FvH4_4g12140

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011630
Physical Location & Seq
Reverse (-)
15822910 .. 15826316
3407 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12140.t1

Sequence Viewer

Length: 1740 bp
ATGTCTGTCTGTCTCTCTACCATAAGCATGTTCAAAATTAAGTTGGCTGTAGCAATTTTATTCTTCTCAAGTATTTTTATAGTAGAGGCTTCAACTTCACAATCCCATTTCTCCAAATTCACTAGATCATCCGGAGAACTTTCCCATGAAGAAGCCATTTCTTCTCTCTGCGAACATACCCCTCACCCAGCAACTTGTGAATCCACTCTCCTCTCCTCCTCATCCTCCTCCTCCTCATCATCATCATCATCTACAAGAGGCACCTTAGAGGTCGCCTTTCTCCAGTCGGTCCATTCCACTGTTGCGAAAGCTCAGCACGCTCGAGCTCTCGCCTATAATCTCACCATTTCCCACCGCCAGAATAATGACGATAGTTCTGTTCACTTCCGCGATGGTAGTGTGAGCGACTGCCTTGAGCTTCTCGACGATGCTGTAGATATGCTCGACAATGTCATGACGACATCGTTGTCGAAACCTCACAACGATGACGACGTCCACACCTGGCTCAGTGCAGCTCTTACGAACCAAGAAACTTGTCTGGAGAGCCTCGAGAAAGACAAGTTCAAGACAGACAAAAGCATTATGGACGCCACGGCTCAAAATCTGAGCCAATACATAAGCAATTCCCTAGCACTTTTCATGCTTACCATGGGGTCAAAATCAAAGGAAGCAAAAAATGCAAACCCTAATAGAAAGCTCTTGAGTTTCCCGACGTGGGTTCCGGCAGCGGAGAGGAAACTTCTAGAAGCATCTGTGGAAGAGATAGAAGCAGATGCTGTGGTGGCTCAGGATGGGAGTGGGACGCACAAGAGCATTGGTGAGGCACTCAAGGGTTTGCTTCTAACTGGATCCTTGGAGAGCAGTGGTACTGGGGGCAGATCTGTAATTCACGTAACGGCTGGGACTTACCACGAATACATAAATATACCCACAAAGCAAAAGAACGTCATGTTGATTGGTGATGGAGTAGGGAAAACCATCATTGTGGGCAATCGGAACTCTGATGATGGCTGGACAACTTATAAGACTGCCACTGTGGCTGCAATGGGAGATGGGTTTATCGCACGTGATATAACAATTGTGAACAATGCCGGGCCTTCCAAGCACCAAGCAGTTGCACTTCGAGTCGGTGCCGACAAATCCGTCGTCCACCGATGTTCCATCATGGGCTACCAGGACACCCTCTACACTCACTCCAAGCGACAGTTCTACCGAGAAACTGACATCTATGGAACTGTCGACTTCATCTTTGGCAACTCCGCTGTCGTCTTCCAAACTTGCAACATCTACCCTCGGAAACCGGCCTCCGGCGGGCTCAAGAACTTTGTCACAGCTCAAGGTCGCAGCAGCCCTGACCAGAACACAGGCATTTCAATCCACAACTGTAAGATAGCGGCGGCGTCGGACCTCGCCCCGGTGAAATCGAGCTACCAGACGTATCTTGGGAGACCGTGGAAGCAGTATTCGAGGACGATCGTCATGCAGTCTTTCTTGGATGACTCGATAAATCGAGCTGGGTGGTCTCCTTGGTCTGGTGGGTTTGCACTCAACACATTGTACTATGGAGAGTACATGAATTCTGGGTCTGGGGCATCAACCTCAGGAAGGGTTAAATGGGGTGGATATCATGCATCACTTACGTCCACTCAAGCACAAGCATTCACAGTTGGGGGTTTCATATCTGGGAATCTTTGGTTGCCTTCCACTGGGGTGTCTTTTAGCTCTGGACTCATTGGATAA

Protein Analysis

580

Amino Acids

62.35

Weight (kDa)

6.81

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 52 - 212 7.4e-27 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 258 - 563 1.2e-135 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
AasI GACNNNNNNGTC 2 cut(s) 466, 1142
AatII GACGTC 1 cut(s) 495
AccB1I GGYRCC 2 cut(s) 260, 1130
AccI GTMKAC 1 cut(s) 1239
AccII CGCG 1 cut(s) 390
AccIII TCCGGA 1 cut(s) 131
AciI CCGC 7 cut(s) 355, 388, 728, 1260, 1311, 1394, 1397
AclWI GGATC 2 cut(s) 843, 856
AcsI RAATTY 2 cut(s) 116, 1576
AcvI CACGTG 1 cut(s) 1067
AcyI GRCGYC 3 cut(s) 492, 588, 1400
AfaI GTAC 3 cut(s) 868, 1559, 1571
AfiI CCNNNNNNNGG 7 cut(s) 715, 1307, 1311, 1414, 1532, 1605, 1706
AgsI TTSAA 4 cut(s) 34, 93, 565, 1374
AjiI CACGTC 1 cut(s) 714
AjnI CCWGG 2 cut(s) 500, 1173
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AleI CACNNNNGTG 1 cut(s) 1709
AluBI AGCT 9 cut(s) 311, 326, 418, 515, 697, 1334, 1428, 1514, 1722
AluI AGCT 9 cut(s) 311, 326, 418, 515, 697, 1334, 1428, 1514, 1722
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 3 cut(s) 17, 1441, 1527
AlwI GGATC 2 cut(s) 843, 856
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 2 cut(s) 321, 548
Aor13HI TCCGGA 1 cut(s) 131
AoxI GGCC 2 cut(s) 1094, 1302
ApeKI GCWGC 5 cut(s) 512, 725, 1040, 1344, 1347
ApoI RAATTY 2 cut(s) 116, 1576
ArsI GACNNNNNNTTYG 4 cut(s) 1131, 1163, 1232, 1264
AspS9I GGNCC 3 cut(s) 289, 1094, 1405
AsuC2I CCSGG 2 cut(s) 1093, 1415
AsuHPI GGTGA 5 cut(s) 176, 334, 830, 971, 1429
AvaI CYCGRG 2 cut(s) 321, 548
AvaII GGWCC 2 cut(s) 289, 1405
AxyI CCTNAGG 1 cut(s) 1600
BamHI GGATCC 1 cut(s) 848
BanI GGYRCC 2 cut(s) 260, 1130
BanII GRGCYC 2 cut(s) 328, 1317
BbrPI CACGTG 1 cut(s) 1067
BbsI GAAGAC 1 cut(s) 1261
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 5 cut(s) 524, 737, 1027, 1356, 1359
BccI CCATC 7 cut(s) 386, 785, 956, 986, 1001, 1046, 1169
BceAI ACGGC 2 cut(s) 609, 912
BcgI CGANNNNNNTGC 2 cut(s) 1462, 1496
BciT130I CCWGG 2 cut(s) 502, 1175
BcnI CCSGG 2 cut(s) 1093, 1415
BcoDI GTCTC 3 cut(s) 17, 1441, 1527
BfaI CTAG 3 cut(s) 123, 629, 743
BfmI CTRYAG 2 cut(s) 48, 432
BglI GCCNNNNNGGC 1 cut(s) 1037
BglII AGATCT 1 cut(s) 878
BisI GCNGC 7 cut(s) 513, 726, 1041, 1345, 1348, 1395, 1398
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 7 cut(s) 514, 727, 1042, 1346, 1349, 1396, 1399
Bme1390I CCNGG 4 cut(s) 502, 1093, 1175, 1415
Bme18I GGWCC 2 cut(s) 289, 1405
BmeT110I CYCGRG 2 cut(s) 321, 548
BmgBI CACGTC 1 cut(s) 714
BmgT120I GGNCC 3 cut(s) 289, 1094, 1405
BmiI GGNNCC 4 cut(s) 262, 720, 850, 1132
BmrFI CCNGG 4 cut(s) 502, 1093, 1175, 1415
BmrI ACTGGG 2 cut(s) 879, 1716
BmsI GCATC 5 cut(s) 418, 758, 763, 1601, 1640
BmuI ACTGGG 2 cut(s) 879, 1716
BoxI GACNNNNGTC 1 cut(s) 1475
BpiI GAAGAC 1 cut(s) 1261
BpmI CTGGAG 2 cut(s) 266, 560
Bpu10I CCTNAGC 1 cut(s) 786
Bpu1102I GCTNAGC 1 cut(s) 312
BpuEI CTTGAG 7 cut(s) 52, 434, 721, 812, 1301, 1320, 1632
BpuMI CCSGG 2 cut(s) 1093, 1415
BsaAI YACGTR 2 cut(s) 892, 1067
BsaHI GRCGYC 3 cut(s) 492, 588, 1400
BsaI GGTCTC 2 cut(s) 1441, 1527
BsaJI CCNNGG 7 cut(s) 591, 648, 852, 1292, 1413, 1451, 1526
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 2 cut(s) 1178, 1208
Bsc4I CCNNNNNNNGG 7 cut(s) 715, 1307, 1311, 1414, 1532, 1605, 1706
Bse118I RCCGGY 1 cut(s) 1300
Bse1I ACTGG 4 cut(s) 283, 850, 874, 1711
Bse21I CCTNAGG 1 cut(s) 1600
Bse3DI GCAATG 1 cut(s) 1050
BseAI TCCGGA 1 cut(s) 131
BseBI CCWGG 2 cut(s) 502, 1175
BseDI CCNNGG 7 cut(s) 591, 648, 852, 1292, 1413, 1451, 1526
BseGI GGATG 4 cut(s) 128, 221, 796, 1501
BseLI CCNNNNNNNGG 7 cut(s) 715, 1307, 1311, 1414, 1532, 1605, 1706
BseMI GCAATG 1 cut(s) 1050
BseMII CTCAG 5 cut(s) 326, 520, 596, 800, 1614
BseNI ACTGG 4 cut(s) 283, 850, 874, 1711
BseRI GAGGAG 6 cut(s) 200, 205, 208, 217, 220, 223
BseXI GCAGC 5 cut(s) 524, 737, 1027, 1356, 1359
BseYI CCCAGC 3 cut(s) 187, 899, 1514
BsgI GTGCAG 1 cut(s) 531
Bsh1236I CGCG 1 cut(s) 390
Bsh1285I CGRYCG 1 cut(s) 1476
BshFI GGCC 2 cut(s) 1096, 1304
BshNI GGYRCC 2 cut(s) 260, 1130
BsiEI CGRYCG 1 cut(s) 1476
BsiHKAI GWGCWC 1 cut(s) 328
BsiHKCI CYCGRG 2 cut(s) 321, 548
BsiSI CCGG 6 cut(s) 132, 722, 1092, 1301, 1308, 1415
BslFI GGGAC 2 cut(s) 814, 916
BslI CCNNNNNNNGG 7 cut(s) 715, 1307, 1311, 1414, 1532, 1605, 1706
BsmAI GTCTC 3 cut(s) 17, 1441, 1527
BsmFI GGGAC 2 cut(s) 814, 916
BsmI GAATGC 1 cut(s) 1658
BsnI GGCC 2 cut(s) 1096, 1304
Bso31I GGTCTC 2 cut(s) 1441, 1527
BsoBI CYCGRG 2 cut(s) 321, 548
Bsp1286I GDGCHC 2 cut(s) 328, 1317
Bsp13I TCCGGA 1 cut(s) 131
Bsp143I GATC 4 cut(s) 125, 848, 878, 1473
Bsp1720I GCTNAGC 1 cut(s) 312
Bsp19I CCATGG 1 cut(s) 648
BspACI CCGC 7 cut(s) 355, 388, 728, 1260, 1311, 1394, 1397
BspANI GGCC 2 cut(s) 1096, 1304
BspCNI CTCAG 5 cut(s) 325, 519, 597, 799, 1613
BspEI TCCGGA 1 cut(s) 131
BspFNI CGCG 1 cut(s) 390
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 4 cut(s) 262, 720, 850, 1132
BspPI GGATC 2 cut(s) 843, 856
BspT107I GGYRCC 2 cut(s) 260, 1130
BspTNI GGTCTC 2 cut(s) 1441, 1527
BsrDI GCAATG 1 cut(s) 1050
BsrFI RCCGGY 1 cut(s) 1300
BsrI ACTGG 4 cut(s) 283, 850, 874, 1711
BssAI RCCGGY 1 cut(s) 1300
BssECI CCNNGG 7 cut(s) 591, 648, 852, 1292, 1413, 1451, 1526
BssMI GATC 4 cut(s) 125, 848, 878, 1473
BssNI GRCGYC 3 cut(s) 492, 588, 1400
BssT1I CCWWGG 3 cut(s) 648, 852, 1526
Bst2UI CCWGG 2 cut(s) 502, 1175
Bst4CI ACNGT 7 cut(s) 301, 1036, 1206, 1237, 1385, 1452, 1666
Bst6I CTCTTC 1 cut(s) 753
BstACI GRCGYC 3 cut(s) 492, 588, 1400
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBAI YACGTR 2 cut(s) 892, 1067
BstC8I GCNNGC 2 cut(s) 318, 1313
BstDEI CTNAG 6 cut(s) 265, 312, 506, 605, 786, 1600
BstDSI CCRYGG 3 cut(s) 591, 648, 1451
BstENI CCTNNNNNAGG 1 cut(s) 1603
BstF5I GGATG 4 cut(s) 128, 221, 796, 1501
BstFNI CGCG 1 cut(s) 390
BstKTI GATC 4 cut(s) 128, 851, 881, 1476
BstMAI GTCTC 3 cut(s) 17, 1441, 1527
BstMBI GATC 4 cut(s) 125, 848, 878, 1473
BstMCI CGRYCG 1 cut(s) 1476
BstMWI GCNNNNNNNGC 5 cut(s) 317, 677, 782, 1037, 1102
BstNI CCWGG 2 cut(s) 502, 1175
BstNSI RCATGY 1 cut(s) 31
BstPAI GACNNNNGTC 1 cut(s) 1475
BstSCI CCNGG 4 cut(s) 500, 1091, 1173, 1413
BstSFI CTRYAG 2 cut(s) 48, 432
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 5 cut(s) 524, 737, 1027, 1356, 1359
BstV2I GAAGAC 1 cut(s) 1261
BstX2I RGATCY 2 cut(s) 848, 878
BstXI CCANNNNNNTGG 1 cut(s) 985
BstYI RGATCY 2 cut(s) 848, 878
Bsu36I CCTNAGG 1 cut(s) 1600
BsuRI GGCC 2 cut(s) 1096, 1304
BtgI CCRYGG 3 cut(s) 591, 648, 1451
BtgZI GCGATG 1 cut(s) 405
BtrI CACGTC 1 cut(s) 714
BtsCI GGATG 4 cut(s) 128, 221, 796, 1501
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 5 cut(s) 297, 514, 868, 1032, 1704
Cac8I GCNNGC 2 cut(s) 318, 1313
CaiI CAGNNNCTG 1 cut(s) 776
CciI TCATGA 1 cut(s) 453
Cfr10I RCCGGY 1 cut(s) 1300
Cfr13I GGNCC 3 cut(s) 289, 1094, 1405
CseI GACGC 3 cut(s) 596, 811, 1389
Csp6I GTAC 3 cut(s) 867, 1558, 1570
CviQI GTAC 3 cut(s) 867, 1558, 1570
DdeI CTNAG 6 cut(s) 265, 312, 506, 605, 786, 1600
DpnI GATC 4 cut(s) 127, 850, 880, 1475
DpnII GATC 4 cut(s) 125, 848, 878, 1473
DrdI GACNNNNNNGTC 2 cut(s) 466, 1142
DseDI GACNNNNNNGTC 2 cut(s) 466, 1142
Eam1104I CTCTTC 1 cut(s) 753
EarI CTCTTC 1 cut(s) 753
Ecl136II GAGCTC 1 cut(s) 326
Eco130I CCWWGG 3 cut(s) 648, 852, 1526
Eco24I GRGCYC 2 cut(s) 328, 1317
Eco31I GGTCTC 2 cut(s) 1441, 1527
Eco32I GATATC 1 cut(s) 1625
Eco47I GGWCC 2 cut(s) 289, 1405
Eco53kI GAGCTC 1 cut(s) 326
Eco72I CACGTG 1 cut(s) 1067
Eco81I CCTNAGG 1 cut(s) 1600
Eco88I CYCGRG 2 cut(s) 321, 548
EcoICRI GAGCTC 1 cut(s) 326
EcoNI CCTNNNNNAGG 1 cut(s) 1603
EcoRI GAATTC 1 cut(s) 1576
EcoRII CCWGG 2 cut(s) 500, 1173
EcoRV GATATC 1 cut(s) 1625
EcoT14I CCWWGG 3 cut(s) 648, 852, 1526
EcoT22I ATGCAT 1 cut(s) 1633
EcoT38I GRGCYC 2 cut(s) 328, 1317
ErhI CCWWGG 3 cut(s) 648, 852, 1526
FaqI GGGAC 2 cut(s) 814, 916
FauI CCCGC 1 cut(s) 1304
FblI GTMKAC 1 cut(s) 1239
Fnu4HI GCNGC 7 cut(s) 513, 726, 1041, 1345, 1348, 1395, 1398
FokI GGATG 4 cut(s) 115, 208, 803, 1508
FriOI GRGCYC 2 cut(s) 328, 1317
Fsp4HI GCNGC 7 cut(s) 513, 726, 1041, 1345, 1348, 1395, 1398
FspBI CTAG 3 cut(s) 123, 629, 743
GluI GCNGC 7 cut(s) 513, 726, 1041, 1345, 1348, 1395, 1398
GsaI CCCAGC 3 cut(s) 191, 903, 1518
GsuI CTGGAG 2 cut(s) 266, 560
HaeIII GGCC 2 cut(s) 1096, 1304
HapII CCGG 6 cut(s) 132, 722, 1092, 1301, 1308, 1415
HgaI GACGC 3 cut(s) 596, 811, 1389
Hin1I GRCGYC 3 cut(s) 492, 588, 1400
HincII GTYRAC 1 cut(s) 1240
HindII GTYRAC 1 cut(s) 1240
HinfI GANTC 5 cut(s) 200, 1125, 1499, 1687, 1728
HpaII CCGG 6 cut(s) 132, 722, 1092, 1301, 1308, 1415
HphI GGTGA 5 cut(s) 176, 334, 830, 971, 1429
Hpy166II GTNNAC 6 cut(s) 382, 496, 1084, 1150, 1240, 1644
Hpy188I TCNGA 5 cut(s) 606, 996, 1003, 1296, 1405
Hpy8I GTNNAC 6 cut(s) 382, 496, 1084, 1150, 1240, 1644
Hpy99I CGWCG 5 cut(s) 428, 494, 715, 1148, 1405
HpyAV CCTTC 3 cut(s) 1107, 1599, 1710
HpyCH4III ACNGT 7 cut(s) 301, 1036, 1206, 1237, 1385, 1452, 1666
HpyCH4IV ACGT 7 cut(s) 492, 713, 891, 945, 1066, 1436, 1640
HpyCH4V TGCA 8 cut(s) 512, 680, 1043, 1118, 1281, 1483, 1544, 1631
HpyF10VI GCNNNNNNNGC 5 cut(s) 317, 677, 782, 1037, 1102
HpyF3I CTNAG 6 cut(s) 265, 312, 506, 605, 786, 1600
HpySE526I ACGT 7 cut(s) 492, 713, 891, 945, 1066, 1436, 1640
Hsp92I GRCGYC 3 cut(s) 492, 588, 1400
Kpn2I TCCGGA 1 cut(s) 131
Kzo9I GATC 4 cut(s) 125, 848, 878, 1473
Lsp1109I GCAGC 5 cut(s) 524, 737, 1027, 1356, 1359
LweI GCATC 5 cut(s) 418, 758, 763, 1601, 1640
MaeI CTAG 3 cut(s) 123, 629, 743
MaeII ACGT 7 cut(s) 492, 713, 891, 945, 1066, 1436, 1640
MaeIII GTNAC 2 cut(s) 892, 1327
MalI GATC 4 cut(s) 127, 850, 880, 1475
MboI GATC 4 cut(s) 125, 848, 878, 1473
MboII GAAGA 5 cut(s) 55, 153, 161, 770, 1261
MfeI CAATTG 1 cut(s) 1077
MflI RGATCY 2 cut(s) 848, 878
MhlI GDGCHC 2 cut(s) 328, 1317
MluCI AATT 7 cut(s) 36, 54, 116, 622, 885, 1077, 1576
MlyI GAGTC 3 cut(s) 1134, 1493, 1722
MmeI TCCRAC 1 cut(s) 1383
Mph1103I ATGCAT 1 cut(s) 1633
MroI TCCGGA 1 cut(s) 131
MseI TTAA 2 cut(s) 39, 1611
MslI CAYNNNNRTG 4 cut(s) 26, 483, 983, 1709
MspA1I CMGCKG 2 cut(s) 728, 1262
MspI CCGG 6 cut(s) 132, 722, 1092, 1301, 1308, 1415
MspR9I CCNGG 4 cut(s) 502, 1093, 1175, 1415
MunI CAATTG 1 cut(s) 1077
Mva1269I GAATGC 1 cut(s) 1658
MvaI CCWGG 2 cut(s) 502, 1175
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 5 cut(s) 317, 677, 782, 1037, 1102
NciI CCSGG 2 cut(s) 1093, 1415
NcoI CCATGG 1 cut(s) 648
NdeII GATC 4 cut(s) 125, 848, 878, 1473
NlaIV GGNNCC 4 cut(s) 262, 720, 850, 1132
NmuCI GTSAC 1 cut(s) 1327
NsiI ATGCAT 1 cut(s) 1633
NspI RCATGY 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 1709
PaeR7I CTCGAG 2 cut(s) 321, 548
PagI TCATGA 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PctI GAATGC 1 cut(s) 1658
PfeI GAWTC 2 cut(s) 200, 1687
PflFI GACNNNGTC 2 cut(s) 449, 491
PkrI GCNGC 7 cut(s) 514, 727, 1042, 1346, 1349, 1396, 1399
Ple19I CGATCG 1 cut(s) 1476
PleI GAGTC 3 cut(s) 1133, 1493, 1722
PmaCI CACGTG 1 cut(s) 1067
PmlI CACGTG 1 cut(s) 1067
PpsI GAGTC 3 cut(s) 1133, 1493, 1722
Ppu21I YACGTR 2 cut(s) 892, 1067
PshAI GACNNNNGTC 1 cut(s) 1475
PsiI TTATAA 1 cut(s) 1023
Psp124BI GAGCTC 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 500, 1173
PspCI CACGTG 1 cut(s) 1067
PspFI CCCAGC 3 cut(s) 187, 899, 1514
PspGI CCWGG 2 cut(s) 500, 1173
PspN4I GGNNCC 4 cut(s) 262, 720, 850, 1132
PspPI GGNCC 3 cut(s) 289, 1094, 1405
PspXI VCTCGAGB 1 cut(s) 321
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 2 cut(s) 848, 878
PsyI GACNNNGTC 2 cut(s) 449, 491
PvuI CGATCG 1 cut(s) 1476
RsaI GTAC 3 cut(s) 868, 1559, 1571
RsaNI GTAC 3 cut(s) 867, 1558, 1570
RseI CAYNNNNRTG 4 cut(s) 26, 483, 983, 1709
SacI GAGCTC 1 cut(s) 328
SalI GTCGAC 1 cut(s) 1238
SaqAI TTAA 2 cut(s) 39, 1611
SatI GCNGC 7 cut(s) 513, 726, 1041, 1345, 1348, 1395, 1398
Sau3AI GATC 4 cut(s) 125, 848, 878, 1473
Sau96I GGNCC 3 cut(s) 289, 1094, 1405
SchI GAGTC 3 cut(s) 1134, 1493, 1722
ScrFI CCNGG 4 cut(s) 502, 1093, 1175, 1415
SduI GDGCHC 2 cut(s) 328, 1317
SfaNI GCATC 5 cut(s) 418, 758, 763, 1601, 1640
SfcI CTRYAG 2 cut(s) 48, 432
Sfr274I CTCGAG 2 cut(s) 321, 548
SinI GGWCC 2 cut(s) 289, 1405
SlaI CTCGAG 2 cut(s) 321, 548
SmiMI CAYNNNNRTG 4 cut(s) 26, 483, 983, 1709
SmlI CTYRAG 9 cut(s) 67, 321, 413, 548, 700, 827, 1316, 1335, 1647
SmoI CTYRAG 9 cut(s) 67, 321, 413, 548, 700, 827, 1316, 1335, 1647
Sse9I AATT 7 cut(s) 36, 54, 116, 622, 885, 1077, 1576
SsiI CCGC 7 cut(s) 355, 388, 728, 1260, 1311, 1394, 1397
SspMI CTAG 3 cut(s) 123, 629, 743
SstI GAGCTC 1 cut(s) 328
StyD4I CCNGG 4 cut(s) 500, 1091, 1173, 1413
StyI CCWWGG 3 cut(s) 648, 852, 1526
TaaI ACNGT 7 cut(s) 301, 1036, 1206, 1237, 1385, 1452, 1666
TaiI ACGT 7 cut(s) 495, 716, 894, 948, 1069, 1439, 1643
TaqII GACCGA 1 cut(s) 277
TasI AATT 7 cut(s) 36, 54, 116, 622, 885, 1077, 1576
TatI WGTACW 2 cut(s) 1557, 1569
TauI GCSGC 2 cut(s) 1397, 1400
TfiI GAWTC 2 cut(s) 200, 1687
Tru1I TTAA 2 cut(s) 39, 1611
Tru9I TTAA 2 cut(s) 39, 1611
TscAI CASTG 5 cut(s) 304, 514, 868, 1039, 1711
TseFI GTSAC 1 cut(s) 1327
TseI GCWGC 5 cut(s) 512, 725, 1040, 1344, 1347
Tsp45I GTSAC 1 cut(s) 1327
TspDTI ATGAA 5 cut(s) 162, 628, 1234, 1589, 1666
TspGWI ACGGA 1 cut(s) 1132
TspRI CASTG 5 cut(s) 304, 514, 868, 1039, 1711
Tth111I GACNNNGTC 2 cut(s) 449, 491
VpaK11BI GGWCC 2 cut(s) 289, 1405
XagI CCTNNNNNAGG 1 cut(s) 1603
XapI RAATTY 2 cut(s) 116, 1576
XbaI TCTAGA 1 cut(s) 742
XceI RCATGY 1 cut(s) 31
XcmI CCANNNNNNNNNTGG 1 cut(s) 1439
XhoI CTCGAG 2 cut(s) 321, 548
XmiI GTMKAC 1 cut(s) 1239
XspI CTAG 3 cut(s) 123, 629, 743
ZraI GACGTC 1 cut(s) 493
Zsp2I ATGCAT 1 cut(s) 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.