Rw3G008560

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009460
Physical Location & Seq
Reverse (-)
7941117 .. 7943284
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G008560.1

Sequence Viewer

Length: 888 bp
ATGAGCGTGACGTTGCAGTCGGTCTCTCTCTTAAACCCTAACCCTAGCTCTCGCTTCCAGAAGGCGGCGGCGGCGACGACGACTCCGGTAGCCTTGTGTTCTCGGCGGTTCGATCATTTCGTCGGTGCTCGCGGGCTCGGCTGTGGTGGCTCGCTGTCTCTGAGCCGGCGGAGCTTGTGGTGCCGGCCAATTGTAGCCGTCGCTGCCTCGCAAGATGAATCGAAGCATCCAGAGGTTGAGGTGGAGAAGGAAAAGGAAAGTGCCAAGTTGAAAGCTGAAGAATCAGAAGAAGCTTGGGAACAGACTTTGGCTTCTTTCAAAGAACAAGCTTTAAAGATGCAAAGTGTCTCACAGGAAGCGTATGAGATATATTCTAAGAAAGCCTTGGTTGCTGATAAGGCAAGGTATGATATCACTGAGATAGCTAAAGAGATCAGCGTTGAAGGTAAAGAATATATCTCCACAGCCGCAAAGAATTCCCCTGAGCCAGTGAAGGAAATTGTTGAAACATTCAATTCATCAACAGACGATCTGAATGACGTCACAAAAATCCGCGACTTTCATGTCTGGTTTCTTTCTCTTGGTGGCTTCCTTTCCTTCATGGTAACAGGAAGCACCGCTGGAATTAGGTTTGGGGTTATCCTTGGTGGCATTCTTTTGGCTTTCAGTGTTTCAAGTTTGAGATCATATAAGCAAGGAGAATCATTTCCTTTGGCCTTGAAAGGGCAGGCAGCTATAGCCAGTATAATTTTTCTGAGGGAGACATGCCTACTGGCTCAGAGATCATGGATTCCATATTTGTTGAGAACCCTTGGAGGTGTTGTAGCTTTCTATGTCTATAGGATTGTACAGAATGGTAAACACCAAAAAGATTCGAGCTTTGAATGA

Protein Analysis

295

Amino Acids

32.48

Weight (kDa)

8.24

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_14 PF03647 191 - 278 1.5e-09 Transmembrane proteins 14C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 16, 563
AatII GACGTC 1 cut(s) 543
AccB1I GGYRCC 1 cut(s) 180
AccII CGCG 2 cut(s) 132, 555
AciI CCGC 9 cut(s) 65, 68, 71, 106, 132, 169, 468, 553, 618
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 540
AfaI GTAC 1 cut(s) 849
AfiI CCNNNNNNNGG 2 cut(s) 64, 723
AgsI TTSAA 8 cut(s) 271, 319, 443, 506, 514, 675, 721, 884
AluBI AGCT 9 cut(s) 48, 174, 275, 293, 329, 425, 734, 827, 879
AluI AGCT 9 cut(s) 48, 174, 275, 293, 329, 425, 734, 827, 879
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 4 cut(s) 28, 162, 352, 755
AoxI GGCC 2 cut(s) 185, 714
ApeKI GCWGC 2 cut(s) 203, 731
ApoI RAATTY 1 cut(s) 475
Asp700I GAANNNNTTC 1 cut(s) 705
BanI GGYRCC 1 cut(s) 180
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 190, 743
BceAI ACGGC 1 cut(s) 182
BcoDI GTCTC 4 cut(s) 28, 162, 352, 755
BfaI CTAG 1 cut(s) 45
BfmI CTRYAG 2 cut(s) 735, 838
BisI GCNGC 6 cut(s) 66, 69, 72, 204, 468, 732
BlsI GCNGC 6 cut(s) 67, 70, 73, 205, 469, 733
BmiI GGNNCC 1 cut(s) 182
BmsI GCATC 2 cut(s) 235, 327
Bpu10I CCTNAGC 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 540
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 3 cut(s) 384, 643, 811
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 807, 837
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 723
Bse118I RCCGGY 2 cut(s) 165, 183
Bse1I ACTGG 3 cut(s) 488, 741, 777
BseDI CCNNGG 3 cut(s) 384, 643, 811
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 2 cut(s) 64, 723
BseMII CTCAG 5 cut(s) 152, 408, 474, 746, 791
BseNI ACTGG 3 cut(s) 488, 741, 777
BseXI GCAGC 2 cut(s) 190, 743
Bsh1236I CGCG 2 cut(s) 132, 555
BshFI GGCC 2 cut(s) 187, 716
BshNI GGYRCC 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 3 cut(s) 86, 166, 184
BslI CCNNNNNNNGG 2 cut(s) 64, 723
BsmAI GTCTC 4 cut(s) 28, 162, 352, 755
BsmI GAATGC 1 cut(s) 651
BsnI GGCC 2 cut(s) 187, 716
Bso31I GGTCTC 1 cut(s) 28
Bsp1286I GDGCHC 2 cut(s) 130, 138
Bsp1407I TGTACA 1 cut(s) 847
Bsp143I GATC 5 cut(s) 112, 432, 529, 683, 782
BspACI CCGC 9 cut(s) 65, 68, 71, 106, 132, 169, 468, 553, 618
BspANI GGCC 2 cut(s) 187, 716
BspCNI CTCAG 5 cut(s) 153, 409, 475, 747, 790
BspFNI CGCG 2 cut(s) 132, 555
BspLI GGNNCC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 28
BsrFI RCCGGY 2 cut(s) 165, 183
BsrGI TGTACA 1 cut(s) 847
BsrI ACTGG 3 cut(s) 488, 741, 777
BssAI RCCGGY 2 cut(s) 165, 183
BssECI CCNNGG 3 cut(s) 384, 643, 811
BssMI GATC 5 cut(s) 112, 432, 529, 683, 782
BssNI GRCGYC 1 cut(s) 540
BssT1I CCWWGG 3 cut(s) 384, 643, 811
BstACI GRCGYC 1 cut(s) 540
BstAUI TGTACA 1 cut(s) 847
BstC8I GCNNGC 6 cut(s) 130, 134, 152, 167, 185, 729
BstDEI CTNAG 6 cut(s) 161, 375, 417, 483, 755, 777
BstF5I GGATG 1 cut(s) 226
BstFNI CGCG 2 cut(s) 132, 555
BstKTI GATC 5 cut(s) 115, 435, 532, 686, 785
BstMAI GTCTC 4 cut(s) 28, 162, 352, 755
BstMBI GATC 5 cut(s) 112, 432, 529, 683, 782
BstMWI GCNNNNNNNGC 9 cut(s) 71, 138, 147, 171, 180, 203, 389, 398, 737
BstNSI RCATGY 1 cut(s) 768
BstSFI CTRYAG 2 cut(s) 735, 838
BstUI CGCG 2 cut(s) 132, 555
BstV1I GCAGC 2 cut(s) 190, 743
BsuRI GGCC 2 cut(s) 187, 716
BtsCI GGATG 1 cut(s) 226
BtsIMutI CAGTG 3 cut(s) 414, 495, 673
Cac8I GCNNGC 6 cut(s) 130, 134, 152, 167, 185, 729
Cfr10I RCCGGY 2 cut(s) 165, 183
Csp6I GTAC 1 cut(s) 848
CviAII CATG 4 cut(s) 563, 601, 765, 786
CviQI GTAC 1 cut(s) 848
DdeI CTNAG 6 cut(s) 161, 375, 417, 483, 755, 777
DpnI GATC 5 cut(s) 114, 434, 531, 685, 784
DpnII GATC 5 cut(s) 112, 432, 529, 683, 782
DraI TTTAAA 1 cut(s) 333
DrdI GACNNNNNNGTC 2 cut(s) 16, 563
DseDI GACNNNNNNGTC 2 cut(s) 16, 563
EaeI YGGCCR 1 cut(s) 185
EciI GGCGGA 1 cut(s) 184
Eco130I CCWWGG 3 cut(s) 384, 643, 811
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 28
Eco32I GATATC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 475
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 384, 643, 811
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 3 cut(s) 384, 643, 811
FaeI CATG 4 cut(s) 566, 604, 768, 789
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FatI CATG 4 cut(s) 562, 600, 764, 785
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 6 cut(s) 66, 69, 72, 204, 468, 732
FokI GGATG 1 cut(s) 213
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 6 cut(s) 66, 69, 72, 204, 468, 732
FspBI CTAG 1 cut(s) 45
GluI GCNGC 6 cut(s) 66, 69, 72, 204, 468, 732
HaeIII GGCC 2 cut(s) 187, 716
HapII CCGG 3 cut(s) 86, 166, 184
Hin1I GRCGYC 1 cut(s) 540
Hin1II CATG 4 cut(s) 566, 604, 768, 789
HindIII AAGCTT 2 cut(s) 291, 327
HinfI GANTC 6 cut(s) 82, 218, 281, 701, 790, 872
HpaII CCGG 3 cut(s) 86, 166, 184
Hpy166II GTNNAC 1 cut(s) 860
Hpy188I TCNGA 5 cut(s) 162, 286, 534, 756, 780
Hpy188III TCNNGA 2 cut(s) 58, 230
Hpy8I GTNNAC 1 cut(s) 860
Hpy99I CGWCG 4 cut(s) 79, 82, 125, 203
HpyAV CCTTC 5 cut(s) 55, 241, 437, 487, 607
HpyCH4IV ACGT 2 cut(s) 11, 540
HpyCH4V TGCA 2 cut(s) 16, 340
HpyF10VI GCNNNNNNNGC 9 cut(s) 71, 138, 147, 171, 180, 203, 389, 398, 737
HpyF3I CTNAG 6 cut(s) 161, 375, 417, 483, 755, 777
HpySE526I ACGT 2 cut(s) 11, 540
Hsp92I GRCGYC 1 cut(s) 540
Hsp92II CATG 4 cut(s) 566, 604, 768, 789
KroI GCCGGC 2 cut(s) 165, 183
KroNI GCCGGC 2 cut(s) 167, 185
Kzo9I GATC 5 cut(s) 112, 432, 529, 683, 782
LmnI GCTCC 1 cut(s) 171
Lsp1109I GCAGC 2 cut(s) 190, 743
LweI GCATC 2 cut(s) 235, 327
MaeI CTAG 1 cut(s) 45
MaeII ACGT 2 cut(s) 11, 540
MaeIII GTNAC 3 cut(s) 7, 541, 604
MalI GATC 5 cut(s) 114, 434, 531, 685, 784
MboI GATC 5 cut(s) 112, 432, 529, 683, 782
MboII GAAGA 2 cut(s) 290, 299
MfeI CAATTG 1 cut(s) 189
MhlI GDGCHC 2 cut(s) 130, 138
MluCI AATT 6 cut(s) 189, 475, 498, 514, 624, 747
MlyI GAGTC 1 cut(s) 76
MnlI CCTC 5 cut(s) 217, 226, 232, 750, 809
MroNI GCCGGC 2 cut(s) 165, 183
MroXI GAANNNNTTC 1 cut(s) 705
MseI TTAA 2 cut(s) 32, 332
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 3 cut(s) 86, 166, 184
MunI CAATTG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 651
MvnI CGCG 2 cut(s) 132, 555
MwoI GCNNNNNNNGC 9 cut(s) 71, 138, 147, 171, 180, 203, 389, 398, 737
NaeI GCCGGC 2 cut(s) 167, 185
NdeII GATC 5 cut(s) 112, 432, 529, 683, 782
NgoMIV GCCGGC 2 cut(s) 165, 183
NlaIII CATG 4 cut(s) 566, 604, 768, 789
NlaIV GGNNCC 1 cut(s) 182
NmeAIII GCCGAG 2 cut(s) 82, 117
NmuCI GTSAC 2 cut(s) 7, 541
NspI RCATGY 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 651
PdiI GCCGGC 2 cut(s) 167, 185
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 5 cut(s) 218, 281, 701, 790, 872
PkrI GCNGC 6 cut(s) 67, 70, 73, 205, 469, 733
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 182
RsaI GTAC 1 cut(s) 849
RsaNI GTAC 1 cut(s) 848
SaqAI TTAA 2 cut(s) 32, 332
SatI GCNGC 6 cut(s) 66, 69, 72, 204, 468, 732
Sau3AI GATC 5 cut(s) 112, 432, 529, 683, 782
SchI GAGTC 1 cut(s) 76
SduI GDGCHC 2 cut(s) 130, 138
SfaNI GCATC 2 cut(s) 235, 327
SfcI CTRYAG 2 cut(s) 735, 838
Sse9I AATT 6 cut(s) 189, 475, 498, 514, 624, 747
SsiI CCGC 9 cut(s) 65, 68, 71, 106, 132, 169, 468, 553, 618
SspMI CTAG 1 cut(s) 45
StyI CCWWGG 3 cut(s) 384, 643, 811
TaiI ACGT 2 cut(s) 14, 543
TaqI TCGA 3 cut(s) 111, 221, 875
TaqII GACCGA 1 cut(s) 10
TasI AATT 6 cut(s) 189, 475, 498, 514, 624, 747
TatI WGTACW 1 cut(s) 847
TauI GCSGC 4 cut(s) 68, 71, 74, 470
TfiI GAWTC 5 cut(s) 218, 281, 701, 790, 872
Tru1I TTAA 2 cut(s) 32, 332
Tru9I TTAA 2 cut(s) 32, 332
TscAI CASTG 3 cut(s) 421, 495, 673
TseFI GTSAC 2 cut(s) 7, 541
TseI GCWGC 2 cut(s) 203, 731
Tsp45I GTSAC 2 cut(s) 7, 541
TspDTI ATGAA 4 cut(s) 231, 507, 551, 589
TspRI CASTG 3 cut(s) 421, 495, 673
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 768
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 1 cut(s) 45
ZraI GACGTC 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.