MD01G1081900.v1.1

Aspartic proteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013789
Physical Location & Seq
Forward (+)
18849265 .. 18850593
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1081900.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGGCCTCATCATTTGTACTCTCCTTCATTGCACTAATTTCAATAGCCATCACCATCCCCCTCTGCATGTCCTCTCCTAAAACAGCACTCCGAGGCTTCGAGACACGTCTCATTCATCGCGACTCGCCTGAATCTCCGCTATACAACCCTAGTCTCACTCGTGCTCAACGCAAAGAAGCAGCTCGTCGCAGAAGCATAGCCAGACAAAATTACCTCACAAGGCGAATGTCACCAGCAGCAGTAAGTAACGCTACTGCCCGCATTGATTACGATAATGGAGTCTACGTCATGAGGTACAAAATTGGAACCCCTCCAGTTGATACATTTGGATACTTTGACACTGCTAGCGCCTTAATTTGGATGCAGTGTAATCCCTGCAAAAAATGTTACAATCAAAGCATACCAATCTTTGAACCTGGAAAATCATCCTCCTACCGAAAGCTTATGTGTGACGATGACCGTGTGGAGTGTGGCCTAGTCCCAGATAACGGTTGCCCAAGTGAGGATGGTTCATGTACTTACATTACGGCTTATGAAGATGGAGCGTCTTCCGAAGGAGTCGCTTCCAAAGAAACATTCATGCTCGAAGGTGAAACAATCCCCGATATGACTTTCGGGTGTGGTCTGAAGAATTCAGATACGGATCCGGATGAAAATCCGCCAGGAGTTGTTGGTGCAGGTAGAGAACCAAGTTCACTTGTTGCGCAATTAATTAGAGAACGGACTCGTTTTTCGTTTTGCATTTCGCCTTTTAATCGAGGTAACCGTAGCAATGTGAAATTCGGTTCAAAGGCAGTGATCCATGAGGGTAATCAGACCCCATTATTACCTGGTCGATACGGTTGGTACCATATAGGGGTAGAAGGTATTAGCGTTGACGGTACTAGGCTCAACATTTCGGAGGATGTGTTCAGATGGAGGCCAGAGGGAAGAGGAGGCATCATCATAGATACAGGAACAACATATACTCACCTTGCATCGGAGGCTTATTACGCAGTCCAGAGCGCGGTGATAAATGCGCTACCGAATTACAAACACAAGATAAATCCCCATTCGGAGTTCCTGCTGTGTTATGATTCGGATGATGGCTTCCATATGAACATAGCACCTGATATAGAGTTCCATTTCAGAGATGATCGCCGACATGGGTTTGATTATACATTGGATCCGACTAATGTTTGGAATGAAGATGACTGCATGACTATAAAACCAGATTATCATGGCCTTTCTGTGTTGGGAATAAACCAGCTTGAGAATGTGAACGTCGGGATCGACCTTCAGAATAACATCGTAACTTTTAAGGACACTGATGACTGTCGGGATACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

443

Amino Acids

49.34

Weight (kDa)

5.37

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 95 - 263 3.5e-38 Xylanase inhibitor N-terminal
Asp PF00026 170 - 433 6.1e-06 Eukaryotic aspartyl protease
TAXi_C PF14541 283 - 434 4e-24 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42980
malus_domestica MD01G1081400.v1.1 MD01G1081500.v1.1 MD01G1081600.v1.1 MD01G1081900.v1.1
prunus_persica Prupe.2G189100_v2.0.a1
pyrus_communis pycom07g14880
rosa_chinensis RchiOBHm_Chr1g0359581
rosa_laevigata RLG00000027860
rosa_multiflora Rmu_sc0002556.1_g000022
rosa_roxburghii Rroxscaffold_4G00296910
rosa_samantha Rh1AG285600 Rh1BG251700 Rh1BG251800 Rh1DG280900 Rh1DG281000
rosa_wichuraiana Rw1G025390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 705
Acc36I ACCTGC 1 cut(s) 668
Acc65I GGTACC 1 cut(s) 846
AccB1I GGYRCC 1 cut(s) 846
AccI GTMKAC 1 cut(s) 282
AccII CGCG 2 cut(s) 120, 1007
AccIII TCCGGA 1 cut(s) 646
AciI CCGC 4 cut(s) 137, 259, 659, 1007
AclWI GGATC 6 cut(s) 638, 651, 793, 1160, 1173, 1277
AcsI RAATTY 2 cut(s) 631, 779
AcuI CTGAAG 2 cut(s) 647, 1262
AfaI GTAC 5 cut(s) 18, 296, 517, 848, 883
AfiI CCNNNNNNNGG 6 cut(s) 357, 488, 502, 856, 979, 1006
AflIII ACRYGT 1 cut(s) 104
AgsI TTSAA 3 cut(s) 42, 413, 789
AjiI CACGTC 1 cut(s) 107
AjnI CCWGG 3 cut(s) 415, 661, 829
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AloI GAACNNNNNNTCC 2 cut(s) 893, 925
AluBI AGCT 3 cut(s) 182, 442, 1249
AluI AGCT 3 cut(s) 182, 442, 1249
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 3 cut(s) 95, 113, 158
AlwI GGATC 6 cut(s) 638, 651, 793, 1160, 1173, 1277
Aor13HI TCCGGA 1 cut(s) 646
AoxI GGCC 4 cut(s) 3, 472, 920, 1222
ApeKI GCWGC 2 cut(s) 179, 236
ApoI RAATTY 2 cut(s) 631, 779
ArsI GACNNNNNNTTYG 2 cut(s) 715, 747
AseI ATTAAT 1 cut(s) 710
Asp718I GGTACC 1 cut(s) 846
AspLEI GCGC 4 cut(s) 350, 706, 1007, 1021
AsuHPI GGTGA 5 cut(s) 43, 222, 602, 962, 1021
AsuNHI GCTAGC 1 cut(s) 344
BamHI GGATCC 2 cut(s) 643, 1165
BanI GGYRCC 1 cut(s) 846
BauI CACGAG 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 191, 248
BccI CCATC 6 cut(s) 56, 62, 500, 533, 909, 1079
BceAI ACGGC 1 cut(s) 543
BciT130I CCWGG 3 cut(s) 417, 663, 831
BciVI GTATCC 2 cut(s) 323, 1315
BcoDI GTCTC 3 cut(s) 95, 113, 158
BfaI CTAG 4 cut(s) 150, 345, 476, 885
BfoI RGCGCY 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 668
BfuI GTATCC 2 cut(s) 323, 1315
BisI GCNGC 2 cut(s) 180, 237
BlsI GCNGC 2 cut(s) 181, 238
Bme1390I CCNGG 3 cut(s) 417, 663, 831
BmgBI CACGTC 1 cut(s) 107
BmiI GGNNCC 4 cut(s) 307, 645, 848, 1167
BmrFI CCNGG 3 cut(s) 417, 663, 831
BmsI GCATC 3 cut(s) 351, 948, 986
BmtI GCTAGC 1 cut(s) 348
BpiI GAAGAC 1 cut(s) 540
BpmI CTGGAG 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 1271
BsaBI GATNNNNATC 2 cut(s) 642, 654
BsaJI CCNNGG 1 cut(s) 91
BsaWI WCCGGW 1 cut(s) 646
BsaXI ACNNNNNCTCC 4 cut(s) 270, 300, 893, 923
Bsc4I CCNNNNNNNGG 6 cut(s) 357, 488, 502, 856, 979, 1006
Bse1I ACTGG 1 cut(s) 314
Bse3DI GCAATG 2 cut(s) 27, 778
Bse8I GATNNNNATC 2 cut(s) 642, 654
BseAI TCCGGA 1 cut(s) 646
BseBI CCWGG 3 cut(s) 417, 663, 831
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 7 cut(s) 54, 366, 425, 511, 655, 910, 1087
BseJI GATNNNNATC 2 cut(s) 642, 654
BseLI CCNNNNNNNGG 6 cut(s) 357, 488, 502, 856, 979, 1006
BseMI GCAATG 2 cut(s) 27, 778
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 948
BseXI GCAGC 2 cut(s) 191, 248
BsgI GTGCAG 1 cut(s) 696
Bsh1236I CGCG 2 cut(s) 120, 1007
BshFI GGCC 4 cut(s) 5, 474, 922, 1224
BshNI GGYRCC 1 cut(s) 846
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 1 cut(s) 647
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 6 cut(s) 357, 488, 502, 856, 979, 1006
BsmAI GTCTC 3 cut(s) 95, 113, 158
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 464
BsnI GGCC 4 cut(s) 5, 474, 922, 1224
Bsp1286I GDGCHC 1 cut(s) 166
Bsp13I TCCGGA 1 cut(s) 646
Bsp143I GATC 5 cut(s) 643, 798, 1135, 1165, 1269
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 4 cut(s) 137, 259, 659, 1007
BspANI GGCC 4 cut(s) 5, 474, 922, 1224
BspEI TCCGGA 1 cut(s) 646
BspFNI CGCG 2 cut(s) 120, 1007
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 4 cut(s) 307, 645, 848, 1167
BspMI ACCTGC 1 cut(s) 668
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 6 cut(s) 638, 651, 793, 1160, 1173, 1277
BspT107I GGYRCC 1 cut(s) 846
BsrDI GCAATG 2 cut(s) 27, 778
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 5 cut(s) 643, 798, 1135, 1165, 1269
BssSI CACGAG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 3 cut(s) 417, 663, 831
Bst4CI ACNGT 6 cut(s) 461, 491, 767, 842, 881, 1316
Bst6I CTCTTC 1 cut(s) 925
BstC8I GCNNGC 2 cut(s) 259, 346
BstEII GGTNACC 1 cut(s) 761
BstF5I GGATG 7 cut(s) 54, 366, 425, 511, 655, 910, 1087
BstFNI CGCG 2 cut(s) 120, 1007
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 4 cut(s) 350, 706, 1007, 1021
BstKTI GATC 5 cut(s) 646, 801, 1138, 1168, 1272
BstMAI GTCTC 3 cut(s) 95, 113, 158
BstMBI GATC 5 cut(s) 643, 798, 1135, 1165, 1269
BstMWI GCNNNNNNNGC 2 cut(s) 983, 992
BstNI CCWGG 3 cut(s) 417, 663, 831
BstNSI RCATGY 1 cut(s) 70
BstPI GGTNACC 1 cut(s) 761
BstSCI CCNGG 3 cut(s) 415, 661, 829
BstUI CGCG 2 cut(s) 120, 1007
BstV1I GCAGC 2 cut(s) 191, 248
BstV2I GAAGAC 1 cut(s) 540
BstX2I RGATCY 2 cut(s) 643, 1165
BstYI RGATCY 2 cut(s) 643, 1165
BsuI GTATCC 2 cut(s) 323, 1315
BsuRI GGCC 4 cut(s) 5, 474, 922, 1224
BtgZI GCGATG 1 cut(s) 101
BtrI CACGTC 1 cut(s) 107
BtsCI GGATG 7 cut(s) 54, 366, 425, 511, 655, 910, 1087
BtsI GCAGTG 3 cut(s) 339, 371, 801
BtsIMutI CAGTG 4 cut(s) 339, 371, 801, 1305
BtuMI TCGCGA 1 cut(s) 120
BveI ACCTGC 1 cut(s) 668
Cac8I GCNNGC 2 cut(s) 259, 346
CciI TCATGA 1 cut(s) 288
CfoI GCGC 4 cut(s) 350, 706, 1007, 1021
CseI GACGC 1 cut(s) 534
CsiI ACCWGGT 1 cut(s) 829
Csp6I GTAC 5 cut(s) 17, 295, 516, 847, 882
CviAII CATG 8 cut(s) 67, 289, 513, 580, 803, 1145, 1198, 1220
CviQI GTAC 5 cut(s) 17, 295, 516, 847, 882
DpnI GATC 5 cut(s) 645, 800, 1137, 1167, 1271
DpnII GATC 5 cut(s) 643, 798, 1135, 1165, 1269
Eam1104I CTCTTC 1 cut(s) 925
EarI CTCTTC 1 cut(s) 925
EciI GGCGGA 1 cut(s) 648
Eco57I CTGAAG 2 cut(s) 647, 1262
Eco91I GGTNACC 1 cut(s) 761
EcoO65I GGTNACC 1 cut(s) 761
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 3 cut(s) 415, 661, 829
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 8 cut(s) 70, 292, 516, 583, 806, 1148, 1201, 1223
FaqI GGGAC 1 cut(s) 464
FatI CATG 8 cut(s) 66, 288, 512, 579, 802, 1144, 1197, 1219
FauI CCCGC 1 cut(s) 266
FauNDI CATATG 1 cut(s) 1095
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 180, 237
FokI GGATG 7 cut(s) 41, 373, 412, 518, 662, 917, 1094
Fsp4HI GCNGC 2 cut(s) 180, 237
FspBI CTAG 4 cut(s) 150, 345, 476, 885
FspI TGCGCA 1 cut(s) 705
GlaI GCGC 4 cut(s) 349, 705, 1006, 1020
GluI GCNGC 2 cut(s) 180, 237
GsuI CTGGAG 1 cut(s) 297
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 4 cut(s) 5, 474, 922, 1224
HapII CCGG 1 cut(s) 647
HgaI GACGC 1 cut(s) 534
HhaI GCGC 4 cut(s) 350, 706, 1007, 1021
Hin1II CATG 8 cut(s) 70, 292, 516, 583, 806, 1148, 1201, 1223
Hin6I GCGC 4 cut(s) 348, 704, 1005, 1019
HinP1I GCGC 4 cut(s) 348, 704, 1005, 1019
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 6 cut(s) 122, 131, 279, 558, 724, 1076
HpaII CCGG 1 cut(s) 647
HphI GGTGA 5 cut(s) 43, 222, 602, 962, 1021
Hpy166II GTNNAC 4 cut(s) 283, 695, 877, 1261
Hpy188III TCNNGA 7 cut(s) 100, 119, 289, 647, 1000, 1267, 1319
Hpy8I GTNNAC 4 cut(s) 283, 695, 877, 1261
Hpy99I CGWCG 2 cut(s) 189, 1268
HpyAV CCTTC 5 cut(s) 34, 548, 581, 857, 1286
HpyCH4III ACNGT 6 cut(s) 461, 491, 767, 842, 881, 1316
HpyCH4IV ACGT 3 cut(s) 106, 285, 1263
HpyCH4V TGCA 8 cut(s) 32, 66, 364, 378, 677, 741, 977, 1197
HpyF10VI GCNNNNNNNGC 2 cut(s) 983, 992
HpySE526I ACGT 3 cut(s) 106, 285, 1263
Hsp92II CATG 8 cut(s) 70, 292, 516, 583, 806, 1148, 1201, 1223
HspAI GCGC 4 cut(s) 348, 704, 1005, 1019
Kpn2I TCCGGA 1 cut(s) 646
KpnI GGTACC 1 cut(s) 850
Kzo9I GATC 5 cut(s) 643, 798, 1135, 1165, 1269
LmnI GCTCC 1 cut(s) 542
Lsp1109I GCAGC 2 cut(s) 191, 248
LweI GCATC 3 cut(s) 351, 948, 986
MabI ACCWGGT 1 cut(s) 829
MaeI CTAG 4 cut(s) 150, 345, 476, 885
MaeII ACGT 3 cut(s) 106, 285, 1263
MaeIII GTNAC 6 cut(s) 228, 245, 386, 449, 761, 1291
MalI GATC 5 cut(s) 645, 800, 1137, 1167, 1271
MboI GATC 5 cut(s) 643, 798, 1135, 1165, 1269
MboII GAAGA 5 cut(s) 540, 548, 640, 942, 1199
MflI RGATCY 2 cut(s) 643, 1165
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 9 cut(s) 36, 208, 300, 354, 631, 707, 711, 779, 1027
MlyI GAGTC 4 cut(s) 116, 288, 567, 718
MmeI TCCRAC 1 cut(s) 1193
MroI TCCGGA 1 cut(s) 646
MseI TTAA 4 cut(s) 353, 710, 753, 1299
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 3 cut(s) 417, 663, 831
MvaI CCWGG 3 cut(s) 417, 663, 831
MvnI CGCG 2 cut(s) 120, 1007
MwoI GCNNNNNNNGC 2 cut(s) 983, 992
NdeI CATATG 1 cut(s) 1095
NdeII GATC 5 cut(s) 643, 798, 1135, 1165, 1269
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 8 cut(s) 70, 292, 516, 583, 806, 1148, 1201, 1223
NlaIV GGNNCC 4 cut(s) 307, 645, 848, 1167
NmuCI GTSAC 2 cut(s) 228, 449
NruI TCGCGA 1 cut(s) 120
NsbI TGCGCA 1 cut(s) 705
NspI RCATGY 1 cut(s) 70
PagI TCATGA 1 cut(s) 288
PcsI WCGNNNNNNNCGW 2 cut(s) 763, 1269
PfeI GAWTC 2 cut(s) 131, 1076
PkrI GCNGC 2 cut(s) 181, 238
PleI GAGTC 4 cut(s) 116, 287, 566, 718
PpsI GAGTC 4 cut(s) 116, 287, 566, 718
PshBI ATTAAT 1 cut(s) 710
Psp6I CCWGG 3 cut(s) 415, 661, 829
PspEI GGTNACC 1 cut(s) 761
PspGI CCWGG 3 cut(s) 415, 661, 829
PspN4I GGNNCC 4 cut(s) 307, 645, 848, 1167
PsrI GAACNNNNNNTAC 2 cut(s) 949, 981
PsuI RGATCY 2 cut(s) 643, 1165
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 5 cut(s) 18, 296, 517, 848, 883
RsaNI GTAC 5 cut(s) 17, 295, 516, 847, 882
SaqAI TTAA 4 cut(s) 353, 710, 753, 1299
SatI GCNGC 2 cut(s) 180, 237
Sau3AI GATC 5 cut(s) 643, 798, 1135, 1165, 1269
SchI GAGTC 4 cut(s) 116, 288, 567, 718
ScrFI CCNGG 3 cut(s) 417, 663, 831
SduI GDGCHC 1 cut(s) 166
SexAI ACCWGGT 1 cut(s) 829
SfaNI GCATC 3 cut(s) 351, 948, 986
SmlI CTYRAG 1 cut(s) 1250
SmoI CTYRAG 1 cut(s) 1250
Sse9I AATT 9 cut(s) 36, 208, 300, 354, 631, 707, 711, 779, 1027
SsiI CCGC 4 cut(s) 137, 259, 659, 1007
SspMI CTAG 4 cut(s) 150, 345, 476, 885
StyD4I CCNGG 3 cut(s) 415, 661, 829
TaaI ACNGT 6 cut(s) 461, 491, 767, 842, 881, 1316
TaiI ACGT 3 cut(s) 109, 288, 1266
TaqI TCGA 5 cut(s) 99, 585, 757, 835, 1272
TasI AATT 9 cut(s) 36, 208, 300, 354, 631, 707, 711, 779, 1027
TatI WGTACW 2 cut(s) 16, 515
TfiI GAWTC 2 cut(s) 131, 1076
Tru1I TTAA 4 cut(s) 353, 710, 753, 1299
Tru9I TTAA 4 cut(s) 353, 710, 753, 1299
TscAI CASTG 4 cut(s) 346, 371, 801, 1312
TseFI GTSAC 2 cut(s) 228, 449
TseI GCWGC 2 cut(s) 179, 236
Tsp45I GTSAC 2 cut(s) 228, 449
TspDTI ATGAA 8 cut(s) 16, 104, 501, 549, 568, 666, 1112, 1200
TspGWI ACGGA 2 cut(s) 656, 736
TspRI CASTG 4 cut(s) 346, 371, 801, 1312
VspI ATTAAT 1 cut(s) 710
XapI RAATTY 2 cut(s) 631, 779
XceI RCATGY 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 282
XspI CTAG 4 cut(s) 150, 345, 476, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.