AT2G40770

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003903
Physical Location & Seq
Reverse (-)
17013269 .. 17021606
8338 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40770.3

Sequence Viewer

Length: 4995 bp
ATGGGTAGGAGAAAGCAATCCAAGCCCCAGCGTGCAGTGGGTTTGATCACTCCAACTGGTTCTGAATCCGATAGAAATCAATTACCCGGCGACGAAGCTGAGGGTTCAGGGGACAAGAATGTTGAAGATATCGATAAGCCGTATTATGTGAACATATGTTTAAGTAGTCGAGTTTCGGAGCAGCAGCAGAACTTTGATTTAGCTGAAGTTGTTCTAACGAATTTTAGTGTAAGAGAACGTGTAGGAAGTAGTAGTACATTCACAAAACCTATTGAGGTTGATCATGATCTGGATTGCTCGTTACGGTTTCGGCTTTGCAATGTGACTAATTTTGTTGATCGGATTAAGCTTGGTCACTGGCCGGTTTTGTCTTCGAGTGATATAACTTTGGAATTGGTTGATAATAAGGTGTCTGATGATGAGGTTGGGTCAGTGATTTGGTCTGCAAGTTTTGATGGTCCAGGTGAAGGGGTTTCGGGTCTTGCTCATTTGGCAAGTATAAAGTTTTTGACTTTAAGGCTCATGCCTGGTAATGAGGGATTGTTGTCTCCTAGAGTTAGAGTTGAGATGCTTCAGCAGGCTTTTGATGCATGTGATTCTCTTCTTGAGAATACAAGGCAGATTTGGAAAAAGAGTATGATTCATGTCATGTCTTGGTTGAGGCCAGAGGTAATGACATCAGAGGCTAGGTATGGGACAAGATTCAACGTCAAGGATATTGAGTCATCAGTTGTGACGGAAGATGAAACTTTGGACTCTAGTAAGCAGTCTAGTTTTGATGCTGCTGCTTTTTATGAAGCCATAAAGCCATCAAAGACGGATGCAATGCTTGGAGATGACATAGCTGATTTGCTCCCAGAACTTAGGCCGTACCAACGACGGGCAGCTTATTGGATGGTTCAGCGAGAGAGAGGCGATCCAATAACGTTGGGAGACAAGGAAGACAACCAATTCATTTCACCTTTGTCTATCTCCGTGGGATTCCTTGACTCTGCTACAAAAATGTTCTTGAACCCATTCAGTGGGAATATCTCATTGACACCAGAGTATTTTTCACCTCGAATTCAGGGCGGTATCCTTGCTGATGAAATGGGATTGGGAAAAACGGTGGAACTACTTGCTTGTATCTTTTCTCATCGAAAACCAGCGGAGGATGAAATTTCTGTGTCTAATGGTTCATCAGTTACTGATGTTTTGAACGCTGGCTTGAGAAGATTGAAAAGAGAACGTGTTGAGTGCATATGTGGAGCTGTCAGTGAAAGTCACAAATACAAAGGAGTTTGGGTACAGTGTGACCTTTGTGATGCTTGGCAGCATGCCGACTGTGTAGGTTATTCACCTAAAGGGAAAGGTAAGAAGGATAGTCAACATATTGATGAAAAAGCATCTCAAAAGAAGAGCAAAAAGGATGCAACGGAAATTATTGTCAGAGAAGGTGAATATATTTGCCAAATGTGCTCTGAACTTCTACAAGTCACTGCCTCTCCCATCTCTACGGGTGCCACTCTTATTGTCTGTCCAGCTCCTATATTACCGCAATGGCATTCCGAGATTACACGCCATACACGCTTGGGATCTCTCATAACATGTATCTATGAAGGTGTGAGGAACGCCTCACTTTCTGAAGAACCTATGATTGACATTACTGAACTTCTCAATGCGGACATTGTTTTAACTACGTATGATGTCCTTAAAGAGGACTTGACACATGACTTCGACAGGCATGATGGTGACCGGCACTGTCTTAGATTCCAGAAAAGGTACCCTGTTATTCCGACTCCACTCACCAGAATATTTTGGTGGAGGATTTGTTTGGATGAGGCCCAGATGGTGGAGAGCAATGCAGCTGCTGCAACAGAGATGGCGTTGAGATTATACACCAAACACCGTTGGTGTATCACCGGAACTCCTATACAACGCAAACTTGATGACTTATTTGGACTATTGAAGTTTCTTAAAGCAAATCCTTTCGATGTTTCAAGATGGTGGATTGAAGTTATAAGAGACCCATATGAGAGGCGAGACACAAAGGCCATGGAATTTACGCATAAGTTTTTCAAACAAGTTATGTGGCGTTCTTCAAAAGTCCATGTGGCTGATGAGTTGCAACTTCCACCTCAAGAGGAATGCGTCTCGTGGCTTAAATTTTCCGCAATTGAAGAACACTTTTACAGTAGACAGCATGACACTTGTGTGAGTTATGCTCGTGAAGTTATAGAAACTTTGAAACGTGATATTCTCAAAAGAGGTCATACTTCTTCTGATAATCCTTTAGTCACTCATGCTGAAGCTGCAAAACTGTTGAACTCGCTCTTGAAATTGCGCCAAGCTTGCTGCCACCCTCAAGTAGGAAGTTCAGGTTTACGCTCTTTGCAACAAAGTCCAATGACCATGGAAGAAATTTTAATGGTCCTTGTGAAAAAGACCCAGAGCGAGGGAGAAGAAGCTCTTAGAGTGTTAATTGTTGCATTAAATGGGATTGCTGCTATTGCCATGCTTAAGCAAGAATTTTCTGAAGCTGTGTCCTTATACAAGGAAGCATTAAGTATAACTGAAGAACACGCCGAGGATTTTCGTCTTGATCCATTGTTAAATATTCACATTCTTCACAATTTAGCTGAGATACTACCAATGGCCAAAAGCTACGGTGGAAAACTTTCTGCATCAGGAAGGCCTGAAACAAAAATAGATGTAAAAGATGATGATCATCATCGTGCATCGAAGAGGCAAAGGATCAATGAGCTCGAGAGTTTAACTCATGATTCTTCAGAAACTGTACATCAGCGGGAAGCTATTGCTCCAGACAATGGTTTAAAGAAGGATGGAGAGTGTCATGAAGAATGCAAAACTCTGGATATAGTTTGTGACACACTGAAGGTTAAGTATTTATCCGCATTCAACTCAAAGCTTTCTGCAGCACAGCACGAATTCAAAAAATCATACAATCAGGTTTCTGAGTCGTTGAGCAATATGGGGAAACAACGTTCAGTTTGGTGGTTGGATGCCCTGCAACTTACTGAACAGAATAAAGACTTCTCTAGCGAGTTGACAAGGAAGATTGAAGAGGCCCTCCATGGAAACTTAAACAATTCAAGCTCCTCCAGAGAGAGTTCTCGATTTAGAACTATACATGGCATGAAACTTCATCTGCAGACGTGTATGGATATGCTGGAGAGATCTAGAAAGAAAGTGATAGATAGGATTCTAGAAATTGATCAGACTATGGAGAAACCAAAGTTGGAGGATATTGAGCGTATTAGCAACTGCAAGTATTGCAATAAGAATAGTGATGGCCCCCCGTGTATTCATTGCGAGCTAGATGAACTATTCCAGGAATATGAGGCTAGGCTTTTCCGTCTTAACAAATCTCGCAGGGGAGTAATGGAAATTGCGGCTGCTGAAGAGACGGTACATTTGCAGAAGAAGAGAGATGCTCGTAATCTTTTTTTATTTGGTTTATCATCAAGAAGCAAGGATCTAAATGCATCACGTGGTGATGACGAGGAGCCAACCAAAAGAAATGCTGGTGACATTGTGGTTCTTTCAAAATCTCCATCTGAGACGGAAATAGTTCTTGGAGTGATAAGAAACCATTGCAAAACTCATTTAGATAGGGAAAGCAAATTGGCAGCTACAAAACATTTGCATACCCTTGAGGTGATGCGAAAGGAATATGTACATGCAAGGGTCTTAGCTAGGGATCAAGCTCAACTCCTACGTGCCTATGATGAGATTAACATGTCAACAATGAGACTACAGCTTAGAGAATCGGAAGATGACACATCGATCTATGCTTTGGGCCGGGACGAGTTAGATGTTGCTAGTGTGCTGAACACAAATGACAAATTTATGGCTCAATCCTCGTTGCTTAGTATAAAAGGGAAACTTCGTTATTTGAAGGGTTTGATGAAATCCAAACAGAAACAAGAATCCGAGAGTCCAGATCTTTCCTCACCAATACATGAAACAGTCGATGCTTCGGATCCTGCTGAACAGGAAAGCGAAAACCTGCTTAAGAGAGATGAAGCATGCCCGATTTGTCATGAAATTCTAAGGAATCAGAAGATGGTTTTCCAGTGCGGGCATTCCACTTGCTGTAACTGCTTTTTTGCTATGACTGAGCGCAAATCAGTTCAAGAAACGCTGCAAAAGTGGGTGATGTGTCCAATATGCAGGCAGCATACAGATGTTAGAAATATTGCATATGCTGATGATAGAAGAAACAGTTCTTCGTCAGATCAAGATCACAAAGACAGTGAAGCATCATTGGTGGTTCAAGGTTCATATGGAACAAAGATTGAAGCTGTTACTAGAAGAATCTTATGGATCAAGTCAAGTGACCCACAAACGAAGGTTCTTGTTTTCTCCAGCTGGAACGATGTTCTTGATGTGTTGGAACATGCCTTCGCTGCAAATAGCATCACCTGCATCCGGATGAAAGGGGGCAGGAAATCACAGACAGCCATTAGCAAATTCAAGGGATCTGAGAAAGAAACCCAAAAGACAAACTCCCATCAAAAAGAGGAAAAATCGATTCAAGTGTTATTGCTTTTGGTGCAGCATGGTGCCAACGGTCTTAATCTTCTAGAAGCACAGCATGTTATTCTGGTGGAGCCACTTCTAAATCCAGCTGCGGAAGCGCAAGCTGTTGGGCGTGTTCACCGAATTGGACAAGAGAAGCCTACTCTAGTTCATCGGTTTCTGGTATCGGGCACAGTGGAAGAGAGCATCTACAAACTGAACAGGAATAAGAATACAAACTTAAGTTCATTTAGCAGCAGGAATACAAAGAATCAAGATCAACAGTTCTTGACACTAAAAGATCTTGAATCTCTATTTGCTTCACCAACAGCAGAAACAGCAGAGATGGAACAAAACCCAGGAGAGAGACAGGAGAATCTGAGAGATCTACCACCCTCAGTCGCTGCAGCATTAGCAGCCGAGAGGAGAATGAAGGAAAGTAATGCATCCTCATCAACTACAAACGCATCATGA

Protein Analysis

1664

Amino Acids

188.07

Weight (kDa)

6.17

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 291 - 782 4.5e-67 SNF2-related domain
SHPRH_helical-1st PF21325 798 - 879 1.5e-24 E3 ubiquitin-protein ligase SHPRH, first helical domain
SHPRH_helical-2nd PF21324 990 - 1138 5.2e-09 E3 ubiquitin-protein ligase SHPRH, second helical domain
Helicase_C PF00271 1434 - 1557 8.5e-10 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2000
AarI CACCTGC 1 cut(s) 4433
Acc36I ACCTGC 2 cut(s) 4017, 4433
Acc65I GGTACC 1 cut(s) 1761
AccB1I GGYRCC 3 cut(s) 1499, 1761, 4565
AccB7I CCANNNNNTGG 3 cut(s) 1022, 1831, 2807
AccI GTMKAC 1 cut(s) 2176
AccIII TCCGGA 1 cut(s) 4431
AclI AACGTT 2 cut(s) 926, 2983
AcoI YGGCCR 2 cut(s) 359, 2634
AcuI CTGAAG 9 cut(s) 225, 557, 1644, 2307, 2535, 2574, 2751, 2894, 3420
AcvI CACGTG 1 cut(s) 3491
AdeI CACNNNGTG 1 cut(s) 3494
AfaI GTAC 7 cut(s) 256, 872, 1287, 1763, 2778, 3411, 3678
AfiI CCNNNNNNNGG 9 cut(s) 467, 880, 1022, 1696, 1831, 2348, 2434, 2807, 4431
AflII CTTAAG 3 cut(s) 2498, 4013, 4762
AflIII ACRYGT 5 cut(s) 238, 1228, 1586, 3155, 3738
AjiI CACGTC 1 cut(s) 3156
AjnI CCWGG 4 cut(s) 460, 526, 3330, 4879
AjuI GAANNNNNNNTTGG 4 cut(s) 3221, 3253, 3558, 3590
AleI CACNNNNGTG 1 cut(s) 2192
AloI GAACNNNNNNTCC 2 cut(s) 2968, 3000
Alw21I GWGCWC 2 cut(s) 1460, 2745
Alw26I GTCTC 9 cut(s) 552, 927, 1998, 2016, 2137, 3398, 3554, 3745, 4882
AlwNI CAGNNNCTG 4 cut(s) 1187, 1850, 2774, 4925
Ama87I CYCGRG 1 cut(s) 2744
Aor13HI TCCGGA 1 cut(s) 4431
ArsI GACNNNNNNTTYG 2 cut(s) 3397, 3429
Asp700I GAANNNNTTC 5 cut(s) 210, 1016, 2656, 3570, 4225
Asp718I GGTACC 1 cut(s) 1761
AspLEI GCGC 3 cut(s) 2325, 4125, 4642
AspS9I GGNCC 6 cut(s) 458, 1822, 2410, 3067, 3293, 3799
AsuC2I CCSGG 2 cut(s) 87, 3803
AvaI CYCGRG 1 cut(s) 2744
AvaII GGWCC 2 cut(s) 458, 2410
BaeGI GKGCMC 1 cut(s) 4715
BalI TGGCCA 1 cut(s) 2636
BamHI GGATCC 1 cut(s) 3982
BanI GGYRCC 3 cut(s) 1499, 1761, 4565
BanII GRGCYC 1 cut(s) 2745
BarI GAAGNNNNNNTAC 4 cut(s) 238, 270, 3393, 3425
BauI CACGAG 2 cut(s) 2134, 2205
BbrPI CACGTG 1 cut(s) 3491
BbsI GAAGAC 2 cut(s) 363, 948
Bbv12I GWGCWC 2 cut(s) 1460, 2745
BbvCI CCTCAGC 1 cut(s) 99
BceAI ACGGC 2 cut(s) 124, 853
BciT130I CCWGG 4 cut(s) 462, 528, 3332, 4881
BciVI GTATCC 1 cut(s) 1085
BclI TGATCA 4 cut(s) 45, 280, 2704, 3214
BcnI CCSGG 2 cut(s) 87, 3803
BcoDI GTCTC 9 cut(s) 552, 927, 1998, 2016, 2137, 3398, 3554, 3745, 4882
BfmI CTRYAG 4 cut(s) 2913, 3149, 3755, 4926
BfrI CTTAAG 3 cut(s) 2498, 4013, 4762
BfuAI ACCTGC 2 cut(s) 4017, 4433
BfuI GTATCC 1 cut(s) 1085
BglII AGATCT 4 cut(s) 3176, 3943, 4822, 4906
Bme1390I CCNGG 6 cut(s) 87, 462, 528, 3332, 3803, 4881
Bme18I GGWCC 2 cut(s) 458, 2410
BmeT110I CYCGRG 1 cut(s) 2744
BmgBI CACGTC 1 cut(s) 3156
BmgT120I GGNCC 6 cut(s) 458, 1822, 2410, 3067, 3293, 3799
BmiI GGNNCC 7 cut(s) 1501, 1763, 3295, 3507, 3984, 4567, 4614
BmrFI CCNGG 6 cut(s) 87, 462, 528, 3332, 3803, 4881
BpiI GAAGAC 2 cut(s) 363, 948
BplI GAGNNNNNCTC 6 cut(s) 2188, 2220, 2740, 2772, 3418, 3450
BpmI CTGGAG 4 cut(s) 2784, 3085, 3191, 4351
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 5 cut(s) 626, 1228, 2103, 2328, 3674
BpuMI CCSGG 2 cut(s) 87, 3803
Bsa29I ATCGAT 3 cut(s) 132, 3785, 4532
BsaAI YACGTR 3 cut(s) 1680, 3491, 3719
BsaBI GATNNNNATC 6 cut(s) 75, 285, 2703, 2706, 2709, 4242
BsaI GGTCTC 1 cut(s) 1998
BsaJI CCNNGG 6 cut(s) 975, 2034, 2391, 2565, 3073, 4879
BsaWI WCCGGW 2 cut(s) 1901, 4431
BsaXI ACNNNNNCTCC 8 cut(s) 1269, 1299, 1468, 1498, 1795, 1825, 1891, 1921
Bsc4I CCNNNNNNNGG 9 cut(s) 467, 880, 1022, 1696, 1831, 2348, 2434, 2807, 4431
Bse118I RCCGGY 2 cut(s) 361, 1734
Bse1I ACTGG 3 cut(s) 61, 362, 4075
Bse3DI GCAATG 6 cut(s) 325, 831, 1544, 1846, 3307, 3592
Bse8I GATNNNNATC 6 cut(s) 75, 285, 2703, 2706, 2709, 4242
BseAI TCCGGA 1 cut(s) 4431
BseBI CCWGG 4 cut(s) 462, 528, 3332, 4881
BseCI ATCGAT 3 cut(s) 132, 3785, 4532
BseDI CCNNGG 6 cut(s) 975, 2034, 2391, 2565, 3073, 4879
BseJI GATNNNNATC 6 cut(s) 75, 285, 2703, 2706, 2709, 4242
BseLI CCNNNNNNNGG 9 cut(s) 467, 880, 1022, 1696, 1831, 2348, 2434, 2807, 4431
BseMI GCAATG 6 cut(s) 325, 831, 1544, 1846, 3307, 3592
BseMII CTCAG 8 cut(s) 90, 2610, 2946, 3549, 4110, 4476, 4892, 4932
BseNI ACTGG 3 cut(s) 61, 362, 4075
BseRI GAGGAG 3 cut(s) 3088, 3518, 4960
BseSI GKGCMC 1 cut(s) 4715
BseYI CCCAGC 1 cut(s) 27
BsgI GTGCAG 2 cut(s) 54, 4577
BshNI GGYRCC 3 cut(s) 1499, 1761, 4565
BshVI ATCGAT 3 cut(s) 132, 3785, 4532
BsiHKAI GWGCWC 2 cut(s) 1460, 2745
BsiHKCI CYCGRG 1 cut(s) 2744
BsiSI CCGG 6 cut(s) 87, 362, 1735, 1902, 3802, 4432
BslFI GGGAC 3 cut(s) 125, 709, 3818
BslI CCNNNNNNNGG 9 cut(s) 467, 880, 1022, 1696, 1831, 2348, 2434, 2807, 4431
BsmAI GTCTC 9 cut(s) 552, 927, 1998, 2016, 2137, 3398, 3554, 3745, 4882
BsmBI CGTCTC 3 cut(s) 2137, 3398, 3554
BsmFI GGGAC 3 cut(s) 125, 709, 3818
BsmI GAATGC 5 cut(s) 1543, 2132, 2846, 2894, 4084
Bso31I GGTCTC 1 cut(s) 1998
BsoBI CYCGRG 1 cut(s) 2744
Bsp1286I GDGCHC 3 cut(s) 1460, 2745, 4715
Bsp13I TCCGGA 1 cut(s) 4431
Bsp1407I TGTACA 2 cut(s) 2776, 3676
Bsp19I CCATGG 3 cut(s) 2034, 2391, 3073
BspCNI CTCAG 8 cut(s) 91, 2611, 2947, 3550, 4111, 4477, 4893, 4931
BspDI ATCGAT 3 cut(s) 132, 3785, 4532
BspEI TCCGGA 1 cut(s) 4431
BspHI TCATGA 5 cut(s) 283, 2758, 2833, 4042, 4991
BspLI GGNNCC 7 cut(s) 1501, 1763, 3295, 3507, 3984, 4567, 4614
BspMAI CTGCAG 3 cut(s) 2917, 3153, 4930
BspMI ACCTGC 2 cut(s) 4017, 4433
BspQI GCTCTTC 1 cut(s) 1391
BspT107I GGYRCC 3 cut(s) 1499, 1761, 4565
BspTI CTTAAG 3 cut(s) 2498, 4013, 4762
BspTNI GGTCTC 1 cut(s) 1998
BsrDI GCAATG 6 cut(s) 325, 831, 1544, 1846, 3307, 3592
BsrFI RCCGGY 2 cut(s) 361, 1734
BsrGI TGTACA 2 cut(s) 2776, 3676
BsrI ACTGG 3 cut(s) 61, 362, 4075
BssAI RCCGGY 2 cut(s) 361, 1734
BssECI CCNNGG 6 cut(s) 975, 2034, 2391, 2565, 3073, 4879
BssSI CACGAG 2 cut(s) 2134, 2205
BssT1I CCWWGG 3 cut(s) 2034, 2391, 3073
Bst2BI CACGAG 2 cut(s) 2134, 2205
Bst2UI CCWGG 4 cut(s) 462, 528, 3332, 4881
Bst6I CTCTTC 7 cut(s) 606, 1391, 2717, 3057, 3396, 3419, 4716
BstAFI CTTAAG 3 cut(s) 2498, 4013, 4762
BstAPI GCANNNNNTGC 3 cut(s) 1850, 3273, 4425
BstAUI TGTACA 2 cut(s) 2776, 3676
BstBAI YACGTR 3 cut(s) 1680, 3491, 3719
BstDSI CCRYGG 4 cut(s) 975, 2034, 2391, 3073
BstEII GGTNACC 1 cut(s) 1730
BstENI CCTNNNNNAGG 2 cut(s) 1694, 2346
BstHHI GCGC 3 cut(s) 2325, 4125, 4642
BstMAI GTCTC 9 cut(s) 552, 927, 1998, 2016, 2137, 3398, 3554, 3745, 4882
BstNI CCWGG 4 cut(s) 462, 528, 3332, 4881
BstNSI RCATGY 8 cut(s) 594, 1319, 1590, 3683, 3742, 4032, 4403, 4601
BstPI GGTNACC 1 cut(s) 1730
BstSCI CCNGG 6 cut(s) 85, 460, 526, 3330, 3801, 4879
BstSFI CTRYAG 4 cut(s) 2913, 3149, 3755, 4926
BstSLI GKGCMC 1 cut(s) 4715
BstSNI TACGTA 1 cut(s) 1680
BstV2I GAAGAC 2 cut(s) 363, 948
BstX2I RGATCY 8 cut(s) 1574, 3176, 3475, 3943, 3982, 4481, 4822, 4906
BstYI RGATCY 8 cut(s) 1574, 3176, 3475, 3943, 3982, 4481, 4822, 4906
Bsu15I ATCGAT 3 cut(s) 132, 3785, 4532
BsuI GTATCC 1 cut(s) 1085
BsuTUI ATCGAT 3 cut(s) 132, 3785, 4532
BtgI CCRYGG 4 cut(s) 975, 2034, 2391, 3073
BtrI CACGTC 1 cut(s) 3156
BtsI GCAGTG 2 cut(s) 42, 1476
BveI ACCTGC 2 cut(s) 4017, 4433
CaiI CAGNNNCTG 4 cut(s) 1187, 1850, 2774, 4925
CciI TCATGA 5 cut(s) 283, 2758, 2833, 4042, 4991
CfoI GCGC 3 cut(s) 2325, 4125, 4642
Cfr10I RCCGGY 2 cut(s) 361, 1734
Cfr13I GGNCC 6 cut(s) 458, 1822, 2410, 3067, 3293, 3799
ClaI ATCGAT 3 cut(s) 132, 3785, 4532
CseI GACGC 1 cut(s) 2119
Csp6I GTAC 7 cut(s) 255, 871, 1286, 1762, 2777, 3410, 3677
CspCI CAANNNNNGTGG 4 cut(s) 1492, 1527, 2051, 2086
CviQI GTAC 7 cut(s) 255, 871, 1286, 1762, 2777, 3410, 3677
DraI TTTAAA 1 cut(s) 2814
DraIII CACNNNGTG 1 cut(s) 3494
EaeI YGGCCR 2 cut(s) 359, 2634
Eam1104I CTCTTC 7 cut(s) 606, 1391, 2717, 3057, 3396, 3419, 4716
EarI CTCTTC 7 cut(s) 606, 1391, 2717, 3057, 3396, 3419, 4716
Ecl136II GAGCTC 1 cut(s) 2743
Eco105I TACGTA 1 cut(s) 1680
Eco130I CCWWGG 3 cut(s) 2034, 2391, 3073
Eco147I AGGCCT 1 cut(s) 2674
Eco24I GRGCYC 1 cut(s) 2745
Eco31I GGTCTC 1 cut(s) 1998
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 458, 2410
Eco53kI GAGCTC 1 cut(s) 2743
Eco57I CTGAAG 9 cut(s) 225, 557, 1644, 2307, 2535, 2574, 2751, 2894, 3420
Eco72I CACGTG 1 cut(s) 3491
Eco88I CYCGRG 1 cut(s) 2744
Eco91I GGTNACC 1 cut(s) 1730
EcoICRI GAGCTC 1 cut(s) 2743
EcoNI CCTNNNNNAGG 2 cut(s) 1694, 2346
EcoO109I RGGNCCY 1 cut(s) 3067
EcoO65I GGTNACC 1 cut(s) 1730
EcoRI GAATTC 2 cut(s) 1062, 2927
EcoRII CCWGG 4 cut(s) 460, 526, 3330, 4879
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 3 cut(s) 2034, 2391, 3073
EcoT22I ATGCAT 3 cut(s) 592, 3487, 4969
EcoT38I GRGCYC 1 cut(s) 2745
ErhI CCWWGG 3 cut(s) 2034, 2391, 3073
Esp3I CGTCTC 3 cut(s) 2137, 3398, 3554
FalI AAGNNNNNCTT 4 cut(s) 2433, 2465, 3870, 3902
FaqI GGGAC 3 cut(s) 125, 709, 3818
FauI CCCGC 2 cut(s) 2778, 4073
FauNDI CATATG 5 cut(s) 155, 1241, 2011, 4204, 4285
FbaI TGATCA 4 cut(s) 45, 280, 2704, 3214
FblI GTMKAC 1 cut(s) 2176
FriOI GRGCYC 1 cut(s) 2745
GlaI GCGC 3 cut(s) 2324, 4124, 4641
GsaI CCCAGC 1 cut(s) 31
GsuI CTGGAG 4 cut(s) 2784, 3085, 3191, 4351
HapII CCGG 6 cut(s) 87, 362, 1735, 1902, 3802, 4432
HgaI GACGC 1 cut(s) 2119
HhaI GCGC 3 cut(s) 2325, 4125, 4642
Hin6I GCGC 3 cut(s) 2323, 4123, 4640
HinP1I GCGC 3 cut(s) 2323, 4123, 4640
HincII GTYRAC 3 cut(s) 1367, 3048, 3744
HindII GTYRAC 3 cut(s) 1367, 3048, 3744
HindIII AAGCTT 3 cut(s) 347, 2328, 2906
HpaII CCGG 6 cut(s) 87, 362, 1735, 1902, 3802, 4432
Hpy166II GTNNAC 7 cut(s) 151, 1367, 2177, 2363, 3048, 3744, 4660
Hpy8I GTNNAC 7 cut(s) 151, 1367, 2177, 2363, 3048, 3744, 4660
Hpy99I CGWCG 2 cut(s) 95, 882
HspAI GCGC 3 cut(s) 2323, 4123, 4640
Kpn2I TCCGGA 1 cut(s) 4431
KpnI GGTACC 1 cut(s) 1765
Ksp22I TGATCA 4 cut(s) 45, 280, 2704, 3214
LguI GCTCTTC 1 cut(s) 1391
LmnI GCTCC 8 cut(s) 178, 858, 1247, 1528, 2803, 3101, 3505, 4612
MfeI CAATTG 1 cut(s) 2154
MflI RGATCY 8 cut(s) 1574, 3176, 3475, 3943, 3982, 4481, 4822, 4906
MhlI GDGCHC 3 cut(s) 1460, 2745, 4715
MlsI TGGCCA 1 cut(s) 2636
MluNI TGGCCA 1 cut(s) 2636
MlyI GAGTC 6 cut(s) 731, 749, 983, 1771, 2966, 3946
MmeI TCCRAC 5 cut(s) 77, 1799, 2979, 3219, 4374
Mox20I TGGCCA 1 cut(s) 2636
Mph1103I ATGCAT 3 cut(s) 592, 3487, 4969
MroI TCCGGA 1 cut(s) 4431
MroXI GAANNNNTTC 5 cut(s) 210, 1016, 2656, 3570, 4225
MscI TGGCCA 1 cut(s) 2636
MslI CAYNNNNRTG 6 cut(s) 1374, 1728, 2192, 2712, 4185, 4433
Msp20I TGGCCA 1 cut(s) 2636
MspA1I CMGCKG 5 cut(s) 1148, 1847, 2785, 4371, 4631
MspCI CTTAAG 3 cut(s) 2498, 4013, 4762
MspI CCGG 6 cut(s) 87, 362, 1735, 1902, 3802, 4432
MspR9I CCNGG 6 cut(s) 87, 462, 528, 3332, 3803, 4881
MunI CAATTG 1 cut(s) 2154
Mva1269I GAATGC 5 cut(s) 1543, 2132, 2846, 2894, 4084
MvaI CCWGG 4 cut(s) 462, 528, 3332, 4881
NciI CCSGG 2 cut(s) 87, 3803
NcoI CCATGG 3 cut(s) 2034, 2391, 3073
NdeI CATATG 5 cut(s) 155, 1241, 2011, 4204, 4285
NlaIV GGNNCC 7 cut(s) 1501, 1763, 3295, 3507, 3984, 4567, 4614
NmeAIII GCCGAG 2 cut(s) 2590, 4966
NsiI ATGCAT 3 cut(s) 592, 3487, 4969
NspI RCATGY 8 cut(s) 594, 1319, 1590, 3683, 3742, 4032, 4403, 4601
OliI CACNNNNGTG 1 cut(s) 2192
PaeI GCATGC 2 cut(s) 1319, 4032
PaeR7I CTCGAG 1 cut(s) 2744
PagI TCATGA 5 cut(s) 283, 2758, 2833, 4042, 4991
PaqCI CACCTGC 1 cut(s) 4433
PceI AGGCCT 1 cut(s) 2674
PciI ACATGT 2 cut(s) 1586, 3738
PciSI GCTCTTC 1 cut(s) 1391
PctI GAATGC 5 cut(s) 1543, 2132, 2846, 2894, 4084
PdmI GAANNNNTTC 5 cut(s) 210, 1016, 2656, 3570, 4225
PflMI CCANNNNNTGG 3 cut(s) 1022, 1831, 2807
PfoI TCCNGGA 1 cut(s) 3330
PleI GAGTC 6 cut(s) 730, 749, 983, 1771, 2965, 3945
PmaCI CACGTG 1 cut(s) 3491
PmlI CACGTG 1 cut(s) 3491
PpsI GAGTC 6 cut(s) 730, 749, 983, 1771, 2965, 3945
Ppu21I YACGTR 3 cut(s) 1680, 3491, 3719
PscI ACATGT 2 cut(s) 1586, 3738
PsiI TTATAA 1 cut(s) 2000
Psp124BI GAGCTC 1 cut(s) 2745
Psp1406I AACGTT 2 cut(s) 926, 2983
Psp6I CCWGG 4 cut(s) 460, 526, 3330, 4879
PspCI CACGTG 1 cut(s) 3491
PspEI GGTNACC 1 cut(s) 1730
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 4 cut(s) 460, 526, 3330, 4879
PspN4I GGNNCC 7 cut(s) 1501, 1763, 3295, 3507, 3984, 4567, 4614
PspPI GGNCC 6 cut(s) 458, 1822, 2410, 3067, 3293, 3799
PstI CTGCAG 3 cut(s) 2917, 3153, 4930
PstNI CAGNNNCTG 4 cut(s) 1187, 1850, 2774, 4925
PsuI RGATCY 8 cut(s) 1574, 3176, 3475, 3943, 3982, 4481, 4822, 4906
PvuII CAGCTG 3 cut(s) 1847, 4371, 4631
RsaI GTAC 7 cut(s) 256, 872, 1287, 1763, 2778, 3411, 3678
RsaNI GTAC 7 cut(s) 255, 871, 1286, 1762, 2777, 3410, 3677
RseI CAYNNNNRTG 6 cut(s) 1374, 1728, 2192, 2712, 4185, 4433
SacI GAGCTC 1 cut(s) 2745
SapI GCTCTTC 1 cut(s) 1391
Sau96I GGNCC 6 cut(s) 458, 1822, 2410, 3067, 3293, 3799
SchI GAGTC 6 cut(s) 731, 749, 983, 1771, 2966, 3946
ScrFI CCNGG 6 cut(s) 87, 462, 528, 3332, 3803, 4881
SduI GDGCHC 3 cut(s) 1460, 2745, 4715
SfcI CTRYAG 4 cut(s) 2913, 3149, 3755, 4926
Sfr274I CTCGAG 1 cut(s) 2744
SinI GGWCC 2 cut(s) 458, 2410
SlaI CTCGAG 1 cut(s) 2744
SmiMI CAYNNNNRTG 6 cut(s) 1374, 1728, 2192, 2712, 4185, 4433
SmlI CTYRAG 9 cut(s) 605, 1207, 2118, 2343, 2498, 2744, 3653, 4013, 4762
SmoI CTYRAG 9 cut(s) 605, 1207, 2118, 2343, 2498, 2744, 3653, 4013, 4762
SnaBI TACGTA 1 cut(s) 1680
SphI GCATGC 2 cut(s) 1319, 4032
SseBI AGGCCT 1 cut(s) 2674
SspI AATATT 3 cut(s) 1794, 2596, 4198
SstI GAGCTC 1 cut(s) 2745
StuI AGGCCT 1 cut(s) 2674
StyD4I CCNGG 6 cut(s) 85, 460, 526, 3330, 3801, 4879
StyI CCWWGG 3 cut(s) 2034, 2391, 3073
TatI WGTACW 3 cut(s) 254, 2776, 3676
TauI GCSGC 1 cut(s) 3395
TspGWI ACGGA 6 cut(s) 752, 833, 964, 1430, 3344, 3578
Van91I CCANNNNNTGG 3 cut(s) 1022, 1831, 2807
Vha464I CTTAAG 3 cut(s) 2498, 4013, 4762
VpaK11BI GGWCC 2 cut(s) 458, 2410
XagI CCTNNNNNAGG 2 cut(s) 1694, 2346
XbaI TCTAGA 3 cut(s) 3179, 3205, 4585
XceI RCATGY 8 cut(s) 594, 1319, 1590, 3683, 3742, 4032, 4403, 4601
XhoI CTCGAG 1 cut(s) 2744
XmiI GTMKAC 1 cut(s) 2176
XmnI GAANNNNTTC 5 cut(s) 210, 1016, 2656, 3570, 4225
Zsp2I ATGCAT 3 cut(s) 592, 3487, 4969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.