RLG00000023738

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0006819
Physical Location & Seq
Reverse (-)
27669587 .. 27671927
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023738

Sequence Viewer

Length: 1398 bp
ATGGAGTCTGAAAATGGGATTGCTTTAGGGGATGAGAATTGTGCTGTTGTGGAAAAACATGTAGAAGAATCAGTACTGGATTTGAACATTGAAGGAAAGAATGTTGATGCTAATGTAGAAGTTCCTACCATGAATGGAGTATCTGAACCTGTAAGCAAAGATGAAGGCATTAAACCTTCAGGAGGTGCAGTTGAAGTTGCTGCAAGTGTTCCGCCTGGTAAAAATTCAAAAACAAAAAAGGATCCTCATGCTCCAAACAATGGTGTTTCAAAGGGAAAGTTGGCCAAGGATAAACCCAGTGTGAAAGGTGCAACTCCAGTTTCTCGTACACAAAGGGCAATACTTTCTCAGAGTCTTTCGTTCCCAGCCAGGGGAGCTCGTGTTGATGCTATGAAGAAGAGCATTGACGTTTATCCAGTGAAAACAGAAGTCAAACACACTCGAGGGAATGCAACCAAAGCTGAGGCCCCTTTTCCATCTTCTCGTTTGAATCATCAAAATAGGCGTGCATCCACAGGAATACACTCAAATGATGAGAACAAAGCTGGTGGAGCTTCAGTTAGGAGGACTACTTTAGCATCAACACCTAGCATTAGATCCTCTGCATCAATGAAGTCTGGCTCTGTGAGTAAACCTGCTAATAGTTCATATGATGTGACCCGATCAGTTGGGCAGATCTTAAGTGCTGTAAAAACAACCCTGCCAATCAAAGAGGATGATGATGCTCATTCCATTGCTTCAAGCACTACACCTGGTGGGCGGAGGAGCAGTGGTTCTGCATTTTCCTTCAGGTTGGATGAACGTGCTGAAAAAAGGAAGGAGTTCTTTACAAAGCTAGAAGAGAAGATACAGGCTAAGGAAGCAGAAAAAAATAATTCGCAAGCAAAATCAAAGATACCGACCACACGTCCCATATCTCCAAAGCTTGGAAGGCACAAGAACTCCATTTCTTCATTGAACAACCCCTCAGAAGGTGGTGGGACGTGTCTTAGCCCGCGTCTGAATCGAGAACAGAACAATTCAAACAAAGGGTTAAAGGCAACAGGTGAAAAAGATGTCACAGAATCAAAGAAGCCTATCAGGAAGTCCCAACATCGACTTCATTCTCAGGAAAATGTGGCTGCTAAAGCTGAAGGAAAGCCCTGTAAGTCACAACTGAAAGTGGTCAGCAAAGAAGAAAAAGAAAGCCCGAAGGCATGCACTGGAGAGACAGAAGAAAGCTTCCAAGACCAATCTGAACATCTTTCTGAATGCAAAGATAAGATAGAGCCTGAAGTGAGTGTTGACCAGACCAAAGAACCAGTTGTCAGTGCAGCTCCTGCTCCTGAGATCATGCCTCAAGAAGTTACCATAGCAGCTCCTGAGATCATGCCTCGAGGAGTTACCATTGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

466

Amino Acids

49.71

Weight (kDa)

9.31

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 261 - 299 2.2e-10 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1374
Acc36I ACCTGC 1 cut(s) 643
AccB7I CCANNNNNTGG 2 cut(s) 260, 926
AccII CGCG 1 cut(s) 997
AciI CCGC 3 cut(s) 212, 760, 995
AclWI GGATC 3 cut(s) 236, 249, 591
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 5 cut(s) 162, 540, 772, 1152, 1293
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 2 cut(s) 75, 328
AfiI CCNNNNNNNGG 6 cut(s) 182, 260, 370, 371, 926, 971
AflII CTTAAG 1 cut(s) 679
AflIII ACRYGT 3 cut(s) 58, 905, 983
AgsI TTSAA 9 cut(s) 85, 92, 194, 228, 270, 490, 741, 958, 1023
AhdI GACNNNNNGTC 1 cut(s) 906
AjiI CACGTC 2 cut(s) 908, 984
AjnI CCWGG 3 cut(s) 214, 368, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 1083, 1115
AloI GAACNNNNNNTCC 2 cut(s) 757, 789
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 1 cut(s) 1202
AlwI GGATC 3 cut(s) 236, 249, 591
AlwNI CAGNNNCTG 2 cut(s) 1319, 1361
Ama87I CYCGRG 2 cut(s) 441, 1374
AoxI GGCC 2 cut(s) 282, 465
ApeKI GCWGC 4 cut(s) 200, 1121, 1313, 1355
ApoI RAATTY 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 821
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 1058
AvaI CYCGRG 2 cut(s) 441, 1374
BalI TGGCCA 1 cut(s) 284
BamHI GGATCC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 379
BarI GAAGNNNNNNTAC 4 cut(s) 57, 89, 831, 863
BauI CACGAG 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 379
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 4 cut(s) 187, 1108, 1325, 1367
BccI CCATC 1 cut(s) 484
BciT130I CCWGG 3 cut(s) 216, 370, 753
BcoDI GTCTC 1 cut(s) 1202
BfaI CTAG 2 cut(s) 588, 836
BfrI CTTAAG 1 cut(s) 679
BfuAI ACCTGC 1 cut(s) 643
BglII AGATCT 1 cut(s) 675
BisI GCNGC 4 cut(s) 201, 1122, 1314, 1356
BlsI GCNGC 4 cut(s) 202, 1123, 1315, 1357
BmcAI AGTACT 1 cut(s) 75
Bme1390I CCNGG 3 cut(s) 216, 370, 753
BmeRI GACNNNNNGTC 1 cut(s) 906
BmeT110I CYCGRG 2 cut(s) 441, 1374
BmgBI CACGTC 2 cut(s) 908, 984
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 2 cut(s) 243, 468
BmrFI CCNGG 3 cut(s) 216, 370, 753
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
BmuI ACTGGG 1 cut(s) 291
BpmI CTGGAG 2 cut(s) 300, 1224
Bpu10I CCTNAGC 2 cut(s) 462, 855
BpuEI CTTGAG 1 cut(s) 1323
BsaJI CCNNGG 2 cut(s) 285, 369
Bsc4I CCNNNNNNNGG 6 cut(s) 182, 260, 370, 371, 926, 971
Bse1I ACTGG 6 cut(s) 81, 297, 317, 416, 1207, 1301
Bse3DI GCAATG 1 cut(s) 732
BseBI CCWGG 3 cut(s) 216, 370, 753
BseDI CCNNGG 2 cut(s) 285, 369
BseGI GGATG 4 cut(s) 37, 509, 721, 802
BseLI CCNNNNNNNGG 6 cut(s) 182, 260, 370, 371, 926, 971
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 6 cut(s) 362, 453, 981, 1121, 1317, 1353
BseNI ACTGG 6 cut(s) 81, 297, 317, 416, 1207, 1301
BseRI GAGGAG 2 cut(s) 778, 1392
BseXI GCAGC 4 cut(s) 187, 1108, 1325, 1367
BseYI CCCAGC 1 cut(s) 364
BsgI GTGCAG 2 cut(s) 207, 1332
Bsh1236I CGCG 1 cut(s) 997
BshFI GGCC 2 cut(s) 284, 467
BsiHKAI GWGCWC 1 cut(s) 379
BsiHKCI CYCGRG 2 cut(s) 441, 1374
BslFI GGGAC 3 cut(s) 894, 994, 1072
BslI CCNNNNNNNGG 6 cut(s) 182, 260, 370, 371, 926, 971
BsmAI GTCTC 1 cut(s) 1202
BsmFI GGGAC 3 cut(s) 894, 994, 1072
BsmI GAATGC 2 cut(s) 454, 1256
BsnI GGCC 2 cut(s) 284, 467
BsoBI CYCGRG 2 cut(s) 441, 1374
Bsp1286I GDGCHC 1 cut(s) 379
Bsp143I GATC 6 cut(s) 241, 596, 662, 675, 1329, 1365
BspACI CCGC 3 cut(s) 212, 760, 995
BspANI GGCC 2 cut(s) 284, 467
BspCNI CTCAG 6 cut(s) 361, 454, 980, 1120, 1318, 1354
BspFNI CGCG 1 cut(s) 997
BspLI GGNNCC 2 cut(s) 243, 468
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 3 cut(s) 236, 249, 591
BspQI GCTCTTC 1 cut(s) 392
BspTI CTTAAG 1 cut(s) 679
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 6 cut(s) 81, 297, 317, 416, 1207, 1301
BssECI CCNNGG 2 cut(s) 285, 369
BssMI GATC 6 cut(s) 241, 596, 662, 675, 1329, 1365
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 378
Bst2UI CCWGG 3 cut(s) 216, 370, 753
Bst6I CTCTTC 2 cut(s) 392, 834
BstAFI CTTAAG 1 cut(s) 679
BstAPI GCANNNNNTGC 1 cut(s) 1319
BstC8I GCNNGC 4 cut(s) 507, 882, 995, 1198
BstDEI CTNAG 8 cut(s) 348, 462, 855, 967, 989, 1107, 1326, 1362
BstENI CCTNNNNNAGG 1 cut(s) 180
BstF5I GGATG 4 cut(s) 37, 509, 721, 802
BstFNI CGCG 1 cut(s) 997
BstKTI GATC 6 cut(s) 244, 599, 665, 678, 1332, 1368
BstMAI GTCTC 1 cut(s) 1202
BstMBI GATC 6 cut(s) 241, 596, 662, 675, 1329, 1365
BstMWI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 860, 931, 1127, 1319
BstNI CCWGG 3 cut(s) 216, 370, 753
BstNSI RCATGY 2 cut(s) 62, 1200
BstSCI CCNGG 3 cut(s) 214, 368, 751
BstUI CGCG 1 cut(s) 997
BstV1I GCAGC 4 cut(s) 187, 1108, 1325, 1367
BstX2I RGATCY 3 cut(s) 241, 596, 675
BstYI RGATCY 3 cut(s) 241, 596, 675
BsuRI GGCC 2 cut(s) 284, 467
BtrI CACGTC 2 cut(s) 908, 984
BtsCI GGATG 4 cut(s) 37, 509, 721, 802
BtsI GCAGTG 1 cut(s) 775
BtsIMutI CAGTG 5 cut(s) 304, 423, 775, 1200, 1315
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 4 cut(s) 507, 882, 995, 1198
CaiI CAGNNNCTG 2 cut(s) 1319, 1361
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 1 cut(s) 986
CsiI ACCWGGT 1 cut(s) 751
Csp6I GTAC 2 cut(s) 74, 327
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviAII CATG 6 cut(s) 59, 130, 248, 1197, 1333, 1369
CviQI GTAC 2 cut(s) 74, 327
DdeI CTNAG 8 cut(s) 348, 462, 855, 967, 989, 1107, 1326, 1362
DpnI GATC 6 cut(s) 243, 598, 664, 677, 1331, 1367
DpnII GATC 6 cut(s) 241, 596, 662, 675, 1329, 1365
DraIII CACNNNGTG 1 cut(s) 755
DriI GACNNNNNGTC 1 cut(s) 906
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 2 cut(s) 392, 834
Eam1105I GACNNNNNGTC 1 cut(s) 906
EarI CTCTTC 2 cut(s) 392, 834
EciI GGCGGA 2 cut(s) 201, 775
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 377
Eco57I CTGAAG 5 cut(s) 162, 540, 772, 1152, 1293
Eco88I CYCGRG 2 cut(s) 441, 1374
EcoICRI GAGCTC 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 3 cut(s) 214, 368, 751
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 6 cut(s) 62, 133, 251, 1200, 1336, 1372
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 3 cut(s) 894, 994, 1072
FatI CATG 6 cut(s) 58, 129, 247, 1196, 1332, 1368
FauI CCCGC 1 cut(s) 1002
FauNDI CATATG 1 cut(s) 649
Fnu4HI GCNGC 4 cut(s) 201, 1122, 1314, 1356
FokI GGATG 4 cut(s) 44, 496, 728, 809
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 4 cut(s) 201, 1122, 1314, 1356
FspBI CTAG 2 cut(s) 588, 836
GluI GCNGC 4 cut(s) 201, 1122, 1314, 1356
GsaI CCCAGC 1 cut(s) 368
GsuI CTGGAG 2 cut(s) 300, 1224
HaeIII GGCC 2 cut(s) 284, 467
HgaI GACGC 1 cut(s) 986
Hin1II CATG 6 cut(s) 62, 133, 251, 1200, 1336, 1372
HincII GTYRAC 1 cut(s) 1285
HindII GTYRAC 1 cut(s) 1285
HindIII AAGCTT 2 cut(s) 923, 1219
HinfI GANTC 6 cut(s) 5, 68, 352, 490, 1003, 1064
HphI GGTGA 1 cut(s) 1058
Hpy166II GTNNAC 3 cut(s) 329, 632, 1285
Hpy188I TCNGA 7 cut(s) 10, 145, 351, 970, 1002, 1237, 1249
Hpy188III TCNNGA 7 cut(s) 180, 1007, 1081, 1109, 1325, 1340, 1361
Hpy8I GTNNAC 3 cut(s) 329, 632, 1285
HpyAV CCTTC 9 cut(s) 86, 158, 186, 796, 811, 924, 965, 1127, 1186
HpyCH4IV ACGT 4 cut(s) 408, 802, 907, 983
HpyF10VI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 860, 931, 1127, 1319
HpyF3I CTNAG 8 cut(s) 348, 462, 855, 967, 989, 1107, 1326, 1362
HpySE526I ACGT 4 cut(s) 408, 802, 907, 983
Hsp92II CATG 6 cut(s) 62, 133, 251, 1200, 1336, 1372
Kzo9I GATC 6 cut(s) 241, 596, 662, 675, 1329, 1365
LguI GCTCTTC 1 cut(s) 392
LmnI GCTCC 7 cut(s) 256, 374, 551, 765, 1321, 1327, 1363
Lsp1109I GCAGC 4 cut(s) 187, 1108, 1325, 1367
LweI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
MabI ACCWGGT 1 cut(s) 751
MaeI CTAG 2 cut(s) 588, 836
MaeII ACGT 4 cut(s) 408, 802, 907, 983
MaeIII GTNAC 5 cut(s) 655, 1057, 1149, 1345, 1381
MalI GATC 6 cut(s) 243, 598, 664, 677, 1331, 1367
MboI GATC 6 cut(s) 241, 596, 662, 675, 1329, 1365
MboII GAAGA 9 cut(s) 77, 406, 409, 471, 851, 856, 942, 1187, 1226
MflI RGATCY 3 cut(s) 241, 596, 675
MhlI GDGCHC 1 cut(s) 379
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 4 cut(s) 37, 223, 874, 1018
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 2 cut(s) 14, 361
MmeI TCCRAC 1 cut(s) 774
Mox20I TGGCCA 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 821
MscI TGGCCA 1 cut(s) 284
MseI TTAA 4 cut(s) 171, 680, 1034, 1396
MslI CAYNNNNRTG 1 cut(s) 528
Msp20I TGGCCA 1 cut(s) 284
MspCI CTTAAG 1 cut(s) 679
MspR9I CCNGG 3 cut(s) 216, 370, 753
Mva1269I GAATGC 2 cut(s) 454, 1256
MvaI CCWGG 3 cut(s) 216, 370, 753
MvnI CGCG 1 cut(s) 997
MwoI GCNNNNNNNGC 7 cut(s) 374, 458, 551, 860, 931, 1127, 1319
NdeI CATATG 1 cut(s) 649
NdeII GATC 6 cut(s) 241, 596, 662, 675, 1329, 1365
NlaIII CATG 6 cut(s) 62, 133, 251, 1200, 1336, 1372
NlaIV GGNNCC 2 cut(s) 243, 468
NmuCI GTSAC 3 cut(s) 655, 1057, 1149
NspI RCATGY 2 cut(s) 62, 1200
PaeI GCATGC 1 cut(s) 1200
PaeR7I CTCGAG 2 cut(s) 441, 1374
PciI ACATGT 1 cut(s) 58
PciSI GCTCTTC 1 cut(s) 392
PctI GAATGC 2 cut(s) 454, 1256
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 4 cut(s) 68, 490, 1003, 1064
PflMI CCANNNNNTGG 2 cut(s) 260, 926
PkrI GCNGC 4 cut(s) 202, 1123, 1315, 1357
PleI GAGTC 2 cut(s) 13, 360
PpsI GAGTC 2 cut(s) 13, 360
PscI ACATGT 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 214, 368, 751
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 3 cut(s) 214, 368, 751
PspN4I GGNNCC 2 cut(s) 243, 468
PspPI GGNCC 1 cut(s) 466
PspXI VCTCGAGB 2 cut(s) 441, 1374
PstNI CAGNNNCTG 2 cut(s) 1319, 1361
PsuI RGATCY 3 cut(s) 241, 596, 675
RsaI GTAC 2 cut(s) 75, 328
RsaNI GTAC 2 cut(s) 74, 327
RseI CAYNNNNRTG 1 cut(s) 528
SacI GAGCTC 1 cut(s) 379
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 4 cut(s) 171, 680, 1034, 1396
SatI GCNGC 4 cut(s) 201, 1122, 1314, 1356
Sau3AI GATC 6 cut(s) 241, 596, 662, 675, 1329, 1365
Sau96I GGNCC 1 cut(s) 466
ScaI AGTACT 1 cut(s) 75
SchI GAGTC 2 cut(s) 14, 361
ScrFI CCNGG 3 cut(s) 216, 370, 753
SduI GDGCHC 1 cut(s) 379
SexAI ACCWGGT 1 cut(s) 751
SfaNI GCATC 6 cut(s) 97, 376, 518, 587, 614, 712
Sfr274I CTCGAG 2 cut(s) 441, 1374
SlaI CTCGAG 2 cut(s) 441, 1374
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 4 cut(s) 441, 679, 1338, 1374
SmoI CTYRAG 4 cut(s) 441, 679, 1338, 1374
SphI GCATGC 1 cut(s) 1200
Sse9I AATT 4 cut(s) 37, 223, 874, 1018
SsiI CCGC 3 cut(s) 212, 760, 995
SspMI CTAG 2 cut(s) 588, 836
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 3 cut(s) 214, 368, 751
StyI CCWWGG 1 cut(s) 285
TaiI ACGT 4 cut(s) 411, 805, 910, 986
TaqI TCGA 4 cut(s) 442, 1006, 1096, 1375
TasI AATT 4 cut(s) 37, 223, 874, 1018
TatI WGTACW 1 cut(s) 73
TfiI GAWTC 4 cut(s) 68, 490, 1003, 1064
Tru1I TTAA 4 cut(s) 171, 680, 1034, 1396
Tru9I TTAA 4 cut(s) 171, 680, 1034, 1396
TscAI CASTG 5 cut(s) 304, 423, 775, 1207, 1315
TseFI GTSAC 3 cut(s) 655, 1057, 1149
TseI GCWGC 4 cut(s) 200, 1121, 1313, 1355
Tsp45I GTSAC 3 cut(s) 655, 1057, 1149
TspDTI ATGAA 8 cut(s) 146, 177, 407, 626, 636, 813, 942, 1091
TspRI CASTG 5 cut(s) 304, 423, 775, 1207, 1315
Van91I CCANNNNNTGG 2 cut(s) 260, 926
Vha464I CTTAAG 1 cut(s) 679
XagI CCTNNNNNAGG 1 cut(s) 180
XapI RAATTY 1 cut(s) 223
XceI RCATGY 2 cut(s) 62, 1200
XhoI CTCGAG 2 cut(s) 441, 1374
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 2 cut(s) 588, 836
ZrmI AGTACT 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.