MD01G1081400.v1.1

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013789
Physical Location & Seq
Forward (+)
18830157 .. 18830702
546 bp
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UTR
Exon/CDS
Intron
MD01G1081400.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGCGTCAACTCTGATATTCTCCCACTTACTAATTTCAATCATGATCACCATACCTCCATCGTCCATGTCTTCTCCTAAGACACCAGTCCGAGGTTTCGAGGCACGTCTCATTCACCGCGACTCTCCTCAATCTCCGTTCTACAACCTCAAGGCCACACCTACCAATCGCATCAAATCAGCACGCCGCAGAATCATAGCTAGACAAAACTACTTCAAATGGCTAATGTCAGGCAAAACACAGCGCAATTCCATCAGCACGCCGATTGATACAGACTATGGAGACAACATCATGAGGTTCAAAGTTGGCACCCCAAGAGTTGACACCTTTGGGATTTTCAACACGGCCAGCGATTTAATTTGGTTTCAATGTAAGCCTTGTGAAAAATGCTACGAACAAGGCATACCAATATTTGATCCTGCAAACTCAGACAGCTATCAGAAGGTAATGTGCGGCTCCATCGAGTGCAATACAACCTCAGATGCCCATTGCCCTGATCCAAAAGGCGAATGCCGGTACAAAATTGCGTACCAAGATGGATACTGA

Protein Analysis

182

Amino Acids

20.57

Weight (kDa)

9.1

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 97 - 180 5.5e-21 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42980
malus_domestica MD01G1081400.v1.1 MD01G1081500.v1.1 MD01G1081600.v1.1 MD01G1081900.v1.1
prunus_persica Prupe.2G189100_v2.0.a1
pyrus_communis pycom07g14880
rosa_chinensis RchiOBHm_Chr1g0359581
rosa_laevigata RLG00000027860
rosa_multiflora Rmu_sc0002556.1_g000022
rosa_roxburghii Rroxscaffold_4G00296910
rosa_samantha Rh1AG285600 Rh1BG251700 Rh1BG251800 Rh1DG280900 Rh1DG281000
rosa_wichuraiana Rw1G025390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 1 cut(s) 120
AciI CCGC 3 cut(s) 118, 187, 453
AclWI GGATC 2 cut(s) 410, 491
AcoI YGGCCR 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 518, 530
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 5 cut(s) 39, 217, 301, 340, 368
AjiI CACGTC 1 cut(s) 107
AluBI AGCT 2 cut(s) 200, 435
AluI AGCT 2 cut(s) 200, 435
Alw26I GTCTC 2 cut(s) 113, 276
AlwI GGATC 2 cut(s) 410, 491
AoxI GGCC 2 cut(s) 153, 345
AspLEI GCGC 1 cut(s) 246
AsuHPI GGTGA 2 cut(s) 40, 107
BanI GGYRCC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 63
BccI CCATC 4 cut(s) 67, 260, 467, 530
BceAI ACGGC 1 cut(s) 360
BciVI GTATCC 1 cut(s) 533
BclI TGATCA 1 cut(s) 45
BcoDI GTCTC 2 cut(s) 113, 276
BfaI CTAG 1 cut(s) 201
BfuI GTATCC 1 cut(s) 533
BisI GCNGC 2 cut(s) 187, 454
BlsI GCNGC 2 cut(s) 188, 455
BmgBI CACGTC 1 cut(s) 107
BmiI GGNNCC 2 cut(s) 310, 457
BmsI GCATC 2 cut(s) 180, 472
BoxI GACNNNNGTC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse118I RCCGGY 1 cut(s) 513
Bse1I ACTGG 1 cut(s) 86
Bse3DI GCAATG 1 cut(s) 487
BseDI CCNNGG 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 92
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 2 cut(s) 441, 492
BseNI ACTGG 1 cut(s) 86
BseRI GAGGAG 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 155, 347
BshNI GGYRCC 1 cut(s) 308
BsiSI CCGG 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 92
BsmAI GTCTC 2 cut(s) 113, 276
BsmBI CGTCTC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 2 cut(s) 155, 347
Bsp143I GATC 3 cut(s) 45, 415, 496
BspACI CCGC 3 cut(s) 118, 187, 453
BspANI GGCC 2 cut(s) 155, 347
BspCNI CTCAG 2 cut(s) 440, 491
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 2 cut(s) 42, 291
BspLI GGNNCC 2 cut(s) 310, 457
BspPI GGATC 2 cut(s) 410, 491
BspT107I GGYRCC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 513
BsrI ACTGG 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 513
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 3 cut(s) 45, 415, 496
BssNI GRCGYC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 3 cut(s) 184, 260, 349
BstDEI CTNAG 3 cut(s) 78, 427, 478
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 3 cut(s) 48, 418, 499
BstMAI GTCTC 2 cut(s) 113, 276
BstMBI GATC 3 cut(s) 45, 415, 496
BstPAI GACNNNNGTC 1 cut(s) 86
BstUI CGCG 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 63
BsuI GTATCC 1 cut(s) 533
BsuRI GGCC 2 cut(s) 155, 347
BtrI CACGTC 1 cut(s) 107
Cac8I GCNNGC 3 cut(s) 184, 260, 349
CciI TCATGA 2 cut(s) 42, 291
CfoI GCGC 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 513
Csp6I GTAC 2 cut(s) 517, 529
CviAII CATG 3 cut(s) 43, 67, 292
CviJI RGCY 7 cut(s) 155, 200, 223, 347, 376, 435, 456
CviKI_1 RGCY 7 cut(s) 155, 200, 223, 347, 376, 435, 456
CviQI GTAC 2 cut(s) 517, 529
DdeI CTNAG 3 cut(s) 78, 427, 478
DpnI GATC 3 cut(s) 47, 417, 498
DpnII GATC 3 cut(s) 45, 415, 496
EaeI YGGCCR 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 3 cut(s) 46, 70, 295
FaiI YATR 7 cut(s) 44, 53, 68, 197, 279, 293, 404
FatI CATG 3 cut(s) 42, 66, 291
FbaI TGATCA 1 cut(s) 45
Fnu4HI GCNGC 2 cut(s) 187, 454
Fsp4HI GCNGC 2 cut(s) 187, 454
FspBI CTAG 1 cut(s) 201
GlaI GCGC 1 cut(s) 245
GluI GCNGC 2 cut(s) 187, 454
HaeIII GGCC 2 cut(s) 155, 347
HapII CCGG 1 cut(s) 514
HhaI GCGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 46, 70, 295
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HincII GTYRAC 2 cut(s) 9, 322
HindII GTYRAC 2 cut(s) 9, 322
HinfI GANTC 2 cut(s) 122, 192
HpaII CCGG 1 cut(s) 514
HphI GGTGA 2 cut(s) 40, 107
Hpy166II GTNNAC 2 cut(s) 9, 322
Hpy188I TCNGA 5 cut(s) 15, 92, 430, 441, 481
Hpy188III TCNNGA 2 cut(s) 43, 292
Hpy8I GTNNAC 2 cut(s) 9, 322
HpyAV CCTTC 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 2 cut(s) 422, 468
HpyF3I CTNAG 3 cut(s) 78, 427, 478
HpySE526I ACGT 1 cut(s) 106
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 46, 70, 295
HspAI GCGC 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 45
Kzo9I GATC 3 cut(s) 45, 415, 496
LmnI GCTCC 1 cut(s) 461
LpnPI CCDG 6 cut(s) 99, 216, 361, 432, 507, 527
LweI GCATC 2 cut(s) 180, 472
MaeI CTAG 1 cut(s) 201
MaeII ACGT 1 cut(s) 106
MalI GATC 3 cut(s) 47, 417, 498
MboI GATC 3 cut(s) 45, 415, 496
MboII GAAGA 1 cut(s) 63
MluCI AATT 4 cut(s) 33, 247, 357, 522
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 7 cut(s) 66, 86, 94, 138, 158, 288, 487
MseI TTAA 1 cut(s) 356
MspI CCGG 1 cut(s) 514
Mva1269I GAATGC 1 cut(s) 515
MvnI CGCG 1 cut(s) 120
NdeII GATC 3 cut(s) 45, 415, 496
NlaIII CATG 3 cut(s) 46, 70, 295
NlaIV GGNNCC 2 cut(s) 310, 457
PagI TCATGA 2 cut(s) 42, 291
PctI GAATGC 1 cut(s) 515
PfeI GAWTC 1 cut(s) 192
PkrI GCNGC 2 cut(s) 188, 455
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PshAI GACNNNNGTC 1 cut(s) 86
PspN4I GGNNCC 2 cut(s) 310, 457
PsrI GAACNNNNNNTAC 2 cut(s) 387, 419
RsaI GTAC 2 cut(s) 518, 530
RsaNI GTAC 2 cut(s) 517, 529
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 2 cut(s) 187, 454
Sau3AI GATC 3 cut(s) 45, 415, 496
SchI GAGTC 1 cut(s) 116
SfaNI GCATC 2 cut(s) 180, 472
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 4 cut(s) 33, 247, 357, 522
SsiI CCGC 3 cut(s) 118, 187, 453
SspI AATATT 1 cut(s) 411
SspMI CTAG 1 cut(s) 201
TaiI ACGT 1 cut(s) 109
TaqI TCGA 2 cut(s) 99, 462
TasI AATT 4 cut(s) 33, 247, 357, 522
TauI GCSGC 2 cut(s) 189, 456
TfiI GAWTC 1 cut(s) 192
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TspGWI ACGGA 1 cut(s) 126
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.