pycom07g14880

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013789
Physical Location & Seq
Forward (+)
16915576 .. 16916627
1052 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g14880.3

Sequence Viewer

Length: 729 bp
ATGTCTTCTCCTGAGATACCAGTCCAAGGTTTCGAGGCACGTCTCATTCACCGCGACTCTCCTCAATCTCCGCTCTACAACCCCAAGGCCACACCTGCTAGCCGCATCAAATCAGCTCCCCGCATTTCCATCAGCGTGCCGATCGATAGAGACCATGAAGAAGTGATTGATAGAGACTATGGAGGCTACATCATGAGGCACAAAGTTGGCACAACAAGAGTCGACACCTTTGGGATTTTTCGACACGGCCAGCGATTTAATTTGGTTTCAGTCAGAGAGCCTTCTTCACTTGTCTGTCAGATTTCGTACCTCCACCTCTCGTATTGCATTTCATTCGAAAATAAAGGTACCTACAACAACAGCAACGTGGAGATGGAGGTGTTTTCGTGGACTCGGCAACAACTTATACCATATTTTGTTACGACGATAGCCGGTTTCAAGTGGATAAAACGCCGGATATTGTGTTTCATTTTACGGGACTTTCCGGTGACTGCGGATAACGCTTGGGCTATGAATTCTCAAGGACAATGCTGCCTGGTTCCAACAGCGGATGAAGTTTGGCCCTTCGTCGCAGATCTTTGCTACTTCCACAAATGGTTCCCAAATCTTGACACTTGTCAACTAGAGGGAGTACTCGGCAAACCTGGCCTGATACGGTACAGCGCAATCTACTCCGATGCCTTCGCTTCAACATCATCATCTGCCGATCAGGTGACGATCACAGATTGA

Protein Analysis

243

Amino Acids

27.53

Weight (kDa)

6.99

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42980
malus_domestica MD01G1081400.v1.1 MD01G1081500.v1.1 MD01G1081600.v1.1 MD01G1081900.v1.1
prunus_persica Prupe.2G189100_v2.0.a1
pyrus_communis pycom07g14880
rosa_chinensis RchiOBHm_Chr1g0359581
rosa_laevigata RLG00000027860
rosa_multiflora Rmu_sc0002556.1_g000022
rosa_roxburghii Rroxscaffold_4G00296910
rosa_samantha Rh1AG285600 Rh1BG251700 Rh1BG251800 Rh1DG280900 Rh1DG281000
rosa_wichuraiana Rw1G025390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 103
Acc65I GGTACC 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 347
AccBSI CCGCTC 1 cut(s) 73
AccI GTMKAC 1 cut(s) 222
AccII CGCG 1 cut(s) 54
AciI CCGC 6 cut(s) 52, 71, 103, 121, 494, 548
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 1 cut(s) 514
AfaI GTAC 4 cut(s) 308, 349, 633, 659
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 2 cut(s) 439, 690
AjiI CACGTC 1 cut(s) 41
AjnI CCWGG 2 cut(s) 534, 643
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
Alw26I GTCTC 3 cut(s) 47, 144, 168
AoxI GGCC 4 cut(s) 87, 247, 560, 646
ApeKI GCWGC 1 cut(s) 531
ApoI RAATTY 1 cut(s) 514
Asp718I GGTACC 1 cut(s) 347
AspLEI GCGC 1 cut(s) 665
AspS9I GGNCC 1 cut(s) 561
AsuHPI GGTGA 3 cut(s) 41, 499, 724
AsuII TTCGAA 1 cut(s) 336
AsuNHI GCTAGC 1 cut(s) 98
BanI GGYRCC 1 cut(s) 347
BbvI GCAGC 1 cut(s) 518
BccI CCATC 2 cut(s) 137, 367
BceAI ACGGC 1 cut(s) 262
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciT130I CCWGG 2 cut(s) 536, 645
BcoDI GTCTC 3 cut(s) 47, 144, 168
BfaI CTAG 2 cut(s) 99, 623
BfuAI ACCTGC 1 cut(s) 103
BglII AGATCT 1 cut(s) 574
BisI GCNGC 2 cut(s) 103, 532
BlsI GCNGC 2 cut(s) 104, 533
BmcAI AGTACT 1 cut(s) 633
Bme1390I CCNGG 2 cut(s) 536, 645
BmgBI CACGTC 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 561
BmiI GGNNCC 3 cut(s) 349, 540, 599
BmrFI CCNGG 2 cut(s) 536, 645
BmsI GCATC 2 cut(s) 114, 667
BmtI GCTAGC 1 cut(s) 102
BoxI GACNNNNGTC 1 cut(s) 615
Bpu14I TTCGAA 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 504
Bsa29I ATCGAT 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 25, 84
BsaWI WCCGGW 1 cut(s) 484
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 20
BseBI CCWGG 2 cut(s) 536, 645
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 25, 84
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 20
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 4 cut(s) 89, 249, 562, 648
BshNI GGYRCC 1 cut(s) 347
BshVI ATCGAT 1 cut(s) 144
BsiEI CGRYCG 1 cut(s) 144
BsiSI CCGG 3 cut(s) 432, 454, 485
BslFI GGGAC 1 cut(s) 491
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 3 cut(s) 47, 144, 168
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 491
BsnI GGCC 4 cut(s) 89, 249, 562, 648
Bso31I GGTCTC 1 cut(s) 144
Bsp119I TTCGAA 1 cut(s) 336
Bsp143I GATC 4 cut(s) 141, 574, 706, 717
BspACI CCGC 6 cut(s) 52, 71, 103, 121, 494, 548
BspANI GGCC 4 cut(s) 89, 249, 562, 648
BspCNI CTCAG 1 cut(s) 4
BspDI ATCGAT 1 cut(s) 144
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 3 cut(s) 349, 540, 599
BspMI ACCTGC 1 cut(s) 103
BspOI GCTAGC 1 cut(s) 102
BspT104I TTCGAA 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 144
BsrBI CCGCTC 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 25, 84
BssMI GATC 4 cut(s) 141, 574, 706, 717
BssT1I CCWWGG 2 cut(s) 25, 84
Bst2UI CCWGG 2 cut(s) 536, 645
Bst4CI ACNGT 1 cut(s) 657
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 100, 137, 251
BstDEI CTNAG 1 cut(s) 12
BstF5I GGATG 1 cut(s) 556
BstFNI CGCG 1 cut(s) 54
BstHHI GCGC 1 cut(s) 665
BstKTI GATC 4 cut(s) 144, 577, 709, 720
BstMAI GTCTC 3 cut(s) 47, 144, 168
BstMBI GATC 4 cut(s) 141, 574, 706, 717
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 3 cut(s) 95, 500, 645
BstNI CCWGG 2 cut(s) 536, 645
BstPAI GACNNNNGTC 1 cut(s) 615
BstSCI CCNGG 2 cut(s) 534, 643
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 518
BstX2I RGATCY 1 cut(s) 574
BstYI RGATCY 1 cut(s) 574
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 4 cut(s) 89, 249, 562, 648
BsuTUI ATCGAT 1 cut(s) 144
BtrI CACGTC 1 cut(s) 41
BtsCI GGATG 1 cut(s) 556
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 3 cut(s) 100, 137, 251
CciI TCATGA 1 cut(s) 192
CfoI GCGC 1 cut(s) 665
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 561
ClaI ATCGAT 1 cut(s) 144
Csp6I GTAC 4 cut(s) 307, 348, 632, 658
CviAII CATG 2 cut(s) 155, 193
CviQI GTAC 4 cut(s) 307, 348, 632, 658
DdeI CTNAG 1 cut(s) 12
DpnI GATC 4 cut(s) 143, 576, 708, 719
DpnII GATC 4 cut(s) 141, 574, 706, 717
EaeI YGGCCR 1 cut(s) 247
Eco130I CCWWGG 2 cut(s) 25, 84
Eco31I GGTCTC 1 cut(s) 144
EcoRI GAATTC 1 cut(s) 514
EcoRII CCWGG 2 cut(s) 534, 643
EcoT14I CCWWGG 2 cut(s) 25, 84
ErhI CCWWGG 2 cut(s) 25, 84
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 2 cut(s) 158, 196
FaiI YATR 6 cut(s) 156, 180, 194, 407, 412, 512
FaqI GGGAC 1 cut(s) 491
FatI CATG 2 cut(s) 154, 192
FauI CCCGC 1 cut(s) 128
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 2 cut(s) 103, 532
FokI GGATG 1 cut(s) 563
Fsp4HI GCNGC 2 cut(s) 103, 532
FspBI CTAG 2 cut(s) 99, 623
GlaI GCGC 1 cut(s) 664
GluI GCNGC 2 cut(s) 103, 532
HaeIII GGCC 4 cut(s) 89, 249, 562, 648
HapII CCGG 3 cut(s) 432, 454, 485
HhaI GCGC 1 cut(s) 665
Hin1II CATG 2 cut(s) 158, 196
Hin6I GCGC 1 cut(s) 663
HinP1I GCGC 1 cut(s) 663
HincII GTYRAC 2 cut(s) 223, 620
HindII GTYRAC 2 cut(s) 223, 620
HinfI GANTC 3 cut(s) 56, 219, 391
HpaII CCGG 3 cut(s) 432, 454, 485
HphI GGTGA 3 cut(s) 41, 499, 724
Hpy166II GTNNAC 3 cut(s) 223, 390, 620
Hpy188I TCNGA 3 cut(s) 275, 300, 676
Hpy188III TCNNGA 3 cut(s) 11, 193, 608
Hpy8I GTNNAC 3 cut(s) 223, 390, 620
Hpy99I CGWCG 2 cut(s) 427, 572
HpyAV CCTTC 3 cut(s) 291, 574, 691
HpyCH4III ACNGT 1 cut(s) 657
HpyCH4IV ACGT 2 cut(s) 40, 366
HpyCH4V TGCA 1 cut(s) 327
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 500, 645
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 2 cut(s) 40, 366
Hsp92II CATG 2 cut(s) 158, 196
HspAI GCGC 1 cut(s) 663
KpnI GGTACC 1 cut(s) 351
Kzo9I GATC 4 cut(s) 141, 574, 706, 717
LmnI GCTCC 1 cut(s) 121
Lsp1109I GCAGC 1 cut(s) 518
LweI GCATC 2 cut(s) 114, 667
MaeI CTAG 2 cut(s) 99, 623
MaeII ACGT 2 cut(s) 40, 366
MaeIII GTNAC 3 cut(s) 418, 487, 712
MalI GATC 4 cut(s) 143, 576, 708, 719
MbiI CCGCTC 1 cut(s) 73
MboI GATC 4 cut(s) 141, 574, 706, 717
MboII GAAGA 2 cut(s) 170, 276
MflI RGATCY 1 cut(s) 574
MluCI AATT 2 cut(s) 259, 514
MlyI GAGTC 3 cut(s) 50, 228, 385
MmeI TCCRAC 1 cut(s) 566
MnlI CCTC 8 cut(s) 28, 72, 176, 189, 320, 326, 370, 619
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 3 cut(s) 432, 454, 485
MspR9I CCNGG 2 cut(s) 536, 645
MvaI CCWGG 2 cut(s) 536, 645
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 3 cut(s) 95, 500, 645
NdeII GATC 4 cut(s) 141, 574, 706, 717
NheI GCTAGC 1 cut(s) 98
NlaIII CATG 2 cut(s) 158, 196
NlaIV GGNNCC 3 cut(s) 349, 540, 599
NmeAIII GCCGAG 2 cut(s) 373, 615
NmuCI GTSAC 2 cut(s) 487, 712
NspV TTCGAA 1 cut(s) 336
PagI TCATGA 1 cut(s) 192
PaqCI CACCTGC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 104, 533
Ple19I CGATCG 1 cut(s) 144
PleI GAGTC 3 cut(s) 50, 227, 385
PpsI GAGTC 3 cut(s) 50, 227, 385
PshAI GACNNNNGTC 1 cut(s) 615
Psp6I CCWGG 2 cut(s) 534, 643
PspGI CCWGG 2 cut(s) 534, 643
PspN4I GGNNCC 3 cut(s) 349, 540, 599
PspPI GGNCC 1 cut(s) 561
PsuI RGATCY 1 cut(s) 574
PvuI CGATCG 1 cut(s) 144
RsaI GTAC 4 cut(s) 308, 349, 633, 659
RsaNI GTAC 4 cut(s) 307, 348, 632, 658
RseI CAYNNNNRTG 1 cut(s) 134
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 2 cut(s) 103, 532
Sau3AI GATC 4 cut(s) 141, 574, 706, 717
Sau96I GGNCC 1 cut(s) 561
ScaI AGTACT 1 cut(s) 633
SchI GAGTC 3 cut(s) 50, 228, 385
ScrFI CCNGG 2 cut(s) 536, 645
SfaNI GCATC 2 cut(s) 114, 667
SfuI TTCGAA 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
Sse9I AATT 2 cut(s) 259, 514
SsiI CCGC 6 cut(s) 52, 71, 103, 121, 494, 548
SspMI CTAG 2 cut(s) 99, 623
StyD4I CCNGG 2 cut(s) 534, 643
StyI CCWWGG 2 cut(s) 25, 84
TaaI ACNGT 1 cut(s) 657
TaiI ACGT 2 cut(s) 43, 369
TaqI TCGA 5 cut(s) 33, 144, 222, 241, 336
TasI AATT 2 cut(s) 259, 514
TatI WGTACW 1 cut(s) 631
TauI GCSGC 1 cut(s) 105
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TseFI GTSAC 2 cut(s) 487, 712
TseI GCWGC 1 cut(s) 531
Tsp45I GTSAC 2 cut(s) 487, 712
TspDTI ATGAA 5 cut(s) 171, 321, 457, 527, 567
XapI RAATTY 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 2 cut(s) 99, 623
ZrmI AGTACT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.