGTTTGTCGCCATGATGAAGAAAGGCAATGCAGATTTTGATAAGAAGGGGCTTCACACCAGTGCCCTTGGCGTTGGATTTAGGGAGGCACTATCTGTTTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031984 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0098827 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

648

Amino Acids

71.84

Weight (kDa)

5.91

Isoelectric Point (pI)

38.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	183 - 441	1.2e-75	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	185 - 435	5.6e-23	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	489 - 515	2.9e-08	EF hand domain
EF-hand_7	PF13499	489 - 548	2.6e-13	EF-hand domain pair
EF-hand_6	PF13405	489 - 517	4.1e-07	EF-hand domain
EF-hand_5	PF13202	489 - 511	7.3e-06	EF hand
EF-hand_8	PF13833	502 - 549	3.4e-08	EF-hand domain pair
EF-hand_7	PF13499	558 - 620	8.6e-16	EF-hand domain pair
EF-hand_1	PF00036	562 - 586	1e-05	EF hand domain
EF-hand_6	PF13405	562 - 589	1.6e-06	EF-hand domain
EF-hand_1	PF00036	595 - 621	2e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10660 AT3G10660 AT5G04870
fragaria_vesca	FvH4_6g20840 FvH4_6g20840 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490
malus_domestica	MD02G1257300.v1.1 MD07G1063300.v1.1 MD12G1044400.v1.1 MD14G1043300.v1.1
prunus_persica	Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.7G090800_v2.0.a1 Prupe.7G090800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0332341 RchiOBHm_Chr3g0464211 RchiOBHm_Chr3g0477181
rosa_laevigata	RLG00000023739 RLG00000029678
rosa_multiflora	Rmu_sc0000932.1_g000001 Rmu_sc0003422.1_g000006
rosa_roxburghii	Rroxscaffold_4G00318280 Rroxscaffold_6G00404350 Rroxscaffold_6G00404850
rosa_rugosa	Rorug01G0100000 Rorug03G0158600
rosa_samantha	Rh1CG118000 Rh1DG129100 Rh3AG208900 Rh3CG236600 Rh3DG235400
rosa_wichuraiana	Rw1G010080 Rw3G019050 Rw3G019440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1070
AccB7I	CCANNNNNTGG	1 cut(s)	901
AccII	CGCG	1 cut(s)	88
AciI	CCGC	7 cut(s)	140, 437, 440, 767, 1285, 1362, 1503
AclI	AACGTT	1 cut(s)	590
AclWI	GGATC	4 cut(s)	43, 1208, 1221, 1789
AcsI	RAATTY	1 cut(s)	693
AcuI	CTGAAG	2 cut(s)	323, 482
AfaI	GTAC	3 cut(s)	127, 399, 545
AfiI	CCNNNNNNNGG	4 cut(s)	164, 901, 1151, 1318
AflII	CTTAAG	3 cut(s)	923, 971, 1129
AgeI	ACCGGT	1 cut(s)	394
AgsI	TTSAA	9 cut(s)	182, 538, 677, 1006, 1178, 1381, 1468, 1480, 1745
AjnI	CCWGG	2 cut(s)	1009, 1217
AleI	CACNNNNGTG	2 cut(s)	79, 1899
AluBI	AGCT	8 cut(s)	212, 565, 805, 854, 1553, 1592, 1640, 1691
AluI	AGCT	8 cut(s)	212, 565, 805, 854, 1553, 1592, 1640, 1691
Alw26I	GTCTC	4 cut(s)	70, 213, 305, 416
AlwI	GGATC	4 cut(s)	43, 1208, 1221, 1789
Ama87I	CYCGRG	1 cut(s)	67
AoxI	GGCC	3 cut(s)	189, 556, 1220
ApeKI	GCWGC	6 cut(s)	434, 851, 1186, 1592, 1640, 1688
ApoI	RAATTY	1 cut(s)	693
ArsI	GACNNNNNNTTYG	2 cut(s)	1667, 1699
AsiGI	ACCGGT	1 cut(s)	394
AspLEI	GCGC	2 cut(s)	491, 1071
AspS9I	GGNCC	4 cut(s)	167, 190, 556, 1082
AsuC2I	CCSGG	1 cut(s)	194
AsuHPI	GGTGA	4 cut(s)	265, 812, 1037, 1166
AvaI	CYCGRG	1 cut(s)	67
AvaII	GGWCC	2 cut(s)	167, 1082
BaeGI	GKGCMC	1 cut(s)	1906
BamHI	GGATCC	1 cut(s)	1213
BarI	GAAGNNNNNNTAC	2 cut(s)	530, 562
BbsI	GAAGAC	1 cut(s)	1680
BbvI	GCAGC	6 cut(s)	446, 863, 1173, 1579, 1627, 1700
BccI	CCATC	3 cut(s)	92, 1325, 1526
BceAI	ACGGC	1 cut(s)	1729
BciT130I	CCWGG	2 cut(s)	1011, 1219
BciVI	GTATCC	1 cut(s)	40
BcnI	CCSGG	1 cut(s)	194
BcoDI	GTCTC	4 cut(s)	70, 213, 305, 416
BfaI	CTAG	3 cut(s)	359, 390, 1224
BfmI	CTRYAG	3 cut(s)	663, 870, 1593
BfrI	CTTAAG	3 cut(s)	923, 971, 1129
BfuI	GTATCC	1 cut(s)	40
BglII	AGATCT	1 cut(s)	1246
BmcAI	AGTACT	2 cut(s)	127, 545
Bme1390I	CCNGG	3 cut(s)	194, 1011, 1219
Bme18I	GGWCC	2 cut(s)	167, 1082
BmeT110I	CYCGRG	1 cut(s)	67
BmgT120I	GGNCC	4 cut(s)	167, 190, 556, 1082
BmiI	GGNNCC	3 cut(s)	192, 1215, 1341
BmrFI	CCNGG	3 cut(s)	194, 1011, 1219
BmsI	GCATC	5 cut(s)	121, 715, 748, 922, 1660
BpiI	GAAGAC	1 cut(s)	1680
BplI	GAGNNNNNCTC	4 cut(s)	1427, 1459, 1521, 1553
BpmI	CTGGAG	2 cut(s)	641, 1326
Bpu10I	CCTNAGC	1 cut(s)	213
BpuMI	CCSGG	1 cut(s)	194
Bsa29I	ATCGAT	1 cut(s)	981
BsaJI	CCNNGG	5 cut(s)	192, 1010, 1217, 1316, 1906
BsaWI	WCCGGW	2 cut(s)	394, 614
BsaXI	ACNNNNNCTCC	4 cut(s)	299, 329, 1093, 1123
Bsc4I	CCNNNNNNNGG	4 cut(s)	164, 901, 1151, 1318
Bse118I	RCCGGY	2 cut(s)	283, 394
Bse1I	ACTGG	2 cut(s)	108, 1899
Bse3DI	GCAATG	5 cut(s)	639, 1401, 1428, 1646, 1873
BseBI	CCWGG	2 cut(s)	1011, 1219
BseCI	ATCGAT	1 cut(s)	981
BseDI	CCNNGG	5 cut(s)	192, 1010, 1217, 1316, 1906
BseGI	GGATG	4 cut(s)	54, 103, 262, 763
BseLI	CCNNNNNNNGG	4 cut(s)	164, 901, 1151, 1318
BseMI	GCAATG	5 cut(s)	639, 1401, 1428, 1646, 1873
BseMII	CTCAG	3 cut(s)	308, 1152, 1540
BseNI	ACTGG	2 cut(s)	108, 1899
BseRI	GAGGAG	5 cut(s)	323, 700, 968, 971, 1772
BseSI	GKGCMC	1 cut(s)	1906
BseXI	GCAGC	6 cut(s)	446, 863, 1173, 1579, 1627, 1700
BsgI	GTGCAG	2 cut(s)	254, 813
Bsh1236I	CGCG	1 cut(s)	88
BshFI	GGCC	3 cut(s)	191, 558, 1222
BshTI	ACCGGT	1 cut(s)	394
BshVI	ATCGAT	1 cut(s)	981
BsiHKCI	CYCGRG	1 cut(s)	67
BsiSI	CCGG	5 cut(s)	194, 284, 395, 615, 929
BslFI	GGGAC	1 cut(s)	598
BslI	CCNNNNNNNGG	4 cut(s)	164, 901, 1151, 1318
BsmAI	GTCTC	4 cut(s)	70, 213, 305, 416
BsmBI	CGTCTC	1 cut(s)	305
BsmFI	GGGAC	1 cut(s)	598
BsmI	GAATGC	1 cut(s)	1266
BsnI	GGCC	3 cut(s)	191, 558, 1222
BsoBI	CYCGRG	1 cut(s)	67
Bsp1286I	GDGCHC	1 cut(s)	1906
Bsp19I	CCATGG	1 cut(s)	1316
Bsp68I	TCGCGA	1 cut(s)	88
BspACI	CCGC	7 cut(s)	140, 437, 440, 767, 1285, 1362, 1503
BspANI	GGCC	3 cut(s)	191, 558, 1222
BspCNI	CTCAG	3 cut(s)	307, 1153, 1539
BspDI	ATCGAT	1 cut(s)	981
BspFNI	CGCG	1 cut(s)	88
BspLI	GGNNCC	3 cut(s)	192, 1215, 1341
BspMAI	CTGCAG	1 cut(s)	1597
BspPI	GGATC	4 cut(s)	43, 1208, 1221, 1789
BspTI	CTTAAG	3 cut(s)	923, 971, 1129
BsrDI	GCAATG	5 cut(s)	639, 1401, 1428, 1646, 1873
BsrFI	RCCGGY	2 cut(s)	283, 394
BsrI	ACTGG	2 cut(s)	108, 1899
BssAI	RCCGGY	2 cut(s)	283, 394
BssECI	CCNNGG	5 cut(s)	192, 1010, 1217, 1316, 1906
BssT1I	CCWWGG	2 cut(s)	1316, 1906
Bst2UI	CCWGG	2 cut(s)	1011, 1219
Bst4CI	ACNGT	2 cut(s)	14, 29
Bst6I	CTCTTC	2 cut(s)	414, 1173
BstAFI	CTTAAG	3 cut(s)	923, 971, 1129
BstC8I	GCNNGC	6 cut(s)	237, 432, 487, 892, 1073, 1753
BstDEI	CTNAG	7 cut(s)	213, 294, 426, 712, 1161, 1421, 1526
BstDSI	CCRYGG	1 cut(s)	1316
BstF5I	GGATG	4 cut(s)	54, 103, 262, 763
BstFNI	CGCG	1 cut(s)	88
BstHHI	GCGC	2 cut(s)	491, 1071
BstMAI	GTCTC	4 cut(s)	70, 213, 305, 416
BstMWI	GCNNNNNNNGC	2 cut(s)	197, 440
BstNI	CCWGG	2 cut(s)	1011, 1219
BstSCI	CCNGG	3 cut(s)	192, 1009, 1217
BstSFI	CTRYAG	3 cut(s)	663, 870, 1593
BstSLI	GKGCMC	1 cut(s)	1906
BstUI	CGCG	1 cut(s)	88
BstV1I	GCAGC	6 cut(s)	446, 863, 1173, 1579, 1627, 1700
BstV2I	GAAGAC	1 cut(s)	1680
BstX2I	RGATCY	2 cut(s)	1213, 1246
BstXI	CCANNNNNNTGG	1 cut(s)	1547
BstYI	RGATCY	2 cut(s)	1213, 1246
Bsu15I	ATCGAT	1 cut(s)	981
BsuI	GTATCC	1 cut(s)	40
BsuRI	GGCC	3 cut(s)	191, 558, 1222
BsuTUI	ATCGAT	1 cut(s)	981
BtgI	CCRYGG	1 cut(s)	1316
BtsCI	GGATG	4 cut(s)	54, 103, 262, 763
BtsI	GCAGTG	1 cut(s)	1723
BtsIMutI	CAGTG	3 cut(s)	237, 1723, 1906
BtuMI	TCGCGA	1 cut(s)	88
Cac8I	GCNNGC	6 cut(s)	237, 432, 487, 892, 1073, 1753
CfoI	GCGC	2 cut(s)	491, 1071
Cfr10I	RCCGGY	2 cut(s)	283, 394
Cfr13I	GGNCC	4 cut(s)	167, 190, 556, 1082
ClaI	ATCGAT	1 cut(s)	981
Csp6I	GTAC	3 cut(s)	126, 398, 544
CspAI	ACCGGT	1 cut(s)	394
CviAII	CATG	8 cut(s)	379, 572, 773, 781, 910, 1190, 1317, 1852
CviQI	GTAC	3 cut(s)	126, 398, 544
DdeI	CTNAG	7 cut(s)	213, 294, 426, 712, 1161, 1421, 1526
Eam1104I	CTCTTC	2 cut(s)	414, 1173
EarI	CTCTTC	2 cut(s)	414, 1173
Eco130I	CCWWGG	2 cut(s)	1316, 1906
Eco47I	GGWCC	2 cut(s)	167, 1082
Eco57I	CTGAAG	2 cut(s)	323, 482
Eco88I	CYCGRG	1 cut(s)	67
EcoO109I	RGGNCCY	2 cut(s)	167, 190
EcoRII	CCWGG	2 cut(s)	1009, 1217
EcoT14I	CCWWGG	2 cut(s)	1316, 1906
EcoT22I	ATGCAT	2 cut(s)	708, 915
ErhI	CCWWGG	2 cut(s)	1316, 1906
Esp3I	CGTCTC	1 cut(s)	305
FaeI	CATG	8 cut(s)	382, 575, 776, 784, 913, 1193, 1320, 1855
FalI	AAGNNNNNCTT	4 cut(s)	321, 353, 1725, 1757
FaqI	GGGAC	1 cut(s)	598
FatI	CATG	8 cut(s)	378, 571, 772, 780, 909, 1189, 1316, 1851
FokI	GGATG	4 cut(s)	41, 110, 249, 770
FspBI	CTAG	3 cut(s)	359, 390, 1224
FspI	TGCGCA	1 cut(s)	1070
GlaI	GCGC	2 cut(s)	490, 1070
GsuI	CTGGAG	2 cut(s)	641, 1326
HaeIII	GGCC	3 cut(s)	191, 558, 1222
HapII	CCGG	5 cut(s)	194, 284, 395, 615, 929
HhaI	GCGC	2 cut(s)	491, 1071
Hin1II	CATG	8 cut(s)	382, 575, 776, 784, 913, 1193, 1320, 1855
Hin6I	GCGC	2 cut(s)	489, 1069
HinP1I	GCGC	2 cut(s)	489, 1069
HincII	GTYRAC	2 cut(s)	949, 1603
HindII	GTYRAC	2 cut(s)	949, 1603
HinfI	GANTC	9 cut(s)	117, 154, 506, 721, 962, 1358, 1425, 1536, 1565
HpaII	CCGG	5 cut(s)	194, 284, 395, 615, 929
HphI	GGTGA	4 cut(s)	265, 812, 1037, 1166
Hpy166II	GTNNAC	4 cut(s)	356, 949, 1603, 1780
Hpy8I	GTNNAC	4 cut(s)	356, 949, 1603, 1780
HpyAV	CCTTC	7 cut(s)	37, 158, 250, 337, 532, 841, 1879
HpyCH4III	ACNGT	2 cut(s)	14, 29
HpyCH4IV	ACGT	3 cut(s)	177, 314, 590
HpyF10VI	GCNNNNNNNGC	2 cut(s)	197, 440
HpyF3I	CTNAG	7 cut(s)	213, 294, 426, 712, 1161, 1421, 1526
HpySE526I	ACGT	3 cut(s)	177, 314, 590
Hsp92II	CATG	8 cut(s)	382, 575, 776, 784, 913, 1193, 1320, 1855
HspAI	GCGC	2 cut(s)	489, 1069
LmnI	GCTCC	2 cut(s)	1345, 1550
Lsp1109I	GCAGC	6 cut(s)	446, 863, 1173, 1579, 1627, 1700
LweI	GCATC	5 cut(s)	121, 715, 748, 922, 1660
MaeI	CTAG	3 cut(s)	359, 390, 1224
MaeII	ACGT	3 cut(s)	177, 314, 590
MaeIII	GTNAC	2 cut(s)	315, 331
MfeI	CAATTG	1 cut(s)	1617
MflI	RGATCY	2 cut(s)	1213, 1246
MhlI	GDGCHC	1 cut(s)	1906
MluCI	AATT	9 cut(s)	372, 550, 693, 732, 785, 1404, 1455, 1509, 1617
MlyI	GAGTC	4 cut(s)	515, 1434, 1530, 1574
MmeI	TCCRAC	4 cut(s)	13, 856, 1015, 1894
Mph1103I	ATGCAT	2 cut(s)	708, 915
MseI	TTAA	8 cut(s)	204, 741, 924, 972, 1130, 1370, 1407, 1942
MslI	CAYNNNNRTG	2 cut(s)	79, 1899
MspA1I	CMGCKG	2 cut(s)	437, 1364
MspCI	CTTAAG	3 cut(s)	923, 971, 1129
MspI	CCGG	5 cut(s)	194, 284, 395, 615, 929
MspR9I	CCNGG	3 cut(s)	194, 1011, 1219
MunI	CAATTG	1 cut(s)	1617
Mva1269I	GAATGC	1 cut(s)	1266
MvaI	CCWGG	2 cut(s)	1011, 1219
MvnI	CGCG	1 cut(s)	88
MwoI	GCNNNNNNNGC	2 cut(s)	197, 440
NciI	CCSGG	1 cut(s)	194
NcoI	CCATGG	1 cut(s)	1316
NlaIII	CATG	8 cut(s)	382, 575, 776, 784, 913, 1193, 1320, 1855
NlaIV	GGNNCC	3 cut(s)	192, 1215, 1341
NruI	TCGCGA	1 cut(s)	88
NsbI	TGCGCA	1 cut(s)	1070
NsiI	ATGCAT	2 cut(s)	708, 915
OliI	CACNNNNGTG	2 cut(s)	79, 1899
PaeR7I	CTCGAG	1 cut(s)	67
PcsI	WCGNNNNNNNCGW	1 cut(s)	320
PctI	GAATGC	1 cut(s)	1266
PfeI	GAWTC	5 cut(s)	117, 154, 721, 962, 1358
PflMI	CCANNNNNTGG	1 cut(s)	901
PinAI	ACCGGT	1 cut(s)	394
PleI	GAGTC	4 cut(s)	514, 1433, 1530, 1573
PpsI	GAGTC	4 cut(s)	514, 1433, 1530, 1573
PpuMI	RGGWCCY	1 cut(s)	167
Psp1406I	AACGTT	1 cut(s)	590
Psp5II	RGGWCCY	1 cut(s)	167
Psp6I	CCWGG	2 cut(s)	1009, 1217
PspGI	CCWGG	2 cut(s)	1009, 1217
PspN4I	GGNNCC	3 cut(s)	192, 1215, 1341
PspPI	GGNCC	4 cut(s)	167, 190, 556, 1082
PspPPI	RGGWCCY	1 cut(s)	167
PstI	CTGCAG	1 cut(s)	1597
PsuI	RGATCY	2 cut(s)	1213, 1246
RruI	TCGCGA	1 cut(s)	88
RsaI	GTAC	3 cut(s)	127, 399, 545
RsaNI	GTAC	3 cut(s)	126, 398, 544
RseI	CAYNNNNRTG	2 cut(s)	79, 1899
SaqAI	TTAA	8 cut(s)	204, 741, 924, 972, 1130, 1370, 1407, 1942
Sau96I	GGNCC	4 cut(s)	167, 190, 556, 1082
ScaI	AGTACT	2 cut(s)	127, 545
SchI	GAGTC	4 cut(s)	515, 1434, 1530, 1574
ScrFI	CCNGG	3 cut(s)	194, 1011, 1219
SduI	GDGCHC	1 cut(s)	1906
SfaNI	GCATC	5 cut(s)	121, 715, 748, 922, 1660
SfcI	CTRYAG	3 cut(s)	663, 870, 1593
Sfr274I	CTCGAG	1 cut(s)	67
SinI	GGWCC	2 cut(s)	167, 1082
SlaI	CTCGAG	1 cut(s)	67
SmiMI	CAYNNNNRTG	2 cut(s)	79, 1899
SmlI	CTYRAG	4 cut(s)	67, 923, 971, 1129
SmoI	CTYRAG	4 cut(s)	67, 923, 971, 1129
Sse9I	AATT	9 cut(s)	372, 550, 693, 732, 785, 1404, 1455, 1509, 1617
SsiI	CCGC	7 cut(s)	140, 437, 440, 767, 1285, 1362, 1503
SspI	AATATT	1 cut(s)	999
SspMI	CTAG	3 cut(s)	359, 390, 1224
StyD4I	CCNGG	3 cut(s)	192, 1009, 1217
StyI	CCWWGG	2 cut(s)	1316, 1906
TaaI	ACNGT	2 cut(s)	14, 29
TaiI	ACGT	3 cut(s)	180, 317, 593
TaqI	TCGA	6 cut(s)	68, 504, 810, 981, 1773, 1806
TasI	AATT	9 cut(s)	372, 550, 693, 732, 785, 1404, 1455, 1509, 1617
TatI	WGTACW	2 cut(s)	125, 543
TauI	GCSGC	4 cut(s)	142, 440, 443, 1288
TfiI	GAWTC	5 cut(s)	117, 154, 721, 962, 1358
Tru1I	TTAA	8 cut(s)	204, 741, 924, 972, 1130, 1370, 1407, 1942
Tru9I	TTAA	8 cut(s)	204, 741, 924, 972, 1130, 1370, 1407, 1942
TscAI	CASTG	3 cut(s)	237, 1723, 1906
TseI	GCWGC	6 cut(s)	434, 851, 1186, 1592, 1640, 1688
TspDTI	ATGAA	9 cut(s)	251, 294, 761, 954, 1412, 1536, 1601, 1752, 1871
TspGWI	ACGGA	3 cut(s)	110, 147, 1641
TspRI	CASTG	3 cut(s)	237, 1723, 1906
Van91I	CCANNNNNTGG	1 cut(s)	901
Vha464I	CTTAAG	3 cut(s)	923, 971, 1129
VpaK11BI	GGWCC	2 cut(s)	167, 1082
XapI	RAATTY	1 cut(s)	693
XhoI	CTCGAG	1 cut(s)	67
XspI	CTAG	3 cut(s)	359, 390, 1224
ZrmI	AGTACT	2 cut(s)	127, 545
Zsp2I	ATGCAT	2 cut(s)	708, 915

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.