MD04G1175000.v1.1

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009460
Physical Location & Seq
Forward (+)
26624308 .. 26627251
2944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1175000.v1.1.491

Sequence Viewer

Length: 945 bp
ATGAGTGTGACATTGCAGTCCGTCTCGCTCTTAAACCCTGACCCTAGCAGCTGTGGATTGAAGAAGTCACCTTCGTGTTCTTCTCTGCGGTTTGGACAATTGATTGCAGCACGTGGTTACGGAGCCTTGCCTATTGCCGTCCCGAAAGTGCATTCCGGCGCTTCTCTGTCCCTGCACCGGCGAAGCTTGTGGACTCGGCCGATTGTCGCCGTCTCTGCTTCCCAGGACGAATCTCATTCAGACATTGAGGTGGAGGATGAAAACAGCAATGCCAAACCGAAATCTGAAGAATCAGAAGAAGCTTGGAAACAAACTCTGGGTTCTTTCAAAGAACAAACCTTGAAGATGCGAAGCCTCTCGCGGGAATTCTCGAAGAAAGCATTGGTTATTATGAAAGATACTTCGGAGCAGTTAAAAATACATGCTGATAAGGCAAGGGATTTGAGTGGGATAGCAAATGAAATCAGTGAACATTTTATCACCATAGCTGAAGCCAATTCCCCTGAGCCAGTGAGGGAAATTGTTGAAGCATATCATATATCAACTATTGATCCCAATGATGCTGCGCAAGTCCGCGACTTTCGCGTTGGGATACCTTATGGTCTTTTGCTTTCTGTGGGTGGATTCCTTTCCTTCTTGGTAACGGGAAGCATTTCTGCAATTAGGTTTGGGGTTATCCTTGGTGGCACTCTTTTGTTTCTAAGCGTTTCAAGTTTAACATCATATAGAAAAGGAGAATCATCTCCCTTTTTCTTGACAGGGCAGACAGCTATATCCAGTATAATTTTTCTAAGGGAGGTAATCGTTCTGGCTCAGAGACCATCGTTTCCGAATATTTTAACAACCCTTGTCAGTGGAGCAGTTGTAGCTTTCTATTTCAATAAGATTGTACAGAATCGCAAGGATCAAAAGGGTCCCAACTTTGAGAAGGAGACAGAGAGTTAG

Protein Analysis

315

Amino Acids

34.26

Weight (kDa)

7.78

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 196 - 292 2.4e-13 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 16
Acc16I TGCGCA 1 cut(s) 567
AccII CGCG 3 cut(s) 361, 576, 585
AciI CCGC 3 cut(s) 88, 361, 574
AclWI GGATC 2 cut(s) 545, 912
AcoI YGGCCR 1 cut(s) 197
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 2 cut(s) 306, 510
AcvI CACGTG 1 cut(s) 113
AfaI GTAC 1 cut(s) 891
AfiI CCNNNNNNNGG 2 cut(s) 177, 361
AgsI TTSAA 6 cut(s) 61, 328, 343, 527, 711, 880
AjnI CCWGG 1 cut(s) 222
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AleI CACNNNNGTG 1 cut(s) 73
AluBI AGCT 6 cut(s) 51, 186, 302, 488, 770, 869
AluI AGCT 6 cut(s) 51, 186, 302, 488, 770, 869
Alw26I GTCTC 4 cut(s) 28, 217, 811, 926
AlwI GGATC 2 cut(s) 545, 912
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 3 cut(s) 48, 107, 563
ApoI RAATTY 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 652
AspLEI GCGC 2 cut(s) 161, 568
AspS9I GGNCC 1 cut(s) 914
AsuHPI GGTGA 2 cut(s) 60, 472
AvaII GGWCC 1 cut(s) 914
BbrPI CACGTG 1 cut(s) 113
BbvI GCAGC 3 cut(s) 60, 119, 550
BccI CCATC 1 cut(s) 829
BceAI ACGGC 2 cut(s) 122, 194
BciT130I CCWGG 1 cut(s) 224
BciVI GTATCC 1 cut(s) 585
BcoDI GTCTC 4 cut(s) 28, 217, 811, 926
BfaI CTAG 1 cut(s) 45
BfoI RGCGCY 1 cut(s) 162
BfuI GTATCC 1 cut(s) 585
BisI GCNGC 3 cut(s) 49, 108, 564
BlsI GCNGC 3 cut(s) 50, 109, 565
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 914
BmgT120I GGNCC 1 cut(s) 914
BmiI GGNNCC 3 cut(s) 124, 915, 916
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 2 cut(s) 336, 550
Bpu10I CCTNAGC 1 cut(s) 504
BsaAI YACGTR 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 811
BsaJI CCNNGG 2 cut(s) 222, 679
BsaXI ACNNNNNCTCC 2 cut(s) 849, 879
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 361
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 509, 777
Bse3DI GCAATG 2 cut(s) 11, 274
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 2 cut(s) 222, 679
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 2 cut(s) 177, 361
BseMI GCAATG 2 cut(s) 11, 274
BseMII CTCAG 2 cut(s) 495, 827
BseNI ACTGG 2 cut(s) 509, 777
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 3 cut(s) 60, 119, 550
BsgI GTGCAG 1 cut(s) 158
Bsh1236I CGCG 3 cut(s) 361, 576, 585
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 1 cut(s) 199
BsiEI CGRYCG 1 cut(s) 200
BsiSI CCGG 2 cut(s) 156, 178
BslFI GGGAC 3 cut(s) 125, 154, 900
BslI CCNNNNNNNGG 2 cut(s) 177, 361
BsmAI GTCTC 4 cut(s) 28, 217, 811, 926
BsmBI CGTCTC 2 cut(s) 28, 217
BsmFI GGGAC 3 cut(s) 125, 154, 900
BsmI GAATGC 1 cut(s) 151
BsnI GGCC 1 cut(s) 199
Bso31I GGTCTC 1 cut(s) 811
Bsp1407I TGTACA 1 cut(s) 889
Bsp143I GATC 2 cut(s) 550, 904
BspACI CCGC 3 cut(s) 88, 361, 574
BspANI GGCC 1 cut(s) 199
BspCNI CTCAG 2 cut(s) 496, 826
BspFNI CGCG 3 cut(s) 361, 576, 585
BspLI GGNNCC 3 cut(s) 124, 915, 916
BspPI GGATC 2 cut(s) 545, 912
BspTNI GGTCTC 1 cut(s) 811
BsrDI GCAATG 2 cut(s) 11, 274
BsrFI RCCGGY 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 889
BsrI ACTGG 2 cut(s) 509, 777
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 222, 679
BssMI GATC 2 cut(s) 550, 904
BssT1I CCWWGG 1 cut(s) 679
Bst2UI CCWGG 1 cut(s) 224
BstAUI TGTACA 1 cut(s) 889
BstBAI YACGTR 1 cut(s) 113
BstDEI CTNAG 4 cut(s) 504, 701, 791, 813
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 3 cut(s) 361, 576, 585
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 2 cut(s) 161, 568
BstKTI GATC 2 cut(s) 553, 907
BstMAI GTCTC 4 cut(s) 28, 217, 811, 926
BstMBI GATC 2 cut(s) 550, 904
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 4 cut(s) 215, 431, 582, 866
BstNI CCWGG 1 cut(s) 224
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 222
BstUI CGCG 3 cut(s) 361, 576, 585
BstV1I GCAGC 3 cut(s) 60, 119, 550
BstZI CGGCCG 1 cut(s) 197
BsuI GTATCC 1 cut(s) 585
BsuRI GGCC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 262
BtsIMutI CAGTG 3 cut(s) 472, 516, 859
CfoI GCGC 2 cut(s) 161, 568
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 914
Csp6I GTAC 1 cut(s) 890
CviAII CATG 1 cut(s) 422
CviQI GTAC 1 cut(s) 890
DdeI CTNAG 4 cut(s) 504, 701, 791, 813
DpnI GATC 2 cut(s) 552, 906
DpnII GATC 2 cut(s) 550, 904
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 197
EagI CGGCCG 1 cut(s) 197
EclXI CGGCCG 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 679
Eco31I GGTCTC 1 cut(s) 811
Eco47I GGWCC 1 cut(s) 914
Eco52I CGGCCG 1 cut(s) 197
Eco57I CTGAAG 2 cut(s) 306, 510
Eco72I CACGTG 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 222
EcoT14I CCWWGG 1 cut(s) 679
ErhI CCWWGG 1 cut(s) 679
Esp3I CGTCTC 2 cut(s) 28, 217
FaeI CATG 1 cut(s) 425
FaqI GGGAC 3 cut(s) 125, 154, 900
FatI CATG 1 cut(s) 421
FauI CCCGC 1 cut(s) 354
Fnu4HI GCNGC 3 cut(s) 49, 108, 564
FokI GGATG 1 cut(s) 269
Fsp4HI GCNGC 3 cut(s) 49, 108, 564
FspBI CTAG 1 cut(s) 45
FspI TGCGCA 1 cut(s) 567
GlaI GCGC 2 cut(s) 160, 567
GluI GCNGC 3 cut(s) 49, 108, 564
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 1 cut(s) 199
HapII CCGG 2 cut(s) 156, 178
HhaI GCGC 2 cut(s) 161, 568
Hin1II CATG 1 cut(s) 425
Hin6I GCGC 2 cut(s) 159, 566
HinP1I GCGC 2 cut(s) 159, 566
HindIII AAGCTT 2 cut(s) 184, 300
HinfI GANTC 6 cut(s) 193, 230, 290, 624, 737, 895
HpaII CCGG 2 cut(s) 156, 178
HphI GGTGA 2 cut(s) 60, 472
Hpy166II GTNNAC 2 cut(s) 192, 470
Hpy188I TCNGA 6 cut(s) 241, 286, 295, 406, 816, 831
Hpy188III TCNNGA 3 cut(s) 142, 370, 754
Hpy8I GTNNAC 2 cut(s) 192, 470
HpyAV CCTTC 3 cut(s) 81, 643, 922
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 5 cut(s) 16, 107, 151, 175, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 431, 582, 866
HpyF3I CTNAG 4 cut(s) 504, 701, 791, 813
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 1 cut(s) 425
HspAI GCGC 2 cut(s) 159, 566
KflI GGGWCCC 1 cut(s) 914
Kzo9I GATC 2 cut(s) 550, 904
LmnI GCTCC 3 cut(s) 122, 406, 857
Lsp1109I GCAGC 3 cut(s) 60, 119, 550
LweI GCATC 2 cut(s) 336, 550
MaeI CTAG 1 cut(s) 45
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 4 cut(s) 7, 66, 116, 640
MalI GATC 2 cut(s) 552, 906
MboI GATC 2 cut(s) 550, 904
MboII GAAGA 6 cut(s) 72, 73, 299, 308, 355, 385
MfeI CAATTG 1 cut(s) 98
MluCI AATT 6 cut(s) 98, 365, 496, 519, 660, 783
MlyI GAGTC 1 cut(s) 187
MnlI CCTC 5 cut(s) 241, 247, 365, 507, 790
MroXI GAANNNNTTC 1 cut(s) 652
MseI TTAA 4 cut(s) 32, 413, 716, 839
MslI CAYNNNNRTG 2 cut(s) 73, 248
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 2 cut(s) 156, 178
MspR9I CCNGG 1 cut(s) 224
MunI CAATTG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 151
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 3 cut(s) 361, 576, 585
MwoI GCNNNNNNNGC 4 cut(s) 215, 431, 582, 866
NdeII GATC 2 cut(s) 550, 904
NlaIII CATG 1 cut(s) 425
NlaIV GGNNCC 3 cut(s) 124, 915, 916
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 2 cut(s) 7, 66
NsbI TGCGCA 1 cut(s) 567
NspI RCATGY 1 cut(s) 425
OliI CACNNNNGTG 1 cut(s) 73
PctI GAATGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 652
PfeI GAWTC 5 cut(s) 230, 290, 624, 737, 895
PkrI GCNGC 3 cut(s) 50, 109, 565
PleI GAGTC 1 cut(s) 187
PmaCI CACGTG 1 cut(s) 113
PmlI CACGTG 1 cut(s) 113
PpsI GAGTC 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 914
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 1 cut(s) 222
PspCI CACGTG 1 cut(s) 113
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 3 cut(s) 124, 915, 916
PspPI GGNCC 1 cut(s) 914
PspPPI RGGWCCY 1 cut(s) 914
PvuII CAGCTG 1 cut(s) 51
RsaI GTAC 1 cut(s) 891
RsaNI GTAC 1 cut(s) 890
RseI CAYNNNNRTG 2 cut(s) 73, 248
SaqAI TTAA 4 cut(s) 32, 413, 716, 839
SatI GCNGC 3 cut(s) 49, 108, 564
Sau3AI GATC 2 cut(s) 550, 904
Sau96I GGNCC 1 cut(s) 914
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 224
SfaNI GCATC 2 cut(s) 336, 550
SgrAI CRCCGGYG 1 cut(s) 177
SinI GGWCC 1 cut(s) 914
SmiMI CAYNNNNRTG 2 cut(s) 73, 248
Sse9I AATT 6 cut(s) 98, 365, 496, 519, 660, 783
SsiI CCGC 3 cut(s) 88, 361, 574
SspI AATATT 1 cut(s) 835
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 1 cut(s) 222
StyI CCWWGG 1 cut(s) 679
TaiI ACGT 1 cut(s) 115
TaqI TCGA 1 cut(s) 371
TasI AATT 6 cut(s) 98, 365, 496, 519, 660, 783
TatI WGTACW 1 cut(s) 889
TfiI GAWTC 5 cut(s) 230, 290, 624, 737, 895
Tru1I TTAA 4 cut(s) 32, 413, 716, 839
Tru9I TTAA 4 cut(s) 32, 413, 716, 839
TscAI CASTG 3 cut(s) 472, 516, 859
TseFI GTSAC 2 cut(s) 7, 66
TseI GCWGC 3 cut(s) 48, 107, 563
Tsp45I GTSAC 2 cut(s) 7, 66
TspDTI ATGAA 3 cut(s) 273, 407, 474
TspGWI ACGGA 2 cut(s) 10, 135
TspRI CASTG 3 cut(s) 472, 516, 859
VpaK11BI GGWCC 1 cut(s) 914
XapI RAATTY 1 cut(s) 365
XceI RCATGY 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 652
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.