AT2G38550

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009460
Physical Location & Seq
Forward (+)
16131730 .. 16134429
2700 bp
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UTR
Exon/CDS
Intron
AT2G38550.1

Sequence Viewer

Length: 1008 bp
ATGATGAGTATCCCAATGGAGTTAATGTCTATCAGAAACCCTAATTCCACCTTGCTCTATAGAGCTCACTCTCGTCCGCCTGTGAAGCTCTGTGCTCCACCTCGTTCTCTCCTCCCTAGCCGACGCCATTTCAGTGCTCCTCGAGCTGTTGTTTCGTATCCAGGAATACGTTTCGGCTTTACCTCGCCGGAAGTCTTGCTTAATCGATCCGTCGTTGCTTTCGCTGCATCCCACGAGGATTCGGGAGAATCAGGTGTTGAGGTGGGGAAGGAGAAGAGTGATATAGATGTTGAAGATGATACATCTAAGGAAGCATGGAAACAAACTCTTGAGTCTTTTAAAGAGCAGGTATCAAAGATGCAGAGTGTGTCATCGGAGGCATACTCCGTTAACTCCCAAAAGGCGATGACGGTCTTGAAAGAAACTTCTGAACAACTTAGGATTCAAGCGGAAAAGGCGAAAGAAGAGTTGGGTACTAAAGCGAAAGTGGTTAGTGAAGAGGGTAGAGAATATATTTTGAAAGCAGCAGAAGAATCTCCATCAGATGTGAAAGAGATTGTTGAAGCATTTGCCTCCACTGAGGATCTGAAAAATGTGTCCCGAGCGAATGATTTTCATGTTGGAATACCTTATGGTCTACTCTTGCTTGTGGGTGGTTTTATTAACTTCATGGTATCGGGAAGCATTCCGGCCATCAGGTTTGGAGTCATTCTTGGTGGAGCCCTTTTTGCACTGAGCCTGGCAAGTCTAAAGTCTCATAGGAAAGGAGAGTCATCTACAAAGTTCTTAAAAGGACAGATGGCTATAGTGGCAATCATATTTTTGAGAGAGTTGCGGTTGCTACTGTCTCAGAAATCGACGTTCCTAGGTTTTTTTACCACCCTTACAAGTGGTGGTGTGCTAGGGTTTTACTTGTACAAGATGGTGGTCAAGAGAGAAAAGGGACCAACCTTAGAAGATGGCGGAGAAGATGAATCAAGCGATGGGTTTGTAAGAAGTGAAGGATAA

Protein Analysis

335

Amino Acids

36.7

Weight (kDa)

7.0

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 207 - 304 1.7e-13 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 637
AciI CCGC 4 cut(s) 77, 449, 835, 963
AclWI GGATC 2 cut(s) 201, 591
AcoI YGGCCR 1 cut(s) 690
AcyI GRCGYC 1 cut(s) 124
AfaI GTAC 2 cut(s) 475, 917
AgsI TTSAA 5 cut(s) 293, 418, 446, 520, 563
AjnI CCWGG 2 cut(s) 160, 738
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AluBI AGCT 3 cut(s) 65, 88, 146
AluI AGCT 3 cut(s) 65, 88, 146
Alw21I GWGCWC 3 cut(s) 67, 97, 139
Alw26I GTCTC 2 cut(s) 759, 852
AlwI GGATC 2 cut(s) 201, 591
Ama87I CYCGRG 2 cut(s) 141, 600
AoxI GGCC 1 cut(s) 690
ApeKI GCWGC 2 cut(s) 224, 524
AspA2I CCTAGG 1 cut(s) 865
AspS9I GGNCC 1 cut(s) 944
AvaI CYCGRG 2 cut(s) 141, 600
AvaII GGWCC 1 cut(s) 944
AvrII CCTAGG 1 cut(s) 865
BanII GRGCYC 2 cut(s) 67, 724
BauI CACGAG 1 cut(s) 233
Bbv12I GWGCWC 3 cut(s) 67, 97, 139
BbvI GCAGC 2 cut(s) 211, 536
BccI CCATC 6 cut(s) 547, 701, 793, 916, 953, 977
BciT130I CCWGG 2 cut(s) 162, 740
BciVI GTATCC 2 cut(s) 20, 168
BcoDI GTCTC 2 cut(s) 759, 852
BfaI CTAG 3 cut(s) 117, 866, 902
BfmI CTRYAG 2 cut(s) 58, 804
BfuAI ACCTGC 1 cut(s) 337
BfuI GTATCC 2 cut(s) 20, 168
BisI GCNGC 2 cut(s) 225, 525
BlnI CCTAGG 1 cut(s) 865
BlsI GCNGC 2 cut(s) 226, 526
Bme1390I CCNGG 2 cut(s) 162, 740
Bme18I GGWCC 1 cut(s) 944
BmeT110I CYCGRG 2 cut(s) 141, 600
BmgT120I GGNCC 1 cut(s) 944
BmiI GGNNCC 2 cut(s) 721, 945
BmrFI CCNGG 2 cut(s) 162, 740
BmsI GCATC 2 cut(s) 236, 348
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpuEI CTTGAG 1 cut(s) 350
Bsa29I ATCGAT 1 cut(s) 205
BsaBI GATNNNNATC 1 cut(s) 8
BsaHI GRCGYC 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 865
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 2 cut(s) 162, 740
BseCI ATCGAT 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 865
BseGI GGATG 1 cut(s) 227
BseJI GATNNNNATC 1 cut(s) 8
BseMII CTCAG 3 cut(s) 570, 725, 863
BseRI GAGGAG 2 cut(s) 101, 129
BseXI GCAGC 2 cut(s) 211, 536
BshFI GGCC 1 cut(s) 692
BshVI ATCGAT 1 cut(s) 205
BsiHKAI GWGCWC 3 cut(s) 67, 97, 139
BsiHKCI CYCGRG 2 cut(s) 141, 600
BsiSI CCGG 2 cut(s) 188, 689
BslFI GGGAC 2 cut(s) 583, 957
BsmAI GTCTC 2 cut(s) 759, 852
BsmFI GGGAC 2 cut(s) 583, 957
BsmI GAATGC 1 cut(s) 684
BsnI GGCC 1 cut(s) 692
BsoBI CYCGRG 2 cut(s) 141, 600
Bsp1286I GDGCHC 4 cut(s) 67, 97, 139, 724
Bsp1407I TGTACA 1 cut(s) 915
Bsp143I GATC 2 cut(s) 206, 583
BspACI CCGC 4 cut(s) 77, 449, 835, 963
BspANI GGCC 1 cut(s) 692
BspCNI CTCAG 3 cut(s) 571, 726, 862
BspDI ATCGAT 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 721, 945
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 2 cut(s) 201, 591
BsrGI TGTACA 1 cut(s) 915
BssECI CCNNGG 1 cut(s) 865
BssMI GATC 2 cut(s) 206, 583
BssNI GRCGYC 1 cut(s) 124
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 865
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 2 cut(s) 162, 740
Bst4CI ACNGT 2 cut(s) 412, 846
Bst6I CTCTTC 3 cut(s) 269, 459, 492
BstACI GRCGYC 1 cut(s) 124
BstAUI TGTACA 1 cut(s) 915
BstDEI CTNAG 6 cut(s) 306, 437, 579, 734, 849, 952
BstF5I GGATG 1 cut(s) 227
BstKTI GATC 2 cut(s) 209, 586
BstMAI GTCTC 2 cut(s) 759, 852
BstMBI GATC 2 cut(s) 206, 583
BstMWI GCNNNNNNNGC 6 cut(s) 85, 143, 224, 455, 728, 809
BstNI CCWGG 2 cut(s) 162, 740
BstSCI CCNGG 2 cut(s) 160, 738
BstSFI CTRYAG 2 cut(s) 58, 804
BstV1I GCAGC 2 cut(s) 211, 536
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
Bsu15I ATCGAT 1 cut(s) 205
BsuI GTATCC 2 cut(s) 20, 168
BsuRI GGCC 1 cut(s) 692
BsuTUI ATCGAT 1 cut(s) 205
BtgZI GCGATG 2 cut(s) 419, 996
BtsCI GGATG 1 cut(s) 227
BtsIMutI CAGTG 3 cut(s) 139, 576, 731
BveI ACCTGC 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 944
ClaI ATCGAT 1 cut(s) 205
CseI GACGC 1 cut(s) 132
Csp6I GTAC 2 cut(s) 474, 916
CviAII CATG 3 cut(s) 315, 617, 670
CviJI RGCY 9 cut(s) 65, 88, 120, 146, 177, 692, 722, 738, 803
CviKI_1 RGCY 9 cut(s) 65, 88, 120, 146, 177, 692, 722, 738, 803
CviQI GTAC 2 cut(s) 474, 916
DdeI CTNAG 6 cut(s) 306, 437, 579, 734, 849, 952
DpnI GATC 2 cut(s) 208, 585
DpnII GATC 2 cut(s) 206, 583
DraI TTTAAA 1 cut(s) 340
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 3 cut(s) 269, 459, 492
EarI CTCTTC 3 cut(s) 269, 459, 492
EciI GGCGGA 2 cut(s) 66, 978
Ecl136II GAGCTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 865
Eco24I GRGCYC 2 cut(s) 67, 724
Eco47I GGWCC 1 cut(s) 944
Eco53kI GAGCTC 1 cut(s) 65
Eco88I CYCGRG 2 cut(s) 141, 600
EcoICRI GAGCTC 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 160, 738
EcoT14I CCWWGG 1 cut(s) 865
EcoT38I GRGCYC 2 cut(s) 67, 724
ErhI CCWWGG 1 cut(s) 865
FaeI CATG 3 cut(s) 318, 620, 673
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FaqI GGGAC 2 cut(s) 583, 957
FatI CATG 3 cut(s) 314, 616, 669
FblI GTMKAC 1 cut(s) 637
Fnu4HI GCNGC 2 cut(s) 225, 525
FokI GGATG 1 cut(s) 214
FriOI GRGCYC 2 cut(s) 67, 724
Fsp4HI GCNGC 2 cut(s) 225, 525
FspBI CTAG 3 cut(s) 117, 866, 902
GluI GCNGC 2 cut(s) 225, 525
HaeIII GGCC 1 cut(s) 692
HapII CCGG 2 cut(s) 188, 689
HgaI GACGC 1 cut(s) 132
Hin1I GRCGYC 1 cut(s) 124
Hin1II CATG 3 cut(s) 318, 620, 673
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 8 cut(s) 239, 248, 332, 442, 533, 705, 770, 974
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 2 cut(s) 188, 689
Hpy166II GTNNAC 2 cut(s) 391, 638
Hpy188I TCNGA 6 cut(s) 35, 376, 430, 544, 588, 852
Hpy188III TCNNGA 6 cut(s) 243, 329, 415, 600, 678, 931
Hpy8I GTNNAC 2 cut(s) 391, 638
Hpy99I CGWCG 3 cut(s) 126, 215, 862
HpyAV CCTTC 2 cut(s) 262, 995
HpyCH4III ACNGT 2 cut(s) 412, 846
HpyCH4IV ACGT 2 cut(s) 169, 860
HpyCH4V TGCA 3 cut(s) 227, 361, 731
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 143, 224, 455, 728, 809
HpyF3I CTNAG 6 cut(s) 306, 437, 579, 734, 849, 952
HpySE526I ACGT 2 cut(s) 169, 860
Hsp92I GRCGYC 1 cut(s) 124
Hsp92II CATG 3 cut(s) 318, 620, 673
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 2 cut(s) 206, 583
LmnI GCTCC 3 cut(s) 100, 142, 719
Lsp1109I GCAGC 2 cut(s) 211, 536
LweI GCATC 2 cut(s) 236, 348
MaeI CTAG 3 cut(s) 117, 866, 902
MaeII ACGT 2 cut(s) 169, 860
MalI GATC 2 cut(s) 208, 585
MboI GATC 2 cut(s) 206, 583
MboII GAAGA 7 cut(s) 286, 305, 476, 509, 542, 968, 980
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 4 cut(s) 67, 97, 139, 724
MluCI AATT 1 cut(s) 43
MlyI GAGTC 3 cut(s) 341, 714, 779
MmeI TCCRAC 1 cut(s) 601
MseI TTAA 6 cut(s) 23, 201, 339, 390, 663, 788
MslI CAYNNNNRTG 1 cut(s) 132
MspI CCGG 2 cut(s) 188, 689
MspR9I CCNGG 2 cut(s) 162, 740
Mva1269I GAATGC 1 cut(s) 684
MvaI CCWGG 2 cut(s) 162, 740
MwoI GCNNNNNNNGC 6 cut(s) 85, 143, 224, 455, 728, 809
NdeII GATC 2 cut(s) 206, 583
NlaIII CATG 3 cut(s) 318, 620, 673
NlaIV GGNNCC 2 cut(s) 721, 945
PaeR7I CTCGAG 1 cut(s) 141
PctI GAATGC 1 cut(s) 684
PfeI GAWTC 5 cut(s) 239, 248, 442, 533, 974
PfoI TCCNGGA 1 cut(s) 160
PkrI GCNGC 2 cut(s) 226, 526
PleI GAGTC 3 cut(s) 340, 713, 778
PpsI GAGTC 3 cut(s) 340, 713, 778
Psp124BI GAGCTC 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 160, 738
PspGI CCWGG 2 cut(s) 160, 738
PspN4I GGNNCC 2 cut(s) 721, 945
PspPI GGNCC 1 cut(s) 944
PspXI VCTCGAGB 1 cut(s) 141
PsuI RGATCY 1 cut(s) 583
RsaI GTAC 2 cut(s) 475, 917
RsaNI GTAC 2 cut(s) 474, 916
RseI CAYNNNNRTG 1 cut(s) 132
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 6 cut(s) 23, 201, 339, 390, 663, 788
SatI GCNGC 2 cut(s) 225, 525
Sau3AI GATC 2 cut(s) 206, 583
Sau96I GGNCC 1 cut(s) 944
SchI GAGTC 3 cut(s) 341, 714, 779
ScrFI CCNGG 2 cut(s) 162, 740
SduI GDGCHC 4 cut(s) 67, 97, 139, 724
SfaNI GCATC 2 cut(s) 236, 348
SfcI CTRYAG 2 cut(s) 58, 804
Sfr274I CTCGAG 1 cut(s) 141
SinI GGWCC 1 cut(s) 944
SlaI CTCGAG 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 132
SmlI CTYRAG 2 cut(s) 141, 329
SmoI CTYRAG 2 cut(s) 141, 329
Sse9I AATT 1 cut(s) 43
SsiI CCGC 4 cut(s) 77, 449, 835, 963
SspMI CTAG 3 cut(s) 117, 866, 902
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 2 cut(s) 160, 738
StyI CCWWGG 1 cut(s) 865
TaaI ACNGT 2 cut(s) 412, 846
TaiI ACGT 2 cut(s) 172, 863
TaqI TCGA 3 cut(s) 142, 205, 857
TasI AATT 1 cut(s) 43
TatI WGTACW 1 cut(s) 915
TfiI GAWTC 5 cut(s) 239, 248, 442, 533, 974
Tru1I TTAA 6 cut(s) 23, 201, 339, 390, 663, 788
Tru9I TTAA 6 cut(s) 23, 201, 339, 390, 663, 788
TscAI CASTG 3 cut(s) 139, 583, 738
TseI GCWGC 2 cut(s) 224, 524
TspDTI ATGAA 3 cut(s) 605, 658, 987
TspGWI ACGGA 2 cut(s) 199, 376
TspRI CASTG 3 cut(s) 139, 583, 738
VpaK11BI GGWCC 1 cut(s) 944
XhoI CTCGAG 1 cut(s) 141
XmaJI CCTAGG 1 cut(s) 865
XmiI GTMKAC 1 cut(s) 637
XspI CTAG 3 cut(s) 117, 866, 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.