Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003903

Physical Location & Seq

Genomic Coordinates Reverse (-)

27322054 .. 27331319

Gene Structure 9266 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1169300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5037 bp

ATGGGTAGAAGGAAGCAAAGGAGGCCCCACCGATCCGGTGGGGTTGTATTGGAGAGTCGCAGCAATGCTGATCAGGCAGAATTGGATGGAATCAAGATTTCAAATGGGGAATCAGGAAAGAATGAACTGGATAAAGTTGATGAGCCGTATTTTGTGGAAGTTGATCGGTCTTGTTGGGTTTTTGACGAGCACCTTGATATTGCTGAAGTTGTTTTGACAAATTTGAATTTCGGAGAAGGGTTTTCTGGTGATGGATTGAGTGGGGATTTTAATCAGGATGATTACTCATTAAGGTTTCGAGTGTGCAACGTGAATGAGTTTATTGGCCGTATGAAGTTAGGCCATTGGCCTGTATTATCCTCTGCTGATATTTCTTTGGAATTCATTAAGTGTCCAACAGAGGATACGGGCGAGTGCTCGGTGATTTTATCAGGGAGTTTTGATGGACCGGATGAAGGTATCTCTGGTCTTGTTCACATGGCAAGTTTGAGGTTTATGACCCTAAGGCCAGTTCAGTGGGTTGTGTCGATTGATATGTCCACCATCACGGTTAGGGTGGAGATACTTAAGCGTGCGTTTGATGCTTGTCAGTCACTTCTGGACATGACTAGGCAAGTCTGGAAAAAAAGTATGAGGAATGTCATGGGTTGGCTTCATCCAGAAGTAATGACATCAGAGGCTAGGTACGGAGTCAGTAAATCAACAGAAATGGAAATTGATTTGCATACTGAAAATGGATATGCTAATTCAGACCCTAGTAAACTAGCAAGGTTTGATGTCTCTGGGTTCTATGAAGCCATTAAGCGATCAAAAGCAGATGCAATGCTTCAAGCTGACATGCCCGATTTGCTTCCTGAGTTGAAGCCCTATCAGCGCCGTGCAGCTTACTGGATGGTACATAGAGAGAAAGGATATGCAGAAAGTCCGGTCGAAAAGGGAAACAGTCAGTTCATATCACCTTTATGTTTGCCCTTGGAATTTCTTGACACATGTTCAAAAATGTTCTACAACCCATTCAGTGGAAATGTTTCGTTGCACCCAGAGAATGATTCAGCTTATGTCTGTGGTGGTATTCTTGCTGATGAGATGGGCATGGGGAAAACTGTTGAACTTCTTGCTTGTATATTTGCGCATCCGAAGTCGGCAGATGAAGACAACATATTTGCTGATTCTGAATCTCAAGCTACCGAAGATCAGAAAATCAATCTTAAAAGATTAAAAAGGGAGCGAGTTGAATGTGTATGTGGAGCTGTGAGTGAAAATATGTCATACACAGGATTATGGGTACAGTGTGACATATGCGATGCATGGCAACATGCAGACTGTGTGGGTTATTCAGCTGATGGAAAATCCATCAAATCCAATGAGGCCTCTCAAAAGCACCTGAAGAGGAAAAATACAACCACTGTAGTTGAAAGAGATGGAAAATATATTTGCCAGCTGTGCTCAGAACTGATACAGGCTACTAACTCTCCTGTGGCTACTGCTGCCACTCTTATAATTTGTCCAGCTTCTATTTTGCTCCAATGGCATTCTGAAATTATACGTCATACCCGTGCAGGTTCTTTAAAAACATGTATCTATGAAGGTGTGAGGGAGAGTTCTTTTTCAAACGCGTCGGAAATTGATATCAGTGAACTTGTTAGCGCTGACATTGTATTGACAACATATGATGTGCTTAAAGAGGACCTGTCACACGACTCTGATAGGCATGAAGGTGATCGGCGCTTGATGAGATTCCATAAAAGGTACCCTGTTGTTCCAACCGTTCTGACCAGAATATTCTGGTGGAGAGTTTGTTTGGATGAGGCTCAAATGGTGGAGAATAATGCTGCTGCGGCCACAGAAATGGCTATGCGGTTATATACTAAGCACCGTTGGTGCATTACAGGGACTCCTATTCAACGTAAACTTGATGACTTATATGGACTTCTACGATTTCTCAAAGCATGCCCATTTGATGGTTCTAGGTGGTGGGTTGAAGTTATACGAGATCCATATGAGAGGAAAGATGTGGCAGCTAGGGAATTTGCTCATAAATTTTTCAAGAAAATTATGTGGCGATCTTTGAAAGTGCATGTTGCAGATGAGTTGGAGCTTCCTCCTCAGGAGGAGTGCCTCTCTTGGCTCACTTTATCTCCAATAGAAGAACACTTTTACCAAAGGCAACACGAAACTTGTGTGAGCTATGCCCGAGAAGTCATTGACAGTCTGAAAGATGATATTCGAAAAAGAGAAGTCAGAGGATGCTTAGCATCTAATGATTCATCAGATCCACTCATCACACACGCAGAGGCAGGAAAGCTTCTGAATACACTGTTGAAGCTTCGCCAAGCCTGCTGCCACCCTCAAGTAGGAAGCTTTGGGCTTCGTTCTCTGCAACAGTCACCCATGACTATGGAGGAAATACTAATGGTTCTTGTAGGTAAAACAAAGGTAGAAGGAGAGGAAGCGCTTAGGAGGTCAGTCGTCGCTCTCAATGGGCTTGCCGGGATAGCTATACTCGAACAAAACTTCACCCAAGCTCTATCCTTGTACAAGGAAGCGCTGGATTTAGCTGAACAGCACAATGAGGATTTCCGCCTGGACCCTTTGTTGAGCATCCACATTTATCATAATCTTGCTGATATACTCCCTTTGGCAACAAACTGCTGCCCTTCAAAAGAACAGCTTCCTGGGAGCTCTAGGGAAATGGCCTCCAAGATACAGGGCATTGAAACTTGTGATCAACATGTAGCGAAGAGGCAAAAACTTTGTGGGAAAGAAGAAATTGCCAATGATGCCAGTAACTCACTGGAAGGTACATCTGAATTATCAGATAATGTTCCTGCTGCTCACCAAGAATGCGATAATGGTCCCCAGCCATCATGTAGTTCTTTCAGTGATGCATCTTTGAGAATAGCCTTTGACAATATTAAACAGAAGTACTTGTCTGTATTCAGTTCAAAGCTATCCATGGCTCAACAAGAGTTTAACAAGTCTTACACACAGGTTTGCAATGCAATCAGCGACAGAAAAGATCTAAGTACAGTTTGGTGGTTGGAAGCCCTTCTTCATGCTGAAAAGAACATAGACCTCTCTAGGGATTTGATGAGAAAGATTGAAGAGGCCCTTGTAGGAACTCTAAACAATGGGAAGTCATCGAGAATTGCCTCTCGTTTCCGAAGCATAAGTGGCCTGAAGTATCATATGCAGACTGGTTTGGACCAATTAGCAGCATCTAGGAAGACGTTGCTTGATCGACTTTTAGAAATTGATCAGACGATGGAGAAGCCAAAGGAGGAGGATATTCACCGCGTGAGAAACTGTCGAAATTGCAAGGGTTGTGATGATGGTCCTTTGTGTGTTCTCTGTGAACTAGATGAATTGTTTCGGGACTATGAAGCAAGGCTCTTTCGTAGTGAAAAAGTAGGCGGAGGGACAGCTACATGTGCTGAAGAGGCTGTGGCTTTGCAGAAGAAGAACTCTGAACTTAATCGTTTTTATCAGAATTTGTCGCAGCCAAATAAAGAGTCAACATCATCCAATTCTAATAAAGAGTCAAAGAAGAGAGATGCTGGAAAAGTTGTGGTTTCAAAAGCTCCATCTGAATTAGAGGTTGTTCTTGGAGTTATAAGAAATCATTGCAAGGCCCAATTAGGGAGGGACGGTACATCAGAAGCCACAAAGCACCTCCACGCTCTTGAGGGCATGCGCAAAGAGTATCCCCATGCAAGATCCTTGGCAATAGCCCAAGCTCAAATTCTACAAGCTTATGATGAGCTTAACATGGCAACTTCAAGATTGCGCCTAGCAGAAGATGAGGATGATAAAGCTCTTGATGCTTTAAGTGAAGAAGAATTACCTTCAGCTAGCGTCCTATATACTAGCGACAAGTTCACATCCTTAAATTCGTTGTCTGTTGTAAAAGGGAAACTTCGTTATTTAAAGGGTCTGGTACAAGCTAAACAAAAGGTGCCGTTGCAAAGTCCAAATCCTTCCTCGGTAGCTGAAGGGGCAGCCACTATGCCAACATCCACGGAACAGAAAAATGTATGCATACCTACAGGCGATAAAGAAACATGCCCGGTTTGTCAAGATCCATTAACCATTCGGAAGATGGTTTTTCCGTGTGGGCATCTTACATGCTGTAAATGTTTATTTGCAATTACTGAACGGAATAACAAGGTTCAAGATAAGTGGGTAAAATGCCCAACATGTAGACAACATACTGATGTTGGAAACATTGCTTATGCTGATGATGGGCAGAGTGAAACTAGTACTTCTTCTGTGCTTCATACAACCGAAAGTCGTGAAATGTCTGAAGCATCTGTTATGGTACAAGGTTCATATGGAACAAAGATTGAAGCAGTGACAAGAAGAATACTGTGGATAAAGTCTACAGATCCAGAGGCTAAAGTTCTTGTTTTCTCAAGCTGGCATGATGTGCTCGATGTATTGGAGCATGCCTTCGCTGCTAATGACATTACGCATGTTCGGATGAAAGGAGGAAGGAAATCACAAGTTGCCATTAGCGAATTTAAAGGCGAAAAGATAGGTGCAAAAGGAAATCACAAGATACGTGGACAGAAAGAGCAAAGATCTTTCCAAGTGTTACTGCTCCTGATTCAACATGGAGCTAACGGCCTCAATCTTCTGGAAGCGAAGCATGTTATTCTTGTCGAGCCGCTTCTCAATCCAGCAGTAGAAGCACAGGCGATCAGTCGGGTCCACCGAATCGGACAGAAAAGCAAAACCATAGCTCATCGTTTCATAGTTAAAGACACGGTTGAAGAGAGTATATATAAACTCAACAAAAGCCGGAACAGCACTGCATTCATCGGGAACACGAAGAATCAAGATCAGCCTTTCTTCACATTAAAAGACATTGAATCCTTATTCGCAACAGTGCCACCCCCGGTACCAGAAACTCGAGGAAAGGCAACTGAAGTTCCCAACGAGAACCCAACGGAAAGTCTACGGCATTTGCCACCTTCTGTGGCAGCTGCCATAGCAGCTGAGAAGAGACATAAGGAGCAGCATGCATCTTCTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0061630 GO:0061659 GO:0070013 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Helicase_C PHD SNF2_N zf-C3HC4

Protein Analysis

1679

Amino Acids

187.97

Weight (kDa)

6.21

Isoelectric Point (pI)

48.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNF2-rel_dom	PF00176	290 - 784	1.1e-64	SNF2-related domain
SHPRH_helical-1st	PF21325	800 - 884	1.4e-22	E3 ubiquitin-protein ligase SHPRH, first helical domain
SHPRH_helical-2nd	PF21324	1010 - 1133	8.4e-10	E3 ubiquitin-protein ligase SHPRH, second helical domain
Helicase_C	PF00271	1449 - 1574	8.5e-07	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0003903)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40770 AT2G40770 AT2G40770
fragaria_vesca	FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110
malus_domestica	MD01G1169300.v1.1
prunus_persica	Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1
pyrus_communis	pycom01g18200
rosa_chinensis	RchiOBHm_Chr1g0372551
rosa_laevigata	RLG00000026892
rosa_multiflora	Rmu_sc0005410.1_g000019 Rmu_sc0011709.1_g000002
rosa_roxburghii	Rroxscaffold_4G00284860
rosa_rugosa	Rorug01G0372700
rosa_samantha	Rh1AG381200 Rh1BG344600 Rh1CG358000 Rh1DG375800
rosa_wichuraiana	Rw1G033520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1499, 3614
Acc16I	TGCGCA	2 cut(s)	1131, 3695
Acc36I	ACCTGC	1 cut(s)	1550
Acc65I	GGTACC	2 cut(s)	1749, 4900
AccB1I	GGYRCC	3 cut(s)	1749, 3955, 4900
AccB7I	CCANNNNNTGG	2 cut(s)	1019, 1961
AccI	GTMKAC	3 cut(s)	4201, 4379, 4957
AccII	CGCG	2 cut(s)	1616, 3297
AciI	CCGC	6 cut(s)	1838, 1858, 2581, 3295, 3414, 4667
AclWI	GGATC	6 cut(s)	27, 1988, 2267, 3711, 4073, 4379
AcoI	YGGCCR	2 cut(s)	325, 1839
AcuI	CTGAAG	8 cut(s)	225, 1406, 3200, 3456, 3831, 4011, 4323, 4947
AdeI	CACNNNGTG	1 cut(s)	3298
AfeI	AGCGCT	3 cut(s)	1648, 2454, 2547
AfiI	CCNNNNNNNGG	6 cut(s)	455, 1019, 1961, 2354, 3082, 3639
AflII	CTTAAG	1 cut(s)	566
AflIII	ACRYGT	6 cut(s)	989, 1574, 1614, 2731, 3428, 4196
AhlI	ACTAGT	1 cut(s)	4256
AjnI	CCWGG	2 cut(s)	2583, 2674
AjuI	GAANNNNNNNTTGG	2 cut(s)	3726, 3758
Alw21I	GWGCWC	5 cut(s)	192, 419, 1448, 2684, 4431
Alw26I	GTCTC	2 cut(s)	784, 4999
AlwI	GGATC	6 cut(s)	27, 1988, 2267, 3711, 4073, 4379
Ama87I	CYCGRG	2 cut(s)	2193, 4911
Aor51HI	AGCGCT	3 cut(s)	1648, 2454, 2547
ArsI	GACNNNNNNTTYG	4 cut(s)	1145, 1177, 3497, 3529
Asp700I	GAANNNNTTC	4 cut(s)	1027, 2670, 3048, 3369
Asp718I	GGTACC	2 cut(s)	1749, 4900
AspLEI	GCGC	8 cut(s)	876, 1132, 1649, 1728, 2455, 2548, 3696, 3789
AsuC2I	CCSGG	3 cut(s)	2491, 4067, 4898
AsuHPI	GGTGA	8 cut(s)	260, 433, 948, 1730, 2379, 2509, 2828, 3284
AsuII	TTCGAA	1 cut(s)	2227
AsuNHI	GCTAGC	1 cut(s)	3851
AvaI	CYCGRG	2 cut(s)	2193, 4911
AvaII	GGWCC	7 cut(s)	446, 1687, 2587, 2855, 3205, 3335, 4708
AxyI	CCTNAGG	2 cut(s)	503, 2106
BanI	GGYRCC	3 cut(s)	1749, 3955, 4900
BanII	GRGCYC	1 cut(s)	2684
BarI	GAAGNNNNNNTAC	2 cut(s)	3825, 3857
BbsI	GAAGAC	2 cut(s)	1158, 3233
Bbv12I	GWGCWC	5 cut(s)	192, 419, 1448, 2684, 4431
BceAI	ACGGC	6 cut(s)	130, 312, 861, 3943, 4639, 4976
BciT130I	CCWGG	2 cut(s)	2585, 2676
BciVI	GTATCC	2 cut(s)	397, 3714
BclI	TGATCA	3 cut(s)	70, 2725, 3256
BcnI	CCSGG	3 cut(s)	2491, 4067, 4898
BcoDI	GTCTC	2 cut(s)	784, 4999
BcuI	ACTAGT	1 cut(s)	4256
BfmI	CTRYAG	3 cut(s)	1407, 4044, 4380
BfoI	RGCGCY	5 cut(s)	877, 1650, 1729, 2456, 2549
BfrI	CTTAAG	1 cut(s)	566
BfuAI	ACCTGC	1 cut(s)	1550
BfuI	GTATCC	2 cut(s)	397, 3714
BglII	AGATCT	2 cut(s)	3019, 4580
BlpI	GCTNAGC	1 cut(s)	2251
BmcAI	AGTACT	2 cut(s)	2927, 4261
Bme1390I	CCNGG	5 cut(s)	2491, 2585, 2676, 4067, 4898
Bme18I	GGWCC	7 cut(s)	446, 1687, 2587, 2855, 3205, 3335, 4708
BmeT110I	CYCGRG	2 cut(s)	2193, 4911
BmiI	GGNNCC	7 cut(s)	26, 1751, 2589, 2857, 3957, 4709, 4902
BmrFI	CCNGG	5 cut(s)	2491, 2585, 2676, 4067, 4898
BmtI	GCTAGC	1 cut(s)	3855
BpiI	GAAGAC	2 cut(s)	1158, 3233
BplI	GAGNNNNNCTC	2 cut(s)	2105, 2137
Bpu10I	CCTNAGC	1 cut(s)	2456
Bpu1102I	GCTNAGC	1 cut(s)	2251
Bpu14I	TTCGAA	1 cut(s)	2227
BpuEI	CTTGAG	4 cut(s)	1164, 2334, 3704, 4396
BpuMI	CCSGG	3 cut(s)	2491, 4067, 4898
BsaAI	YACGTR	1 cut(s)	4562
BsaBI	GATNNNNATC	1 cut(s)	270
BsaJI	CCNNGG	7 cut(s)	972, 2675, 2955, 3720, 3981, 4017, 4896
BsaWI	WCCGGW	3 cut(s)	35, 448, 925
Bsc4I	CCNNNNNNNGG	6 cut(s)	455, 1019, 1961, 2354, 3082, 3639
Bse1I	ACTGG	6 cut(s)	132, 509, 893, 2784, 2799, 3202
Bse21I	CCTNAGG	2 cut(s)	503, 2106
Bse3DI	GCAATG	5 cut(s)	70, 828, 3004, 3622, 4224
Bse8I	GATNNNNATC	1 cut(s)	270
BseBI	CCWGG	2 cut(s)	2585, 2676
BseDI	CCNNGG	7 cut(s)	972, 2675, 2955, 3720, 3981, 4017, 4896
BseJI	GATNNNNATC	1 cut(s)	270
BseLI	CCNNNNNNNGG	6 cut(s)	455, 1019, 1961, 2354, 3082, 3639
BseMI	GCAATG	5 cut(s)	70, 828, 3004, 3622, 4224
BseMII	CTCAG	4 cut(s)	846, 1461, 2120, 4989
BseNI	ACTGG	6 cut(s)	132, 509, 893, 2784, 2799, 3202
BseRI	GAGGAG	3 cut(s)	2094, 2126, 3296
BseYI	CCCAGC	1 cut(s)	2859
BsgI	GTGCAG	2 cut(s)	900, 1578
Bsh1236I	CGCG	2 cut(s)	1616, 3297
Bsh1285I	CGRYCG	1 cut(s)	930
BshNI	GGYRCC	3 cut(s)	1749, 3955, 4900
BsiEI	CGRYCG	1 cut(s)	930
BsiHKAI	GWGCWC	5 cut(s)	192, 419, 1448, 2684, 4431
BsiHKCI	CYCGRG	2 cut(s)	2193, 4911
BsiSI	CCGG	7 cut(s)	36, 449, 926, 2490, 4067, 4801, 4898
BslFI	GGGAC	5 cut(s)	1906, 2841, 3389, 3433, 3659
BslI	CCNNNNNNNGG	6 cut(s)	455, 1019, 1961, 2354, 3082, 3639
BsmAI	GTCTC	2 cut(s)	784, 4999
BsmFI	GGGAC	5 cut(s)	1906, 2841, 3389, 3433, 3659
BsmI	GAATGC	3 cut(s)	1531, 2849, 4814
BsoBI	CYCGRG	2 cut(s)	2193, 4911
Bsp119I	TTCGAA	1 cut(s)	2227
Bsp1286I	GDGCHC	5 cut(s)	192, 419, 1448, 2684, 4431
Bsp1407I	TGTACA	1 cut(s)	2535
Bsp1720I	GCTNAGC	1 cut(s)	2251
Bsp19I	CCATGG	1 cut(s)	2955
BspACI	CCGC	6 cut(s)	1838, 1858, 2581, 3295, 3414, 4667
BspCNI	CTCAG	4 cut(s)	847, 1460, 2119, 4990
BspFNI	CGCG	2 cut(s)	1616, 3297
BspLI	GGNNCC	7 cut(s)	26, 1751, 2589, 2857, 3957, 4709, 4902
BspMI	ACCTGC	1 cut(s)	1550
BspOI	GCTAGC	1 cut(s)	3855
BspPI	GGATC	6 cut(s)	27, 1988, 2267, 3711, 4073, 4379
BspT104I	TTCGAA	1 cut(s)	2227
BspT107I	GGYRCC	3 cut(s)	1749, 3955, 4900
BspTI	CTTAAG	1 cut(s)	566
BsrDI	GCAATG	5 cut(s)	70, 828, 3004, 3622, 4224
BsrGI	TGTACA	1 cut(s)	2535
BsrI	ACTGG	6 cut(s)	132, 509, 893, 2784, 2799, 3202
BssECI	CCNNGG	7 cut(s)	972, 2675, 2955, 3720, 3981, 4017, 4896
BssT1I	CCWWGG	3 cut(s)	972, 2955, 3720
Bst2UI	CCWGG	2 cut(s)	2585, 2676
Bst6I	CTCTTC	7 cut(s)	1382, 2735, 3099, 3432, 3542, 4767, 4997
BstAFI	CTTAAG	1 cut(s)	566
BstAPI	GCANNNNNTGC	1 cut(s)	4426
BstAUI	TGTACA	1 cut(s)	2535
BstBAI	YACGTR	1 cut(s)	4562
BstBI	TTCGAA	1 cut(s)	2227
BstDEI	CTNAG	9 cut(s)	503, 855, 1447, 1869, 2106, 2251, 2456, 3023, 4998
BstDSI	CCRYGG	2 cut(s)	2955, 4017
BstENI	CCTNNNNNAGG	1 cut(s)	2352
BstFNI	CGCG	2 cut(s)	1616, 3297
BstH2I	RGCGCY	5 cut(s)	877, 1650, 1729, 2456, 2549
BstHHI	GCGC	8 cut(s)	876, 1132, 1649, 1728, 2455, 2548, 3696, 3789
BstMAI	GTCTC	2 cut(s)	784, 4999
BstMCI	CGRYCG	1 cut(s)	930
BstNI	CCWGG	2 cut(s)	2585, 2676
BstSCI	CCNGG	5 cut(s)	2489, 2583, 2674, 4065, 4896
BstSFI	CTRYAG	3 cut(s)	1407, 4044, 4380
BstUI	CGCG	2 cut(s)	1616, 3297
BstV2I	GAAGAC	2 cut(s)	1158, 3233
BstX2I	RGATCY	7 cut(s)	1993, 2272, 3019, 3716, 4078, 4384, 4580
BstXI	CCANNNNNNTGG	3 cut(s)	516, 1849, 2398
BstYI	RGATCY	7 cut(s)	1993, 2272, 3019, 3716, 4078, 4384, 4580
Bsu36I	CCTNAGG	2 cut(s)	503, 2106
BsuI	GTATCC	2 cut(s)	397, 3714
BtgI	CCRYGG	2 cut(s)	2955, 4017
BtgZI	GCGATG	1 cut(s)	1317
BtsI	GCAGTG	2 cut(s)	4356, 4809
BveI	ACCTGC	1 cut(s)	1550
CfoI	GCGC	8 cut(s)	876, 1132, 1649, 1728, 2455, 2548, 3696, 3789
CseI	GACGC	2 cut(s)	1605, 3844
CspCI	CAANNNNNGTGG	6 cut(s)	1393, 1428, 4160, 4195, 4543, 4578
DdeI	CTNAG	9 cut(s)	503, 855, 1447, 1869, 2106, 2251, 2456, 3023, 4998
DraI	TTTAAA	3 cut(s)	1569, 3927, 4522
DraIII	CACNNNGTG	1 cut(s)	3298
EaeI	YGGCCR	2 cut(s)	325, 1839
Eam1104I	CTCTTC	7 cut(s)	1382, 2735, 3099, 3432, 3542, 4767, 4997
EarI	CTCTTC	7 cut(s)	1382, 2735, 3099, 3432, 3542, 4767, 4997
EciI	GGCGGA	2 cut(s)	2570, 3429
Ecl136II	GAGCTC	1 cut(s)	2682
Eco130I	CCWWGG	3 cut(s)	972, 2955, 3720
Eco147I	AGGCCT	1 cut(s)	1370
Eco24I	GRGCYC	1 cut(s)	2684
Eco32I	GATATC	1 cut(s)	1630
Eco47I	GGWCC	7 cut(s)	446, 1687, 2587, 2855, 3205, 3335, 4708
Eco47III	AGCGCT	3 cut(s)	1648, 2454, 2547
Eco53kI	GAGCTC	1 cut(s)	2682
Eco57I	CTGAAG	8 cut(s)	225, 1406, 3200, 3456, 3831, 4011, 4323, 4947
Eco81I	CCTNAGG	2 cut(s)	503, 2106
Eco88I	CYCGRG	2 cut(s)	2193, 4911
EcoICRI	GAGCTC	1 cut(s)	2682
EcoNI	CCTNNNNNAGG	1 cut(s)	2352
EcoO109I	RGGNCCY	3 cut(s)	24, 1687, 3109
EcoRI	GAATTC	1 cut(s)	380
EcoRII	CCWGG	2 cut(s)	2583, 2674
EcoRV	GATATC	1 cut(s)	1630
EcoT14I	CCWWGG	3 cut(s)	972, 2955, 3720
EcoT22I	ATGCAT	4 cut(s)	1309, 2890, 4040, 5026
EcoT38I	GRGCYC	1 cut(s)	2684
ErhI	CCWWGG	3 cut(s)	972, 2955, 3720
FalI	AAGNNNNNCTT	8 cut(s)	2655, 2687, 3036, 3068, 3096, 3128, 3900, 3932
FaqI	GGGAC	5 cut(s)	1906, 2841, 3389, 3433, 3659
FauNDI	CATATG	5 cut(s)	1298, 1669, 1999, 3189, 4330
FbaI	TGATCA	3 cut(s)	70, 2725, 3256
FblI	GTMKAC	3 cut(s)	4201, 4379, 4957
FriOI	GRGCYC	1 cut(s)	2684
FspI	TGCGCA	2 cut(s)	1131, 3695
GlaI	GCGC	8 cut(s)	875, 1131, 1648, 1727, 2454, 2547, 3695, 3788
GsaI	CCCAGC	1 cut(s)	2863
HaeII	RGCGCY	5 cut(s)	877, 1650, 1729, 2456, 2549
HapII	CCGG	7 cut(s)	36, 449, 926, 2490, 4067, 4801, 4898
HgaI	GACGC	2 cut(s)	1605, 3844
HhaI	GCGC	8 cut(s)	876, 1132, 1649, 1728, 2455, 2548, 3696, 3789
Hin6I	GCGC	8 cut(s)	874, 1130, 1647, 1726, 2453, 2546, 3694, 3787
HinP1I	GCGC	8 cut(s)	874, 1130, 1647, 1726, 2453, 2546, 3694, 3787
HincII	GTYRAC	1 cut(s)	3516
HindII	GTYRAC	1 cut(s)	3516
HindIII	AAGCTT	4 cut(s)	2303, 2324, 2359, 3750
HpaII	CCGG	7 cut(s)	36, 449, 926, 2490, 4067, 4801, 4898
HphI	GGTGA	8 cut(s)	260, 433, 948, 1730, 2379, 2509, 2828, 3284
Hpy99I	CGWCG	2 cut(s)	1621, 2474
HpyCH4IV	ACGT	5 cut(s)	309, 1546, 1906, 3230, 4561
HpyF3I	CTNAG	9 cut(s)	503, 855, 1447, 1869, 2106, 2251, 2456, 3023, 4998
HpySE526I	ACGT	5 cut(s)	309, 1546, 1906, 3230, 4561
HspAI	GCGC	8 cut(s)	874, 1130, 1647, 1726, 2453, 2546, 3694, 3787
KpnI	GGTACC	2 cut(s)	1753, 4904
Ksp22I	TGATCA	3 cut(s)	70, 2725, 3256
MaeII	ACGT	5 cut(s)	309, 1546, 1906, 3230, 4561
MaeIII	GTNAC	7 cut(s)	591, 1292, 1692, 2385, 2786, 4351, 4593
MflI	RGATCY	7 cut(s)	1993, 2272, 3019, 3716, 4078, 4384, 4580
MhlI	GDGCHC	5 cut(s)	192, 419, 1448, 2684, 4431
MluI	ACGCGT	1 cut(s)	1614
MlyI	GAGTC	6 cut(s)	64, 699, 1694, 1888, 3521, 3548
MmeI	TCCRAC	6 cut(s)	419, 1599, 1787, 2073, 3021, 4198
Mph1103I	ATGCAT	4 cut(s)	1309, 2890, 4040, 5026
MroXI	GAANNNNTTC	4 cut(s)	1027, 2670, 3048, 3369
MslI	CAYNNNNRTG	6 cut(s)	961, 1554, 1716, 1847, 2396, 4212
MspA1I	CMGCKG	4 cut(s)	1340, 1441, 4985, 4997
MspCI	CTTAAG	1 cut(s)	566
MspI	CCGG	7 cut(s)	36, 449, 926, 2490, 4067, 4801, 4898
MspR9I	CCNGG	5 cut(s)	2491, 2585, 2676, 4067, 4898
Mva1269I	GAATGC	3 cut(s)	1531, 2849, 4814
MvaI	CCWGG	2 cut(s)	2585, 2676
MvnI	CGCG	2 cut(s)	1616, 3297
NciI	CCSGG	3 cut(s)	2491, 4067, 4898
NcoI	CCATGG	1 cut(s)	2955
NdeI	CATATG	5 cut(s)	1298, 1669, 1999, 3189, 4330
NheI	GCTAGC	1 cut(s)	3851
NlaIV	GGNNCC	7 cut(s)	26, 1751, 2589, 2857, 3957, 4709, 4902
NmuCI	GTSAC	5 cut(s)	591, 1292, 1692, 2385, 4351
NsbI	TGCGCA	2 cut(s)	1131, 3695
NsiI	ATGCAT	4 cut(s)	1309, 2890, 4040, 5026
NspV	TTCGAA	1 cut(s)	2227
PaeI	GCATGC	4 cut(s)	1953, 3694, 4448, 5024
PaeR7I	CTCGAG	1 cut(s)	4911
PceI	AGGCCT	1 cut(s)	1370
PciI	ACATGT	5 cut(s)	989, 1574, 2731, 3428, 4196
PcsI	WCGNNNNNNNCGW	2 cut(s)	1552, 4711
PctI	GAATGC	3 cut(s)	1531, 2849, 4814
PdmI	GAANNNNTTC	4 cut(s)	1027, 2670, 3048, 3369
PflMI	CCANNNNNTGG	2 cut(s)	1019, 1961
PleI	GAGTC	6 cut(s)	63, 698, 1694, 1888, 3520, 3547
PpsI	GAGTC	6 cut(s)	63, 698, 1694, 1888, 3520, 3547
Ppu21I	YACGTR	1 cut(s)	4562
PpuMI	RGGWCCY	1 cut(s)	1687
PscI	ACATGT	5 cut(s)	989, 1574, 2731, 3428, 4196
PsiI	TTATAA	2 cut(s)	1499, 3614
Psp124BI	GAGCTC	1 cut(s)	2684
Psp5II	RGGWCCY	1 cut(s)	1687
Psp6I	CCWGG	2 cut(s)	2583, 2674
PspFI	CCCAGC	1 cut(s)	2859
PspGI	CCWGG	2 cut(s)	2583, 2674
PspN4I	GGNNCC	7 cut(s)	26, 1751, 2589, 2857, 3957, 4709, 4902
PspPPI	RGGWCCY	1 cut(s)	1687
PspXI	VCTCGAGB	1 cut(s)	4911
PsuI	RGATCY	7 cut(s)	1993, 2272, 3019, 3716, 4078, 4384, 4580
PvuII	CAGCTG	4 cut(s)	1340, 1441, 4985, 4997
RseI	CAYNNNNRTG	6 cut(s)	961, 1554, 1716, 1847, 2396, 4212
SacI	GAGCTC	1 cut(s)	2684
ScaI	AGTACT	2 cut(s)	2927, 4261
SchI	GAGTC	6 cut(s)	64, 699, 1694, 1888, 3521, 3548
ScrFI	CCNGG	5 cut(s)	2491, 2585, 2676, 4067, 4898
SduI	GDGCHC	5 cut(s)	192, 419, 1448, 2684, 4431
SfcI	CTRYAG	3 cut(s)	1407, 4044, 4380
Sfr274I	CTCGAG	1 cut(s)	4911
SfuI	TTCGAA	1 cut(s)	2227
SinI	GGWCC	7 cut(s)	446, 1687, 2587, 2855, 3205, 3335, 4708
SlaI	CTCGAG	1 cut(s)	4911
SmiMI	CAYNNNNRTG	6 cut(s)	961, 1554, 1716, 1847, 2396, 4212
SmlI	CTYRAG	6 cut(s)	566, 1179, 2349, 3683, 4411, 4911
SmoI	CTYRAG	6 cut(s)	566, 1179, 2349, 3683, 4411, 4911
SpeI	ACTAGT	1 cut(s)	4256
SphI	GCATGC	4 cut(s)	1953, 3694, 4448, 5024
SseBI	AGGCCT	1 cut(s)	1370
SsiI	CCGC	6 cut(s)	1838, 1858, 2581, 3295, 3414, 4667
SspI	AATATT	2 cut(s)	1782, 2914
SstI	GAGCTC	1 cut(s)	2684
StuI	AGGCCT	1 cut(s)	1370
StyD4I	CCNGG	5 cut(s)	2489, 2583, 2674, 4065, 4896
StyI	CCWWGG	3 cut(s)	972, 2955, 3720
TaiI	ACGT	5 cut(s)	312, 1549, 1909, 3233, 4564
TaqII	GACCGA	1 cut(s)	156
TatI	WGTACW	4 cut(s)	2535, 2925, 3026, 4259
TauI	GCSGC	2 cut(s)	1841, 4669
TseFI	GTSAC	5 cut(s)	591, 1292, 1692, 2385, 4351
Tsp45I	GTSAC	5 cut(s)	591, 1292, 1692, 2385, 4351
TspGWI	ACGGA	5 cut(s)	702, 4034, 4098, 4171, 4964
Van91I	CCANNNNNTGG	2 cut(s)	1019, 1961
Vha464I	CTTAAG	1 cut(s)	566
VpaK11BI	GGWCC	7 cut(s)	446, 1687, 2587, 2855, 3205, 3335, 4708
XagI	CCTNNNNNAGG	1 cut(s)	2352
XcmI	CCANNNNNNNNNTGG	3 cut(s)	35, 2791, 4096
XhoI	CTCGAG	1 cut(s)	4911
XmiI	GTMKAC	3 cut(s)	4201, 4379, 4957
XmnI	GAANNNNTTC	4 cut(s)	1027, 2670, 3048, 3369
ZrmI	AGTACT	2 cut(s)	2927, 4261
Zsp2I	ATGCAT	4 cut(s)	1309, 2890, 4040, 5026

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.