Rw3G019450

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0006819
Physical Location & Seq
Forward (+)
22090655 .. 22092767
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G019450.1

Sequence Viewer

Length: 1281 bp
ATGGAGTCTGAAAATGGGATTGCTTTAGGGGATGAGAATTGTGCTGTTGTGGAAAAACATGTAGAAGAATCAGTACCGGATTTGAACATTGAAGGAAAGAATGTTGATGCTAATATAGAAGTTCCTACCACGAATGGAGTGTCTGAACCTGTAAGCAAAGGTGAAGGCATTAACCCTTCAGGAGGTGCAGTTGAAGTTGCCGCAAGTGTTCCGCCTGGTAAAAATTCAAAAACAAAAAAGGATCCTCATGCTCCAAACAATGGTGTTTCAAAGGGAAGGTTGGCCAAGGATAAACCCAGTGAGAAAGGTGCAGCTCCAGTTTCTCGTACACAAAGGGCAATACTTTCTCAGAGTCTTTCGTTCCCAGCCAGGGGAGCTCGTGTTGATGCTATGAAGAAGAGCATTGATGTTTATCCAGTGAAAACAGAAGCCAAACGCACTCGAGGGAATGCAACCAAAGCTGAGGCCCCTTTTCCATCTTCTCGTTTGAATCATCAAAATAGGCGTGCATCCACAGGAATACACTCAAATGATGAGAACAAAGCTGCTGGAGCTTCAGTTAGGAGGACTACTTTAGCATCAATAACTAGTATTAGATCCTCTGCATCAATGAAGTCTGGCACTGTGAGTAAACCTGCTAATAGTTCATATGATGTGACCCGATCAGTTGGGCAGATCTTAAGTGCTGTAAAAACAACCCTGCCAATCAAAGAGGATGATGATGCTCATTCCATTGCTTCAAGCACTACACCTGGTGGGCGGAGGAGCAGTGGTTCTGCATTTTCCTTCAGGTTGGATGAACGTGCTGAAAAAAGGAAGGAGTTCTTTACAAAGCTAGAAGAGAAGATACAGGCTAAGGAAGCAGAAAAAAATAATTCGCAAGCAAAATCAAAGGAAAACCAGGAAGCAGAGATCAAGCAACTGAGGAAGAGCCTGACATTTAGGGCAGCTCCCATGCCAACTTTCTATAAAGAGCCTCCTCCAAAAATTGAAATAAAGAAGATACCGACCACACGTCCCATATCTCCAAAGCTTGGAAGGCACAAGAACTCCATTTCTTCATTGAACAACCCCTCAGAAGGTGGTGGGACGTGTCTTAGCCCGCGTCTGAATCGAGAACAGAACAATTCAAACAAAGGGTTAAAGGCAACAGGTGAAAAAGATGTCACAGAATCAAAGAAGCCTATCAGGAAGNATACCTGCTGCAAATGTGCCTGCAAGGATAGATGTCCCTGTAGGATGTGTCGTCCCAGATGGACGAGGTACGACCATGGCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

426

Amino Acids

45.94

Weight (kDa)

9.8

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 261 - 330 3.5e-25 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 643, 1208
AccB7I CCANNNNNTGG 2 cut(s) 260, 1034
AccII CGCG 1 cut(s) 1105
AciI CCGC 5 cut(s) 201, 212, 760, 1103, 1275
AclWI GGATC 3 cut(s) 236, 249, 591
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 3 cut(s) 162, 540, 772
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 3 cut(s) 75, 328, 1265
AfiI CCNNNNNNNGG 6 cut(s) 182, 260, 370, 371, 1034, 1079
AflII CTTAAG 1 cut(s) 679
AflIII ACRYGT 3 cut(s) 58, 1013, 1091
AhdI GACNNNNNGTC 1 cut(s) 1014
AhlI ACTAGT 1 cut(s) 587
AjiI CACGTC 2 cut(s) 1016, 1092
AjnI CCWGG 4 cut(s) 214, 368, 751, 900
AloI GAACNNNNNNTCC 2 cut(s) 757, 789
AluBI AGCT 8 cut(s) 314, 377, 461, 545, 554, 835, 950, 1033
AluI AGCT 8 cut(s) 314, 377, 461, 545, 554, 835, 950, 1033
Alw21I GWGCWC 1 cut(s) 379
AlwI GGATC 3 cut(s) 236, 249, 591
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 2 cut(s) 282, 465
ApeKI GCWGC 4 cut(s) 311, 545, 947, 1203
ApoI RAATTY 1 cut(s) 223
ArsI GACNNNNNNTTYG 2 cut(s) 125, 157
Asp700I GAANNNNTTC 1 cut(s) 821
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 2 cut(s) 173, 1166
AvaI CYCGRG 1 cut(s) 441
BalI TGGCCA 1 cut(s) 284
BamHI GGATCC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 379
BarI GAAGNNNNNNTAC 4 cut(s) 57, 89, 831, 863
BauI CACGAG 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 379
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 4 cut(s) 323, 532, 959, 1190
BccI CCATC 2 cut(s) 484, 1248
BciT130I CCWGG 4 cut(s) 216, 370, 753, 902
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 2 cut(s) 588, 836
BfmI CTRYAG 1 cut(s) 1234
BfrI CTTAAG 1 cut(s) 679
BfuAI ACCTGC 2 cut(s) 643, 1208
BglII AGATCT 1 cut(s) 675
BisI GCNGC 6 cut(s) 201, 312, 546, 948, 1204, 1276
BlsI GCNGC 6 cut(s) 202, 313, 547, 949, 1205, 1277
Bme1390I CCNGG 4 cut(s) 216, 370, 753, 902
BmeRI GACNNNNNGTC 1 cut(s) 1014
BmeT110I CYCGRG 1 cut(s) 441
BmgBI CACGTC 2 cut(s) 1016, 1092
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 2 cut(s) 243, 468
BmrFI CCNGG 4 cut(s) 216, 370, 753, 902
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
BmuI ACTGGG 1 cut(s) 291
BpmI CTGGAG 2 cut(s) 300, 570
Bpu10I CCTNAGC 2 cut(s) 462, 855
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 285, 369, 1270
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 6 cut(s) 182, 260, 370, 371, 1034, 1079
Bse1I ACTGG 3 cut(s) 297, 317, 416
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 4 cut(s) 216, 370, 753, 902
BseDI CCNNGG 3 cut(s) 285, 369, 1270
BseGI GGATG 5 cut(s) 37, 509, 721, 802, 1245
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 6 cut(s) 182, 260, 370, 371, 1034, 1079
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 4 cut(s) 362, 453, 914, 1089
BseNI ACTGG 3 cut(s) 297, 317, 416
BseRI GAGGAG 2 cut(s) 778, 969
BseXI GCAGC 4 cut(s) 323, 532, 959, 1190
BseYI CCCAGC 1 cut(s) 364
BsgI GTGCAG 2 cut(s) 207, 330
Bsh1236I CGCG 1 cut(s) 1105
BshFI GGCC 2 cut(s) 284, 467
BsiHKAI GWGCWC 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 4 cut(s) 1002, 1102, 1215, 1233
BslI CCNNNNNNNGG 6 cut(s) 182, 260, 370, 371, 1034, 1079
BsmFI GGGAC 4 cut(s) 1002, 1102, 1215, 1233
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 2 cut(s) 284, 467
BsoBI CYCGRG 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 379
Bsp143I GATC 5 cut(s) 241, 596, 662, 675, 912
Bsp19I CCATGG 1 cut(s) 1270
BspACI CCGC 5 cut(s) 201, 212, 760, 1103, 1275
BspANI GGCC 2 cut(s) 284, 467
BspCNI CTCAG 4 cut(s) 361, 454, 915, 1088
BspFNI CGCG 1 cut(s) 1105
BspLI GGNNCC 2 cut(s) 243, 468
BspMI ACCTGC 2 cut(s) 643, 1208
BspPI GGATC 3 cut(s) 236, 249, 591
BspQI GCTCTTC 2 cut(s) 392, 923
BspTI CTTAAG 1 cut(s) 679
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 3 cut(s) 297, 317, 416
BssECI CCNNGG 3 cut(s) 285, 369, 1270
BssMI GATC 5 cut(s) 241, 596, 662, 675, 912
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 2 cut(s) 285, 1270
Bst2BI CACGAG 1 cut(s) 378
Bst2UI CCWGG 4 cut(s) 216, 370, 753, 902
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 3 cut(s) 392, 834, 923
BstAFI CTTAAG 1 cut(s) 679
BstC8I GCNNGC 4 cut(s) 507, 882, 1103, 1216
BstDEI CTNAG 6 cut(s) 348, 462, 855, 923, 1075, 1097
BstDSI CCRYGG 1 cut(s) 1270
BstENI CCTNNNNNAGG 1 cut(s) 180
BstF5I GGATG 5 cut(s) 37, 509, 721, 802, 1245
BstFNI CGCG 1 cut(s) 1105
BstKTI GATC 5 cut(s) 244, 599, 665, 678, 915
BstMBI GATC 5 cut(s) 241, 596, 662, 675, 912
BstMWI GCNNNNNNNGC 5 cut(s) 374, 458, 551, 860, 1039
BstNI CCWGG 4 cut(s) 216, 370, 753, 902
BstNSI RCATGY 1 cut(s) 62
BstSCI CCNGG 4 cut(s) 214, 368, 751, 900
BstSFI CTRYAG 1 cut(s) 1234
BstUI CGCG 1 cut(s) 1105
BstV1I GCAGC 4 cut(s) 323, 532, 959, 1190
BstX2I RGATCY 3 cut(s) 241, 596, 675
BstYI RGATCY 3 cut(s) 241, 596, 675
BsuRI GGCC 2 cut(s) 284, 467
BtgI CCRYGG 1 cut(s) 1270
BtrI CACGTC 2 cut(s) 1016, 1092
BtsCI GGATG 5 cut(s) 37, 509, 721, 802, 1245
BtsI GCAGTG 1 cut(s) 775
BtsIMutI CAGTG 4 cut(s) 304, 423, 621, 775
BveI ACCTGC 2 cut(s) 643, 1208
Cac8I GCNNGC 4 cut(s) 507, 882, 1103, 1216
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 1 cut(s) 1094
CsiI ACCWGGT 1 cut(s) 751
Csp6I GTAC 3 cut(s) 74, 327, 1264
CviAII CATG 4 cut(s) 59, 248, 955, 1271
CviQI GTAC 3 cut(s) 74, 327, 1264
DdeI CTNAG 6 cut(s) 348, 462, 855, 923, 1075, 1097
DpnI GATC 5 cut(s) 243, 598, 664, 677, 914
DpnII GATC 5 cut(s) 241, 596, 662, 675, 912
DraIII CACNNNGTG 1 cut(s) 755
DriI GACNNNNNGTC 1 cut(s) 1014
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 3 cut(s) 392, 834, 923
Eam1105I GACNNNNNGTC 1 cut(s) 1014
EarI CTCTTC 3 cut(s) 392, 834, 923
EciI GGCGGA 2 cut(s) 201, 775
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 2 cut(s) 285, 1270
Eco24I GRGCYC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 377
Eco57I CTGAAG 3 cut(s) 162, 540, 772
Eco88I CYCGRG 1 cut(s) 441
EcoICRI GAGCTC 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 4 cut(s) 214, 368, 751, 900
EcoT14I CCWWGG 2 cut(s) 285, 1270
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 2 cut(s) 285, 1270
FaeI CATG 4 cut(s) 62, 251, 958, 1274
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 4 cut(s) 1002, 1102, 1215, 1233
FatI CATG 4 cut(s) 58, 247, 954, 1270
FauI CCCGC 1 cut(s) 1110
FauNDI CATATG 1 cut(s) 649
Fnu4HI GCNGC 6 cut(s) 201, 312, 546, 948, 1204, 1276
FokI GGATG 5 cut(s) 44, 496, 728, 809, 1252
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 6 cut(s) 201, 312, 546, 948, 1204, 1276
FspBI CTAG 2 cut(s) 588, 836
GluI GCNGC 6 cut(s) 201, 312, 546, 948, 1204, 1276
GsaI CCCAGC 1 cut(s) 368
GsuI CTGGAG 2 cut(s) 300, 570
HaeIII GGCC 2 cut(s) 284, 467
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 1094
Hin1II CATG 4 cut(s) 62, 251, 958, 1274
HindIII AAGCTT 1 cut(s) 1031
HinfI GANTC 6 cut(s) 5, 68, 352, 490, 1111, 1172
HpaII CCGG 1 cut(s) 77
HphI GGTGA 2 cut(s) 173, 1166
Hpy166II GTNNAC 2 cut(s) 329, 632
Hpy188I TCNGA 5 cut(s) 10, 145, 351, 1078, 1110
Hpy188III TCNNGA 3 cut(s) 180, 1115, 1189
Hpy8I GTNNAC 2 cut(s) 329, 632
HpyAV CCTTC 8 cut(s) 86, 158, 186, 270, 796, 811, 1032, 1073
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 3 cut(s) 802, 1015, 1091
HpyCH4V TGCA 8 cut(s) 188, 311, 452, 509, 605, 779, 1206, 1218
HpyF10VI GCNNNNNNNGC 5 cut(s) 374, 458, 551, 860, 1039
HpyF3I CTNAG 6 cut(s) 348, 462, 855, 923, 1075, 1097
HpySE526I ACGT 3 cut(s) 802, 1015, 1091
Hsp92II CATG 4 cut(s) 62, 251, 958, 1274
Kzo9I GATC 5 cut(s) 241, 596, 662, 675, 912
LguI GCTCTTC 2 cut(s) 392, 923
LmnI GCTCC 6 cut(s) 256, 319, 374, 551, 765, 955
Lsp1109I GCAGC 4 cut(s) 323, 532, 959, 1190
LweI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
MabI ACCWGGT 1 cut(s) 751
MaeI CTAG 2 cut(s) 588, 836
MaeII ACGT 3 cut(s) 802, 1015, 1091
MaeIII GTNAC 2 cut(s) 655, 1165
MalI GATC 5 cut(s) 243, 598, 664, 677, 914
MboI GATC 5 cut(s) 241, 596, 662, 675, 912
MboII GAAGA 9 cut(s) 77, 406, 409, 471, 851, 856, 940, 1012, 1050
MflI RGATCY 3 cut(s) 241, 596, 675
MhlI GDGCHC 1 cut(s) 379
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 5 cut(s) 37, 223, 874, 987, 1126
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 2 cut(s) 14, 361
MmeI TCCRAC 1 cut(s) 774
Mox20I TGGCCA 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 821
MscI TGGCCA 1 cut(s) 284
MseI TTAA 3 cut(s) 171, 680, 1142
MslI CAYNNNNRTG 1 cut(s) 528
Msp20I TGGCCA 1 cut(s) 284
MspCI CTTAAG 1 cut(s) 679
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 4 cut(s) 216, 370, 753, 902
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 4 cut(s) 216, 370, 753, 902
MvnI CGCG 1 cut(s) 1105
MwoI GCNNNNNNNGC 5 cut(s) 374, 458, 551, 860, 1039
NcoI CCATGG 1 cut(s) 1270
NdeI CATATG 1 cut(s) 649
NdeII GATC 5 cut(s) 241, 596, 662, 675, 912
NlaIII CATG 4 cut(s) 62, 251, 958, 1274
NlaIV GGNNCC 2 cut(s) 243, 468
NmuCI GTSAC 2 cut(s) 655, 1165
NspI RCATGY 1 cut(s) 62
PaeR7I CTCGAG 1 cut(s) 441
PciI ACATGT 1 cut(s) 58
PciSI GCTCTTC 2 cut(s) 392, 923
PctI GAATGC 1 cut(s) 454
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 4 cut(s) 68, 490, 1111, 1172
PflMI CCANNNNNTGG 2 cut(s) 260, 1034
PkrI GCNGC 6 cut(s) 202, 313, 547, 949, 1205, 1277
PleI GAGTC 2 cut(s) 13, 360
PpsI GAGTC 2 cut(s) 13, 360
PscI ACATGT 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 379
Psp6I CCWGG 4 cut(s) 214, 368, 751, 900
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 4 cut(s) 214, 368, 751, 900
PspN4I GGNNCC 2 cut(s) 243, 468
PspPI GGNCC 1 cut(s) 466
PspXI VCTCGAGB 1 cut(s) 441
PsuI RGATCY 3 cut(s) 241, 596, 675
RsaI GTAC 3 cut(s) 75, 328, 1265
RsaNI GTAC 3 cut(s) 74, 327, 1264
RseI CAYNNNNRTG 1 cut(s) 528
SacI GAGCTC 1 cut(s) 379
SapI GCTCTTC 2 cut(s) 392, 923
SaqAI TTAA 3 cut(s) 171, 680, 1142
SatI GCNGC 6 cut(s) 201, 312, 546, 948, 1204, 1276
Sau3AI GATC 5 cut(s) 241, 596, 662, 675, 912
Sau96I GGNCC 1 cut(s) 466
SchI GAGTC 2 cut(s) 14, 361
ScrFI CCNGG 4 cut(s) 216, 370, 753, 902
SduI GDGCHC 1 cut(s) 379
SexAI ACCWGGT 1 cut(s) 751
SfaNI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
SfcI CTRYAG 1 cut(s) 1234
Sfr274I CTCGAG 1 cut(s) 441
SlaI CTCGAG 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 2 cut(s) 441, 679
SmoI CTYRAG 2 cut(s) 441, 679
SpeI ACTAGT 1 cut(s) 587
Sse9I AATT 5 cut(s) 37, 223, 874, 987, 1126
SsiI CCGC 5 cut(s) 201, 212, 760, 1103, 1275
SspMI CTAG 2 cut(s) 588, 836
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 4 cut(s) 214, 368, 751, 900
StyI CCWWGG 2 cut(s) 285, 1270
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 3 cut(s) 805, 1018, 1094
TaqI TCGA 2 cut(s) 442, 1114
TasI AATT 5 cut(s) 37, 223, 874, 987, 1126
TauI GCSGC 2 cut(s) 203, 1278
TfiI GAWTC 4 cut(s) 68, 490, 1111, 1172
Tru1I TTAA 3 cut(s) 171, 680, 1142
Tru9I TTAA 3 cut(s) 171, 680, 1142
TscAI CASTG 4 cut(s) 304, 423, 628, 775
TseFI GTSAC 2 cut(s) 655, 1165
TseI GCWGC 4 cut(s) 311, 545, 947, 1203
Tsp45I GTSAC 2 cut(s) 655, 1165
TspDTI ATGAA 5 cut(s) 407, 626, 636, 813, 1050
TspRI CASTG 4 cut(s) 304, 423, 628, 775
Van91I CCANNNNNTGG 2 cut(s) 260, 1034
Vha464I CTTAAG 1 cut(s) 679
XagI CCTNNNNNAGG 1 cut(s) 180
XapI RAATTY 1 cut(s) 223
XceI RCATGY 1 cut(s) 62
XhoI CTCGAG 1 cut(s) 441
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 2 cut(s) 588, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.