AT2G42980

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013789
Physical Location & Seq
Reverse (-)
17875005 .. 17876779
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G42980.1

Sequence Viewer

Length: 1584 bp
ATGTCGACCAAACTCAGTATATTTCTTCTTGGCCTGATTTTGTTCTCTGTCTCACCATTTTCCGGAGACTGTAGAACTCTTTCAGGGAAACATGAGCATTACTCTTCATCCCTTAACATGTTTAATTCCCAAGATACGATGCGTTTCAGTTCTGCTTCTTCTTCAACAAGCAACGATTGCGGTTTCTCCTCCAAGGAACATGATCCTTCCAAGGAACATACCAGAGAATCGGTGAAGCCTCAATCAAGGATTAAACAAGAAACAAAGAGAACAACACATTCAGTTGTTGATCTTCAAATCCAAGACCTCACAAGAATTAAGACGCTTCATGCTCGGTTCAACAAATCGAAGAAACAGAAAAACGAGAAGGTTAGGAAGAAGATTACCTCAGATATTTCTTTGGTGGGAGCTCCAGAGGTTTCACCGGGGAAGCTGATAGCAACACTTGAGTCTGGTATGACGCTCGGTTCTGGTGAATACTTCATGGACGTGCTTGTGGGCACACCACCAAAACACTTCTCATTGATTCTTGACACAGGGAGTGACTTAAACTGGCTCCAATGCCTTCCTTGCTACGACTGTTTCCATCAAAACGGTATGTTTTATGATCCAAAAACGTCCGCTTCATTCAAGAACATAACATGCAATGACCCTAGATGCAGCCTTATCTCATCACCCGATCCTCCAGTACAGTGCGAATCAGATAACCAATCGTGCCCTTATTTCTACTGGTATGGAGACAGGTCGAACACAACTGGAGATTTCGCGGTTGAGACTTTCACCGTTAATCTCACGACCACTGAGGGAGGAAGCTCAGAGTACAAGGTGGGAAATATGATGTTTGGGTGTGGCCATTGGAACCGAGGTCTCTTCAGTGGTGCTTCAGGTCTGCTTGGGTTAGGAAGAGGACCTCTTTCGTTTTCTTCTCAGCTTCAGTCTCTCTATGGCCATTCCTTTTCCTACTGTCTTGTCGATAGAAACAGCAACACCAATGTAAGCAGCAAGTTGATATTTGGAGAAGACAAAGATTTGCTGAACCACACAAATCTGAACTTCACTTCTTTTGTGAACGGGAAGGAGAATTCAGTGGAGACATTTTACTATATACAGATCAAATCCATTCTAGTCGGCGGCAAAGCTCTAGACATACCTGAAGAAACATGGAATATCTCATCAGATGGTGATGGTGGAACCATCATTGATTCTGGTACAACCTTAAGCTATTTCGCAGAACCTGCATATGAGATCATCAAAAACAAGTTTGCAGAGAAAATGAAAGAAAACTACCCAATATTTAGAGATTTCCCGGTCCTTGATCCTTGCTTCAATGTATCTGGCATAGAGGAGAACAACATCCACCTACCTGAGCTCGGGATTGCATTTGTAGACGGCACAGTTTGGAATTTCCCTGCTGAGAACTCCTTCATTTGGTTGAGCGAGGATTTGGTTTGCTTGGCAATTCTAGGAACTCCAAAATCTACCTTCTCGATCATCGGAAACTACCAGCAACAGAATTTCCATATACTCTATGATACAAAGAGGTCTAGGCTAGGGTTCACACCCACAAAGTGTGCGGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18A TAXi_C TAXi_N

Protein Analysis

527

Amino Acids

58.65

Weight (kDa)

5.84

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 159 - 212 5.1e-07 Eukaryotic aspartyl protease
TAXi_N PF14543 160 - 339 6.9e-51 Xylanase inhibitor N-terminal
Asp PF00026 294 - 521 2.7e-07 Eukaryotic aspartyl protease
TAXi_C PF14541 367 - 520 4e-36 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42980
malus_domestica MD01G1081400.v1.1 MD01G1081500.v1.1 MD01G1081600.v1.1 MD01G1081900.v1.1
prunus_persica Prupe.2G189100_v2.0.a1
pyrus_communis pycom07g14880
rosa_chinensis RchiOBHm_Chr1g0359581
rosa_laevigata RLG00000027860
rosa_multiflora Rmu_sc0002556.1_g000022
rosa_roxburghii Rroxscaffold_4G00296910
rosa_samantha Rh1AG285600 Rh1BG251700 Rh1BG251800 Rh1DG280900 Rh1DG281000
rosa_wichuraiana Rw1G025390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1243
AccI GTMKAC 2 cut(s) 5, 1386
AccII CGCG 1 cut(s) 767
AccIII TCCGGA 1 cut(s) 62
AciI CCGC 5 cut(s) 180, 621, 767, 1131, 1574
AclWI GGATC 4 cut(s) 197, 602, 674, 1310
AcoI YGGCCR 2 cut(s) 850, 946
AcsI RAATTY 3 cut(s) 1081, 1402, 1513
AcuI CTGAAG 4 cut(s) 856, 867, 917, 1173
AdeI CACNNNGTG 1 cut(s) 1569
AfaI GTAC 3 cut(s) 690, 821, 1210
AfiI CCNNNNNNNGG 3 cut(s) 62, 1370, 1428
AflII CTTAAG 1 cut(s) 1216
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 5 cut(s) 165, 296, 340, 631, 1327
AjiI CACGTC 1 cut(s) 490
AluBI AGCT 7 cut(s) 410, 433, 813, 931, 1139, 1221, 1369
AluI AGCT 7 cut(s) 410, 433, 813, 931, 1139, 1221, 1369
Alw21I GWGCWC 2 cut(s) 412, 1371
Alw26I GTCTC 7 cut(s) 55, 60, 732, 767, 872, 942, 1085
AlwI GGATC 4 cut(s) 197, 602, 674, 1310
AlwNI CAGNNNCTG 1 cut(s) 1235
Ama87I CYCGRG 1 cut(s) 1370
Aor13HI TCCGGA 1 cut(s) 62
AoxI GGCC 3 cut(s) 31, 850, 946
ApeKI GCWGC 2 cut(s) 660, 999
ApoI RAATTY 3 cut(s) 1081, 1402, 1513
Asp700I GAANNNNTTC 2 cut(s) 79, 1421
AspS9I GGNCC 2 cut(s) 908, 1309
AsuC2I CCSGG 2 cut(s) 426, 1307
AsuHPI GGTGA 7 cut(s) 45, 244, 414, 485, 666, 772, 1193
AvaI CYCGRG 1 cut(s) 1370
AvaII GGWCC 2 cut(s) 908, 1309
BaeGI GKGCMC 2 cut(s) 503, 719
BalI TGGCCA 2 cut(s) 852, 948
BanII GRGCYC 2 cut(s) 412, 1371
BbsI GAAGAC 1 cut(s) 1026
Bbv12I GWGCWC 2 cut(s) 412, 1371
BbvI GCAGC 2 cut(s) 672, 1011
BccI CCATC 4 cut(s) 594, 1172, 1178, 1202
BceAI ACGGC 1 cut(s) 1405
BcnI CCSGG 2 cut(s) 426, 1307
BcoDI GTCTC 7 cut(s) 55, 60, 732, 767, 872, 942, 1085
BfaI CTAG 6 cut(s) 654, 1124, 1142, 1463, 1545, 1550
BfmI CTRYAG 1 cut(s) 70
BfrI CTTAAG 1 cut(s) 1216
BfuAI ACCTGC 1 cut(s) 1243
BisI GCNGC 3 cut(s) 661, 1000, 1132
BlsI GCNGC 3 cut(s) 662, 1001, 1133
Bme1390I CCNGG 2 cut(s) 426, 1307
Bme18I GGWCC 2 cut(s) 908, 1309
BmeT110I CYCGRG 1 cut(s) 1370
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 908, 1309
BmiI GGNNCC 3 cut(s) 557, 860, 1192
BmrFI CCNGG 2 cut(s) 426, 1307
BmsI GCATC 2 cut(s) 129, 647
BpiI GAAGAC 1 cut(s) 1026
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BpmI CTGGAG 3 cut(s) 396, 669, 777
Bpu10I CCTNAGC 1 cut(s) 1365
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 2 cut(s) 426, 1307
BsaI GGTCTC 1 cut(s) 872
BsaJI CCNNGG 4 cut(s) 192, 210, 425, 862
BsaWI WCCGGW 1 cut(s) 62
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 1370, 1428
Bse1I ACTGG 4 cut(s) 557, 686, 734, 760
Bse3DI GCAATG 1 cut(s) 652
BseAI TCCGGA 1 cut(s) 62
BseDI CCNNGG 4 cut(s) 192, 210, 425, 862
BseGI GGATG 2 cut(s) 107, 1353
BseLI CCNNNNNNNGG 3 cut(s) 62, 1370, 1428
BseMI GCAATG 1 cut(s) 652
BseMII CTCAG 7 cut(s) 28, 402, 792, 828, 941, 1356, 1404
BseNI ACTGG 4 cut(s) 557, 686, 734, 760
BseRI GAGGAG 2 cut(s) 178, 1358
BseSI GKGCMC 2 cut(s) 503, 719
BseXI GCAGC 2 cut(s) 672, 1011
Bsh1236I CGCG 1 cut(s) 767
BshFI GGCC 3 cut(s) 33, 852, 948
BsiHKAI GWGCWC 2 cut(s) 412, 1371
BsiHKCI CYCGRG 1 cut(s) 1370
BsiSI CCGG 3 cut(s) 63, 425, 1307
BslI CCNNNNNNNGG 3 cut(s) 62, 1370, 1428
BsmAI GTCTC 7 cut(s) 55, 60, 732, 767, 872, 942, 1085
BsnI GGCC 3 cut(s) 33, 852, 948
Bso31I GGTCTC 1 cut(s) 872
BsoBI CYCGRG 1 cut(s) 1370
Bsp1286I GDGCHC 4 cut(s) 412, 503, 719, 1371
Bsp13I TCCGGA 1 cut(s) 62
Bsp143I GATC 8 cut(s) 202, 289, 607, 679, 1110, 1245, 1315, 1488
BspACI CCGC 5 cut(s) 180, 621, 767, 1131, 1574
BspANI GGCC 3 cut(s) 33, 852, 948
BspCNI CTCAG 7 cut(s) 27, 401, 793, 827, 940, 1357, 1405
BspEI TCCGGA 1 cut(s) 62
BspFNI CGCG 1 cut(s) 767
BspLI GGNNCC 3 cut(s) 557, 860, 1192
BspMI ACCTGC 1 cut(s) 1243
BspPI GGATC 4 cut(s) 197, 602, 674, 1310
BspTI CTTAAG 1 cut(s) 1216
BspTNI GGTCTC 1 cut(s) 872
BsrDI GCAATG 1 cut(s) 652
BsrI ACTGG 4 cut(s) 557, 686, 734, 760
BssECI CCNNGG 4 cut(s) 192, 210, 425, 862
BssMI GATC 8 cut(s) 202, 289, 607, 679, 1110, 1245, 1315, 1488
BssT1I CCWWGG 2 cut(s) 192, 210
Bst4CI ACNGT 7 cut(s) 71, 581, 596, 693, 784, 965, 1396
Bst6I CTCTTC 3 cut(s) 109, 875, 898
BstAFI CTTAAG 1 cut(s) 1216
BstAPI GCANNNNNTGC 2 cut(s) 177, 1235
BstDEI CTNAG 7 cut(s) 14, 388, 801, 814, 927, 1365, 1413
BstF5I GGATG 2 cut(s) 107, 1353
BstFNI CGCG 1 cut(s) 767
BstKTI GATC 8 cut(s) 205, 292, 610, 682, 1113, 1248, 1318, 1491
BstMAI GTCTC 7 cut(s) 55, 60, 732, 767, 872, 942, 1085
BstMBI GATC 8 cut(s) 202, 289, 607, 679, 1110, 1245, 1315, 1488
BstMWI GCNNNNNNNGC 3 cut(s) 177, 570, 1235
BstNSI RCATGY 2 cut(s) 121, 645
BstSCI CCNGG 2 cut(s) 424, 1305
BstSFI CTRYAG 1 cut(s) 70
BstSLI GKGCMC 2 cut(s) 503, 719
BstUI CGCG 1 cut(s) 767
BstV1I GCAGC 2 cut(s) 672, 1011
BstV2I GAAGAC 1 cut(s) 1026
BsuRI GGCC 3 cut(s) 33, 852, 948
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 2 cut(s) 107, 1353
BtsIMutI CAGTG 4 cut(s) 698, 798, 880, 1092
BveI ACCTGC 1 cut(s) 1243
CaiI CAGNNNCTG 1 cut(s) 1235
Cfr13I GGNCC 2 cut(s) 908, 1309
CseI GACGC 2 cut(s) 331, 469
Csp6I GTAC 3 cut(s) 689, 820, 1209
CviAII CATG 7 cut(s) 92, 118, 200, 329, 484, 642, 1161
CviQI GTAC 3 cut(s) 689, 820, 1209
DdeI CTNAG 7 cut(s) 14, 388, 801, 814, 927, 1365, 1413
DpnI GATC 8 cut(s) 204, 291, 609, 681, 1112, 1247, 1317, 1490
DpnII GATC 8 cut(s) 202, 289, 607, 679, 1110, 1245, 1315, 1488
DraIII CACNNNGTG 1 cut(s) 1569
EaeI YGGCCR 2 cut(s) 850, 946
Eam1104I CTCTTC 3 cut(s) 109, 875, 898
EarI CTCTTC 3 cut(s) 109, 875, 898
Ecl136II GAGCTC 2 cut(s) 410, 1369
Eco130I CCWWGG 2 cut(s) 192, 210
Eco24I GRGCYC 2 cut(s) 412, 1371
Eco31I GGTCTC 1 cut(s) 872
Eco47I GGWCC 2 cut(s) 908, 1309
Eco53kI GAGCTC 2 cut(s) 410, 1369
Eco57I CTGAAG 4 cut(s) 856, 867, 917, 1173
Eco88I CYCGRG 1 cut(s) 1370
EcoICRI GAGCTC 2 cut(s) 410, 1369
EcoO109I RGGNCCY 1 cut(s) 908
EcoRI GAATTC 1 cut(s) 1081
EcoT14I CCWWGG 2 cut(s) 192, 210
EcoT38I GRGCYC 2 cut(s) 412, 1371
ErhI CCWWGG 2 cut(s) 192, 210
FaeI CATG 7 cut(s) 95, 121, 203, 332, 487, 645, 1164
FatI CATG 7 cut(s) 91, 117, 199, 328, 483, 641, 1160
FauNDI CATATG 1 cut(s) 1240
FblI GTMKAC 2 cut(s) 5, 1386
Fnu4HI GCNGC 3 cut(s) 661, 1000, 1132
FokI GGATG 2 cut(s) 94, 1340
FriOI GRGCYC 2 cut(s) 412, 1371
Fsp4HI GCNGC 3 cut(s) 661, 1000, 1132
FspBI CTAG 6 cut(s) 654, 1124, 1142, 1463, 1545, 1550
GluI GCNGC 3 cut(s) 661, 1000, 1132
GsuI CTGGAG 3 cut(s) 396, 669, 777
HaeIII GGCC 3 cut(s) 33, 852, 948
HapII CCGG 3 cut(s) 63, 425, 1307
HgaI GACGC 2 cut(s) 331, 469
Hin1II CATG 7 cut(s) 95, 121, 203, 332, 487, 645, 1164
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 5 cut(s) 227, 449, 526, 698, 1202
HpaII CCGG 3 cut(s) 63, 425, 1307
HphI GGTGA 7 cut(s) 45, 244, 414, 485, 666, 772, 1193
Hpy166II GTNNAC 4 cut(s) 6, 1069, 1387, 1557
Hpy188I TCNGA 6 cut(s) 391, 703, 817, 1050, 1177, 1496
Hpy188III TCNNGA 8 cut(s) 63, 413, 530, 631, 793, 1142, 1372, 1486
Hpy8I GTNNAC 4 cut(s) 6, 1069, 1387, 1557
HpyAV CCTTC 6 cut(s) 216, 361, 575, 1069, 1432, 1492
HpyCH4III ACNGT 7 cut(s) 71, 581, 596, 693, 784, 965, 1396
HpyCH4IV ACGT 2 cut(s) 489, 617
HpyCH4V TGCA 5 cut(s) 645, 660, 1238, 1265, 1379
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 570, 1235
HpyF3I CTNAG 7 cut(s) 14, 388, 801, 814, 927, 1365, 1413
HpySE526I ACGT 2 cut(s) 489, 617
Hsp92II CATG 7 cut(s) 95, 121, 203, 332, 487, 645, 1164
Kpn2I TCCGGA 1 cut(s) 62
Kzo9I GATC 8 cut(s) 202, 289, 607, 679, 1110, 1245, 1315, 1488
LmnI GCTCC 3 cut(s) 407, 415, 561
Lsp1109I GCAGC 2 cut(s) 672, 1011
LweI GCATC 2 cut(s) 129, 647
MaeI CTAG 6 cut(s) 654, 1124, 1142, 1463, 1545, 1550
MaeII ACGT 2 cut(s) 489, 617
MaeIII GTNAC 1 cut(s) 542
MalI GATC 8 cut(s) 204, 291, 609, 681, 1112, 1247, 1317, 1490
MboI GATC 8 cut(s) 202, 289, 607, 679, 1110, 1245, 1315, 1488
MhlI GDGCHC 4 cut(s) 412, 503, 719, 1371
MlsI TGGCCA 2 cut(s) 852, 948
MluCI AATT 6 cut(s) 124, 315, 1081, 1402, 1458, 1513
MluNI TGGCCA 2 cut(s) 852, 948
MlyI GAGTC 1 cut(s) 458
Mox20I TGGCCA 2 cut(s) 852, 948
MroI TCCGGA 1 cut(s) 62
MroXI GAANNNNTTC 2 cut(s) 79, 1421
MscI TGGCCA 2 cut(s) 852, 948
MseI TTAA 7 cut(s) 114, 123, 252, 318, 548, 786, 1217
MslI CAYNNNNRTG 1 cut(s) 488
Msp20I TGGCCA 2 cut(s) 852, 948
MspCI CTTAAG 1 cut(s) 1216
MspI CCGG 3 cut(s) 63, 425, 1307
MspR9I CCNGG 2 cut(s) 426, 1307
MvnI CGCG 1 cut(s) 767
MwoI GCNNNNNNNGC 3 cut(s) 177, 570, 1235
NciI CCSGG 2 cut(s) 426, 1307
NdeI CATATG 1 cut(s) 1240
NdeII GATC 8 cut(s) 202, 289, 607, 679, 1110, 1245, 1315, 1488
NlaIII CATG 7 cut(s) 95, 121, 203, 332, 487, 645, 1164
NlaIV GGNNCC 3 cut(s) 557, 860, 1192
NmuCI GTSAC 1 cut(s) 542
NspI RCATGY 2 cut(s) 121, 645
PciI ACATGT 1 cut(s) 117
PdmI GAANNNNTTC 2 cut(s) 79, 1421
PfeI GAWTC 4 cut(s) 227, 526, 698, 1202
PkrI GCNGC 3 cut(s) 662, 1001, 1133
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 908
PscI ACATGT 1 cut(s) 117
Psp124BI GAGCTC 2 cut(s) 412, 1371
Psp5II RGGWCCY 1 cut(s) 908
PspN4I GGNNCC 3 cut(s) 557, 860, 1192
PspPI GGNCC 2 cut(s) 908, 1309
PspPPI RGGWCCY 1 cut(s) 908
PstNI CAGNNNCTG 1 cut(s) 1235
RsaI GTAC 3 cut(s) 690, 821, 1210
RsaNI GTAC 3 cut(s) 689, 820, 1209
RseI CAYNNNNRTG 1 cut(s) 488
SacI GAGCTC 2 cut(s) 412, 1371
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 7 cut(s) 114, 123, 252, 318, 548, 786, 1217
SatI GCNGC 3 cut(s) 661, 1000, 1132
Sau3AI GATC 8 cut(s) 202, 289, 607, 679, 1110, 1245, 1315, 1488
Sau96I GGNCC 2 cut(s) 908, 1309
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 426, 1307
SduI GDGCHC 4 cut(s) 412, 503, 719, 1371
SfaNI GCATC 2 cut(s) 129, 647
SfcI CTRYAG 1 cut(s) 70
SinI GGWCC 2 cut(s) 908, 1309
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 2 cut(s) 446, 1216
SmoI CTYRAG 2 cut(s) 446, 1216
Sse9I AATT 6 cut(s) 124, 315, 1081, 1402, 1458, 1513
SsiI CCGC 5 cut(s) 180, 621, 767, 1131, 1574
SspI AATATT 1 cut(s) 1293
SspMI CTAG 6 cut(s) 654, 1124, 1142, 1463, 1545, 1550
SstI GAGCTC 2 cut(s) 412, 1371
StyD4I CCNGG 2 cut(s) 424, 1305
StyI CCWWGG 2 cut(s) 192, 210
TaaI ACNGT 7 cut(s) 71, 581, 596, 693, 784, 965, 1396
TaiI ACGT 2 cut(s) 492, 620
TaqI TCGA 5 cut(s) 5, 347, 746, 972, 1487
TasI AATT 6 cut(s) 124, 315, 1081, 1402, 1458, 1513
TatI WGTACW 2 cut(s) 688, 819
TauI GCSGC 1 cut(s) 1134
TfiI GAWTC 4 cut(s) 227, 526, 698, 1202
Tru1I TTAA 7 cut(s) 114, 123, 252, 318, 548, 786, 1217
Tru9I TTAA 7 cut(s) 114, 123, 252, 318, 548, 786, 1217
TscAI CASTG 4 cut(s) 698, 805, 880, 1092
TseFI GTSAC 1 cut(s) 542
TseI GCWGC 2 cut(s) 660, 999
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 6 cut(s) 96, 317, 472, 615, 1289, 1414
TspRI CASTG 4 cut(s) 698, 805, 880, 1092
Vha464I CTTAAG 1 cut(s) 1216
VpaK11BI GGWCC 2 cut(s) 908, 1309
XapI RAATTY 3 cut(s) 1081, 1402, 1513
XbaI TCTAGA 1 cut(s) 1141
XceI RCATGY 2 cut(s) 121, 645
XmiI GTMKAC 2 cut(s) 5, 1386
XmnI GAANNNNTTC 2 cut(s) 79, 1421
XspI CTAG 6 cut(s) 654, 1124, 1142, 1463, 1545, 1550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.