AT2G43050

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011630
Physical Location & Seq
Forward (+)
17902370 .. 17904443
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G43050.1

Sequence Viewer

Length: 1557 bp
ATGGCTTCATCATCATCAATATCAAATCACAAGATTCCAAACACACTAATGTTTTTGGTGATCGTAAACTTTTTGTACCTAATTCAAACTAATTCAGCTGTCAGTATCAGTTCCAATTCCAACTCCCACTTCTCAAGATTCTCGAGACATCGAAGCTCACCATCATCAAAAACCAAACAAGGGTTTCTTGCAACGGTCCAAGAGAGTATGAACCATGCTCTCTTGGCTCGTTCTCTTGCTTTCAACCTCACTCTTTCTCATCGAACCGTACAAACCCACACGTTCGATCCCATCCACGACTGCCTCGAGCTGCTCGACGACACACTTGACATGTTATCTCGCATCCATGCGGATAATGATGAAGAAGATGTCCATACATGGCTAAGCGCAGCACTCACGAACCAAGACACTTGTGAGCAGAGCCTTCAAGAAAAATCCGAGTCATACAAACACGGACTTGCGATGGATTTCGTCGCAAGAAACCTAACCGGTTTGTTGACTAGCTCGCTTGACTTGTTCGTATCCGTCAAGTCAAAACACCGGAAACTCTTATCGAAACAAGAGTATTTTCCGACGTTCGTTCCTTCGTCGGAGCAACGGAGGCTTCTAGAAGCTCCGGTGGAGGAGCTGAATGTTGATGCGGTGGTGGCTCCTGACGGAAGTGGGACACACAAAACCATAGGAGAAGCCTTGTTGTCTACGTCATTAGCGAGTAGTGGGGGCAGGACCAAAATATACCTAAAAGCCGGGACATATCATGAGAACATCAACATTCCGACGAAGCAGAAGAACGTTATGTTAGTTGGTGACGGGAAGGGTAAAACAGTCATTGTAGGTAGCAGAAGTAACAGAGGCGGCTGGACTACTTACAAAACTGCAACCGTCGCCGCTATGGGGGAAGGGTTCATAGCGCGGGACATGACATTCGTGAACAACGCCGGACCCAAATCGGAGCAAGCGGTGGCGCTCCGTGTCGGAGCAGATAAATCCGTCGTGCATCGATGCTCAGTGGAAGGATACCAAGATTCACTCTACACACACTCCAAACGACAATTCTATAGAGAGACGGATATCACCGGCACCGTCGATTTCATATTCGGAAACTCCGCCGTCGTATTTCAATCCTGCAACATCGCCGCACGGAAACCTTTACCGGGTCAGAGAAACTTCGTGACGGCGCAAGGACGGAGCAATCCGGGTCAAAACACCGGAATCGCGATTCAAAACTGCAGGATCACGGCGGAATCGATGACTTATCTTGGTCGGCCGTGGAAGGAGTATTCGAGGACGGTGGTGATGCAATCATTTATCGGCGGGTCGATTCATCCGTCGGGTTGGTCTCCTTGGTCGGGTGGTTTCGGTCTCAAATCTCTGTTTTACGGAGAATATGGGAATTCGGGTCCTGGATCATCGGTTTCGGGTCGGGTTAAATGGAGTGGGTGTCATCCGTCTTTGACGGTGACGGAAGCGGAAAAATTCACTGTGGCGAGTTTTATTGACGGGAATATATGGTTGCCGTCAACGGGCGTTAGTTTCGACCCAGGACTTGTGAATTGA

Protein Analysis

518

Amino Acids

56.59

Weight (kDa)

8.85

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 48 - 155 2.8e-16 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 213 - 502 7.3e-134 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1079
AccI GTMKAC 1 cut(s) 698
AccII CGCG 2 cut(s) 913, 1217
AclI AACGTT 1 cut(s) 792
AclWI GGATC 3 cut(s) 281, 1241, 1414
AcoI YGGCCR 1 cut(s) 1265
AcsI RAATTY 2 cut(s) 1393, 1475
AfaI GTAC 2 cut(s) 77, 270
AfiI CCNNNNNNNGG 5 cut(s) 180, 894, 1154, 1349, 1523
AflIII ACRYGT 2 cut(s) 279, 330
AgeI ACCGGT 1 cut(s) 488
AgsI TTSAA 5 cut(s) 86, 244, 428, 1121, 1223
AjnI CCWGG 2 cut(s) 1402, 1540
AjuI GAANNNNNNNTTGG 4 cut(s) 113, 145, 1037, 1069
AluBI AGCT 6 cut(s) 98, 156, 310, 504, 614, 628
AluI AGCT 6 cut(s) 98, 156, 310, 504, 614, 628
Alw26I GTCTC 4 cut(s) 139, 1058, 1344, 1367
AlwI GGATC 3 cut(s) 281, 1241, 1414
Ama87I CYCGRG 2 cut(s) 142, 305
AoxI GGCC 1 cut(s) 1265
ApeKI GCWGC 2 cut(s) 310, 389
ApoI RAATTY 2 cut(s) 1393, 1475
ArsI GACNNNNNNTTYG 2 cut(s) 908, 940
AsiGI ACCGGT 1 cut(s) 488
AspLEI GCGC 4 cut(s) 389, 913, 967, 1180
AspS9I GGNCC 4 cut(s) 196, 726, 941, 1400
AsuC2I CCSGG 3 cut(s) 748, 1155, 1197
AsuHPI GGTGA 6 cut(s) 70, 150, 818, 1066, 1306, 1471
AvaI CYCGRG 2 cut(s) 142, 305
AvaII GGWCC 4 cut(s) 196, 726, 941, 1400
BanI GGYRCC 1 cut(s) 1079
BbvI GCAGC 2 cut(s) 297, 401
BccI CCATC 3 cut(s) 169, 299, 457
BceAI ACGGC 5 cut(s) 1094, 1191, 1252, 1254, 1501
BciT130I CCWGG 2 cut(s) 1404, 1542
BciVI GTATCC 2 cut(s) 532, 1010
BcnI CCSGG 3 cut(s) 748, 1155, 1197
BcoDI GTCTC 4 cut(s) 139, 1058, 1344, 1367
BfaI CTAG 2 cut(s) 501, 608
BfmI CTRYAG 2 cut(s) 1057, 1228
BfoI RGCGCY 1 cut(s) 968
BfuI GTATCC 2 cut(s) 532, 1010
BisI GCNGC 5 cut(s) 311, 390, 856, 888, 1137
BlpI GCTNAGC 1 cut(s) 383
BlsI GCNGC 5 cut(s) 312, 391, 857, 889, 1138
Bme1390I CCNGG 5 cut(s) 748, 1155, 1197, 1404, 1542
Bme18I GGWCC 4 cut(s) 196, 726, 941, 1400
BmeT110I CYCGRG 2 cut(s) 142, 305
BmgT120I GGNCC 4 cut(s) 196, 726, 941, 1400
BmiI GGNNCC 4 cut(s) 651, 943, 1081, 1401
BmrFI CCNGG 5 cut(s) 748, 1155, 1197, 1404, 1542
BmsI GCATC 5 cut(s) 351, 628, 992, 1006, 1287
Bpu1102I GCTNAGC 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 118
BpuMI CCSGG 3 cut(s) 748, 1155, 1197
Bsa29I ATCGAT 2 cut(s) 1000, 1247
BsaI GGTCTC 2 cut(s) 1344, 1367
BsaJI CCNNGG 3 cut(s) 1268, 1343, 1540
BsaWI WCCGGW 4 cut(s) 488, 540, 616, 1208
BsaXI ACNNNNNCTCC 4 cut(s) 1025, 1055, 1426, 1456
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 894, 1154, 1349, 1523
Bse118I RCCGGY 2 cut(s) 488, 1076
BseBI CCWGG 2 cut(s) 1404, 1542
BseCI ATCGAT 2 cut(s) 1000, 1247
BseDI CCNNGG 3 cut(s) 1268, 1343, 1540
BseGI GGATG 4 cut(s) 291, 342, 1324, 1444
BseLI CCNNNNNNNGG 5 cut(s) 180, 894, 1154, 1349, 1523
BseMII CTCAG 1 cut(s) 1020
BseRI GAGGAG 1 cut(s) 638
BseX3I CGGCCG 1 cut(s) 1265
BseXI GCAGC 2 cut(s) 297, 401
Bsh1236I CGCG 2 cut(s) 913, 1217
Bsh1285I CGRYCG 1 cut(s) 1268
BshFI GGCC 1 cut(s) 1267
BshNI GGYRCC 1 cut(s) 1079
BshTI ACCGGT 1 cut(s) 488
BshVI ATCGAT 2 cut(s) 1000, 1247
BsiEI CGRYCG 1 cut(s) 1268
BsiHKCI CYCGRG 2 cut(s) 142, 305
BsiSI CCGG 9 cut(s) 489, 541, 617, 747, 939, 1077, 1154, 1196, 1209
BslFI GGGAC 3 cut(s) 679, 763, 929
BslI CCNNNNNNNGG 5 cut(s) 180, 894, 1154, 1349, 1523
BsmAI GTCTC 4 cut(s) 139, 1058, 1344, 1367
BsmBI CGTCTC 1 cut(s) 1058
BsmFI GGGAC 3 cut(s) 679, 763, 929
BsnI GGCC 1 cut(s) 1267
Bso31I GGTCTC 2 cut(s) 1344, 1367
BsoBI CYCGRG 2 cut(s) 142, 305
Bsp143I GATC 4 cut(s) 60, 286, 1233, 1406
Bsp1720I GCTNAGC 1 cut(s) 383
Bsp68I TCGCGA 1 cut(s) 1217
BspANI GGCC 1 cut(s) 1267
BspCNI CTCAG 1 cut(s) 1019
BspDI ATCGAT 2 cut(s) 1000, 1247
BspFNI CGCG 2 cut(s) 913, 1217
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 4 cut(s) 651, 943, 1081, 1401
BspMAI CTGCAG 1 cut(s) 1232
BspPI GGATC 3 cut(s) 281, 1241, 1414
BspT107I GGYRCC 1 cut(s) 1079
BspTNI GGTCTC 2 cut(s) 1344, 1367
BsrFI RCCGGY 2 cut(s) 488, 1076
BssAI RCCGGY 2 cut(s) 488, 1076
BssECI CCNNGG 3 cut(s) 1268, 1343, 1540
BssMI GATC 4 cut(s) 60, 286, 1233, 1406
BssT1I CCWWGG 1 cut(s) 1343
Bst2UI CCWGG 2 cut(s) 1404, 1542
Bst4CI ACNGT 8 cut(s) 196, 268, 826, 883, 1084, 1291, 1459, 1483
BstC8I GCNNGC 2 cut(s) 506, 957
BstDEI CTNAG 2 cut(s) 383, 1006
BstDSI CCRYGG 1 cut(s) 1268
BstF5I GGATG 4 cut(s) 291, 342, 1324, 1444
BstFNI CGCG 2 cut(s) 913, 1217
BstH2I RGCGCY 1 cut(s) 968
BstHHI GCGC 4 cut(s) 389, 913, 967, 1180
BstKTI GATC 4 cut(s) 63, 289, 1236, 1409
BstMAI GTCTC 4 cut(s) 139, 1058, 1344, 1367
BstMBI GATC 4 cut(s) 60, 286, 1233, 1406
BstMCI CGRYCG 1 cut(s) 1268
BstMWI GCNNNNNNNGC 4 cut(s) 224, 601, 647, 884
BstNI CCWGG 2 cut(s) 1404, 1542
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 5 cut(s) 746, 1153, 1195, 1402, 1540
BstSFI CTRYAG 2 cut(s) 1057, 1228
BstUI CGCG 2 cut(s) 913, 1217
BstV1I GCAGC 2 cut(s) 297, 401
BstZI CGGCCG 1 cut(s) 1265
Bsu15I ATCGAT 2 cut(s) 1000, 1247
BsuI GTATCC 2 cut(s) 532, 1010
BsuRI GGCC 1 cut(s) 1267
BsuTUI ATCGAT 2 cut(s) 1000, 1247
BtgI CCRYGG 1 cut(s) 1268
BtgZI GCGATG 2 cut(s) 476, 1117
BtsCI GGATG 4 cut(s) 291, 342, 1324, 1444
BtsIMutI CAGTG 2 cut(s) 1014, 1479
BtuMI TCGCGA 1 cut(s) 1217
Cac8I GCNNGC 2 cut(s) 506, 957
CciI TCATGA 1 cut(s) 757
CfoI GCGC 4 cut(s) 389, 913, 967, 1180
Cfr10I RCCGGY 2 cut(s) 488, 1076
Cfr13I GGNCC 4 cut(s) 196, 726, 941, 1400
ClaI ATCGAT 2 cut(s) 1000, 1247
Csp6I GTAC 2 cut(s) 76, 269
CspAI ACCGGT 1 cut(s) 488
CviAII CATG 6 cut(s) 215, 331, 347, 378, 758, 919
CviQI GTAC 2 cut(s) 76, 269
DdeI CTNAG 2 cut(s) 383, 1006
DpnI GATC 4 cut(s) 62, 288, 1235, 1408
DpnII GATC 4 cut(s) 60, 286, 1233, 1406
EaeI YGGCCR 1 cut(s) 1265
EagI CGGCCG 1 cut(s) 1265
EciI GGCGGA 2 cut(s) 1096, 1256
EclXI CGGCCG 1 cut(s) 1265
Eco130I CCWWGG 1 cut(s) 1343
Eco31I GGTCTC 2 cut(s) 1344, 1367
Eco32I GATATC 1 cut(s) 1072
Eco47I GGWCC 4 cut(s) 196, 726, 941, 1400
Eco52I CGGCCG 1 cut(s) 1265
Eco88I CYCGRG 2 cut(s) 142, 305
EcoO109I RGGNCCY 1 cut(s) 1400
EcoRI GAATTC 1 cut(s) 1393
EcoRII CCWGG 2 cut(s) 1402, 1540
EcoRV GATATC 1 cut(s) 1072
EcoT14I CCWWGG 1 cut(s) 1343
ErhI CCWWGG 1 cut(s) 1343
Esp3I CGTCTC 1 cut(s) 1058
FaeI CATG 6 cut(s) 218, 334, 350, 381, 761, 922
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FaqI GGGAC 3 cut(s) 679, 763, 929
FatI CATG 6 cut(s) 214, 330, 346, 377, 757, 918
FauI CCCGC 2 cut(s) 906, 1307
FblI GTMKAC 1 cut(s) 698
Fnu4HI GCNGC 5 cut(s) 311, 390, 856, 888, 1137
FokI GGATG 4 cut(s) 278, 329, 1311, 1431
Fsp4HI GCNGC 5 cut(s) 311, 390, 856, 888, 1137
FspBI CTAG 2 cut(s) 501, 608
GlaI GCGC 4 cut(s) 388, 912, 966, 1179
GluI GCNGC 5 cut(s) 311, 390, 856, 888, 1137
HaeII RGCGCY 1 cut(s) 968
HaeIII GGCC 1 cut(s) 1267
HapII CCGG 9 cut(s) 489, 541, 617, 747, 939, 1077, 1154, 1196, 1209
HhaI GCGC 4 cut(s) 389, 913, 967, 1180
Hin1II CATG 6 cut(s) 218, 334, 350, 381, 761, 922
Hin6I GCGC 4 cut(s) 387, 911, 965, 1178
HinP1I GCGC 4 cut(s) 387, 911, 965, 1178
HincII GTYRAC 2 cut(s) 498, 1521
HindII GTYRAC 2 cut(s) 498, 1521
HinfI GANTC 8 cut(s) 34, 138, 440, 1025, 1212, 1219, 1244, 1321
HpaII CCGG 9 cut(s) 489, 541, 617, 747, 939, 1077, 1154, 1196, 1209
HphI GGTGA 6 cut(s) 70, 150, 818, 1066, 1306, 1471
Hpy166II GTNNAC 5 cut(s) 67, 498, 699, 931, 1521
Hpy188I TCNGA 8 cut(s) 439, 573, 592, 777, 952, 977, 1100, 1161
Hpy8I GTNNAC 5 cut(s) 67, 498, 699, 931, 1521
HpyAV CCTTC 6 cut(s) 434, 594, 808, 893, 1007, 1267
HpyCH4III ACNGT 8 cut(s) 196, 268, 826, 883, 1084, 1291, 1459, 1483
HpyCH4IV ACGT 4 cut(s) 281, 575, 701, 792
HpyCH4V TGCA 6 cut(s) 191, 878, 997, 1128, 1230, 1300
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 601, 647, 884
HpyF3I CTNAG 2 cut(s) 383, 1006
HpySE526I ACGT 4 cut(s) 281, 575, 701, 792
Hsp92II CATG 6 cut(s) 218, 334, 350, 381, 761, 922
HspAI GCGC 4 cut(s) 387, 911, 965, 1178
Kzo9I GATC 4 cut(s) 60, 286, 1233, 1406
LmnI GCTCC 8 cut(s) 592, 619, 625, 655, 952, 972, 977, 1188
Lsp1109I GCAGC 2 cut(s) 297, 401
LweI GCATC 5 cut(s) 351, 628, 992, 1006, 1287
MaeI CTAG 2 cut(s) 501, 608
MaeII ACGT 4 cut(s) 281, 575, 701, 792
MaeIII GTNAC 4 cut(s) 806, 845, 1171, 1459
MalI GATC 4 cut(s) 62, 288, 1235, 1408
MboI GATC 4 cut(s) 60, 286, 1233, 1406
MboII GAAGA 3 cut(s) 374, 377, 799
MluCI AATT 7 cut(s) 81, 91, 115, 1052, 1393, 1475, 1552
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 5 cut(s) 144, 570, 596, 800, 955
MnlI CCTC 6 cut(s) 257, 314, 594, 616, 845, 1278
MseI TTAA 1 cut(s) 1428
MslI CAYNNNNRTG 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 9 cut(s) 489, 541, 617, 747, 939, 1077, 1154, 1196, 1209
MspR9I CCNGG 5 cut(s) 748, 1155, 1197, 1404, 1542
MvaI CCWGG 2 cut(s) 1404, 1542
MvnI CGCG 2 cut(s) 913, 1217
MwoI GCNNNNNNNGC 4 cut(s) 224, 601, 647, 884
NciI CCSGG 3 cut(s) 748, 1155, 1197
NdeII GATC 4 cut(s) 60, 286, 1233, 1406
NlaIII CATG 6 cut(s) 218, 334, 350, 381, 761, 922
NlaIV GGNNCC 4 cut(s) 651, 943, 1081, 1401
NmuCI GTSAC 3 cut(s) 806, 1171, 1459
NruI TCGCGA 1 cut(s) 1217
NspI RCATGY 1 cut(s) 334
PaeR7I CTCGAG 2 cut(s) 142, 305
PagI TCATGA 1 cut(s) 757
PciI ACATGT 1 cut(s) 330
PcsI WCGNNNNNNNCGW 5 cut(s) 303, 312, 707, 1244, 1325
PfeI GAWTC 7 cut(s) 34, 138, 1025, 1212, 1219, 1244, 1321
PfoI TCCNGGA 1 cut(s) 1402
PinAI ACCGGT 1 cut(s) 488
PkrI GCNGC 5 cut(s) 312, 391, 857, 889, 1138
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 1400
PscI ACATGT 1 cut(s) 330
Psp1406I AACGTT 1 cut(s) 792
Psp5II RGGWCCY 1 cut(s) 1400
Psp6I CCWGG 2 cut(s) 1402, 1540
PspGI CCWGG 2 cut(s) 1402, 1540
PspN4I GGNNCC 4 cut(s) 651, 943, 1081, 1401
PspPI GGNCC 4 cut(s) 196, 726, 941, 1400
PspPPI RGGWCCY 1 cut(s) 1400
PspXI VCTCGAGB 1 cut(s) 305
PstI CTGCAG 1 cut(s) 1232
PvuII CAGCTG 1 cut(s) 98
RruI TCGCGA 1 cut(s) 1217
RsaI GTAC 2 cut(s) 77, 270
RsaNI GTAC 2 cut(s) 76, 269
RseI CAYNNNNRTG 1 cut(s) 47
SaqAI TTAA 1 cut(s) 1428
SatI GCNGC 5 cut(s) 311, 390, 856, 888, 1137
Sau3AI GATC 4 cut(s) 60, 286, 1233, 1406
Sau96I GGNCC 4 cut(s) 196, 726, 941, 1400
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 5 cut(s) 748, 1155, 1197, 1404, 1542
SfaNI GCATC 5 cut(s) 351, 628, 992, 1006, 1287
SfcI CTRYAG 2 cut(s) 1057, 1228
Sfr274I CTCGAG 2 cut(s) 142, 305
SinI GGWCC 4 cut(s) 196, 726, 941, 1400
SlaI CTCGAG 2 cut(s) 142, 305
SmiMI CAYNNNNRTG 1 cut(s) 47
SmlI CTYRAG 3 cut(s) 133, 142, 305
SmoI CTYRAG 3 cut(s) 133, 142, 305
Sse9I AATT 7 cut(s) 81, 91, 115, 1052, 1393, 1475, 1552
SspMI CTAG 2 cut(s) 501, 608
StyD4I CCNGG 5 cut(s) 746, 1153, 1195, 1402, 1540
StyI CCWWGG 1 cut(s) 1343
TaaI ACNGT 8 cut(s) 196, 268, 826, 883, 1084, 1291, 1459, 1483
TaiI ACGT 4 cut(s) 284, 578, 704, 795
TaqII GACCGA 1 cut(s) 1349
TasI AATT 7 cut(s) 81, 91, 115, 1052, 1393, 1475, 1552
TauI GCSGC 3 cut(s) 858, 890, 1139
TfiI GAWTC 7 cut(s) 34, 138, 1025, 1212, 1219, 1244, 1321
Tru1I TTAA 1 cut(s) 1428
Tru9I TTAA 1 cut(s) 1428
TscAI CASTG 2 cut(s) 1014, 1486
TseFI GTSAC 3 cut(s) 806, 1171, 1459
TseI GCWGC 2 cut(s) 310, 389
Tsp45I GTSAC 3 cut(s) 806, 1171, 1459
TspDTI ATGAA 5 cut(s) 224, 375, 895, 1081, 1313
TspRI CASTG 2 cut(s) 1014, 1486
VpaK11BI GGWCC 4 cut(s) 196, 726, 941, 1400
XapI RAATTY 2 cut(s) 1393, 1475
XbaI TCTAGA 1 cut(s) 607
XceI RCATGY 1 cut(s) 334
XhoI CTCGAG 2 cut(s) 142, 305
XmiI GTMKAC 1 cut(s) 698
XspI CTAG 2 cut(s) 501, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.