Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0011630

Physical Location & Seq

Genomic Coordinates Reverse (-)

40219683 .. 40222438

Gene Structure 2756 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG158100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1785 bp

ATGTTCAAAATTATGTTCTTGGCGGTGGAAATTTTATTCTTCTCAAGCATATTATTAGCAAAAGCTTCTCCTTCACAATCCCATTTCTCCAAATTTTCTAGATCATCCGGAGGACTTTCCCATAAAGAAGCCATTTTATCTCTCTGCGCACATACCCCTCACCGAGCAACTTGTGAATCCACTCTCCTCTCGTCCTCCTCATCATCATCATCTACAAGAGGCTCTGATCATCTCCCTCATACCCTAGAAGGTGCCTTTGTCCAGTCGGTCCATTCCACCGTTGCGAAAGCTCAGCACGCTCGAGCTCTCGCCTATAATCTCACTATTTCCCACCGCCAAAATGCCGATCATGATGTTCACTTCCCTGGTCGCGGTGTGAACGACTGCCTTGAGCTTCTCGATGATGCTATAGATATGCTCGACAATGTCATGATGACATCGTTGTCAAAACCTAACGATGACGACGTTCACACCTGGCTTAGTGCAGCTCTTACTAACCAAGAAACTTGTCTAGAGAGCCTCGAGAAAGACAAGTTCAAGACAGAGAAAAACATTATGGATGTCACGGCTCAAAATCTGAGCCAATACATAAGCAACTCCCTAGCACTTTTTGTGCTTACCATGGGCAGTAGCAGTGTCTCCTTGGCTAGTAGTTCGGAGCATTTACCTCGAGACACTGAGTCAAAGTCAAAGGAAGCTAAAAATGCAAACCCTAATAGAAGACTATTGGCATCGGACAGCAGTTTTCCGACGTGGGTCCCGGCCTCAGAGAGGAAGCTTCTAGAAGCTTCTGTTGGGGAGATAGAAGCAGATGCTGTGGTGGCTCAGGATGGGAGTGGGACGCACAAGAGCATTGGTGAGGCAGTGAAGGGGTTGCTGCTAATTGGATCCTTGGAGAGTAGTGGTACTAGTACTGGGGGGAGATCTGTGATTTACGTAAAAGCTGGGACATACCACGAGTACATAAACATACCCACGAAGCAAAAGAACGTGATGTTGATTGGTGATGGAGTGGGGAAAACGATCATTGTGGGCAACAGGAACTCAGACGATGGCTGGACAACTTATAAGTCTGCTACCGTGGCTGCTATGGGTGATGGGTTCATCGCTCGTGATATAACATTTGTGAACGATGCCGGCCCTTCCAAGCACCAAGCAGTCGCACTTCGAGTCGGTGCCGACAAATCAGTCGTCCACCGATGTTCCATCATAGGCTACCAGGACACCCTCTACACTCACTCCAAGCGACAGTTCTACCGAGAAACCGACATCTATGGAACCGTCGACTTCATCTTCGGCAACTCAGCCGTGGTTTTCCAAACTTGCAACATCCACCCCCGAAAACCGCCCTCCAGCGGGCTCAAGAACTTTGTCACAGCTCAAGGCCGAAGCAGCCCTAATCAGAACACAGGTATTTCAATCCACAACTGTAAGATAGCGGCGGCATCAGACCTCTTCCCGGTGAGATCCAGCTACGAGACGTATCTTGGGAGACCGTGGCAGCAGTATTCGAGAACGATCATCATGCAGTCTTTCATGGATGACTCGATAAATCGAGCAGGGTGGTCTCCTTGGTCTGGTGGGTCTGCACTTAACACATTGTACTATGGAGAGTACATGAATTCTGGGTCTGGGGCATCAACCTCAGGAAGGGTTAAATGGGGTGGATATCATGCATCACTTACGTCCACTCAAGCAGAGGCATTCACAGTTGGGGGTTTCATATCTGGGAATCTTTGGCTGCCTTCCACTGGGGTGTCTTTTAGTTCTGGACTAATTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

594

Amino Acids

64.1

Weight (kDa)

7.07

Isoelectric Point (pI)

35.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	44 - 203	5.5e-26	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	271 - 578	5.3e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43050 AT3G59010
fragaria_vesca	FvH4_4g12140
malus_domestica	MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica	Prupe.1G178400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0409791
rosa_laevigata	RLG00000008522
rosa_multiflora	Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354340
rosa_rugosa	Rorug04G0096600
rosa_samantha	Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana	Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1068
AasI	GACNNNNNNGTC	2 cut(s)	442, 1187
Acc16I	TGCGCA	1 cut(s)	148
AccB1I	GGYRCC	2 cut(s)	251, 1175
AccI	GTMKAC	1 cut(s)	1284
AccII	CGCG	1 cut(s)	372
AccIII	TCCGGA	1 cut(s)	107
AciI	CCGC	7 cut(s)	23, 334, 372, 1346, 1356, 1439, 1442
AclWI	GGATC	3 cut(s)	882, 895, 1461
AcsI	RAATTY	3 cut(s)	30, 92, 1621
AfaI	GTAC	5 cut(s)	907, 913, 962, 1604, 1616
AfiI	CCNNNNNNNGG	8 cut(s)	371, 771, 1355, 1356, 1459, 1577, 1650, 1751
AgsI	TTSAA	3 cut(s)	7, 538, 1419
AhdI	GACNNNNNGTC	1 cut(s)	679
AhlI	ACTAGT	1 cut(s)	908
AjiI	CACGTC	1 cut(s)	753
AjnI	CCWGG	3 cut(s)	364, 473, 1218
AjuI	GAANNNNNNNTTGG	4 cut(s)	777, 809, 1235, 1267
AleI	CACNNNNGTG	1 cut(s)	1754
Alw21I	GWGCWC	1 cut(s)	307
Alw26I	GTCTC	5 cut(s)	643, 666, 1472, 1486, 1572
AlwI	GGATC	3 cut(s)	882, 895, 1461
AlwNI	CAGNNNCTG	1 cut(s)	815
Ama87I	CYCGRG	3 cut(s)	300, 521, 669
Aor13HI	TCCGGA	1 cut(s)	107
AoxI	GGCC	3 cut(s)	762, 1138, 1384
ApeKI	GCWGC	6 cut(s)	485, 877, 1085, 1392, 1501, 1741
ApoI	RAATTY	3 cut(s)	30, 92, 1621
ArsI	GACNNNNNNTTYG	4 cut(s)	1176, 1208, 1277, 1309
AspLEI	GCGC	1 cut(s)	149
AspS9I	GGNCC	3 cut(s)	268, 757, 1139
AsuC2I	CCSGG	2 cut(s)	761, 1460
AsuHPI	GGTGA	5 cut(s)	152, 869, 1016, 1106, 1474
AvaI	CYCGRG	3 cut(s)	300, 521, 669
AvaII	GGWCC	2 cut(s)	268, 757
AxyI	CCTNAGG	1 cut(s)	1645
BamHI	GGATCC	1 cut(s)	887
BanI	GGYRCC	2 cut(s)	251, 1175
BanII	GRGCYC	2 cut(s)	307, 1362
BauI	CACGAG	2 cut(s)	956, 1110
BbsI	GAAGAC	1 cut(s)	727
Bbv12I	GWGCWC	1 cut(s)	307
BbvI	GCAGC	6 cut(s)	497, 864, 1072, 1404, 1513, 1728
BccI	CCATC	5 cut(s)	824, 1001, 1046, 1091, 1214
BceAI	ACGGC	2 cut(s)	582, 1292
BcgI	CGANNNNNNTGC	4 cut(s)	650, 684, 1507, 1541
BciT130I	CCWGG	3 cut(s)	366, 475, 1220
BclI	TGATCA	1 cut(s)	226
BcnI	CCSGG	2 cut(s)	761, 1460
BcoDI	GTCTC	5 cut(s)	643, 666, 1472, 1486, 1572
BcuI	ACTAGT	1 cut(s)	908
BfaI	CTAG	7 cut(s)	99, 245, 512, 602, 648, 782, 909
BfmI	CTRYAG	1 cut(s)	408
BglII	AGATCT	1 cut(s)	923
BisI	GCNGC	8 cut(s)	486, 878, 1086, 1393, 1440, 1443, 1502, 1742
BlpI	GCTNAGC	1 cut(s)	291
BlsI	GCNGC	8 cut(s)	487, 879, 1087, 1394, 1441, 1444, 1503, 1743
BmcAI	AGTACT	1 cut(s)	913
Bme1390I	CCNGG	5 cut(s)	366, 475, 761, 1220, 1460
Bme18I	GGWCC	2 cut(s)	268, 757
BmeRI	GACNNNNNGTC	1 cut(s)	679
BmeT110I	CYCGRG	3 cut(s)	300, 521, 669
BmgBI	CACGTC	1 cut(s)	753
BmgT120I	GGNCC	3 cut(s)	268, 757, 1139
BmiI	GGNNCC	6 cut(s)	253, 758, 759, 889, 1177, 1279
BmrFI	CCNGG	5 cut(s)	366, 475, 761, 1220, 1460
BmrI	ACTGGG	2 cut(s)	924, 1761
BmsI	GCATC	7 cut(s)	394, 740, 802, 1123, 1454, 1646, 1685
BmuI	ACTGGG	2 cut(s)	924, 1761
BoxI	GACNNNNGTC	1 cut(s)	755
BpiI	GAAGAC	1 cut(s)	727
BpmI	CTGGAG	1 cut(s)	1336
Bpu10I	CCTNAGC	1 cut(s)	825
Bpu1102I	GCTNAGC	1 cut(s)	291
BpuEI	CTTGAG	5 cut(s)	28, 410, 1346, 1365, 1677
BpuMI	CCSGG	2 cut(s)	761, 1460
BsaAI	YACGTR	1 cut(s)	937
BsaI	GGTCTC	2 cut(s)	1486, 1572
BsaJI	CCNNGG	8 cut(s)	364, 621, 642, 891, 1080, 1308, 1496, 1571
BsaWI	WCCGGW	1 cut(s)	107
BsaXI	ACNNNNNCTCC	2 cut(s)	1223, 1253
Bsc4I	CCNNNNNNNGG	8 cut(s)	371, 771, 1355, 1356, 1459, 1577, 1650, 1751
Bse118I	RCCGGY	1 cut(s)	1136
Bse1I	ACTGG	3 cut(s)	262, 919, 1756
Bse21I	CCTNAGG	1 cut(s)	1645
BseAI	TCCGGA	1 cut(s)	107
BseBI	CCWGG	3 cut(s)	366, 475, 1220
BseDI	CCNNGG	8 cut(s)	364, 621, 642, 891, 1080, 1308, 1496, 1571
BseGI	GGATG	5 cut(s)	104, 565, 835, 1329, 1546
BseLI	CCNNNNNNNGG	8 cut(s)	371, 771, 1355, 1356, 1459, 1577, 1650, 1751
BseMII	CTCAG	8 cut(s)	305, 569, 669, 780, 839, 1059, 1317, 1659
BseNI	ACTGG	3 cut(s)	262, 919, 1756
BseRI	GAGGAG	2 cut(s)	176, 187
BseXI	GCAGC	6 cut(s)	497, 864, 1072, 1404, 1513, 1728
BseYI	CCCAGC	1 cut(s)	944
BsgI	GTGCAG	2 cut(s)	504, 1572
Bsh1236I	CGCG	1 cut(s)	372
BshFI	GGCC	3 cut(s)	764, 1140, 1386
BshNI	GGYRCC	2 cut(s)	251, 1175
BsiHKAI	GWGCWC	1 cut(s)	307
BsiHKCI	CYCGRG	3 cut(s)	300, 521, 669
BsiSI	CCGG	4 cut(s)	108, 761, 1137, 1460
BslFI	GGGAC	3 cut(s)	743, 853, 961
BslI	CCNNNNNNNGG	8 cut(s)	371, 771, 1355, 1356, 1459, 1577, 1650, 1751
BsmAI	GTCTC	5 cut(s)	643, 666, 1472, 1486, 1572
BsmBI	CGTCTC	1 cut(s)	1472
BsmFI	GGGAC	3 cut(s)	743, 853, 961
BsmI	GAATGC	1 cut(s)	1703
BsnI	GGCC	3 cut(s)	764, 1140, 1386
Bso31I	GGTCTC	2 cut(s)	1486, 1572
BsoBI	CYCGRG	3 cut(s)	300, 521, 669
Bsp1286I	GDGCHC	2 cut(s)	307, 1362
Bsp13I	TCCGGA	1 cut(s)	107
Bsp143I	GATC	8 cut(s)	101, 226, 346, 887, 923, 1023, 1466, 1518
Bsp1720I	GCTNAGC	1 cut(s)	291
Bsp19I	CCATGG	1 cut(s)	621
BspACI	CCGC	7 cut(s)	23, 334, 372, 1346, 1356, 1439, 1442
BspANI	GGCC	3 cut(s)	764, 1140, 1386
BspCNI	CTCAG	8 cut(s)	304, 570, 670, 779, 838, 1058, 1316, 1658
BspEI	TCCGGA	1 cut(s)	107
BspFNI	CGCG	1 cut(s)	372
BspHI	TCATGA	2 cut(s)	349, 429
BspLI	GGNNCC	6 cut(s)	253, 758, 759, 889, 1177, 1279
BspPI	GGATC	3 cut(s)	882, 895, 1461
BspT107I	GGYRCC	2 cut(s)	251, 1175
BspTNI	GGTCTC	2 cut(s)	1486, 1572
BsrFI	RCCGGY	1 cut(s)	1136
BsrI	ACTGG	3 cut(s)	262, 919, 1756
BssAI	RCCGGY	1 cut(s)	1136
BssECI	CCNNGG	8 cut(s)	364, 621, 642, 891, 1080, 1308, 1496, 1571
BssMI	GATC	8 cut(s)	101, 226, 346, 887, 923, 1023, 1466, 1518
BssSI	CACGAG	2 cut(s)	956, 1110
BssT1I	CCWWGG	4 cut(s)	621, 642, 891, 1571
Bst2BI	CACGAG	2 cut(s)	956, 1110
Bst2UI	CCWGG	3 cut(s)	366, 475, 1220
Bst4CI	ACNGT	7 cut(s)	280, 1081, 1251, 1282, 1430, 1497, 1711
Bst6I	CTCTTC	1 cut(s)	1460
BstBAI	YACGTR	1 cut(s)	937
BstC8I	GCNNGC	3 cut(s)	297, 1138, 1358
BstDEI	CTNAG	9 cut(s)	291, 479, 578, 678, 766, 825, 1045, 1303, 1645
BstDSI	CCRYGG	4 cut(s)	621, 1080, 1308, 1496
BstENI	CCTNNNNNAGG	2 cut(s)	769, 1648
BstF5I	GGATG	5 cut(s)	104, 565, 835, 1329, 1546
BstFNI	CGCG	1 cut(s)	372
BstHHI	GCGC	1 cut(s)	149
BstKTI	GATC	8 cut(s)	104, 229, 349, 890, 926, 1026, 1469, 1521
BstMAI	GTCTC	5 cut(s)	643, 666, 1472, 1486, 1572
BstMBI	GATC	8 cut(s)	101, 226, 346, 887, 923, 1023, 1466, 1518
BstMWI	GCNNNNNNNGC	5 cut(s)	296, 704, 821, 1082, 1392
BstNI	CCWGG	3 cut(s)	366, 475, 1220
BstPAI	GACNNNNGTC	1 cut(s)	755
BstSCI	CCNGG	5 cut(s)	364, 473, 759, 1218, 1458
BstSFI	CTRYAG	1 cut(s)	408
BstSNI	TACGTA	1 cut(s)	937
BstUI	CGCG	1 cut(s)	372
BstV1I	GCAGC	6 cut(s)	497, 864, 1072, 1404, 1513, 1728
BstV2I	GAAGAC	1 cut(s)	727
BstX2I	RGATCY	3 cut(s)	887, 923, 1466
BstYI	RGATCY	3 cut(s)	887, 923, 1466
Bsu36I	CCTNAGG	1 cut(s)	1645
BsuRI	GGCC	3 cut(s)	764, 1140, 1386
BtgI	CCRYGG	4 cut(s)	621, 1080, 1308, 1496
BtgZI	GCGATG	1 cut(s)	1090
BtrI	CACGTC	1 cut(s)	753
BtsCI	GGATG	5 cut(s)	104, 565, 835, 1329, 1546
BtsI	GCAGTG	2 cut(s)	640, 870
BtsIMutI	CAGTG	4 cut(s)	640, 675, 870, 1749
Cac8I	GCNNGC	3 cut(s)	297, 1138, 1358
CaiI	CAGNNNCTG	1 cut(s)	815
CciI	TCATGA	2 cut(s)	349, 429
CfoI	GCGC	1 cut(s)	149
Cfr10I	RCCGGY	1 cut(s)	1136
Cfr13I	GGNCC	3 cut(s)	268, 757, 1139
CseI	GACGC	1 cut(s)	850
Csp6I	GTAC	5 cut(s)	906, 912, 961, 1603, 1615
CviAII	CATG	7 cut(s)	350, 430, 622, 1525, 1537, 1618, 1673
CviQI	GTAC	5 cut(s)	906, 912, 961, 1603, 1615
DdeI	CTNAG	9 cut(s)	291, 479, 578, 678, 766, 825, 1045, 1303, 1645
DpnI	GATC	8 cut(s)	103, 228, 348, 889, 925, 1025, 1468, 1520
DpnII	GATC	8 cut(s)	101, 226, 346, 887, 923, 1023, 1466, 1518
DrdI	GACNNNNNNGTC	2 cut(s)	442, 1187
DriI	GACNNNNNGTC	1 cut(s)	679
DseDI	GACNNNNNNGTC	2 cut(s)	442, 1187
Eam1104I	CTCTTC	1 cut(s)	1460
Eam1105I	GACNNNNNGTC	1 cut(s)	679
EarI	CTCTTC	1 cut(s)	1460
Ecl136II	GAGCTC	1 cut(s)	305
Eco105I	TACGTA	1 cut(s)	937
Eco130I	CCWWGG	4 cut(s)	621, 642, 891, 1571
Eco24I	GRGCYC	2 cut(s)	307, 1362
Eco31I	GGTCTC	2 cut(s)	1486, 1572
Eco32I	GATATC	1 cut(s)	1670
Eco47I	GGWCC	2 cut(s)	268, 757
Eco53kI	GAGCTC	1 cut(s)	305
Eco81I	CCTNAGG	1 cut(s)	1645
Eco88I	CYCGRG	3 cut(s)	300, 521, 669
EcoICRI	GAGCTC	1 cut(s)	305
EcoNI	CCTNNNNNAGG	2 cut(s)	769, 1648
EcoO109I	RGGNCCY	1 cut(s)	757
EcoRI	GAATTC	1 cut(s)	1621
EcoRII	CCWGG	3 cut(s)	364, 473, 1218
EcoRV	GATATC	1 cut(s)	1670
EcoT14I	CCWWGG	4 cut(s)	621, 642, 891, 1571
EcoT22I	ATGCAT	1 cut(s)	1678
EcoT38I	GRGCYC	2 cut(s)	307, 1362
ErhI	CCWWGG	4 cut(s)	621, 642, 891, 1571
Esp3I	CGTCTC	1 cut(s)	1472
FaeI	CATG	7 cut(s)	353, 433, 625, 1528, 1540, 1621, 1676
FaqI	GGGAC	3 cut(s)	743, 853, 961
FatI	CATG	7 cut(s)	349, 429, 621, 1524, 1536, 1617, 1672
FauI	CCCGC	1 cut(s)	1349
FbaI	TGATCA	1 cut(s)	226
FblI	GTMKAC	1 cut(s)	1284
Fnu4HI	GCNGC	8 cut(s)	486, 878, 1086, 1393, 1440, 1443, 1502, 1742
FokI	GGATG	5 cut(s)	91, 572, 842, 1316, 1553
FriOI	GRGCYC	2 cut(s)	307, 1362
Fsp4HI	GCNGC	8 cut(s)	486, 878, 1086, 1393, 1440, 1443, 1502, 1742
FspBI	CTAG	7 cut(s)	99, 245, 512, 602, 648, 782, 909
FspI	TGCGCA	1 cut(s)	148
GlaI	GCGC	1 cut(s)	148
GluI	GCNGC	8 cut(s)	486, 878, 1086, 1393, 1440, 1443, 1502, 1742
GsaI	CCCAGC	1 cut(s)	948
GsuI	CTGGAG	1 cut(s)	1336
HaeIII	GGCC	3 cut(s)	764, 1140, 1386
HapII	CCGG	4 cut(s)	108, 761, 1137, 1460
HgaI	GACGC	1 cut(s)	850
HhaI	GCGC	1 cut(s)	149
Hin1II	CATG	7 cut(s)	353, 433, 625, 1528, 1540, 1621, 1676
Hin6I	GCGC	1 cut(s)	147
HinP1I	GCGC	1 cut(s)	147
HincII	GTYRAC	1 cut(s)	1285
HindII	GTYRAC	1 cut(s)	1285
HindIII	AAGCTT	3 cut(s)	63, 776, 786
HinfI	GANTC	5 cut(s)	176, 680, 1170, 1544, 1732
HpaII	CCGG	4 cut(s)	108, 761, 1137, 1460
HphI	GGTGA	5 cut(s)	152, 869, 1016, 1106, 1474
Hpy166II	GTNNAC	7 cut(s)	358, 379, 469, 1129, 1195, 1285, 1689
Hpy188I	TCNGA	9 cut(s)	226, 579, 658, 736, 750, 769, 1048, 1404, 1450
Hpy8I	GTNNAC	7 cut(s)	358, 379, 469, 1129, 1195, 1285, 1689
Hpy99I	CGWCG	3 cut(s)	467, 754, 1286
HpyAV	CCTTC	6 cut(s)	81, 242, 862, 1152, 1644, 1755
HpyCH4III	ACNGT	7 cut(s)	280, 1081, 1251, 1282, 1430, 1497, 1711
HpyCH4IV	ACGT	6 cut(s)	465, 752, 936, 990, 1481, 1685
HpyCH4V	TGCA	6 cut(s)	485, 707, 1326, 1528, 1589, 1676
HpyF10VI	GCNNNNNNNGC	5 cut(s)	296, 704, 821, 1082, 1392
HpyF3I	CTNAG	9 cut(s)	291, 479, 578, 678, 766, 825, 1045, 1303, 1645
HpySE526I	ACGT	6 cut(s)	465, 752, 936, 990, 1481, 1685
Hsp92II	CATG	7 cut(s)	353, 433, 625, 1528, 1540, 1621, 1676
HspAI	GCGC	1 cut(s)	147
KflI	GGGWCCC	1 cut(s)	757
Kpn2I	TCCGGA	1 cut(s)	107
KroI	GCCGGC	1 cut(s)	1136
KroNI	GCCGGC	1 cut(s)	1138
Ksp22I	TGATCA	1 cut(s)	226
Kzo9I	GATC	8 cut(s)	101, 226, 346, 887, 923, 1023, 1466, 1518
LmnI	GCTCC	1 cut(s)	658
Lsp1109I	GCAGC	6 cut(s)	497, 864, 1072, 1404, 1513, 1728
LweI	GCATC	7 cut(s)	394, 740, 802, 1123, 1454, 1646, 1685
MaeI	CTAG	7 cut(s)	99, 245, 512, 602, 648, 782, 909
MaeII	ACGT	6 cut(s)	465, 752, 936, 990, 1481, 1685
MaeIII	GTNAC	2 cut(s)	562, 1372
MalI	GATC	8 cut(s)	103, 228, 348, 889, 925, 1025, 1468, 1520
MboI	GATC	8 cut(s)	101, 226, 346, 887, 923, 1023, 1466, 1518
MboII	GAAGA	4 cut(s)	31, 732, 1285, 1447
MflI	RGATCY	3 cut(s)	887, 923, 1466
MhlI	GDGCHC	2 cut(s)	307, 1362
MluCI	AATT	6 cut(s)	9, 30, 92, 882, 1621, 1776
MlyI	GAGTC	3 cut(s)	689, 1179, 1538
MmeI	TCCRAC	1 cut(s)	773
Mph1103I	ATGCAT	1 cut(s)	1678
MroI	TCCGGA	1 cut(s)	107
MroNI	GCCGGC	1 cut(s)	1136
MseI	TTAA	2 cut(s)	1593, 1656
MslI	CAYNNNNRTG	1 cut(s)	1754
MspA1I	CMGCKG	1 cut(s)	1356
MspI	CCGG	4 cut(s)	108, 761, 1137, 1460
MspR9I	CCNGG	5 cut(s)	366, 475, 761, 1220, 1460
Mva1269I	GAATGC	1 cut(s)	1703
MvaI	CCWGG	3 cut(s)	366, 475, 1220
MvnI	CGCG	1 cut(s)	372
MwoI	GCNNNNNNNGC	5 cut(s)	296, 704, 821, 1082, 1392
NaeI	GCCGGC	1 cut(s)	1138
NciI	CCSGG	2 cut(s)	761, 1460
NcoI	CCATGG	1 cut(s)	621
NdeII	GATC	8 cut(s)	101, 226, 346, 887, 923, 1023, 1466, 1518
NgoMIV	GCCGGC	1 cut(s)	1136
NlaIII	CATG	7 cut(s)	353, 433, 625, 1528, 1540, 1621, 1676
NlaIV	GGNNCC	6 cut(s)	253, 758, 759, 889, 1177, 1279
NmuCI	GTSAC	2 cut(s)	562, 1372
NsbI	TGCGCA	1 cut(s)	148
NsiI	ATGCAT	1 cut(s)	1678
OliI	CACNNNNGTG	1 cut(s)	1754
PaeR7I	CTCGAG	3 cut(s)	300, 521, 669
PagI	TCATGA	2 cut(s)	349, 429
PcsI	WCGNNNNNNNCGW	1 cut(s)	462
PctI	GAATGC	1 cut(s)	1703
PdiI	GCCGGC	1 cut(s)	1138
PfeI	GAWTC	2 cut(s)	176, 1732
PflFI	GACNNNGTC	1 cut(s)	425
PkrI	GCNGC	8 cut(s)	487, 879, 1087, 1394, 1441, 1444, 1503, 1743
PleI	GAGTC	3 cut(s)	688, 1178, 1538
PpsI	GAGTC	3 cut(s)	688, 1178, 1538
Ppu21I	YACGTR	1 cut(s)	937
PpuMI	RGGWCCY	1 cut(s)	757
PshAI	GACNNNNGTC	1 cut(s)	755
PsiI	TTATAA	1 cut(s)	1068
Psp124BI	GAGCTC	1 cut(s)	307
Psp5II	RGGWCCY	1 cut(s)	757
Psp6I	CCWGG	3 cut(s)	364, 473, 1218
PspFI	CCCAGC	1 cut(s)	944
PspGI	CCWGG	3 cut(s)	364, 473, 1218
PspN4I	GGNNCC	6 cut(s)	253, 758, 759, 889, 1177, 1279
PspPI	GGNCC	3 cut(s)	268, 757, 1139
PspPPI	RGGWCCY	1 cut(s)	757
PspXI	VCTCGAGB	1 cut(s)	300
PstNI	CAGNNNCTG	1 cut(s)	815
PsuI	RGATCY	3 cut(s)	887, 923, 1466
PsyI	GACNNNGTC	1 cut(s)	425
RsaI	GTAC	5 cut(s)	907, 913, 962, 1604, 1616
RsaNI	GTAC	5 cut(s)	906, 912, 961, 1603, 1615
RseI	CAYNNNNRTG	1 cut(s)	1754
SacI	GAGCTC	1 cut(s)	307
SalI	GTCGAC	1 cut(s)	1283
SaqAI	TTAA	2 cut(s)	1593, 1656
SatI	GCNGC	8 cut(s)	486, 878, 1086, 1393, 1440, 1443, 1502, 1742
Sau3AI	GATC	8 cut(s)	101, 226, 346, 887, 923, 1023, 1466, 1518
Sau96I	GGNCC	3 cut(s)	268, 757, 1139
ScaI	AGTACT	1 cut(s)	913
SchI	GAGTC	3 cut(s)	689, 1179, 1538
ScrFI	CCNGG	5 cut(s)	366, 475, 761, 1220, 1460
SduI	GDGCHC	2 cut(s)	307, 1362
SfaNI	GCATC	7 cut(s)	394, 740, 802, 1123, 1454, 1646, 1685
SfcI	CTRYAG	1 cut(s)	408
Sfr274I	CTCGAG	3 cut(s)	300, 521, 669
SinI	GGWCC	2 cut(s)	268, 757
SlaI	CTCGAG	3 cut(s)	300, 521, 669
SmiMI	CAYNNNNRTG	1 cut(s)	1754
SmlI	CTYRAG	8 cut(s)	43, 300, 389, 521, 669, 1361, 1380, 1692
SmoI	CTYRAG	8 cut(s)	43, 300, 389, 521, 669, 1361, 1380, 1692
SnaBI	TACGTA	1 cut(s)	937
SpeI	ACTAGT	1 cut(s)	908
Sse9I	AATT	6 cut(s)	9, 30, 92, 882, 1621, 1776
SsiI	CCGC	7 cut(s)	23, 334, 372, 1346, 1356, 1439, 1442
SspMI	CTAG	7 cut(s)	99, 245, 512, 602, 648, 782, 909
SstI	GAGCTC	1 cut(s)	307
StyD4I	CCNGG	5 cut(s)	364, 473, 759, 1218, 1458
StyI	CCWWGG	4 cut(s)	621, 642, 891, 1571
TaaI	ACNGT	7 cut(s)	280, 1081, 1251, 1282, 1430, 1497, 1711
TaiI	ACGT	6 cut(s)	468, 755, 939, 993, 1484, 1688
TaqII	GACCGA	1 cut(s)	256
TasI	AATT	6 cut(s)	9, 30, 92, 882, 1621, 1776
TatI	WGTACW	4 cut(s)	911, 960, 1602, 1614
TauI	GCSGC	2 cut(s)	1442, 1445
TfiI	GAWTC	2 cut(s)	176, 1732
Tru1I	TTAA	2 cut(s)	1593, 1656
Tru9I	TTAA	2 cut(s)	1593, 1656
TscAI	CASTG	4 cut(s)	640, 682, 870, 1756
TseFI	GTSAC	2 cut(s)	562, 1372
TseI	GCWGC	6 cut(s)	485, 877, 1085, 1392, 1501, 1741
Tsp45I	GTSAC	2 cut(s)	562, 1372
TspDTI	ATGAA	5 cut(s)	1093, 1279, 1525, 1634, 1711
TspRI	CASTG	4 cut(s)	640, 682, 870, 1756
Tth111I	GACNNNGTC	1 cut(s)	425
VpaK11BI	GGWCC	2 cut(s)	268, 757
XagI	CCTNNNNNAGG	2 cut(s)	769, 1648
XapI	RAATTY	3 cut(s)	30, 92, 1621
XbaI	TCTAGA	3 cut(s)	98, 511, 781
XhoI	CTCGAG	3 cut(s)	300, 521, 669
XmiI	GTMKAC	1 cut(s)	1284
XspI	CTAG	7 cut(s)	99, 245, 512, 602, 648, 782, 909
ZrmI	AGTACT	1 cut(s)	913
Zsp2I	ATGCAT	1 cut(s)	1678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.