Rroxscaffold_5G00354340

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011630
Physical Location & Seq
Reverse (-)
32785259 .. 32788052
2794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00354340.1

Sequence Viewer

Length: 1593 bp
ATGTTCTTGGCGGTGGAAATTTTATTCTTCTCAAGTATGTTATTAGCAAAAGCTTCTCCTTCACAATCCCTTTTCTCCAAATTTTCTAGATCATCCGGAGGACTTTACCATAAAGAAGCCATTTTATCTCTCTGCGCACATACCCCTCACCAAGCAACTTGTGAATCCACTCTCCTCTCCTCCTCCTCCTCGTCATCATCTACAAGAGGTTCTGATCATCTCCCTAATACCCTAGAAGGTGCCTTTGTCCAGTCGGTCCATTCCACTGTTGCGAAAGCTCAGCACGCTCGAGCTCTAGCCTATAATCTCACTATTTCCCACCGCCAAAATGCCGATCATGATGTTCACTTCCCTGGTCGCGGTGTGAACGACTGCCTTGAGCTTCTCGATGATGCTATAGATATGCTCGACAATGTCATGATGACATCGTTGTCGAAACCTAACGATGACGACGTTCACACGTGGCTCAGTGCAGCTCTTACGAACCAAGAAACTTGTCTAGAGAGCCTCGAGAAAGACAAGTTCAAGACAGAGAAAAACATTATGGATGTCACGGCTCAAAATCTGAGCCAATACATAAGCAACTCCCTAGCACTTTTCGTGCTTACCATGGGTAGTAGCAGTGTCTCCTTCGCTAGTGGTTCGGAGCATTTACCTCGAGACACTGAGTCAAAGTCAAGGGAAGCTAAAAATGCAAACCCTAATAGAAGACTATTGGCATCGGACAGTAGTTTTCCGACGTGGGTCCCAGCCGCAGAGAGGAAGCTTCTCGAAGCTTCTGTTGGGGAGATAGAAGCAGATGCTGTGGTGGCTCAGGATGGGAGTGGGACGCACAAGAGCATTGGTGAGGCAGTGAAGGGGTTGCTGCTCATTGGATCCTTGGAGAGTAGTGCTGCTATGGGTGATGGGTTCATCGCTCGTGATATAACATTTGTGAACGATGCCGGCCCTTCCAAGCACCAAGCAGTCGCACTTCGAGTCGGTGCCGACAAATCGGTCGTCCACCGATGTTCCATCATAGGCTACCAGGACACCCTCTACACTCACTCCAAGCGACAGTTCTACCGAGAAACCGACATCTATGGAACCGTCGACTTCATCTTCGGCAACTCAGCCGTGGTCTTCCAAGCTTGCAACATCTACCCCCGAAAACCGCCCTCCAGCGGGCTCAAGAACTTTGTCACAGCTCAAGGCCGAAGCAGCCCTGATCAGAACACAGGCATTTCAATCCACAACTGTAAGATAGCGGCTGCATCAGACCTCTTCCCGGTGAGATCCAGCTACGAGACGTATCTTGGGAGACCGTGGAAGCAGTATTCGAGAACGATCATCATGCAGTCTTTCATGGATGACTCGATAAATCGAGCAGGGTGGTCTCCTTGGTCTGGTGGGTTTGCTCTTAACACATTGTACTATGGAGAGTACATGAATTCTGGGTCTGGGGCATCAACCTCAGGAAGGGTTAAATGGGGTGGATGTCATGCATCACTTACGTCCACTCAAGCACAAGCATTCACAGTCGGGGGTTTCATATCTGGGAATATTTGGCTGCCTTCCACTGGGGTGTCTTTTAGTTCTGGACTAATTGGATAA

Protein Analysis

530

Amino Acids

57.19

Weight (kDa)

6.38

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 40 - 199 6.7e-26 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 298 - 514 1.1e-105 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 430, 995
Acc16I TGCGCA 1 cut(s) 136
AccB1I GGYRCC 2 cut(s) 239, 983
AccI GTMKAC 1 cut(s) 1092
AccII CGCG 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 95
AciI CCGC 7 cut(s) 11, 322, 360, 753, 1154, 1164, 1247
AclWI GGATC 3 cut(s) 870, 883, 1269
AcsI RAATTY 3 cut(s) 18, 80, 1429
AcvI CACGTG 1 cut(s) 462
AfaI GTAC 2 cut(s) 1412, 1424
AfiI CCNNNNNNNGG 8 cut(s) 359, 759, 1163, 1164, 1267, 1385, 1458, 1559
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 2 cut(s) 526, 1227
AhdI GACNNNNNGTC 1 cut(s) 667
AjiI CACGTC 1 cut(s) 741
AjnI CCWGG 2 cut(s) 352, 1026
AjuI GAANNNNNNNTTGG 4 cut(s) 765, 797, 1043, 1075
AleI CACNNNNGTG 1 cut(s) 1562
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 5 cut(s) 631, 654, 1280, 1294, 1380
AlwI GGATC 3 cut(s) 870, 883, 1269
AlwNI CAGNNNCTG 1 cut(s) 803
Ama87I CYCGRG 3 cut(s) 288, 509, 657
Aor13HI TCCGGA 1 cut(s) 95
AoxI GGCC 2 cut(s) 946, 1192
ApeKI GCWGC 6 cut(s) 473, 865, 893, 1200, 1250, 1549
ApoI RAATTY 3 cut(s) 18, 80, 1429
ArsI GACNNNNNNTTYG 4 cut(s) 984, 1016, 1085, 1117
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 256, 745, 947
AsuC2I CCSGG 1 cut(s) 1268
AsuHPI GGTGA 4 cut(s) 140, 857, 914, 1282
AvaI CYCGRG 3 cut(s) 288, 509, 657
AvaII GGWCC 2 cut(s) 256, 745
AxyI CCTNAGG 1 cut(s) 1453
BamHI GGATCC 1 cut(s) 875
BanI GGYRCC 2 cut(s) 239, 983
BanII GRGCYC 2 cut(s) 295, 1170
BauI CACGAG 1 cut(s) 918
BbrPI CACGTG 1 cut(s) 462
BbsI GAAGAC 2 cut(s) 715, 1114
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 6 cut(s) 485, 852, 880, 1212, 1237, 1536
BccI CCATC 3 cut(s) 812, 899, 1022
BceAI ACGGC 2 cut(s) 570, 1100
BcgI CGANNNNNNTGC 4 cut(s) 638, 672, 1315, 1349
BciT130I CCWGG 2 cut(s) 354, 1028
BclI TGATCA 2 cut(s) 214, 1207
BcnI CCSGG 1 cut(s) 1268
BcoDI GTCTC 5 cut(s) 631, 654, 1280, 1294, 1380
BfaI CTAG 6 cut(s) 87, 233, 296, 500, 590, 636
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 8 cut(s) 474, 753, 866, 894, 1201, 1248, 1251, 1550
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 8 cut(s) 475, 754, 867, 895, 1202, 1249, 1252, 1551
Bme1390I CCNGG 3 cut(s) 354, 1028, 1268
Bme18I GGWCC 2 cut(s) 256, 745
BmeRI GACNNNNNGTC 1 cut(s) 667
BmeT110I CYCGRG 3 cut(s) 288, 509, 657
BmgBI CACGTC 1 cut(s) 741
BmgT120I GGNCC 3 cut(s) 256, 745, 947
BmiI GGNNCC 6 cut(s) 241, 746, 747, 877, 985, 1087
BmrFI CCNGG 3 cut(s) 354, 1028, 1268
BmrI ACTGGG 1 cut(s) 1569
BmsI GCATC 7 cut(s) 382, 728, 790, 931, 1262, 1454, 1493
BmuI ACTGGG 1 cut(s) 1569
BoxI GACNNNNGTC 1 cut(s) 743
BpiI GAAGAC 2 cut(s) 715, 1114
BpmI CTGGAG 1 cut(s) 1144
Bpu10I CCTNAGC 1 cut(s) 813
Bpu1102I GCTNAGC 1 cut(s) 279
BpuEI CTTGAG 5 cut(s) 16, 398, 1154, 1173, 1485
BpuMI CCSGG 1 cut(s) 1268
BsaAI YACGTR 1 cut(s) 462
BsaI GGTCTC 2 cut(s) 1294, 1380
BsaJI CCNNGG 6 cut(s) 352, 609, 879, 1116, 1304, 1379
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 1031, 1061
Bsc4I CCNNNNNNNGG 8 cut(s) 359, 759, 1163, 1164, 1267, 1385, 1458, 1559
Bse118I RCCGGY 1 cut(s) 944
Bse1I ACTGG 2 cut(s) 250, 1564
Bse21I CCTNAGG 1 cut(s) 1453
BseAI TCCGGA 1 cut(s) 95
BseBI CCWGG 2 cut(s) 354, 1028
BseDI CCNNGG 6 cut(s) 352, 609, 879, 1116, 1304, 1379
BseGI GGATG 5 cut(s) 92, 553, 823, 1354, 1481
BseLI CCNNNNNNNGG 8 cut(s) 359, 759, 1163, 1164, 1267, 1385, 1458, 1559
BseMII CTCAG 7 cut(s) 293, 481, 557, 657, 827, 1125, 1467
BseNI ACTGG 2 cut(s) 250, 1564
BseRI GAGGAG 5 cut(s) 164, 169, 172, 175, 178
BseXI GCAGC 6 cut(s) 485, 852, 880, 1212, 1237, 1536
BseYI CCCAGC 1 cut(s) 748
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 360
Bsh1285I CGRYCG 1 cut(s) 999
BshFI GGCC 2 cut(s) 948, 1194
BshNI GGYRCC 2 cut(s) 239, 983
BsiEI CGRYCG 1 cut(s) 999
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 3 cut(s) 288, 509, 657
BsiSI CCGG 3 cut(s) 96, 945, 1268
BslFI GGGAC 2 cut(s) 731, 841
BslI CCNNNNNNNGG 8 cut(s) 359, 759, 1163, 1164, 1267, 1385, 1458, 1559
BsmAI GTCTC 5 cut(s) 631, 654, 1280, 1294, 1380
BsmBI CGTCTC 1 cut(s) 1280
BsmFI GGGAC 2 cut(s) 731, 841
BsmI GAATGC 1 cut(s) 1511
BsnI GGCC 2 cut(s) 948, 1194
Bso31I GGTCTC 2 cut(s) 1294, 1380
BsoBI CYCGRG 3 cut(s) 288, 509, 657
Bsp1286I GDGCHC 2 cut(s) 295, 1170
Bsp13I TCCGGA 1 cut(s) 95
Bsp143I GATC 7 cut(s) 89, 214, 334, 875, 1207, 1274, 1326
Bsp1720I GCTNAGC 1 cut(s) 279
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 7 cut(s) 11, 322, 360, 753, 1154, 1164, 1247
BspANI GGCC 2 cut(s) 948, 1194
BspCNI CTCAG 7 cut(s) 292, 480, 558, 658, 826, 1124, 1466
BspEI TCCGGA 1 cut(s) 95
BspFNI CGCG 1 cut(s) 360
BspHI TCATGA 2 cut(s) 337, 417
BspLI GGNNCC 6 cut(s) 241, 746, 747, 877, 985, 1087
BspPI GGATC 3 cut(s) 870, 883, 1269
BspT107I GGYRCC 2 cut(s) 239, 983
BspTNI GGTCTC 2 cut(s) 1294, 1380
BsrFI RCCGGY 1 cut(s) 944
BsrI ACTGG 2 cut(s) 250, 1564
BssAI RCCGGY 1 cut(s) 944
BssECI CCNNGG 6 cut(s) 352, 609, 879, 1116, 1304, 1379
BssMI GATC 7 cut(s) 89, 214, 334, 875, 1207, 1274, 1326
BssSI CACGAG 1 cut(s) 918
BssT1I CCWWGG 3 cut(s) 609, 879, 1379
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 354, 1028
Bst4CI ACNGT 7 cut(s) 268, 728, 1059, 1090, 1238, 1305, 1519
Bst6I CTCTTC 1 cut(s) 1268
BstBAI YACGTR 1 cut(s) 462
BstC8I GCNNGC 4 cut(s) 285, 946, 1132, 1166
BstDEI CTNAG 7 cut(s) 279, 467, 566, 666, 813, 1111, 1453
BstDSI CCRYGG 3 cut(s) 609, 1116, 1304
BstENI CCTNNNNNAGG 1 cut(s) 1456
BstF5I GGATG 5 cut(s) 92, 553, 823, 1354, 1481
BstFNI CGCG 1 cut(s) 360
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 7 cut(s) 92, 217, 337, 878, 1210, 1277, 1329
BstMAI GTCTC 5 cut(s) 631, 654, 1280, 1294, 1380
BstMBI GATC 7 cut(s) 89, 214, 334, 875, 1207, 1274, 1326
BstMCI CGRYCG 1 cut(s) 999
BstMWI GCNNNNNNNGC 4 cut(s) 284, 692, 809, 1200
BstNI CCWGG 2 cut(s) 354, 1028
BstPAI GACNNNNGTC 1 cut(s) 743
BstSCI CCNGG 3 cut(s) 352, 1026, 1266
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 6 cut(s) 485, 852, 880, 1212, 1237, 1536
BstV2I GAAGAC 2 cut(s) 715, 1114
BstX2I RGATCY 2 cut(s) 875, 1274
BstYI RGATCY 2 cut(s) 875, 1274
Bsu36I CCTNAGG 1 cut(s) 1453
BsuRI GGCC 2 cut(s) 948, 1194
BtgI CCRYGG 3 cut(s) 609, 1116, 1304
BtgZI GCGATG 1 cut(s) 898
BtrI CACGTC 1 cut(s) 741
BtsCI GGATG 5 cut(s) 92, 553, 823, 1354, 1481
BtsI GCAGTG 2 cut(s) 628, 858
BtsIMutI CAGTG 6 cut(s) 264, 475, 628, 663, 858, 1557
Cac8I GCNNGC 4 cut(s) 285, 946, 1132, 1166
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 2 cut(s) 337, 417
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 944
Cfr13I GGNCC 3 cut(s) 256, 745, 947
CseI GACGC 1 cut(s) 838
Csp6I GTAC 2 cut(s) 1411, 1423
CviAII CATG 7 cut(s) 338, 418, 610, 1333, 1345, 1426, 1481
CviQI GTAC 2 cut(s) 1411, 1423
DdeI CTNAG 7 cut(s) 279, 467, 566, 666, 813, 1111, 1453
DpnI GATC 7 cut(s) 91, 216, 336, 877, 1209, 1276, 1328
DpnII GATC 7 cut(s) 89, 214, 334, 875, 1207, 1274, 1326
DrdI GACNNNNNNGTC 2 cut(s) 430, 995
DriI GACNNNNNGTC 1 cut(s) 667
DseDI GACNNNNNNGTC 2 cut(s) 430, 995
Eam1104I CTCTTC 1 cut(s) 1268
Eam1105I GACNNNNNGTC 1 cut(s) 667
EarI CTCTTC 1 cut(s) 1268
Ecl136II GAGCTC 1 cut(s) 293
Eco130I CCWWGG 3 cut(s) 609, 879, 1379
Eco24I GRGCYC 2 cut(s) 295, 1170
Eco31I GGTCTC 2 cut(s) 1294, 1380
Eco47I GGWCC 2 cut(s) 256, 745
Eco53kI GAGCTC 1 cut(s) 293
Eco72I CACGTG 1 cut(s) 462
Eco81I CCTNAGG 1 cut(s) 1453
Eco88I CYCGRG 3 cut(s) 288, 509, 657
EcoICRI GAGCTC 1 cut(s) 293
EcoNI CCTNNNNNAGG 1 cut(s) 1456
EcoO109I RGGNCCY 1 cut(s) 745
EcoRI GAATTC 1 cut(s) 1429
EcoRII CCWGG 2 cut(s) 352, 1026
EcoT14I CCWWGG 3 cut(s) 609, 879, 1379
EcoT22I ATGCAT 1 cut(s) 1486
EcoT38I GRGCYC 2 cut(s) 295, 1170
ErhI CCWWGG 3 cut(s) 609, 879, 1379
Esp3I CGTCTC 1 cut(s) 1280
FaeI CATG 7 cut(s) 341, 421, 613, 1336, 1348, 1429, 1484
FaqI GGGAC 2 cut(s) 731, 841
FatI CATG 7 cut(s) 337, 417, 609, 1332, 1344, 1425, 1480
FauI CCCGC 1 cut(s) 1157
FbaI TGATCA 2 cut(s) 214, 1207
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 8 cut(s) 474, 753, 866, 894, 1201, 1248, 1251, 1550
FokI GGATG 5 cut(s) 79, 560, 830, 1361, 1488
FriOI GRGCYC 2 cut(s) 295, 1170
Fsp4HI GCNGC 8 cut(s) 474, 753, 866, 894, 1201, 1248, 1251, 1550
FspBI CTAG 6 cut(s) 87, 233, 296, 500, 590, 636
FspI TGCGCA 1 cut(s) 136
GlaI GCGC 1 cut(s) 136
GluI GCNGC 8 cut(s) 474, 753, 866, 894, 1201, 1248, 1251, 1550
GsaI CCCAGC 1 cut(s) 752
GsuI CTGGAG 1 cut(s) 1144
HaeIII GGCC 2 cut(s) 948, 1194
HapII CCGG 3 cut(s) 96, 945, 1268
HgaI GACGC 1 cut(s) 838
HhaI GCGC 1 cut(s) 137
Hin1II CATG 7 cut(s) 341, 421, 613, 1336, 1348, 1429, 1484
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HindIII AAGCTT 4 cut(s) 51, 764, 774, 1128
HinfI GANTC 4 cut(s) 164, 668, 978, 1352
HpaII CCGG 3 cut(s) 96, 945, 1268
HphI GGTGA 4 cut(s) 140, 857, 914, 1282
Hpy166II GTNNAC 7 cut(s) 346, 367, 457, 937, 1003, 1093, 1497
Hpy188I TCNGA 7 cut(s) 214, 567, 646, 724, 738, 1212, 1258
Hpy8I GTNNAC 7 cut(s) 346, 367, 457, 937, 1003, 1093, 1497
Hpy99I CGWCG 3 cut(s) 455, 742, 1094
HpyAV CCTTC 7 cut(s) 69, 230, 640, 850, 960, 1452, 1563
HpyCH4III ACNGT 7 cut(s) 268, 728, 1059, 1090, 1238, 1305, 1519
HpyCH4IV ACGT 5 cut(s) 453, 461, 740, 1289, 1493
HpyCH4V TGCA 6 cut(s) 473, 695, 1134, 1253, 1336, 1484
HpyF10VI GCNNNNNNNGC 4 cut(s) 284, 692, 809, 1200
HpyF3I CTNAG 7 cut(s) 279, 467, 566, 666, 813, 1111, 1453
HpySE526I ACGT 5 cut(s) 453, 461, 740, 1289, 1493
Hsp92II CATG 7 cut(s) 341, 421, 613, 1336, 1348, 1429, 1484
HspAI GCGC 1 cut(s) 135
KflI GGGWCCC 1 cut(s) 745
Kpn2I TCCGGA 1 cut(s) 95
KroI GCCGGC 1 cut(s) 944
KroNI GCCGGC 1 cut(s) 946
Ksp22I TGATCA 2 cut(s) 214, 1207
Kzo9I GATC 7 cut(s) 89, 214, 334, 875, 1207, 1274, 1326
LmnI GCTCC 1 cut(s) 646
Lsp1109I GCAGC 6 cut(s) 485, 852, 880, 1212, 1237, 1536
LweI GCATC 7 cut(s) 382, 728, 790, 931, 1262, 1454, 1493
MaeI CTAG 6 cut(s) 87, 233, 296, 500, 590, 636
MaeII ACGT 5 cut(s) 453, 461, 740, 1289, 1493
MaeIII GTNAC 2 cut(s) 550, 1180
MalI GATC 7 cut(s) 91, 216, 336, 877, 1209, 1276, 1328
MboI GATC 7 cut(s) 89, 214, 334, 875, 1207, 1274, 1326
MboII GAAGA 5 cut(s) 19, 720, 1093, 1114, 1255
MflI RGATCY 2 cut(s) 875, 1274
MhlI GDGCHC 2 cut(s) 295, 1170
MluCI AATT 4 cut(s) 18, 80, 1429, 1584
MlyI GAGTC 3 cut(s) 677, 987, 1346
MmeI TCCRAC 1 cut(s) 761
Mph1103I ATGCAT 1 cut(s) 1486
MroI TCCGGA 1 cut(s) 95
MroNI GCCGGC 1 cut(s) 944
MseI TTAA 2 cut(s) 1401, 1464
MslI CAYNNNNRTG 1 cut(s) 1562
MspA1I CMGCKG 1 cut(s) 1164
MspI CCGG 3 cut(s) 96, 945, 1268
MspR9I CCNGG 3 cut(s) 354, 1028, 1268
Mva1269I GAATGC 1 cut(s) 1511
MvaI CCWGG 2 cut(s) 354, 1028
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 4 cut(s) 284, 692, 809, 1200
NaeI GCCGGC 1 cut(s) 946
NciI CCSGG 1 cut(s) 1268
NcoI CCATGG 1 cut(s) 609
NdeII GATC 7 cut(s) 89, 214, 334, 875, 1207, 1274, 1326
NgoMIV GCCGGC 1 cut(s) 944
NlaIII CATG 7 cut(s) 341, 421, 613, 1336, 1348, 1429, 1484
NlaIV GGNNCC 6 cut(s) 241, 746, 747, 877, 985, 1087
NmuCI GTSAC 2 cut(s) 550, 1180
NsbI TGCGCA 1 cut(s) 136
NsiI ATGCAT 1 cut(s) 1486
OliI CACNNNNGTG 1 cut(s) 1562
PaeR7I CTCGAG 3 cut(s) 288, 509, 657
PagI TCATGA 2 cut(s) 337, 417
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PctI GAATGC 1 cut(s) 1511
PdiI GCCGGC 1 cut(s) 946
PfeI GAWTC 1 cut(s) 164
PflFI GACNNNGTC 1 cut(s) 413
PkrI GCNGC 8 cut(s) 475, 754, 867, 895, 1202, 1249, 1252, 1551
PleI GAGTC 3 cut(s) 676, 986, 1346
PmaCI CACGTG 1 cut(s) 462
PmlI CACGTG 1 cut(s) 462
PpsI GAGTC 3 cut(s) 676, 986, 1346
Ppu21I YACGTR 1 cut(s) 462
PpuMI RGGWCCY 1 cut(s) 745
PshAI GACNNNNGTC 1 cut(s) 743
Psp124BI GAGCTC 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 745
Psp6I CCWGG 2 cut(s) 352, 1026
PspCI CACGTG 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 748
PspGI CCWGG 2 cut(s) 352, 1026
PspN4I GGNNCC 6 cut(s) 241, 746, 747, 877, 985, 1087
PspPI GGNCC 3 cut(s) 256, 745, 947
PspPPI RGGWCCY 1 cut(s) 745
PspXI VCTCGAGB 1 cut(s) 288
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 2 cut(s) 875, 1274
PsyI GACNNNGTC 1 cut(s) 413
RsaI GTAC 2 cut(s) 1412, 1424
RsaNI GTAC 2 cut(s) 1411, 1423
RseI CAYNNNNRTG 1 cut(s) 1562
SacI GAGCTC 1 cut(s) 295
SalI GTCGAC 1 cut(s) 1091
SaqAI TTAA 2 cut(s) 1401, 1464
SatI GCNGC 8 cut(s) 474, 753, 866, 894, 1201, 1248, 1251, 1550
Sau3AI GATC 7 cut(s) 89, 214, 334, 875, 1207, 1274, 1326
Sau96I GGNCC 3 cut(s) 256, 745, 947
SchI GAGTC 3 cut(s) 677, 987, 1346
ScrFI CCNGG 3 cut(s) 354, 1028, 1268
SduI GDGCHC 2 cut(s) 295, 1170
SfaNI GCATC 7 cut(s) 382, 728, 790, 931, 1262, 1454, 1493
SfcI CTRYAG 1 cut(s) 396
Sfr274I CTCGAG 3 cut(s) 288, 509, 657
SinI GGWCC 2 cut(s) 256, 745
SlaI CTCGAG 3 cut(s) 288, 509, 657
SmiMI CAYNNNNRTG 1 cut(s) 1562
SmlI CTYRAG 8 cut(s) 31, 288, 377, 509, 657, 1169, 1188, 1500
SmoI CTYRAG 8 cut(s) 31, 288, 377, 509, 657, 1169, 1188, 1500
Sse9I AATT 4 cut(s) 18, 80, 1429, 1584
SsiI CCGC 7 cut(s) 11, 322, 360, 753, 1154, 1164, 1247
SspI AATATT 1 cut(s) 1543
SspMI CTAG 6 cut(s) 87, 233, 296, 500, 590, 636
SstI GAGCTC 1 cut(s) 295
StyD4I CCNGG 3 cut(s) 352, 1026, 1266
StyI CCWWGG 3 cut(s) 609, 879, 1379
TaaI ACNGT 7 cut(s) 268, 728, 1059, 1090, 1238, 1305, 1519
TaiI ACGT 5 cut(s) 456, 464, 743, 1292, 1496
TaqII GACCGA 2 cut(s) 244, 985
TasI AATT 4 cut(s) 18, 80, 1429, 1584
TatI WGTACW 2 cut(s) 1410, 1422
TauI GCSGC 2 cut(s) 755, 1250
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 2 cut(s) 1401, 1464
Tru9I TTAA 2 cut(s) 1401, 1464
TscAI CASTG 6 cut(s) 271, 475, 628, 670, 858, 1564
TseFI GTSAC 2 cut(s) 550, 1180
TseI GCWGC 6 cut(s) 473, 865, 893, 1200, 1250, 1549
Tsp45I GTSAC 2 cut(s) 550, 1180
TspDTI ATGAA 5 cut(s) 901, 1087, 1333, 1442, 1519
TspRI CASTG 6 cut(s) 271, 475, 628, 670, 858, 1564
Tth111I GACNNNGTC 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 256, 745
XagI CCTNNNNNAGG 1 cut(s) 1456
XapI RAATTY 3 cut(s) 18, 80, 1429
XbaI TCTAGA 2 cut(s) 86, 499
XhoI CTCGAG 3 cut(s) 288, 509, 657
XmiI GTMKAC 1 cut(s) 1092
XspI CTAG 6 cut(s) 87, 233, 296, 500, 590, 636
Zsp2I ATGCAT 1 cut(s) 1486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.