RchiOBHm_Chr1g0372551

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003903
Physical Location & Seq
Reverse (-)
61202203 .. 61213045
10843 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59652

Sequence Viewer

Length: 5013 bp
ATGGGTAGAAGGAAGCAAAGCCGGCCTCACCGGTCAGGTGGGATTGTATTAGAGAGTCAAGCAACTGCTGGCAGGGGAGAATTGGATGAGGGGAGGCTTTTAAATGGAGAAACGCAAAAGAATGACATAGATGAAGTTGATAGACCGTATTTTGTGGAAGTTGATCGGTCTAATTGGGGATCAGATGAGCACCTTGATATTGCTGAAGTGGTTTTAACAGATTTGACCTTCAGAGAAGGGTTTTGCAGTGACGTTTTTAGTGGGGGTTTGAATTGCGATAGTTACTCCTTAAGGTTCCGGTTATCTAATGTAAAGGAGTTCATTGGTCGTATTAAGCTTGGTCATTGGCCTTTGTTGTCCTCTGCTGATATATCTTTGGAATTCGTTAAAACATGTTCAAGCGAGTATATGGACATGGACAAGGACACCTGCTCAGTAATCTTGTCAGGGAGTTTTGATGGACCAGATGAAGGTATTTCTGGTCTGGTACACTTGGCAAGTTTGAAGTTTATGACACTGAGGCCAGCTCTGTCCATTGGGTTTGTGAATGATACGTCAACCATCAGGGTCAGGGTGGAGATACTTAAGAGTGCATTTAATGCTTGTGAGTCACTTCTTGACAATACTAGGCAAGTGTGGAAAAAGAGTATGATGAATGTCATGGCTTGGCTACACCCAGAAGTAGTGACTTCCGAGGCTAGGTATGGAGTCAGTAGATCAACAGACATGGAGATTGATTCGCATACCGACACTGGACATGCTAATTCCAATCATAGTAAACATGCAAGGTTTGATGTTGCTGGGTTCTATGAAGCCATTAAACCCTCAAAAGCAGATACAATGCTTCAAGATGTCCTGCCTGATTTGCTTCCTGAATTGAAACCATATCAACGCCGTGCAGCTTACTGGATGGTACAGAGGGAGAAAAGCGATGCACAAAGTCTGGCCCAAGAGGCCACAAGTCAGTTCATGTCGCCCTTATGTTTGCCCTTGCAATTTCTCGACAGATGTTCAAAAATGTTCTACAACCCATTCAGTGGAAATGTTTCGCTGCACCAAGAGCATTTTTCAACGTACGTCTCTGGCGGTATTCTTGCTGATGAGATGGGCATGGGGAAAACTGTTGAACTTCTCGCCTGCATCTTTGCTCATCAGAAGTCAGCAGATGAAGACCCCATATTTGCTGATGCTGAAATTCAAGATACTGAGGATCTGAAAATAAAGCTTAAAAGATTAAAAAGGGAGCGAGTTGAATGTATATGTGGAGCTGTGAGTGAAAGTATCAGATACAGAGGACTATGGGTACAGTGCGACATATGTGATGCATGGCAACATGCTGATTGTGTGGGCTATTCACCTAGAGGAAAGACTATAAAATCCAACGAGACTTCTAACGAAAAAGAACATGATAAGAGTTCGGTGGTTAAGAATTATACTAGAAAGAAGAATACAGCCACAATAGATGTGAGAGATGAGGAATATATATGCCAGCTGTGCTCAGAACTGATACAGGCCACTACTTCTCCTGTTGCTACTGGTGCCACTCTTATAATCTGTCCAGCTTCTATATTGCCCCAGTGGTATTCTGAAATTATGCGCCATACGCGTTCAGGTTCTTTAAAGACTTGCGTCTATGAAGGTGTAAGAGAGGCATCTTTTTCAAACACATCTGTAATTGATATCAGTGAACTCATCAGTGCTGACATTGTGTTGACAACATATGATGTGCTTAAAGCGGACCTGTCACATGACTCTGATAGGCATGAAGGTGATCGACGCTTAATGAGATTCCAAAAGAGATACCCTGTTGTTCCAACTATTCTTACCAGAATATTCTGGTGGAGAATTTGTTTGGATGAGGCTCAGATGGTGGAGAGTAATGCTGGTGCTGCAACAGAAATGGCTATGCGACTGTATGCGAAGCATCGTTGGTGCATAACGGGGACTCCTATACAACGTAAACTTGATGACTTGTATGGACTTCTACGGTTTCTTAAAGCATGCCCTTTCAATGCTTCCAGGTGGTGGATTGAAGTTATACGAGATCCATATGAGAGGAGAGATGCAGGAGCTATGGAATTTACACATAAATTCTTTAAAGAAATCATGTGGCGATCTTCAAAGGTGCATGTTGCAGATGAGTTGCAGCTTCCACCTCAGGAAGAATGTCTCTCTTGGCTCACTTTATCTCCAGTTGAAGAGCATTTTTACCAGAGGCAACATGAAACTTGTGTGAGTTATGCCCGAGAAGTCATTGAACATATGAAAGATGATATTATGAACAGAAAAGTCAGAGGATGCTCAGCAGCTAAATCATCAGATTCTTTCATCACACATGCGGAGGCAGGAAAGCTGCTGAACACACTTCTGAAGCTTCGCCAAGCCAGCTGTCACCCTCAAGTAGGAAGCTCAGGACTGCGTTCTCTCCAACAGTCTCCCATGACTATGGAGGAAATATTAATGGTTCTTGTAAGTAAAACCAAGATAGAAGGAGAGGAAGCTCTCAGAAGGTTAGTTGTTGCTCTTAATGGGCTCGCTGGGATAGCTGTAATTGAGCAAAATTTTACTCAAGCTGTATCACTGTATAAGGAATCGTTGACTTTAGCTGAAGAGCACACTGAGGATTTCCGCCTGGATCCTTTGTTGAATATTCACATTCATCATAATCTTGCCGAAATACTTCCATTAGCAACAAGCTGCTCCCCTTCAAAAGGACAACATATTCCTGGAAAAATGGGCCCCTGTGATGAACATGTAGCAAAGAGGCGAAAACTGGGTGGGGAAGATAACTCATCAGAGAATGACTTATCTAATGCTCAAGAATATAATAACTCCCATGCATCATGTAGTTCCTTCACTGACGTATCTTTGAGAACAGTCTGTGACAATCTTAAACAAAAGTACTTATCTGCATTCAGTTCAAAGTTATTCATGACTCAGCAAGAGTTTAAGAAGTCTTACACCCAGGTTTGTAGCACAATTAGTGAGATCAAAGATGTAAGTACAGTCTGGTGGCTGGAAGCCCTTCTTCATGCTGAAAAGAATAATGACATTTCTAGTCTGTTGATCAGAAAGATTGAAGAGGCGCTCATAGGAACTCTAAACAATTCAAAGTCATCTAGAATTCCATCTCGTTTACGAAGCATAAGCGCTCTAAAGTATCACATGCAGACCGGTTTGGACCAACTAGAAGAATCTAGGAAACTGTTACTTGATCGACTTTTAGAAATTGATCAGACAATGGAAAAGCCAAAAGAGGAGGATATCCAGCGTGTGAGATACTGTCGAAATTGCAAAGCTAATGATGGTGGTCCCTTATGTGTTCTTTGTGAAGTAGATGAATTATTTCGGGGATATGAAGCAAGGCTCTTTCGTCTTGAGAAAATATCTGGAGGGATTGCTACCTCTGCTGAAGAGGCTGTGGATTTGCAGAAGAAGAACTCTGAACTTAATCGTTTCTATCAGAATTTATCACAGCCAATTAAGGATTCAACGTCACCCAAATCTAATCAAGAATCGAAGAAGAGAGATGTTGGAAAAGTTGTGGTTTCAAAATCCCCATCTGAATTAGAGGTTGTTCTTGGAGTTATAAAAAGCCATTGCAAGGCCCATTTAGGAAAGGAAGGTATATCAGAAGCTACCAAGCACCTACACATTCTTGAGGGCATGCGCAAGGAGTATGGCCATGCAAGATCCTTGGCAATAGCTCAAGCTCAGATTCTGCAAGCTTATGATGAAATTAATATGGCAACTACAAGGTTGCGCCTGAGTGAAGATGAGAATGATAAATCTCTTGATGTATTAAGTGAAGATGAATTACCTTCAGCTAACGTTTTATACACCAGTGAAAAGTTCGCATCCTTGAGCTTGTTGTCTTGTATAAAAGGGAAGCTTCGTTATTTGAAGGGTTTGGTAGAAGCTAAACAAAAAATGCCATTTGGATGTCCAAATCATTCCTCAGTAACTGGAGAGGAAGCCACCATATCGACTTCTACAGAACAGAGAAATGAATGTGTACCTACAGGTGATAAAGAAACGTGCCCAGTTTGTCAAGAACAGTTAACCATTCAAAAGATGGTTTTCCCTTGTGGACATCTTACTTGCTGTAAATGTTTATTTGCAATTACTGAGAGACTACTAAATGGGAAAAAGGTTCAAGATAAGTGGGTAAAATGCCCAACATGTCGACAGCACACTGATGTTGCAAATATTGCTTTTGCTGATGATGGACAAAGTGAATCTTGTGGTTCTACTGGGCTTCATGCAATCCAAAGTCGTGAAGAGTGTGAAGCATCTATTATAGTTCAAGGTTCATATGGAACAAAGATTGAAGCAGTTACAAGAAGAATTCTGTGGATAAAGTCTACCGATCCAGAGGCTAAAGTTCTTGTTTTCTCAAGCTGGAATGATGTCCTTGACGTATTGGAGCATGCCTTCATTGCTAATGGCATTACCTCTATAAGGATGAAAGGAGGAAGGAAATCACAAGTTGCCATTAGTGAATTTAAAGGAGAAAAGAGAAGCTTAACAGGAAATCACAAGTTGCATGGCCGAAACCCAGAAGGAAGATCTATTCAAGTGTTGCTGCTCTTAATTCAACATGGAGCGAATGGCCTCAATCTGTTGGAAGCGAAGCATGTTATTCTTGTTGAGCCGCTTCTCAATCCAGCAGCAGAAGCACAAGCCATCGGCAGGGTACACCGTATCGGACAGAAAAATAAGACCCTCGCTCATCGTTTCATAGTTAAAGGCACGGTTGAAGAAAGCATATACAAGCTCAACAAAAGCAGGAACAGCACTGCCTTCATTAGTGGTAACACAAAAAACCAAGATCAGCCCTTATTGACACTGAAAGACATTGAGGCCTTATTTTCAACAGTGCCATCATTACCTGTACCAGAGACTGATGTAAGGCCAACTGAAAGTGAAACTCTAAGACATTTGCCACCTTCTGTGGCTGCTGCCATTGCAGCTGAGAAGCGGCTCAAGGAGCAGCATTCATGTTCTTCATGA

Protein Analysis

1670

Amino Acids

187.45

Weight (kDa)

6.47

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 296 - 800 2.4e-63 SNF2-related domain
SHPRH_helical-1st PF21325 816 - 895 2.4e-24 E3 ubiquitin-protein ligase SHPRH, first helical domain
SHPRH_helical-2nd PF21324 1002 - 1126 1.2e-06 E3 ubiquitin-protein ligase SHPRH, second helical domain
Helicase_C PF00271 1444 - 1570 1.2e-07 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1550, 3590
AarI CACCTGC 1 cut(s) 437
Acc16I TGCGCA 1 cut(s) 3671
Acc36I ACCTGC 1 cut(s) 437
AccB1I GGYRCC 1 cut(s) 1538
AccB7I CCANNNNNTGG 2 cut(s) 1037, 2025
AccI GTMKAC 2 cut(s) 4186, 4364
AccII CGCG 1 cut(s) 1606
AciI CCGC 6 cut(s) 1086, 1736, 2339, 2629, 4655, 4981
AclI AACGTT 1 cut(s) 3831
AclWI GGATC 7 cut(s) 187, 1218, 2039, 2630, 2643, 3687, 4364
AcoI YGGCCR 2 cut(s) 3682, 4549
AcuI CTGAAG 6 cut(s) 214, 225, 2390, 2628, 3432, 3807
AfaI GTAC 9 cut(s) 489, 915, 1076, 1305, 2903, 3004, 4017, 4698, 4896
AfeI AGCGCT 1 cut(s) 3151
AfiI CCNNNNNNNGG 9 cut(s) 470, 699, 1037, 2025, 2402, 2711, 3604, 4461, 4692
AflII CTTAAG 2 cut(s) 289, 584
AflIII ACRYGT 4 cut(s) 392, 1604, 2752, 4181
AgeI ACCGGT 2 cut(s) 30, 3173
AjnI CCWGG 4 cut(s) 2018, 2631, 2725, 2964
AjuI GAANNNNNNNTTGG 2 cut(s) 3472, 3504
Alw21I GWGCWC 3 cut(s) 192, 1499, 2616
Alw26I GTCTC 6 cut(s) 1084, 1379, 2174, 2439, 4126, 4895
AlwI GGATC 7 cut(s) 187, 1218, 2039, 2630, 2643, 3687, 4364
AlwNI CAGNNNCTG 3 cut(s) 3721, 3965, 4904
Ama87I CYCGRG 1 cut(s) 2244
Aor51HI AGCGCT 1 cut(s) 3151
ApaI GGGCCC 1 cut(s) 2741
ArsI GACNNNNNNTTYG 2 cut(s) 1163, 1195
AseI ATTAAT 2 cut(s) 2459, 3741
AsiGI ACCGGT 2 cut(s) 30, 3173
Asp700I GAANNNNTTC 4 cut(s) 1045, 2679, 3024, 3345
AspLEI GCGC 5 cut(s) 1599, 3088, 3152, 3672, 3765
AspS9I GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3181, 3311, 3607
AsuHPI GGTGA 6 cut(s) 20, 1347, 1781, 2384, 3489, 4037
AvaI CYCGRG 1 cut(s) 2244
AvaII GGWCC 4 cut(s) 461, 1738, 3181, 3311
AxyI CCTNAGG 1 cut(s) 2157
BaeGI GKGCMC 2 cut(s) 2741, 4043
BaeI ACNNNNGTAYC 2 cut(s) 2847, 2880
BalI TGGCCA 1 cut(s) 3684
BamHI GGATCC 1 cut(s) 2635
BanI GGYRCC 1 cut(s) 1538
BanII GRGCYC 2 cut(s) 2535, 2741
BarI GAAGNNNNNNTAC 2 cut(s) 3801, 3833
BbsI GAAGAC 1 cut(s) 1176
Bbv12I GWGCWC 3 cut(s) 192, 1499, 2616
BceAI ACGGC 1 cut(s) 879
BciT130I CCWGG 4 cut(s) 2020, 2633, 2727, 2966
BclI TGATCA 2 cut(s) 3066, 3232
BcoDI GTCTC 6 cut(s) 1084, 1379, 2174, 2439, 4126, 4895
BfaI CTAG 8 cut(s) 627, 699, 1359, 1437, 3057, 3120, 3188, 3198
BfmI CTRYAG 2 cut(s) 3993, 4020
BfoI RGCGCY 2 cut(s) 3089, 3153
BfrI CTTAAG 2 cut(s) 289, 584
BfuAI ACCTGC 1 cut(s) 437
BglI GCCNNNNNGGC 1 cut(s) 953
BglII AGATCT 1 cut(s) 4568
BlpI GCTNAGC 1 cut(s) 2302
BmcAI AGTACT 1 cut(s) 2903
Bme1390I CCNGG 4 cut(s) 2020, 2633, 2727, 2966
Bme18I GGWCC 4 cut(s) 461, 1738, 3181, 3311
BmeT110I CYCGRG 1 cut(s) 2244
BmgT120I GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3181, 3311, 3607
BmiI GGNNCC 6 cut(s) 296, 1540, 2637, 2739, 2740, 3313
BmrFI CCNGG 4 cut(s) 2020, 2633, 2727, 2966
BmrI ACTGGG 4 cut(s) 1570, 2783, 4037, 4263
BmuI ACTGGG 4 cut(s) 1570, 2783, 4037, 4263
BoxI GACNNNNGTC 1 cut(s) 1628
BpiI GAAGAC 1 cut(s) 1176
BplI GAGNNNNNCTC 2 cut(s) 511, 543
BpmI CTGGAG 3 cut(s) 2175, 3411, 3987
Bpu10I CCTNAGC 1 cut(s) 2410
Bpu1102I GCTNAGC 1 cut(s) 2302
BpuEI CTTGAG 9 cut(s) 2382, 2553, 2802, 3398, 3680, 3693, 3883, 4381, 4970
BsaJI CCNNGG 3 cut(s) 693, 2964, 3696
BsaWI WCCGGW 3 cut(s) 30, 297, 3173
BsaXI ACNNNNNCTCC 8 cut(s) 1507, 1537, 1834, 1864, 1930, 1960, 2173, 2203
Bsc4I CCNNNNNNNGG 9 cut(s) 470, 699, 1037, 2025, 2402, 2711, 3604, 4461, 4692
Bse118I RCCGGY 3 cut(s) 21, 30, 3173
Bse21I CCTNAGG 1 cut(s) 2157
Bse3DI GCAATG 3 cut(s) 3598, 4437, 4965
BseBI CCWGG 4 cut(s) 2020, 2633, 2727, 2966
BseDI CCNNGG 3 cut(s) 693, 2964, 3696
BseGI GGATG 7 cut(s) 91, 915, 1861, 2303, 3857, 3947, 4470
BseLI CCNNNNNNNGG 9 cut(s) 470, 699, 1037, 2025, 2402, 2711, 3604, 4461, 4692
BseMI GCAATG 3 cut(s) 3598, 4437, 4965
BseRI GAGGAG 2 cut(s) 2071, 3272
BseSI GKGCMC 2 cut(s) 2741, 4043
BseYI CCCAGC 2 cut(s) 800, 2537
BsgI GTGCAG 2 cut(s) 918, 1037
Bsh1236I CGCG 1 cut(s) 1606
BshNI GGYRCC 1 cut(s) 1538
BshTI ACCGGT 2 cut(s) 30, 3173
BsiHKAI GWGCWC 3 cut(s) 192, 1499, 2616
BsiHKCI CYCGRG 1 cut(s) 2244
BsiSI CCGG 4 cut(s) 22, 31, 298, 3174
BsiWI CGTACG 1 cut(s) 1074
BslFI GGGAC 2 cut(s) 1957, 3297
BslI CCNNNNNNNGG 9 cut(s) 470, 699, 1037, 2025, 2402, 2711, 3604, 4461, 4692
BsmAI GTCTC 6 cut(s) 1084, 1379, 2174, 2439, 4126, 4895
BsmBI CGTCTC 1 cut(s) 1084
BsmFI GGGAC 2 cut(s) 1957, 3297
BsmI GAATGC 2 cut(s) 2912, 4996
BsoBI CYCGRG 1 cut(s) 2244
Bsp120I GGGCCC 1 cut(s) 2737
Bsp1286I GDGCHC 6 cut(s) 192, 1499, 2535, 2616, 2741, 4043
Bsp1720I GCTNAGC 1 cut(s) 2302
BspACI CCGC 6 cut(s) 1086, 1736, 2339, 2629, 4655, 4981
BspFNI CGCG 1 cut(s) 1606
BspHI TCATGA 2 cut(s) 2931, 5009
BspLI GGNNCC 6 cut(s) 296, 1540, 2637, 2739, 2740, 3313
BspMI ACCTGC 1 cut(s) 437
BspPI GGATC 7 cut(s) 187, 1218, 2039, 2630, 2643, 3687, 4364
BspQI GCTCTTC 2 cut(s) 2193, 2604
BspT107I GGYRCC 1 cut(s) 1538
BspTI CTTAAG 2 cut(s) 289, 584
BsrDI GCAATG 3 cut(s) 3598, 4437, 4965
BsrFI RCCGGY 3 cut(s) 21, 30, 3173
BssAI RCCGGY 3 cut(s) 21, 30, 3173
BssECI CCNNGG 3 cut(s) 693, 2964, 3696
BssT1I CCWWGG 1 cut(s) 3696
Bst2UI CCWGG 4 cut(s) 2020, 2633, 2727, 2966
Bst6I CTCTTC 6 cut(s) 2193, 2604, 3075, 3408, 3518, 4275
BstAFI CTTAAG 2 cut(s) 289, 584
BstAPI GCANNNNNTGC 2 cut(s) 599, 4211
BstENI CCTNNNNNAGG 3 cut(s) 2400, 2709, 4459
BstF5I GGATG 7 cut(s) 91, 915, 1861, 2303, 3857, 3947, 4470
BstFNI CGCG 1 cut(s) 1606
BstH2I RGCGCY 2 cut(s) 3089, 3153
BstHHI GCGC 5 cut(s) 1599, 3088, 3152, 3672, 3765
BstMAI GTCTC 6 cut(s) 1084, 1379, 2174, 2439, 4126, 4895
BstNI CCWGG 4 cut(s) 2020, 2633, 2727, 2966
BstPAI GACNNNNGTC 1 cut(s) 1628
BstSCI CCNGG 4 cut(s) 2018, 2631, 2725, 2964
BstSFI CTRYAG 2 cut(s) 3993, 4020
BstSLI GKGCMC 2 cut(s) 2741, 4043
BstUI CGCG 1 cut(s) 1606
BstV2I GAAGAC 1 cut(s) 1176
BstX2I RGATCY 5 cut(s) 1210, 2044, 2635, 3692, 4568
BstXI CCANNNNNNTGG 1 cut(s) 2446
BstYI RGATCY 5 cut(s) 1210, 2044, 2635, 3692, 4568
Bsu36I CCTNAGG 1 cut(s) 2157
BtgZI GCGATG 1 cut(s) 945
BtsCI GGATG 7 cut(s) 91, 915, 1861, 2303, 3857, 3947, 4470
BtsI GCAGTG 2 cut(s) 253, 4797
BveI ACCTGC 1 cut(s) 437
CaiI CAGNNNCTG 3 cut(s) 3721, 3965, 4904
CciI TCATGA 2 cut(s) 2931, 5009
CfoI GCGC 5 cut(s) 1599, 3088, 3152, 3672, 3765
Cfr10I RCCGGY 3 cut(s) 21, 30, 3173
Cfr13I GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3181, 3311, 3607
CseI GACGC 2 cut(s) 1618, 1785
Csp6I GTAC 9 cut(s) 488, 914, 1075, 1304, 2902, 3003, 4016, 4697, 4895
CspAI ACCGGT 2 cut(s) 30, 3173
CspCI CAANNNNNGTGG 2 cut(s) 4145, 4180
CviQI GTAC 9 cut(s) 488, 914, 1075, 1304, 2902, 3003, 4016, 4697, 4895
DraI TTTAAA 4 cut(s) 102, 1620, 2098, 4507
EaeI YGGCCR 2 cut(s) 3682, 4549
Eam1104I CTCTTC 6 cut(s) 2193, 2604, 3075, 3408, 3518, 4275
EarI CTCTTC 6 cut(s) 2193, 2604, 3075, 3408, 3518, 4275
EciI GGCGGA 1 cut(s) 2618
Eco130I CCWWGG 1 cut(s) 3696
Eco147I AGGCCT 1 cut(s) 4865
Eco24I GRGCYC 2 cut(s) 2535, 2741
Eco32I GATATC 2 cut(s) 1681, 3265
Eco47I GGWCC 4 cut(s) 461, 1738, 3181, 3311
Eco47III AGCGCT 1 cut(s) 3151
Eco57I CTGAAG 6 cut(s) 214, 225, 2390, 2628, 3432, 3807
Eco81I CCTNAGG 1 cut(s) 2157
Eco88I CYCGRG 1 cut(s) 2244
EcoNI CCTNNNNNAGG 3 cut(s) 2400, 2709, 4459
EcoO109I RGGNCCY 1 cut(s) 2738
EcoRI GAATTC 3 cut(s) 380, 3123, 4347
EcoRII CCWGG 4 cut(s) 2018, 2631, 2725, 2964
EcoRV GATATC 2 cut(s) 1681, 3265
EcoT14I CCWWGG 1 cut(s) 3696
EcoT22I ATGCAT 2 cut(s) 1327, 2842
EcoT38I GRGCYC 2 cut(s) 2535, 2741
ErhI CCWWGG 1 cut(s) 3696
Esp3I CGTCTC 1 cut(s) 1084
FalI AAGNNNNNCTT 8 cut(s) 3012, 3044, 3876, 3908, 4225, 4257, 4508, 4540
FaqI GGGAC 2 cut(s) 1957, 3297
FauNDI CATATG 5 cut(s) 1316, 1720, 2050, 2262, 4315
FbaI TGATCA 2 cut(s) 3066, 3232
FblI GTMKAC 2 cut(s) 4186, 4364
FokI GGATG 7 cut(s) 98, 922, 1868, 2310, 3844, 3954, 4477
FriOI GRGCYC 2 cut(s) 2535, 2741
FspBI CTAG 8 cut(s) 627, 699, 1359, 1437, 3057, 3120, 3188, 3198
FspI TGCGCA 1 cut(s) 3671
GlaI GCGC 5 cut(s) 1598, 3087, 3151, 3671, 3764
GsaI CCCAGC 2 cut(s) 804, 2541
GsuI CTGGAG 3 cut(s) 2175, 3411, 3987
HaeII RGCGCY 2 cut(s) 3089, 3153
HapII CCGG 4 cut(s) 22, 31, 298, 3174
HgaI GACGC 2 cut(s) 1618, 1785
HhaI GCGC 5 cut(s) 1599, 3088, 3152, 3672, 3765
Hin6I GCGC 5 cut(s) 1597, 3086, 3150, 3670, 3763
HinP1I GCGC 5 cut(s) 1597, 3086, 3150, 3670, 3763
HincII GTYRAC 5 cut(s) 558, 1713, 2598, 4062, 4187
HindII GTYRAC 5 cut(s) 558, 1713, 2598, 4062, 4187
HindIII AAGCTT 6 cut(s) 335, 1223, 2372, 3726, 3890, 4522
HpaI GTTAAC 1 cut(s) 4062
HpaII CCGG 4 cut(s) 22, 31, 298, 3174
HphI GGTGA 6 cut(s) 20, 1347, 1781, 2384, 3489, 4037
Hpy99I CGWCG 1 cut(s) 1779
HspAI GCGC 5 cut(s) 1597, 3086, 3150, 3670, 3763
KroI GCCGGC 1 cut(s) 21
KroNI GCCGGC 1 cut(s) 23
Ksp22I TGATCA 2 cut(s) 3066, 3232
KspAI GTTAAC 1 cut(s) 4062
LguI GCTCTTC 2 cut(s) 2193, 2604
LmnI GCTCC 7 cut(s) 1243, 1265, 2069, 2705, 4426, 4604, 4990
MaeI CTAG 8 cut(s) 627, 699, 1359, 1437, 3057, 3120, 3188, 3198
MflI RGATCY 5 cut(s) 1210, 2044, 2635, 3692, 4568
MhlI GDGCHC 6 cut(s) 192, 1499, 2535, 2616, 2741, 4043
MlsI TGGCCA 1 cut(s) 3684
MluI ACGCGT 1 cut(s) 1604
MluNI TGGCCA 1 cut(s) 3684
MlyI GAGTC 6 cut(s) 64, 617, 717, 1745, 1939, 2929
MmeI TCCRAC 5 cut(s) 1404, 1838, 2452, 3514, 4605
Mox20I TGGCCA 1 cut(s) 3684
Mph1103I ATGCAT 2 cut(s) 1327, 2842
MroNI GCCGGC 1 cut(s) 21
MroXI GAANNNNTTC 4 cut(s) 1045, 2679, 3024, 3345
MscI TGGCCA 1 cut(s) 3684
MslI CAYNNNNRTG 4 cut(s) 1767, 2444, 3940, 4197
Msp20I TGGCCA 1 cut(s) 3684
MspA1I CMGCKG 3 cut(s) 1492, 2388, 4973
MspCI CTTAAG 2 cut(s) 289, 584
MspI CCGG 4 cut(s) 22, 31, 298, 3174
MspR9I CCNGG 4 cut(s) 2020, 2633, 2727, 2966
Mva1269I GAATGC 2 cut(s) 2912, 4996
MvaI CCWGG 4 cut(s) 2020, 2633, 2727, 2966
MvnI CGCG 1 cut(s) 1606
NaeI GCCGGC 1 cut(s) 23
NdeI CATATG 5 cut(s) 1316, 1720, 2050, 2262, 4315
NgoMIV GCCGGC 1 cut(s) 21
NlaIV GGNNCC 6 cut(s) 296, 1540, 2637, 2739, 2740, 3313
NmuCI GTSAC 7 cut(s) 248, 609, 685, 1743, 2390, 2882, 3495
NsbI TGCGCA 1 cut(s) 3671
NsiI ATGCAT 2 cut(s) 1327, 2842
PaeI GCATGC 3 cut(s) 2004, 3670, 4433
PagI TCATGA 2 cut(s) 2931, 5009
PaqCI CACCTGC 1 cut(s) 437
PceI AGGCCT 1 cut(s) 4865
PciI ACATGT 3 cut(s) 392, 2752, 4181
PciSI GCTCTTC 2 cut(s) 2193, 2604
PctI GAATGC 2 cut(s) 2912, 4996
PdiI GCCGGC 1 cut(s) 23
PdmI GAANNNNTTC 4 cut(s) 1045, 2679, 3024, 3345
PfeI GAWTC 9 cut(s) 737, 1788, 2321, 2591, 3194, 3488, 3515, 3718, 4238
Pfl23II CGTACG 1 cut(s) 1074
PflMI CCANNNNNTGG 2 cut(s) 1037, 2025
PfoI TCCNGGA 1 cut(s) 2725
PinAI ACCGGT 2 cut(s) 30, 3173
PleI GAGTC 6 cut(s) 63, 616, 716, 1745, 1939, 2929
PpsI GAGTC 6 cut(s) 63, 616, 716, 1745, 1939, 2929
PscI ACATGT 3 cut(s) 392, 2752, 4181
PshAI GACNNNNGTC 1 cut(s) 1628
PshBI ATTAAT 2 cut(s) 2459, 3741
PsiI TTATAA 2 cut(s) 1550, 3590
Psp1406I AACGTT 1 cut(s) 3831
Psp6I CCWGG 4 cut(s) 2018, 2631, 2725, 2964
PspFI CCCAGC 2 cut(s) 800, 2537
PspGI CCWGG 4 cut(s) 2018, 2631, 2725, 2964
PspLI CGTACG 1 cut(s) 1074
PspN4I GGNNCC 6 cut(s) 296, 1540, 2637, 2739, 2740, 3313
PspOMI GGGCCC 1 cut(s) 2737
PspPI GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3181, 3311, 3607
PstNI CAGNNNCTG 3 cut(s) 3721, 3965, 4904
PsuI RGATCY 5 cut(s) 1210, 2044, 2635, 3692, 4568
PvuII CAGCTG 3 cut(s) 1492, 2388, 4973
RsaI GTAC 9 cut(s) 489, 915, 1076, 1305, 2903, 3004, 4017, 4698, 4896
RsaNI GTAC 9 cut(s) 488, 914, 1075, 1304, 2902, 3003, 4016, 4697, 4895
RseI CAYNNNNRTG 4 cut(s) 1767, 2444, 3940, 4197
SalI GTCGAC 1 cut(s) 4185
SapI GCTCTTC 2 cut(s) 2193, 2604
Sau96I GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3181, 3311, 3607
ScaI AGTACT 1 cut(s) 2903
SchI GAGTC 6 cut(s) 64, 617, 717, 1745, 1939, 2929
ScrFI CCNGG 4 cut(s) 2020, 2633, 2727, 2966
SduI GDGCHC 6 cut(s) 192, 1499, 2535, 2616, 2741, 4043
SfcI CTRYAG 2 cut(s) 3993, 4020
SfiI GGCCNNNNNGGCC 1 cut(s) 953
SinI GGWCC 4 cut(s) 461, 1738, 3181, 3311
SmiMI CAYNNNNRTG 4 cut(s) 1767, 2444, 3940, 4197
SphI GCATGC 3 cut(s) 2004, 3670, 4433
SseBI AGGCCT 1 cut(s) 4865
SsiI CCGC 6 cut(s) 1086, 1736, 2339, 2629, 4655, 4981
SspI AATATT 4 cut(s) 1833, 2457, 2650, 4210
SspMI CTAG 8 cut(s) 627, 699, 1359, 1437, 3057, 3120, 3188, 3198
StuI AGGCCT 1 cut(s) 4865
StyD4I CCNGG 4 cut(s) 2018, 2631, 2725, 2964
StyI CCWWGG 1 cut(s) 3696
TaqI TCGA 7 cut(s) 1002, 1774, 3217, 3286, 3518, 3986, 4186
TaqII GACCGA 1 cut(s) 156
TatI WGTACW 2 cut(s) 2901, 3002
TauI GCSGC 2 cut(s) 4657, 4984
TfiI GAWTC 9 cut(s) 737, 1788, 2321, 2591, 3194, 3488, 3515, 3718, 4238
TseFI GTSAC 7 cut(s) 248, 609, 685, 1743, 2390, 2882, 3495
Tsp45I GTSAC 7 cut(s) 248, 609, 685, 1743, 2390, 2882, 3495
Van91I CCANNNNNTGG 2 cut(s) 1037, 2025
Vha464I CTTAAG 2 cut(s) 289, 584
VpaK11BI GGWCC 4 cut(s) 461, 1738, 3181, 3311
VspI ATTAAT 2 cut(s) 2459, 3741
XagI CCTNNNNNAGG 3 cut(s) 2400, 2709, 4459
XbaI TCTAGA 1 cut(s) 3119
XcmI CCANNNNNNNNNTGG 1 cut(s) 4072
XmiI GTMKAC 2 cut(s) 4186, 4364
XmnI GAANNNNTTC 4 cut(s) 1045, 2679, 3024, 3345
XspI CTAG 8 cut(s) 627, 699, 1359, 1437, 3057, 3120, 3188, 3198
ZrmI AGTACT 1 cut(s) 2903
Zsp2I ATGCAT 2 cut(s) 1327, 2842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.