Rw1G025390

Aspartic proteinase CDR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013789
Physical Location & Seq
Forward (+)
52013283 .. 52014612
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025390.1

Sequence Viewer

Length: 1191 bp
ATGCCCTCACCACTGATATTCTCCCTCATTGGACTCATTTTAGTCATCAACATCCCTCCCTCTATTTCTTCTCCTAAAACACCAGTCAAGGGTTTCGAGGCACGTCTCATTTACCGTGATTCTCCTGAATCTCCGCTCCACAACCACAAGGCAACGCATGCTGATCTTATCAAATCAGCTCGTCAGAGAACCTTGGCCAGACAAAATTACTTCAAAAGGCTAATGTCAGGTAAACCATGGCAGAAATTAATAAGTGCCCCCATTGATTTGGAACTAGGAGACTACATCATGAGGTTCCAAATTGGCACCCCAAAAGTGGATACCTATGCATTCTTCGACACTGCCAGCCGTTTGATTTGGTTGCACACACCAATATATGATCCTTCAAACTCACCAAGCTTTGTAGAAGTAAGGTGTAGCTCTCTAGACTGCAACATAACCGCAGATACGCAATGCCCCGATCCTAATGGTATATGCAAGTACACGATTGCGTATGGAGATGGAAGATCTTCTGAAGGAGTCCTTGCTAGAGAAACAATCACGCTCGAACATGATGGATCAAGTGGAAGCGCTAGCTTACCAAATATGATTTTCGGGTGCAGTCACTCTAATGTAGACCAGAGATCAGGTTACAATCCACCCAGAATTGTGGGACTAAGCAGAGAACCTTCTTCCCTGGTAGGCCAAATTTCGTACCCTCATGTTTCGTATTGCATATCAGCTAATAATGAAGGCAACCATAGCTCTGTTAAGTTCGGCTCTGTAGCAGTCATCACTGAGAATACCACTCAAACCCCATTGTTAACTGGGGTCTTCATGGATACAGGAACAGATCATACTCAGCTGGTACTGCCAGCATTTTATGCAGCCACCAATAAAATAGAGGATATCTTGTCGGATTTCAAATCCTTACCAGATGTGGATGGCAGCTTTCCAGTGTGTTACAACGATACGAATTTTAGATTGGATTCGACACCAGAAATAATATTCCAATTTACAGGACTGGAGTTTCCGTTGAGTGCCAATAACACTTGGGATCAGAACTCTCTGGGATACTATTGTTTGGCTATATTGCCAGCATTTAGGGGTCATTCTGTTCTTGGAATGTACCAGCAAAGGAACGTAAATGTTGGATATGACTTCAATAACAACATTGTATCTTTGAAGTATAGCGACAATAACTGCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

396

Amino Acids

43.75

Weight (kDa)

5.79

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 95 - 254 1.4e-32 Xylanase inhibitor N-terminal
TAXi_C PF14541 269 - 386 3.5e-12 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42980
malus_domestica MD01G1081400.v1.1 MD01G1081500.v1.1 MD01G1081600.v1.1 MD01G1081900.v1.1
prunus_persica Prupe.2G189100_v2.0.a1
pyrus_communis pycom07g14880
rosa_chinensis RchiOBHm_Chr1g0359581
rosa_laevigata RLG00000027860
rosa_multiflora Rmu_sc0002556.1_g000022
rosa_roxburghii Rroxscaffold_4G00296910
rosa_samantha Rh1AG285600 Rh1BG251700 Rh1BG251800 Rh1DG280900 Rh1DG281000
rosa_wichuraiana Rw1G025390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 615
AciI CCGC 2 cut(s) 134, 441
AclWI GGATC 4 cut(s) 374, 455, 565, 1044
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 2 cut(s) 687, 955
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 4 cut(s) 482, 695, 849, 1109
AfeI AGCGCT 1 cut(s) 571
AfiI CCNNNNNNNGG 2 cut(s) 89, 316
AgsI TTSAA 5 cut(s) 214, 387, 904, 1144, 1165
AjiI CACGTC 1 cut(s) 104
AjnI CCWGG 1 cut(s) 675
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AluBI AGCT 8 cut(s) 179, 399, 420, 576, 722, 744, 844, 930
AluI AGCT 8 cut(s) 179, 399, 420, 576, 722, 744, 844, 930
Alw26I GTCTC 2 cut(s) 110, 273
AlwI GGATC 4 cut(s) 374, 455, 565, 1044
Aor51HI AGCGCT 1 cut(s) 571
AoxI GGCC 2 cut(s) 195, 682
ApeKI GCWGC 2 cut(s) 866, 927
ApoI RAATTY 2 cut(s) 687, 955
AseI ATTAAT 1 cut(s) 248
Asp700I GAANNNNTTC 1 cut(s) 508
AspLEI GCGC 2 cut(s) 572, 1187
AsuHPI GGTGA 1 cut(s) 384
AsuNHI GCTAGC 1 cut(s) 572
BaeGI GKGCMC 1 cut(s) 259
BaeI ACNNNNGTAYC 2 cut(s) 1045, 1078
BalI TGGCCA 1 cut(s) 197
BanI GGYRCC 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 805
BbvI GCAGC 2 cut(s) 878, 939
BccI CCATC 3 cut(s) 494, 548, 917
BceAI ACGGC 1 cut(s) 333
BciT130I CCWGG 1 cut(s) 677
BciVI GTATCC 3 cut(s) 313, 814, 1046
BcoDI GTCTC 2 cut(s) 110, 273
BfaI CTAG 4 cut(s) 275, 425, 528, 573
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 1 cut(s) 573
BfuI GTATCC 3 cut(s) 313, 814, 1046
BglII AGATCT 1 cut(s) 506
BisI GCNGC 2 cut(s) 867, 928
BlsI GCNGC 2 cut(s) 868, 929
Bme1390I CCNGG 1 cut(s) 677
BmgBI CACGTC 1 cut(s) 104
BmiI GGNNCC 2 cut(s) 296, 307
BmrFI CCNGG 1 cut(s) 677
BmrI ACTGGG 1 cut(s) 816
BmtI GCTAGC 1 cut(s) 576
BmuI ACTGGG 1 cut(s) 816
BpiI GAAGAC 1 cut(s) 805
BpmI CTGGAG 1 cut(s) 1025
BsaJI CCNNGG 3 cut(s) 192, 236, 675
BsaXI ACNNNNNCTCC 2 cut(s) 998, 1028
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 316
Bse1I ACTGG 4 cut(s) 83, 811, 935, 1008
Bse3DI GCAATG 1 cut(s) 458
BseBI CCWGG 1 cut(s) 677
BseDI CCNNGG 3 cut(s) 192, 236, 675
BseGI GGATG 2 cut(s) 51, 928
BseLI CCNNNNNNNGG 2 cut(s) 89, 316
BseMI GCAATG 1 cut(s) 458
BseMII CTCAG 2 cut(s) 768, 854
BseNI ACTGG 4 cut(s) 83, 811, 935, 1008
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 2 cut(s) 878, 939
BsgI GTGCAG 1 cut(s) 619
BshFI GGCC 2 cut(s) 197, 684
BshNI GGYRCC 1 cut(s) 305
BslFI GGGAC 1 cut(s) 666
BslI CCNNNNNNNGG 2 cut(s) 89, 316
BsmAI GTCTC 2 cut(s) 110, 273
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 666
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 2 cut(s) 197, 684
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 8 cut(s) 163, 379, 460, 506, 557, 623, 832, 1036
Bsp19I CCATGG 1 cut(s) 236
BspACI CCGC 2 cut(s) 134, 441
BspANI GGCC 2 cut(s) 197, 684
BspCNI CTCAG 2 cut(s) 769, 853
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 296, 307
BspOI GCTAGC 1 cut(s) 576
BspPI GGATC 4 cut(s) 374, 455, 565, 1044
BspT107I GGYRCC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 458
BsrI ACTGG 4 cut(s) 83, 811, 935, 1008
BssECI CCNNGG 3 cut(s) 192, 236, 675
BssMI GATC 8 cut(s) 163, 379, 460, 506, 557, 623, 832, 1036
BssT1I CCWWGG 2 cut(s) 192, 236
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 1 cut(s) 116
BstAPI GCANNNNNTGC 2 cut(s) 158, 863
BstC8I GCNNGC 5 cut(s) 159, 346, 574, 855, 1077
BstDEI CTNAG 3 cut(s) 656, 777, 840
BstDSI CCRYGG 1 cut(s) 236
BstF5I GGATG 2 cut(s) 51, 928
BstH2I RGCGCY 1 cut(s) 573
BstHHI GCGC 2 cut(s) 572, 1187
BstKTI GATC 8 cut(s) 166, 382, 463, 509, 560, 626, 835, 1039
BstMAI GTCTC 2 cut(s) 110, 273
BstMBI GATC 8 cut(s) 163, 379, 460, 506, 557, 623, 832, 1036
BstMWI GCNNNNNNNGC 4 cut(s) 158, 741, 850, 863
BstNI CCWGG 1 cut(s) 677
BstNSI RCATGY 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 259
BstV1I GCAGC 2 cut(s) 878, 939
BstV2I GAAGAC 1 cut(s) 805
BstX2I RGATCY 1 cut(s) 506
BstXI CCANNNNNNTGG 2 cut(s) 268, 649
BstYI RGATCY 1 cut(s) 506
BsuI GTATCC 3 cut(s) 313, 814, 1046
BsuRI GGCC 2 cut(s) 197, 684
BtgI CCRYGG 1 cut(s) 236
BtrI CACGTC 1 cut(s) 104
BtsCI GGATG 2 cut(s) 51, 928
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 4 cut(s) 11, 339, 774, 942
Cac8I GCNNGC 5 cut(s) 159, 346, 574, 855, 1077
CciI TCATGA 1 cut(s) 288
CfoI GCGC 2 cut(s) 572, 1187
Csp6I GTAC 4 cut(s) 481, 694, 848, 1108
CviAII CATG 6 cut(s) 158, 237, 289, 551, 701, 817
CviQI GTAC 4 cut(s) 481, 694, 848, 1108
DdeI CTNAG 3 cut(s) 656, 777, 840
DpnI GATC 8 cut(s) 165, 381, 462, 508, 559, 625, 834, 1038
DpnII GATC 8 cut(s) 163, 379, 460, 506, 557, 623, 832, 1036
EaeI YGGCCR 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 192, 236
Eco32I GATATC 1 cut(s) 889
Eco47III AGCGCT 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 534
EcoRII CCWGG 1 cut(s) 675
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 2 cut(s) 192, 236
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 2 cut(s) 192, 236
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 6 cut(s) 161, 240, 292, 554, 704, 820
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 1 cut(s) 666
FatI CATG 6 cut(s) 157, 236, 288, 550, 700, 816
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 2 cut(s) 867, 928
FokI GGATG 2 cut(s) 38, 935
Fsp4HI GCNGC 2 cut(s) 867, 928
FspBI CTAG 4 cut(s) 275, 425, 528, 573
GlaI GCGC 2 cut(s) 571, 1186
GluI GCNGC 2 cut(s) 867, 928
GsuI CTGGAG 1 cut(s) 1025
HaeII RGCGCY 1 cut(s) 573
HaeIII GGCC 2 cut(s) 197, 684
HhaI GCGC 2 cut(s) 572, 1187
Hin1II CATG 6 cut(s) 161, 240, 292, 554, 704, 820
Hin6I GCGC 2 cut(s) 570, 1185
HinP1I GCGC 2 cut(s) 570, 1185
HincII GTYRAC 1 cut(s) 804
HindII GTYRAC 1 cut(s) 804
HindIII AAGCTT 1 cut(s) 397
HinfI GANTC 5 cut(s) 33, 119, 128, 519, 968
HpaI GTTAAC 1 cut(s) 804
HphI GGTGA 1 cut(s) 384
Hpy166II GTNNAC 4 cut(s) 233, 483, 616, 804
Hpy188I TCNGA 4 cut(s) 186, 514, 898, 1041
Hpy188III TCNNGA 3 cut(s) 125, 289, 425
Hpy8I GTNNAC 4 cut(s) 233, 483, 616, 804
HpyAV CCTTC 4 cut(s) 393, 509, 678, 725
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 2 cut(s) 103, 1122
HpyCH4V TGCA 7 cut(s) 329, 364, 432, 477, 600, 714, 866
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 741, 850, 863
HpyF3I CTNAG 3 cut(s) 656, 777, 840
HpySE526I ACGT 2 cut(s) 103, 1122
Hsp92II CATG 6 cut(s) 161, 240, 292, 554, 704, 820
HspAI GCGC 2 cut(s) 570, 1185
KspAI GTTAAC 1 cut(s) 804
Kzo9I GATC 8 cut(s) 163, 379, 460, 506, 557, 623, 832, 1036
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 2 cut(s) 878, 939
MaeI CTAG 4 cut(s) 275, 425, 528, 573
MaeII ACGT 2 cut(s) 103, 1122
MaeIII GTNAC 3 cut(s) 602, 629, 941
MalI GATC 8 cut(s) 165, 381, 462, 508, 559, 625, 834, 1038
MbiI CCGCTC 1 cut(s) 136
MboI GATC 8 cut(s) 163, 379, 460, 506, 557, 623, 832, 1036
MboII GAAGA 6 cut(s) 60, 325, 501, 516, 663, 805
MflI RGATCY 1 cut(s) 506
MhlI GDGCHC 1 cut(s) 259
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 7 cut(s) 205, 245, 300, 645, 687, 955, 992
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 2 cut(s) 27, 528
MmeI TCCRAC 2 cut(s) 876, 1111
MnlI CCTC 8 cut(s) 16, 35, 66, 70, 91, 285, 708, 877
Mox20I TGGCCA 1 cut(s) 197
Mph1103I ATGCAT 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 508
MscI TGGCCA 1 cut(s) 197
MseI TTAA 3 cut(s) 248, 750, 803
MslI CAYNNNNRTG 1 cut(s) 609
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 844
MspR9I CCNGG 1 cut(s) 677
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 1 cut(s) 677
MwoI GCNNNNNNNGC 4 cut(s) 158, 741, 850, 863
NcoI CCATGG 1 cut(s) 236
NdeII GATC 8 cut(s) 163, 379, 460, 506, 557, 623, 832, 1036
NheI GCTAGC 1 cut(s) 572
NlaIII CATG 6 cut(s) 161, 240, 292, 554, 704, 820
NlaIV GGNNCC 2 cut(s) 296, 307
NmuCI GTSAC 1 cut(s) 602
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 1 cut(s) 161
PaeI GCATGC 1 cut(s) 161
PagI TCATGA 1 cut(s) 288
PctI GAATGC 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 119, 128, 968
PkrI GCNGC 2 cut(s) 868, 929
PleI GAGTC 2 cut(s) 27, 527
PpsI GAGTC 2 cut(s) 27, 527
PshBI ATTAAT 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 675
PspGI CCWGG 1 cut(s) 675
PspN4I GGNNCC 2 cut(s) 296, 307
PsrI GAACNNNNNNTAC 2 cut(s) 820, 852
PsuI RGATCY 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 844
RsaI GTAC 4 cut(s) 482, 695, 849, 1109
RsaNI GTAC 4 cut(s) 481, 694, 848, 1108
RseI CAYNNNNRTG 1 cut(s) 609
SaqAI TTAA 3 cut(s) 248, 750, 803
SatI GCNGC 2 cut(s) 867, 928
Sau3AI GATC 8 cut(s) 163, 379, 460, 506, 557, 623, 832, 1036
SchI GAGTC 2 cut(s) 27, 528
ScrFI CCNGG 1 cut(s) 677
SduI GDGCHC 1 cut(s) 259
SfcI CTRYAG 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 609
SphI GCATGC 1 cut(s) 161
Sse9I AATT 7 cut(s) 205, 245, 300, 645, 687, 955, 992
SsiI CCGC 2 cut(s) 134, 441
SspI AATATT 1 cut(s) 987
SspMI CTAG 4 cut(s) 275, 425, 528, 573
StyD4I CCNGG 1 cut(s) 675
StyI CCWWGG 2 cut(s) 192, 236
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 2 cut(s) 106, 1125
TaqI TCGA 4 cut(s) 96, 336, 546, 971
TasI AATT 7 cut(s) 205, 245, 300, 645, 687, 955, 992
TatI WGTACW 1 cut(s) 480
TfiI GAWTC 3 cut(s) 119, 128, 968
Tru1I TTAA 3 cut(s) 248, 750, 803
Tru9I TTAA 3 cut(s) 248, 750, 803
TscAI CASTG 4 cut(s) 18, 346, 781, 942
TseFI GTSAC 1 cut(s) 602
TseI GCWGC 2 cut(s) 866, 927
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 2 cut(s) 744, 805
TspGWI ACGGA 1 cut(s) 1002
TspRI CASTG 4 cut(s) 18, 346, 781, 942
VspI ATTAAT 1 cut(s) 248
XapI RAATTY 2 cut(s) 687, 955
XbaI TCTAGA 1 cut(s) 424
XceI RCATGY 1 cut(s) 161
XmiI GTMKAC 1 cut(s) 615
XmnI GAANNNNTTC 1 cut(s) 508
XspI CTAG 4 cut(s) 275, 425, 528, 573
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.