Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002410

Physical Location & Seq

Genomic Coordinates Forward (+)

30964073 .. 30971229

Gene Structure 7157 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1257300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1701 bp

ATGGGAAATACTTGTGTTGGACCGAGCATTTCCAAGAATGGTTTCTTCCAATCAGTTTCAGCTGTAATGTGGCCAAACCGGTCGCCTGATGACTCAGTTCACACAAACGGAGAAGCTGTCACTGAGACATCATCACCTAAGGAACCTGAGTCACCTATGCCAGTATTAGATAAACCACCAGAGCAAGTGACAATACCAAAACCAGAAACCGAGCCAAAACAATCCGCAAAACCCAAGACGCTACCGCAGATGAAGAGGGTCCCTAGTGCAGGACTCGCTGGTTCGGTGTTACAAACGAAAACTGGAAAGTTAAAGGAGTTTTACAGTTTGGGGAGGAAACTAGGACAAGGGCAGTTTGGAATTACGTTTCTGTGTGTGGAGAAGGCGACTGGGAAAGAGTATGCGTGCAAATCAATTGCAAAAAGGAAGTTAATAACTGATGAGGATGTGGAGGATGTGAGGAGGGAAATTCAGATCATGCACCATTTGGCAGGGCACCCAAATGTTATATCTATTAAGGGAGCTTATGAGGATGCTGTAGCAGTTCATGTTGTAATGGAATTATGTGCAGGAGGTGAGCTGTTTGATAGGATTATCCAGCGTGGGCATTATACAGAAAGAAAGGCAGCTGAGCTAACTAGGACTATAGTTGGAGTTGTCGAAGCTTGCCATTCATTGGGAGTTATGCATCGAGACCTTAAGCCGGAGAATTTTCTTTTTGTCAGTCAGGATGAGGATGCACTACTGAAAACTATTGATTTTGGATTATCTATTTTCTTGAAGCCAGGAGTAAAATTTACTGATGTGGTTGGAAGCCCATATTATGTTGCACCTGAAGTGTTACGCAAGCATTATGGTCCAGAAGCAGATGTCTGGAGTGCAGGAGTGATCCTTTACATTCTGTTAAGTGGGGTACCTCCATTTTGGGCTGAAAGTGAGCAGGGAATATTTGAACAGGTCCTGAACGGTGACTTAGATTTTGAATCAGACCCTTGGCCTAGTATTTCTGATGGTGCCAAAGATTTAGTGAGGAGAATGCTTGTCCGAGACCCTAAAAGGCGGCTGACAGCACATGAAGTTTTGTGCCACCCCTGGGTGCAAGTTGATGGTGTGGCTCCCGATAAGGCTCTTGATTCTGCAGTTCTAAGTCGCTTAAAACAATTTTCAGCTATGAACAAGCTCAAGAAAATGGCTCTCAGAGTAATTGCAGAGAGGTTGTCTGAAGAAGAAATAGCTGGCCTGAAAGAAATGTTCAAGATGATAGACACTGACAACAGTGGTCAAATTACTTTTGACGAACTCAAGGCCGGATTGAAAAGATTTGGAGCAACTCTGGAGGAGTCTGAAATTTATGATCTAATGCAAGCAGCAGATGTCGATAACAGTGGAACCATTGATTATGGGGAGTTCGTAGCTGCAACATTGCATCTGAACAAAATTGAGAGAGAAGATCATCTATTTTCAGCTTTCTCCTACTTTGATAAGGATGGAAGTGGCTATATTACTTCAGATGAGCTTCAAGTAGCTTGTGAGGAGTTTGGCATAGAGGATGTTCGCCTCGAAGAGATGATAAGAGAAGTTGATCAGGACAATGATGGACGCATAGATTACAATGAGTTTGTAGCCATGATGCAAAAAGGAAATTTTGTTGGCCCTGGTAAGAAGGGATTGCAAAGTAGCTTCAGCATTGCCTTTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031090 GO:0031903 GO:0031984 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042175 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0098588 GO:0098805 GO:0098827 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

567

Amino Acids

62.89

Weight (kDa)

5.2

Isoelectric Point (pI)

45.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	108 - 366	4.5e-77	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	110 - 360	3.5e-24	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	414 - 440	1.1e-08	EF hand domain
EF-hand_7	PF13499	414 - 473	7.3e-13	EF-hand domain pair
EF-hand_6	PF13405	414 - 442	2.4e-07	EF-hand domain
EF-hand_5	PF13202	414 - 436	8.7e-06	EF hand
EF-hand_8	PF13833	426 - 474	1.5e-07	EF-hand domain pair
EF-hand_7	PF13499	483 - 545	4.7e-15	EF-hand domain pair
EF-hand_6	PF13405	486 - 514	4.4e-06	EF-hand domain
EF-hand_1	PF00036	520 - 546	7.1e-09	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10660 AT3G10660 AT5G04870
fragaria_vesca	FvH4_6g20840 FvH4_6g20840 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490
malus_domestica	MD02G1257300.v1.1 MD07G1063300.v1.1 MD12G1044400.v1.1 MD14G1043300.v1.1
prunus_persica	Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.7G090800_v2.0.a1 Prupe.7G090800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0332341 RchiOBHm_Chr3g0464211 RchiOBHm_Chr3g0477181
rosa_laevigata	RLG00000023739 RLG00000029678
rosa_multiflora	Rmu_sc0000932.1_g000001 Rmu_sc0003422.1_g000006
rosa_roxburghii	Rroxscaffold_4G00318280 Rroxscaffold_6G00404350 Rroxscaffold_6G00404850
rosa_rugosa	Rorug01G0100000 Rorug03G0158600
rosa_samantha	Rh1CG118000 Rh1DG129100 Rh3AG208900 Rh3CG236600 Rh3DG235400
rosa_wichuraiana	Rw1G010080 Rw3G019050 Rw3G019440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	913
AccB1I	GGYRCC	3 cut(s)	495, 913, 1013
AccB7I	CCANNNNNTGG	1 cut(s)	676
AciI	CCGC	3 cut(s)	225, 245, 1060
AclWI	GGATC	1 cut(s)	883
AcoI	YGGCCR	1 cut(s)	71
AcsI	RAATTY	5 cut(s)	468, 709, 794, 1347, 1642
AcuI	CTGAAG	4 cut(s)	855, 1242, 1491, 1666
AfaI	GTAC	1 cut(s)	915
AfiI	CCNNNNNNNGG	4 cut(s)	269, 676, 703, 1093
AflII	CTTAAG	1 cut(s)	698
AgeI	ACCGGT	1 cut(s)	78
AgsI	TTSAA	6 cut(s)	781, 953, 983, 1255, 1315, 1520
AjnI	CCWGG	3 cut(s)	784, 1091, 1654
AjuI	GAANNNNNNNTTGG	2 cut(s)	26, 58
Alw26I	GTCTC	3 cut(s)	119, 687, 1041
AlwI	GGATC	1 cut(s)	883
AlwNI	CAGNNNCTG	1 cut(s)	961
AoxI	GGCC	5 cut(s)	71, 995, 1237, 1305, 1651
ApeKI	GCWGC	3 cut(s)	626, 1367, 1415
ApoI	RAATTY	5 cut(s)	468, 709, 794, 1347, 1642
ArsI	GACNNNNNNTTYG	2 cut(s)	962, 994
AsiGI	ACCGGT	1 cut(s)	78
Asp700I	GAANNNNTTC	1 cut(s)	41
Asp718I	GGTACC	1 cut(s)	913
AspS9I	GGNCC	5 cut(s)	20, 259, 857, 958, 1652
AsuHPI	GGTGA	4 cut(s)	126, 144, 587, 980
AvaII	GGWCC	4 cut(s)	20, 259, 857, 958
AxyI	CCTNAGG	1 cut(s)	138
BaeGI	GKGCMC	1 cut(s)	498
BalI	TGGCCA	1 cut(s)	73
BanI	GGYRCC	3 cut(s)	495, 913, 1013
BbvI	GCAGC	3 cut(s)	638, 1379, 1402
BccI	CCATC	4 cut(s)	1004, 1100, 1481, 1589
BciT130I	CCWGG	3 cut(s)	786, 1093, 1656
BclI	TGATCA	1 cut(s)	1582
BcoDI	GTCTC	3 cut(s)	119, 687, 1041
BfaI	CTAG	4 cut(s)	264, 341, 639, 999
BfmI	CTRYAG	3 cut(s)	537, 645, 1137
BfrI	CTTAAG	1 cut(s)	698
BisI	GCNGC	4 cut(s)	627, 1061, 1368, 1416
BlpI	GCTNAGC	1 cut(s)	630
BlsI	GCNGC	4 cut(s)	628, 1062, 1369, 1417
Bme1390I	CCNGG	3 cut(s)	786, 1093, 1656
Bme18I	GGWCC	4 cut(s)	20, 259, 857, 958
BmgT120I	GGNCC	5 cut(s)	20, 259, 857, 958, 1652
BmiI	GGNNCC	8 cut(s)	144, 260, 261, 497, 915, 1015, 1116, 1390
BmrFI	CCNGG	3 cut(s)	786, 1093, 1656
BmrI	ACTGGG	1 cut(s)	399
BmsI	GCATC	5 cut(s)	523, 697, 727, 1435, 1620
BmuI	ACTGGG	1 cut(s)	399
BpmI	CTGGAG	2 cut(s)	895, 1355
Bpu1102I	GCTNAGC	1 cut(s)	630
BpuEI	CTTGAG	2 cut(s)	1166, 1286
BsaI	GGTCTC	2 cut(s)	687, 1041
BsaJI	CCNNGG	4 cut(s)	992, 1091, 1092, 1654
BsaWI	WCCGGW	1 cut(s)	78
BsaXI	ACNNNNNCTCC	2 cut(s)	102, 132
Bsc4I	CCNNNNNNNGG	4 cut(s)	269, 676, 703, 1093
Bse118I	RCCGGY	1 cut(s)	78
Bse1I	ACTGG	3 cut(s)	161, 307, 394
Bse21I	CCTNAGG	1 cut(s)	138
Bse3DI	GCAATG	2 cut(s)	1421, 1686
BseBI	CCWGG	3 cut(s)	786, 1093, 1656
BseDI	CCNNGG	4 cut(s)	992, 1091, 1092, 1654
BseGI	GGATG	7 cut(s)	451, 460, 538, 736, 742, 1492, 1555
BseLI	CCNNNNNNNGG	4 cut(s)	269, 676, 703, 1093
BseMI	GCAATG	2 cut(s)	1421, 1686
BseMII	CTCAG	5 cut(s)	108, 114, 138, 621, 1210
BseNI	ACTGG	3 cut(s)	161, 307, 394
BseRI	GAGGAG	4 cut(s)	475, 1045, 1352, 1547
BseSI	GKGCMC	1 cut(s)	498
BseXI	GCAGC	3 cut(s)	638, 1379, 1402
BsgI	GTGCAG	3 cut(s)	288, 588, 900
Bsh1285I	CGRYCG	1 cut(s)	83
BshFI	GGCC	5 cut(s)	73, 997, 1239, 1307, 1653
BshNI	GGYRCC	3 cut(s)	495, 913, 1013
BshTI	ACCGGT	1 cut(s)	78
BsiEI	CGRYCG	1 cut(s)	83
BsiSI	CCGG	3 cut(s)	79, 704, 1308
BslFI	GGGAC	1 cut(s)	245
BslI	CCNNNNNNNGG	4 cut(s)	269, 676, 703, 1093
BsmAI	GTCTC	3 cut(s)	119, 687, 1041
BsmFI	GGGAC	1 cut(s)	245
BsmI	GAATGC	1 cut(s)	1041
BsnI	GGCC	5 cut(s)	73, 997, 1239, 1307, 1653
Bso31I	GGTCTC	2 cut(s)	687, 1041
Bsp1286I	GDGCHC	1 cut(s)	498
Bsp143I	GATC	5 cut(s)	474, 888, 1354, 1450, 1582
Bsp1720I	GCTNAGC	1 cut(s)	630
BspACI	CCGC	3 cut(s)	225, 245, 1060
BspANI	GGCC	5 cut(s)	73, 997, 1239, 1307, 1653
BspCNI	CTCAG	5 cut(s)	107, 115, 139, 622, 1209
BspLI	GGNNCC	8 cut(s)	144, 260, 261, 497, 915, 1015, 1116, 1390
BspMAI	CTGCAG	1 cut(s)	1141
BspPI	GGATC	1 cut(s)	883
BspT107I	GGYRCC	3 cut(s)	495, 913, 1013
BspTI	CTTAAG	1 cut(s)	698
BspTNI	GGTCTC	2 cut(s)	687, 1041
BsrDI	GCAATG	2 cut(s)	1421, 1686
BsrFI	RCCGGY	1 cut(s)	78
BsrI	ACTGG	3 cut(s)	161, 307, 394
BssAI	RCCGGY	1 cut(s)	78
BssECI	CCNNGG	4 cut(s)	992, 1091, 1092, 1654
BssMI	GATC	5 cut(s)	474, 888, 1354, 1450, 1582
BssT1I	CCWWGG	1 cut(s)	992
Bst2UI	CCWGG	3 cut(s)	786, 1093, 1656
Bst4CI	ACNGT	4 cut(s)	326, 968, 1277, 1385
Bst6I	CTCTTC	2 cut(s)	248, 1557
BstAFI	CTTAAG	1 cut(s)	698
BstC8I	GCNNGC	5 cut(s)	406, 667, 848, 1237, 1365
BstDEI	CTNAG	8 cut(s)	94, 123, 138, 147, 630, 973, 1145, 1196
BstENI	CCTNNNNNAGG	1 cut(s)	267
BstF5I	GGATG	7 cut(s)	451, 460, 538, 736, 742, 1492, 1555
BstKTI	GATC	5 cut(s)	477, 891, 1357, 1453, 1585
BstMAI	GTCTC	3 cut(s)	119, 687, 1041
BstMBI	GATC	5 cut(s)	474, 888, 1354, 1450, 1582
BstMCI	CGRYCG	1 cut(s)	83
BstMWI	GCNNNNNNNGC	1 cut(s)	275
BstNI	CCWGG	3 cut(s)	786, 1093, 1656
BstSCI	CCNGG	3 cut(s)	784, 1091, 1654
BstSFI	CTRYAG	3 cut(s)	537, 645, 1137
BstSLI	GKGCMC	1 cut(s)	498
BstV1I	GCAGC	3 cut(s)	638, 1379, 1402
Bsu36I	CCTNAGG	1 cut(s)	138
BsuRI	GGCC	5 cut(s)	73, 997, 1239, 1307, 1653
BtsCI	GGATG	7 cut(s)	451, 460, 538, 736, 742, 1492, 1555
BtsIMutI	CAGTG	4 cut(s)	120, 1266, 1282, 1390
Cac8I	GCNNGC	5 cut(s)	406, 667, 848, 1237, 1365
CaiI	CAGNNNCTG	1 cut(s)	961
Cfr10I	RCCGGY	1 cut(s)	78
Cfr13I	GGNCC	5 cut(s)	20, 259, 857, 958, 1652
CseI	GACGC	2 cut(s)	247, 1608
Csp6I	GTAC	1 cut(s)	914
CspAI	ACCGGT	1 cut(s)	78
CviAII	CATG	4 cut(s)	478, 548, 1073, 1627
CviQI	GTAC	1 cut(s)	914
DdeI	CTNAG	8 cut(s)	94, 123, 138, 147, 630, 973, 1145, 1196
DpnI	GATC	5 cut(s)	476, 890, 1356, 1452, 1584
DpnII	GATC	5 cut(s)	474, 888, 1354, 1450, 1582
EaeI	YGGCCR	1 cut(s)	71
Eam1104I	CTCTTC	2 cut(s)	248, 1557
EarI	CTCTTC	2 cut(s)	248, 1557
Eco130I	CCWWGG	1 cut(s)	992
Eco31I	GGTCTC	2 cut(s)	687, 1041
Eco47I	GGWCC	4 cut(s)	20, 259, 857, 958
Eco57I	CTGAAG	4 cut(s)	855, 1242, 1491, 1666
Eco81I	CCTNAGG	1 cut(s)	138
EcoNI	CCTNNNNNAGG	1 cut(s)	267
EcoO109I	RGGNCCY	2 cut(s)	259, 958
EcoRII	CCWGG	3 cut(s)	784, 1091, 1654
EcoT14I	CCWWGG	1 cut(s)	992
EcoT22I	ATGCAT	1 cut(s)	690
ErhI	CCWWGG	1 cut(s)	992
FaeI	CATG	4 cut(s)	481, 551, 1076, 1630
FaqI	GGGAC	1 cut(s)	245
FatI	CATG	4 cut(s)	477, 547, 1072, 1626
FbaI	TGATCA	1 cut(s)	1582
Fnu4HI	GCNGC	4 cut(s)	627, 1061, 1368, 1416
FokI	GGATG	7 cut(s)	458, 467, 545, 743, 749, 1499, 1562
Fsp4HI	GCNGC	4 cut(s)	627, 1061, 1368, 1416
FspBI	CTAG	4 cut(s)	264, 341, 639, 999
GluI	GCNGC	4 cut(s)	627, 1061, 1368, 1416
GsuI	CTGGAG	2 cut(s)	895, 1355
HaeIII	GGCC	5 cut(s)	73, 997, 1239, 1307, 1653
HapII	CCGG	3 cut(s)	79, 704, 1308
HgaI	GACGC	2 cut(s)	247, 1608
Hin1II	CATG	4 cut(s)	481, 551, 1076, 1630
HindIII	AAGCTT	1 cut(s)	663
HinfI	GANTC	6 cut(s)	92, 149, 273, 983, 1133, 1340
HpaII	CCGG	3 cut(s)	79, 704, 1308
HphI	GGTGA	4 cut(s)	126, 144, 587, 980
Hpy166II	GTNNAC	1 cut(s)	100
Hpy188I	TCNGA	9 cut(s)	474, 988, 1009, 1046, 1199, 1222, 1345, 1431, 1510
Hpy8I	GTNNAC	1 cut(s)	100
HpyAV	CCTTC	2 cut(s)	376, 1657
HpyCH4III	ACNGT	4 cut(s)	326, 968, 1277, 1385
HpyCH4IV	ACGT	1 cut(s)	365
HpyF10VI	GCNNNNNNNGC	1 cut(s)	275
HpyF3I	CTNAG	8 cut(s)	94, 123, 138, 147, 630, 973, 1145, 1196
HpySE526I	ACGT	1 cut(s)	365
Hsp92II	CATG	4 cut(s)	481, 551, 1076, 1630
KflI	GGGWCCC	1 cut(s)	259
KpnI	GGTACC	1 cut(s)	917
Ksp22I	TGATCA	1 cut(s)	1582
Kzo9I	GATC	5 cut(s)	474, 888, 1354, 1450, 1582
LmnI	GCTCC	3 cut(s)	521, 1120, 1325
Lsp1109I	GCAGC	3 cut(s)	638, 1379, 1402
LweI	GCATC	5 cut(s)	523, 697, 727, 1435, 1620
MaeI	CTAG	4 cut(s)	264, 341, 639, 999
MaeII	ACGT	1 cut(s)	365
MaeIII	GTNAC	6 cut(s)	118, 150, 187, 288, 840, 968
MalI	GATC	5 cut(s)	476, 890, 1356, 1452, 1584
MboI	GATC	5 cut(s)	474, 888, 1354, 1450, 1582
MboII	GAAGA	6 cut(s)	37, 265, 1235, 1238, 1460, 1574
MfeI	CAATTG	1 cut(s)	414
MhlI	GDGCHC	1 cut(s)	498
MlsI	TGGCCA	1 cut(s)	73
MluNI	TGGCCA	1 cut(s)	73
MlyI	GAGTC	4 cut(s)	86, 158, 267, 1349
MmeI	TCCRAC	2 cut(s)	631, 790
Mox20I	TGGCCA	1 cut(s)	73
Mph1103I	ATGCAT	1 cut(s)	690
MroXI	GAANNNNTTC	1 cut(s)	41
MscI	TGGCCA	1 cut(s)	73
MseI	TTAA	6 cut(s)	311, 431, 516, 699, 905, 1154
MslI	CAYNNNNRTG	1 cut(s)	501
Msp20I	TGGCCA	1 cut(s)	73
MspA1I	CMGCKG	2 cut(s)	62, 629
MspCI	CTTAAG	1 cut(s)	698
MspI	CCGG	3 cut(s)	79, 704, 1308
MspR9I	CCNGG	3 cut(s)	786, 1093, 1656
MunI	CAATTG	1 cut(s)	414
Mva1269I	GAATGC	1 cut(s)	1041
MvaI	CCWGG	3 cut(s)	786, 1093, 1656
MwoI	GCNNNNNNNGC	1 cut(s)	275
NdeII	GATC	5 cut(s)	474, 888, 1354, 1450, 1582
NlaIII	CATG	4 cut(s)	481, 551, 1076, 1630
NlaIV	GGNNCC	8 cut(s)	144, 260, 261, 497, 915, 1015, 1116, 1390
NmuCI	GTSAC	4 cut(s)	118, 150, 187, 968
NsiI	ATGCAT	1 cut(s)	690
PasI	CCCWGGG	1 cut(s)	1092
PctI	GAATGC	1 cut(s)	1041
PdmI	GAANNNNTTC	1 cut(s)	41
PfeI	GAWTC	2 cut(s)	983, 1133
PflMI	CCANNNNNTGG	1 cut(s)	676
PinAI	ACCGGT	1 cut(s)	78
PkrI	GCNGC	4 cut(s)	628, 1062, 1369, 1417
PleI	GAGTC	4 cut(s)	86, 157, 267, 1348
PpsI	GAGTC	4 cut(s)	86, 157, 267, 1348
PpuMI	RGGWCCY	2 cut(s)	259, 958
Psp5II	RGGWCCY	2 cut(s)	259, 958
Psp6I	CCWGG	3 cut(s)	784, 1091, 1654
PspGI	CCWGG	3 cut(s)	784, 1091, 1654
PspN4I	GGNNCC	8 cut(s)	144, 260, 261, 497, 915, 1015, 1116, 1390
PspPI	GGNCC	5 cut(s)	20, 259, 857, 958, 1652
PspPPI	RGGWCCY	2 cut(s)	259, 958
PstI	CTGCAG	1 cut(s)	1141
PstNI	CAGNNNCTG	1 cut(s)	961
PvuII	CAGCTG	2 cut(s)	62, 629
RsaI	GTAC	1 cut(s)	915
RsaNI	GTAC	1 cut(s)	914
RseI	CAYNNNNRTG	1 cut(s)	501
SaqAI	TTAA	6 cut(s)	311, 431, 516, 699, 905, 1154
SatI	GCNGC	4 cut(s)	627, 1061, 1368, 1416
Sau3AI	GATC	5 cut(s)	474, 888, 1354, 1450, 1582
Sau96I	GGNCC	5 cut(s)	20, 259, 857, 958, 1652
SchI	GAGTC	4 cut(s)	86, 158, 267, 1349
ScrFI	CCNGG	3 cut(s)	786, 1093, 1656
SduI	GDGCHC	1 cut(s)	498
SfaNI	GCATC	5 cut(s)	523, 697, 727, 1435, 1620
SfcI	CTRYAG	3 cut(s)	537, 645, 1137
SinI	GGWCC	4 cut(s)	20, 259, 857, 958
SmiMI	CAYNNNNRTG	1 cut(s)	501
SmlI	CTYRAG	3 cut(s)	698, 1181, 1301
SmoI	CTYRAG	3 cut(s)	698, 1181, 1301
SsiI	CCGC	3 cut(s)	225, 245, 1060
SspI	AATATT	1 cut(s)	948
SspMI	CTAG	4 cut(s)	264, 341, 639, 999
StyD4I	CCNGG	3 cut(s)	784, 1091, 1654
StyI	CCWWGG	1 cut(s)	992
TaaI	ACNGT	4 cut(s)	326, 968, 1277, 1385
TaiI	ACGT	1 cut(s)	368
TaqI	TCGA	4 cut(s)	660, 691, 1377, 1560
TaqII	GACCGA	1 cut(s)	37
TauI	GCSGC	1 cut(s)	1063
TfiI	GAWTC	2 cut(s)	983, 1133
Tru1I	TTAA	6 cut(s)	311, 431, 516, 699, 905, 1154
Tru9I	TTAA	6 cut(s)	311, 431, 516, 699, 905, 1154
TscAI	CASTG	4 cut(s)	127, 1273, 1282, 1390
TseFI	GTSAC	4 cut(s)	118, 150, 187, 968
TseI	GCWGC	3 cut(s)	626, 1367, 1415
Tsp45I	GTSAC	4 cut(s)	118, 150, 187, 968
TspDTI	ATGAA	5 cut(s)	266, 536, 663, 1089, 1187
TspGWI	ACGGA	1 cut(s)	123
TspRI	CASTG	4 cut(s)	127, 1273, 1282, 1390
Van91I	CCANNNNNTGG	1 cut(s)	676
Vha464I	CTTAAG	1 cut(s)	698
VpaK11BI	GGWCC	4 cut(s)	20, 259, 857, 958
XagI	CCTNNNNNAGG	1 cut(s)	267
XapI	RAATTY	5 cut(s)	468, 709, 794, 1347, 1642
XmnI	GAANNNNTTC	1 cut(s)	41
XspI	CTAG	4 cut(s)	264, 341, 639, 999
Zsp2I	ATGCAT	1 cut(s)	690

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.