Rh3CG106400

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0009460
Physical Location & Seq
Reverse (-)
8379262 .. 8383150
3889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG106400.1

Sequence Viewer

Length: 1293 bp
ATGCTCCACGCTATGGTACTACAGAAGAAGCCGGCTCACCAACCGAGGACCCACCGAAAGCGAATGCACCCGGCCCGCACCAGATCGAAAGCACCACGGTGGCGCTTTCCAAAGATTCAGCGGGCTCATCGACTGCCTATTGATTACGTGTCACTCAGCCATCAAATCATTTTCTCTCACTTCCCACCTGAGACGCGTGTACCTCACGCCCCTCGCCGCACTTTAGTGAACTCCACCCCGCTTCTTCTGGGTTTCATGATTTCATCTCTCTCTCTCTCTCTCTCTCTCACTCTCTCTTTATATATAAAGAGCTCCGACGCCTCTTACCTAACCTCTTCGTCAGCTCCGTCTCAATTTTTAAGCCCCCTTTGTCAAACTCTCACAATGAGCGTGACGTTGCAGTCGGTCTCTCTCATAAACCCTAACCCTAGCTCTCGCTTCCAGAAGGCGGCGGCGACGACGACTCCGGTAGCCTTGTGTTCTCGGCGGTTCGATCATTTCGTCGGTGCTCGCGGTTATGGAGGAGCTCCGGTTGCCGTTCCGAAAGGGCTCGGCTGTGGTGGCTCGCTGTCTCTGAGCCGGCGGAGCTTGTGGTGCCGGCCAATTGTAGCCGTCGCTGCCTCGCAAGATGAATCGAAGCATCCAGAGGTTGAGGTGGAGAAGGAAAAGGAAAGTGCCAAGTTGAAAGCTGAAGAATCAGAAGAAGCTTGGGAACAGACTTTGGCTTCTTTCAAAGAACAAGCTTTAAAGATGCAAAGTGTCTCACAGGAAGCGTATGAGATATATTCTAAGAAAGCCTTGGTTATTTTGAAGGAAACGTCAGAGCAGTTGAAAATACAGGCTGATAAGGCAAGGTATGATATCACTGAGATAGCTAAAGAGATCAGCGTTGAAGGTAAAGAATATATCTCCACAGCCGCAAAGAATTCCCCTGAACCAGTGAAGGAAATTGTTGAAACATTCAATTCATCAACAGACGATCTGAATGACGTCACAAAAATCCGCGACTTTCATGTTGGGATACCATATGGTCTGGTTCTTTCTCTTGGCGGCTTCCTTTCCTTCATGGTAACAGGAAGCACTGCTGGAATTAGGTTTGGGGTTATCCTTGGTGGCATTCTTTTGGCTTTCAGTGTTTCAAGTTTGAGATCATATAAGCAAGGAGAATCATTTCCTTTGGCCTTGAAAGGGCAGGCAGCTATAGCCAGTATAATTTTTCTGAGGGAGACATGCCTACTGGCTCAGAGATCATGGATTCCATATTTGTTGAGAACCCTTGTCAGGTTTTTGTGA

Protein Analysis

430

Amino Acids

47.57

Weight (kDa)

9.7

Isoelectric Point (pI)

50.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 339 - 423 1.4e-11 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 400
AatII GACGTC 1 cut(s) 993
AccB1I GGYRCC 1 cut(s) 594
AccB7I CCANNNNNTGG 1 cut(s) 13
AccII CGCG 3 cut(s) 196, 513, 1005
AcoI YGGCCR 1 cut(s) 599
AcsI RAATTY 1 cut(s) 925
AcuI CTGAAG 1 cut(s) 711
AcyI GRCGYC 2 cut(s) 318, 990
AfaI GTAC 2 cut(s) 18, 201
AfiI CCNNNNNNNGG 4 cut(s) 13, 448, 1188, 1281
AflIII ACRYGT 2 cut(s) 147, 194
AgsI TTSAA 9 cut(s) 685, 733, 811, 832, 893, 956, 964, 1140, 1186
AleI CACNNNNGTG 2 cut(s) 97, 224
Alw21I GWGCWC 3 cut(s) 314, 511, 529
Alw26I GTCTC 6 cut(s) 185, 354, 412, 576, 766, 1220
AoxI GGCC 3 cut(s) 72, 599, 1179
ApeKI GCWGC 2 cut(s) 617, 1196
ApoI RAATTY 1 cut(s) 925
Asp700I GAANNNNTTC 1 cut(s) 1170
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 2 cut(s) 48, 73
AsuC2I CCSGG 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 29
AvaII GGWCC 1 cut(s) 48
BaeI ACNNNNGTAYC 2 cut(s) 183, 216
BanI GGYRCC 1 cut(s) 594
BanII GRGCYC 4 cut(s) 127, 314, 529, 552
Bbv12I GWGCWC 3 cut(s) 314, 511, 529
BbvI GCAGC 2 cut(s) 604, 1208
BccI CCATC 1 cut(s) 168
BceAI ACGGC 2 cut(s) 521, 596
BciVI GTATCC 1 cut(s) 1014
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 6 cut(s) 185, 354, 412, 576, 766, 1220
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 2 cut(s) 20, 1200
BfoI RGCGCY 1 cut(s) 106
BfuI GTATCC 1 cut(s) 1014
BisI GCNGC 7 cut(s) 217, 450, 453, 618, 918, 1051, 1197
BlsI GCNGC 7 cut(s) 218, 451, 454, 619, 919, 1052, 1198
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 2 cut(s) 48, 73
BmiI GGNNCC 2 cut(s) 50, 596
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 2 cut(s) 649, 741
BpuMI CCSGG 1 cut(s) 71
BsaAI YACGTR 1 cut(s) 148
BsaHI GRCGYC 2 cut(s) 318, 990
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 4 cut(s) 44, 95, 798, 1108
BsaWI WCCGGW 2 cut(s) 466, 529
BsaXI ACNNNNNCTCC 4 cut(s) 448, 478, 516, 546
Bsc4I CCNNNNNNNGG 4 cut(s) 13, 448, 1188, 1281
Bse118I RCCGGY 3 cut(s) 31, 579, 597
Bse1I ACTGG 3 cut(s) 938, 1206, 1242
BseDI CCNNGG 4 cut(s) 44, 95, 798, 1108
BseGI GGATG 1 cut(s) 640
BseLI CCNNNNNNNGG 4 cut(s) 13, 448, 1188, 1281
BseMII CTCAG 6 cut(s) 169, 180, 566, 858, 1211, 1256
BseNI ACTGG 3 cut(s) 938, 1206, 1242
BseRI GAGGAG 1 cut(s) 537
BseXI GCAGC 2 cut(s) 604, 1208
Bsh1236I CGCG 3 cut(s) 196, 513, 1005
BshFI GGCC 3 cut(s) 74, 601, 1181
BshNI GGYRCC 1 cut(s) 594
BsiHKAI GWGCWC 3 cut(s) 314, 511, 529
BsiSI CCGG 6 cut(s) 32, 71, 467, 530, 580, 598
BslI CCNNNNNNNGG 4 cut(s) 13, 448, 1188, 1281
BsmAI GTCTC 6 cut(s) 185, 354, 412, 576, 766, 1220
BsmBI CGTCTC 2 cut(s) 185, 354
BsmI GAATGC 2 cut(s) 69, 1116
BsnI GGCC 3 cut(s) 74, 601, 1181
Bso31I GGTCTC 1 cut(s) 412
Bsp1286I GDGCHC 5 cut(s) 127, 314, 511, 529, 552
Bsp143I GATC 6 cut(s) 83, 493, 882, 979, 1148, 1247
BspANI GGCC 3 cut(s) 74, 601, 1181
BspCNI CTCAG 6 cut(s) 168, 181, 567, 859, 1212, 1255
BspFNI CGCG 3 cut(s) 196, 513, 1005
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 50, 596
BspT107I GGYRCC 1 cut(s) 594
BspTNI GGTCTC 1 cut(s) 412
BsrFI RCCGGY 3 cut(s) 31, 579, 597
BsrI ACTGG 3 cut(s) 938, 1206, 1242
BssAI RCCGGY 3 cut(s) 31, 579, 597
BssECI CCNNGG 4 cut(s) 44, 95, 798, 1108
BssMI GATC 6 cut(s) 83, 493, 882, 979, 1148, 1247
BssNI GRCGYC 2 cut(s) 318, 990
BssT1I CCWWGG 2 cut(s) 798, 1108
Bst4CI ACNGT 1 cut(s) 99
Bst6I CTCTTC 1 cut(s) 340
BstACI GRCGYC 2 cut(s) 318, 990
BstBAI YACGTR 1 cut(s) 148
BstC8I GCNNGC 8 cut(s) 33, 76, 123, 511, 566, 581, 599, 1194
BstDEI CTNAG 7 cut(s) 155, 189, 575, 789, 867, 1220, 1242
BstDSI CCRYGG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 640
BstFNI CGCG 3 cut(s) 196, 513, 1005
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 6 cut(s) 86, 496, 885, 982, 1151, 1250
BstMAI GTCTC 6 cut(s) 185, 354, 412, 576, 766, 1220
BstMBI GATC 6 cut(s) 83, 493, 882, 979, 1148, 1247
BstMWI GCNNNNNNNGC 7 cut(s) 533, 561, 585, 594, 617, 848, 1202
BstNSI RCATGY 1 cut(s) 1233
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 2 cut(s) 20, 1200
BstUI CGCG 3 cut(s) 196, 513, 1005
BstV1I GCAGC 2 cut(s) 604, 1208
BsuI GTATCC 1 cut(s) 1014
BsuRI GGCC 3 cut(s) 74, 601, 1181
BtgI CCRYGG 1 cut(s) 95
BtsCI GGATG 1 cut(s) 640
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 4 cut(s) 864, 945, 1080, 1138
Cac8I GCNNGC 8 cut(s) 33, 76, 123, 511, 566, 581, 599, 1194
CciI TCATGA 1 cut(s) 255
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 3 cut(s) 31, 579, 597
Cfr13I GGNCC 2 cut(s) 48, 73
CseI GACGC 2 cut(s) 202, 326
Csp6I GTAC 2 cut(s) 17, 200
CviAII CATG 5 cut(s) 256, 1013, 1066, 1230, 1251
CviQI GTAC 2 cut(s) 17, 200
DdeI CTNAG 7 cut(s) 155, 189, 575, 789, 867, 1220, 1242
DpnI GATC 6 cut(s) 85, 495, 884, 981, 1150, 1249
DpnII GATC 6 cut(s) 83, 493, 882, 979, 1148, 1247
DraI TTTAAA 1 cut(s) 747
DrdI GACNNNNNNGTC 1 cut(s) 400
DseDI GACNNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 599
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
EciI GGCGGA 1 cut(s) 598
Ecl136II GAGCTC 2 cut(s) 312, 527
Eco130I CCWWGG 2 cut(s) 798, 1108
Eco24I GRGCYC 4 cut(s) 127, 314, 529, 552
Eco31I GGTCTC 1 cut(s) 412
Eco32I GATATC 1 cut(s) 862
Eco47I GGWCC 1 cut(s) 48
Eco53kI GAGCTC 2 cut(s) 312, 527
Eco57I CTGAAG 1 cut(s) 711
EcoICRI GAGCTC 2 cut(s) 312, 527
EcoO109I RGGNCCY 1 cut(s) 48
EcoRI GAATTC 1 cut(s) 925
EcoRV GATATC 1 cut(s) 862
EcoT14I CCWWGG 2 cut(s) 798, 1108
EcoT38I GRGCYC 4 cut(s) 127, 314, 529, 552
ErhI CCWWGG 2 cut(s) 798, 1108
Esp3I CGTCTC 2 cut(s) 185, 354
FaeI CATG 5 cut(s) 259, 1016, 1069, 1233, 1254
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FatI CATG 5 cut(s) 255, 1012, 1065, 1229, 1250
FauI CCCGC 3 cut(s) 83, 114, 246
FauNDI CATATG 1 cut(s) 1027
Fnu4HI GCNGC 7 cut(s) 217, 450, 453, 618, 918, 1051, 1197
FokI GGATG 1 cut(s) 627
FriOI GRGCYC 4 cut(s) 127, 314, 529, 552
Fsp4HI GCNGC 7 cut(s) 217, 450, 453, 618, 918, 1051, 1197
FspBI CTAG 1 cut(s) 429
GlaI GCGC 1 cut(s) 104
GluI GCNGC 7 cut(s) 217, 450, 453, 618, 918, 1051, 1197
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 3 cut(s) 74, 601, 1181
HapII CCGG 6 cut(s) 32, 71, 467, 530, 580, 598
HgaI GACGC 2 cut(s) 202, 326
HhaI GCGC 1 cut(s) 105
Hin1I GRCGYC 2 cut(s) 318, 990
Hin1II CATG 5 cut(s) 259, 1016, 1069, 1233, 1254
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HindIII AAGCTT 2 cut(s) 705, 741
HinfI GANTC 6 cut(s) 115, 463, 632, 695, 1166, 1255
HpaII CCGG 6 cut(s) 32, 71, 467, 530, 580, 598
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 2 cut(s) 200, 229
Hpy188I TCNGA 8 cut(s) 316, 543, 576, 700, 823, 984, 1221, 1245
Hpy188III TCNNGA 3 cut(s) 256, 442, 644
Hpy8I GTNNAC 2 cut(s) 200, 229
Hpy99I CGWCG 5 cut(s) 320, 460, 463, 506, 617
HpyAV CCTTC 6 cut(s) 439, 655, 805, 887, 937, 1072
HpyCH4III ACNGT 1 cut(s) 99
HpyCH4IV ACGT 4 cut(s) 147, 395, 818, 990
HpyCH4V TGCA 3 cut(s) 67, 400, 754
HpyF10VI GCNNNNNNNGC 7 cut(s) 533, 561, 585, 594, 617, 848, 1202
HpyF3I CTNAG 7 cut(s) 155, 189, 575, 789, 867, 1220, 1242
HpySE526I ACGT 4 cut(s) 147, 395, 818, 990
Hsp92I GRCGYC 2 cut(s) 318, 990
Hsp92II CATG 5 cut(s) 259, 1016, 1069, 1233, 1254
HspAI GCGC 1 cut(s) 103
KroI GCCGGC 3 cut(s) 31, 579, 597
KroNI GCCGGC 3 cut(s) 33, 581, 599
Kzo9I GATC 6 cut(s) 83, 493, 882, 979, 1148, 1247
LmnI GCTCC 6 cut(s) 9, 317, 349, 524, 532, 585
Lsp1109I GCAGC 2 cut(s) 604, 1208
LweI GCATC 2 cut(s) 649, 741
MaeI CTAG 1 cut(s) 429
MaeII ACGT 4 cut(s) 147, 395, 818, 990
MaeIII GTNAC 4 cut(s) 150, 391, 991, 1069
MalI GATC 6 cut(s) 85, 495, 884, 981, 1150, 1249
MboI GATC 6 cut(s) 83, 493, 882, 979, 1148, 1247
MboII GAAGA 5 cut(s) 37, 236, 327, 704, 713
MfeI CAATTG 1 cut(s) 603
MhlI GDGCHC 5 cut(s) 127, 314, 511, 529, 552
MluCI AATT 7 cut(s) 353, 603, 925, 948, 964, 1089, 1212
MluI ACGCGT 1 cut(s) 194
MlyI GAGTC 1 cut(s) 457
MmeI TCCRAC 1 cut(s) 339
MroNI GCCGGC 3 cut(s) 31, 579, 597
MroXI GAANNNNTTC 1 cut(s) 1170
MseI TTAA 2 cut(s) 359, 746
MslI CAYNNNNRTG 2 cut(s) 97, 224
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 6 cut(s) 32, 71, 467, 530, 580, 598
MspR9I CCNGG 1 cut(s) 71
MunI CAATTG 1 cut(s) 603
Mva1269I GAATGC 2 cut(s) 69, 1116
MvnI CGCG 3 cut(s) 196, 513, 1005
MwoI GCNNNNNNNGC 7 cut(s) 533, 561, 585, 594, 617, 848, 1202
NaeI GCCGGC 3 cut(s) 33, 581, 599
NciI CCSGG 1 cut(s) 71
NdeI CATATG 1 cut(s) 1027
NdeII GATC 6 cut(s) 83, 493, 882, 979, 1148, 1247
NgoMIV GCCGGC 3 cut(s) 31, 579, 597
NlaIII CATG 5 cut(s) 259, 1016, 1069, 1233, 1254
NlaIV GGNNCC 2 cut(s) 50, 596
NmeAIII GCCGAG 2 cut(s) 463, 531
NmuCI GTSAC 3 cut(s) 150, 391, 991
NspI RCATGY 1 cut(s) 1233
OliI CACNNNNGTG 2 cut(s) 97, 224
PagI TCATGA 1 cut(s) 255
PcsI WCGNNNNNNNCGW 2 cut(s) 344, 498
PctI GAATGC 2 cut(s) 69, 1116
PdiI GCCGGC 3 cut(s) 33, 581, 599
PdmI GAANNNNTTC 1 cut(s) 1170
PfeI GAWTC 5 cut(s) 115, 632, 695, 1166, 1255
PflMI CCANNNNNTGG 1 cut(s) 13
PkrI GCNGC 7 cut(s) 218, 451, 454, 619, 919, 1052, 1198
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
Ppu21I YACGTR 1 cut(s) 148
PpuMI RGGWCCY 1 cut(s) 48
Psp124BI GAGCTC 2 cut(s) 314, 529
Psp5II RGGWCCY 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 50, 596
PspPI GGNCC 2 cut(s) 48, 73
PspPPI RGGWCCY 1 cut(s) 48
RsaI GTAC 2 cut(s) 18, 201
RsaNI GTAC 2 cut(s) 17, 200
RseI CAYNNNNRTG 2 cut(s) 97, 224
SacI GAGCTC 2 cut(s) 314, 529
SaqAI TTAA 2 cut(s) 359, 746
SatI GCNGC 7 cut(s) 217, 450, 453, 618, 918, 1051, 1197
Sau3AI GATC 6 cut(s) 83, 493, 882, 979, 1148, 1247
Sau96I GGNCC 2 cut(s) 48, 73
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 5 cut(s) 127, 314, 511, 529, 552
SfaNI GCATC 2 cut(s) 649, 741
SfcI CTRYAG 2 cut(s) 20, 1200
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 2 cut(s) 97, 224
Sse9I AATT 7 cut(s) 353, 603, 925, 948, 964, 1089, 1212
SspMI CTAG 1 cut(s) 429
SstI GAGCTC 2 cut(s) 314, 529
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 2 cut(s) 798, 1108
TaaI ACNGT 1 cut(s) 99
TaiI ACGT 4 cut(s) 150, 398, 821, 993
TaqI TCGA 4 cut(s) 86, 130, 492, 635
TaqII GACCGA 1 cut(s) 394
TasI AATT 7 cut(s) 353, 603, 925, 948, 964, 1089, 1212
TauI GCSGC 5 cut(s) 219, 452, 455, 920, 1053
TfiI GAWTC 5 cut(s) 115, 632, 695, 1166, 1255
Tru1I TTAA 2 cut(s) 359, 746
Tru9I TTAA 2 cut(s) 359, 746
TscAI CASTG 4 cut(s) 871, 945, 1087, 1138
TseFI GTSAC 3 cut(s) 150, 391, 991
TseI GCWGC 2 cut(s) 617, 1196
Tsp45I GTSAC 3 cut(s) 150, 391, 991
TspDTI ATGAA 6 cut(s) 244, 252, 645, 957, 1001, 1054
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 4 cut(s) 871, 945, 1087, 1138
Van91I CCANNNNNTGG 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 48
XapI RAATTY 1 cut(s) 925
XceI RCATGY 1 cut(s) 1233
XmnI GAANNNNTTC 1 cut(s) 1170
XspI CTAG 1 cut(s) 429
ZraI GACGTC 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.