Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0002410

Physical Location & Seq

Genomic Coordinates Reverse (-)

27348819 .. 27352241

Gene Structure 3423 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00404850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGGGAAACAACTGTGTTGGAAATTCCAAGGACGGGCTTTTCCATTCGGTCTCAAATTCACTCTGGTGGACTCGATCAACCGGGGGTTTGGTTACTTCTACAACTAGAGAAATTAAAGCCCCTATTCAACCGACGAAGGTTCATCCTGAAAAGCAAAAGGAAGAGGAGACTAAACCAGCAGAAGAAGTAGCAATTCTGAAGGGAGAGACTAAACCTCAACAACCGGTAAATCATAGGGATCAAGAGACCAGACCAGCGCAACCCATAACACAAAATGGAGGGACTAATCAGCCAGCAGTACCTGTGAGACCAAAGAAGCCCCATAATGTCAAGAGGGTATCGAGTGCAGGGCTTCAGGCAGAGTCTGTCTTGCAAACCAAGACAGGTCATTTGAAGGAGTACTACAATTTGGGCCAGAAGCTTGGACATGGACAATTTGGGACAACTTTCCTTTGTGTGGAGAAAGCAACCGGAAAGGAATACGCATGCAAATCCATTGCCAAAAGGAAATTGTTGGCAACAGAAGATGTGGAGGATGTGAGGAGGGAGATTCAAATAATGCATCACTTGGCAGGGAATCCCAATGTCATATCGATTAAGGCGGCTTATGAGGATGCTGTTGCAGTTCATGTTGTTATGGAATTATGTGCAGGGGGTGAGCTCTTTGATAGGATTATCAAGAGGGGGCATTACTCCGAAAGAAAGGCAGCTCGGCTAGCAAGGACTATAGTTGGGGTTATAGAAGCTTGCCATTCTTTGGGTGTAATGCATCGCGATCTTAAACCCGAGAACTTTCTTTTTGTTGATGAGGAGGAGGATTCAGCACTCAAAGCTATAGATTTTGGGCTGTCAATATTTTTCAAGCCAGGTGAAACTTTCAATGATGTGGTTGGAAGCCCATATTATGTTGCACCTGAAGTTCTGCGCAAGCGGTATGGTCCAGAAGCAGATGTTTGGAGTGTTGGTGTTATCATTTACATTCTCTTGAGTGGGGTGCCTCCATTCTGGGGTGGTAAGAGATTTAAGATGTCTGAAACTGAGCAAGAGATCTTTGAAGAGGTTTTGCATGGTGATCTTGACTTCTCAACAGATCCCTGGCCTAATATTTCAGAAAGTGCGAAAGATTTAGTTAAGAAGATGCTTGTCAGAAACCCCAAAAAGCGGCTAACTGCTCATCAAGTTCTATGTCACCCATGGGTTCAGGTTGATGGAGTGGCTCCAGACAAGCCTCTTGATTCTGCAGTCTTAAGTCGCTTGAAGCAGTTCTCTGCAATGAACAAAATTAAGAAAATGGCTCTTAGAGTCATTGCTGAAAACCTCTCTGAAGAAGAAATTGCCGGTTTAAAAGAAATGTTCAAGATGATAGATACTGACAATAGTGGTCAAATTAGTTTCGAAGAACTGAAAGATGGACTGAAAAGATTTGGAGCTACTCTAAATGAGTCTGAAATATACGACCTGATGCAAGCTGCTGATGTTGACAACAGTGGCACAATTGATTATGGGGATGGCTATATAACTCAAGATGAACTTCAACAAGCGTGTGAGGAGTTTGGCATTCAGGATGTCCACTTGGAAGAGTTGATCCGAGAAGTCGATCAGGACAATGATGGTCGAATAGATTACAATGAGTTCGTTGCCATGATGCAGAAGGGCAATCCAGAGTTTGATAAGAAGGGTATTCAAACCAGTGCTCTTGGCATTGGATTTAGGGAGGCACTATCTGTTTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031984 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0098827 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

577

Amino Acids

64.41

Weight (kDa)

5.64

Isoelectric Point (pI)

44.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	135 - 400	8.7e-77	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	137 - 393	6e-22	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	448 - 474	9.5e-09	EF hand domain
EF-hand_7	PF13499	448 - 501	1.7e-10	EF-hand domain pair
EF-hand_6	PF13405	448 - 476	3.8e-07	EF-hand domain
EF-hand_5	PF13202	448 - 470	2.4e-06	EF hand
EF-hand_7	PF13499	502 - 550	3.2e-11	EF-hand domain pair
EF-hand_8	PF13833	503 - 551	5.8e-06	EF-hand domain pair
EF-hand_1	PF00036	525 - 551	4e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10660 AT3G10660 AT5G04870
fragaria_vesca	FvH4_6g20840 FvH4_6g20840 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490 FvH4_7g05490
malus_domestica	MD02G1257300.v1.1 MD07G1063300.v1.1 MD12G1044400.v1.1 MD14G1043300.v1.1
prunus_persica	Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.2G075100_v2.0.a1 Prupe.7G090800_v2.0.a1 Prupe.7G090800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0332341 RchiOBHm_Chr3g0464211 RchiOBHm_Chr3g0477181
rosa_laevigata	RLG00000023739 RLG00000029678
rosa_multiflora	Rmu_sc0000932.1_g000001 Rmu_sc0003422.1_g000006
rosa_roxburghii	Rroxscaffold_4G00318280 Rroxscaffold_6G00404350 Rroxscaffold_6G00404850
rosa_rugosa	Rorug01G0100000 Rorug03G0158600
rosa_samantha	Rh1CG118000 Rh1DG129100 Rh3AG208900 Rh3CG236600 Rh3DG235400
rosa_wichuraiana	Rw1G010080 Rw3G019050 Rw3G019440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	926
AccB1I	GGYRCC	1 cut(s)	994
AccB7I	CCANNNNNTGG	1 cut(s)	757
AccII	CGCG	1 cut(s)	774
AciI	CCGC	3 cut(s)	602, 931, 1162
AclWI	GGATC	3 cut(s)	246, 1085, 1579
AcsI	RAATTY	2 cut(s)	22, 55
AcuI	CTGAAG	4 cut(s)	218, 338, 936, 1344
AfaI	GTAC	2 cut(s)	300, 401
AfiI	CCNNNNNNNGG	5 cut(s)	33, 457, 757, 1007, 1161
AflII	CTTAAG	1 cut(s)	1246
AgeI	ACCGGT	1 cut(s)	223
AjnI	CCWGG	2 cut(s)	865, 1094
AleI	CACNNNNGTG	1 cut(s)	64
AluBI	AGCT	7 cut(s)	421, 661, 710, 746, 833, 1430, 1469
AluI	AGCT	7 cut(s)	421, 661, 710, 746, 833, 1430, 1469
Alw21I	GWGCWC	2 cut(s)	663, 1696
Alw26I	GTCTC	5 cut(s)	55, 161, 200, 239, 301
AlwI	GGATC	3 cut(s)	246, 1085, 1579
AlwNI	CAGNNNCTG	2 cut(s)	302, 365
Ama87I	CYCGRG	1 cut(s)	785
AoxI	GGCC	2 cut(s)	412, 1097
ApeKI	GCWGC	2 cut(s)	707, 1469
ApoI	RAATTY	2 cut(s)	22, 55
ArsI	GACNNNNNNTTYG	2 cut(s)	373, 405
AsiGI	ACCGGT	1 cut(s)	223
Asp700I	GAANNNNTTC	1 cut(s)	1262
AspLEI	GCGC	2 cut(s)	259, 927
AspS9I	GGNCC	2 cut(s)	412, 938
AsuC2I	CCSGG	1 cut(s)	82
AsuHPI	GGTGA	4 cut(s)	668, 881, 1082, 1181
AsuII	TTCGAA	1 cut(s)	1395
AsuNHI	GCTAGC	1 cut(s)	715
AvaI	CYCGRG	1 cut(s)	785
AvaII	GGWCC	1 cut(s)	938
BanI	GGYRCC	1 cut(s)	994
BanII	GRGCYC	1 cut(s)	663
BarI	GAAGNNNNNNTAC	2 cut(s)	386, 418
Bbv12I	GWGCWC	2 cut(s)	663, 1696
BbvI	GCAGC	2 cut(s)	719, 1456
BccI	CCATC	4 cut(s)	1202, 1403, 1502, 1604
BciT130I	CCWGG	2 cut(s)	867, 1096
BcnI	CCSGG	1 cut(s)	82
BcoDI	GTCTC	5 cut(s)	55, 161, 200, 239, 301
BfaI	CTAG	2 cut(s)	105, 716
BfmI	CTRYAG	3 cut(s)	726, 834, 1239
BfrI	CTTAAG	1 cut(s)	1246
BglII	AGATCT	1 cut(s)	1047
BisI	GCNGC	4 cut(s)	603, 708, 1163, 1470
BlsI	GCNGC	4 cut(s)	604, 709, 1164, 1471
BmcAI	AGTACT	1 cut(s)	401
Bme1390I	CCNGG	3 cut(s)	82, 867, 1096
Bme18I	GGWCC	1 cut(s)	938
BmeT110I	CYCGRG	1 cut(s)	785
BmgT120I	GGNCC	2 cut(s)	412, 938
BmiI	GGNNCC	2 cut(s)	996, 1218
BmrFI	CCNGG	3 cut(s)	82, 867, 1096
BmsI	GCATC	6 cut(s)	571, 604, 778, 1128, 1452, 1635
BmtI	GCTAGC	1 cut(s)	719
BpmI	CTGGAG	1 cut(s)	1203
Bpu14I	TTCGAA	1 cut(s)	1395
BpuEI	CTTGAG	2 cut(s)	1006, 1506
BpuMI	CCSGG	1 cut(s)	82
Bsa29I	ATCGAT	1 cut(s)	593
BsaI	GGTCTC	3 cut(s)	55, 239, 301
BsaJI	CCNNGG	4 cut(s)	27, 81, 1094, 1193
BsaWI	WCCGGW	2 cut(s)	223, 470
BsaXI	ACNNNNNCTCC	2 cut(s)	949, 979
Bsc4I	CCNNNNNNNGG	5 cut(s)	33, 457, 757, 1007, 1161
Bse118I	RCCGGY	2 cut(s)	223, 1337
Bse1I	ACTGG	1 cut(s)	1689
Bse3DI	GCAATG	3 cut(s)	495, 1278, 1305
BseBI	CCWGG	2 cut(s)	867, 1096
BseCI	ATCGAT	1 cut(s)	593
BseDI	CCNNGG	4 cut(s)	27, 81, 1094, 1193
BseGI	GGATG	5 cut(s)	142, 541, 619, 1513, 1570
BseLI	CCNNNNNNNGG	5 cut(s)	33, 457, 757, 1007, 1161
BseMI	GCAATG	3 cut(s)	495, 1278, 1305
BseMII	CTCAG	1 cut(s)	1029
BseNI	ACTGG	1 cut(s)	1689
BseRI	GAGGAG	5 cut(s)	179, 556, 824, 827, 1562
BseXI	GCAGC	2 cut(s)	719, 1456
BsgI	GTGCAG	2 cut(s)	366, 669
Bsh1236I	CGCG	1 cut(s)	774
BshFI	GGCC	2 cut(s)	414, 1099
BshNI	GGYRCC	1 cut(s)	994
BshTI	ACCGGT	1 cut(s)	223
BshVI	ATCGAT	1 cut(s)	593
BsiHKAI	GWGCWC	2 cut(s)	663, 1696
BsiHKCI	CYCGRG	1 cut(s)	785
BsiSI	CCGG	4 cut(s)	81, 224, 471, 1338
BslFI	GGGAC	2 cut(s)	295, 454
BslI	CCNNNNNNNGG	5 cut(s)	33, 457, 757, 1007, 1161
BsmAI	GTCTC	5 cut(s)	55, 161, 200, 239, 301
BsmFI	GGGAC	2 cut(s)	295, 454
BsmI	GAATGC	1 cut(s)	1557
BsnI	GGCC	2 cut(s)	414, 1099
Bso31I	GGTCTC	3 cut(s)	55, 239, 301
BsoBI	CYCGRG	1 cut(s)	785
Bsp119I	TTCGAA	1 cut(s)	1395
Bsp1286I	GDGCHC	2 cut(s)	663, 1696
Bsp143I	GATC	8 cut(s)	74, 238, 775, 1047, 1072, 1090, 1584, 1597
Bsp19I	CCATGG	1 cut(s)	1193
Bsp68I	TCGCGA	1 cut(s)	774
BspACI	CCGC	3 cut(s)	602, 931, 1162
BspANI	GGCC	2 cut(s)	414, 1099
BspCNI	CTCAG	1 cut(s)	1030
BspDI	ATCGAT	1 cut(s)	593
BspFNI	CGCG	1 cut(s)	774
BspLI	GGNNCC	2 cut(s)	996, 1218
BspMAI	CTGCAG	1 cut(s)	1243
BspOI	GCTAGC	1 cut(s)	719
BspPI	GGATC	3 cut(s)	246, 1085, 1579
BspT104I	TTCGAA	1 cut(s)	1395
BspT107I	GGYRCC	1 cut(s)	994
BspTI	CTTAAG	1 cut(s)	1246
BspTNI	GGTCTC	3 cut(s)	55, 239, 301
BsrDI	GCAATG	3 cut(s)	495, 1278, 1305
BsrFI	RCCGGY	2 cut(s)	223, 1337
BsrI	ACTGG	1 cut(s)	1689
BssAI	RCCGGY	2 cut(s)	223, 1337
BssECI	CCNNGG	4 cut(s)	27, 81, 1094, 1193
BssMI	GATC	8 cut(s)	74, 238, 775, 1047, 1072, 1090, 1584, 1597
BssT1I	CCWWGG	2 cut(s)	27, 1193
Bst2UI	CCWGG	2 cut(s)	867, 1096
Bst4CI	ACNGT	2 cut(s)	14, 1487
Bst6I	CTCTTC	3 cut(s)	156, 1050, 1572
BstAFI	CTTAAG	1 cut(s)	1246
BstBI	TTCGAA	1 cut(s)	1395
BstC8I	GCNNGC	6 cut(s)	294, 487, 717, 748, 929, 1467
BstDEI	CTNAG	2 cut(s)	1038, 1298
BstDSI	CCRYGG	1 cut(s)	1193
BstF5I	GGATG	5 cut(s)	142, 541, 619, 1513, 1570
BstFNI	CGCG	1 cut(s)	774
BstHHI	GCGC	2 cut(s)	259, 927
BstKTI	GATC	8 cut(s)	77, 241, 778, 1050, 1075, 1093, 1587, 1600
BstMAI	GTCTC	5 cut(s)	55, 161, 200, 239, 301
BstMBI	GATC	8 cut(s)	74, 238, 775, 1047, 1072, 1090, 1584, 1597
BstMWI	GCNNNNNNNGC	2 cut(s)	716, 830
BstNI	CCWGG	2 cut(s)	867, 1096
BstNSI	RCATGY	1 cut(s)	489
BstSCI	CCNGG	3 cut(s)	80, 865, 1094
BstSFI	CTRYAG	3 cut(s)	726, 834, 1239
BstUI	CGCG	1 cut(s)	774
BstV1I	GCAGC	2 cut(s)	719, 1456
BstX2I	RGATCY	2 cut(s)	1047, 1090
BstXI	CCANNNNNNTGG	1 cut(s)	422
BstYI	RGATCY	2 cut(s)	1047, 1090
Bsu15I	ATCGAT	1 cut(s)	593
BsuRI	GGCC	2 cut(s)	414, 1099
BsuTUI	ATCGAT	1 cut(s)	593
BtgI	CCRYGG	1 cut(s)	1193
BtgZI	GCGATG	1 cut(s)	755
BtsCI	GGATG	5 cut(s)	142, 541, 619, 1513, 1570
BtsIMutI	CAGTG	2 cut(s)	1492, 1696
BtuMI	TCGCGA	1 cut(s)	774
Cac8I	GCNNGC	6 cut(s)	294, 487, 717, 748, 929, 1467
CaiI	CAGNNNCTG	2 cut(s)	302, 365
CfoI	GCGC	2 cut(s)	259, 927
Cfr10I	RCCGGY	2 cut(s)	223, 1337
Cfr13I	GGNCC	2 cut(s)	412, 938
ClaI	ATCGAT	1 cut(s)	593
Csp6I	GTAC	2 cut(s)	299, 400
CspAI	ACCGGT	1 cut(s)	223
CviAII	CATG	6 cut(s)	428, 486, 629, 1067, 1194, 1642
CviQI	GTAC	2 cut(s)	299, 400
DdeI	CTNAG	2 cut(s)	1038, 1298
DpnI	GATC	8 cut(s)	76, 240, 777, 1049, 1074, 1092, 1586, 1599
DpnII	GATC	8 cut(s)	74, 238, 775, 1047, 1072, 1090, 1584, 1597
DraI	TTTAAA	1 cut(s)	1344
Eam1104I	CTCTTC	3 cut(s)	156, 1050, 1572
EarI	CTCTTC	3 cut(s)	156, 1050, 1572
Ecl136II	GAGCTC	1 cut(s)	661
Eco130I	CCWWGG	2 cut(s)	27, 1193
Eco24I	GRGCYC	1 cut(s)	663
Eco31I	GGTCTC	3 cut(s)	55, 239, 301
Eco47I	GGWCC	1 cut(s)	938
Eco53kI	GAGCTC	1 cut(s)	661
Eco57I	CTGAAG	4 cut(s)	218, 338, 936, 1344
Eco88I	CYCGRG	1 cut(s)	785
EcoICRI	GAGCTC	1 cut(s)	661
EcoRII	CCWGG	2 cut(s)	865, 1094
EcoT14I	CCWWGG	2 cut(s)	27, 1193
EcoT22I	ATGCAT	2 cut(s)	564, 771
EcoT38I	GRGCYC	1 cut(s)	663
ErhI	CCWWGG	2 cut(s)	27, 1193
FaeI	CATG	6 cut(s)	431, 489, 632, 1070, 1197, 1645
FalI	AAGNNNNNCTT	2 cut(s)	1515, 1547
FaqI	GGGAC	2 cut(s)	295, 454
FatI	CATG	6 cut(s)	427, 485, 628, 1066, 1193, 1641
Fnu4HI	GCNGC	4 cut(s)	603, 708, 1163, 1470
FokI	GGATG	5 cut(s)	129, 548, 626, 1520, 1577
FriOI	GRGCYC	1 cut(s)	663
Fsp4HI	GCNGC	4 cut(s)	603, 708, 1163, 1470
FspBI	CTAG	2 cut(s)	105, 716
FspI	TGCGCA	1 cut(s)	926
GlaI	GCGC	2 cut(s)	258, 926
GluI	GCNGC	4 cut(s)	603, 708, 1163, 1470
GsuI	CTGGAG	1 cut(s)	1203
HaeIII	GGCC	2 cut(s)	414, 1099
HapII	CCGG	4 cut(s)	81, 224, 471, 1338
HhaI	GCGC	2 cut(s)	259, 927
Hin1II	CATG	6 cut(s)	431, 489, 632, 1070, 1197, 1645
Hin6I	GCGC	2 cut(s)	257, 925
HinP1I	GCGC	2 cut(s)	257, 925
HincII	GTYRAC	1 cut(s)	1480
HindII	GTYRAC	1 cut(s)	1480
HindIII	AAGCTT	2 cut(s)	419, 744
HinfI	GANTC	8 cut(s)	70, 362, 550, 577, 818, 1235, 1302, 1442
HpaII	CCGG	4 cut(s)	81, 224, 471, 1338
HphI	GGTGA	4 cut(s)	668, 881, 1082, 1181
Hpy166II	GTNNAC	3 cut(s)	69, 1480, 1570
Hpy188I	TCNGA	8 cut(s)	198, 697, 1033, 1111, 1148, 1324, 1447, 1589
Hpy8I	GTNNAC	3 cut(s)	69, 1480, 1570
Hpy99I	CGWCG	1 cut(s)	136
HpyAV	CCTTC	5 cut(s)	130, 193, 388, 1645, 1669
HpyCH4III	ACNGT	2 cut(s)	14, 1487
HpyF10VI	GCNNNNNNNGC	2 cut(s)	716, 830
HpyF3I	CTNAG	2 cut(s)	1038, 1298
Hsp92II	CATG	6 cut(s)	431, 489, 632, 1070, 1197, 1645
HspAI	GCGC	2 cut(s)	257, 925
Kzo9I	GATC	8 cut(s)	74, 238, 775, 1047, 1072, 1090, 1584, 1597
LmnI	GCTCC	2 cut(s)	1222, 1427
Lsp1109I	GCAGC	2 cut(s)	719, 1456
LweI	GCATC	6 cut(s)	571, 604, 778, 1128, 1452, 1635
MaeI	CTAG	2 cut(s)	105, 716
MaeIII	GTNAC	2 cut(s)	91, 1187
MalI	GATC	8 cut(s)	76, 240, 777, 1049, 1074, 1092, 1586, 1599
MboI	GATC	8 cut(s)	74, 238, 775, 1047, 1072, 1090, 1584, 1597
MboII	GAAGA	9 cut(s)	173, 194, 536, 1067, 1147, 1337, 1340, 1409, 1589
MfeI	CAATTG	1 cut(s)	1494
MflI	RGATCY	2 cut(s)	1047, 1090
MhlI	GDGCHC	2 cut(s)	663, 1696
MlyI	GAGTC	4 cut(s)	64, 371, 1311, 1451
MmeI	TCCRAC	1 cut(s)	871
Mph1103I	ATGCAT	2 cut(s)	564, 771
MroXI	GAANNNNTTC	1 cut(s)	1262
MseI	TTAA	9 cut(s)	114, 597, 780, 1023, 1131, 1247, 1284, 1343, 1732
MslI	CAYNNNNRTG	1 cut(s)	64
MspCI	CTTAAG	1 cut(s)	1246
MspI	CCGG	4 cut(s)	81, 224, 471, 1338
MspR9I	CCNGG	3 cut(s)	82, 867, 1096
MunI	CAATTG	1 cut(s)	1494
Mva1269I	GAATGC	1 cut(s)	1557
MvaI	CCWGG	2 cut(s)	867, 1096
MvnI	CGCG	1 cut(s)	774
MwoI	GCNNNNNNNGC	2 cut(s)	716, 830
NciI	CCSGG	1 cut(s)	82
NcoI	CCATGG	1 cut(s)	1193
NdeII	GATC	8 cut(s)	74, 238, 775, 1047, 1072, 1090, 1584, 1597
NheI	GCTAGC	1 cut(s)	715
NlaIII	CATG	6 cut(s)	431, 489, 632, 1070, 1197, 1645
NlaIV	GGNNCC	2 cut(s)	996, 1218
NmeAIII	GCCGAG	1 cut(s)	691
NmuCI	GTSAC	1 cut(s)	1187
NruI	TCGCGA	1 cut(s)	774
NsbI	TGCGCA	1 cut(s)	926
NsiI	ATGCAT	2 cut(s)	564, 771
NspI	RCATGY	1 cut(s)	489
NspV	TTCGAA	1 cut(s)	1395
OliI	CACNNNNGTG	1 cut(s)	64
PaeI	GCATGC	1 cut(s)	489
PctI	GAATGC	1 cut(s)	1557
PdmI	GAANNNNTTC	1 cut(s)	1262
PfeI	GAWTC	4 cut(s)	550, 577, 818, 1235
PflMI	CCANNNNNTGG	1 cut(s)	757
PinAI	ACCGGT	1 cut(s)	223
PkrI	GCNGC	4 cut(s)	604, 709, 1164, 1471
PleI	GAGTC	4 cut(s)	64, 370, 1310, 1450
PpsI	GAGTC	4 cut(s)	64, 370, 1310, 1450
Psp124BI	GAGCTC	1 cut(s)	663
Psp6I	CCWGG	2 cut(s)	865, 1094
PspGI	CCWGG	2 cut(s)	865, 1094
PspN4I	GGNNCC	2 cut(s)	996, 1218
PspPI	GGNCC	2 cut(s)	412, 938
PstI	CTGCAG	1 cut(s)	1243
PstNI	CAGNNNCTG	2 cut(s)	302, 365
PsuI	RGATCY	2 cut(s)	1047, 1090
RruI	TCGCGA	1 cut(s)	774
RsaI	GTAC	2 cut(s)	300, 401
RsaNI	GTAC	2 cut(s)	299, 400
RseI	CAYNNNNRTG	1 cut(s)	64
SacI	GAGCTC	1 cut(s)	663
SaqAI	TTAA	9 cut(s)	114, 597, 780, 1023, 1131, 1247, 1284, 1343, 1732
SatI	GCNGC	4 cut(s)	603, 708, 1163, 1470
Sau3AI	GATC	8 cut(s)	74, 238, 775, 1047, 1072, 1090, 1584, 1597
Sau96I	GGNCC	2 cut(s)	412, 938
ScaI	AGTACT	1 cut(s)	401
SchI	GAGTC	4 cut(s)	64, 371, 1311, 1451
ScrFI	CCNGG	3 cut(s)	82, 867, 1096
SduI	GDGCHC	2 cut(s)	663, 1696
SfaNI	GCATC	6 cut(s)	571, 604, 778, 1128, 1452, 1635
SfcI	CTRYAG	3 cut(s)	726, 834, 1239
SfuI	TTCGAA	1 cut(s)	1395
SinI	GGWCC	1 cut(s)	938
SmiMI	CAYNNNNRTG	1 cut(s)	64
SmlI	CTYRAG	3 cut(s)	985, 1246, 1521
SmoI	CTYRAG	3 cut(s)	985, 1246, 1521
SphI	GCATGC	1 cut(s)	489
SsiI	CCGC	3 cut(s)	602, 931, 1162
SspI	AATATT	2 cut(s)	855, 1105
SspMI	CTAG	2 cut(s)	105, 716
SstI	GAGCTC	1 cut(s)	663
StyD4I	CCNGG	3 cut(s)	80, 865, 1094
StyI	CCWWGG	2 cut(s)	27, 1193
TaaI	ACNGT	2 cut(s)	14, 1487
TaqI	TCGA	6 cut(s)	73, 341, 593, 1395, 1596, 1615
TaqII	GACCGA	1 cut(s)	37
TatI	WGTACW	1 cut(s)	399
TauI	GCSGC	2 cut(s)	605, 1165
TfiI	GAWTC	4 cut(s)	550, 577, 818, 1235
Tru1I	TTAA	9 cut(s)	114, 597, 780, 1023, 1131, 1247, 1284, 1343, 1732
Tru9I	TTAA	9 cut(s)	114, 597, 780, 1023, 1131, 1247, 1284, 1343, 1732
TscAI	CASTG	2 cut(s)	1492, 1696
TseFI	GTSAC	1 cut(s)	1187
TseI	GCWGC	2 cut(s)	707, 1469
Tsp45I	GTSAC	1 cut(s)	1187
TspDTI	ATGAA	4 cut(s)	131, 617, 1289, 1542
TspRI	CASTG	2 cut(s)	1492, 1696
Van91I	CCANNNNNTGG	1 cut(s)	757
Vha464I	CTTAAG	1 cut(s)	1246
VpaK11BI	GGWCC	1 cut(s)	938
XapI	RAATTY	2 cut(s)	22, 55
XceI	RCATGY	1 cut(s)	489
XmnI	GAANNNNTTC	1 cut(s)	1262
XspI	CTAG	2 cut(s)	105, 716
ZrmI	AGTACT	1 cut(s)	401
Zsp2I	ATGCAT	2 cut(s)	564, 771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.