pycom04g15440

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009460
Physical Location & Seq
Forward (+)
18326771 .. 18329045
2275 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15440.1

Sequence Viewer

Length: 945 bp
ATGAGTGTGACATTGCATTCCATCTCGCTCTTAAACCCTGACCCTAGCAGCTGTGGATTGAAGAAGTCACCTTCGTGTTCTTCTCTGCGGTTTGGACAATTGATTGCAGCACGTGGTTACCGAGGCTCGTATATTGCCGTCCCGAAAGTGCTTTCCGGCGCTTCTCTGTCCCTGCACCGGCGGAGCTTGTGGACTCGGCCAATTGTCGCCGTCTCTGCTTCCCAGGACGAATCTCATTCAGATATTGAGGTGGAGAATGAAAACAACAATGCCAAACCGAAATCTGAAGAATCAGAAGAACCTTCGAAACAAACTCTGGGTTCTTTCAAGGAACAAACCTTGAAGCTGCGAAGCCTCTCGCGGGAATTCTCAAAGAAAGCATTGGTTATTATGAAAGATACTTCGGAGCAGTTAAAAATATATGCTGATAAGGCAAGGGATGATTTGAGTGAGAGAGCAAATGAAATCAGTGAACATTATATCACCATAGCTGAAGCCAATTCCCCTGAGCCAGTGAGGGAAATTGTTGAAGCATATCATGTATCAGCTATTGATCCCAATGATGCTGTGCAAGTCCGTGACTTTCGCGTTGGGATACCTTATGGTCTTTGGCTTTCTGTGGGTGGATTCCTTTCCTTCTTGGTAACGGGAAGCATTTCTGCAATTAGGTTTGGGGTTATCCTTGGTGGCACTCTTTTGTTTCTAAGCGTTTCAAGTTTAAGATCATATATAAAAGGAAAATCATCTCCCTTTTTCTTGAAAGGGCAGACAGCTATATCCAGTATAATTTTTCTAAGGGAGGTAATCGTTCTGGCTGAGAGACCATCGTTTCCGAATATTTTAACAACCCTTGTCAGTGGAGCAGTTGTAGCTTTCTATTTCAGTAAGATTGTACAGAATCGCGAGGATCAAAAGGGTCCCAATTTTGAGGAGACAGAGAGTTAG

Protein Analysis

315

Amino Acids

34.53

Weight (kDa)

7.75

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 197 - 293 1.3e-12 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 361, 588, 903
AciI CCGC 3 cut(s) 88, 181, 361
AclWI GGATC 2 cut(s) 548, 915
AcoI YGGCCR 1 cut(s) 197
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 2 cut(s) 306, 513
AcvI CACGTG 1 cut(s) 113
AfaI GTAC 1 cut(s) 894
AfiI CCNNNNNNNGG 2 cut(s) 177, 361
AgsI TTSAA 6 cut(s) 61, 328, 343, 530, 714, 760
AjnI CCWGG 1 cut(s) 222
AleI CACNNNNGTG 1 cut(s) 73
AluBI AGCT 7 cut(s) 51, 186, 346, 491, 548, 773, 872
AluI AGCT 7 cut(s) 51, 186, 346, 491, 548, 773, 872
Alw26I GTCTC 3 cut(s) 217, 814, 926
AlwI GGATC 2 cut(s) 548, 915
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 3 cut(s) 48, 107, 346
ApoI RAATTY 1 cut(s) 365
Asp700I GAANNNNTTC 1 cut(s) 655
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 917
AsuHPI GGTGA 2 cut(s) 60, 475
AsuII TTCGAA 1 cut(s) 305
AvaII GGWCC 1 cut(s) 917
BbrPI CACGTG 1 cut(s) 113
BbvI GCAGC 3 cut(s) 60, 119, 333
BccI CCATC 2 cut(s) 29, 832
BceAI ACGGC 2 cut(s) 122, 194
BciT130I CCWGG 1 cut(s) 224
BciVI GTATCC 1 cut(s) 588
BcoDI GTCTC 3 cut(s) 217, 814, 926
BfaI CTAG 1 cut(s) 45
BfoI RGCGCY 1 cut(s) 162
BfuI GTATCC 1 cut(s) 588
BisI GCNGC 3 cut(s) 49, 108, 347
BlsI GCNGC 3 cut(s) 50, 109, 348
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 917
BmgT120I GGNCC 1 cut(s) 917
BmiI GGNNCC 2 cut(s) 918, 919
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 1 cut(s) 553
Bpu10I CCTNAGC 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 305
BsaAI YACGTR 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 814
BsaJI CCNNGG 3 cut(s) 121, 222, 682
BsaXI ACNNNNNCTCC 2 cut(s) 852, 882
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 361
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 512, 780
Bse3DI GCAATG 1 cut(s) 11
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 3 cut(s) 121, 222, 682
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 2 cut(s) 177, 361
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 2 cut(s) 498, 807
BseNI ACTGG 2 cut(s) 512, 780
BseRI GAGGAG 1 cut(s) 944
BseXI GCAGC 3 cut(s) 60, 119, 333
BsgI GTGCAG 1 cut(s) 158
Bsh1236I CGCG 3 cut(s) 361, 588, 903
BshFI GGCC 1 cut(s) 199
BsiSI CCGG 2 cut(s) 156, 178
BslFI GGGAC 3 cut(s) 125, 154, 903
BslI CCNNNNNNNGG 2 cut(s) 177, 361
BsmAI GTCTC 3 cut(s) 217, 814, 926
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 3 cut(s) 125, 154, 903
BsmI GAATGC 1 cut(s) 16
BsnI GGCC 1 cut(s) 199
Bso31I GGTCTC 1 cut(s) 814
Bsp119I TTCGAA 1 cut(s) 305
Bsp1407I TGTACA 1 cut(s) 892
Bsp143I GATC 3 cut(s) 553, 722, 907
Bsp68I TCGCGA 1 cut(s) 903
BspACI CCGC 3 cut(s) 88, 181, 361
BspANI GGCC 1 cut(s) 199
BspCNI CTCAG 2 cut(s) 499, 808
BspFNI CGCG 3 cut(s) 361, 588, 903
BspLI GGNNCC 2 cut(s) 918, 919
BspPI GGATC 2 cut(s) 548, 915
BspT104I TTCGAA 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 814
BsrDI GCAATG 1 cut(s) 11
BsrFI RCCGGY 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 892
BsrI ACTGG 2 cut(s) 512, 780
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 3 cut(s) 121, 222, 682
BssMI GATC 3 cut(s) 553, 722, 907
BssT1I CCWWGG 1 cut(s) 682
Bst2UI CCWGG 1 cut(s) 224
BstAUI TGTACA 1 cut(s) 892
BstBAI YACGTR 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 305
BstDEI CTNAG 4 cut(s) 507, 704, 794, 816
BstEII GGTNACC 1 cut(s) 116
BstF5I GGATG 1 cut(s) 445
BstFNI CGCG 3 cut(s) 361, 588, 903
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 556, 725, 910
BstMAI GTCTC 3 cut(s) 217, 814, 926
BstMBI GATC 3 cut(s) 553, 722, 907
BstMWI GCNNNNNNNGC 3 cut(s) 215, 431, 869
BstNI CCWGG 1 cut(s) 224
BstPI GGTNACC 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 222
BstUI CGCG 3 cut(s) 361, 588, 903
BstV1I GCAGC 3 cut(s) 60, 119, 333
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 445
BtsIMutI CAGTG 3 cut(s) 475, 519, 862
BtuMI TCGCGA 1 cut(s) 903
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 917
Csp6I GTAC 1 cut(s) 893
CviAII CATG 1 cut(s) 539
CviQI GTAC 1 cut(s) 893
DdeI CTNAG 4 cut(s) 507, 704, 794, 816
DpnI GATC 3 cut(s) 555, 724, 909
DpnII GATC 3 cut(s) 553, 722, 907
EaeI YGGCCR 1 cut(s) 197
EciI GGCGGA 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 682
Eco31I GGTCTC 1 cut(s) 814
Eco47I GGWCC 1 cut(s) 917
Eco57I CTGAAG 2 cut(s) 306, 513
Eco72I CACGTG 1 cut(s) 113
Eco91I GGTNACC 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 917
EcoO65I GGTNACC 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 222
EcoT14I CCWWGG 1 cut(s) 682
ErhI CCWWGG 1 cut(s) 682
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 1 cut(s) 542
FaqI GGGAC 3 cut(s) 125, 154, 903
FatI CATG 1 cut(s) 538
FauI CCCGC 1 cut(s) 354
Fnu4HI GCNGC 3 cut(s) 49, 108, 347
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 3 cut(s) 49, 108, 347
FspBI CTAG 1 cut(s) 45
GlaI GCGC 1 cut(s) 160
GluI GCNGC 3 cut(s) 49, 108, 347
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 1 cut(s) 199
HapII CCGG 2 cut(s) 156, 178
HhaI GCGC 1 cut(s) 161
Hin1II CATG 1 cut(s) 542
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 5 cut(s) 193, 230, 290, 627, 898
HpaII CCGG 2 cut(s) 156, 178
HphI GGTGA 2 cut(s) 60, 475
Hpy166II GTNNAC 2 cut(s) 192, 473
Hpy188I TCNGA 5 cut(s) 241, 286, 295, 406, 834
Hpy188III TCNNGA 3 cut(s) 142, 757, 902
Hpy8I GTNNAC 2 cut(s) 192, 473
HpyAV CCTTC 3 cut(s) 81, 312, 646
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 5 cut(s) 16, 107, 175, 571, 662
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 431, 869
HpyF3I CTNAG 4 cut(s) 507, 704, 794, 816
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 1 cut(s) 542
HspAI GCGC 1 cut(s) 159
KflI GGGWCCC 1 cut(s) 917
Kzo9I GATC 3 cut(s) 553, 722, 907
LmnI GCTCC 3 cut(s) 183, 406, 860
Lsp1109I GCAGC 3 cut(s) 60, 119, 333
LweI GCATC 1 cut(s) 553
MaeI CTAG 1 cut(s) 45
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 5 cut(s) 7, 66, 116, 578, 643
MalI GATC 3 cut(s) 555, 724, 909
MboI GATC 3 cut(s) 553, 722, 907
MboII GAAGA 4 cut(s) 72, 73, 299, 308
MfeI CAATTG 2 cut(s) 98, 201
MluCI AATT 8 cut(s) 98, 201, 365, 499, 522, 663, 786, 922
MlyI GAGTC 1 cut(s) 187
MnlI CCTC 7 cut(s) 116, 241, 365, 510, 793, 898, 922
MroXI GAANNNNTTC 1 cut(s) 655
MseI TTAA 4 cut(s) 32, 413, 719, 842
MslI CAYNNNNRTG 1 cut(s) 73
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 2 cut(s) 156, 178
MspR9I CCNGG 1 cut(s) 224
MunI CAATTG 2 cut(s) 98, 201
Mva1269I GAATGC 1 cut(s) 16
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 3 cut(s) 361, 588, 903
MwoI GCNNNNNNNGC 3 cut(s) 215, 431, 869
NdeII GATC 3 cut(s) 553, 722, 907
NlaIII CATG 1 cut(s) 542
NlaIV GGNNCC 2 cut(s) 918, 919
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 3 cut(s) 7, 66, 578
NruI TCGCGA 1 cut(s) 903
NspV TTCGAA 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 118
PctI GAATGC 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 655
PfeI GAWTC 4 cut(s) 230, 290, 627, 898
PkrI GCNGC 3 cut(s) 50, 109, 348
PleI GAGTC 1 cut(s) 187
PmaCI CACGTG 1 cut(s) 113
PmlI CACGTG 1 cut(s) 113
PpsI GAGTC 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 917
Psp5II RGGWCCY 1 cut(s) 917
Psp6I CCWGG 1 cut(s) 222
PspCI CACGTG 1 cut(s) 113
PspEI GGTNACC 1 cut(s) 116
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 2 cut(s) 918, 919
PspPI GGNCC 1 cut(s) 917
PspPPI RGGWCCY 1 cut(s) 917
PvuII CAGCTG 1 cut(s) 51
RruI TCGCGA 1 cut(s) 903
RsaI GTAC 1 cut(s) 894
RsaNI GTAC 1 cut(s) 893
RseI CAYNNNNRTG 1 cut(s) 73
SaqAI TTAA 4 cut(s) 32, 413, 719, 842
SatI GCNGC 3 cut(s) 49, 108, 347
Sau3AI GATC 3 cut(s) 553, 722, 907
Sau96I GGNCC 1 cut(s) 917
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 224
SfaNI GCATC 1 cut(s) 553
SfuI TTCGAA 1 cut(s) 305
SgrAI CRCCGGYG 1 cut(s) 177
SinI GGWCC 1 cut(s) 917
SmiMI CAYNNNNRTG 1 cut(s) 73
Sse9I AATT 8 cut(s) 98, 201, 365, 499, 522, 663, 786, 922
SsiI CCGC 3 cut(s) 88, 181, 361
SspI AATATT 1 cut(s) 838
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 1 cut(s) 222
StyI CCWWGG 1 cut(s) 682
TaiI ACGT 1 cut(s) 115
TaqI TCGA 1 cut(s) 305
TasI AATT 8 cut(s) 98, 201, 365, 499, 522, 663, 786, 922
TatI WGTACW 1 cut(s) 892
TfiI GAWTC 4 cut(s) 230, 290, 627, 898
Tru1I TTAA 4 cut(s) 32, 413, 719, 842
Tru9I TTAA 4 cut(s) 32, 413, 719, 842
TscAI CASTG 3 cut(s) 475, 519, 862
TseFI GTSAC 3 cut(s) 7, 66, 578
TseI GCWGC 3 cut(s) 48, 107, 346
Tsp45I GTSAC 3 cut(s) 7, 66, 578
TspDTI ATGAA 3 cut(s) 273, 407, 477
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 3 cut(s) 475, 519, 862
VpaK11BI GGWCC 1 cut(s) 917
XapI RAATTY 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 655
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.