Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00782

Orthogroup ID

OG0011630

Physical Location & Seq

Genomic Coordinates Reverse (-)

5134 .. 8201

Gene Structure 3068 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G017480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGCCTGTCTCTCACTCTGCCATAATTATGTTCTTGGCGGTGGAAATTTTATTCTTCTCAAGCCTATTATTAGCAAAAGCTTCTCCTTCACAATCCCATTTCTCCAAATTTTCTAGATCATCCGGAGGACTTTCCCATAAAGAAGCCATTTTATCTCTCTGCGCACATACCCCTCACCGAGCAACTTGTGAATCCACTCTCCTCTCGTCCTCCTCCTCATCATCATCTACAAGAGGCTCTGATCATCTCCCTCATACCCTAGAAGGTGCCTTTGTCCAGTCGGTCCATTCCACCGTTGCGAAAGCTCAGCACGCTCGAGCTCTCGCCTATAATCTCACTATTTCCCACCGCCAAAATGCCGATCATGATGTTCACTTCCCTGGTCGCGGTGTGAACGACTGCCTTGAGCTTCTCGATGATGCTATAGATATGCTCGACAATGTCATGATGACATCGTTGTCAAAACCTAACGATGACGACGTTCACACCTGGCTTAGTGCAGCTCTTACTAACCAAGAAACTTGTCTAGAGAGCCTCGAGAAAGACAAGTTCAAGACAGAGAAAAACATTATGGATGTCACGGCTAAAAATCTGAGCCAATACATAAGCAACTCCCTAGCACTTTTCGTGCTTACCATGGGTAGTAGCAGTGTCTCCTTCGCTAGTAGTTCGGAGCATTTACCTCGAGACACTGAGTCAAAGTCAAAGGAAGCTAAAAATGCAAACCCTAATAGAAGACTATTGGCATCGGACAGTAGTTTTCCGACGTGGGTCCGGGCCGCAGAGAGGAAGCTTCTAGAAGCTTCTGTTAGGGAGATAGAAGCAGATGCTGTGGTGGCTCAGGATGGGAGTGGGACGCACAAGAGCATTGGTGAGGCAGTGAAGGGGTTGCTGCTAATTGGAACCTTGGAGAGTAGTGGTACTAGTACTGGGGGGAGATCTGTGATTTACGTAAAAGCTGGGACATACCACGAGTACATAAACATACCCACGAAGCAAAAGAACGTGATGTTGATTGGTGATGGAGTGGGGAAAACTATCATTGTGGGCAACAGGAACTCAGACGATGGCTGGACAACTTATAAGTCTGCTACTGTGGCTGCTATGGGTGATGGGTTCATCGCTCGTGATATAACATTTGTGAACGATGCCGGCCCTTCCAAGCACCAAGCAGTCGCACTTCGAGTCGGTGCCGACAAATCAGTCGTCCACCGATGTTCCATCATAGGCTACCAGGACACCCTCTACACTCACTCCAAGCGACAGTTCTACCGAGAAACCGACATCTATGGAACCGTCGACTTCATCTTCGGCAACTCAGCCGTGGTTTTCCAAACTTGCAACATCCACCCCCGAAAACCGCCCTCCAGCGGACTAAAGAACTTTGTCACAGCTCAAGGCCGAAGCAGCTCTAATCAGAACACAGGTATTTCAATCCACAACTGTAAGATAGCGGCGGCATCAGACCTCTTCCCGGTGAGATCCAGCTATGAGACGTATCTTGGGAGACCGTGGAAGCAGTATTCGAGAACGATCATCATGCAGTCTTTCATGGATGACTCGATAAATCGAGCAGGGTGGTCTCCTTGGTCTGGTGGGTCTGCACTTAACACATTGTACTATGGAGAGTACATGAATTCTGGGTCTGGGGCATCAACCTCAGGAAGGGTTAAATGGGGTGGATATCATGCATCACTTACGTCCACTCAAGCAGAGGCATTCACAGTTGGGGGTTTCATATCTGGGAATCTTTGGCTGCCTTCCACTGGGGTGTCTTTTAGTTCTGGACTAATTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

599

Amino Acids

64.69

Weight (kDa)

8.07

Isoelectric Point (pI)

35.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	49 - 208	4.5e-26	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	276 - 583	6e-135	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43050 AT3G59010
fragaria_vesca	FvH4_4g12140
malus_domestica	MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica	Prupe.1G178400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0409791
rosa_laevigata	RLG00000008522
rosa_multiflora	Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354340
rosa_rugosa	Rorug04G0096600
rosa_samantha	Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana	Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1083
AasI	GACNNNNNNGTC	2 cut(s)	457, 1202
Acc16I	TGCGCA	1 cut(s)	163
AccB1I	GGYRCC	2 cut(s)	266, 1190
AccI	GTMKAC	1 cut(s)	1299
AccII	CGCG	1 cut(s)	387
AccIII	TCCGGA	1 cut(s)	122
AciI	CCGC	8 cut(s)	38, 349, 387, 780, 1361, 1371, 1454, 1457
AclWI	GGATC	1 cut(s)	1476
AcsI	RAATTY	3 cut(s)	45, 107, 1636
AfaI	GTAC	5 cut(s)	922, 928, 977, 1619, 1631
AfiI	CCNNNNNNNGG	7 cut(s)	386, 786, 1370, 1474, 1592, 1665, 1766
AgsI	TTSAA	2 cut(s)	553, 1434
AhdI	GACNNNNNGTC	1 cut(s)	694
AhlI	ACTAGT	1 cut(s)	923
AjiI	CACGTC	1 cut(s)	768
AjnI	CCWGG	3 cut(s)	379, 488, 1233
AjuI	GAANNNNNNNTTGG	2 cut(s)	1250, 1282
AleI	CACNNNNGTG	1 cut(s)	1769
Alw21I	GWGCWC	1 cut(s)	322
Alw26I	GTCTC	6 cut(s)	13, 658, 681, 1487, 1501, 1587
AlwI	GGATC	1 cut(s)	1476
AlwNI	CAGNNNCTG	1 cut(s)	830
Ama87I	CYCGRG	3 cut(s)	315, 536, 684
Aor13HI	TCCGGA	1 cut(s)	122
AoxI	GGCC	3 cut(s)	777, 1153, 1399
ApeKI	GCWGC	5 cut(s)	500, 892, 1100, 1407, 1756
ApoI	RAATTY	3 cut(s)	45, 107, 1636
ArsI	GACNNNNNNTTYG	4 cut(s)	1191, 1223, 1292, 1324
AspLEI	GCGC	1 cut(s)	164
AspS9I	GGNCC	4 cut(s)	283, 772, 777, 1154
AsuC2I	CCSGG	2 cut(s)	776, 1475
AsuHPI	GGTGA	5 cut(s)	167, 884, 1031, 1121, 1489
AvaI	CYCGRG	3 cut(s)	315, 536, 684
AvaII	GGWCC	2 cut(s)	283, 772
AxyI	CCTNAGG	1 cut(s)	1660
BanI	GGYRCC	2 cut(s)	266, 1190
BanII	GRGCYC	1 cut(s)	322
BauI	CACGAG	2 cut(s)	971, 1125
BbsI	GAAGAC	1 cut(s)	742
Bbv12I	GWGCWC	1 cut(s)	322
BbvI	GCAGC	5 cut(s)	512, 879, 1087, 1419, 1743
BccI	CCATC	5 cut(s)	839, 1016, 1061, 1106, 1229
BceAI	ACGGC	2 cut(s)	597, 1307
BcgI	CGANNNNNNTGC	4 cut(s)	665, 699, 1522, 1556
BciT130I	CCWGG	3 cut(s)	381, 490, 1235
BclI	TGATCA	1 cut(s)	241
BcnI	CCSGG	2 cut(s)	776, 1475
BcoDI	GTCTC	6 cut(s)	13, 658, 681, 1487, 1501, 1587
BcuI	ACTAGT	1 cut(s)	923
BfaI	CTAG	7 cut(s)	114, 260, 527, 617, 663, 797, 924
BfmI	CTRYAG	1 cut(s)	423
BglII	AGATCT	1 cut(s)	938
BisI	GCNGC	8 cut(s)	501, 780, 893, 1101, 1408, 1455, 1458, 1757
BlpI	GCTNAGC	1 cut(s)	306
BlsI	GCNGC	8 cut(s)	502, 781, 894, 1102, 1409, 1456, 1459, 1758
BmcAI	AGTACT	1 cut(s)	928
Bme1390I	CCNGG	5 cut(s)	381, 490, 776, 1235, 1475
Bme18I	GGWCC	2 cut(s)	283, 772
BmeRI	GACNNNNNGTC	1 cut(s)	694
BmeT110I	CYCGRG	3 cut(s)	315, 536, 684
BmgBI	CACGTC	1 cut(s)	768
BmgT120I	GGNCC	4 cut(s)	283, 772, 777, 1154
BmiI	GGNNCC	5 cut(s)	268, 773, 904, 1192, 1294
BmrFI	CCNGG	5 cut(s)	381, 490, 776, 1235, 1475
BmrI	ACTGGG	2 cut(s)	939, 1776
BmsI	GCATC	7 cut(s)	409, 755, 817, 1138, 1469, 1661, 1700
BmuI	ACTGGG	2 cut(s)	939, 1776
BoxI	GACNNNNGTC	1 cut(s)	770
BpiI	GAAGAC	1 cut(s)	742
BpmI	CTGGAG	1 cut(s)	1351
Bpu10I	CCTNAGC	1 cut(s)	840
Bpu1102I	GCTNAGC	1 cut(s)	306
BpuEI	CTTGAG	4 cut(s)	43, 425, 1380, 1692
BpuMI	CCSGG	2 cut(s)	776, 1475
BsaAI	YACGTR	1 cut(s)	952
BsaI	GGTCTC	2 cut(s)	1501, 1587
BsaJI	CCNNGG	6 cut(s)	379, 636, 906, 1323, 1511, 1586
BsaWI	WCCGGW	1 cut(s)	122
BsaXI	ACNNNNNCTCC	2 cut(s)	1238, 1268
Bsc4I	CCNNNNNNNGG	7 cut(s)	386, 786, 1370, 1474, 1592, 1665, 1766
Bse118I	RCCGGY	1 cut(s)	1151
Bse1I	ACTGG	3 cut(s)	277, 934, 1771
Bse21I	CCTNAGG	1 cut(s)	1660
BseAI	TCCGGA	1 cut(s)	122
BseBI	CCWGG	3 cut(s)	381, 490, 1235
BseDI	CCNNGG	6 cut(s)	379, 636, 906, 1323, 1511, 1586
BseGI	GGATG	5 cut(s)	119, 580, 850, 1344, 1561
BseLI	CCNNNNNNNGG	7 cut(s)	386, 786, 1370, 1474, 1592, 1665, 1766
BseMII	CTCAG	7 cut(s)	320, 584, 684, 854, 1074, 1332, 1674
BseNI	ACTGG	3 cut(s)	277, 934, 1771
BseRI	GAGGAG	3 cut(s)	191, 202, 205
BseXI	GCAGC	5 cut(s)	512, 879, 1087, 1419, 1743
BseYI	CCCAGC	1 cut(s)	959
BsgI	GTGCAG	2 cut(s)	519, 1587
Bsh1236I	CGCG	1 cut(s)	387
BshFI	GGCC	3 cut(s)	779, 1155, 1401
BshNI	GGYRCC	2 cut(s)	266, 1190
BsiHKAI	GWGCWC	1 cut(s)	322
BsiHKCI	CYCGRG	3 cut(s)	315, 536, 684
BsiSI	CCGG	4 cut(s)	123, 775, 1152, 1475
BslFI	GGGAC	2 cut(s)	868, 976
BslI	CCNNNNNNNGG	7 cut(s)	386, 786, 1370, 1474, 1592, 1665, 1766
BsmAI	GTCTC	6 cut(s)	13, 658, 681, 1487, 1501, 1587
BsmBI	CGTCTC	1 cut(s)	1487
BsmFI	GGGAC	2 cut(s)	868, 976
BsmI	GAATGC	1 cut(s)	1718
BsnI	GGCC	3 cut(s)	779, 1155, 1401
Bso31I	GGTCTC	2 cut(s)	1501, 1587
BsoBI	CYCGRG	3 cut(s)	315, 536, 684
Bsp1286I	GDGCHC	1 cut(s)	322
Bsp13I	TCCGGA	1 cut(s)	122
Bsp143I	GATC	6 cut(s)	116, 241, 361, 938, 1481, 1533
Bsp1720I	GCTNAGC	1 cut(s)	306
Bsp19I	CCATGG	1 cut(s)	636
BspACI	CCGC	8 cut(s)	38, 349, 387, 780, 1361, 1371, 1454, 1457
BspANI	GGCC	3 cut(s)	779, 1155, 1401
BspCNI	CTCAG	7 cut(s)	319, 585, 685, 853, 1073, 1331, 1673
BspEI	TCCGGA	1 cut(s)	122
BspFNI	CGCG	1 cut(s)	387
BspHI	TCATGA	2 cut(s)	364, 444
BspLI	GGNNCC	5 cut(s)	268, 773, 904, 1192, 1294
BspPI	GGATC	1 cut(s)	1476
BspT107I	GGYRCC	2 cut(s)	266, 1190
BspTNI	GGTCTC	2 cut(s)	1501, 1587
BsrFI	RCCGGY	1 cut(s)	1151
BsrI	ACTGG	3 cut(s)	277, 934, 1771
BssAI	RCCGGY	1 cut(s)	1151
BssECI	CCNNGG	6 cut(s)	379, 636, 906, 1323, 1511, 1586
BssMI	GATC	6 cut(s)	116, 241, 361, 938, 1481, 1533
BssSI	CACGAG	2 cut(s)	971, 1125
BssT1I	CCWWGG	3 cut(s)	636, 906, 1586
Bst2BI	CACGAG	2 cut(s)	971, 1125
Bst2UI	CCWGG	3 cut(s)	381, 490, 1235
Bst4CI	ACNGT	8 cut(s)	295, 755, 1096, 1266, 1297, 1445, 1512, 1726
Bst6I	CTCTTC	1 cut(s)	1475
BstBAI	YACGTR	1 cut(s)	952
BstC8I	GCNNGC	2 cut(s)	312, 1153
BstDEI	CTNAG	8 cut(s)	306, 494, 593, 693, 840, 1060, 1318, 1660
BstDSI	CCRYGG	3 cut(s)	636, 1323, 1511
BstENI	CCTNNNNNAGG	1 cut(s)	1663
BstF5I	GGATG	5 cut(s)	119, 580, 850, 1344, 1561
BstFNI	CGCG	1 cut(s)	387
BstHHI	GCGC	1 cut(s)	164
BstKTI	GATC	6 cut(s)	119, 244, 364, 941, 1484, 1536
BstMAI	GTCTC	6 cut(s)	13, 658, 681, 1487, 1501, 1587
BstMBI	GATC	6 cut(s)	116, 241, 361, 938, 1481, 1533
BstMWI	GCNNNNNNNGC	5 cut(s)	311, 719, 836, 1097, 1407
BstNI	CCWGG	3 cut(s)	381, 490, 1235
BstPAI	GACNNNNGTC	1 cut(s)	770
BstSCI	CCNGG	5 cut(s)	379, 488, 774, 1233, 1473
BstSFI	CTRYAG	1 cut(s)	423
BstSNI	TACGTA	1 cut(s)	952
BstUI	CGCG	1 cut(s)	387
BstV1I	GCAGC	5 cut(s)	512, 879, 1087, 1419, 1743
BstV2I	GAAGAC	1 cut(s)	742
BstX2I	RGATCY	2 cut(s)	938, 1481
BstYI	RGATCY	2 cut(s)	938, 1481
Bsu36I	CCTNAGG	1 cut(s)	1660
BsuRI	GGCC	3 cut(s)	779, 1155, 1401
BtgI	CCRYGG	3 cut(s)	636, 1323, 1511
BtgZI	GCGATG	1 cut(s)	1105
BtrI	CACGTC	1 cut(s)	768
BtsCI	GGATG	5 cut(s)	119, 580, 850, 1344, 1561
BtsI	GCAGTG	2 cut(s)	655, 885
BtsIMutI	CAGTG	4 cut(s)	655, 690, 885, 1764
Cac8I	GCNNGC	2 cut(s)	312, 1153
CaiI	CAGNNNCTG	1 cut(s)	830
CciI	TCATGA	2 cut(s)	364, 444
CfoI	GCGC	1 cut(s)	164
Cfr10I	RCCGGY	1 cut(s)	1151
Cfr13I	GGNCC	4 cut(s)	283, 772, 777, 1154
CseI	GACGC	1 cut(s)	865
Csp6I	GTAC	5 cut(s)	921, 927, 976, 1618, 1630
CviAII	CATG	7 cut(s)	365, 445, 637, 1540, 1552, 1633, 1688
CviQI	GTAC	5 cut(s)	921, 927, 976, 1618, 1630
DdeI	CTNAG	8 cut(s)	306, 494, 593, 693, 840, 1060, 1318, 1660
DpnI	GATC	6 cut(s)	118, 243, 363, 940, 1483, 1535
DpnII	GATC	6 cut(s)	116, 241, 361, 938, 1481, 1533
DrdI	GACNNNNNNGTC	2 cut(s)	457, 1202
DriI	GACNNNNNGTC	1 cut(s)	694
DseDI	GACNNNNNNGTC	2 cut(s)	457, 1202
Eam1104I	CTCTTC	1 cut(s)	1475
Eam1105I	GACNNNNNGTC	1 cut(s)	694
EarI	CTCTTC	1 cut(s)	1475
Ecl136II	GAGCTC	1 cut(s)	320
Eco105I	TACGTA	1 cut(s)	952
Eco130I	CCWWGG	3 cut(s)	636, 906, 1586
Eco24I	GRGCYC	1 cut(s)	322
Eco31I	GGTCTC	2 cut(s)	1501, 1587
Eco32I	GATATC	1 cut(s)	1685
Eco47I	GGWCC	2 cut(s)	283, 772
Eco53kI	GAGCTC	1 cut(s)	320
Eco81I	CCTNAGG	1 cut(s)	1660
Eco88I	CYCGRG	3 cut(s)	315, 536, 684
EcoICRI	GAGCTC	1 cut(s)	320
EcoNI	CCTNNNNNAGG	1 cut(s)	1663
EcoRI	GAATTC	1 cut(s)	1636
EcoRII	CCWGG	3 cut(s)	379, 488, 1233
EcoRV	GATATC	1 cut(s)	1685
EcoT14I	CCWWGG	3 cut(s)	636, 906, 1586
EcoT22I	ATGCAT	1 cut(s)	1693
EcoT38I	GRGCYC	1 cut(s)	322
ErhI	CCWWGG	3 cut(s)	636, 906, 1586
Esp3I	CGTCTC	1 cut(s)	1487
FaeI	CATG	7 cut(s)	368, 448, 640, 1543, 1555, 1636, 1691
FaqI	GGGAC	2 cut(s)	868, 976
FatI	CATG	7 cut(s)	364, 444, 636, 1539, 1551, 1632, 1687
FbaI	TGATCA	1 cut(s)	241
FblI	GTMKAC	1 cut(s)	1299
Fnu4HI	GCNGC	8 cut(s)	501, 780, 893, 1101, 1408, 1455, 1458, 1757
FokI	GGATG	5 cut(s)	106, 587, 857, 1331, 1568
FriOI	GRGCYC	1 cut(s)	322
Fsp4HI	GCNGC	8 cut(s)	501, 780, 893, 1101, 1408, 1455, 1458, 1757
FspBI	CTAG	7 cut(s)	114, 260, 527, 617, 663, 797, 924
FspI	TGCGCA	1 cut(s)	163
GlaI	GCGC	1 cut(s)	163
GluI	GCNGC	8 cut(s)	501, 780, 893, 1101, 1408, 1455, 1458, 1757
GsaI	CCCAGC	1 cut(s)	963
GsuI	CTGGAG	1 cut(s)	1351
HaeIII	GGCC	3 cut(s)	779, 1155, 1401
HapII	CCGG	4 cut(s)	123, 775, 1152, 1475
HgaI	GACGC	1 cut(s)	865
HhaI	GCGC	1 cut(s)	164
Hin1II	CATG	7 cut(s)	368, 448, 640, 1543, 1555, 1636, 1691
Hin6I	GCGC	1 cut(s)	162
HinP1I	GCGC	1 cut(s)	162
HincII	GTYRAC	1 cut(s)	1300
HindII	GTYRAC	1 cut(s)	1300
HindIII	AAGCTT	3 cut(s)	78, 791, 801
HinfI	GANTC	5 cut(s)	191, 695, 1185, 1559, 1747
HpaII	CCGG	4 cut(s)	123, 775, 1152, 1475
HphI	GGTGA	5 cut(s)	167, 884, 1031, 1121, 1489
Hpy166II	GTNNAC	7 cut(s)	373, 394, 484, 1144, 1210, 1300, 1704
Hpy188I	TCNGA	8 cut(s)	241, 594, 673, 751, 765, 1063, 1419, 1465
Hpy8I	GTNNAC	7 cut(s)	373, 394, 484, 1144, 1210, 1300, 1704
Hpy99I	CGWCG	3 cut(s)	482, 769, 1301
HpyAV	CCTTC	7 cut(s)	96, 257, 667, 877, 1167, 1659, 1770
HpyCH4III	ACNGT	8 cut(s)	295, 755, 1096, 1266, 1297, 1445, 1512, 1726
HpyCH4IV	ACGT	6 cut(s)	480, 767, 951, 1005, 1496, 1700
HpyCH4V	TGCA	6 cut(s)	500, 722, 1341, 1543, 1604, 1691
HpyF10VI	GCNNNNNNNGC	5 cut(s)	311, 719, 836, 1097, 1407
HpyF3I	CTNAG	8 cut(s)	306, 494, 593, 693, 840, 1060, 1318, 1660
HpySE526I	ACGT	6 cut(s)	480, 767, 951, 1005, 1496, 1700
Hsp92II	CATG	7 cut(s)	368, 448, 640, 1543, 1555, 1636, 1691
HspAI	GCGC	1 cut(s)	162
Kpn2I	TCCGGA	1 cut(s)	122
KroI	GCCGGC	1 cut(s)	1151
KroNI	GCCGGC	1 cut(s)	1153
Ksp22I	TGATCA	1 cut(s)	241
Kzo9I	GATC	6 cut(s)	116, 241, 361, 938, 1481, 1533
LmnI	GCTCC	1 cut(s)	673
Lsp1109I	GCAGC	5 cut(s)	512, 879, 1087, 1419, 1743
LweI	GCATC	7 cut(s)	409, 755, 817, 1138, 1469, 1661, 1700
MaeI	CTAG	7 cut(s)	114, 260, 527, 617, 663, 797, 924
MaeII	ACGT	6 cut(s)	480, 767, 951, 1005, 1496, 1700
MaeIII	GTNAC	2 cut(s)	577, 1387
MalI	GATC	6 cut(s)	118, 243, 363, 940, 1483, 1535
MboI	GATC	6 cut(s)	116, 241, 361, 938, 1481, 1533
MboII	GAAGA	4 cut(s)	46, 747, 1300, 1462
MflI	RGATCY	2 cut(s)	938, 1481
MhlI	GDGCHC	1 cut(s)	322
MluCI	AATT	6 cut(s)	24, 45, 107, 897, 1636, 1791
MlyI	GAGTC	3 cut(s)	704, 1194, 1553
MmeI	TCCRAC	1 cut(s)	788
Mph1103I	ATGCAT	1 cut(s)	1693
MroI	TCCGGA	1 cut(s)	122
MroNI	GCCGGC	1 cut(s)	1151
MseI	TTAA	2 cut(s)	1608, 1671
MslI	CAYNNNNRTG	2 cut(s)	26, 1769
MspA1I	CMGCKG	1 cut(s)	1371
MspI	CCGG	4 cut(s)	123, 775, 1152, 1475
MspR9I	CCNGG	5 cut(s)	381, 490, 776, 1235, 1475
Mva1269I	GAATGC	1 cut(s)	1718
MvaI	CCWGG	3 cut(s)	381, 490, 1235
MvnI	CGCG	1 cut(s)	387
MwoI	GCNNNNNNNGC	5 cut(s)	311, 719, 836, 1097, 1407
NaeI	GCCGGC	1 cut(s)	1153
NciI	CCSGG	2 cut(s)	776, 1475
NcoI	CCATGG	1 cut(s)	636
NdeII	GATC	6 cut(s)	116, 241, 361, 938, 1481, 1533
NgoMIV	GCCGGC	1 cut(s)	1151
NlaIII	CATG	7 cut(s)	368, 448, 640, 1543, 1555, 1636, 1691
NlaIV	GGNNCC	5 cut(s)	268, 773, 904, 1192, 1294
NmuCI	GTSAC	2 cut(s)	577, 1387
NsbI	TGCGCA	1 cut(s)	163
NsiI	ATGCAT	1 cut(s)	1693
OliI	CACNNNNGTG	1 cut(s)	1769
PaeR7I	CTCGAG	3 cut(s)	315, 536, 684
PagI	TCATGA	2 cut(s)	364, 444
PcsI	WCGNNNNNNNCGW	1 cut(s)	477
PctI	GAATGC	1 cut(s)	1718
PdiI	GCCGGC	1 cut(s)	1153
PfeI	GAWTC	2 cut(s)	191, 1747
PflFI	GACNNNGTC	1 cut(s)	440
PkrI	GCNGC	8 cut(s)	502, 781, 894, 1102, 1409, 1456, 1459, 1758
PleI	GAGTC	3 cut(s)	703, 1193, 1553
PpsI	GAGTC	3 cut(s)	703, 1193, 1553
Ppu21I	YACGTR	1 cut(s)	952
PshAI	GACNNNNGTC	1 cut(s)	770
PsiI	TTATAA	1 cut(s)	1083
Psp124BI	GAGCTC	1 cut(s)	322
Psp6I	CCWGG	3 cut(s)	379, 488, 1233
PspFI	CCCAGC	1 cut(s)	959
PspGI	CCWGG	3 cut(s)	379, 488, 1233
PspN4I	GGNNCC	5 cut(s)	268, 773, 904, 1192, 1294
PspPI	GGNCC	4 cut(s)	283, 772, 777, 1154
PspXI	VCTCGAGB	1 cut(s)	315
PstNI	CAGNNNCTG	1 cut(s)	830
PsuI	RGATCY	2 cut(s)	938, 1481
PsyI	GACNNNGTC	1 cut(s)	440
RsaI	GTAC	5 cut(s)	922, 928, 977, 1619, 1631
RsaNI	GTAC	5 cut(s)	921, 927, 976, 1618, 1630
RseI	CAYNNNNRTG	2 cut(s)	26, 1769
SacI	GAGCTC	1 cut(s)	322
SalI	GTCGAC	1 cut(s)	1298
SaqAI	TTAA	2 cut(s)	1608, 1671
SatI	GCNGC	8 cut(s)	501, 780, 893, 1101, 1408, 1455, 1458, 1757
Sau3AI	GATC	6 cut(s)	116, 241, 361, 938, 1481, 1533
Sau96I	GGNCC	4 cut(s)	283, 772, 777, 1154
ScaI	AGTACT	1 cut(s)	928
SchI	GAGTC	3 cut(s)	704, 1194, 1553
ScrFI	CCNGG	5 cut(s)	381, 490, 776, 1235, 1475
SduI	GDGCHC	1 cut(s)	322
SfaNI	GCATC	7 cut(s)	409, 755, 817, 1138, 1469, 1661, 1700
SfcI	CTRYAG	1 cut(s)	423
Sfr274I	CTCGAG	3 cut(s)	315, 536, 684
SinI	GGWCC	2 cut(s)	283, 772
SlaI	CTCGAG	3 cut(s)	315, 536, 684
SmiMI	CAYNNNNRTG	2 cut(s)	26, 1769
SmlI	CTYRAG	7 cut(s)	58, 315, 404, 536, 684, 1395, 1707
SmoI	CTYRAG	7 cut(s)	58, 315, 404, 536, 684, 1395, 1707
SnaBI	TACGTA	1 cut(s)	952
SpeI	ACTAGT	1 cut(s)	923
Sse9I	AATT	6 cut(s)	24, 45, 107, 897, 1636, 1791
SsiI	CCGC	8 cut(s)	38, 349, 387, 780, 1361, 1371, 1454, 1457
SspMI	CTAG	7 cut(s)	114, 260, 527, 617, 663, 797, 924
SstI	GAGCTC	1 cut(s)	322
StyD4I	CCNGG	5 cut(s)	379, 488, 774, 1233, 1473
StyI	CCWWGG	3 cut(s)	636, 906, 1586
TaaI	ACNGT	8 cut(s)	295, 755, 1096, 1266, 1297, 1445, 1512, 1726
TaiI	ACGT	6 cut(s)	483, 770, 954, 1008, 1499, 1703
TaqII	GACCGA	1 cut(s)	271
TasI	AATT	6 cut(s)	24, 45, 107, 897, 1636, 1791
TatI	WGTACW	4 cut(s)	926, 975, 1617, 1629
TauI	GCSGC	3 cut(s)	782, 1457, 1460
TfiI	GAWTC	2 cut(s)	191, 1747
Tru1I	TTAA	2 cut(s)	1608, 1671
Tru9I	TTAA	2 cut(s)	1608, 1671
TscAI	CASTG	4 cut(s)	655, 697, 885, 1771
TseFI	GTSAC	2 cut(s)	577, 1387
TseI	GCWGC	5 cut(s)	500, 892, 1100, 1407, 1756
Tsp45I	GTSAC	2 cut(s)	577, 1387
TspDTI	ATGAA	5 cut(s)	1108, 1294, 1540, 1649, 1726
TspRI	CASTG	4 cut(s)	655, 697, 885, 1771
Tth111I	GACNNNGTC	1 cut(s)	440
VpaK11BI	GGWCC	2 cut(s)	283, 772
XagI	CCTNNNNNAGG	1 cut(s)	1663
XapI	RAATTY	3 cut(s)	45, 107, 1636
XbaI	TCTAGA	3 cut(s)	113, 526, 796
XhoI	CTCGAG	3 cut(s)	315, 536, 684
XmiI	GTMKAC	1 cut(s)	1299
XspI	CTAG	7 cut(s)	114, 260, 527, 617, 663, 797, 924
ZrmI	AGTACT	1 cut(s)	928
Zsp2I	ATGCAT	1 cut(s)	1693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.