Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009460

Physical Location & Seq

Genomic Coordinates Forward (+)

27047604 .. 27050959

Gene Structure 3356 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1188700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 966 bp

ATGAGTGTGACATTGCAGTCCTTCTCGCTCTTAAACCCTAACCCTAGCAGCTGTGGATTGAAGAAGTCACCTTCTTCGTCTTCTTCTCTGCGGTTCGGACAATTGATTGCTGCTCGCGGTCATGGAGCCTCGCCTATTGCCCTCCCGAAAGGGCTTTCCGGCGCTTCTCTCTCCCTGCATCGGCGGAGCTTGTGGACTCGGCCGATTGTCGCCGCCGCTGCTTCTCAGGAGGAATCTCATTCAGACATTGAGGTGGAGAATGAAAAGAACAATGCTAAATCGGAATCTGAAGAATCAGAAGAAGCTTGGCAACAGACTCTGGCTTCTTTCAAAGAACAAGCCTTGAAGATGCAAAGCATCTCGCAGGAAGCATATGAGCAATACTCAAAGAAAGCATTGGTTATTATGAAAGAAACTTCGGAGCTGTTAAAAATACAGGCTGATAAGGCAAGGGATGACTTGAGTGAGATAGCAAAAGAAATCAGTGAAGAAGGTAAAGAATATATCTCCAAAGCTGCAAAGAATTCCCCTGAGCCAGTGAAGGAAATTGTTGAAACATATAATTCATCAGCTGATGATCTCAGTGATGTTTCACAAGTCCGCGACTTTCATGTTGGGATACCTTATGGTCTTTTGCTTTCTGTTGGTGGATTCCTGTCCTTCATGGTAACGGGAAGCACTTCTGCAATTAGGTTCGGGGTTATACTTGGTGCCACTCTTTTGTTTCTAAGTGTTTCAAGTTTAAGATCATACAAAGGAGGAGAATCATCTCCTTTGGTCTTGAAAGGGCAGACAGCTATATCCAGTATAATTTTTCTGAGGGAGCTACGCTTTCTGGCTCAGAGATCATCGTTTCCGAATTTTTTAACAACCCTTGTCAGTGGTGCAGTTGTAGCTTTCTACTTCTATAGGATTGTACAGAATCGCAAACATCAAAAGGGTTCCAACTTTGAGAAGGAGACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009706 GO:0009941 GO:0016020 GO:0019866 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tmemb_14

Protein Analysis

322

Amino Acids

35.09

Weight (kDa)

7.78

Isoelectric Point (pI)

51.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tmemb_14	PF03647	205 - 301	5.9e-15	Transmembrane proteins 14C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38550
fragaria_vesca	FvH4_6g09820 FvH4_6g09820 FvH4_6g09820 FvH4_6g09820
malus_domestica	MD04G1175000.v1.1 MD12G1188700.v1.1
prunus_persica	Prupe.6G294000_v2.0.a1 Prupe.6G294000_v2.0.a1
pyrus_communis	pycom04g15440
rosa_chinensis	RchiOBHm_Chr3g0460431
rosa_laevigata	RLG00000025012
rosa_multiflora	Rmu_sc0004267.1_g000011 Rmu_sc0021028.1_g000002
rosa_roxburghii	Rroxscaffold_6G00419490
rosa_rugosa	Rorug03G0042600 Rorug03G0042600
rosa_samantha	Rh3AG100600 Rh3BG104600 Rh3CG106400 Rh3DG105900
rosa_wichuraiana	Rw3G008560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	16
AccB1I	GGYRCC	1 cut(s)	710
AccII	CGCG	2 cut(s)	117, 603
AciI	CCGC	6 cut(s)	91, 117, 184, 213, 216, 601
AcoI	YGGCCR	1 cut(s)	200
AcsI	RAATTY	2 cut(s)	523, 859
AcuI	CTGAAG	1 cut(s)	309
AfaI	GTAC	1 cut(s)	918
AfiI	CCNNNNNNNGG	1 cut(s)	180
AgsI	TTSAA	6 cut(s)	61, 331, 346, 554, 738, 784
AluBI	AGCT	9 cut(s)	51, 189, 305, 424, 515, 572, 797, 826, 896
AluI	AGCT	9 cut(s)	51, 189, 305, 424, 515, 572, 797, 826, 896
Alw26I	GTCTC	1 cut(s)	953
AlwNI	CAGNNNCTG	1 cut(s)	319
AoxI	GGCC	1 cut(s)	200
ApeKI	GCWGC	4 cut(s)	48, 110, 218, 515
ApoI	RAATTY	2 cut(s)	523, 859
Asp700I	GAANNNNTTC	1 cut(s)	679
AspLEI	GCGC	1 cut(s)	164
AsuHPI	GGTGA	1 cut(s)	60
BanI	GGYRCC	1 cut(s)	710
BbsI	GAAGAC	1 cut(s)	72
BbvI	GCAGC	4 cut(s)	60, 97, 205, 502
BciVI	GTATCC	1 cut(s)	612
BcoDI	GTCTC	1 cut(s)	953
BfaI	CTAG	1 cut(s)	45
BfmI	CTRYAG	1 cut(s)	907
BfoI	RGCGCY	1 cut(s)	165
BfuI	GTATCC	1 cut(s)	612
BisI	GCNGC	6 cut(s)	49, 111, 213, 216, 219, 516
BlsI	GCNGC	6 cut(s)	50, 112, 214, 217, 220, 517
BmiI	GGNNCC	3 cut(s)	127, 712, 943
BmsI	GCATC	3 cut(s)	187, 339, 366
BpiI	GAAGAC	1 cut(s)	72
BplI	GAGNNNNNCTC	2 cut(s)	368, 400
Bpu10I	CCTNAGC	1 cut(s)	531
BpuEI	CTTGAG	1 cut(s)	481
Bsc4I	CCNNNNNNNGG	1 cut(s)	180
Bse1I	ACTGG	2 cut(s)	536, 804
Bse3DI	GCAATG	1 cut(s)	11
BseGI	GGATG	1 cut(s)	460
BseLI	CCNNNNNNNGG	1 cut(s)	180
BseMI	GCAATG	1 cut(s)	11
BseMII	CTCAG	5 cut(s)	239, 522, 595, 809, 854
BseNI	ACTGG	2 cut(s)	536, 804
BseRI	GAGGAG	1 cut(s)	774
BseX3I	CGGCCG	1 cut(s)	200
BseXI	GCAGC	4 cut(s)	60, 97, 205, 502
BsgI	GTGCAG	1 cut(s)	906
Bsh1236I	CGCG	2 cut(s)	117, 603
Bsh1285I	CGRYCG	1 cut(s)	203
BshFI	GGCC	1 cut(s)	202
BshNI	GGYRCC	1 cut(s)	710
BsiEI	CGRYCG	1 cut(s)	203
BsiSI	CCGG	1 cut(s)	159
BslI	CCNNNNNNNGG	1 cut(s)	180
BsmAI	GTCTC	1 cut(s)	953
BsmBI	CGTCTC	1 cut(s)	953
BsnI	GGCC	1 cut(s)	202
Bsp1407I	TGTACA	1 cut(s)	916
Bsp143I	GATC	3 cut(s)	577, 746, 845
BspACI	CCGC	6 cut(s)	91, 117, 184, 213, 216, 601
BspANI	GGCC	1 cut(s)	202
BspCNI	CTCAG	5 cut(s)	238, 523, 594, 810, 853
BspFNI	CGCG	2 cut(s)	117, 603
BspLI	GGNNCC	3 cut(s)	127, 712, 943
BspT107I	GGYRCC	1 cut(s)	710
BsrDI	GCAATG	1 cut(s)	11
BsrGI	TGTACA	1 cut(s)	916
BsrI	ACTGG	2 cut(s)	536, 804
BssMI	GATC	3 cut(s)	577, 746, 845
BstAUI	TGTACA	1 cut(s)	916
BstC8I	GCNNGC	1 cut(s)	115
BstDEI	CTNAG	6 cut(s)	225, 531, 581, 728, 818, 840
BstF5I	GGATG	1 cut(s)	460
BstFNI	CGCG	2 cut(s)	117, 603
BstH2I	RGCGCY	1 cut(s)	165
BstHHI	GCGC	1 cut(s)	164
BstKTI	GATC	3 cut(s)	580, 749, 848
BstMAI	GTCTC	1 cut(s)	953
BstMBI	GATC	3 cut(s)	577, 746, 845
BstMCI	CGRYCG	1 cut(s)	203
BstMWI	GCNNNNNNNGC	3 cut(s)	218, 446, 893
BstSFI	CTRYAG	1 cut(s)	907
BstUI	CGCG	2 cut(s)	117, 603
BstV1I	GCAGC	4 cut(s)	60, 97, 205, 502
BstV2I	GAAGAC	1 cut(s)	72
BstZI	CGGCCG	1 cut(s)	200
BsuI	GTATCC	1 cut(s)	612
BsuRI	GGCC	1 cut(s)	202
BtsCI	GGATG	1 cut(s)	460
BtsIMutI	CAGTG	4 cut(s)	490, 543, 589, 886
Cac8I	GCNNGC	1 cut(s)	115
CaiI	CAGNNNCTG	1 cut(s)	319
CfoI	GCGC	1 cut(s)	164
Csp6I	GTAC	1 cut(s)	917
CviAII	CATG	3 cut(s)	122, 611, 664
CviQI	GTAC	1 cut(s)	917
DdeI	CTNAG	6 cut(s)	225, 531, 581, 728, 818, 840
DpnI	GATC	3 cut(s)	579, 748, 847
DpnII	GATC	3 cut(s)	577, 746, 845
DrdI	GACNNNNNNGTC	1 cut(s)	16
DseDI	GACNNNNNNGTC	1 cut(s)	16
EaeI	YGGCCR	1 cut(s)	200
EagI	CGGCCG	1 cut(s)	200
EciI	GGCGGA	1 cut(s)	199
EclXI	CGGCCG	1 cut(s)	200
Eco52I	CGGCCG	1 cut(s)	200
Eco57I	CTGAAG	1 cut(s)	309
EcoRI	GAATTC	1 cut(s)	523
Esp3I	CGTCTC	1 cut(s)	953
FaeI	CATG	3 cut(s)	125, 614, 667
FatI	CATG	3 cut(s)	121, 610, 663
FauNDI	CATATG	1 cut(s)	373
Fnu4HI	GCNGC	6 cut(s)	49, 111, 213, 216, 219, 516
FokI	GGATG	1 cut(s)	467
Fsp4HI	GCNGC	6 cut(s)	49, 111, 213, 216, 219, 516
FspBI	CTAG	1 cut(s)	45
GlaI	GCGC	1 cut(s)	163
GluI	GCNGC	6 cut(s)	49, 111, 213, 216, 219, 516
HaeII	RGCGCY	1 cut(s)	165
HaeIII	GGCC	1 cut(s)	202
HapII	CCGG	1 cut(s)	159
HhaI	GCGC	1 cut(s)	164
Hin1II	CATG	3 cut(s)	125, 614, 667
Hin6I	GCGC	1 cut(s)	162
HinP1I	GCGC	1 cut(s)	162
HindIII	AAGCTT	1 cut(s)	303
HinfI	GANTC	8 cut(s)	196, 233, 284, 293, 316, 651, 764, 922
HpaII	CCGG	1 cut(s)	159
HphI	GGTGA	1 cut(s)	60
Hpy166II	GTNNAC	1 cut(s)	195
Hpy188I	TCNGA	9 cut(s)	98, 244, 283, 289, 298, 421, 819, 843, 858
Hpy188III	TCNNGA	3 cut(s)	145, 227, 781
Hpy8I	GTNNAC	1 cut(s)	195
HpyAV	CCTTC	6 cut(s)	31, 81, 485, 535, 670, 949
HpyCH4IV	ACGT	1 cut(s)	962
HpyCH4V	TGCA	6 cut(s)	16, 178, 352, 518, 686, 887
HpyF10VI	GCNNNNNNNGC	3 cut(s)	218, 446, 893
HpyF3I	CTNAG	6 cut(s)	225, 531, 581, 728, 818, 840
HpySE526I	ACGT	1 cut(s)	962
Hsp92II	CATG	3 cut(s)	125, 614, 667
HspAI	GCGC	1 cut(s)	162
Kzo9I	GATC	3 cut(s)	577, 746, 845
LmnI	GCTCC	4 cut(s)	125, 186, 421, 823
Lsp1109I	GCAGC	4 cut(s)	60, 97, 205, 502
LweI	GCATC	3 cut(s)	187, 339, 366
MaeI	CTAG	1 cut(s)	45
MaeII	ACGT	1 cut(s)	962
MaeIII	GTNAC	3 cut(s)	7, 66, 667
MalI	GATC	3 cut(s)	579, 748, 847
MboI	GATC	3 cut(s)	577, 746, 845
MboII	GAAGA	8 cut(s)	66, 72, 73, 75, 302, 311, 358, 500
MfeI	CAATTG	1 cut(s)	101
MluCI	AATT	7 cut(s)	101, 523, 546, 562, 687, 810, 859
MlyI	GAGTC	2 cut(s)	190, 310
MnlI	CCTC	6 cut(s)	139, 152, 223, 244, 752, 813
MroXI	GAANNNNTTC	1 cut(s)	679
MseI	TTAA	4 cut(s)	32, 428, 743, 866
MslI	CAYNNNNRTG	1 cut(s)	251
MspA1I	CMGCKG	3 cut(s)	51, 218, 572
MspI	CCGG	1 cut(s)	159
MunI	CAATTG	1 cut(s)	101
MvnI	CGCG	2 cut(s)	117, 603
MwoI	GCNNNNNNNGC	3 cut(s)	218, 446, 893
NdeI	CATATG	1 cut(s)	373
NdeII	GATC	3 cut(s)	577, 746, 845
NlaIII	CATG	3 cut(s)	125, 614, 667
NlaIV	GGNNCC	3 cut(s)	127, 712, 943
NmeAIII	GCCGAG	1 cut(s)	178
NmuCI	GTSAC	2 cut(s)	7, 66
PdmI	GAANNNNTTC	1 cut(s)	679
PfeI	GAWTC	6 cut(s)	233, 284, 293, 651, 764, 922
PkrI	GCNGC	6 cut(s)	50, 112, 214, 217, 220, 517
PleI	GAGTC	2 cut(s)	190, 310
PpsI	GAGTC	2 cut(s)	190, 310
PspN4I	GGNNCC	3 cut(s)	127, 712, 943
PstNI	CAGNNNCTG	1 cut(s)	319
PvuII	CAGCTG	2 cut(s)	51, 572
RsaI	GTAC	1 cut(s)	918
RsaNI	GTAC	1 cut(s)	917
RseI	CAYNNNNRTG	1 cut(s)	251
SaqAI	TTAA	4 cut(s)	32, 428, 743, 866
SatI	GCNGC	6 cut(s)	49, 111, 213, 216, 219, 516
Sau3AI	GATC	3 cut(s)	577, 746, 845
SchI	GAGTC	2 cut(s)	190, 310
SfaNI	GCATC	3 cut(s)	187, 339, 366
SfcI	CTRYAG	1 cut(s)	907
SmiMI	CAYNNNNRTG	1 cut(s)	251
SmlI	CTYRAG	1 cut(s)	460
SmoI	CTYRAG	1 cut(s)	460
Sse9I	AATT	7 cut(s)	101, 523, 546, 562, 687, 810, 859
SsiI	CCGC	6 cut(s)	91, 117, 184, 213, 216, 601
SspMI	CTAG	1 cut(s)	45
TaiI	ACGT	1 cut(s)	965
TasI	AATT	7 cut(s)	101, 523, 546, 562, 687, 810, 859
TatI	WGTACW	1 cut(s)	916
TauI	GCSGC	2 cut(s)	215, 218
TfiI	GAWTC	6 cut(s)	233, 284, 293, 651, 764, 922
Tru1I	TTAA	4 cut(s)	32, 428, 743, 866
Tru9I	TTAA	4 cut(s)	32, 428, 743, 866
TscAI	CASTG	4 cut(s)	490, 543, 589, 886
TseFI	GTSAC	2 cut(s)	7, 66
TseI	GCWGC	4 cut(s)	48, 110, 218, 515
Tsp45I	GTSAC	2 cut(s)	7, 66
TspDTI	ATGAA	5 cut(s)	276, 422, 555, 599, 652
TspRI	CASTG	4 cut(s)	490, 543, 589, 886
XapI	RAATTY	2 cut(s)	523, 859
XmnI	GAANNNNTTC	1 cut(s)	679
XspI	CTAG	1 cut(s)	45

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.