Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002410

Physical Location & Seq

Genomic Coordinates Reverse (-)

5928767 .. 5933298

Gene Structure 4532 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1063300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGGGTAACACTTGTGTTGGACCGAGCATTTCCAAGAATGCTTTCTTCCAATCAGTTTCAGCTGTAATGTGGCCAAACCGCTCTCCCGATGACTCAGTTCATACTAACAGAGAACCTGTCAATGAGACATCATCACCTAAGGAACCTGAGTCGCCTATGCCAGTACTAGACAAACCACCAGAGCAAGTGACAATACCAAAACCAGAAACCGAGCCAAAGCAATCCGCAAAACCTGAAACTGAGCCAAAACAATCCGCAAAACCCAAGAAGCCACCGCAGATAAAGAGGGTCCCTAGTGCAGGACTTCAGGCTGGTTCGGTGTTGCAAACGAAAACCGGAAAGATAAAGGAGTTTTACAGTTTTGGGAGGAAACTTGGACAGGGGCAGTTTGGAATAACGTTTCTGTGTGTGGAGAAGGCGACAGGGAAAGAGTATGCGTGCAAATCGATTGCGAAAAGGAAGTTAATAACTGATGACGATGTGGAGGATGTGAGGAGGGAAATTCAGATCATGCACCATCTGGCAGGCCACCCAAATGTTATCTCTATTAAGGGAGCTTATGAGGATGCTGTAGCAGTTCATGTTGTTATGGAATTATGTGCAGGAGGTGAGCTCTTTGATAGGATTATCCAGCGCGGGCATTATACAGAAAGAAAGGCAGCTGAGCTAACTAGGACTATAGTTGGAGTGGTCGAAGCTTGCCATTCATTGGGAGTTATGCATCGAGACCTTAAACCAGAGAACTTTCTCTTTGTCAGTCAGGATGAGGATGCACTTCTCAAAACTATTGATTTTGGATTGTCAATTTTCTTGAAGCCAGGAGTAAAATATAGTGATGTGGTTGGAAGCCCATATTATGTTGCACCTGAAGTGTTACGCAAGCGTTATGGTCCAGAAGCAGATGTCTGGAGTGCAGGGGTGATCGTTTACATTCTGTTAAGTGGGGTACCGCCATTTTGGGCTGAAAATGAGGAAGGAATATTTGACCAGGTCCTACATGGTGACCTAGATTTCGAATCAGACCCTTGGCCTAGTATTTCTGATGGTGCCAAAGATTTAGTGAGGAGAATGCTTGTCCGAGACCCCAAAAGGCGGCTGACAGCCCATGAAGTTTTGTGCCACCCCTGGGTGCAAGTTGATGGTGTGGCTCCCGATAAGGCTCTTGATTCTGCAGTTCTAAGTCGCTTAAAACAATTTTCGGCTATGAACAAACTCAAGAAAATGGCTCTCAGAGTCATTGCAGAGAACTTGTCTGTAGAAGAAATAGCTGGCCTGAAAGAAATGTTCAAGATGATAGACACTGACAACAGTGGTCAAATCACTTTTGACGAACTAAAGATTGGATTGAAAAGATTTGGGGCAAATCTAAAGGAGTCTGAAATTTATGATCTAATGCAAGCAGTATGTACCTCTTACCCTCTCTGGCCAGTGCTTCGTCAAGCCATAGCTACTATGGTCATGCCAGCCATGTTGACCTGTTTTAAGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031090 GO:0031903 GO:0031984 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042175 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0098588 GO:0098805 GO:0098827 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

497

Amino Acids

55.19

Weight (kDa)

6.62

Isoelectric Point (pI)

48.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	119 - 377	8.7e-77	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	121 - 371	5.6e-24	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	425 - 451	4.2e-08	EF hand domain
EF-hand_6	PF13405	425 - 453	9.1e-07	EF-hand domain
EF-hand_7	PF13499	425 - 466	4e-06	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10660 AT3G10660 AT5G04870
fragaria_vesca	FvH4_6g20840 FvH4_6g20840 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490
malus_domestica	MD02G1257300.v1.1 MD07G1063300.v1.1 MD12G1044400.v1.1 MD14G1043300.v1.1
prunus_persica	Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.7G090800_v2.0.a1 Prupe.7G090800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0332341 RchiOBHm_Chr3g0464211 RchiOBHm_Chr3g0477181
rosa_laevigata	RLG00000023739 RLG00000029678
rosa_multiflora	Rmu_sc0000932.1_g000001 Rmu_sc0003422.1_g000006
rosa_roxburghii	Rroxscaffold_4G00318280 Rroxscaffold_6G00404350 Rroxscaffold_6G00404850
rosa_rugosa	Rorug01G0100000 Rorug03G0158600
rosa_samantha	Rh1CG118000 Rh1DG129100 Rh3AG208900 Rh3CG236600 Rh3DG235400
rosa_wichuraiana	Rw1G010080 Rw3G019050 Rw3G019440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	946
AccB1I	GGYRCC	2 cut(s)	946, 1046
AccB7I	CCANNNNNTGG	1 cut(s)	709
AccBSI	CCGCTC	1 cut(s)	81
AccII	CGCG	1 cut(s)	636
AciI	CCGC	7 cut(s)	79, 225, 255, 275, 636, 950, 1093
AclI	AACGTT	1 cut(s)	398
AcoI	YGGCCR	2 cut(s)	71, 1424
AcsI	RAATTY	2 cut(s)	501, 1380
AcuI	CTGAAG	2 cut(s)	290, 888
AfaI	GTAC	3 cut(s)	165, 948, 1408
AfiI	CCNNNNNNNGG	4 cut(s)	299, 709, 1092, 1126
AgsI	TTSAA	3 cut(s)	814, 1288, 1348
AjnI	CCWGG	3 cut(s)	817, 987, 1124
AjuI	GAANNNNNNNTTGG	4 cut(s)	26, 58, 1323, 1355
AluBI	AGCT	8 cut(s)	62, 557, 613, 662, 667, 698, 1268, 1448
AluI	AGCT	8 cut(s)	62, 557, 613, 662, 667, 698, 1268, 1448
Alw21I	GWGCWC	1 cut(s)	615
Alw26I	GTCTC	3 cut(s)	119, 720, 1074
AoxI	GGCC	5 cut(s)	71, 526, 1028, 1270, 1424
ApeKI	GCWGC	1 cut(s)	659
ApoI	RAATTY	2 cut(s)	501, 1380
ArsI	GACNNNNNNTTYG	2 cut(s)	995, 1027
Asp700I	GAANNNNTTC	1 cut(s)	41
Asp718I	GGTACC	1 cut(s)	946
AspLEI	GCGC	1 cut(s)	636
AspS9I	GGNCC	4 cut(s)	20, 289, 890, 991
AsuHPI	GGTGA	4 cut(s)	126, 620, 931, 1013
AsuII	TTCGAA	1 cut(s)	1014
AvaII	GGWCC	4 cut(s)	20, 289, 890, 991
AxyI	CCTNAGG	1 cut(s)	138
BalI	TGGCCA	2 cut(s)	73, 1426
BanI	GGYRCC	2 cut(s)	946, 1046
BanII	GRGCYC	1 cut(s)	615
Bbv12I	GWGCWC	1 cut(s)	615
BbvI	GCAGC	1 cut(s)	671
BccI	CCATC	3 cut(s)	525, 1037, 1133
BciT130I	CCWGG	3 cut(s)	819, 989, 1126
BcoDI	GTCTC	3 cut(s)	119, 720, 1074
BfaI	CTAG	5 cut(s)	167, 294, 672, 1007, 1032
BfmI	CTRYAG	4 cut(s)	570, 678, 1170, 1254
BisI	GCNGC	2 cut(s)	660, 1094
BlpI	GCTNAGC	1 cut(s)	663
BlsI	GCNGC	2 cut(s)	661, 1095
BmcAI	AGTACT	1 cut(s)	165
Bme1390I	CCNGG	3 cut(s)	819, 989, 1126
Bme18I	GGWCC	4 cut(s)	20, 289, 890, 991
BmgT120I	GGNCC	4 cut(s)	20, 289, 890, 991
BmiI	GGNNCC	6 cut(s)	144, 290, 291, 948, 1048, 1149
BmrFI	CCNGG	3 cut(s)	819, 989, 1126
BmsI	GCATC	3 cut(s)	556, 730, 760
BplI	GAGNNNNNCTC	2 cut(s)	597, 629
BpmI	CTGGAG	1 cut(s)	928
Bpu1102I	GCTNAGC	1 cut(s)	663
Bpu14I	TTCGAA	1 cut(s)	1014
BpuEI	CTTGAG	1 cut(s)	1199
Bsa29I	ATCGAT	1 cut(s)	446
BsaI	GGTCTC	2 cut(s)	720, 1074
BsaJI	CCNNGG	3 cut(s)	1025, 1124, 1125
BsaWI	WCCGGW	1 cut(s)	335
Bsc4I	CCNNNNNNNGG	4 cut(s)	299, 709, 1092, 1126
Bse1I	ACTGG	2 cut(s)	161, 1427
Bse21I	CCTNAGG	1 cut(s)	138
Bse3DI	GCAATG	1 cut(s)	1236
BseBI	CCWGG	3 cut(s)	819, 989, 1126
BseCI	ATCGAT	1 cut(s)	446
BseDI	CCNNGG	3 cut(s)	1025, 1124, 1125
BseGI	GGATG	4 cut(s)	493, 571, 769, 775
BseLI	CCNNNNNNNGG	4 cut(s)	299, 709, 1092, 1126
BseMI	GCAATG	1 cut(s)	1236
BseMII	CTCAG	5 cut(s)	108, 138, 231, 654, 1243
BseNI	ACTGG	2 cut(s)	161, 1427
BseRI	GAGGAG	2 cut(s)	508, 1078
BseXI	GCAGC	1 cut(s)	671
BsgI	GTGCAG	3 cut(s)	318, 621, 933
Bsh1236I	CGCG	1 cut(s)	636
BshFI	GGCC	5 cut(s)	73, 528, 1030, 1272, 1426
BshNI	GGYRCC	2 cut(s)	946, 1046
BshVI	ATCGAT	1 cut(s)	446
BsiHKAI	GWGCWC	1 cut(s)	615
BsiSI	CCGG	1 cut(s)	336
BslFI	GGGAC	1 cut(s)	275
BslI	CCNNNNNNNGG	4 cut(s)	299, 709, 1092, 1126
BsmAI	GTCTC	3 cut(s)	119, 720, 1074
BsmFI	GGGAC	1 cut(s)	275
BsmI	GAATGC	2 cut(s)	43, 1074
BsnI	GGCC	5 cut(s)	73, 528, 1030, 1272, 1426
Bso31I	GGTCTC	2 cut(s)	720, 1074
Bsp119I	TTCGAA	1 cut(s)	1014
Bsp1286I	GDGCHC	1 cut(s)	615
Bsp143I	GATC	3 cut(s)	507, 921, 1387
Bsp1720I	GCTNAGC	1 cut(s)	663
BspACI	CCGC	7 cut(s)	79, 225, 255, 275, 636, 950, 1093
BspANI	GGCC	5 cut(s)	73, 528, 1030, 1272, 1426
BspCNI	CTCAG	5 cut(s)	107, 139, 232, 655, 1242
BspDI	ATCGAT	1 cut(s)	446
BspFNI	CGCG	1 cut(s)	636
BspLI	GGNNCC	6 cut(s)	144, 290, 291, 948, 1048, 1149
BspMAI	CTGCAG	1 cut(s)	1174
BspT104I	TTCGAA	1 cut(s)	1014
BspT107I	GGYRCC	2 cut(s)	946, 1046
BspTNI	GGTCTC	2 cut(s)	720, 1074
BsrBI	CCGCTC	1 cut(s)	81
BsrDI	GCAATG	1 cut(s)	1236
BsrI	ACTGG	2 cut(s)	161, 1427
BssECI	CCNNGG	3 cut(s)	1025, 1124, 1125
BssMI	GATC	3 cut(s)	507, 921, 1387
BssT1I	CCWWGG	1 cut(s)	1025
Bst2UI	CCWGG	3 cut(s)	819, 989, 1126
Bst4CI	ACNGT	2 cut(s)	359, 1310
BstBI	TTCGAA	1 cut(s)	1014
BstC8I	GCNNGC	8 cut(s)	439, 526, 638, 700, 881, 1270, 1398, 1464
BstDEI	CTNAG	7 cut(s)	94, 138, 147, 240, 663, 1178, 1229
BstEII	GGTNACC	1 cut(s)	1001
BstENI	CCTNNNNNAGG	1 cut(s)	297
BstF5I	GGATG	4 cut(s)	493, 571, 769, 775
BstFNI	CGCG	1 cut(s)	636
BstHHI	GCGC	1 cut(s)	636
BstKTI	GATC	3 cut(s)	510, 924, 1390
BstMAI	GTCTC	3 cut(s)	119, 720, 1074
BstMBI	GATC	3 cut(s)	507, 921, 1387
BstNI	CCWGG	3 cut(s)	819, 989, 1126
BstPI	GGTNACC	1 cut(s)	1001
BstSCI	CCNGG	3 cut(s)	817, 987, 1124
BstSFI	CTRYAG	4 cut(s)	570, 678, 1170, 1254
BstUI	CGCG	1 cut(s)	636
BstV1I	GCAGC	1 cut(s)	671
Bsu15I	ATCGAT	1 cut(s)	446
Bsu36I	CCTNAGG	1 cut(s)	138
BsuRI	GGCC	5 cut(s)	73, 528, 1030, 1272, 1426
BsuTUI	ATCGAT	1 cut(s)	446
BtsCI	GGATG	4 cut(s)	493, 571, 769, 775
BtsIMutI	CAGTG	3 cut(s)	1299, 1315, 1434
Cac8I	GCNNGC	8 cut(s)	439, 526, 638, 700, 881, 1270, 1398, 1464
CfoI	GCGC	1 cut(s)	636
Cfr13I	GGNCC	4 cut(s)	20, 289, 890, 991
ClaI	ATCGAT	1 cut(s)	446
CsiI	ACCWGGT	1 cut(s)	987
Csp6I	GTAC	3 cut(s)	164, 947, 1407
CviAII	CATG	6 cut(s)	511, 581, 998, 1106, 1459, 1468
CviQI	GTAC	3 cut(s)	164, 947, 1407
DdeI	CTNAG	7 cut(s)	94, 138, 147, 240, 663, 1178, 1229
DpnI	GATC	3 cut(s)	509, 923, 1389
DpnII	GATC	3 cut(s)	507, 921, 1387
EaeI	YGGCCR	2 cut(s)	71, 1424
Ecl136II	GAGCTC	1 cut(s)	613
Eco130I	CCWWGG	1 cut(s)	1025
Eco24I	GRGCYC	1 cut(s)	615
Eco31I	GGTCTC	2 cut(s)	720, 1074
Eco47I	GGWCC	4 cut(s)	20, 289, 890, 991
Eco53kI	GAGCTC	1 cut(s)	613
Eco57I	CTGAAG	2 cut(s)	290, 888
Eco81I	CCTNAGG	1 cut(s)	138
Eco91I	GGTNACC	1 cut(s)	1001
EcoICRI	GAGCTC	1 cut(s)	613
EcoNI	CCTNNNNNAGG	1 cut(s)	297
EcoO109I	RGGNCCY	2 cut(s)	289, 991
EcoO65I	GGTNACC	1 cut(s)	1001
EcoRII	CCWGG	3 cut(s)	817, 987, 1124
EcoT14I	CCWWGG	1 cut(s)	1025
EcoT22I	ATGCAT	1 cut(s)	723
EcoT38I	GRGCYC	1 cut(s)	615
ErhI	CCWWGG	1 cut(s)	1025
FaeI	CATG	6 cut(s)	514, 584, 1001, 1109, 1462, 1471
FaqI	GGGAC	1 cut(s)	275
FatI	CATG	6 cut(s)	510, 580, 997, 1105, 1458, 1467
FauI	CCCGC	1 cut(s)	629
Fnu4HI	GCNGC	2 cut(s)	660, 1094
FokI	GGATG	4 cut(s)	500, 578, 776, 782
FriOI	GRGCYC	1 cut(s)	615
Fsp4HI	GCNGC	2 cut(s)	660, 1094
FspBI	CTAG	5 cut(s)	167, 294, 672, 1007, 1032
GlaI	GCGC	1 cut(s)	635
GluI	GCNGC	2 cut(s)	660, 1094
GsuI	CTGGAG	1 cut(s)	928
HaeIII	GGCC	5 cut(s)	73, 528, 1030, 1272, 1426
HapII	CCGG	1 cut(s)	336
HhaI	GCGC	1 cut(s)	636
Hin1II	CATG	6 cut(s)	514, 584, 1001, 1109, 1462, 1471
Hin6I	GCGC	1 cut(s)	634
HinP1I	GCGC	1 cut(s)	634
HincII	GTYRAC	1 cut(s)	1473
HindII	GTYRAC	1 cut(s)	1473
HindIII	AAGCTT	1 cut(s)	696
HinfI	GANTC	6 cut(s)	92, 149, 1016, 1166, 1233, 1373
HpaII	CCGG	1 cut(s)	336
HphI	GGTGA	4 cut(s)	126, 620, 931, 1013
Hpy166II	GTNNAC	2 cut(s)	928, 1473
Hpy188I	TCNGA	6 cut(s)	507, 1021, 1042, 1079, 1232, 1378
Hpy8I	GTNNAC	2 cut(s)	928, 1473
HpyAV	CCTTC	2 cut(s)	409, 968
HpyCH4III	ACNGT	2 cut(s)	359, 1310
HpyCH4IV	ACGT	1 cut(s)	398
HpyF3I	CTNAG	7 cut(s)	94, 138, 147, 240, 663, 1178, 1229
HpySE526I	ACGT	1 cut(s)	398
Hsp92II	CATG	6 cut(s)	514, 584, 1001, 1109, 1462, 1471
HspAI	GCGC	1 cut(s)	634
KflI	GGGWCCC	1 cut(s)	289
KpnI	GGTACC	1 cut(s)	950
Kzo9I	GATC	3 cut(s)	507, 921, 1387
LmnI	GCTCC	2 cut(s)	554, 1153
Lsp1109I	GCAGC	1 cut(s)	671
LweI	GCATC	3 cut(s)	556, 730, 760
MabI	ACCWGGT	1 cut(s)	987
MaeI	CTAG	5 cut(s)	167, 294, 672, 1007, 1032
MaeII	ACGT	1 cut(s)	398
MaeIII	GTNAC	4 cut(s)	5, 187, 873, 1001
MalI	GATC	3 cut(s)	509, 923, 1389
MbiI	CCGCTC	1 cut(s)	81
MboI	GATC	3 cut(s)	507, 921, 1387
MboII	GAAGA	2 cut(s)	37, 1271
MhlI	GDGCHC	1 cut(s)	615
MlsI	TGGCCA	2 cut(s)	73, 1426
MluCI	AATT	6 cut(s)	501, 593, 804, 1193, 1380, 1486
MluNI	TGGCCA	2 cut(s)	73, 1426
MlyI	GAGTC	4 cut(s)	86, 158, 1242, 1382
MmeI	TCCRAC	2 cut(s)	664, 823
Mox20I	TGGCCA	2 cut(s)	73, 1426
Mph1103I	ATGCAT	1 cut(s)	723
MroXI	GAANNNNTTC	1 cut(s)	41
MscI	TGGCCA	2 cut(s)	73, 1426
MseI	TTAA	7 cut(s)	464, 549, 732, 938, 1187, 1482, 1489
MslI	CAYNNNNRTG	1 cut(s)	534
Msp20I	TGGCCA	2 cut(s)	73, 1426
MspA1I	CMGCKG	2 cut(s)	62, 662
MspI	CCGG	1 cut(s)	336
MspR9I	CCNGG	3 cut(s)	819, 989, 1126
Mva1269I	GAATGC	2 cut(s)	43, 1074
MvaI	CCWGG	3 cut(s)	819, 989, 1126
MvnI	CGCG	1 cut(s)	636
NdeII	GATC	3 cut(s)	507, 921, 1387
NlaIII	CATG	6 cut(s)	514, 584, 1001, 1109, 1462, 1471
NlaIV	GGNNCC	6 cut(s)	144, 290, 291, 948, 1048, 1149
NmuCI	GTSAC	2 cut(s)	187, 1001
NsiI	ATGCAT	1 cut(s)	723
NspV	TTCGAA	1 cut(s)	1014
PasI	CCCWGGG	1 cut(s)	1125
PctI	GAATGC	2 cut(s)	43, 1074
PdmI	GAANNNNTTC	1 cut(s)	41
PfeI	GAWTC	2 cut(s)	1016, 1166
PflFI	GACNNNGTC	1 cut(s)	989
PflMI	CCANNNNNTGG	1 cut(s)	709
PkrI	GCNGC	2 cut(s)	661, 1095
PleI	GAGTC	4 cut(s)	86, 157, 1241, 1381
PpsI	GAGTC	4 cut(s)	86, 157, 1241, 1381
PpuMI	RGGWCCY	2 cut(s)	289, 991
Psp124BI	GAGCTC	1 cut(s)	615
Psp1406I	AACGTT	1 cut(s)	398
Psp5II	RGGWCCY	2 cut(s)	289, 991
Psp6I	CCWGG	3 cut(s)	817, 987, 1124
PspEI	GGTNACC	1 cut(s)	1001
PspGI	CCWGG	3 cut(s)	817, 987, 1124
PspN4I	GGNNCC	6 cut(s)	144, 290, 291, 948, 1048, 1149
PspPI	GGNCC	4 cut(s)	20, 289, 890, 991
PspPPI	RGGWCCY	2 cut(s)	289, 991
PstI	CTGCAG	1 cut(s)	1174
PsyI	GACNNNGTC	1 cut(s)	989
PvuII	CAGCTG	2 cut(s)	62, 662
RsaI	GTAC	3 cut(s)	165, 948, 1408
RsaNI	GTAC	3 cut(s)	164, 947, 1407
RseI	CAYNNNNRTG	1 cut(s)	534
SacI	GAGCTC	1 cut(s)	615
SaqAI	TTAA	7 cut(s)	464, 549, 732, 938, 1187, 1482, 1489
SatI	GCNGC	2 cut(s)	660, 1094
Sau3AI	GATC	3 cut(s)	507, 921, 1387
Sau96I	GGNCC	4 cut(s)	20, 289, 890, 991
ScaI	AGTACT	1 cut(s)	165
SchI	GAGTC	4 cut(s)	86, 158, 1242, 1382
ScrFI	CCNGG	3 cut(s)	819, 989, 1126
SduI	GDGCHC	1 cut(s)	615
SexAI	ACCWGGT	1 cut(s)	987
SfaNI	GCATC	3 cut(s)	556, 730, 760
SfcI	CTRYAG	4 cut(s)	570, 678, 1170, 1254
SfuI	TTCGAA	1 cut(s)	1014
SinI	GGWCC	4 cut(s)	20, 289, 890, 991
SmiMI	CAYNNNNRTG	1 cut(s)	534
SmlI	CTYRAG	1 cut(s)	1214
SmoI	CTYRAG	1 cut(s)	1214
Sse9I	AATT	6 cut(s)	501, 593, 804, 1193, 1380, 1486
SsiI	CCGC	7 cut(s)	79, 225, 255, 275, 636, 950, 1093
SspI	AATATT	1 cut(s)	981
SspMI	CTAG	5 cut(s)	167, 294, 672, 1007, 1032
SstI	GAGCTC	1 cut(s)	615
StyD4I	CCNGG	3 cut(s)	817, 987, 1124
StyI	CCWWGG	1 cut(s)	1025
TaaI	ACNGT	2 cut(s)	359, 1310
TaiI	ACGT	1 cut(s)	401
TaqI	TCGA	4 cut(s)	446, 693, 724, 1014
TaqII	GACCGA	1 cut(s)	37
TasI	AATT	6 cut(s)	501, 593, 804, 1193, 1380, 1486
TatI	WGTACW	1 cut(s)	163
TauI	GCSGC	1 cut(s)	1096
TfiI	GAWTC	2 cut(s)	1016, 1166
Tru1I	TTAA	7 cut(s)	464, 549, 732, 938, 1187, 1482, 1489
Tru9I	TTAA	7 cut(s)	464, 549, 732, 938, 1187, 1482, 1489
TscAI	CASTG	3 cut(s)	1306, 1315, 1434
TseFI	GTSAC	2 cut(s)	187, 1001
TseI	GCWGC	1 cut(s)	659
Tsp45I	GTSAC	2 cut(s)	187, 1001
TspDTI	ATGAA	5 cut(s)	89, 569, 696, 1122, 1220
TspRI	CASTG	3 cut(s)	1306, 1315, 1434
Tth111I	GACNNNGTC	1 cut(s)	989
Van91I	CCANNNNNTGG	1 cut(s)	709
VpaK11BI	GGWCC	4 cut(s)	20, 289, 890, 991
XagI	CCTNNNNNAGG	1 cut(s)	297
XapI	RAATTY	2 cut(s)	501, 1380
XcmI	CCANNNNNNNNNTGG	2 cut(s)	995, 1450
XmnI	GAANNNNTTC	1 cut(s)	41
XspI	CTAG	5 cut(s)	167, 294, 672, 1007, 1032
ZrmI	AGTACT	1 cut(s)	165
Zsp2I	ATGCAT	1 cut(s)	723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.