Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0003903

Physical Location & Seq

Genomic Coordinates Reverse (-)

61584121 .. 61594191

Gene Structure 10071 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G033520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5013 bp

ATGGGTAGAAGGAAGCAAAGCCGGCCTCACCGGTCAGGTGGGATTGTATTAGAGAGTCAAGCAACTGCTGGCAGGGGAGAATTGGATGAGGGGAGGCTTTTAAATGGAGAAACGCAAAAGAATGACATAGATGAAGTTGATAGACCGTATTTTGTGGAAGTTGATCGGTCTAATTGGGGATCAGATGAGCACCTTGATATTGCTGAAGTGGTTTTAACAGATTTGACCTTCAGAGAAGGGTTTTGCAGTGATGTTTTTAGTGGGGGTTTGAATTGCGATAGTTACTCCTTGAGGTTCCGGTTATCTAATGTGAAGGAGTTCATTGGTCGTATTAAGCTTGGTCATTGGCCTTTGTTGTCCTCTGCTGATATATCTTTGGAATTCGTTAAAACATGTTCAAGTGAGAATATGGACATGGAGAAGGACACATGCTCAGTAATCTTGTCAGGGAGTTTTGATGGGCCAGATGAAGGTATTTCTGGTCTGGTTCACTTGGCAAGTTTGAAGTTTATGACACTGAGGCCAGCTCTGTCAATTGGTTTTGTGAATGATACGTCAACCACCAGGGTCAGGGTGGAGATACTTAAGAATGCATTTGATGCTTGTGAGTCACTTCTGGACAATACTAGGCAAGTGTGGAAAAAGAGTATGATGAATGTCATGGCTTGGCTACACCCAGAAGTAGTGACTTCCGAGGCTAGGTATGGAGTCAGTAAATCAACAGACATGGAAATTGATTTGCATACTGACACTGGACATGCTAATTCAAATCATAGTCGACATGCAAGGTTTGATGTTGCTGGGTTCTATGAAGCCATTAAACCCTCAAAAGCAGATACAATGCTTCAAGATGTCCTGCCTGATTTGCTTCCTGAATTGAAACCATATCAACGCCGTGCAGCTTACTGGATGGTACAGAGGGAGAAAAGCGATGCACAAAGTCTGGCCCAAGAGGCCACAAGTCAGTTCATGTCGCCCTTATGTTTGCCCTTGCAATTTCTCGACAGATGTTCAAAAATGTTCTACAACCCATTCAGTGGAAATGTTTCGCTGCACCAAGAGCATTTTTCAACGTACGTCTCTGGTGGTATTCTTGCTGATGAGATGGGCATGGGGAAAACTGTTGAACTTCTTGCTTGCATCTTTGCTCATCAGAAGTCAGCAGATGAAGACCCCATATTTGCTGATACTGAAATTCAAGATACTGAGGATCTGAAAATAAATCTTAAAAGATTAAAAATGGAGCGAGTCGAATGTATATGTGGAGCTGTGAGTGAAAGTAGCAGATACAGAGGACTATGGGTACAGTGTGACATATGTGATGCATGGCAACATGCCAATTGTGTGGGCTATTCTCCTAGAGGAAAGACTACAAAATCCAACGAGGCTTCTAACGAAAGAGAACATGAAAAGAGTTTGGTGGTTAAGAAGTATACTAGAAAGAAGAACACAACCACAATAGATGTGAGAGATGAGGAATATATATGCCAGCTGTGCTCAGAACTGATACAGGCCACTAATTCTCCTGTTGCTACTGGTGCCACTCTTATAATCTGTCCAGCTTCTATAATGCCCCAGTGGTATTCTGAAATTATGCGTCATACGCGTTCAGGTTCTCTAAAGACTTGCGTCTATGAAGGTGTGAGAGAGGCGTCTTTTTCAAACACATCTGTAATTGATATCAGTGAACTCATCAGTGCTGACATTGTGTTGACAACATATGATGTGCTTAAAGCGGACCTGTCACATGACTCTGATAGGCATGAAGGTGATCGACGCTTAATGAGATTCCAAAAGAGATACCCTGTCGTTCCAACTACTCTAACCAGAATATTCTGGTGGAGAATTTGTTTGGATGAGGCTCAGATGGTGGAGAGTAATGCTGGTGCTGCAACAGAAATGGCTATGCGACTGTATGCGAAGCATCGTTGGTGCATAACGGGGACTCCTATACAACGAAAACTTGATGACTTATATGGACTTCTACGGTTTCTTAAAGCATGCCCTTTCAATGCTTCGAGGTGGTGGATTGAAGTTATACGAGATCCATATGAGAGGAGAGATGCAGGAGCTATGGAATTTACACATAAATTCTTTAAAGAAATCATGTGGCGATCTTCAAAAGTGCATGTTGCAGATGAGTTGCAGCTTCCACCTCAGGAAGAATGTCTCTCTTGGCTCACTTTATCTCCAGTTGAAGAGCATTTTTACCAGAGGCAACATGAAACTTGTGTGAGTTATGCCCGAGAAGTCATTGAAAGTTTGAAAGATGATATTATGAAGAGAAAAGTCGGAGGCTGCTCAGCAGCTAAATCATCAGATTCTTTCATCACACATGCGGAGGCAGGAAAGCTGCTGAACACACTTCTAAAGCTTCGCCAAGCCAGCTGTCACCCTCAAGTAGGAAGCTCAGGCCTGCGTTCTCTCCAACAGTCTCCCATGACTATGGAGGAAATATTGATGGTTCTTGTAAGTAAAACGAAGATAGAAGGAGAGGAAGCTCTCAGAAGGTTAGTTGTTGCTCTTAATGGGCTCGCTGGGATAGCTGTAATTGAGCAAAATTTTACTCAAGCTGTATCACTGTATAAGGAATCGTTGACTTTAGCTGAAGAGCACGCTGAGGATTTCCGCTTGGATCCTTTGTTGAGTATTCACATTCATCATAATCTTGCTGAAATACTCCCATTAGCAACAAGCTGCTCCCCTTCAAAAGCAGAACATATTCCTGGGACAATGGGCCCCTGTGACGAACATGTAGCGAAGAGGCGAAAACTGAGTGGGGAAGATAACTCATCAGAGAATGACTTTTCTATTGCCCAAGAATATAATAACGTGTCCCATGCATCGTGTTCATCCTTCACTCATGTAGCTTTGAGAACAATCTGTGGCAATCTTAAACAAAAGTACTTAGCTGCATTCAGTTCAAAGTTAATCATGACTCAGCAAGAGTTTAAGAAATCTTACACCCAGGTTTGTAGCACAATTAGTGAGATGAAAGATGTAGGTACAATTTGGTGGTTGGAAGCTCTTCTTCATGCTGAAAAGAATAGTGACATTTCTAGTCTCTTGATCAGAAAGATTGAAGAGGCGCTCATAGGAACTCTAAACAATTCAAAGTCATCTAAAATTCCATCTCGTTTACGAAGCATAAGCGCTCTAAAGTATCATATGCAGACCGGTTTGGACCAACTAGAAGAATCTAGAAAAATATTACTTGATCGACTTTTAGAAATTGATCAGACAATGGAGAAGCCGAAAGAGGAGGATATCCAGCGTGTGAGATACTGTCGAAATTGCAAAGCTAATGATGGTGGTCCCTTATGTGTTCTTTGTGAAGTAGATGAATTATTTCAGGGTTATGAAGCAAGGCTCTTTCGTCTTGAGAAAGTATCTGGAGGGATTGCTACCTCTGCTGAAGAGGCTGTGGATTTGCAGAAGAGGAACTCTGCACTTAATCGTTTCTATCAGAATTTGTCACAGCCGATTAAGGATTCAACATCACCCAAATCTAATCAAGAATCAAAGAAGAGAGATGTTGGAAAAGTTGTGGTTTCAAAATCCCCATCTGAATTAGAGGTTGTTCTTGGAGTTATAAAAAGCCATTGCAAGGCCCATTTAGGAAAGGAAGGTATATCAGAAGCTACCAAGCACCTACACATTCTGGAGGGCATGCGCAAGGAGTATGGCCATGCAAGATCCTTGGCAATAGCTCAAGCTCAGATTCTGCAAGCCTATGATGAAATTAATATGGCAACTACAAGGTTGCGCTTGAGTGAAGATGAGAATGATAAATCTCTTGATGCATTAAGTGAAGATGAATTACCTTCAGCTAATGTTTTATACACCAGTGAAAAGTTTGCATCCTTGAGCTTGTTGTCTTGTATAAAAGGGAAACTTCGTTATTTGAAGGGTTTGGTAGAAGCTAAACAAAAAATGCCATTTGGAAGTCCAAATCATTCCCCAGTAGGAGAGGAAGCCACCATATCAACTTCAACAGAACAGAGAAAAGAATGCATACCTACAGGTGATAAAGAAACGTGCCCAGTTTGTCAAGAACAATTAACCATTCGAAAGATGGTTTTCCCTTGTGGACATCTTACTTGCTGTAAATGTTTATTTGCAATTACTGAGAGACTACTCAATGGGAAAAAGGTTCAAGATAAGTGGGTAAAATGCCCAACATGTCGACAGCACACTGATGTTGCAAATATTGCTTTTGCTGATGATGGACATAGCGAATCTTCTGGTTCTGCTGGGCTTCATGCAATCCAAAGTCTTGAAGAGTGTGAAGCATCTATTATAGTTCAAGGTTCATATGGAACAAAGATTGAAGCAGTTACAAGAAGAATTCTGTGGATAAAGTCTACAGATCCAGAGGCTAAAGTTCTTGTTTTCTCAAGCTGGAATGATGTCCTTGACGTATTGGAGCATGCCTTCAGCGCTAATGGCATTACCTTTATAAGGATGAAAGGAGGAAGGAAATCACAAGTTGCCATTAGTGAATTTAAAGGAGAAAAGAGAAGCTTAACAGGAAATCACAAGTTGCATGGCCGAAACCCAGAAGGAAGATCTATTCAAGTGTTACTGCTCTTAATTCAACACGGAGCGAATGGCCTCAATCTGTTGGAAGCGAAACATGTTATTCTTATTGAGCCGCTTCTCAATCCAGCAGCAGAAGCACAAGCCATCAGCAGAGTACACCGAATCGGACAGAAAAATAAGACCCTCGCTCATCGTTTCATAGTTAAAGGCACAGTTGAAGAGAGCATATACAAGCTCAACAAAAGCAGGAACAGCACTTCCTTCATTAGTGGTAACACAAAGAACCAAGATCAGCCCTTATTGACACTAAAAGACATCGAGGCCTTATTTTCAACAGTACCATCATTAACTGTACCAGAGACTGATGAAAGGCCAACTGAAAGTGAAAGTTTAAGACATCTGCCACCTTCTAGGGCTGCTGCCATTGCAGCTGAGAAGCGGCTCAAGGAGCAGCATTCATGTTCGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0061630 GO:0061659 GO:0070013 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Helicase_C PHD SNF2_N zf-C3HC4

Protein Analysis

1670

Amino Acids

187.25

Weight (kDa)

6.48

Isoelectric Point (pI)

45.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNF2-rel_dom	PF00176	296 - 800	3.2e-63	SNF2-related domain
SHPRH_helical-1st	PF21325	816 - 898	1.6e-24	E3 ubiquitin-protein ligase SHPRH, first helical domain
SHPRH_helical-2nd	PF21324	1003 - 1127	1.6e-07	E3 ubiquitin-protein ligase SHPRH, second helical domain
Helicase_C	PF00271	1444 - 1570	5.3e-07	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003903)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40770 AT2G40770 AT2G40770
fragaria_vesca	FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110
malus_domestica	MD01G1169300.v1.1
prunus_persica	Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1
pyrus_communis	pycom01g18200
rosa_chinensis	RchiOBHm_Chr1g0372551
rosa_laevigata	RLG00000026892
rosa_multiflora	Rmu_sc0005410.1_g000019 Rmu_sc0011709.1_g000002
rosa_roxburghii	Rroxscaffold_4G00284860
rosa_rugosa	Rorug01G0372700
rosa_samantha	Rh1AG381200 Rh1BG344600 Rh1CG358000 Rh1DG375800
rosa_wichuraiana	Rw1G033520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1550, 3593, 4460
Acc16I	TGCGCA	1 cut(s)	3674
AccB1I	GGYRCC	1 cut(s)	1538
AccB7I	CCANNNNNTGG	1 cut(s)	1037
AccI	GTMKAC	4 cut(s)	778, 1433, 4186, 4364
AccII	CGCG	1 cut(s)	1606
AciI	CCGC	5 cut(s)	1736, 2339, 2629, 4655, 4981
AclWI	GGATC	7 cut(s)	187, 1218, 2039, 2630, 2643, 3690, 4364
AcoI	YGGCCR	2 cut(s)	3685, 4549
AcuI	CTGAAG	6 cut(s)	214, 225, 2628, 3435, 3810, 4420
AcyI	GRCGYC	1 cut(s)	1652
AfaI	GTAC	8 cut(s)	915, 1076, 1305, 2906, 3007, 4698, 4881, 4896
AfeI	AGCGCT	2 cut(s)	3154, 4441
AfiI	CCNNNNNNNGG	7 cut(s)	470, 570, 699, 1037, 2402, 3607, 4461
AflII	CTTAAG	1 cut(s)	584
AflIII	ACRYGT	6 cut(s)	392, 1604, 2752, 2832, 4181, 4636
AgeI	ACCGGT	2 cut(s)	30, 3176
AjnI	CCWGG	3 cut(s)	563, 2725, 2967
AloI	GAACNNNNNNTCC	2 cut(s)	4784, 4816
Alw21I	GWGCWC	3 cut(s)	192, 1499, 2616
Alw26I	GTCTC	6 cut(s)	1084, 2174, 2439, 3068, 4126, 4895
AlwI	GGATC	7 cut(s)	187, 1218, 2039, 2630, 2643, 3690, 4364
AlwNI	CAGNNNCTG	2 cut(s)	3724, 4904
Ama87I	CYCGRG	1 cut(s)	2244
Aor51HI	AGCGCT	2 cut(s)	3154, 4441
ApaI	GGGCCC	1 cut(s)	2741
ArsI	GACNNNNNNTTYG	2 cut(s)	1163, 1195
AseI	ATTAAT	1 cut(s)	3744
AsiGI	ACCGGT	2 cut(s)	30, 3176
Asp700I	GAANNNNTTC	5 cut(s)	317, 1045, 2721, 3027, 3348
AspLEI	GCGC	5 cut(s)	3091, 3155, 3675, 3768, 4442
AspS9I	GGNCC	8 cut(s)	461, 946, 1738, 2737, 2738, 3184, 3314, 3610
AsuHPI	GGTGA	5 cut(s)	20, 1781, 2384, 3492, 4037
AsuII	TTCGAA	1 cut(s)	4069
AvaI	CYCGRG	1 cut(s)	2244
AvaII	GGWCC	3 cut(s)	1738, 3184, 3314
AxyI	CCTNAGG	1 cut(s)	2157
BaeGI	GKGCMC	2 cut(s)	2741, 4043
BalI	TGGCCA	1 cut(s)	3687
BamHI	GGATCC	1 cut(s)	2635
BanI	GGYRCC	1 cut(s)	1538
BanII	GRGCYC	2 cut(s)	2535, 2741
BarI	GAAGNNNNNNTAC	2 cut(s)	3804, 3836
BbsI	GAAGAC	1 cut(s)	1176
Bbv12I	GWGCWC	3 cut(s)	192, 1499, 2616
BbvCI	CCTCAGC	1 cut(s)	2619
BceAI	ACGGC	1 cut(s)	879
BciT130I	CCWGG	3 cut(s)	565, 2727, 2969
BclI	TGATCA	2 cut(s)	3069, 3235
BcoDI	GTCTC	6 cut(s)	1084, 2174, 2439, 3068, 4126, 4895
BfaI	CTAG	8 cut(s)	627, 699, 1359, 1437, 3060, 3191, 3201, 4953
BfmI	CTRYAG	2 cut(s)	4020, 4365
BfoI	RGCGCY	3 cut(s)	3092, 3156, 4443
BfrI	CTTAAG	1 cut(s)	584
BglI	GCCNNNNNGGC	1 cut(s)	953
BglII	AGATCT	1 cut(s)	4568
BlpI	GCTNAGC	1 cut(s)	2302
BmcAI	AGTACT	1 cut(s)	2906
Bme1390I	CCNGG	3 cut(s)	565, 2727, 2969
Bme18I	GGWCC	3 cut(s)	1738, 3184, 3314
BmeT110I	CYCGRG	1 cut(s)	2244
BmgT120I	GGNCC	8 cut(s)	461, 946, 1738, 2737, 2738, 3184, 3314, 3610
BmiI	GGNNCC	6 cut(s)	296, 1540, 2637, 2739, 2740, 3316
BmrFI	CCNGG	3 cut(s)	565, 2727, 2969
BmrI	ACTGGG	3 cut(s)	1570, 3956, 4037
BmuI	ACTGGG	3 cut(s)	1570, 3956, 4037
BoxI	GACNNNNGTC	1 cut(s)	1628
BpiI	GAAGAC	1 cut(s)	1176
BplI	GAGNNNNNCTC	2 cut(s)	511, 543
BpmI	CTGGAG	3 cut(s)	2175, 3414, 3683
Bpu10I	CCTNAGC	2 cut(s)	2410, 2619
Bpu1102I	GCTNAGC	1 cut(s)	2302
Bpu14I	TTCGAA	1 cut(s)	4069
BpuEI	CTTGAG	9 cut(s)	310, 2382, 2553, 3401, 3696, 3790, 3886, 4381, 4970
BsaHI	GRCGYC	1 cut(s)	1652
BsaJI	CCNNGG	5 cut(s)	564, 693, 2726, 2967, 3699
BsaWI	WCCGGW	3 cut(s)	30, 297, 3176
BsaXI	ACNNNNNCTCC	8 cut(s)	1507, 1537, 1834, 1864, 1930, 1960, 2173, 2203
Bsc4I	CCNNNNNNNGG	7 cut(s)	470, 570, 699, 1037, 2402, 3607, 4461
Bse118I	RCCGGY	3 cut(s)	21, 30, 3176
Bse1I	ACTGG	8 cut(s)	757, 911, 1540, 1576, 2192, 3846, 3962, 4043
Bse21I	CCTNAGG	1 cut(s)	2157
Bse3DI	GCAATG	2 cut(s)	3601, 4965
BseBI	CCWGG	3 cut(s)	565, 2727, 2969
BseDI	CCNNGG	5 cut(s)	564, 693, 2726, 2967, 3699
BseGI	GGATG	6 cut(s)	91, 915, 1861, 2852, 3860, 4470
BseLI	CCNNNNNNNGG	7 cut(s)	470, 570, 699, 1037, 2402, 3607, 4461
BseMI	GCAATG	2 cut(s)	3601, 4965
BseNI	ACTGG	8 cut(s)	757, 911, 1540, 1576, 2192, 3846, 3962, 4043
BseRI	GAGGAG	2 cut(s)	2071, 3275
BseSI	GKGCMC	2 cut(s)	2741, 4043
BseYI	CCCAGC	3 cut(s)	800, 2537, 4253
BsgI	GTGCAG	3 cut(s)	918, 1037, 3432
Bsh1236I	CGCG	1 cut(s)	1606
BshNI	GGYRCC	1 cut(s)	1538
BshTI	ACCGGT	2 cut(s)	30, 3176
BsiHKAI	GWGCWC	3 cut(s)	192, 1499, 2616
BsiHKCI	CYCGRG	1 cut(s)	2244
BsiSI	CCGG	4 cut(s)	22, 31, 298, 3177
BsiWI	CGTACG	1 cut(s)	1074
BslFI	GGGAC	4 cut(s)	1957, 2743, 2821, 3300
BslI	CCNNNNNNNGG	7 cut(s)	470, 570, 699, 1037, 2402, 3607, 4461
BsmAI	GTCTC	6 cut(s)	1084, 2174, 2439, 3068, 4126, 4895
BsmBI	CGTCTC	1 cut(s)	1084
BsmFI	GGGAC	4 cut(s)	1957, 2743, 2821, 3300
BsmI	GAATGC	4 cut(s)	595, 2915, 4016, 4996
BsoBI	CYCGRG	1 cut(s)	2244
Bsp119I	TTCGAA	1 cut(s)	4069
Bsp120I	GGGCCC	1 cut(s)	2737
Bsp1286I	GDGCHC	6 cut(s)	192, 1499, 2535, 2616, 2741, 4043
Bsp1720I	GCTNAGC	1 cut(s)	2302
BspACI	CCGC	5 cut(s)	1736, 2339, 2629, 4655, 4981
BspFNI	CGCG	1 cut(s)	1606
BspHI	TCATGA	2 cut(s)	2934, 5009
BspLI	GGNNCC	6 cut(s)	296, 1540, 2637, 2739, 2740, 3316
BspPI	GGATC	7 cut(s)	187, 1218, 2039, 2630, 2643, 3690, 4364
BspQI	GCTCTTC	3 cut(s)	2193, 2604, 3033
BspT104I	TTCGAA	1 cut(s)	4069
BspT107I	GGYRCC	1 cut(s)	1538
BspTI	CTTAAG	1 cut(s)	584
BsrDI	GCAATG	2 cut(s)	3601, 4965
BsrFI	RCCGGY	3 cut(s)	21, 30, 3176
BsrI	ACTGG	8 cut(s)	757, 911, 1540, 1576, 2192, 3846, 3962, 4043
BssAI	RCCGGY	3 cut(s)	21, 30, 3176
BssECI	CCNNGG	5 cut(s)	564, 693, 2726, 2967, 3699
BssNAI	GTATAC	1 cut(s)	1434
BssNI	GRCGYC	1 cut(s)	1652
BssT1I	CCWWGG	1 cut(s)	3699
Bst1107I	GTATAC	1 cut(s)	1434
Bst2UI	CCWGG	3 cut(s)	565, 2727, 2969
BstACI	GRCGYC	1 cut(s)	1652
BstAFI	CTTAAG	1 cut(s)	584
BstAPI	GCANNNNNTGC	2 cut(s)	599, 4211
BstBI	TTCGAA	1 cut(s)	4069
BstENI	CCTNNNNNAGG	2 cut(s)	2400, 4459
BstF5I	GGATG	6 cut(s)	91, 915, 1861, 2852, 3860, 4470
BstFNI	CGCG	1 cut(s)	1606
BstH2I	RGCGCY	3 cut(s)	3092, 3156, 4443
BstHHI	GCGC	5 cut(s)	3091, 3155, 3675, 3768, 4442
BstMAI	GTCTC	6 cut(s)	1084, 2174, 2439, 3068, 4126, 4895
BstNI	CCWGG	3 cut(s)	565, 2727, 2969
BstPAI	GACNNNNGTC	1 cut(s)	1628
BstSCI	CCNGG	3 cut(s)	563, 2725, 2967
BstSFI	CTRYAG	2 cut(s)	4020, 4365
BstSLI	GKGCMC	2 cut(s)	2741, 4043
BstUI	CGCG	1 cut(s)	1606
BstV2I	GAAGAC	1 cut(s)	1176
BstX2I	RGATCY	6 cut(s)	1210, 2044, 2635, 3695, 4369, 4568
BstXI	CCANNNNNNTGG	2 cut(s)	1345, 2446
BstYI	RGATCY	6 cut(s)	1210, 2044, 2635, 3695, 4369, 4568
BstZ17I	GTATAC	1 cut(s)	1434
Bsu36I	CCTNAGG	1 cut(s)	2157
BtgZI	GCGATG	1 cut(s)	945
BtsCI	GGATG	6 cut(s)	91, 915, 1861, 2852, 3860, 4470
BtsI	GCAGTG	1 cut(s)	253
CaiI	CAGNNNCTG	2 cut(s)	3724, 4904
CciI	TCATGA	2 cut(s)	2934, 5009
CfoI	GCGC	5 cut(s)	3091, 3155, 3675, 3768, 4442
Cfr10I	RCCGGY	3 cut(s)	21, 30, 3176
Cfr13I	GGNCC	8 cut(s)	461, 946, 1738, 2737, 2738, 3184, 3314, 3610
CseI	GACGC	4 cut(s)	1586, 1618, 1641, 1785
Csp6I	GTAC	8 cut(s)	914, 1075, 1304, 2905, 3006, 4697, 4880, 4895
CspAI	ACCGGT	2 cut(s)	30, 3176
CspCI	CAANNNNNGTGG	2 cut(s)	4145, 4180
CviQI	GTAC	8 cut(s)	914, 1075, 1304, 2905, 3006, 4697, 4880, 4895
DraI	TTTAAA	3 cut(s)	102, 2098, 4507
EaeI	YGGCCR	2 cut(s)	3685, 4549
Eco130I	CCWWGG	1 cut(s)	3699
Eco147I	AGGCCT	2 cut(s)	2415, 4865
Eco24I	GRGCYC	2 cut(s)	2535, 2741
Eco32I	GATATC	2 cut(s)	1681, 3268
Eco47I	GGWCC	3 cut(s)	1738, 3184, 3314
Eco47III	AGCGCT	2 cut(s)	3154, 4441
Eco57I	CTGAAG	6 cut(s)	214, 225, 2628, 3435, 3810, 4420
Eco81I	CCTNAGG	1 cut(s)	2157
Eco88I	CYCGRG	1 cut(s)	2244
EcoNI	CCTNNNNNAGG	2 cut(s)	2400, 4459
EcoO109I	RGGNCCY	1 cut(s)	2738
EcoRI	GAATTC	2 cut(s)	380, 4347
EcoRII	CCWGG	3 cut(s)	563, 2725, 2967
EcoRV	GATATC	2 cut(s)	1681, 3268
EcoT14I	CCWWGG	1 cut(s)	3699
EcoT22I	ATGCAT	5 cut(s)	595, 1327, 2845, 3805, 4016
EcoT38I	GRGCYC	2 cut(s)	2535, 2741
ErhI	CCWWGG	1 cut(s)	3699
Esp3I	CGTCTC	1 cut(s)	1084
FalI	AAGNNNNNCTT	6 cut(s)	3015, 3047, 3879, 3911, 4508, 4540
FaqI	GGGAC	4 cut(s)	1957, 2743, 2821, 3300
FauNDI	CATATG	5 cut(s)	1316, 1720, 2050, 3168, 4315
FbaI	TGATCA	2 cut(s)	3069, 3235
FblI	GTMKAC	4 cut(s)	778, 1433, 4186, 4364
FokI	GGATG	6 cut(s)	98, 922, 1868, 2839, 3847, 4477
FriOI	GRGCYC	2 cut(s)	2535, 2741
FspBI	CTAG	8 cut(s)	627, 699, 1359, 1437, 3060, 3191, 3201, 4953
FspI	TGCGCA	1 cut(s)	3674
GlaI	GCGC	5 cut(s)	3090, 3154, 3674, 3767, 4441
GsaI	CCCAGC	3 cut(s)	804, 2541, 4257
GsuI	CTGGAG	3 cut(s)	2175, 3414, 3683
HaeII	RGCGCY	3 cut(s)	3092, 3156, 4443
HapII	CCGG	4 cut(s)	22, 31, 298, 3177
HgaI	GACGC	4 cut(s)	1586, 1618, 1641, 1785
HhaI	GCGC	5 cut(s)	3091, 3155, 3675, 3768, 4442
Hin1I	GRCGYC	1 cut(s)	1652
Hin6I	GCGC	5 cut(s)	3089, 3153, 3673, 3766, 4440
HinP1I	GCGC	5 cut(s)	3089, 3153, 3673, 3766, 4440
HincII	GTYRAC	5 cut(s)	558, 779, 1713, 2598, 4187
HindII	GTYRAC	5 cut(s)	558, 779, 1713, 2598, 4187
HindIII	AAGCTT	3 cut(s)	335, 2372, 4522
HpaII	CCGG	4 cut(s)	22, 31, 298, 3177
HphI	GGTGA	5 cut(s)	20, 1781, 2384, 3492, 4037
Hpy99I	CGWCG	1 cut(s)	1779
HpyCH4IV	ACGT	6 cut(s)	554, 1073, 1077, 2832, 4037, 4419
HpySE526I	ACGT	6 cut(s)	554, 1073, 1077, 2832, 4037, 4419
Hsp92I	GRCGYC	1 cut(s)	1652
HspAI	GCGC	5 cut(s)	3089, 3153, 3673, 3766, 4440
KroI	GCCGGC	1 cut(s)	21
KroNI	GCCGGC	1 cut(s)	23
Ksp22I	TGATCA	2 cut(s)	3069, 3235
LguI	GCTCTTC	3 cut(s)	2193, 2604, 3033
LmnI	GCTCC	7 cut(s)	1243, 1265, 2069, 2705, 4426, 4604, 4990
MaeI	CTAG	8 cut(s)	627, 699, 1359, 1437, 3060, 3191, 3201, 4953
MaeII	ACGT	6 cut(s)	554, 1073, 1077, 2832, 4037, 4419
MfeI	CAATTG	2 cut(s)	534, 1339
MflI	RGATCY	6 cut(s)	1210, 2044, 2635, 3695, 4369, 4568
MhlI	GDGCHC	6 cut(s)	192, 1499, 2535, 2616, 2741, 4043
MlsI	TGGCCA	1 cut(s)	3687
MluI	ACGCGT	1 cut(s)	1604
MluNI	TGGCCA	1 cut(s)	3687
MlyI	GAGTC	7 cut(s)	64, 617, 717, 1257, 1745, 1939, 2932
MmeI	TCCRAC	7 cut(s)	1404, 1838, 2272, 2452, 3000, 3517, 4605
Mox20I	TGGCCA	1 cut(s)	3687
Mph1103I	ATGCAT	5 cut(s)	595, 1327, 2845, 3805, 4016
MroNI	GCCGGC	1 cut(s)	21
MroXI	GAANNNNTTC	5 cut(s)	317, 1045, 2721, 3027, 3348
MscI	TGGCCA	1 cut(s)	3687
MslI	CAYNNNNRTG	3 cut(s)	1767, 2444, 4197
Msp20I	TGGCCA	1 cut(s)	3687
MspA1I	CMGCKG	3 cut(s)	1492, 2388, 4973
MspCI	CTTAAG	1 cut(s)	584
MspI	CCGG	4 cut(s)	22, 31, 298, 3177
MspR9I	CCNGG	3 cut(s)	565, 2727, 2969
MunI	CAATTG	2 cut(s)	534, 1339
Mva1269I	GAATGC	4 cut(s)	595, 2915, 4016, 4996
MvaI	CCWGG	3 cut(s)	565, 2727, 2969
MvnI	CGCG	1 cut(s)	1606
NaeI	GCCGGC	1 cut(s)	23
NdeI	CATATG	5 cut(s)	1316, 1720, 2050, 3168, 4315
NgoMIV	GCCGGC	1 cut(s)	21
NlaIV	GGNNCC	6 cut(s)	296, 1540, 2637, 2739, 2740, 3316
NmuCI	GTSAC	8 cut(s)	609, 685, 1310, 1743, 2390, 2744, 3050, 3474
NsbI	TGCGCA	1 cut(s)	3674
NsiI	ATGCAT	5 cut(s)	595, 1327, 2845, 3805, 4016
NspV	TTCGAA	1 cut(s)	4069
PaeI	GCATGC	3 cut(s)	2004, 3673, 4433
PagI	TCATGA	2 cut(s)	2934, 5009
PceI	AGGCCT	2 cut(s)	2415, 4865
PciI	ACATGT	4 cut(s)	392, 2752, 4181, 4636
PciSI	GCTCTTC	3 cut(s)	2193, 2604, 3033
PctI	GAATGC	4 cut(s)	595, 2915, 4016, 4996
PdiI	GCCGGC	1 cut(s)	23
PdmI	GAANNNNTTC	5 cut(s)	317, 1045, 2721, 3027, 3348
PfeI	GAWTC	9 cut(s)	1788, 2321, 2591, 3197, 3491, 3518, 3721, 4238, 4704
Pfl23II	CGTACG	1 cut(s)	1074
PflMI	CCANNNNNTGG	1 cut(s)	1037
PinAI	ACCGGT	2 cut(s)	30, 3176
PleI	GAGTC	7 cut(s)	63, 616, 716, 1256, 1745, 1939, 2932
PpsI	GAGTC	7 cut(s)	63, 616, 716, 1256, 1745, 1939, 2932
PscI	ACATGT	4 cut(s)	392, 2752, 4181, 4636
PshAI	GACNNNNGTC	1 cut(s)	1628
PshBI	ATTAAT	1 cut(s)	3744
PsiI	TTATAA	3 cut(s)	1550, 3593, 4460
Psp6I	CCWGG	3 cut(s)	563, 2725, 2967
PspFI	CCCAGC	3 cut(s)	800, 2537, 4253
PspGI	CCWGG	3 cut(s)	563, 2725, 2967
PspLI	CGTACG	1 cut(s)	1074
PspN4I	GGNNCC	6 cut(s)	296, 1540, 2637, 2739, 2740, 3316
PspOMI	GGGCCC	1 cut(s)	2737
PspPI	GGNCC	8 cut(s)	461, 946, 1738, 2737, 2738, 3184, 3314, 3610
PstNI	CAGNNNCTG	2 cut(s)	3724, 4904
PsuI	RGATCY	6 cut(s)	1210, 2044, 2635, 3695, 4369, 4568
PvuII	CAGCTG	3 cut(s)	1492, 2388, 4973
RsaI	GTAC	8 cut(s)	915, 1076, 1305, 2906, 3007, 4698, 4881, 4896
RsaNI	GTAC	8 cut(s)	914, 1075, 1304, 2905, 3006, 4697, 4880, 4895
RseI	CAYNNNNRTG	3 cut(s)	1767, 2444, 4197
SalI	GTCGAC	2 cut(s)	777, 4185
SapI	GCTCTTC	3 cut(s)	2193, 2604, 3033
Sau96I	GGNCC	8 cut(s)	461, 946, 1738, 2737, 2738, 3184, 3314, 3610
ScaI	AGTACT	1 cut(s)	2906
SchI	GAGTC	7 cut(s)	64, 617, 717, 1257, 1745, 1939, 2932
ScrFI	CCNGG	3 cut(s)	565, 2727, 2969
SduI	GDGCHC	6 cut(s)	192, 1499, 2535, 2616, 2741, 4043
SfcI	CTRYAG	2 cut(s)	4020, 4365
SfiI	GGCCNNNNNGGCC	1 cut(s)	953
SfuI	TTCGAA	1 cut(s)	4069
SinI	GGWCC	3 cut(s)	1738, 3184, 3314
SmiMI	CAYNNNNRTG	3 cut(s)	1767, 2444, 4197
SphI	GCATGC	3 cut(s)	2004, 3673, 4433
SseBI	AGGCCT	2 cut(s)	2415, 4865
SsiI	CCGC	5 cut(s)	1736, 2339, 2629, 4655, 4981
SspI	AATATT	4 cut(s)	1833, 2457, 3210, 4210
SspMI	CTAG	8 cut(s)	627, 699, 1359, 1437, 3060, 3191, 3201, 4953
StuI	AGGCCT	2 cut(s)	2415, 4865
StyD4I	CCNGG	3 cut(s)	563, 2725, 2967
StyI	CCWWGG	1 cut(s)	3699
TaiI	ACGT	6 cut(s)	557, 1076, 1080, 2835, 4040, 4422
TaqII	GACCGA	1 cut(s)	156
TatI	WGTACW	2 cut(s)	2904, 4696
TauI	GCSGC	2 cut(s)	4657, 4984
TfiI	GAWTC	9 cut(s)	1788, 2321, 2591, 3197, 3491, 3518, 3721, 4238, 4704
TseFI	GTSAC	8 cut(s)	609, 685, 1310, 1743, 2390, 2744, 3050, 3474
Tsp45I	GTSAC	8 cut(s)	609, 685, 1310, 1743, 2390, 2744, 3050, 3474
TspGWI	ACGGA	1 cut(s)	4617
Van91I	CCANNNNNTGG	1 cut(s)	1037
Vha464I	CTTAAG	1 cut(s)	584
VpaK11BI	GGWCC	3 cut(s)	1738, 3184, 3314
VspI	ATTAAT	1 cut(s)	3744
XagI	CCTNNNNNAGG	2 cut(s)	2400, 4459
XbaI	TCTAGA	1 cut(s)	3200
XcmI	CCANNNNNNNNNTGG	2 cut(s)	571, 4072
XmiI	GTMKAC	4 cut(s)	778, 1433, 4186, 4364
XmnI	GAANNNNTTC	5 cut(s)	317, 1045, 2721, 3027, 3348
XspI	CTAG	8 cut(s)	627, 699, 1359, 1437, 3060, 3191, 3201, 4953
ZrmI	AGTACT	1 cut(s)	2906
Zsp2I	ATGCAT	5 cut(s)	595, 1327, 2845, 3805, 4016

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.