Rmu_sc0040314.1_g000001

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0040314.1
OG0011630
Physical Location & Seq
Reverse (-)
1008 .. 1724
717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0040314.1_g000001.1.cds

Sequence Viewer

Length: 717 bp
atgactcgttttgcagctgctatgggtgatgggttcatcgctcgtgatataacatttgtgaacgatgccggcccttccaagcaccaagcagtcgcacttcgagtcggtgccgacaaatcagtcgtccaccgatgttccatcataggctaccaggacaccctctacactcactccaagcgacagttctaccgagaaaccgacatctatggaaccgtcgacttcatcttcggcaactcagccgtggttttccaaacttgcaacatccacccccgaaaaccgccctccagcggactaaagaactttgtcacagctcaaggccgaagcagctctaatcagaacacaggtatttcaatccacaactgtaagatagcggcggcatcagacctcttcccggtgagatccagctatgagacgtatcttgggagaccgtggaagcagtattcgagaacgatcatcatgcagtctttcatggatgactcgataaatcgagcagggtggtctccttggtctggtgggtctgcacttaacacattgtactatggagagtacatgaattctgggtctggggcatcaacctcaggaagggttaaatggggtggatatcatgcatcacttacgtccactcaagcagaggcattcacagttgggggtttcatatctgggaatctttggctgccttccactggggtgtcttttagttctggactaattggataa

Protein Analysis

238

Amino Acids

25.84

Weight (kDa)

9.4

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 216
AciI CCGC 4 cut(s) 278, 288, 371, 374
AclWI GGATC 1 cut(s) 393
AcsI RAATTY 1 cut(s) 553
AfaI GTAC 2 cut(s) 536, 548
AfiI CCNNNNNNNGG 5 cut(s) 287, 391, 509, 582, 683
AgsI TTSAA 1 cut(s) 351
AjnI CCWGG 1 cut(s) 150
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AleI CACNNNNGTG 1 cut(s) 686
AluBI AGCT 4 cut(s) 17, 311, 327, 405
AluI AGCT 4 cut(s) 17, 311, 327, 405
Alw26I GTCTC 3 cut(s) 404, 418, 504
AlwI GGATC 1 cut(s) 393
AoxI GGCC 2 cut(s) 70, 316
ApeKI GCWGC 4 cut(s) 14, 17, 324, 673
ApoI RAATTY 1 cut(s) 553
ArsI GACNNNNNNTTYG 4 cut(s) 108, 140, 209, 241
AspS9I GGNCC 1 cut(s) 71
AsuC2I CCSGG 1 cut(s) 392
AsuHPI GGTGA 2 cut(s) 38, 406
AxyI CCTNAGG 1 cut(s) 577
BanI GGYRCC 1 cut(s) 107
BauI CACGAG 1 cut(s) 42
BbvI GCAGC 4 cut(s) 4, 26, 336, 660
BccI CCATC 2 cut(s) 23, 146
BceAI ACGGC 1 cut(s) 224
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 1 cut(s) 152
BcnI CCSGG 1 cut(s) 392
BcoDI GTCTC 3 cut(s) 404, 418, 504
BisI GCNGC 6 cut(s) 15, 18, 325, 372, 375, 674
BlsI GCNGC 6 cut(s) 16, 19, 326, 373, 376, 675
Bme1390I CCNGG 2 cut(s) 152, 392
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 109, 211
BmrFI CCNGG 2 cut(s) 152, 392
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 4 cut(s) 55, 386, 578, 617
BmuI ACTGGG 1 cut(s) 693
BpmI CTGGAG 1 cut(s) 268
BpuEI CTTGAG 2 cut(s) 297, 609
BpuMI CCSGG 1 cut(s) 392
BsaI GGTCTC 2 cut(s) 418, 504
BsaJI CCNNGG 3 cut(s) 240, 428, 503
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 5 cut(s) 287, 391, 509, 582, 683
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 688
Bse21I CCTNAGG 1 cut(s) 577
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 3 cut(s) 240, 428, 503
BseGI GGATG 2 cut(s) 261, 478
BseLI CCNNNNNNNGG 5 cut(s) 287, 391, 509, 582, 683
BseMII CTCAG 2 cut(s) 249, 591
BseNI ACTGG 1 cut(s) 688
BseXI GCAGC 4 cut(s) 4, 26, 336, 660
BsgI GTGCAG 1 cut(s) 504
BshFI GGCC 2 cut(s) 72, 318
BshNI GGYRCC 1 cut(s) 107
BsiSI CCGG 2 cut(s) 69, 392
BslI CCNNNNNNNGG 5 cut(s) 287, 391, 509, 582, 683
BsmAI GTCTC 3 cut(s) 404, 418, 504
BsmBI CGTCTC 1 cut(s) 404
BsmI GAATGC 1 cut(s) 635
BsnI GGCC 2 cut(s) 72, 318
Bso31I GGTCTC 2 cut(s) 418, 504
Bsp143I GATC 2 cut(s) 398, 450
BspACI CCGC 4 cut(s) 278, 288, 371, 374
BspANI GGCC 2 cut(s) 72, 318
BspCNI CTCAG 2 cut(s) 248, 590
BspLI GGNNCC 2 cut(s) 109, 211
BspPI GGATC 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 107
BspTNI GGTCTC 2 cut(s) 418, 504
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 1 cut(s) 688
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 3 cut(s) 240, 428, 503
BssMI GATC 2 cut(s) 398, 450
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 503
Bst2BI CACGAG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 5 cut(s) 183, 214, 362, 429, 643
Bst6I CTCTTC 1 cut(s) 392
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 2 cut(s) 235, 577
BstDSI CCRYGG 2 cut(s) 240, 428
BstENI CCTNNNNNAGG 1 cut(s) 580
BstF5I GGATG 2 cut(s) 261, 478
BstKTI GATC 2 cut(s) 401, 453
BstMAI GTCTC 3 cut(s) 404, 418, 504
BstMBI GATC 2 cut(s) 398, 450
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstNI CCWGG 1 cut(s) 152
BstSCI CCNGG 2 cut(s) 150, 390
BstV1I GCAGC 4 cut(s) 4, 26, 336, 660
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
Bsu36I CCTNAGG 1 cut(s) 577
BsuRI GGCC 2 cut(s) 72, 318
BtgI CCRYGG 2 cut(s) 240, 428
BtgZI GCGATG 1 cut(s) 22
BtsCI GGATG 2 cut(s) 261, 478
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 1 cut(s) 70
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 2 cut(s) 535, 547
CviAII CATG 4 cut(s) 457, 469, 550, 605
CviJI RGCY 9 cut(s) 17, 72, 147, 239, 311, 318, 327, 405, 673
CviKI_1 RGCY 9 cut(s) 17, 72, 147, 239, 311, 318, 327, 405, 673
CviQI GTAC 2 cut(s) 535, 547
DdeI CTNAG 2 cut(s) 235, 577
DpnI GATC 2 cut(s) 400, 452
DpnII GATC 2 cut(s) 398, 450
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 503
Eco31I GGTCTC 2 cut(s) 418, 504
Eco32I GATATC 1 cut(s) 602
Eco81I CCTNAGG 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 580
EcoRI GAATTC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 150
EcoRV GATATC 1 cut(s) 602
EcoT14I CCWWGG 1 cut(s) 503
EcoT22I ATGCAT 1 cut(s) 610
ErhI CCWWGG 1 cut(s) 503
Esp3I CGTCTC 1 cut(s) 404
FaeI CATG 4 cut(s) 460, 472, 553, 608
FatI CATG 4 cut(s) 456, 468, 549, 604
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 6 cut(s) 15, 18, 325, 372, 375, 674
FokI GGATG 2 cut(s) 248, 485
Fsp4HI GCNGC 6 cut(s) 15, 18, 325, 372, 375, 674
GluI GCNGC 6 cut(s) 15, 18, 325, 372, 375, 674
GsuI CTGGAG 1 cut(s) 268
HaeIII GGCC 2 cut(s) 72, 318
HapII CCGG 2 cut(s) 69, 392
Hin1II CATG 4 cut(s) 460, 472, 553, 608
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 4 cut(s) 4, 102, 476, 664
HpaII CCGG 2 cut(s) 69, 392
HphI GGTGA 2 cut(s) 38, 406
Hpy166II GTNNAC 4 cut(s) 61, 127, 217, 621
Hpy188I TCNGA 2 cut(s) 336, 382
Hpy188III TCNNGA 4 cut(s) 44, 444, 579, 702
Hpy8I GTNNAC 4 cut(s) 61, 127, 217, 621
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 3 cut(s) 84, 576, 687
HpyCH4III ACNGT 5 cut(s) 183, 214, 362, 429, 643
HpyCH4IV ACGT 2 cut(s) 413, 617
HpyCH4V TGCA 5 cut(s) 14, 258, 460, 521, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpyF3I CTNAG 2 cut(s) 235, 577
HpySE526I ACGT 2 cut(s) 413, 617
Hsp92II CATG 4 cut(s) 460, 472, 553, 608
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Kzo9I GATC 2 cut(s) 398, 450
Lsp1109I GCAGC 4 cut(s) 4, 26, 336, 660
LweI GCATC 4 cut(s) 55, 386, 578, 617
MaeII ACGT 2 cut(s) 413, 617
MaeIII GTNAC 1 cut(s) 304
MalI GATC 2 cut(s) 400, 452
MboI GATC 2 cut(s) 398, 450
MboII GAAGA 2 cut(s) 217, 379
MflI RGATCY 1 cut(s) 398
MluCI AATT 2 cut(s) 553, 708
MlyI GAGTC 2 cut(s) 111, 470
MnlI CCTC 5 cut(s) 170, 292, 395, 586, 625
Mph1103I ATGCAT 1 cut(s) 610
MroNI GCCGGC 1 cut(s) 68
MseI TTAA 2 cut(s) 525, 588
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 2 cut(s) 17, 288
MspI CCGG 2 cut(s) 69, 392
MspR9I CCNGG 2 cut(s) 152, 392
Mva1269I GAATGC 1 cut(s) 635
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 324
NaeI GCCGGC 1 cut(s) 70
NciI CCSGG 1 cut(s) 392
NdeII GATC 2 cut(s) 398, 450
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 4 cut(s) 460, 472, 553, 608
NlaIV GGNNCC 2 cut(s) 109, 211
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 610
OliI CACNNNNGTG 1 cut(s) 686
PctI GAATGC 1 cut(s) 635
PdiI GCCGGC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 664
PkrI GCNGC 6 cut(s) 16, 19, 326, 373, 376, 675
PleI GAGTC 2 cut(s) 110, 470
PpsI GAGTC 2 cut(s) 110, 470
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 2 cut(s) 109, 211
PspPI GGNCC 1 cut(s) 71
PsuI RGATCY 1 cut(s) 398
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 536, 548
RsaNI GTAC 2 cut(s) 535, 547
RseI CAYNNNNRTG 1 cut(s) 686
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 2 cut(s) 525, 588
SatI GCNGC 6 cut(s) 15, 18, 325, 372, 375, 674
Sau3AI GATC 2 cut(s) 398, 450
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 2 cut(s) 111, 470
ScrFI CCNGG 2 cut(s) 152, 392
SetI ASST 9 cut(s) 19, 313, 329, 346, 387, 407, 416, 578, 620
SfaNI GCATC 4 cut(s) 55, 386, 578, 617
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 2 cut(s) 312, 624
SmoI CTYRAG 2 cut(s) 312, 624
Sse9I AATT 2 cut(s) 553, 708
SsiI CCGC 4 cut(s) 278, 288, 371, 374
StyD4I CCNGG 2 cut(s) 150, 390
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 5 cut(s) 183, 214, 362, 429, 643
TaiI ACGT 2 cut(s) 416, 620
TaqI TCGA 5 cut(s) 100, 216, 443, 479, 487
TasI AATT 2 cut(s) 553, 708
TatI WGTACW 2 cut(s) 534, 546
TauI GCSGC 2 cut(s) 374, 377
TfiI GAWTC 1 cut(s) 664
Tru1I TTAA 2 cut(s) 525, 588
Tru9I TTAA 2 cut(s) 525, 588
TscAI CASTG 1 cut(s) 688
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 4 cut(s) 14, 17, 324, 673
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 5 cut(s) 25, 211, 457, 566, 643
TspRI CASTG 1 cut(s) 688
XagI CCTNNNNNAGG 1 cut(s) 580
XapI RAATTY 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 216
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.