Rroxscaffold_4G00296910

Aspartic proteinase CDR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013789
Physical Location & Seq
Reverse (-)
16925802 .. 16936814
11013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296910.1

Sequence Viewer

Length: 1194 bp
ATGTCACTGCTGAATTATGTGGATAGGTATAGAGACTTGTGTGACAAGCTTTATACCAAAGGGAAACAAGCATGGCAATTATCATCATCACAGCCACAATGTAGAGCTGCAGCTGTAAATGTTGTAGTTATCAAATTGAACAACGAGAGAAGCACCACGTTTGGGAGACACAAGGAGGAGTATCTAAATCGGGGGGAGTATCCGGATACATACTCCACACTCAACGCGCGTTGTGCTCTTTTTCTCCTTCGATCAAGCACACCAATATATGATCCTTCAAACTCACCAAGCTTTGTAGAAGTAAGGTGTAGCTCTCTAGACTGCAACATAACCGCAGATTTGCAATGCCCCGATCCTAATGGTATATGCAAGTACACGATTGCCCGCGCGTATGGAGATGGAAGATCTTCTGAAGGAGTCCTTGCTACAGAAACAATCACGCTCGAACATGATGGATCAAGTGGAAGCACTAGCTTACCAAATATGATTTTTGGGTGCGGTCACTCTAATGTAGACCCGAGATCAGGTTACAATCCACCCGGAATTGTGGGACTAAGCAGAGAACCTTCTTCCCCGGTAGGCCAAATTTCGTACCCACATGTTTCGTATTGCATATCAGCTAATAATGAAGGCAACCATAGCTCTGTCAAGTTCGGCTCTGTAGCAGTCATCACTGAGAATACTACACAAACCCCATTGTTAACTGGTCCACTTGGTTTTTACTATGTAAGCCTAGAAGGCATTAGTGTTGATGGTACAATGTTGGACATTCCGAAATCAGTGTTCAATATGAGGCCAGAAGGAGAAGGAGGGGTCTTCATGGATACAGGAACAGATCATACTCAGCTGGTACTGCCAGCATTTTATGCAGTCACCAGTAAAATAGAGGATATCTTGTTGGATTTCAAATCCTTACCAGATGTGGATGGCAGGTTCCCAGTGTGTTACAACGATACGAATTTTAGATTGGATTCGACACCAGAAATAGTATTCCTATTTACAGGACTGGAGTTTCCGTTGAGTGCCAATAACACTTGGGATCAGAACTCCCTGGGATACTATTGTTTGGCTATATTGCCAGCATTTCTGGGTCATTCTGTTCTTGGAATGTACCAGCAAAGGAACGTAAATGTTGGATATGACTTCAATAACAACATTCCATCTTTGAAGTATAGCGACAATAACTGCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

397

Amino Acids

43.62

Weight (kDa)

4.96

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 83 - 220 1.6e-22 Xylanase inhibitor N-terminal
TAXi_C PF14541 241 - 384 1.5e-21 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42980
malus_domestica MD01G1081400.v1.1 MD01G1081500.v1.1 MD01G1081600.v1.1 MD01G1081900.v1.1
prunus_persica Prupe.2G189100_v2.0.a1
pyrus_communis pycom07g14880
rosa_chinensis RchiOBHm_Chr1g0359581
rosa_laevigata RLG00000027860
rosa_multiflora Rmu_sc0002556.1_g000022
rosa_roxburghii Rroxscaffold_4G00296910
rosa_samantha Rh1AG285600 Rh1BG251700 Rh1BG251800 Rh1DG280900 Rh1DG281000
rosa_wichuraiana Rw1G025390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 921
AccI GTMKAC 1 cut(s) 513
AccII CGCG 4 cut(s) 227, 229, 387, 389
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 3 cut(s) 333, 385, 498
AclWI GGATC 4 cut(s) 266, 347, 463, 1047
AcsI RAATTY 2 cut(s) 585, 958
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 5 cut(s) 374, 593, 757, 852, 1112
AfiI CCNNNNNNNGG 2 cut(s) 162, 524
AflIII ACRYGT 1 cut(s) 598
AgsI TTSAA 6 cut(s) 139, 279, 787, 907, 1147, 1168
AjnI CCWGG 1 cut(s) 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 950, 982
AloI GAACNNNNNNTCC 2 cut(s) 917, 949
AluBI AGCT 9 cut(s) 49, 107, 113, 291, 312, 474, 620, 642, 847
AluI AGCT 9 cut(s) 49, 107, 113, 291, 312, 474, 620, 642, 847
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 2 cut(s) 27, 160
AlwI GGATC 4 cut(s) 266, 347, 463, 1047
Ama87I CYCGRG 1 cut(s) 517
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 2 cut(s) 580, 794
ApeKI GCWGC 2 cut(s) 107, 110
ApoI RAATTY 2 cut(s) 585, 958
Asp700I GAANNNNTTC 1 cut(s) 406
AspLEI GCGC 3 cut(s) 229, 389, 1190
AspS9I GGNCC 1 cut(s) 707
AsuC2I CCSGG 2 cut(s) 540, 575
AsuHPI GGTGA 2 cut(s) 276, 865
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 1 cut(s) 707
BaeI ACNNNNGTAYC 2 cut(s) 1048, 1081
BbsI GAAGAC 1 cut(s) 808
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 2 cut(s) 94, 122
BccI CCATC 5 cut(s) 392, 446, 746, 920, 1168
BciT130I CCWGG 1 cut(s) 1052
BciVI GTATCC 4 cut(s) 199, 210, 817, 1049
BcnI CCSGG 2 cut(s) 540, 575
BcoDI GTCTC 2 cut(s) 27, 160
BfaI CTAG 3 cut(s) 317, 471, 734
BfmI CTRYAG 3 cut(s) 108, 426, 660
BfuAI ACCTGC 1 cut(s) 921
BfuI GTATCC 4 cut(s) 199, 210, 817, 1049
BglI GCCNNNNNGGC 1 cut(s) 738
BglII AGATCT 1 cut(s) 404
BisI GCNGC 2 cut(s) 108, 111
BlsI GCNGC 2 cut(s) 109, 112
Bme1390I CCNGG 3 cut(s) 540, 575, 1052
Bme18I GGWCC 1 cut(s) 707
BmeT110I CYCGRG 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 707
BmiI GGNNCC 1 cut(s) 935
BmrFI CCNGG 3 cut(s) 540, 575, 1052
BmrI ACTGGG 1 cut(s) 932
BmuI ACTGGG 1 cut(s) 932
BpiI GAAGAC 1 cut(s) 808
BpmI CTGGAG 1 cut(s) 1028
BpuMI CCSGG 2 cut(s) 540, 575
BsaJI CCNNGG 3 cut(s) 573, 1050, 1051
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 1001, 1031
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 524
Bse1I ACTGG 4 cut(s) 709, 876, 938, 1011
Bse3DI GCAATG 1 cut(s) 350
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 1052
BseDI CCNNGG 3 cut(s) 573, 1050, 1051
BseGI GGATG 1 cut(s) 931
BseLI CCNNNNNNNGG 2 cut(s) 162, 524
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 2 cut(s) 666, 857
BseNI ACTGG 4 cut(s) 709, 876, 938, 1011
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 2 cut(s) 94, 122
Bsh1236I CGCG 4 cut(s) 227, 229, 387, 389
BshFI GGCC 2 cut(s) 582, 796
BsiHKAI GWGCWC 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 3 cut(s) 203, 540, 575
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 162, 524
BsmAI GTCTC 2 cut(s) 27, 160
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 2 cut(s) 582, 796
BsoBI CYCGRG 1 cut(s) 517
Bsp1286I GDGCHC 1 cut(s) 238
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 8 cut(s) 251, 271, 352, 404, 455, 521, 835, 1039
BspACI CCGC 3 cut(s) 333, 385, 498
BspANI GGCC 2 cut(s) 582, 796
BspCNI CTCAG 2 cut(s) 667, 856
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 4 cut(s) 227, 229, 387, 389
BspLI GGNNCC 1 cut(s) 935
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 921
BspPI GGATC 4 cut(s) 266, 347, 463, 1047
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 4 cut(s) 709, 876, 938, 1011
BssECI CCNNGG 3 cut(s) 573, 1050, 1051
BssMI GATC 8 cut(s) 251, 271, 352, 404, 455, 521, 835, 1039
Bst2UI CCWGG 1 cut(s) 1052
BstAPI GCANNNNNTGC 1 cut(s) 866
BstC8I GCNNGC 3 cut(s) 385, 858, 1080
BstDEI CTNAG 3 cut(s) 554, 675, 843
BstF5I GGATG 1 cut(s) 931
BstFNI CGCG 4 cut(s) 227, 229, 387, 389
BstHHI GCGC 3 cut(s) 229, 389, 1190
BstKTI GATC 8 cut(s) 254, 274, 355, 407, 458, 524, 838, 1042
BstMAI GTCTC 2 cut(s) 27, 160
BstMBI GATC 8 cut(s) 251, 271, 352, 404, 455, 521, 835, 1039
BstMWI GCNNNNNNNGC 5 cut(s) 233, 639, 738, 853, 866
BstNI CCWGG 1 cut(s) 1052
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 3 cut(s) 538, 573, 1050
BstSFI CTRYAG 3 cut(s) 108, 426, 660
BstUI CGCG 4 cut(s) 227, 229, 387, 389
BstV1I GCAGC 2 cut(s) 94, 122
BstV2I GAAGAC 1 cut(s) 808
BstX2I RGATCY 1 cut(s) 404
BstYI RGATCY 1 cut(s) 404
BsuI GTATCC 4 cut(s) 199, 210, 817, 1049
BsuRI GGCC 2 cut(s) 582, 796
BtsCI GGATG 1 cut(s) 931
BtsI GCAGTG 1 cut(s) 5
BtsIMutI CAGTG 4 cut(s) 5, 672, 786, 945
BveI ACCTGC 1 cut(s) 921
Cac8I GCNNGC 3 cut(s) 385, 858, 1080
CfoI GCGC 3 cut(s) 229, 389, 1190
Cfr13I GGNCC 1 cut(s) 707
Csp6I GTAC 5 cut(s) 373, 592, 756, 851, 1111
CviAII CATG 4 cut(s) 72, 449, 599, 820
CviQI GTAC 5 cut(s) 373, 592, 756, 851, 1111
DdeI CTNAG 3 cut(s) 554, 675, 843
DpnI GATC 8 cut(s) 253, 273, 354, 406, 457, 523, 837, 1041
DpnII GATC 8 cut(s) 251, 271, 352, 404, 455, 521, 835, 1039
Eco32I GATATC 1 cut(s) 892
Eco47I GGWCC 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 432
Eco88I CYCGRG 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 1050
EcoRV GATATC 1 cut(s) 892
FaeI CATG 4 cut(s) 75, 452, 602, 823
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 1 cut(s) 564
FatI CATG 4 cut(s) 71, 448, 598, 819
FauI CCCGC 1 cut(s) 392
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 108, 111
FokI GGATG 1 cut(s) 938
Fsp4HI GCNGC 2 cut(s) 108, 111
FspBI CTAG 3 cut(s) 317, 471, 734
GlaI GCGC 3 cut(s) 228, 388, 1189
GluI GCNGC 2 cut(s) 108, 111
GsuI CTGGAG 1 cut(s) 1028
HaeIII GGCC 2 cut(s) 582, 796
HapII CCGG 3 cut(s) 203, 540, 575
HhaI GCGC 3 cut(s) 229, 389, 1190
Hin1II CATG 4 cut(s) 75, 452, 602, 823
Hin6I GCGC 3 cut(s) 227, 387, 1188
HinP1I GCGC 3 cut(s) 227, 387, 1188
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HindIII AAGCTT 2 cut(s) 47, 289
HinfI GANTC 2 cut(s) 417, 971
HpaI GTTAAC 1 cut(s) 702
HpaII CCGG 3 cut(s) 203, 540, 575
HphI GGTGA 2 cut(s) 276, 865
Hpy166II GTNNAC 4 cut(s) 375, 514, 702, 710
Hpy188I TCNGA 3 cut(s) 412, 774, 1044
Hpy188III TCNNGA 2 cut(s) 203, 317
Hpy8I GTNNAC 4 cut(s) 375, 514, 702, 710
HpyAV CCTTC 8 cut(s) 257, 285, 407, 576, 623, 731, 794, 800
HpyCH4IV ACGT 2 cut(s) 158, 1125
HpyCH4V TGCA 6 cut(s) 110, 324, 343, 369, 612, 869
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 639, 738, 853, 866
HpyF3I CTNAG 3 cut(s) 554, 675, 843
HpySE526I ACGT 2 cut(s) 158, 1125
Hsp92II CATG 4 cut(s) 75, 452, 602, 823
HspAI GCGC 3 cut(s) 227, 387, 1188
Kpn2I TCCGGA 1 cut(s) 202
KspAI GTTAAC 1 cut(s) 702
Kzo9I GATC 8 cut(s) 251, 271, 352, 404, 455, 521, 835, 1039
Lsp1109I GCAGC 2 cut(s) 94, 122
MaeI CTAG 3 cut(s) 317, 471, 734
MaeII ACGT 2 cut(s) 158, 1125
MaeIII GTNAC 6 cut(s) 3, 41, 500, 527, 871, 944
MalI GATC 8 cut(s) 253, 273, 354, 406, 457, 523, 837, 1041
MboI GATC 8 cut(s) 251, 271, 352, 404, 455, 521, 835, 1039
MboII GAAGA 4 cut(s) 399, 414, 561, 808
MflI RGATCY 1 cut(s) 404
MhlI GDGCHC 1 cut(s) 238
MluCI AATT 6 cut(s) 13, 77, 134, 543, 585, 958
MlyI GAGTC 1 cut(s) 426
MmeI TCCRAC 3 cut(s) 744, 879, 1114
MnlI CCTC 4 cut(s) 169, 786, 803, 880
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 406
MseI TTAA 1 cut(s) 701
MslI CAYNNNNRTG 1 cut(s) 507
MspA1I CMGCKG 2 cut(s) 113, 847
MspI CCGG 3 cut(s) 203, 540, 575
MspR9I CCNGG 3 cut(s) 540, 575, 1052
MvaI CCWGG 1 cut(s) 1052
MvnI CGCG 4 cut(s) 227, 229, 387, 389
MwoI GCNNNNNNNGC 5 cut(s) 233, 639, 738, 853, 866
NciI CCSGG 2 cut(s) 540, 575
NdeII GATC 8 cut(s) 251, 271, 352, 404, 455, 521, 835, 1039
NlaIII CATG 4 cut(s) 75, 452, 602, 823
NlaIV GGNNCC 1 cut(s) 935
NmuCI GTSAC 4 cut(s) 3, 41, 500, 871
NspI RCATGY 1 cut(s) 602
PasI CCCWGGG 1 cut(s) 1051
PciI ACATGT 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 971
PkrI GCNGC 2 cut(s) 109, 112
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
PscI ACATGT 1 cut(s) 598
Psp6I CCWGG 1 cut(s) 1050
PspGI CCWGG 1 cut(s) 1050
PspN4I GGNNCC 1 cut(s) 935
PspPI GGNCC 1 cut(s) 707
PsrI GAACNNNNNNTAC 2 cut(s) 823, 855
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 404
PvuII CAGCTG 2 cut(s) 113, 847
RsaI GTAC 5 cut(s) 374, 593, 757, 852, 1112
RsaNI GTAC 5 cut(s) 373, 592, 756, 851, 1111
RseI CAYNNNNRTG 1 cut(s) 507
SaqAI TTAA 1 cut(s) 701
SatI GCNGC 2 cut(s) 108, 111
Sau3AI GATC 8 cut(s) 251, 271, 352, 404, 455, 521, 835, 1039
Sau96I GGNCC 1 cut(s) 707
SchI GAGTC 1 cut(s) 426
ScrFI CCNGG 3 cut(s) 540, 575, 1052
SduI GDGCHC 1 cut(s) 238
SfcI CTRYAG 3 cut(s) 108, 426, 660
SinI GGWCC 1 cut(s) 707
SmiMI CAYNNNNRTG 1 cut(s) 507
Sse9I AATT 6 cut(s) 13, 77, 134, 543, 585, 958
SsiI CCGC 3 cut(s) 333, 385, 498
SspMI CTAG 3 cut(s) 317, 471, 734
StyD4I CCNGG 3 cut(s) 538, 573, 1050
TaiI ACGT 2 cut(s) 161, 1128
TaqI TCGA 3 cut(s) 250, 444, 974
TasI AATT 6 cut(s) 13, 77, 134, 543, 585, 958
TatI WGTACW 1 cut(s) 372
TfiI GAWTC 1 cut(s) 971
Tru1I TTAA 1 cut(s) 701
Tru9I TTAA 1 cut(s) 701
TscAI CASTG 4 cut(s) 12, 679, 786, 945
TseFI GTSAC 4 cut(s) 3, 41, 500, 871
TseI GCWGC 2 cut(s) 107, 110
Tsp45I GTSAC 4 cut(s) 3, 41, 500, 871
TspDTI ATGAA 2 cut(s) 642, 808
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 4 cut(s) 12, 679, 786, 945
VpaK11BI GGWCC 1 cut(s) 707
XapI RAATTY 2 cut(s) 585, 958
XbaI TCTAGA 1 cut(s) 316
XceI RCATGY 1 cut(s) 602
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 406
XspI CTAG 3 cut(s) 317, 471, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.