MD01G1081500.v1.1

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013789
Physical Location & Seq
Forward (+)
18830797 .. 18831449
653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1081500.v1.1.491

Sequence Viewer

Length: 576 bp
ATGTCGAAACAAAAGCAGGCCATCACCCACCTGGAGTTGTGGGACTTAGGAGTTAGGAGAGAGCCTTCTTCACTTGTTGGTCAGATTTCGTACCCCCACCTTTCGTATTGCATTTCTTCCAAAAATAATGGCATCTACAATAACAGCAACGAGAAAATTGGGTTGCTACTTGCAGTGGTCACTGAAACTGAGGCAAAAACTCAAACACCTTGGTTGAGCGGTGAAAATGGTCTGTACTATCTAAACTTAGAGGGCATTAGCGTCGATGGCGCAATGCTCAATGTTTCGAGTTCAGTTTTTAATATGACCGCTGGTGGAGAGGGAGGTGTTTTCATGGACTCGGCAACAACTCATACTATGGTTGCATTTGGAGCTTTTAGTGAGCTGAGAAGCCGGTTTCAAGCTGATAAAACGCCGGAAATTGTGCTTCATTTCACGGGACTGGAGTTTCCGGTGAGTGCGGATAACACTTGGGCGGTGAATTCTCATGGACACTACTGCCTGGCTATTTTGCCAAGTTTTGCAGGTTTTACTATCTTTGGAATGTACCACTACCGTACCAGCACAGGAATGTGA

Protein Analysis

192

Amino Acids

20.92

Weight (kDa)

5.75

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_C PF14541 79 - 135 6.4e-08 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013789)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42980
malus_domestica MD01G1081400.v1.1 MD01G1081500.v1.1 MD01G1081600.v1.1 MD01G1081900.v1.1
prunus_persica Prupe.2G189100_v2.0.a1
pyrus_communis pycom07g14880
rosa_chinensis RchiOBHm_Chr1g0359581
rosa_laevigata RLG00000027860
rosa_multiflora Rmu_sc0002556.1_g000022
rosa_roxburghii Rroxscaffold_4G00296910
rosa_samantha Rh1AG285600 Rh1BG251700 Rh1BG251800 Rh1DG280900 Rh1DG281000
rosa_wichuraiana Rw1G025390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 515
AccBSI CCGCTC 1 cut(s) 219
AciI CCGC 4 cut(s) 219, 309, 461, 476
AcsI RAATTY 1 cut(s) 481
AfaI GTAC 4 cut(s) 92, 236, 548, 559
AgsI TTSAA 1 cut(s) 401
AjnI CCWGG 2 cut(s) 30, 501
AluBI AGCT 3 cut(s) 374, 385, 404
AluI AGCT 3 cut(s) 374, 385, 404
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 481
AspLEI GCGC 1 cut(s) 272
AsuHPI GGTGA 4 cut(s) 16, 233, 466, 490
BaeI ACNNNNGTAYC 2 cut(s) 541, 574
BccI CCATC 2 cut(s) 29, 260
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 2 cut(s) 32, 503
BfuAI ACCTGC 1 cut(s) 515
Bme1390I CCNGG 2 cut(s) 32, 503
BmrFI CCNGG 2 cut(s) 32, 503
BmsI GCATC 1 cut(s) 141
BpmI CTGGAG 2 cut(s) 53, 464
BsaJI CCNNGG 1 cut(s) 209
BsaWI WCCGGW 1 cut(s) 451
Bse118I RCCGGY 1 cut(s) 393
Bse1I ACTGG 1 cut(s) 447
Bse3DI GCAATG 1 cut(s) 279
BseBI CCWGG 2 cut(s) 32, 503
BseDI CCNNGG 1 cut(s) 209
BseMI GCAATG 1 cut(s) 279
BseMII CTCAG 2 cut(s) 180, 377
BseNI ACTGG 1 cut(s) 447
BshFI GGCC 1 cut(s) 20
BsiSI CCGG 3 cut(s) 394, 416, 452
BslFI GGGAC 2 cut(s) 56, 453
BsmFI GGGAC 2 cut(s) 56, 453
BsnI GGCC 1 cut(s) 20
BspACI CCGC 4 cut(s) 219, 309, 461, 476
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 2 cut(s) 181, 378
BspMI ACCTGC 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 279
BsrFI RCCGGY 1 cut(s) 393
BsrI ACTGG 1 cut(s) 447
BssAI RCCGGY 1 cut(s) 393
BssECI CCNNGG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 32, 503
Bst4CI ACNGT 1 cut(s) 557
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 4 cut(s) 46, 189, 247, 386
BstHHI GCGC 1 cut(s) 272
BstMWI GCNNNNNNNGC 2 cut(s) 267, 371
BstNI CCWGG 2 cut(s) 32, 503
BstSCI CCNGG 2 cut(s) 30, 501
BsuRI GGCC 1 cut(s) 20
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 180, 180
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 1 cut(s) 18
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 1 cut(s) 393
CseI GACGC 1 cut(s) 250
Csp6I GTAC 4 cut(s) 91, 235, 547, 558
CviAII CATG 2 cut(s) 334, 488
CviJI RGCY 7 cut(s) 20, 64, 374, 385, 393, 404, 506
CviKI_1 RGCY 7 cut(s) 20, 64, 374, 385, 393, 404, 506
CviQI GTAC 4 cut(s) 91, 235, 547, 558
DdeI CTNAG 4 cut(s) 46, 189, 247, 386
Eco130I CCWWGG 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 481
EcoRII CCWGG 2 cut(s) 30, 501
EcoT14I CCWWGG 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 2 cut(s) 337, 491
FaiI YATR 5 cut(s) 305, 335, 354, 359, 489
FaqI GGGAC 2 cut(s) 56, 453
FatI CATG 2 cut(s) 333, 487
GlaI GCGC 1 cut(s) 271
GsuI CTGGAG 2 cut(s) 53, 464
HaeIII GGCC 1 cut(s) 20
HapII CCGG 3 cut(s) 394, 416, 452
HgaI GACGC 1 cut(s) 250
HhaI GCGC 1 cut(s) 272
Hin1II CATG 2 cut(s) 337, 491
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HinfI GANTC 1 cut(s) 338
HpaII CCGG 3 cut(s) 394, 416, 452
HphI GGTGA 4 cut(s) 16, 233, 466, 490
Hpy188I TCNGA 1 cut(s) 84
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4V TGCA 4 cut(s) 111, 173, 365, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 267, 371
HpyF3I CTNAG 4 cut(s) 46, 189, 247, 386
Hsp92II CATG 2 cut(s) 337, 491
HspAI GCGC 1 cut(s) 270
LmnI GCTCC 1 cut(s) 371
LweI GCATC 1 cut(s) 141
MaeIII GTNAC 1 cut(s) 178
MbiI CCGCTC 1 cut(s) 219
MboII GAAGA 2 cut(s) 60, 108
MluCI AATT 3 cut(s) 156, 420, 481
MlyI GAGTC 1 cut(s) 332
MnlI CCTC 4 cut(s) 184, 244, 313, 317
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 3 cut(s) 394, 416, 452
MspR9I CCNGG 2 cut(s) 32, 503
MvaI CCWGG 2 cut(s) 32, 503
MwoI GCNNNNNNNGC 2 cut(s) 267, 371
NlaIII CATG 2 cut(s) 337, 491
NmeAIII GCCGAG 1 cut(s) 320
NmuCI GTSAC 1 cut(s) 178
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Psp6I CCWGG 2 cut(s) 30, 501
PspGI CCWGG 2 cut(s) 30, 501
RsaI GTAC 4 cut(s) 92, 236, 548, 559
RsaNI GTAC 4 cut(s) 91, 235, 547, 558
RseI CAYNNNNRTG 1 cut(s) 569
SaqAI TTAA 1 cut(s) 300
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 32, 503
SetI ASST 8 cut(s) 33, 102, 211, 328, 376, 387, 406, 529
SfaNI GCATC 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 569
Sse9I AATT 3 cut(s) 156, 420, 481
SsiI CCGC 4 cut(s) 219, 309, 461, 476
StyD4I CCNGG 2 cut(s) 30, 501
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 1 cut(s) 557
TaqI TCGA 3 cut(s) 5, 264, 287
TasI AATT 3 cut(s) 156, 420, 481
TatI WGTACW 1 cut(s) 234
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 180, 187
TseFI GTSAC 1 cut(s) 178
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 322, 419
TspRI CASTG 2 cut(s) 180, 187
XapI RAATTY 1 cut(s) 481
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.