Rw4G013060

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011630
Physical Location & Seq
Reverse (-)
30565945 .. 30568713
2769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013060.1

Sequence Viewer

Length: 1800 bp
ATGCCTGTCTCTCACTCTGCCATAATTATGTTCTTGGCGGTGGAAATTTTATTCTTCTCAAGCCTATTATTAGCAAAAGCTTCTCCTTCACAATCCCATTTCTCCAAAATTTCTAGATCATCGGGAGGACTTTCCCATAAAGAAGCCATTTTATCTCTCTGCGCACATACCCCTCACCGAGCAACTTGTGAATCCACTCTCCTCTCGTCCTCCTCCTCATCATCATCTACAAGAGGCTCTGATCATCTCCCTCATACCCTAGAAGGTGCCTTTGTCCAGTCGGTCCATTCCACCGTTGCGAAAGCTCAGCACGCTCGAGCTCTCGCCTATAATCTCACTATTTCCCACCGCCAAAATGCCGATCATGATGTTCACTTCCCTGGTCGCGGTGTGAACGACTGCCTTGAGCTTCTCGATGATGCTATAGATATGCTCGACAATGTCATGATGACATCGTTGTCAAAACCTAACGATGACGACGTTCACACCTGGCTTAGTGCAGCTCTTACTAACCAAGAAACTTGTCTAGAGAGCCTCGAGAAAGACAAGTTCAAGACAGAGAAAAACATTATGGATGTCACGGCTCAAAATCTGAGCCAATACATAAGCAACTCCCTAGCACTTTTCGTGCTTACCATGGGTAGTAGCAGTGTCTCCTTCGCTAGTAGTTCGGAGCATTTACCTCGAGACACTGAGTCAAAGTCAAAGGAAGCTAAAAATGCAAACCCTAATAGAAGACTATTGGCATCGGACAGTAGTTTTCCGACGTGGGTCCGGGCCGCAGAGAGGAAGCTTCTAGAAGCTTCTGTTAGGGAGATAGAAGCAGATGCTGTGGTGGCTCAGGATGGGAGTGGGACGCACAAGAGCATTGGTGAGGCAGTGAAGGGGTTGCTGCTAATTGGAACCTTGGAGAGTAGTGGTACTAGTACTGGGGGGAGATCTGTGATTTACGTAAAAGCTGGGACATACCACGAGTACATAAACATACCCACGAAGCAAAAGAACGTGATGTTGATTGGTGATGGAGTGGGGAAAACTGTCATTGTGGGCAACAGGAACTCAGATGATGGCTGGACAACTTATAGGTCTGCTACTGTGGCTGCTATGGGTGATGGGTTCATCGCTCGTGATATAACATTTGTGAACGATGCCGGCCCTTCCAAGCACCAAGCAGTCGCACTTCGAGTCGGTGCCGACAAATCAGTCGTCCACCGATGTTCCATCATAGGCTACCAGGACACCCTCTACACTCACTCCAAGCGACAGTTCTACCGAGAAACCGACATCTATGGAACCGTCGACTTCATCTTCGGCAACTCAGCCGTGGTTTTCCAAACTTGCAACATCCACCCCCGAAAACCGCCCTCCAGCGGACTAAAGAACTTTGTCACAGCTCAAGGCCGAAGCAGCTCTAATCAGAACACAGGTATTTCAATCCACAACTGTAAGATAGCGGCGGCATCAGACCTCTTCCCGGTGAGATCCAGCTATGAGACGTATCTTGGGAGACCGTGGAAGCAGTATTCGAGAACGATCATCATGCAGTCTTTCATGGATGACTCGATAAATCGAGCAGGGTGGTCTCCTTGGTCTGGTGGGTCTGCACTTAACACATTGTACTATGGAGAGTACATGAATTCTGGGTCTGGGGCATCAACCTCAGGAAGGGTTAAATGGGGTGGATATCATGCATCACTTACGTCCACTCAAGCAGAGGCATTCACAGTTGGGGGTTTCATATCTGGGAATCTTTGGCTGCCTTCCACTGGGGTGTCTTTTAGTTCTGGACTAATTGGATAA

Protein Analysis

599

Amino Acids

64.67

Weight (kDa)

7.72

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 49 - 208 5.6e-26 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 276 - 583 6e-135 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 457, 1202
Acc16I TGCGCA 1 cut(s) 163
AccB1I GGYRCC 2 cut(s) 266, 1190
AccI GTMKAC 1 cut(s) 1299
AccII CGCG 1 cut(s) 387
AciI CCGC 8 cut(s) 38, 349, 387, 780, 1361, 1371, 1454, 1457
AclWI GGATC 1 cut(s) 1476
AcsI RAATTY 3 cut(s) 45, 108, 1636
AfaI GTAC 5 cut(s) 922, 928, 977, 1619, 1631
AfiI CCNNNNNNNGG 7 cut(s) 386, 786, 1370, 1474, 1592, 1665, 1766
AgsI TTSAA 2 cut(s) 553, 1434
AhdI GACNNNNNGTC 1 cut(s) 694
AhlI ACTAGT 1 cut(s) 923
AjiI CACGTC 1 cut(s) 768
AjnI CCWGG 3 cut(s) 379, 488, 1233
AjuI GAANNNNNNNTTGG 2 cut(s) 1250, 1282
AleI CACNNNNGTG 1 cut(s) 1769
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 6 cut(s) 13, 658, 681, 1487, 1501, 1587
AlwI GGATC 1 cut(s) 1476
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 3 cut(s) 315, 536, 684
AoxI GGCC 3 cut(s) 777, 1153, 1399
ApeKI GCWGC 5 cut(s) 500, 892, 1100, 1407, 1756
ApoI RAATTY 3 cut(s) 45, 108, 1636
ArsI GACNNNNNNTTYG 4 cut(s) 1191, 1223, 1292, 1324
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 4 cut(s) 283, 772, 777, 1154
AsuC2I CCSGG 2 cut(s) 776, 1475
AsuHPI GGTGA 5 cut(s) 167, 884, 1031, 1121, 1489
AvaI CYCGRG 3 cut(s) 315, 536, 684
AvaII GGWCC 2 cut(s) 283, 772
AxyI CCTNAGG 1 cut(s) 1660
BanI GGYRCC 2 cut(s) 266, 1190
BanII GRGCYC 1 cut(s) 322
BauI CACGAG 2 cut(s) 971, 1125
BbsI GAAGAC 1 cut(s) 742
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 5 cut(s) 512, 879, 1087, 1419, 1743
BccI CCATC 5 cut(s) 839, 1016, 1061, 1106, 1229
BceAI ACGGC 2 cut(s) 597, 1307
BcgI CGANNNNNNTGC 4 cut(s) 665, 699, 1522, 1556
BciT130I CCWGG 3 cut(s) 381, 490, 1235
BclI TGATCA 1 cut(s) 241
BcnI CCSGG 2 cut(s) 776, 1475
BcoDI GTCTC 6 cut(s) 13, 658, 681, 1487, 1501, 1587
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 7 cut(s) 114, 260, 527, 617, 663, 797, 924
BfmI CTRYAG 1 cut(s) 423
BglII AGATCT 1 cut(s) 938
BisI GCNGC 8 cut(s) 501, 780, 893, 1101, 1408, 1455, 1458, 1757
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 8 cut(s) 502, 781, 894, 1102, 1409, 1456, 1459, 1758
BmcAI AGTACT 1 cut(s) 928
Bme1390I CCNGG 5 cut(s) 381, 490, 776, 1235, 1475
Bme18I GGWCC 2 cut(s) 283, 772
BmeRI GACNNNNNGTC 1 cut(s) 694
BmeT110I CYCGRG 3 cut(s) 315, 536, 684
BmgBI CACGTC 1 cut(s) 768
BmgT120I GGNCC 4 cut(s) 283, 772, 777, 1154
BmiI GGNNCC 5 cut(s) 268, 773, 904, 1192, 1294
BmrFI CCNGG 5 cut(s) 381, 490, 776, 1235, 1475
BmrI ACTGGG 2 cut(s) 939, 1776
BmsI GCATC 7 cut(s) 409, 755, 817, 1138, 1469, 1661, 1700
BmuI ACTGGG 2 cut(s) 939, 1776
BoxI GACNNNNGTC 1 cut(s) 770
BpiI GAAGAC 1 cut(s) 742
BpmI CTGGAG 1 cut(s) 1351
Bpu10I CCTNAGC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 4 cut(s) 43, 425, 1380, 1692
BpuMI CCSGG 2 cut(s) 776, 1475
BsaAI YACGTR 1 cut(s) 952
BsaI GGTCTC 2 cut(s) 1501, 1587
BsaJI CCNNGG 6 cut(s) 379, 636, 906, 1323, 1511, 1586
BsaXI ACNNNNNCTCC 2 cut(s) 1238, 1268
Bsc4I CCNNNNNNNGG 7 cut(s) 386, 786, 1370, 1474, 1592, 1665, 1766
Bse118I RCCGGY 1 cut(s) 1151
Bse1I ACTGG 3 cut(s) 277, 934, 1771
Bse21I CCTNAGG 1 cut(s) 1660
BseBI CCWGG 3 cut(s) 381, 490, 1235
BseDI CCNNGG 6 cut(s) 379, 636, 906, 1323, 1511, 1586
BseGI GGATG 4 cut(s) 580, 850, 1344, 1561
BseLI CCNNNNNNNGG 7 cut(s) 386, 786, 1370, 1474, 1592, 1665, 1766
BseMII CTCAG 7 cut(s) 320, 584, 684, 854, 1074, 1332, 1674
BseNI ACTGG 3 cut(s) 277, 934, 1771
BseRI GAGGAG 3 cut(s) 191, 202, 205
BseXI GCAGC 5 cut(s) 512, 879, 1087, 1419, 1743
BseYI CCCAGC 1 cut(s) 959
BsgI GTGCAG 2 cut(s) 519, 1587
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 3 cut(s) 779, 1155, 1401
BshNI GGYRCC 2 cut(s) 266, 1190
BsiHKAI GWGCWC 1 cut(s) 322
BsiHKCI CYCGRG 3 cut(s) 315, 536, 684
BsiSI CCGG 3 cut(s) 775, 1152, 1475
BslFI GGGAC 2 cut(s) 868, 976
BslI CCNNNNNNNGG 7 cut(s) 386, 786, 1370, 1474, 1592, 1665, 1766
BsmAI GTCTC 6 cut(s) 13, 658, 681, 1487, 1501, 1587
BsmBI CGTCTC 1 cut(s) 1487
BsmFI GGGAC 2 cut(s) 868, 976
BsmI GAATGC 1 cut(s) 1718
BsnI GGCC 3 cut(s) 779, 1155, 1401
Bso31I GGTCTC 2 cut(s) 1501, 1587
BsoBI CYCGRG 3 cut(s) 315, 536, 684
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 6 cut(s) 116, 241, 361, 938, 1481, 1533
Bsp1720I GCTNAGC 1 cut(s) 306
Bsp19I CCATGG 1 cut(s) 636
BspACI CCGC 8 cut(s) 38, 349, 387, 780, 1361, 1371, 1454, 1457
BspANI GGCC 3 cut(s) 779, 1155, 1401
BspCNI CTCAG 7 cut(s) 319, 585, 685, 853, 1073, 1331, 1673
BspFNI CGCG 1 cut(s) 387
BspHI TCATGA 2 cut(s) 364, 444
BspLI GGNNCC 5 cut(s) 268, 773, 904, 1192, 1294
BspPI GGATC 1 cut(s) 1476
BspT107I GGYRCC 2 cut(s) 266, 1190
BspTNI GGTCTC 2 cut(s) 1501, 1587
BsrFI RCCGGY 1 cut(s) 1151
BsrI ACTGG 3 cut(s) 277, 934, 1771
BssAI RCCGGY 1 cut(s) 1151
BssECI CCNNGG 6 cut(s) 379, 636, 906, 1323, 1511, 1586
BssMI GATC 6 cut(s) 116, 241, 361, 938, 1481, 1533
BssSI CACGAG 2 cut(s) 971, 1125
BssT1I CCWWGG 3 cut(s) 636, 906, 1586
Bst2BI CACGAG 2 cut(s) 971, 1125
Bst2UI CCWGG 3 cut(s) 381, 490, 1235
Bst4CI ACNGT 9 cut(s) 295, 755, 1039, 1096, 1266, 1297, 1445, 1512, 1726
Bst6I CTCTTC 1 cut(s) 1475
BstBAI YACGTR 1 cut(s) 952
BstC8I GCNNGC 2 cut(s) 312, 1153
BstDEI CTNAG 8 cut(s) 306, 494, 593, 693, 840, 1060, 1318, 1660
BstDSI CCRYGG 3 cut(s) 636, 1323, 1511
BstENI CCTNNNNNAGG 1 cut(s) 1663
BstF5I GGATG 4 cut(s) 580, 850, 1344, 1561
BstFNI CGCG 1 cut(s) 387
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 6 cut(s) 119, 244, 364, 941, 1484, 1536
BstMAI GTCTC 6 cut(s) 13, 658, 681, 1487, 1501, 1587
BstMBI GATC 6 cut(s) 116, 241, 361, 938, 1481, 1533
BstMWI GCNNNNNNNGC 5 cut(s) 311, 719, 836, 1097, 1407
BstNI CCWGG 3 cut(s) 381, 490, 1235
BstPAI GACNNNNGTC 1 cut(s) 770
BstSCI CCNGG 5 cut(s) 379, 488, 774, 1233, 1473
BstSFI CTRYAG 1 cut(s) 423
BstSNI TACGTA 1 cut(s) 952
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 5 cut(s) 512, 879, 1087, 1419, 1743
BstV2I GAAGAC 1 cut(s) 742
BstX2I RGATCY 2 cut(s) 938, 1481
BstYI RGATCY 2 cut(s) 938, 1481
Bsu36I CCTNAGG 1 cut(s) 1660
BsuRI GGCC 3 cut(s) 779, 1155, 1401
BtgI CCRYGG 3 cut(s) 636, 1323, 1511
BtgZI GCGATG 1 cut(s) 1105
BtrI CACGTC 1 cut(s) 768
BtsCI GGATG 4 cut(s) 580, 850, 1344, 1561
BtsI GCAGTG 2 cut(s) 655, 885
BtsIMutI CAGTG 4 cut(s) 655, 690, 885, 1764
Cac8I GCNNGC 2 cut(s) 312, 1153
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 2 cut(s) 364, 444
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 1151
Cfr13I GGNCC 4 cut(s) 283, 772, 777, 1154
CseI GACGC 1 cut(s) 865
Csp6I GTAC 5 cut(s) 921, 927, 976, 1618, 1630
CviAII CATG 7 cut(s) 365, 445, 637, 1540, 1552, 1633, 1688
CviQI GTAC 5 cut(s) 921, 927, 976, 1618, 1630
DdeI CTNAG 8 cut(s) 306, 494, 593, 693, 840, 1060, 1318, 1660
DpnI GATC 6 cut(s) 118, 243, 363, 940, 1483, 1535
DpnII GATC 6 cut(s) 116, 241, 361, 938, 1481, 1533
DrdI GACNNNNNNGTC 2 cut(s) 457, 1202
DriI GACNNNNNGTC 1 cut(s) 694
DseDI GACNNNNNNGTC 2 cut(s) 457, 1202
Eam1104I CTCTTC 1 cut(s) 1475
Eam1105I GACNNNNNGTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 1475
Ecl136II GAGCTC 1 cut(s) 320
Eco105I TACGTA 1 cut(s) 952
Eco130I CCWWGG 3 cut(s) 636, 906, 1586
Eco24I GRGCYC 1 cut(s) 322
Eco31I GGTCTC 2 cut(s) 1501, 1587
Eco32I GATATC 1 cut(s) 1685
Eco47I GGWCC 2 cut(s) 283, 772
Eco53kI GAGCTC 1 cut(s) 320
Eco81I CCTNAGG 1 cut(s) 1660
Eco88I CYCGRG 3 cut(s) 315, 536, 684
EcoICRI GAGCTC 1 cut(s) 320
EcoNI CCTNNNNNAGG 1 cut(s) 1663
EcoRI GAATTC 1 cut(s) 1636
EcoRII CCWGG 3 cut(s) 379, 488, 1233
EcoRV GATATC 1 cut(s) 1685
EcoT14I CCWWGG 3 cut(s) 636, 906, 1586
EcoT22I ATGCAT 1 cut(s) 1693
EcoT38I GRGCYC 1 cut(s) 322
ErhI CCWWGG 3 cut(s) 636, 906, 1586
Esp3I CGTCTC 1 cut(s) 1487
FaeI CATG 7 cut(s) 368, 448, 640, 1543, 1555, 1636, 1691
FaqI GGGAC 2 cut(s) 868, 976
FatI CATG 7 cut(s) 364, 444, 636, 1539, 1551, 1632, 1687
FbaI TGATCA 1 cut(s) 241
FblI GTMKAC 1 cut(s) 1299
Fnu4HI GCNGC 8 cut(s) 501, 780, 893, 1101, 1408, 1455, 1458, 1757
FokI GGATG 4 cut(s) 587, 857, 1331, 1568
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 8 cut(s) 501, 780, 893, 1101, 1408, 1455, 1458, 1757
FspBI CTAG 7 cut(s) 114, 260, 527, 617, 663, 797, 924
FspI TGCGCA 1 cut(s) 163
GlaI GCGC 1 cut(s) 163
GluI GCNGC 8 cut(s) 501, 780, 893, 1101, 1408, 1455, 1458, 1757
GsaI CCCAGC 1 cut(s) 963
GsuI CTGGAG 1 cut(s) 1351
HaeIII GGCC 3 cut(s) 779, 1155, 1401
HapII CCGG 3 cut(s) 775, 1152, 1475
HgaI GACGC 1 cut(s) 865
HhaI GCGC 1 cut(s) 164
Hin1II CATG 7 cut(s) 368, 448, 640, 1543, 1555, 1636, 1691
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 1300
HindII GTYRAC 1 cut(s) 1300
HindIII AAGCTT 3 cut(s) 78, 791, 801
HinfI GANTC 5 cut(s) 191, 695, 1185, 1559, 1747
HpaII CCGG 3 cut(s) 775, 1152, 1475
HphI GGTGA 5 cut(s) 167, 884, 1031, 1121, 1489
Hpy166II GTNNAC 7 cut(s) 373, 394, 484, 1144, 1210, 1300, 1704
Hpy188I TCNGA 8 cut(s) 241, 594, 673, 751, 765, 1063, 1419, 1465
Hpy8I GTNNAC 7 cut(s) 373, 394, 484, 1144, 1210, 1300, 1704
Hpy99I CGWCG 3 cut(s) 482, 769, 1301
HpyAV CCTTC 7 cut(s) 96, 257, 667, 877, 1167, 1659, 1770
HpyCH4III ACNGT 9 cut(s) 295, 755, 1039, 1096, 1266, 1297, 1445, 1512, 1726
HpyCH4IV ACGT 6 cut(s) 480, 767, 951, 1005, 1496, 1700
HpyCH4V TGCA 6 cut(s) 500, 722, 1341, 1543, 1604, 1691
HpyF10VI GCNNNNNNNGC 5 cut(s) 311, 719, 836, 1097, 1407
HpyF3I CTNAG 8 cut(s) 306, 494, 593, 693, 840, 1060, 1318, 1660
HpySE526I ACGT 6 cut(s) 480, 767, 951, 1005, 1496, 1700
Hsp92II CATG 7 cut(s) 368, 448, 640, 1543, 1555, 1636, 1691
HspAI GCGC 1 cut(s) 162
KroI GCCGGC 1 cut(s) 1151
KroNI GCCGGC 1 cut(s) 1153
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 6 cut(s) 116, 241, 361, 938, 1481, 1533
LmnI GCTCC 1 cut(s) 673
Lsp1109I GCAGC 5 cut(s) 512, 879, 1087, 1419, 1743
LweI GCATC 7 cut(s) 409, 755, 817, 1138, 1469, 1661, 1700
MaeI CTAG 7 cut(s) 114, 260, 527, 617, 663, 797, 924
MaeII ACGT 6 cut(s) 480, 767, 951, 1005, 1496, 1700
MaeIII GTNAC 2 cut(s) 577, 1387
MalI GATC 6 cut(s) 118, 243, 363, 940, 1483, 1535
MboI GATC 6 cut(s) 116, 241, 361, 938, 1481, 1533
MboII GAAGA 4 cut(s) 46, 747, 1300, 1462
MflI RGATCY 2 cut(s) 938, 1481
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 6 cut(s) 24, 45, 108, 897, 1636, 1791
MlyI GAGTC 3 cut(s) 704, 1194, 1553
MmeI TCCRAC 1 cut(s) 788
Mph1103I ATGCAT 1 cut(s) 1693
MroNI GCCGGC 1 cut(s) 1151
MseI TTAA 2 cut(s) 1608, 1671
MslI CAYNNNNRTG 2 cut(s) 26, 1769
MspA1I CMGCKG 1 cut(s) 1371
MspI CCGG 3 cut(s) 775, 1152, 1475
MspR9I CCNGG 5 cut(s) 381, 490, 776, 1235, 1475
Mva1269I GAATGC 1 cut(s) 1718
MvaI CCWGG 3 cut(s) 381, 490, 1235
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 5 cut(s) 311, 719, 836, 1097, 1407
NaeI GCCGGC 1 cut(s) 1153
NciI CCSGG 2 cut(s) 776, 1475
NcoI CCATGG 1 cut(s) 636
NdeII GATC 6 cut(s) 116, 241, 361, 938, 1481, 1533
NgoMIV GCCGGC 1 cut(s) 1151
NlaIII CATG 7 cut(s) 368, 448, 640, 1543, 1555, 1636, 1691
NlaIV GGNNCC 5 cut(s) 268, 773, 904, 1192, 1294
NmuCI GTSAC 2 cut(s) 577, 1387
NsbI TGCGCA 1 cut(s) 163
NsiI ATGCAT 1 cut(s) 1693
OliI CACNNNNGTG 1 cut(s) 1769
PaeR7I CTCGAG 3 cut(s) 315, 536, 684
PagI TCATGA 2 cut(s) 364, 444
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PctI GAATGC 1 cut(s) 1718
PdiI GCCGGC 1 cut(s) 1153
PfeI GAWTC 2 cut(s) 191, 1747
PflFI GACNNNGTC 1 cut(s) 440
PkrI GCNGC 8 cut(s) 502, 781, 894, 1102, 1409, 1456, 1459, 1758
PleI GAGTC 3 cut(s) 703, 1193, 1553
PpsI GAGTC 3 cut(s) 703, 1193, 1553
Ppu21I YACGTR 1 cut(s) 952
PshAI GACNNNNGTC 1 cut(s) 770
Psp124BI GAGCTC 1 cut(s) 322
Psp6I CCWGG 3 cut(s) 379, 488, 1233
PspFI CCCAGC 1 cut(s) 959
PspGI CCWGG 3 cut(s) 379, 488, 1233
PspN4I GGNNCC 5 cut(s) 268, 773, 904, 1192, 1294
PspPI GGNCC 4 cut(s) 283, 772, 777, 1154
PspXI VCTCGAGB 1 cut(s) 315
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 2 cut(s) 938, 1481
PsyI GACNNNGTC 1 cut(s) 440
RsaI GTAC 5 cut(s) 922, 928, 977, 1619, 1631
RsaNI GTAC 5 cut(s) 921, 927, 976, 1618, 1630
RseI CAYNNNNRTG 2 cut(s) 26, 1769
SacI GAGCTC 1 cut(s) 322
SalI GTCGAC 1 cut(s) 1298
SaqAI TTAA 2 cut(s) 1608, 1671
SatI GCNGC 8 cut(s) 501, 780, 893, 1101, 1408, 1455, 1458, 1757
Sau3AI GATC 6 cut(s) 116, 241, 361, 938, 1481, 1533
Sau96I GGNCC 4 cut(s) 283, 772, 777, 1154
ScaI AGTACT 1 cut(s) 928
SchI GAGTC 3 cut(s) 704, 1194, 1553
ScrFI CCNGG 5 cut(s) 381, 490, 776, 1235, 1475
SduI GDGCHC 1 cut(s) 322
SfaNI GCATC 7 cut(s) 409, 755, 817, 1138, 1469, 1661, 1700
SfcI CTRYAG 1 cut(s) 423
Sfr274I CTCGAG 3 cut(s) 315, 536, 684
SinI GGWCC 2 cut(s) 283, 772
SlaI CTCGAG 3 cut(s) 315, 536, 684
SmiMI CAYNNNNRTG 2 cut(s) 26, 1769
SmlI CTYRAG 7 cut(s) 58, 315, 404, 536, 684, 1395, 1707
SmoI CTYRAG 7 cut(s) 58, 315, 404, 536, 684, 1395, 1707
SnaBI TACGTA 1 cut(s) 952
SpeI ACTAGT 1 cut(s) 923
Sse9I AATT 6 cut(s) 24, 45, 108, 897, 1636, 1791
SsiI CCGC 8 cut(s) 38, 349, 387, 780, 1361, 1371, 1454, 1457
SspMI CTAG 7 cut(s) 114, 260, 527, 617, 663, 797, 924
SstI GAGCTC 1 cut(s) 322
StyD4I CCNGG 5 cut(s) 379, 488, 774, 1233, 1473
StyI CCWWGG 3 cut(s) 636, 906, 1586
TaaI ACNGT 9 cut(s) 295, 755, 1039, 1096, 1266, 1297, 1445, 1512, 1726
TaiI ACGT 6 cut(s) 483, 770, 954, 1008, 1499, 1703
TaqII GACCGA 1 cut(s) 271
TasI AATT 6 cut(s) 24, 45, 108, 897, 1636, 1791
TatI WGTACW 4 cut(s) 926, 975, 1617, 1629
TauI GCSGC 3 cut(s) 782, 1457, 1460
TfiI GAWTC 2 cut(s) 191, 1747
Tru1I TTAA 2 cut(s) 1608, 1671
Tru9I TTAA 2 cut(s) 1608, 1671
TscAI CASTG 4 cut(s) 655, 697, 885, 1771
TseFI GTSAC 2 cut(s) 577, 1387
TseI GCWGC 5 cut(s) 500, 892, 1100, 1407, 1756
Tsp45I GTSAC 2 cut(s) 577, 1387
TspDTI ATGAA 5 cut(s) 1108, 1294, 1540, 1649, 1726
TspRI CASTG 4 cut(s) 655, 697, 885, 1771
Tth111I GACNNNGTC 1 cut(s) 440
VpaK11BI GGWCC 2 cut(s) 283, 772
XagI CCTNNNNNAGG 1 cut(s) 1663
XapI RAATTY 3 cut(s) 45, 108, 1636
XbaI TCTAGA 3 cut(s) 113, 526, 796
XhoI CTCGAG 3 cut(s) 315, 536, 684
XmiI GTMKAC 1 cut(s) 1299
XspI CTAG 7 cut(s) 114, 260, 527, 617, 663, 797, 924
ZrmI AGTACT 1 cut(s) 928
Zsp2I ATGCAT 1 cut(s) 1693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.