MD14G1043400.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006819
Physical Location & Seq
Forward (+)
4092113 .. 4096629
4517 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1043400.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGGATATTTCACAAATGGAGTCTGAAAATGGGCTTACATTTGAGGATGAGAGTTGTGTTATTGAGGAAAAACATGTAGATGTATCAGTACCGGATTTGAGCAAACAGGGCAAGAATGCAGATGGTAATATAGAAGTTCCTGCGGTGAACGGAATATCTGAACCTGTGACAAAAGATGAAGGCATTAACTCTTCTGAAGTTGTAGTCACAGCCACTGCGACTGTTCAGCCTGGTAAAAATTCAAAAACTAGAAAGAATCTTCATGCTCCGAACAATGGTTTTTCAAAGAGCAAACTGGCCAAGGATAAACCTATTGTGAAAGACGCAACTCCATTCCCTCGTAAGGAGAGGGCAACACTTTCTCAGAGTCAGTCTTTCCCAGCAAAAGGATCTCGTACAGATCCTATGAAAAACAGCATTGATGTGTACCCAGTGAAAACAGAAGTTAAACGCGCTCGAGGAAAAGCAACTAAAGTTGAGGCTCCCATTTCAGTTTCTCGTCTGAATAATCCAACTAGGCGTGCCTCCACAGGAGTTCATTCAAAGGATGGGAACAAAACTGGTGGAGCTGCCATTAAGAGGACTTCTTTAGCATCAATACCCAGCATTAGATCCTCTCCATCTGGCAAGTCTGGTTCTGTGGATACATCCGCCAATAGCCCATCTAATGTGATCCAATCAGTTGATCAAAGTTTAAATGCTGTAAAAACAACTCTGCCAATCAAAGAGGACGATGATGCTCACTCCGTTGCTTCTACTACACCTAATGGGCGGAGGATCAGCGCTTCTGGATTTTCCTTCAGGTTGGAAGAACGTGCTGAAAAACGGAAGGAGTTTTTTACAAAGCTAGAAGAGAAGATACAGGCTAAGGAAGAAGAAAAAACTAACTCGCAAGCAAAATCAAAGGAGAGTCAGGAAGCAGAGATCAAGCGACTAAGGAAGAGCCTGATGTTTAAGGCGGCGCCCATGCCAAGTTTCTATAAAGAGCCTCCTCCGAAAGTTGAACTCAAGAAGATTCCTACCACACGTCCAAAATCTCCGAAGCTTGGAAGGAACAAGAGCTCCATTTCTTCACTAAACAATTCTTCAGAAGTTGCTGGGGTGTCTCTTAGCCCACGTCTGAATCGAGAACTGAATAATTCAAAGAAAGGATTAAAGACTAAAAGCGAAAAGGACGTCATAGATCCAAAGAAGCCTATTAAAAAGTCTCAAACTAGGCTTCATTCTCAGGAAACTGCTGCTACCAAAACCGAAGCAACGGGTACCGAAGAAGAAACACACGACCAGAAAGCGCGCACTGGAAAAACTGCAGAAGCTCAGGGCCAATCAGGGCATCTTCCCGAATTCAAAGATGAGATAGAGCCTGCAGTGAGTGTTGGCCAGATCGAAGAACCAGTCCTCGGTGCACCTATCCCCGAGATCATGCCCCATGAAGTTTCAGTTGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

484

Amino Acids

52.31

Weight (kDa)

9.51

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 265 - 339 1.9e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1179
Acc65I GGTACC 1 cut(s) 1262
AccB1I GGYRCC 2 cut(s) 961, 1262
AccII CGCG 2 cut(s) 453, 1294
AciI CCGC 4 cut(s) 143, 651, 772, 959
AclWI GGATC 6 cut(s) 395, 397, 606, 667, 785, 1178
AcoI YGGCCR 2 cut(s) 297, 1378
AcsI RAATTY 2 cut(s) 238, 1343
AcuI CTGAAG 3 cut(s) 216, 784, 1071
AcyI GRCGYC 2 cut(s) 962, 1176
AfaI GTAC 4 cut(s) 90, 397, 428, 1264
AfeI AGCGCT 1 cut(s) 784
AfiI CCNNNNNNNGG 6 cut(s) 275, 343, 386, 579, 1046, 1400
AflIII ACRYGT 2 cut(s) 73, 1025
AgsI TTSAA 6 cut(s) 243, 285, 543, 1004, 1143, 1348
AjiI CACGTC 2 cut(s) 1028, 1118
AjnI CCWGG 1 cut(s) 229
AjuI GAANNNNNNNTTGG 1 cut(s) 1425
AloI GAACNNNNNNTCC 2 cut(s) 1046, 1078
AluBI AGCT 5 cut(s) 569, 847, 1045, 1062, 1316
AluI AGCT 5 cut(s) 569, 847, 1045, 1062, 1316
Alw21I GWGCWC 2 cut(s) 1064, 1408
Alw26I GTCTC 2 cut(s) 1110, 1212
Alw44I GTGCAC 1 cut(s) 1404
AlwI GGATC 6 cut(s) 395, 397, 606, 667, 785, 1178
AlwNI CAGNNNCTG 1 cut(s) 215
Ama87I CYCGRG 2 cut(s) 456, 1415
Aor51HI AGCGCT 1 cut(s) 784
AoxI GGCC 3 cut(s) 297, 1321, 1378
ApaLI GTGCAC 1 cut(s) 1404
ApeKI GCWGC 2 cut(s) 569, 1238
ApoI RAATTY 2 cut(s) 238, 1343
ArsI GACNNNNNNTTYG 4 cut(s) 1161, 1193, 1380, 1412
Asp718I GGTACC 1 cut(s) 1262
AspLEI GCGC 5 cut(s) 455, 785, 964, 1294, 1296
AspS9I GGNCC 1 cut(s) 1321
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 2 cut(s) 456, 1415
BaeGI GKGCMC 1 cut(s) 1408
BalI TGGCCA 2 cut(s) 299, 1380
BanI GGYRCC 2 cut(s) 961, 1262
BanII GRGCYC 1 cut(s) 1064
BarI GAAGNNNNNNTAC 2 cut(s) 843, 875
Bbv12I GWGCWC 2 cut(s) 1064, 1408
BbvI GCAGC 2 cut(s) 556, 1225
BccI CCATC 4 cut(s) 116, 542, 628, 670
BciT130I CCWGG 1 cut(s) 231
BciVI GTATCC 1 cut(s) 637
BclI TGATCA 1 cut(s) 685
BcoDI GTCTC 2 cut(s) 1110, 1212
BfaI CTAG 4 cut(s) 249, 516, 848, 1215
BfmI CTRYAG 2 cut(s) 1308, 1365
BfoI RGCGCY 2 cut(s) 786, 965
BfuI GTATCC 1 cut(s) 637
BisI GCNGC 3 cut(s) 570, 960, 1239
BlsI GCNGC 3 cut(s) 571, 961, 1240
Bme1390I CCNGG 1 cut(s) 231
BmeT110I CYCGRG 2 cut(s) 456, 1415
BmgBI CACGTC 2 cut(s) 1028, 1118
BmgT120I GGNCC 1 cut(s) 1321
BmiI GGNNCC 3 cut(s) 483, 963, 1264
BmrFI CCNGG 1 cut(s) 231
BmrI ACTGGG 1 cut(s) 425
BmsI GCATC 3 cut(s) 602, 727, 1342
BmuI ACTGGG 1 cut(s) 425
Bpu10I CCTNAGC 2 cut(s) 867, 1317
BpuEI CTTGAG 1 cut(s) 992
BsaHI GRCGYC 2 cut(s) 962, 1176
BsaJI CCNNGG 2 cut(s) 300, 1399
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 1046, 1076
Bsc4I CCNNNNNNNGG 6 cut(s) 275, 343, 386, 579, 1046, 1400
Bse1I ACTGG 5 cut(s) 300, 431, 565, 1303, 1394
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 300, 1399
BseGI GGATG 3 cut(s) 52, 553, 647
BseLI CCNNNNNNNGG 6 cut(s) 275, 343, 386, 579, 1046, 1400
BseMII CTCAG 3 cut(s) 377, 1241, 1331
BseNI ACTGG 5 cut(s) 300, 431, 565, 1303, 1394
BsePI GCGCGC 1 cut(s) 1292
BseRI GAGGAG 1 cut(s) 981
BseSI GKGCMC 1 cut(s) 1408
BseXI GCAGC 2 cut(s) 556, 1225
BseYI CCCAGC 3 cut(s) 379, 602, 1097
Bsh1236I CGCG 2 cut(s) 453, 1294
BshFI GGCC 3 cut(s) 299, 1323, 1380
BshNI GGYRCC 2 cut(s) 961, 1262
BsiHKAI GWGCWC 2 cut(s) 1064, 1408
BsiHKCI CYCGRG 2 cut(s) 456, 1415
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 6 cut(s) 275, 343, 386, 579, 1046, 1400
BsmAI GTCTC 2 cut(s) 1110, 1212
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 3 cut(s) 299, 1323, 1380
BsoBI CYCGRG 2 cut(s) 456, 1415
Bsp1286I GDGCHC 2 cut(s) 1064, 1408
BspACI CCGC 4 cut(s) 143, 651, 772, 959
BspANI GGCC 3 cut(s) 299, 1323, 1380
BspCNI CTCAG 3 cut(s) 376, 1240, 1330
BspFNI CGCG 2 cut(s) 453, 1294
BspLI GGNNCC 3 cut(s) 483, 963, 1264
BspMAI CTGCAG 2 cut(s) 1312, 1369
BspPI GGATC 6 cut(s) 395, 397, 606, 667, 785, 1178
BspQI GCTCTTC 1 cut(s) 935
BspT107I GGYRCC 2 cut(s) 961, 1262
BsrI ACTGG 5 cut(s) 300, 431, 565, 1303, 1394
BssECI CCNNGG 2 cut(s) 300, 1399
BssHII GCGCGC 1 cut(s) 1292
BssNI GRCGYC 2 cut(s) 962, 1176
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 3 cut(s) 196, 846, 935
BstACI GRCGYC 2 cut(s) 962, 1176
BstC8I GCNNGC 4 cut(s) 522, 894, 1294, 1365
BstDEI CTNAG 6 cut(s) 363, 867, 935, 1109, 1227, 1317
BstF5I GGATG 3 cut(s) 52, 553, 647
BstFNI CGCG 2 cut(s) 453, 1294
BstH2I RGCGCY 2 cut(s) 786, 965
BstHHI GCGC 5 cut(s) 455, 785, 964, 1294, 1296
BstMAI GTCTC 2 cut(s) 1110, 1212
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 229
BstSFI CTRYAG 2 cut(s) 1308, 1365
BstSLI GKGCMC 1 cut(s) 1408
BstUI CGCG 2 cut(s) 453, 1294
BstV1I GCAGC 2 cut(s) 556, 1225
BstX2I RGATCY 4 cut(s) 389, 400, 611, 1183
BstYI RGATCY 4 cut(s) 389, 400, 611, 1183
BsuI GTATCC 1 cut(s) 637
BsuRI GGCC 3 cut(s) 299, 1323, 1380
BtrI CACGTC 2 cut(s) 1028, 1118
BtsCI GGATG 3 cut(s) 52, 553, 647
BtsI GCAGTG 2 cut(s) 213, 1374
BtsIMutI CAGTG 4 cut(s) 213, 438, 1296, 1374
Cac8I GCNNGC 4 cut(s) 522, 894, 1294, 1365
CaiI CAGNNNCTG 1 cut(s) 215
CfoI GCGC 5 cut(s) 455, 785, 964, 1294, 1296
Cfr13I GGNCC 1 cut(s) 1321
CseI GACGC 1 cut(s) 332
Csp6I GTAC 4 cut(s) 89, 396, 427, 1263
CspCI CAANNNNNGTGG 2 cut(s) 544, 579
CviAII CATG 5 cut(s) 74, 263, 967, 1423, 1430
CviQI GTAC 4 cut(s) 89, 396, 427, 1263
DdeI CTNAG 6 cut(s) 363, 867, 935, 1109, 1227, 1317
DinI GGCGCC 1 cut(s) 963
DraI TTTAAA 1 cut(s) 696
EaeI YGGCCR 2 cut(s) 297, 1378
Eam1104I CTCTTC 3 cut(s) 196, 846, 935
EarI CTCTTC 3 cut(s) 196, 846, 935
EciI GGCGGA 2 cut(s) 640, 787
Ecl136II GAGCTC 1 cut(s) 1062
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 1064
Eco47III AGCGCT 1 cut(s) 784
Eco53kI GAGCTC 1 cut(s) 1062
Eco57I CTGAAG 3 cut(s) 216, 784, 1071
Eco88I CYCGRG 2 cut(s) 456, 1415
EcoICRI GAGCTC 1 cut(s) 1062
EcoRI GAATTC 1 cut(s) 1343
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 1064
EgeI GGCGCC 1 cut(s) 963
EheI GGCGCC 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 5 cut(s) 77, 266, 970, 1426, 1433
FaiI YATR 9 cut(s) 75, 131, 264, 407, 968, 981, 1181, 1424, 1431
FatI CATG 5 cut(s) 73, 262, 966, 1422, 1429
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 3 cut(s) 570, 960, 1239
FokI GGATG 3 cut(s) 59, 560, 634
FriOI GRGCYC 1 cut(s) 1064
Fsp4HI GCNGC 3 cut(s) 570, 960, 1239
FspBI CTAG 4 cut(s) 249, 516, 848, 1215
GlaI GCGC 5 cut(s) 454, 784, 963, 1293, 1295
GluI GCNGC 3 cut(s) 570, 960, 1239
GsaI CCCAGC 3 cut(s) 383, 606, 1101
HaeII RGCGCY 2 cut(s) 786, 965
HaeIII GGCC 3 cut(s) 299, 1323, 1380
HapII CCGG 1 cut(s) 92
HgaI GACGC 1 cut(s) 332
HhaI GCGC 5 cut(s) 455, 785, 964, 1294, 1296
Hin1I GRCGYC 2 cut(s) 962, 1176
Hin1II CATG 5 cut(s) 77, 266, 970, 1426, 1433
Hin6I GCGC 5 cut(s) 453, 783, 962, 1292, 1294
HinP1I GCGC 5 cut(s) 453, 783, 962, 1292, 1294
HindIII AAGCTT 1 cut(s) 1043
HinfI GANTC 6 cut(s) 20, 256, 367, 910, 1015, 1123
HpaII CCGG 1 cut(s) 92
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 3 cut(s) 148, 427, 1406
Hpy188III TCNNGA 6 cut(s) 789, 914, 1009, 1127, 1229, 1340
Hpy8I GTNNAC 3 cut(s) 148, 427, 1406
HpyAV CCTTC 4 cut(s) 173, 808, 823, 1044
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 4 cut(s) 814, 1027, 1117, 1176
HpyCH4V TGCA 4 cut(s) 119, 1310, 1367, 1406
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 6 cut(s) 363, 867, 935, 1109, 1227, 1317
HpySE526I ACGT 4 cut(s) 814, 1027, 1117, 1176
Hsp92I GRCGYC 2 cut(s) 962, 1176
Hsp92II CATG 5 cut(s) 77, 266, 970, 1426, 1433
HspAI GCGC 5 cut(s) 453, 783, 962, 1292, 1294
KasI GGCGCC 1 cut(s) 961
KpnI GGTACC 1 cut(s) 1266
Ksp22I TGATCA 1 cut(s) 685
LguI GCTCTTC 1 cut(s) 935
LmnI GCTCC 4 cut(s) 271, 487, 566, 1067
Lsp1109I GCAGC 2 cut(s) 556, 1225
LweI GCATC 3 cut(s) 602, 727, 1342
MaeI CTAG 4 cut(s) 249, 516, 848, 1215
MaeII ACGT 4 cut(s) 814, 1027, 1117, 1176
MaeIII GTNAC 2 cut(s) 166, 205
MflI RGATCY 4 cut(s) 389, 400, 611, 1183
MhlI GDGCHC 2 cut(s) 1064, 1408
MlsI TGGCCA 2 cut(s) 299, 1380
MluCI AATT 4 cut(s) 238, 1081, 1138, 1343
MluNI TGGCCA 2 cut(s) 299, 1380
Mly113I GGCGCC 1 cut(s) 962
MlyI GAGTC 3 cut(s) 29, 376, 919
MmeI TCCRAC 3 cut(s) 536, 786, 1423
Mox20I TGGCCA 2 cut(s) 299, 1380
MscI TGGCCA 2 cut(s) 299, 1380
MseI TTAA 8 cut(s) 186, 447, 576, 695, 954, 1154, 1200, 1450
MslI CAYNNNNRTG 2 cut(s) 78, 422
Msp20I TGGCCA 2 cut(s) 299, 1380
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 2 cut(s) 453, 1294
MwoI GCNNNNNNNGC 1 cut(s) 108
NarI GGCGCC 1 cut(s) 962
NlaIII CATG 5 cut(s) 77, 266, 970, 1426, 1433
NlaIV GGNNCC 3 cut(s) 483, 963, 1264
NmuCI GTSAC 2 cut(s) 166, 205
NspI RCATGY 1 cut(s) 77
PaeR7I CTCGAG 1 cut(s) 456
PauI GCGCGC 1 cut(s) 1292
PciI ACATGT 1 cut(s) 73
PciSI GCTCTTC 1 cut(s) 935
PcsI WCGNNNNNNNCGW 1 cut(s) 1123
PctI GAATGC 1 cut(s) 121
PfeI GAWTC 3 cut(s) 256, 1015, 1123
PkrI GCNGC 3 cut(s) 571, 961, 1240
PleI GAGTC 3 cut(s) 28, 375, 918
PluTI GGCGCC 1 cut(s) 965
PpsI GAGTC 3 cut(s) 28, 375, 918
PscI ACATGT 1 cut(s) 73
Psp124BI GAGCTC 1 cut(s) 1064
Psp6I CCWGG 1 cut(s) 229
PspFI CCCAGC 3 cut(s) 379, 602, 1097
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 3 cut(s) 483, 963, 1264
PspPI GGNCC 1 cut(s) 1321
PspXI VCTCGAGB 1 cut(s) 456
PstI CTGCAG 2 cut(s) 1312, 1369
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 4 cut(s) 389, 400, 611, 1183
PteI GCGCGC 1 cut(s) 1292
RsaI GTAC 4 cut(s) 90, 397, 428, 1264
RsaNI GTAC 4 cut(s) 89, 396, 427, 1263
RseI CAYNNNNRTG 2 cut(s) 78, 422
SacI GAGCTC 1 cut(s) 1064
SapI GCTCTTC 1 cut(s) 935
SaqAI TTAA 8 cut(s) 186, 447, 576, 695, 954, 1154, 1200, 1450
SatI GCNGC 3 cut(s) 570, 960, 1239
Sau96I GGNCC 1 cut(s) 1321
SchI GAGTC 3 cut(s) 29, 376, 919
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 2 cut(s) 1064, 1408
SfaNI GCATC 3 cut(s) 602, 727, 1342
SfcI CTRYAG 2 cut(s) 1308, 1365
SfoI GGCGCC 1 cut(s) 963
Sfr274I CTCGAG 1 cut(s) 456
SlaI CTCGAG 1 cut(s) 456
SmiMI CAYNNNNRTG 2 cut(s) 78, 422
SmlI CTYRAG 2 cut(s) 456, 1007
SmoI CTYRAG 2 cut(s) 456, 1007
Sse9I AATT 4 cut(s) 238, 1081, 1138, 1343
SsiI CCGC 4 cut(s) 143, 651, 772, 959
SspDI GGCGCC 1 cut(s) 961
SspMI CTAG 4 cut(s) 249, 516, 848, 1215
SstI GAGCTC 1 cut(s) 1064
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 4 cut(s) 817, 1030, 1120, 1179
TaqI TCGA 3 cut(s) 457, 1126, 1386
TasI AATT 4 cut(s) 238, 1081, 1138, 1343
TauI GCSGC 1 cut(s) 962
TfiI GAWTC 3 cut(s) 256, 1015, 1123
Tru1I TTAA 8 cut(s) 186, 447, 576, 695, 954, 1154, 1200, 1450
Tru9I TTAA 8 cut(s) 186, 447, 576, 695, 954, 1154, 1200, 1450
TscAI CASTG 4 cut(s) 220, 438, 1303, 1374
TseFI GTSAC 2 cut(s) 166, 205
TseI GCWGC 2 cut(s) 569, 1238
Tsp45I GTSAC 2 cut(s) 166, 205
TspDTI ATGAA 6 cut(s) 192, 251, 422, 527, 1211, 1446
TspGWI ACGGA 3 cut(s) 165, 736, 841
TspRI CASTG 4 cut(s) 220, 438, 1303, 1374
VneI GTGCAC 1 cut(s) 1404
XapI RAATTY 2 cut(s) 238, 1343
XceI RCATGY 1 cut(s) 77
XhoI CTCGAG 1 cut(s) 456
XspI CTAG 4 cut(s) 249, 516, 848, 1215
ZraI GACGTC 1 cut(s) 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.