RLG00000008522

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011630
Physical Location & Seq
Forward (+)
31643968 .. 31646640
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008522

Sequence Viewer

Length: 1812 bp
ATGCCTGTCTCTCTCTCTGCCATAAGCATGTTCAAAATTATGTTCTTGGCGGTGGAAATTTTATTCTTCTCAAGCATATTATTAGCAAAAGCTTCTCCTTCACAATCCCATTTCTCCAAATTTTCTAGATCATCCGGAGGACTTTCCCATAAAGAAGCCATTTTATCTCTGTGCGCACATACCCCTCACCAAGCAACTTGTGAATCCACTCTCCTCTCCTCCTCCTCCTCATCATCATCTACAAGAGGCTCTGATCATCTCCCTCATACCCTAGAAGGTGCCTTTGTCCAGTCGGTCCATTCCACTGTTGCGAAAGCTCAGCACGCTCAAGCTCTCGCCTATAATCTCACTATTTCCCACCGCCAAAATGCCGATCATGATGTTCACTTCCCTGGTCGCGGTGTGAACGACTGCCTTGAGCTTCTGGATGATGCTATAGATATGCTCGACAATATCATGATGACATCGTTGTCGAAACCTAACGATGACGACGTTCACACCTGGCTCAGTGCAGCTCTTACGAACCAAGAAACTTGTCTAGAGAGCCTCGAGAAAGACAAGTTCAAGACAGAGAAAAACTTTATGGATGTCACGGCTCAAAATCTGAGCCAATACATAAGCAACTCCCTAGCACTTTTCGTGCTTACCTTGGGTGGTAGCAGTGTCTCCTTCGCTAGTGGTTCGGAGCATTTACCTCGAGACACTGAGTCAAAGTCAAAGGAAGCTAAAAATGCAAACCCTAATAGAAGACTATTGGCTTCGGACAGTAGTTTTCCGACGTGGGTCCCAGCAGCAGAGAGGAAGCTTCTAGAAGCTTCTGTTGGGGAGATAGAAGCAGATGCTGTGGTGGCTCAGGATGGGAGTGGGACGCACAAGAGCATTGGTGAGGCAGTGAAGGGGTTGCTGCTCATTGGATCCTTGGAGAGTAGTGGTACTAGTACTGGGGGGAGATCTGTGATTCAAGTAAAAGCTGGGACATACCACGAGTACATAAACATACCCACGAAGCAAAAGAACGTGATGTTGATTGGTGATGGAGTGGGGAAAACGATCATTGTGGGCAACAGGAACTCAGATGATGGCTGGACAACATATAAGTCTGCTACTGTGGCTGCTATGGGTGATGGGTTCATCGCTCGTGATATAACATTTGTGAACGACGCCGGACCTTCCAAGCACCAAGCAGTCGCACTTCGAGTCGGTGCCGATAAATCGGTCGTCTACCGATGTTCTATCATAGGCTACCAGGACACTCTCTACACTCACTCCAAGCGACAGTTCTACCGAGAAACCGACATCTATGGAACCGTCGACTTCATCTTCGGCAACTCAGCCGTGGTCTTCCAAACTTGCAACATCTACCCCCGAAAACCGCCCTCCAGCGGGCTCAAGAACTTTGTCACAGCTCAAGGCCGAAGCAGCCCTGATCAGAACACAGGCATTTCAATCCACAACTGCAAGATAGCGGCGGCATCAGACCTCTTCCCGGTGAGATCCAGCTACGAGACGTATCTTGGGAGACCGTGGAAGCAGTATTCGAGAACGATCATCATGCAGTCTTTCATGGATGACTCGATAAATCGAGCAGGGTGGTCTCCTTGGTCTGGTGGGTCGGCACCTAACACATTGTACTATGGAGAGTACATGAATTCTGGGTCTGGGGCATCAACCTTAGGAAGGGTTAAATGGGGTGGATATCATGCATCACTTACGTCTACTCAAGCACAGGCATTCACAGTTGGGGGTTTCATATCTGGGAATCTTTGGCTGCCTTCCACTGGGGTGTCTTTTAGTTCTGGACTAATTGGATAA

Protein Analysis

604

Amino Acids

64.94

Weight (kDa)

6.83

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 53 - 212 9.5e-26 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 280 - 587 4.5e-134 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 469
Acc16I TGCGCA 1 cut(s) 175
AccB1I GGYRCC 3 cut(s) 278, 1202, 1615
AccI GTMKAC 3 cut(s) 1221, 1311, 1715
AccII CGCG 1 cut(s) 399
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 7 cut(s) 50, 361, 399, 1373, 1383, 1466, 1469
AclWI GGATC 3 cut(s) 909, 922, 1488
AcsI RAATTY 3 cut(s) 57, 119, 1648
AcyI GRCGYC 1 cut(s) 1161
AfaI GTAC 5 cut(s) 934, 940, 989, 1631, 1643
AfiI CCNNNNNNNGG 7 cut(s) 398, 1382, 1383, 1486, 1604, 1677, 1778
AgsI TTSAA 4 cut(s) 34, 565, 962, 1446
AhdI GACNNNNNGTC 1 cut(s) 706
AhlI ACTAGT 1 cut(s) 935
AjiI CACGTC 1 cut(s) 780
AjnI CCWGG 3 cut(s) 391, 500, 1245
AjuI GAANNNNNNNTTGG 4 cut(s) 804, 836, 1262, 1294
AleI CACNNNNGTG 1 cut(s) 1781
Alw26I GTCTC 6 cut(s) 13, 670, 693, 1499, 1513, 1599
AlwI GGATC 3 cut(s) 909, 922, 1488
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 2 cut(s) 548, 696
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 1 cut(s) 1411
ApeKI GCWGC 6 cut(s) 512, 791, 904, 1112, 1419, 1768
ApoI RAATTY 3 cut(s) 57, 119, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 1304, 1336
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 295, 784, 1166
AsuC2I CCSGG 1 cut(s) 1487
AsuHPI GGTGA 5 cut(s) 179, 896, 1043, 1133, 1501
AvaI CYCGRG 2 cut(s) 548, 696
AvaII GGWCC 3 cut(s) 295, 784, 1166
AxyI CCTNAGG 1 cut(s) 1672
BamHI GGATCC 1 cut(s) 914
BanI GGYRCC 3 cut(s) 278, 1202, 1615
BanII GRGCYC 1 cut(s) 1389
BauI CACGAG 2 cut(s) 983, 1137
BbsI GAAGAC 2 cut(s) 754, 1333
BbvI GCAGC 6 cut(s) 524, 803, 891, 1099, 1431, 1755
BccI CCATC 4 cut(s) 851, 1028, 1073, 1118
BceAI ACGGC 2 cut(s) 609, 1319
BcgI CGANNNNNNTGC 4 cut(s) 677, 711, 1534, 1568
BciT130I CCWGG 3 cut(s) 393, 502, 1247
BclI TGATCA 2 cut(s) 253, 1426
BcnI CCSGG 1 cut(s) 1487
BcoDI GTCTC 6 cut(s) 13, 670, 693, 1499, 1513, 1599
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 7 cut(s) 126, 272, 539, 629, 675, 809, 936
BfmI CTRYAG 1 cut(s) 435
BglII AGATCT 1 cut(s) 950
BisI GCNGC 8 cut(s) 513, 792, 905, 1113, 1420, 1467, 1470, 1769
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 8 cut(s) 514, 793, 906, 1114, 1421, 1468, 1471, 1770
BmcAI AGTACT 1 cut(s) 940
Bme1390I CCNGG 4 cut(s) 393, 502, 1247, 1487
Bme18I GGWCC 3 cut(s) 295, 784, 1166
BmeRI GACNNNNNGTC 1 cut(s) 706
BmeT110I CYCGRG 2 cut(s) 548, 696
BmgBI CACGTC 1 cut(s) 780
BmgT120I GGNCC 3 cut(s) 295, 784, 1166
BmiI GGNNCC 7 cut(s) 280, 785, 786, 916, 1204, 1306, 1617
BmrFI CCNGG 4 cut(s) 393, 502, 1247, 1487
BmrI ACTGGG 2 cut(s) 951, 1788
BmsI GCATC 5 cut(s) 421, 829, 1481, 1673, 1712
BmuI ACTGGG 2 cut(s) 951, 1788
BoxI GACNNNNGTC 1 cut(s) 782
BpiI GAAGAC 2 cut(s) 754, 1333
BpmI CTGGAG 1 cut(s) 1363
Bpu10I CCTNAGC 1 cut(s) 852
Bpu1102I GCTNAGC 1 cut(s) 318
BpuEI CTTGAG 6 cut(s) 55, 312, 437, 1373, 1392, 1704
BpuMI CCSGG 1 cut(s) 1487
BsaHI GRCGYC 1 cut(s) 1161
BsaI GGTCTC 2 cut(s) 1513, 1599
BsaJI CCNNGG 6 cut(s) 391, 648, 918, 1335, 1523, 1598
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 1250, 1280
Bsc4I CCNNNNNNNGG 7 cut(s) 398, 1382, 1383, 1486, 1604, 1677, 1778
Bse1I ACTGG 3 cut(s) 289, 946, 1783
Bse21I CCTNAGG 1 cut(s) 1672
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 3 cut(s) 393, 502, 1247
BseDI CCNNGG 6 cut(s) 391, 648, 918, 1335, 1523, 1598
BseGI GGATG 5 cut(s) 131, 433, 592, 862, 1573
BseLI CCNNNNNNNGG 7 cut(s) 398, 1382, 1383, 1486, 1604, 1677, 1778
BseMII CTCAG 7 cut(s) 332, 520, 596, 696, 866, 1086, 1344
BseNI ACTGG 3 cut(s) 289, 946, 1783
BseRI GAGGAG 5 cut(s) 203, 208, 211, 214, 217
BseXI GCAGC 6 cut(s) 524, 803, 891, 1099, 1431, 1755
BseYI CCCAGC 2 cut(s) 787, 971
BsgI GTGCAG 1 cut(s) 531
Bsh1236I CGCG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 1218
BshFI GGCC 1 cut(s) 1413
BshNI GGYRCC 3 cut(s) 278, 1202, 1615
BsiEI CGRYCG 1 cut(s) 1218
BsiHKCI CYCGRG 2 cut(s) 548, 696
BsiSI CCGG 3 cut(s) 135, 1164, 1487
BslFI GGGAC 3 cut(s) 770, 880, 988
BslI CCNNNNNNNGG 7 cut(s) 398, 1382, 1383, 1486, 1604, 1677, 1778
BsmAI GTCTC 6 cut(s) 13, 670, 693, 1499, 1513, 1599
BsmBI CGTCTC 1 cut(s) 1499
BsmFI GGGAC 3 cut(s) 770, 880, 988
BsmI GAATGC 1 cut(s) 1730
BsnI GGCC 1 cut(s) 1413
Bso31I GGTCTC 2 cut(s) 1513, 1599
BsoBI CYCGRG 2 cut(s) 548, 696
Bsp1286I GDGCHC 1 cut(s) 1389
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 9 cut(s) 128, 253, 373, 914, 950, 1050, 1426, 1493, 1545
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 7 cut(s) 50, 361, 399, 1373, 1383, 1466, 1469
BspANI GGCC 1 cut(s) 1413
BspCNI CTCAG 7 cut(s) 331, 519, 597, 697, 865, 1085, 1343
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 1 cut(s) 399
BspHI TCATGA 2 cut(s) 376, 456
BspLI GGNNCC 7 cut(s) 280, 785, 786, 916, 1204, 1306, 1617
BspPI GGATC 3 cut(s) 909, 922, 1488
BspT107I GGYRCC 3 cut(s) 278, 1202, 1615
BspTNI GGTCTC 2 cut(s) 1513, 1599
BsrI ACTGG 3 cut(s) 289, 946, 1783
BssECI CCNNGG 6 cut(s) 391, 648, 918, 1335, 1523, 1598
BssMI GATC 9 cut(s) 128, 253, 373, 914, 950, 1050, 1426, 1493, 1545
BssNI GRCGYC 1 cut(s) 1161
BssSI CACGAG 2 cut(s) 983, 1137
BssT1I CCWWGG 3 cut(s) 648, 918, 1598
Bst2BI CACGAG 2 cut(s) 983, 1137
Bst2UI CCWGG 3 cut(s) 393, 502, 1247
Bst4CI ACNGT 7 cut(s) 307, 767, 1108, 1278, 1309, 1524, 1738
Bst6I CTCTTC 1 cut(s) 1487
BstACI GRCGYC 1 cut(s) 1161
BstC8I GCNNGC 2 cut(s) 324, 1385
BstDEI CTNAG 8 cut(s) 318, 506, 605, 705, 852, 1072, 1330, 1672
BstDSI CCRYGG 2 cut(s) 1335, 1523
BstENI CCTNNNNNAGG 1 cut(s) 1675
BstF5I GGATG 5 cut(s) 131, 433, 592, 862, 1573
BstFNI CGCG 1 cut(s) 399
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 9 cut(s) 131, 256, 376, 917, 953, 1053, 1429, 1496, 1548
BstMAI GTCTC 6 cut(s) 13, 670, 693, 1499, 1513, 1599
BstMBI GATC 9 cut(s) 128, 253, 373, 914, 950, 1050, 1426, 1493, 1545
BstMCI CGRYCG 1 cut(s) 1218
BstMWI GCNNNNNNNGC 5 cut(s) 323, 731, 848, 1109, 1419
BstNI CCWGG 3 cut(s) 393, 502, 1247
BstNSI RCATGY 1 cut(s) 31
BstPAI GACNNNNGTC 1 cut(s) 782
BstSCI CCNGG 4 cut(s) 391, 500, 1245, 1485
BstSFI CTRYAG 1 cut(s) 435
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 6 cut(s) 524, 803, 891, 1099, 1431, 1755
BstV2I GAAGAC 2 cut(s) 754, 1333
BstX2I RGATCY 3 cut(s) 914, 950, 1493
BstYI RGATCY 3 cut(s) 914, 950, 1493
Bsu36I CCTNAGG 1 cut(s) 1672
BsuRI GGCC 1 cut(s) 1413
BtgI CCRYGG 2 cut(s) 1335, 1523
BtgZI GCGATG 1 cut(s) 1117
BtrI CACGTC 1 cut(s) 780
BtsCI GGATG 5 cut(s) 131, 433, 592, 862, 1573
BtsI GCAGTG 2 cut(s) 667, 897
BtsIMutI CAGTG 6 cut(s) 303, 514, 667, 702, 897, 1776
Cac8I GCNNGC 2 cut(s) 324, 1385
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 2 cut(s) 376, 456
CfoI GCGC 1 cut(s) 176
Cfr13I GGNCC 3 cut(s) 295, 784, 1166
CseI GACGC 2 cut(s) 877, 1169
Csp6I GTAC 5 cut(s) 933, 939, 988, 1630, 1642
CviAII CATG 7 cut(s) 28, 377, 457, 1552, 1564, 1645, 1700
CviQI GTAC 5 cut(s) 933, 939, 988, 1630, 1642
DdeI CTNAG 8 cut(s) 318, 506, 605, 705, 852, 1072, 1330, 1672
DpnI GATC 9 cut(s) 130, 255, 375, 916, 952, 1052, 1428, 1495, 1547
DpnII GATC 9 cut(s) 128, 253, 373, 914, 950, 1050, 1426, 1493, 1545
DrdI GACNNNNNNGTC 1 cut(s) 469
DriI GACNNNNNGTC 1 cut(s) 706
DseDI GACNNNNNNGTC 1 cut(s) 469
Eam1104I CTCTTC 1 cut(s) 1487
Eam1105I GACNNNNNGTC 1 cut(s) 706
EarI CTCTTC 1 cut(s) 1487
Eco130I CCWWGG 3 cut(s) 648, 918, 1598
Eco24I GRGCYC 1 cut(s) 1389
Eco31I GGTCTC 2 cut(s) 1513, 1599
Eco32I GATATC 1 cut(s) 1697
Eco47I GGWCC 3 cut(s) 295, 784, 1166
Eco81I CCTNAGG 1 cut(s) 1672
Eco88I CYCGRG 2 cut(s) 548, 696
EcoNI CCTNNNNNAGG 1 cut(s) 1675
EcoO109I RGGNCCY 1 cut(s) 784
EcoRI GAATTC 1 cut(s) 1648
EcoRII CCWGG 3 cut(s) 391, 500, 1245
EcoRV GATATC 1 cut(s) 1697
EcoT14I CCWWGG 3 cut(s) 648, 918, 1598
EcoT22I ATGCAT 1 cut(s) 1705
EcoT38I GRGCYC 1 cut(s) 1389
ErhI CCWWGG 3 cut(s) 648, 918, 1598
Esp3I CGTCTC 1 cut(s) 1499
FaeI CATG 7 cut(s) 31, 380, 460, 1555, 1567, 1648, 1703
FaqI GGGAC 3 cut(s) 770, 880, 988
FatI CATG 7 cut(s) 27, 376, 456, 1551, 1563, 1644, 1699
FauI CCCGC 1 cut(s) 1376
FbaI TGATCA 2 cut(s) 253, 1426
FblI GTMKAC 3 cut(s) 1221, 1311, 1715
Fnu4HI GCNGC 8 cut(s) 513, 792, 905, 1113, 1420, 1467, 1470, 1769
FokI GGATG 5 cut(s) 118, 440, 599, 869, 1580
FriOI GRGCYC 1 cut(s) 1389
Fsp4HI GCNGC 8 cut(s) 513, 792, 905, 1113, 1420, 1467, 1470, 1769
FspAI RTGCGCAY 1 cut(s) 175
FspBI CTAG 7 cut(s) 126, 272, 539, 629, 675, 809, 936
FspI TGCGCA 1 cut(s) 175
GlaI GCGC 1 cut(s) 175
GluI GCNGC 8 cut(s) 513, 792, 905, 1113, 1420, 1467, 1470, 1769
GsaI CCCAGC 2 cut(s) 791, 975
GsuI CTGGAG 1 cut(s) 1363
HaeIII GGCC 1 cut(s) 1413
HapII CCGG 3 cut(s) 135, 1164, 1487
HgaI GACGC 2 cut(s) 877, 1169
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 1 cut(s) 1161
Hin1II CATG 7 cut(s) 31, 380, 460, 1555, 1567, 1648, 1703
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 1312
HindII GTYRAC 1 cut(s) 1312
HindIII AAGCTT 3 cut(s) 90, 803, 813
HinfI GANTC 6 cut(s) 203, 707, 958, 1197, 1571, 1759
HpaII CCGG 3 cut(s) 135, 1164, 1487
HphI GGTGA 5 cut(s) 179, 896, 1043, 1133, 1501
Hpy166II GTNNAC 7 cut(s) 385, 406, 496, 1156, 1222, 1312, 1716
Hpy188I TCNGA 8 cut(s) 253, 606, 685, 763, 777, 1075, 1431, 1477
Hpy8I GTNNAC 7 cut(s) 385, 406, 496, 1156, 1222, 1312, 1716
Hpy99I CGWCG 4 cut(s) 494, 781, 1163, 1313
HpyAV CCTTC 7 cut(s) 108, 269, 679, 889, 1179, 1671, 1782
HpyCH4III ACNGT 7 cut(s) 307, 767, 1108, 1278, 1309, 1524, 1738
HpyCH4IV ACGT 5 cut(s) 492, 779, 1017, 1508, 1712
HpyCH4V TGCA 6 cut(s) 512, 734, 1353, 1458, 1555, 1703
HpyF10VI GCNNNNNNNGC 5 cut(s) 323, 731, 848, 1109, 1419
HpyF3I CTNAG 8 cut(s) 318, 506, 605, 705, 852, 1072, 1330, 1672
HpySE526I ACGT 5 cut(s) 492, 779, 1017, 1508, 1712
Hsp92I GRCGYC 1 cut(s) 1161
Hsp92II CATG 7 cut(s) 31, 380, 460, 1555, 1567, 1648, 1703
HspAI GCGC 1 cut(s) 174
KflI GGGWCCC 1 cut(s) 784
Kpn2I TCCGGA 1 cut(s) 134
Ksp22I TGATCA 2 cut(s) 253, 1426
Kzo9I GATC 9 cut(s) 128, 253, 373, 914, 950, 1050, 1426, 1493, 1545
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 6 cut(s) 524, 803, 891, 1099, 1431, 1755
LweI GCATC 5 cut(s) 421, 829, 1481, 1673, 1712
MaeI CTAG 7 cut(s) 126, 272, 539, 629, 675, 809, 936
MaeII ACGT 5 cut(s) 492, 779, 1017, 1508, 1712
MaeIII GTNAC 2 cut(s) 589, 1399
MalI GATC 9 cut(s) 130, 255, 375, 916, 952, 1052, 1428, 1495, 1547
MboI GATC 9 cut(s) 128, 253, 373, 914, 950, 1050, 1426, 1493, 1545
MboII GAAGA 5 cut(s) 58, 759, 1312, 1333, 1474
MflI RGATCY 3 cut(s) 914, 950, 1493
MhlI GDGCHC 1 cut(s) 1389
MluCI AATT 5 cut(s) 36, 57, 119, 1648, 1803
MlyI GAGTC 3 cut(s) 716, 1206, 1565
MmeI TCCRAC 1 cut(s) 800
Mph1103I ATGCAT 1 cut(s) 1705
MroI TCCGGA 1 cut(s) 134
MseI TTAA 1 cut(s) 1683
MslI CAYNNNNRTG 2 cut(s) 26, 1781
MspA1I CMGCKG 1 cut(s) 1383
MspI CCGG 3 cut(s) 135, 1164, 1487
MspR9I CCNGG 4 cut(s) 393, 502, 1247, 1487
Mva1269I GAATGC 1 cut(s) 1730
MvaI CCWGG 3 cut(s) 393, 502, 1247
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 5 cut(s) 323, 731, 848, 1109, 1419
NciI CCSGG 1 cut(s) 1487
NdeII GATC 9 cut(s) 128, 253, 373, 914, 950, 1050, 1426, 1493, 1545
NlaIII CATG 7 cut(s) 31, 380, 460, 1555, 1567, 1648, 1703
NlaIV GGNNCC 7 cut(s) 280, 785, 786, 916, 1204, 1306, 1617
NmuCI GTSAC 2 cut(s) 589, 1399
NsbI TGCGCA 1 cut(s) 175
NsiI ATGCAT 1 cut(s) 1705
NspI RCATGY 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 1781
PaeR7I CTCGAG 2 cut(s) 548, 696
PagI TCATGA 2 cut(s) 376, 456
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PctI GAATGC 1 cut(s) 1730
PfeI GAWTC 3 cut(s) 203, 958, 1759
PkrI GCNGC 8 cut(s) 514, 793, 906, 1114, 1421, 1468, 1471, 1770
PleI GAGTC 3 cut(s) 715, 1205, 1565
PpsI GAGTC 3 cut(s) 715, 1205, 1565
PpuMI RGGWCCY 1 cut(s) 784
PshAI GACNNNNGTC 1 cut(s) 782
Psp5II RGGWCCY 1 cut(s) 784
Psp6I CCWGG 3 cut(s) 391, 500, 1245
PspFI CCCAGC 2 cut(s) 787, 971
PspGI CCWGG 3 cut(s) 391, 500, 1245
PspN4I GGNNCC 7 cut(s) 280, 785, 786, 916, 1204, 1306, 1617
PspPI GGNCC 3 cut(s) 295, 784, 1166
PspPPI RGGWCCY 1 cut(s) 784
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 3 cut(s) 914, 950, 1493
RsaI GTAC 5 cut(s) 934, 940, 989, 1631, 1643
RsaNI GTAC 5 cut(s) 933, 939, 988, 1630, 1642
RseI CAYNNNNRTG 2 cut(s) 26, 1781
SalI GTCGAC 1 cut(s) 1310
SaqAI TTAA 1 cut(s) 1683
SatI GCNGC 8 cut(s) 513, 792, 905, 1113, 1420, 1467, 1470, 1769
Sau3AI GATC 9 cut(s) 128, 253, 373, 914, 950, 1050, 1426, 1493, 1545
Sau96I GGNCC 3 cut(s) 295, 784, 1166
ScaI AGTACT 1 cut(s) 940
SchI GAGTC 3 cut(s) 716, 1206, 1565
ScrFI CCNGG 4 cut(s) 393, 502, 1247, 1487
SduI GDGCHC 1 cut(s) 1389
SfaNI GCATC 5 cut(s) 421, 829, 1481, 1673, 1712
SfcI CTRYAG 1 cut(s) 435
Sfr274I CTCGAG 2 cut(s) 548, 696
SinI GGWCC 3 cut(s) 295, 784, 1166
SlaI CTCGAG 2 cut(s) 548, 696
SmiMI CAYNNNNRTG 2 cut(s) 26, 1781
SmlI CTYRAG 8 cut(s) 70, 327, 416, 548, 696, 1388, 1407, 1719
SmoI CTYRAG 8 cut(s) 70, 327, 416, 548, 696, 1388, 1407, 1719
SpeI ACTAGT 1 cut(s) 935
Sse9I AATT 5 cut(s) 36, 57, 119, 1648, 1803
SsiI CCGC 7 cut(s) 50, 361, 399, 1373, 1383, 1466, 1469
SspMI CTAG 7 cut(s) 126, 272, 539, 629, 675, 809, 936
StyD4I CCNGG 4 cut(s) 391, 500, 1245, 1485
StyI CCWWGG 3 cut(s) 648, 918, 1598
TaaI ACNGT 7 cut(s) 307, 767, 1108, 1278, 1309, 1524, 1738
TaiI ACGT 5 cut(s) 495, 782, 1020, 1511, 1715
TaqI TCGA 9 cut(s) 447, 473, 549, 697, 1195, 1311, 1538, 1574, 1582
TaqII GACCGA 2 cut(s) 283, 1204
TasI AATT 5 cut(s) 36, 57, 119, 1648, 1803
TatI WGTACW 4 cut(s) 938, 987, 1629, 1641
TauI GCSGC 2 cut(s) 1469, 1472
TfiI GAWTC 3 cut(s) 203, 958, 1759
Tru1I TTAA 1 cut(s) 1683
Tru9I TTAA 1 cut(s) 1683
TscAI CASTG 6 cut(s) 310, 514, 667, 709, 897, 1783
TseFI GTSAC 2 cut(s) 589, 1399
TseI GCWGC 6 cut(s) 512, 791, 904, 1112, 1419, 1768
Tsp45I GTSAC 2 cut(s) 589, 1399
TspDTI ATGAA 5 cut(s) 1120, 1306, 1552, 1661, 1738
TspRI CASTG 6 cut(s) 310, 514, 667, 709, 897, 1783
VpaK11BI GGWCC 3 cut(s) 295, 784, 1166
XagI CCTNNNNNAGG 1 cut(s) 1675
XapI RAATTY 3 cut(s) 57, 119, 1648
XbaI TCTAGA 3 cut(s) 125, 538, 808
XceI RCATGY 1 cut(s) 31
XhoI CTCGAG 2 cut(s) 548, 696
XmiI GTMKAC 3 cut(s) 1221, 1311, 1715
XspI CTAG 7 cut(s) 126, 272, 539, 629, 675, 809, 936
ZrmI AGTACT 1 cut(s) 940
Zsp2I ATGCAT 1 cut(s) 1705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.