RLG00000026892

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003903
Physical Location & Seq
Forward (+)
6806358 .. 6816591
10234 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026892

Sequence Viewer

Length: 5013 bp
ATGGGTACAAGGAAGCAAAACCGGCCTCACCGGTCGGGTGGGATTGTATTAGAGAGTCAAGCAACTGCTGGCAGGGGAGAATTGGATGAAGGGAGGCTTTTAAATGGGGAAGTGCAACAGAATGACATAGTTGAAGTTGATAGACCGTACTTTGTGAAAGTTGATCGGTCTAATTGGGGATCAGATGAGCACCTTGATATTGCTGAGGTTGTTTTAACAGATTTGACTTTTGGAGAAGGGTTTTGCAGTGATGTTCTTAATGGGGGTTTTAATCGCAATAGTTACTCCTTGAGGTTCCGGTTATCTAATGTGAAGGAGTTCATTGGTTGTATTAAGCTTGGTCATTGGCCTTTGTTGTCCTCTGCTGAAATATCTTTGGAATTCGTTAAAACATGTTCAAGCGAGGCTATGGACATGGAGAAGGACACATGCTCAGTAATCTTGTCAGGGAGTTTTGACGGGCCAGATGAAGGTATTTCTGGTCTGGTTCACTTGGCAAGTTTGAAGTTTATGACACTGAGGCCAGCTCTGTCAATTGGTTTTGTGAATGATACGTCAACCATCAGGGTCAGGGTGGAGATACTTAAGAGTGCATTTGATGCTTGTGAGTCACTTCTGGACAATACTAGGCAAGTGTGGAAAAAGAGTATGATGAATGTCATGGCTTGGCTACACCCAGAAGTAGTGACTTCCGAGGCTAGGTATGGAGTCAGTAAATCAACAGACATGGAAATTGATTTGCATACTGACACTGGACATGCTAATTCAAATCATAGTCGACATGCAAGGTTTGATGTTGCTGGGTTCTATGAAGCCATTAAACCCTCAAAAGCAGATCCAATGCTTCAAGATGTCCTGCCTGATTTGCTTCCTGAATTGAAACCATATCAGCGCCGGGCAGCTTACTGGATGGTACAGAGGGAGAAACGCGATGCAGAAAGTCTGGCCCAAGAGGCCAAAAGTCAGTTTATGTCGCCTTTATGTTTGCCCTTGCAATTTCTCGACAGATGTTCAAAAATGTTCTACAACCCATTCAGTGGAAATGTTTCACTGCACCAAGAGCATTTTTCAACATACGTCTCTGGTGGTATTCTTGCTGATGAGATGGGCATGGGGAAAACTGTTGAACTTCTCGCTTGCATCTTTGCTCATCAGAAGTCAGCAGATGAAGACCCCATATTTGCTGATGCTGAAATTGAAGATACCGAGGATCTGAAAATAAATCTTAAAAGATTAAAAATGGAGCGAGTTGAATGTATATGTGGAGCTGTTAGTGAAAGTAGCAGATACAGAGGATTATGGGTACAGTGTGACATATGTGATGCATGGCAACATGCCAATTGTGTGGGCTATTCTCCTAGAGGAAAGACTATAAAATCCAACGAGGCTTCTAACGAAAGAGAACATGAAAAGAGTTCGGTGGTTAAGAAGTATACTAGAAAGAAGAACACAACCACAATAGACGTGAGAGATGAGGAATATATATGCCAGCTGTGCTCAGAACTGATACAGGCCACTAATTCTCCTGTTGCTACTGGTGCCACTCTTATAATCTGTCCAGCTTCTATAATGCCCCAGTGGTATTCTGAAATTATGCACCATACGCGTTCAGGTTCTCTAAAGACTTGCGTCTATGAAGGTGTGAGAGAGGCGTCTTTTTCAAACACATCTGTAATTGATATCAGTGAACTCATCAGTGCTGACATTGTGTTGACAACATATGATGTGCTTAAGGCGGACCTGTCACATGACTCTGATAGGCATGAAGGTGATCGACGCTTAATGAGATTCCAAAAGAGATACCCTGTCGTTCCAACTACTCTAACCAGAATATTCTGGTGGAGAATTTGTTTGGATGAGGCTCAGATGGTGGAGAGTAATGCTGGTGCTGCAACAGAAATGGCTATGCGACTGTATGCGAAGCATCGTTGGTGCATAACGGGGACTCCTATACAACGAAAACTTGATGACTTATATGGACTTCTACGGTTTCTTAAAGCATGCCCTTTCAATGCTTCGAGGTGGTGGATTGAAGTTATACGAGATCCATATGAGAGGAGAGATGCAGGAGCTATCGAATTTACACATAAATTCTTTAAAGAAATCATGTGGCGATCTTCAAAAGTGCATGTTGCAGATGAGTTGCAGCTTCCACCTCAGGAAGAATGTCTCTCTTGGCTCACTTTATCTCCAGTTGAAGAGCATTTTTACCAGAGGCAACATGAAACTTGTGTGAGTTATGCCCGAGAAGTCATTGAAAGTTTGAAAGATGATATTATGAAGAGAAAAGTCGGAGGATGCTCAGCAGCTAAATCATCAGATTCTTTCATCACACATGCGGAGGCAGGAAAGCTGCTGAACACACTTCTAAAGCTTCGCCAAGCCAGCTGTCACCCTCAAGTTGGAAGCTCAGGCCTGCGTTCTCTCCAGCAGTCTCCCATGACTATGGAGGAAATATTGATGGTTCTTGTAAGTAAAACGAAGATAGAAGGAGAGGAAGCTCTCAGAAGGTTAGTTGTTGCTCTTAATGGGCTCGCTGGGATAGCTGTAATTGAGCAAAATTTTACTCAAGCTGTATCACTCTATAAGGAATCGTTGACTTTAGCTGAAGAGCACGCTGAGGATTTCCGCTTGGATCCTTTGTTGAGTATTCACATTCATCATAATCTTGCTGAAATACTCCCATTAGCAACAAGCCGCTCCCCTTCAAAAGCAGAACATATTCCTGGGACAATGGGCCCCTGTGACGAACATGTAGCGAAGAGGCGAAAACTGAGTGGGGAAGATAACTCATCAGAGAATGACTTTTCTATTGCCCAAGAATATAATAACGTGTCCCATGCATCGTGTACTTCCTTCACTGATGTATCTTTGAGAACAATCTGTGACAATCTTAAACAAAAGTACTTATCTGCATTCAGTTCAAAGTTAATCATGACTCAGCAAGAGTTTAAGAAGTCTTACACCCAGGTTTGTAGCACAATTAGTGAGATGAAAGATGTAAGTACAGTTTGGTGGTTGGAAGCTCTTCTTCATGCTGAAAAGAATAGTGACATTTCTAGTCTCTTGATCAGAAAGATTGAAGAGGTGCTCATAGGAACTCTAAACAATTCAAAGTCATCTAAAATTCCATCTCGTTTACGAAGCATAAGCGCTCTAAAGTATCATATGCAGACCGGCTTGGACCAACTAGAAGAATCTAGAAAAATATTACTTGATCGACTTTTAGAAATTGATCAGACAATGGAGAAGCCAAAAGAGGAGGATATCCAGCGTGTGAGATACTGTCGAAATTGCAAAGCTAACGATGGTGGTCCCTTATGTGTTCTTTGTGAAGTAGATGAATTATTTCGGGGTTATGAAGCAAGGCTCTTTCGTCTTGAGAAAGTATCTGGAGGGATTGCTACCTCTGCTGAAGAGGCTGTGGATTTGCAGAAGAAGAACTCTGCACTTAATCGTTTCTATCAGAATTTGTCACAGCCGATTAAGGATTCAACATCACCCAAATCTAATCAAGAATCAAAGAAGAGAGATGTTGGAAAAGTTGTGGTTTCAAAATCCCCATCTGAATTAGAGGTTGTTCTTGGAGTTATAAAAAGCCATTGCAAGGCCCATTTAGGAAAGGAAGGTATATCAGAAGCTACCAAGCACCTACACATTCTGGAGGGCATGCGCAAGGAGTATGGCCATGCAAGATCCTTGGCAATAGCTCAAGCTCAGATTCTGCAAGCCTGTGATGAAATTAATATGGCAACTACAAGGTTGCGCTTGAGTGAAGATGAGAATGATAAATCTCTTGATGCATTAAGTGAAGATGAATTACCTTCAGCTAATGTTTTATACACCAGTGAAAAGTTCGCATCCTTGAGCTTGTTGTCTTGTATAAAAGGGAAACTTCGTTATTTGAAGGGTTTGGTAGAAGCTAAACAAAAAATGCCATTTGGAAGTCCAAATCATTCCCCAGTAGAAGAGGAAGCCACCATATCAACTTCAACAGAACAGAGAAAGGAATGCATACCCACAGGTGATAAAGAAACGTGCCCAGTTTGTCAAGAACAATTAACCATTCAAAAGATGGTTTTCCCTTGTGGACACCTTACTTGCTGTAAATGTTTGTTTGCAATTACTGAGAGACTACTCAATGGGAAAAAGTTTCAAGATAAGTGGGTAAAATGCCCGACATGTCGACAGCACACTGATGTTGCAAATATTGCTTTTGCTGATGATGGACATAGCGAATCTTCTGGTTCTGCTGGGCTTCATGCAATCCAAAGTCGTGAAGAGTGTGAAGCATCTATTATAGTTCAAGGTTCATATGGAACAAAGATTGAAGCAGTTACAAGAAGAATTCTGTGGATAAAGTCTACAGATCCAGAGGCTAAAGTTCTTGTTTTCTCAAGCTGGAATGATGTCCTTGACGTATTGGAGCATGCCTTCAGCGCTAATGGCATTACCTTTATAAGGATGAAAGGAGGAAGGAAATCACAAGTTGCCATTAGTGAATTTAAAGGAGAAAAGAGAAGCTTAACAGGAAATCACAAGTTGCATGGCCGAAACCCAGAAGGAAGATCTATTCAAGTGTTGCTGCTCTTAATTCAACACGGAGCAAATGGCCTCAATCTGTTGGAAGCGAAACATGTTATTCTTATTGAGCCGCTTCTCAATCCAGCAGCAGAAGCACAAGCCATCAGCAGAGTACACCGAATCGGACAGAAAAATAAGACCCTCGCTCATCGTTTCATAGTTAAAGGCACAGTTGAAGAGAGCATATACAAGCTCAACAAAAGCAGGAACAGCACTTCCTTCATTAGTGGTAACACAAAGAACCAAGATCAGCCCTTATTGACACTAAAAGACATCGAGGCCTTATTTTCAACAGTGCCATCATTAACTGTACCAGAGACTGATGAAAGGCCAACTGAAAGTGAAAGTTTACGACATTTGCCACCTTCTAGGGCTGCTGCCATTGCAGCTGAGAAGCGGCTCAAGGAGCAGCATTCATGTTCTTCATGA

Protein Analysis

1671

Amino Acids

187.35

Weight (kDa)

6.44

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 296 - 800 3.4e-63 SNF2-related domain
SHPRH_helical-1st PF21325 816 - 898 1.4e-24 E3 ubiquitin-protein ligase SHPRH, first helical domain
SHPRH_helical-2nd PF21324 1003 - 1127 2.7e-07 E3 ubiquitin-protein ligase SHPRH, second helical domain
Helicase_C PF00271 1444 - 1570 5.3e-07 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1550, 3593, 4460
Acc16I TGCGCA 1 cut(s) 3674
AccB1I GGYRCC 1 cut(s) 1538
AccB7I CCANNNNNTGG 1 cut(s) 1037
AccBSI CCGCTC 1 cut(s) 2700
AccI GTMKAC 4 cut(s) 778, 1433, 4186, 4364
AccII CGCG 2 cut(s) 930, 1606
AciI CCGC 6 cut(s) 1736, 2339, 2629, 2698, 4655, 4981
AclWI GGATC 8 cut(s) 187, 830, 1218, 2039, 2630, 2643, 3690, 4364
AcoI YGGCCR 2 cut(s) 3685, 4549
AcsI RAATTY 9 cut(s) 380, 1845, 2078, 2090, 2560, 3126, 3469, 4347, 4502
AcuI CTGAAG 4 cut(s) 2628, 3435, 3810, 4420
AcyI GRCGYC 1 cut(s) 1652
AfaI GTAC 9 cut(s) 7, 149, 915, 1305, 2851, 2906, 3007, 4698, 4896
AfeI AGCGCT 2 cut(s) 3154, 4441
AfiI CCNNNNNNNGG 6 cut(s) 470, 699, 1037, 2402, 3607, 4461
AflII CTTAAG 2 cut(s) 584, 1730
AflIII ACRYGT 6 cut(s) 392, 1604, 2752, 2832, 4181, 4636
AgeI ACCGGT 1 cut(s) 30
AjiI CACGTC 1 cut(s) 1465
AjnI CCWGG 2 cut(s) 2725, 2967
AloI GAACNNNNNNTCC 2 cut(s) 4784, 4816
Alw21I GWGCWC 4 cut(s) 192, 1499, 2616, 3093
Alw26I GTCTC 6 cut(s) 1084, 2174, 2439, 3068, 4126, 4895
AlwI GGATC 8 cut(s) 187, 830, 1218, 2039, 2630, 2643, 3690, 4364
AlwNI CAGNNNCTG 2 cut(s) 3724, 4904
Ama87I CYCGRG 1 cut(s) 2244
Aor51HI AGCGCT 2 cut(s) 3154, 4441
ApaI GGGCCC 1 cut(s) 2741
ApoI RAATTY 9 cut(s) 380, 1845, 2078, 2090, 2560, 3126, 3469, 4347, 4502
ArsI GACNNNNNNTTYG 2 cut(s) 1163, 1195
AseI ATTAAT 1 cut(s) 3744
AsiGI ACCGGT 1 cut(s) 30
Asp700I GAANNNNTTC 5 cut(s) 317, 1045, 2721, 3027, 3348
AspLEI GCGC 5 cut(s) 894, 3155, 3675, 3768, 4442
AspS9I GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3184, 3314, 3610
AsuC2I CCSGG 1 cut(s) 896
AsuHPI GGTGA 5 cut(s) 20, 1781, 2384, 3492, 4037
AvaI CYCGRG 1 cut(s) 2244
AvaII GGWCC 3 cut(s) 1738, 3184, 3314
AxyI CCTNAGG 1 cut(s) 2157
BaeGI GKGCMC 2 cut(s) 2741, 4043
BaeI ACNNNNGTAYC 2 cut(s) 2850, 2883
BalI TGGCCA 1 cut(s) 3687
BamHI GGATCC 1 cut(s) 2635
BanI GGYRCC 1 cut(s) 1538
BanII GRGCYC 2 cut(s) 2535, 2741
BarI GAAGNNNNNNTAC 2 cut(s) 3804, 3836
BbsI GAAGAC 1 cut(s) 1176
Bbv12I GWGCWC 4 cut(s) 192, 1499, 2616, 3093
BbvCI CCTCAGC 2 cut(s) 204, 2619
BciT130I CCWGG 2 cut(s) 2727, 2969
BclI TGATCA 2 cut(s) 3069, 3235
BcnI CCSGG 1 cut(s) 896
BcoDI GTCTC 6 cut(s) 1084, 2174, 2439, 3068, 4126, 4895
BfaI CTAG 8 cut(s) 627, 699, 1359, 1437, 3060, 3191, 3201, 4953
BfmI CTRYAG 1 cut(s) 4365
BfoI RGCGCY 3 cut(s) 895, 3156, 4443
BfrI CTTAAG 2 cut(s) 584, 1730
BglI GCCNNNNNGGC 1 cut(s) 953
BglII AGATCT 1 cut(s) 4568
BlpI GCTNAGC 1 cut(s) 2302
BmcAI AGTACT 1 cut(s) 2906
Bme1390I CCNGG 3 cut(s) 896, 2727, 2969
Bme18I GGWCC 3 cut(s) 1738, 3184, 3314
BmeT110I CYCGRG 1 cut(s) 2244
BmgBI CACGTC 1 cut(s) 1465
BmgT120I GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3184, 3314, 3610
BmiI GGNNCC 6 cut(s) 296, 1540, 2637, 2739, 2740, 3316
BmrFI CCNGG 3 cut(s) 896, 2727, 2969
BmrI ACTGGG 3 cut(s) 1570, 3956, 4037
BmuI ACTGGG 3 cut(s) 1570, 3956, 4037
BoxI GACNNNNGTC 1 cut(s) 1628
BpiI GAAGAC 1 cut(s) 1176
BplI GAGNNNNNCTC 2 cut(s) 511, 543
BpmI CTGGAG 4 cut(s) 2175, 2411, 3414, 3683
Bpu10I CCTNAGC 3 cut(s) 204, 2410, 2619
Bpu1102I GCTNAGC 1 cut(s) 2302
BpuEI CTTGAG 9 cut(s) 310, 2382, 2553, 3401, 3696, 3790, 3886, 4381, 4970
BpuMI CCSGG 1 cut(s) 896
BsaHI GRCGYC 1 cut(s) 1652
BsaJI CCNNGG 5 cut(s) 693, 1206, 2726, 2967, 3699
BsaWI WCCGGW 2 cut(s) 30, 297
BsaXI ACNNNNNCTCC 8 cut(s) 1507, 1537, 1834, 1864, 1930, 1960, 2173, 2203
Bsc4I CCNNNNNNNGG 6 cut(s) 470, 699, 1037, 2402, 3607, 4461
Bse118I RCCGGY 3 cut(s) 21, 30, 3176
Bse1I ACTGG 8 cut(s) 757, 911, 1540, 1576, 2192, 3846, 3962, 4043
Bse21I CCTNAGG 1 cut(s) 2157
Bse3DI GCAATG 2 cut(s) 3601, 4965
BseBI CCWGG 2 cut(s) 2727, 2969
BseDI CCNNGG 5 cut(s) 693, 1206, 2726, 2967, 3699
BseGI GGATG 6 cut(s) 91, 915, 1861, 2303, 3860, 4470
BseLI CCNNNNNNNGG 6 cut(s) 470, 699, 1037, 2402, 3607, 4461
BseMI GCAATG 2 cut(s) 3601, 4965
BseNI ACTGG 8 cut(s) 757, 911, 1540, 1576, 2192, 3846, 3962, 4043
BseRI GAGGAG 2 cut(s) 2071, 3275
BseSI GKGCMC 2 cut(s) 2741, 4043
BseYI CCCAGC 3 cut(s) 800, 2537, 4253
BsgI GTGCAG 2 cut(s) 1037, 3432
Bsh1236I CGCG 2 cut(s) 930, 1606
Bsh1285I CGRYCG 1 cut(s) 35
BshNI GGYRCC 1 cut(s) 1538
BshTI ACCGGT 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 35
BsiHKAI GWGCWC 4 cut(s) 192, 1499, 2616, 3093
BsiHKCI CYCGRG 1 cut(s) 2244
BsiSI CCGG 5 cut(s) 22, 31, 298, 895, 3177
BslFI GGGAC 4 cut(s) 1957, 2743, 2821, 3300
BslI CCNNNNNNNGG 6 cut(s) 470, 699, 1037, 2402, 3607, 4461
BsmAI GTCTC 6 cut(s) 1084, 2174, 2439, 3068, 4126, 4895
BsmBI CGTCTC 1 cut(s) 1084
BsmFI GGGAC 4 cut(s) 1957, 2743, 2821, 3300
BsmI GAATGC 3 cut(s) 2915, 4016, 4996
BsoBI CYCGRG 1 cut(s) 2244
Bsp120I GGGCCC 1 cut(s) 2737
Bsp1286I GDGCHC 7 cut(s) 192, 1499, 2535, 2616, 2741, 3093, 4043
Bsp1720I GCTNAGC 1 cut(s) 2302
BspACI CCGC 6 cut(s) 1736, 2339, 2629, 2698, 4655, 4981
BspFNI CGCG 2 cut(s) 930, 1606
BspHI TCATGA 2 cut(s) 2934, 5009
BspLI GGNNCC 6 cut(s) 296, 1540, 2637, 2739, 2740, 3316
BspPI GGATC 8 cut(s) 187, 830, 1218, 2039, 2630, 2643, 3690, 4364
BspQI GCTCTTC 3 cut(s) 2193, 2604, 3033
BspT107I GGYRCC 1 cut(s) 1538
BspTI CTTAAG 2 cut(s) 584, 1730
BsrBI CCGCTC 1 cut(s) 2700
BsrDI GCAATG 2 cut(s) 3601, 4965
BsrFI RCCGGY 3 cut(s) 21, 30, 3176
BsrI ACTGG 8 cut(s) 757, 911, 1540, 1576, 2192, 3846, 3962, 4043
BssAI RCCGGY 3 cut(s) 21, 30, 3176
BssECI CCNNGG 5 cut(s) 693, 1206, 2726, 2967, 3699
BssNAI GTATAC 1 cut(s) 1434
BssNI GRCGYC 1 cut(s) 1652
BssT1I CCWWGG 1 cut(s) 3699
Bst1107I GTATAC 1 cut(s) 1434
Bst2UI CCWGG 2 cut(s) 2727, 2969
BstACI GRCGYC 1 cut(s) 1652
BstAFI CTTAAG 2 cut(s) 584, 1730
BstAPI GCANNNNNTGC 2 cut(s) 599, 4211
BstENI CCTNNNNNAGG 1 cut(s) 4459
BstF5I GGATG 6 cut(s) 91, 915, 1861, 2303, 3860, 4470
BstFNI CGCG 2 cut(s) 930, 1606
BstH2I RGCGCY 3 cut(s) 895, 3156, 4443
BstHHI GCGC 5 cut(s) 894, 3155, 3675, 3768, 4442
BstMAI GTCTC 6 cut(s) 1084, 2174, 2439, 3068, 4126, 4895
BstMCI CGRYCG 1 cut(s) 35
BstNI CCWGG 2 cut(s) 2727, 2969
BstPAI GACNNNNGTC 1 cut(s) 1628
BstSCI CCNGG 3 cut(s) 894, 2725, 2967
BstSFI CTRYAG 1 cut(s) 4365
BstSLI GKGCMC 2 cut(s) 2741, 4043
BstUI CGCG 2 cut(s) 930, 1606
BstV2I GAAGAC 1 cut(s) 1176
BstX2I RGATCY 7 cut(s) 835, 1210, 2044, 2635, 3695, 4369, 4568
BstXI CCANNNNNNTGG 2 cut(s) 1345, 2446
BstYI RGATCY 7 cut(s) 835, 1210, 2044, 2635, 3695, 4369, 4568
BstZ17I GTATAC 1 cut(s) 1434
Bsu36I CCTNAGG 1 cut(s) 2157
BtgZI GCGATG 1 cut(s) 945
BtrI CACGTC 1 cut(s) 1465
BtsCI GGATG 6 cut(s) 91, 915, 1861, 2303, 3860, 4470
BtsI GCAGTG 2 cut(s) 253, 1049
CaiI CAGNNNCTG 2 cut(s) 3724, 4904
CciI TCATGA 2 cut(s) 2934, 5009
CfoI GCGC 5 cut(s) 894, 3155, 3675, 3768, 4442
Cfr10I RCCGGY 3 cut(s) 21, 30, 3176
Cfr13I GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3184, 3314, 3610
CseI GACGC 3 cut(s) 1618, 1641, 1785
Csp6I GTAC 9 cut(s) 6, 148, 914, 1304, 2850, 2905, 3006, 4697, 4895
CspAI ACCGGT 1 cut(s) 30
CspCI CAANNNNNGTGG 2 cut(s) 4145, 4180
CviQI GTAC 9 cut(s) 6, 148, 914, 1304, 2850, 2905, 3006, 4697, 4895
DraI TTTAAA 3 cut(s) 102, 2098, 4507
EaeI YGGCCR 2 cut(s) 3685, 4549
EciI GGCGGA 1 cut(s) 1751
Eco130I CCWWGG 1 cut(s) 3699
Eco147I AGGCCT 2 cut(s) 2415, 4865
Eco24I GRGCYC 2 cut(s) 2535, 2741
Eco32I GATATC 2 cut(s) 1681, 3268
Eco47I GGWCC 3 cut(s) 1738, 3184, 3314
Eco47III AGCGCT 2 cut(s) 3154, 4441
Eco57I CTGAAG 4 cut(s) 2628, 3435, 3810, 4420
Eco81I CCTNAGG 1 cut(s) 2157
Eco88I CYCGRG 1 cut(s) 2244
EcoNI CCTNNNNNAGG 1 cut(s) 4459
EcoO109I RGGNCCY 1 cut(s) 2738
EcoRI GAATTC 2 cut(s) 380, 4347
EcoRII CCWGG 2 cut(s) 2725, 2967
EcoRV GATATC 2 cut(s) 1681, 3268
EcoT14I CCWWGG 1 cut(s) 3699
EcoT22I ATGCAT 4 cut(s) 1327, 2845, 3805, 4016
EcoT38I GRGCYC 2 cut(s) 2535, 2741
ErhI CCWWGG 1 cut(s) 3699
Esp3I CGTCTC 1 cut(s) 1084
FalI AAGNNNNNCTT 8 cut(s) 81, 113, 3015, 3047, 3879, 3911, 4508, 4540
FaqI GGGAC 4 cut(s) 1957, 2743, 2821, 3300
FauNDI CATATG 5 cut(s) 1316, 1720, 2050, 3168, 4315
FbaI TGATCA 2 cut(s) 3069, 3235
FblI GTMKAC 4 cut(s) 778, 1433, 4186, 4364
FokI GGATG 6 cut(s) 98, 922, 1868, 2310, 3847, 4477
FriOI GRGCYC 2 cut(s) 2535, 2741
FspBI CTAG 8 cut(s) 627, 699, 1359, 1437, 3060, 3191, 3201, 4953
FspI TGCGCA 1 cut(s) 3674
GlaI GCGC 5 cut(s) 893, 3154, 3674, 3767, 4441
GsaI CCCAGC 3 cut(s) 804, 2541, 4257
GsuI CTGGAG 4 cut(s) 2175, 2411, 3414, 3683
HaeII RGCGCY 3 cut(s) 895, 3156, 4443
HapII CCGG 5 cut(s) 22, 31, 298, 895, 3177
HgaI GACGC 3 cut(s) 1618, 1641, 1785
HhaI GCGC 5 cut(s) 894, 3155, 3675, 3768, 4442
Hin1I GRCGYC 1 cut(s) 1652
Hin6I GCGC 5 cut(s) 892, 3153, 3673, 3766, 4440
HinP1I GCGC 5 cut(s) 892, 3153, 3673, 3766, 4440
HincII GTYRAC 5 cut(s) 558, 779, 1713, 2598, 4187
HindII GTYRAC 5 cut(s) 558, 779, 1713, 2598, 4187
HindIII AAGCTT 3 cut(s) 335, 2372, 4522
HpaII CCGG 5 cut(s) 22, 31, 298, 895, 3177
HphI GGTGA 5 cut(s) 20, 1781, 2384, 3492, 4037
Hpy99I CGWCG 1 cut(s) 1779
HpyCH4IV ACGT 6 cut(s) 554, 1077, 1464, 2832, 4037, 4419
HpySE526I ACGT 6 cut(s) 554, 1077, 1464, 2832, 4037, 4419
Hsp92I GRCGYC 1 cut(s) 1652
HspAI GCGC 5 cut(s) 892, 3153, 3673, 3766, 4440
Ksp22I TGATCA 2 cut(s) 3069, 3235
LguI GCTCTTC 3 cut(s) 2193, 2604, 3033
LmnI GCTCC 7 cut(s) 1243, 1265, 2069, 2705, 4426, 4604, 4990
MaeI CTAG 8 cut(s) 627, 699, 1359, 1437, 3060, 3191, 3201, 4953
MaeII ACGT 6 cut(s) 554, 1077, 1464, 2832, 4037, 4419
MbiI CCGCTC 1 cut(s) 2700
MfeI CAATTG 2 cut(s) 534, 1339
MflI RGATCY 7 cut(s) 835, 1210, 2044, 2635, 3695, 4369, 4568
MhlI GDGCHC 7 cut(s) 192, 1499, 2535, 2616, 2741, 3093, 4043
MlsI TGGCCA 1 cut(s) 3687
MluI ACGCGT 1 cut(s) 1604
MluNI TGGCCA 1 cut(s) 3687
MlyI GAGTC 6 cut(s) 64, 617, 717, 1745, 1939, 2932
MmeI TCCRAC 7 cut(s) 1404, 1838, 2272, 2383, 3000, 3517, 4605
Mox20I TGGCCA 1 cut(s) 3687
Mph1103I ATGCAT 4 cut(s) 1327, 2845, 3805, 4016
MroXI GAANNNNTTC 5 cut(s) 317, 1045, 2721, 3027, 3348
MscI TGGCCA 1 cut(s) 3687
MslI CAYNNNNRTG 3 cut(s) 1767, 2444, 4197
Msp20I TGGCCA 1 cut(s) 3687
MspA1I CMGCKG 3 cut(s) 1492, 2388, 4973
MspCI CTTAAG 2 cut(s) 584, 1730
MspI CCGG 5 cut(s) 22, 31, 298, 895, 3177
MspR9I CCNGG 3 cut(s) 896, 2727, 2969
MunI CAATTG 2 cut(s) 534, 1339
Mva1269I GAATGC 3 cut(s) 2915, 4016, 4996
MvaI CCWGG 2 cut(s) 2727, 2969
MvnI CGCG 2 cut(s) 930, 1606
NciI CCSGG 1 cut(s) 896
NdeI CATATG 5 cut(s) 1316, 1720, 2050, 3168, 4315
NlaIV GGNNCC 6 cut(s) 296, 1540, 2637, 2739, 2740, 3316
NmuCI GTSAC 9 cut(s) 609, 685, 1310, 1743, 2390, 2744, 2885, 3050, 3474
NsbI TGCGCA 1 cut(s) 3674
NsiI ATGCAT 4 cut(s) 1327, 2845, 3805, 4016
PaeI GCATGC 3 cut(s) 2004, 3673, 4433
PagI TCATGA 2 cut(s) 2934, 5009
PceI AGGCCT 2 cut(s) 2415, 4865
PciI ACATGT 4 cut(s) 392, 2752, 4181, 4636
PciSI GCTCTTC 3 cut(s) 2193, 2604, 3033
PctI GAATGC 3 cut(s) 2915, 4016, 4996
PdmI GAANNNNTTC 5 cut(s) 317, 1045, 2721, 3027, 3348
PfeI GAWTC 9 cut(s) 1788, 2321, 2591, 3197, 3491, 3518, 3721, 4238, 4704
PflMI CCANNNNNTGG 1 cut(s) 1037
PinAI ACCGGT 1 cut(s) 30
PleI GAGTC 6 cut(s) 63, 616, 716, 1745, 1939, 2932
PpsI GAGTC 6 cut(s) 63, 616, 716, 1745, 1939, 2932
PscI ACATGT 4 cut(s) 392, 2752, 4181, 4636
PshAI GACNNNNGTC 1 cut(s) 1628
PshBI ATTAAT 1 cut(s) 3744
PsiI TTATAA 3 cut(s) 1550, 3593, 4460
Psp6I CCWGG 2 cut(s) 2725, 2967
PspFI CCCAGC 3 cut(s) 800, 2537, 4253
PspGI CCWGG 2 cut(s) 2725, 2967
PspN4I GGNNCC 6 cut(s) 296, 1540, 2637, 2739, 2740, 3316
PspOMI GGGCCC 1 cut(s) 2737
PspPI GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3184, 3314, 3610
PstNI CAGNNNCTG 2 cut(s) 3724, 4904
PsuI RGATCY 7 cut(s) 835, 1210, 2044, 2635, 3695, 4369, 4568
PvuII CAGCTG 3 cut(s) 1492, 2388, 4973
RsaI GTAC 9 cut(s) 7, 149, 915, 1305, 2851, 2906, 3007, 4698, 4896
RsaNI GTAC 9 cut(s) 6, 148, 914, 1304, 2850, 2905, 3006, 4697, 4895
RseI CAYNNNNRTG 3 cut(s) 1767, 2444, 4197
SalI GTCGAC 2 cut(s) 777, 4185
SapI GCTCTTC 3 cut(s) 2193, 2604, 3033
Sau96I GGNCC 8 cut(s) 461, 946, 1738, 2737, 2738, 3184, 3314, 3610
ScaI AGTACT 1 cut(s) 2906
SchI GAGTC 6 cut(s) 64, 617, 717, 1745, 1939, 2932
ScrFI CCNGG 3 cut(s) 896, 2727, 2969
SduI GDGCHC 7 cut(s) 192, 1499, 2535, 2616, 2741, 3093, 4043
SfcI CTRYAG 1 cut(s) 4365
SfiI GGCCNNNNNGGCC 1 cut(s) 953
SinI GGWCC 3 cut(s) 1738, 3184, 3314
SmiMI CAYNNNNRTG 3 cut(s) 1767, 2444, 4197
SphI GCATGC 3 cut(s) 2004, 3673, 4433
SseBI AGGCCT 2 cut(s) 2415, 4865
SsiI CCGC 6 cut(s) 1736, 2339, 2629, 2698, 4655, 4981
SspI AATATT 4 cut(s) 1833, 2457, 3210, 4210
SspMI CTAG 8 cut(s) 627, 699, 1359, 1437, 3060, 3191, 3201, 4953
StuI AGGCCT 2 cut(s) 2415, 4865
StyD4I CCNGG 3 cut(s) 894, 2725, 2967
StyI CCWWGG 1 cut(s) 3699
TaiI ACGT 6 cut(s) 557, 1080, 1467, 2835, 4040, 4422
TaqI TCGA 9 cut(s) 778, 1002, 1774, 2018, 2076, 3220, 3289, 4186, 4860
TaqII GACCGA 1 cut(s) 156
TatI WGTACW 4 cut(s) 2849, 2904, 3005, 4696
TauI GCSGC 3 cut(s) 2700, 4657, 4984
TfiI GAWTC 9 cut(s) 1788, 2321, 2591, 3197, 3491, 3518, 3721, 4238, 4704
TseFI GTSAC 9 cut(s) 609, 685, 1310, 1743, 2390, 2744, 2885, 3050, 3474
Tsp45I GTSAC 9 cut(s) 609, 685, 1310, 1743, 2390, 2744, 2885, 3050, 3474
TspGWI ACGGA 1 cut(s) 4617
Van91I CCANNNNNTGG 1 cut(s) 1037
Vha464I CTTAAG 2 cut(s) 584, 1730
VpaK11BI GGWCC 3 cut(s) 1738, 3184, 3314
VspI ATTAAT 1 cut(s) 3744
XagI CCTNNNNNAGG 1 cut(s) 4459
XapI RAATTY 9 cut(s) 380, 1845, 2078, 2090, 2560, 3126, 3469, 4347, 4502
XbaI TCTAGA 1 cut(s) 3200
XcmI CCANNNNNNNNNTGG 1 cut(s) 4072
XmiI GTMKAC 4 cut(s) 778, 1433, 4186, 4364
XmnI GAANNNNTTC 5 cut(s) 317, 1045, 2721, 3027, 3348
XspI CTAG 8 cut(s) 627, 699, 1359, 1437, 3060, 3191, 3201, 4953
ZrmI AGTACT 1 cut(s) 2906
Zsp2I ATGCAT 4 cut(s) 1327, 2845, 3805, 4016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.